Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026282.01.T01 | XP_024639246.1 | 90.972 | 144 | 5 | 1 | 1 | 136 | 120 | 263 | 6.15E-87 | 264 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026282.01.T01 | Q9C544 | 65.734 | 143 | 41 | 1 | 2 | 136 | 174 | 316 | 5.94E-61 | 192 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026282.01.T01 | A0A072UEK4 | 90.972 | 144 | 5 | 1 | 1 | 136 | 175 | 318 | 5.86e-87 | 265 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180001588.01 | MsG0580026282.01 | 0.811741 | 6.015040e-51 | 3.753251e-48 |
| MsG0180004124.01 | MsG0580026282.01 | -0.814944 | 1.190324e-51 | 8.096391e-49 |
| MsG0180004125.01 | MsG0580026282.01 | -0.818126 | 2.306907e-52 | 1.711971e-49 |
| MsG0180004828.01 | MsG0580026282.01 | 0.801187 | 1.013579e-48 | 4.809951e-46 |
| MsG0280010617.01 | MsG0580026282.01 | 0.823446 | 1.379338e-53 | 1.187881e-50 |
| MsG0380012818.01 | MsG0580026282.01 | 0.822266 | 2.597510e-53 | 2.163475e-50 |
| MsG0480021151.01 | MsG0580026282.01 | -0.818567 | 1.832605e-52 | 1.376616e-49 |
| MsG0480021773.01 | MsG0580026282.01 | 0.801132 | 1.040096e-48 | 4.929181e-46 |
| MsG0480022378.01 | MsG0580026282.01 | 0.800362 | 1.493445e-48 | 6.940699e-46 |
| MsG0480023375.01 | MsG0580026282.01 | -0.822099 | 2.839009e-53 | 2.353221e-50 |
| MsG0480023532.01 | MsG0580026282.01 | 0.801297 | 9.624417e-49 | 4.580004e-46 |
| MsG0380015828.01 | MsG0580026282.01 | -0.839994 | 1.135252e-57 | 1.595200e-54 |
| MsG0380016065.01 | MsG0580026282.01 | -0.812713 | 3.691318e-51 | 2.364072e-48 |
| MsG0380016250.01 | MsG0580026282.01 | 0.805818 | 1.110958e-49 | 5.935380e-47 |
| MsG0380016819.01 | MsG0580026282.01 | 0.802608 | 5.175569e-49 | 2.546194e-46 |
| MsG0580026282.01 | MsG0580027854.01 | 0.815922 | 7.210493e-52 | 5.037496e-49 |
| MsG0580026282.01 | MsG0580030141.01 | 0.801357 | 9.354303e-49 | 4.458223e-46 |
| MsG0580026282.01 | MsG0580030179.01 | 0.802244 | 6.149837e-49 | 2.997721e-46 |
| MsG0580026282.01 | MsG0680035805.01 | 0.810667 | 1.028737e-50 | 6.239228e-48 |
| MsG0580026282.01 | MsG0780036553.01 | 0.816643 | 4.975116e-52 | 3.545055e-49 |
| MsG0580026282.01 | MsG0780040799.01 | 0.809368 | 1.958322e-50 | 1.147697e-47 |
| MsG0580026282.01 | MsG0780041118.01 | 0.801620 | 8.263558e-49 | 3.964379e-46 |
| MsG0580026282.01 | MsG0780041807.01 | -0.802269 | 6.078742e-49 | 2.964873e-46 |
| MsG0580026282.01 | MsG0880044317.01 | -0.834190 | 3.458327e-56 | 4.073296e-53 |
| MsG0580026282.01 | MsG0880044436.01 | -0.807567 | 4.746846e-50 | 2.652679e-47 |
| MsG0580026282.01 | MsG0880045647.01 | 0.804508 | 2.088722e-49 | 1.078848e-46 |
| MsG0280007085.01 | MsG0580026282.01 | 0.811843 | 5.717017e-51 | 3.576891e-48 |
| MsG0280007653.01 | MsG0580026282.01 | -0.802445 | 5.592452e-49 | 2.739948e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026282.01.T01 | MTR_5g043210 | 90.972 | 144 | 5 | 1 | 1 | 136 | 120 | 263 | 7.45e-91 | 264 |
