Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003174.01 | MsG0780035989.01 | -0.822774 | 1.978258e-53 | 1.671796e-50 |
| MsG0180003233.01 | MsG0780035989.01 | 0.819739 | 9.915796e-53 | 7.694994e-50 |
| MsG0180005676.01 | MsG0780035989.01 | 0.800044 | 1.733041e-48 | 7.989193e-46 |
| MsG0380012661.01 | MsG0780035989.01 | 0.813862 | 2.065129e-51 | 1.364184e-48 |
| MsG0680031446.01 | MsG0780035989.01 | 0.803989 | 2.678726e-49 | 1.365399e-46 |
| MsG0480019163.01 | MsG0780035989.01 | 0.832218 | 1.071671e-55 | 1.189983e-52 |
| MsG0480021490.01 | MsG0780035989.01 | 0.803900 | 2.796122e-49 | 1.421947e-46 |
| MsG0480021776.01 | MsG0780035989.01 | 0.800299 | 1.537956e-48 | 7.136332e-46 |
| MsG0580024191.01 | MsG0780035989.01 | -0.819041 | 1.430350e-52 | 1.088709e-49 |
| MsG0380015598.01 | MsG0780035989.01 | 0.800456 | 1.429120e-48 | 6.657292e-46 |
| MsG0380016828.01 | MsG0780035989.01 | 0.808993 | 2.357048e-50 | 1.367505e-47 |
| MsG0280010423.01 | MsG0780035989.01 | 0.816472 | 5.433943e-52 | 3.853879e-49 |
| MsG0680035659.01 | MsG0780035989.01 | -0.804386 | 2.215201e-49 | 1.140616e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 14 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTTAGACTTGAAATCTAT+TGG | 0.263751 | 7:-992989 | MsG0780035989.01.T01:CDS |
| CCACCTTGTGTTTACCTAAC+AGG | 0.315388 | 7:+992906 | None:intergenic |
| CGAATGAGGCGATCTATAAC+GGG | 0.424891 | 7:+993034 | None:intergenic |
| ACGAATGAGGCGATCTATAA+CGG | 0.438700 | 7:+993033 | None:intergenic |
| CACCACATGGTAAATTCTCA+TGG | 0.457373 | 7:-992869 | MsG0780035989.01.T01:CDS |
| CAATTGATTTGAAACGAATG+AGG | 0.534268 | 7:+993020 | None:intergenic |
| ACCACATGGTAAATTCTCAT+GGG | 0.575766 | 7:-992868 | MsG0780035989.01.T01:CDS |
| CATTGATGCTTATCACCACA+TGG | 0.607402 | 7:-992882 | MsG0780035989.01.T01:CDS |
| AATGAGGCGATCTATAACGG+GGG | 0.630919 | 7:+993036 | None:intergenic |
| GTTCCTGTTAGGTAAACACA+AGG | 0.658024 | 7:-992909 | MsG0780035989.01.T01:CDS |
| CCTGTTAGGTAAACACAAGG+TGG | 0.719719 | 7:-992906 | MsG0780035989.01.T01:CDS |
| GAATGAGGCGATCTATAACG+GGG | 0.721477 | 7:+993035 | None:intergenic |
| ACCCATGAGAATTTACCATG+TGG | 0.744106 | 7:+992867 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CTCTTAGACTTGAAATCTAT+TGG | - | Chr7:992898-992917 | MsG0780035989.01.T01:CDS | 30.0% | |
| ! | TTAAGACTTTTGTTCCTGTT+AGG | - | Chr7:992967-992986 | MsG0780035989.01.T01:CDS | 30.0% |
| !! | CAATTGATTTGAAACGAATG+AGG | + | Chr7:992870-992889 | None:intergenic | 30.0% |
| ACCACATGGTAAATTCTCAT+GGG | - | Chr7:993019-993038 | MsG0780035989.01.T01:CDS | 35.0% | |
| ACCCATGAGAATTTACCATG+TGG | + | Chr7:993023-993042 | None:intergenic | 40.0% | |
| ACGAATGAGGCGATCTATAA+CGG | + | Chr7:992857-992876 | None:intergenic | 40.0% | |
| CACCACATGGTAAATTCTCA+TGG | - | Chr7:993018-993037 | MsG0780035989.01.T01:CDS | 40.0% | |
| CATTGATGCTTATCACCACA+TGG | - | Chr7:993005-993024 | MsG0780035989.01.T01:CDS | 40.0% | |
| GTTCCTGTTAGGTAAACACA+AGG | - | Chr7:992978-992997 | MsG0780035989.01.T01:CDS | 40.0% | |
| AATGAGGCGATCTATAACGG+GGG | + | Chr7:992854-992873 | None:intergenic | 45.0% | |
| CCACCTTGTGTTTACCTAAC+AGG | + | Chr7:992984-993003 | None:intergenic | 45.0% | |
| CCTGTTAGGTAAACACAAGG+TGG | - | Chr7:992981-993000 | MsG0780035989.01.T01:CDS | 45.0% | |
| CGAATGAGGCGATCTATAAC+GGG | + | Chr7:992856-992875 | None:intergenic | 45.0% | |
| GAATGAGGCGATCTATAACG+GGG | + | Chr7:992855-992874 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 992847 | 993062 | 992847 | ID=MsG0780035989.01;Name=MsG0780035989.01 |
| Chr7 | mRNA | 992847 | 993062 | 992847 | ID=MsG0780035989.01.T01;Parent=MsG0780035989.01;Name=MsG0780035989.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|71 |
| Chr7 | exon | 992847 | 993062 | 992847 | ID=MsG0780035989.01.T01:exon:15592;Parent=MsG0780035989.01.T01 |
| Chr7 | CDS | 992847 | 993062 | 992847 | ID=MsG0780035989.01.T01:cds;Parent=MsG0780035989.01.T01 |
| Gene Sequence |
| Protein sequence |