| MsG0580026282.01.T01 | MTR_5g043210 | 90.972 | 144 | 5 | 1 | 1 | 136 | 175 | 318 | 1.49e-90 | 265 |
| MsG0580026282.01.T01 | MTR_6g013425 | 58.741 | 143 | 51 | 1 | 2 | 136 | 174 | 316 | 6.22e-55 | 174 |
| MsG0580026282.01.T01 | MTR_1g013820 | 51.748 | 143 | 60 | 2 | 2 | 136 | 113 | 254 | 1.06e-44 | 146 |
| MsG0580026282.01.T01 | MTR_1g013820 | 51.748 | 143 | 60 | 2 | 2 | 136 | 111 | 252 | 1.16e-44 | 146 |
| MsG0580026282.01.T01 | MTR_1g013820 | 51.748 | 143 | 60 | 2 | 2 | 136 | 133 | 274 | 1.27e-44 | 147 |
| MsG0580026282.01.T01 | MTR_3g106000 | 51.748 | 143 | 58 | 3 | 2 | 136 | 135 | 274 | 3.30e-40 | 135 |
| MsG0580026282.01.T01 | MTR_8g442800 | 50.000 | 54 | 27 | 0 | 53 | 106 | 14 | 67 | 3.26e-11 | 55.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026282.01.T01 | AT1G69370 | 65.734 | 143 | 41 | 1 | 2 | 136 | 174 | 316 | 6.05e-62 | 192 |
| MsG0580026282.01.T01 | AT3G29200 | 60.667 | 150 | 44 | 2 | 2 | 136 | 191 | 340 | 4.13e-57 | 181 |
| MsG0580026282.01.T01 | AT5G10870 | 56.944 | 144 | 53 | 2 | 2 | 136 | 122 | 265 | 9.61e-50 | 160 |
Find 36 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAATATCTTTCGTTATTTA+AGG | 0.063416 | 5:-33924003 | MsG0580026282.01.T01:three_prime_UTR |
| GGTTTGATTGGCAATCTTTA+TGG | 0.174323 | 5:-33924316 | MsG0580026282.01.T01:CDS |
| AATGCTTACTTTCGCTTTAA+TGG | 0.176834 | 5:+33924572 | None:intergenic |
| TGCAAGATAATTTAGATGTT+TGG | 0.241997 | 5:-33924031 | MsG0580026282.01.T01:three_prime_UTR |
| GCAAGATAATTTAGATGTTT+GGG | 0.260432 | 5:-33924030 | MsG0580026282.01.T01:three_prime_UTR |
| TCACATCAAGTTCTTAGAAT+TGG | 0.291022 | 5:+33924176 | None:intergenic |
| TGCAAGTACTCAAGAACTTT+AGG | 0.327748 | 5:-33924145 | MsG0580026282.01.T01:three_prime_UTR |
| TCAATATAAACAAAATGATT+TGG | 0.330892 | 5:-33925112 | None:intergenic |
| AAAATCAAGTCAGGTTTGAT+TGG | 0.354826 | 5:-33924328 | MsG0580026282.01.T01:CDS |
| TAAGGATCTCCTTCCAAGAT+TGG | 0.357989 | 5:-33925078 | MsG0580026282.01.T01:CDS |
| TTGGCAATCTTTATGGTGAA+TGG | 0.422641 | 5:-33924309 | MsG0580026282.01.T01:CDS |
| TTTAATCAAGTCTTCGCAAC+AGG | 0.425055 | 5:+33924251 | None:intergenic |
| CTCTCAAAGAGAATCCACTA+TGG | 0.434884 | 5:-33924648 | MsG0580026282.01.T01:CDS |
| ACAGGACAGGAAACGGCTAC+TGG | 0.470469 | 5:-33924470 | MsG0580026282.01.T01:intron |
| TTCTCCTCCACACAAGAAAC+AGG | 0.472132 | 5:+33924105 | None:intergenic |
| CCTGTATATAAAATCAAGTC+AGG | 0.484671 | 5:-33924337 | MsG0580026282.01.T01:CDS |
| TTCACCTGTTTCTTGTGTGG+AGG | 0.494284 | 5:-33924109 | MsG0580026282.01.T01:three_prime_UTR |
| TTGGTAAAAGCAGGAGATGA+TGG | 0.500955 | 5:-33925059 | MsG0580026282.01.T01:CDS |
| AGAATGCCCTTCTGTATATG+AGG | 0.503112 | 5:-33924598 | MsG0580026282.01.T01:CDS |
| ATGATTCACAGGACAGGAAA+CGG | 0.519147 | 5:-33924477 | MsG0580026282.01.T01:intron |
| ACCTGCACTTCTTTCGTGAG+AGG | 0.526046 | 5:+33924280 | None:intergenic |
| GCCTCTCACGAAAGAAGTGC+AGG | 0.528449 | 5:-33924281 | MsG0580026282.01.T01:CDS |
| GCAGAAGAGAGTAGAAATGA+AGG | 0.532582 | 5:-33924413 | MsG0580026282.01.T01:CDS |
| CTTCCAAGATTGGTAAAAGC+AGG | 0.534609 | 5:-33925068 | MsG0580026282.01.T01:CDS |
| CTATGGAAAATTTGTTGCTG+AGG | 0.538176 | 5:-33924631 | MsG0580026282.01.T01:CDS |
| GTGGTAAAGATCAATGAAGC+AGG | 0.562259 | 5:-33924373 | MsG0580026282.01.T01:CDS |
| TGTGTGGAGGAGAATGATGA+AGG | 0.571401 | 5:-33924096 | MsG0580026282.01.T01:three_prime_UTR |
| CAAGATAATTTAGATGTTTG+GGG | 0.574388 | 5:-33924029 | MsG0580026282.01.T01:three_prime_UTR |
| GGCAAGAACATTCAGTCAAG+TGG | 0.590032 | 5:-33924392 | MsG0580026282.01.T01:CDS |
| ATGGCAGCCTCATATACAGA+AGG | 0.592543 | 5:+33924591 | None:intergenic |
| GTTGTTGACATATGAATCAG+TGG | 0.647423 | 5:-33924446 | MsG0580026282.01.T01:CDS |
| TGGCAGCCTCATATACAGAA+GGG | 0.649292 | 5:+33924592 | None:intergenic |
| GCAGGAGATGATGGTAACAG+TGG | 0.655534 | 5:-33925050 | MsG0580026282.01.T01:CDS |
| GTGTGGAGGAGAATGATGAA+GGG | 0.671377 | 5:-33924095 | MsG0580026282.01.T01:three_prime_UTR |
| TGGTAACAGTGGATCAGCTG+CGG | 0.692102 | 5:-33925039 | MsG0580026282.01.T01:intron |
| GTTGACATATGAATCAGTGG+AGG | 0.696907 | 5:-33924443 | MsG0580026282.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GATATATTATTACTATTATA+CGG | - | Chr5:33924347-33924366 | MsG0580026282.01.T01:CDS | 10.0% |
| !! | TTATAAATTATAATCATTAC+AGG | - | Chr5:33924403-33924422 | MsG0580026282.01.T01:CDS | 10.0% |
| !! | TTATAATTTATAACAAAACA+CGG | + | Chr5:33924396-33924415 | None:intergenic | 10.0% |
| !! | CTACAAAATTTATCTTGATA+TGG | - | Chr5:33924570-33924589 | MsG0580026282.01.T01:intron | 20.0% |
| !! | TCAATATCTTTCGTTATTTA+AGG | - | Chr5:33925073-33925092 | MsG0580026282.01.T01:CDS | 20.0% |
| !!! | GATTTGGAATATGTATTTTA+AGG | - | Chr5:33923980-33923999 | MsG0580026282.01.T01:three_prime_UTR | 20.0% |
| !!! | TTATCATTTTCAACAAAGTT+AGG | - | Chr5:33924118-33924137 | MsG0580026282.01.T01:three_prime_UTR | 20.0% |
| ! | AGTACTCAAAATGTCAATAT+AGG | + | Chr5:33924230-33924249 | None:intergenic | 25.0% |
| ! | ATAATCTCATGCAAAGTAAA+AGG | + | Chr5:33924268-33924287 | None:intergenic | 25.0% |
| ! | CAAGATAATTTAGATGTTTG+GGG | - | Chr5:33925047-33925066 | MsG0580026282.01.T01:CDS | 25.0% |
| ! | GACATAGGTAGTATATTATA+AGG | + | Chr5:33924536-33924555 | None:intergenic | 25.0% |
| ! | GCAAGATAATTTAGATGTTT+GGG | - | Chr5:33925046-33925065 | MsG0580026282.01.T01:CDS | 25.0% |
| ! | TAAAATATGTACTGTAACAC+TGG | + | Chr5:33924101-33924120 | None:intergenic | 25.0% |
| ! | TGCAAGATAATTTAGATGTT+TGG | - | Chr5:33925045-33925064 | MsG0580026282.01.T01:CDS | 25.0% |
| !! | TTTTGTAGTGTTCCAATATA+AGG | + | Chr5:33924558-33924577 | None:intergenic | 25.0% |
| AAAATCAAGTCAGGTTTGAT+TGG | - | Chr5:33924748-33924767 | MsG0580026282.01.T01:intron | 30.0% | |
| AATGCTTACTTTCGCTTTAA+TGG | + | Chr5:33924507-33924526 | None:intergenic | 30.0% | |
| CCTGTATATAAAATCAAGTC+AGG | - | Chr5:33924739-33924758 | MsG0580026282.01.T01:intron | 30.0% | |
| TACTACCTATGTCCTTATAT+TGG | - | Chr5:33924543-33924562 | MsG0580026282.01.T01:intron | 30.0% | |
| TCAATATAGGCAAAGAAATG+AGG | + | Chr5:33924217-33924236 | None:intergenic | 30.0% | |
| TCACATCAAGTTCTTAGAAT+TGG | + | Chr5:33924903-33924922 | None:intergenic | 30.0% | |
| !! | CCTGACTTGATTTTATATAC+AGG | + | Chr5:33924742-33924761 | None:intergenic | 30.0% |
| AGTTAGGAGAAATTGATGTG+TGG | - | Chr5:33924134-33924153 | MsG0580026282.01.T01:three_prime_UTR | 35.0% | |
| GGTTTGATTGGCAATCTTTA+TGG | - | Chr5:33924760-33924779 | MsG0580026282.01.T01:intron | 35.0% | |
| GTGTTCCAATATAAGGACAT+AGG | + | Chr5:33924551-33924570 | None:intergenic | 35.0% | |
| GTTGTTGACATATGAATCAG+TGG | - | Chr5:33924630-33924649 | MsG0580026282.01.T01:CDS | 35.0% | |
| TATGGCTTATCATGATTCAC+AGG | - | Chr5:33924588-33924607 | MsG0580026282.01.T01:CDS | 35.0% | |
| TGCAAGTACTCAAGAACTTT+AGG | - | Chr5:33924931-33924950 | MsG0580026282.01.T01:intron | 35.0% | |
| TGTAACACTGGAAGCTTAAT+AGG | + | Chr5:33924089-33924108 | None:intergenic | 35.0% | |
| TTGGCAATCTTTATGGTGAA+TGG | - | Chr5:33924767-33924786 | MsG0580026282.01.T01:intron | 35.0% | |
| TTTAATCAAGTCTTCGCAAC+AGG | + | Chr5:33924828-33924847 | None:intergenic | 35.0% | |
| ! | CAGCAACAAATTTTCCATAG+TGG | + | Chr5:33924445-33924464 | None:intergenic | 35.0% |
| ! | CATTTTGTTCCTACACCATA+TGG | + | Chr5:33924190-33924209 | None:intergenic | 35.0% |
| !! | CTATGGAAAATTTGTTGCTG+AGG | - | Chr5:33924445-33924464 | MsG0580026282.01.T01:CDS | 35.0% |
| !! | TTTTTCACCTGTTTCTTGTG+TGG | - | Chr5:33924964-33924983 | MsG0580026282.01.T01:intron | 35.0% |
| AGAATGCCCTTCTGTATATG+AGG | - | Chr5:33924478-33924497 | MsG0580026282.01.T01:intron | 40.0% | |
| ATGATTCACAGGACAGGAAA+CGG | - | Chr5:33924599-33924618 | MsG0580026282.01.T01:CDS | 40.0% | |
| CTCTCAAAGAGAATCCACTA+TGG | - | Chr5:33924428-33924447 | MsG0580026282.01.T01:CDS | 40.0% | |
| CTTATCATGATTCACAGGAC+AGG | - | Chr5:33924593-33924612 | MsG0580026282.01.T01:CDS | 40.0% | |
| CTTCCAAGATTGGTAAAAGC+AGG | - | Chr5:33924008-33924027 | MsG0580026282.01.T01:three_prime_UTR | 40.0% | |
| GCAGAAGAGAGTAGAAATGA+AGG | - | Chr5:33924663-33924682 | MsG0580026282.01.T01:intron | 40.0% | |
| GTGGTAAAGATCAATGAAGC+AGG | - | Chr5:33924703-33924722 | MsG0580026282.01.T01:intron | 40.0% | |
| GTTGACATATGAATCAGTGG+AGG | - | Chr5:33924633-33924652 | MsG0580026282.01.T01:CDS | 40.0% | |
| TAAGGATCTCCTTCCAAGAT+TGG | - | Chr5:33923998-33924017 | MsG0580026282.01.T01:three_prime_UTR | 40.0% | |
| TTGGTAAAAGCAGGAGATGA+TGG | - | Chr5:33924017-33924036 | MsG0580026282.01.T01:three_prime_UTR | 40.0% | |
| !! | CTGCTTTTACCAATCTTGGA+AGG | + | Chr5:33924010-33924029 | None:intergenic | 40.0% |
| !! | TCTCCTGCTTTTACCAATCT+TGG | + | Chr5:33924014-33924033 | None:intergenic | 40.0% |
| AACAGTGAAATCCACCCTAG+AGG | - | Chr5:33924162-33924181 | MsG0580026282.01.T01:three_prime_UTR | 45.0% | |
| ATGGCAGCCTCATATACAGA+AGG | + | Chr5:33924488-33924507 | None:intergenic | 45.0% | |
| GGCAAGAACATTCAGTCAAG+TGG | - | Chr5:33924684-33924703 | MsG0580026282.01.T01:intron | 45.0% | |
| GTGTGGAGGAGAATGATGAA+GGG | - | Chr5:33924981-33925000 | MsG0580026282.01.T01:intron | 45.0% | |
| TACACCATATGGAGCCTCTA+GGG | + | Chr5:33924179-33924198 | None:intergenic | 45.0% | |
| TGGCAGCCTCATATACAGAA+GGG | + | Chr5:33924487-33924506 | None:intergenic | 45.0% | |
| TGTGTGGAGGAGAATGATGA+AGG | - | Chr5:33924980-33924999 | MsG0580026282.01.T01:intron | 45.0% | |
| TTCTCCTCCACACAAGAAAC+AGG | + | Chr5:33924974-33924993 | None:intergenic | 45.0% | |
| ! | TTCACCTGTTTCTTGTGTGG+AGG | - | Chr5:33924967-33924986 | MsG0580026282.01.T01:intron | 45.0% |
| ACCTGCACTTCTTTCGTGAG+AGG | + | Chr5:33924799-33924818 | None:intergenic | 50.0% | |
| CTACACCATATGGAGCCTCT+AGG | + | Chr5:33924180-33924199 | None:intergenic | 50.0% | |
| CTAGAGGCTCCATATGGTGT+AGG | - | Chr5:33924178-33924197 | MsG0580026282.01.T01:three_prime_UTR | 50.0% | |
| CTGTGACACTCTTTGCTTGC+AGG | - | Chr5:33924061-33924080 | MsG0580026282.01.T01:three_prime_UTR | 50.0% | |
| TGGTAACAGTGGATCAGCTG+CGG | - | Chr5:33924037-33924056 | MsG0580026282.01.T01:three_prime_UTR | 50.0% | |
| ! | GCAGGAGATGATGGTAACAG+TGG | - | Chr5:33924026-33924045 | MsG0580026282.01.T01:three_prime_UTR | 50.0% |
| ACAGGACAGGAAACGGCTAC+TGG | - | Chr5:33924606-33924625 | MsG0580026282.01.T01:CDS | 55.0% | |
| TCCACCCTAGAGGCTCCATA+TGG | - | Chr5:33924172-33924191 | MsG0580026282.01.T01:three_prime_UTR | 55.0% | |
| ! | ACCATATGGAGCCTCTAGGG+TGG | + | Chr5:33924176-33924195 | None:intergenic | 55.0% |
| ! | GCCTCTCACGAAAGAAGTGC+AGG | - | Chr5:33924795-33924814 | MsG0580026282.01.T01:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 33923978 | 33925120 | 33923978 | ID=MsG0580026282.01;Name=MsG0580026282.01 |
| Chr5 | mRNA | 33923978 | 33925120 | 33923978 | ID=MsG0580026282.01.T01;Parent=MsG0580026282.01;Name=MsG0580026282.01.T01;_AED=0.28;_eAED=0.28;_QI=0|0.5|0.66|1|1|1|3|274|136 |
| Chr5 | exon | 33923978 | 33924488 | 33923978 | ID=MsG0580026282.01.T01:exon:25429;Parent=MsG0580026282.01.T01 |
| Chr5 | exon | 33924581 | 33924673 | 33924581 | ID=MsG0580026282.01.T01:exon:25428;Parent=MsG0580026282.01.T01 |
| Chr5 | exon | 33925040 | 33925120 | 33925040 | ID=MsG0580026282.01.T01:exon:25427;Parent=MsG0580026282.01.T01 |
| Chr5 | CDS | 33925040 | 33925120 | 33925040 | ID=MsG0580026282.01.T01:cds;Parent=MsG0580026282.01.T01 |
| Chr5 | CDS | 33924581 | 33924673 | 33924581 | ID=MsG0580026282.01.T01:cds;Parent=MsG0580026282.01.T01 |
| Chr5 | CDS | 33924252 | 33924488 | 33924252 | ID=MsG0580026282.01.T01:cds;Parent=MsG0580026282.01.T01 |
| Chr5 | three_prime_UTR | 33923978 | 33924251 | 33923978 | ID=MsG0580026282.01.T01:three_prime_utr;Parent=MsG0580026282.01.T01 |
| Gene Sequence |
| Protein sequence |
