AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780036946.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780036946.01.T01 MTR_7g024300 96.996 233 7 0 1 233 1 233 3.43e-168 463
MsG0780036946.01.T01 MTR_7g024280 79.736 227 41 2 1 223 1 226 4.01e-124 352
MsG0780036946.01.T01 MTR_2g013460 48.750 240 115 3 1 233 1 239 2.19e-72 221
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780036946.01.T01 AT1G65520 60.274 219 86 1 1 218 1 219 9.33e-95 277
MsG0780036946.01.T01 AT4G14440 49.576 236 113 3 1 231 1 235 3.67e-77 233
MsG0780036946.01.T01 AT4G14430 51.316 228 101 3 1 218 1 228 4.62e-65 202

Find 77 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTAAGGCTGCGGTTGAAT+TGG 0.323596 7:+17001837 MsG0780036946.01.T01:CDS
GGCGAAGGAGGCGGTGAGAT+TGG 0.327901 7:+17001777 MsG0780036946.01.T01:CDS
GCGAAGGAGGCGGTGAGATT+GGG 0.341668 7:+17001778 MsG0780036946.01.T01:CDS
CATCGTTGCCGGTTAGTGTT+AGG 0.344736 7:-17001318 None:intergenic
GAGAAGGTGTTCATCGTTGC+CGG 0.369830 7:-17001329 None:intergenic
TGACATTGATCATGTGATTC+CGG 0.384546 7:+17001672 MsG0780036946.01.T01:CDS
ATCTTTATCCTAACACTAAC+CGG 0.403226 7:+17001310 MsG0780036946.01.T01:CDS
GTTAAGGCTGCGGTTGAATT+GGG 0.413938 7:+17001838 MsG0780036946.01.T01:CDS
TGTCATGTGGTTAAGGCTGT+TGG 0.423767 7:+17001925 MsG0780036946.01.T01:CDS
GGTTGAATTGGGTGGTGATT+TGG 0.435908 7:+17001849 MsG0780036946.01.T01:CDS
ACCTGCGGCGGAGGCGTGAC+CGG 0.441572 7:-17001578 None:intergenic
ACCGGTCACGCCTCCGCCGC+AGG 0.460516 7:+17001577 MsG0780036946.01.T01:CDS
TAGCGTTGAGGAAACGGTTA+AGG 0.475993 7:+17001822 MsG0780036946.01.T01:CDS
GCATGATAGCGTTGAGGAAA+CGG 0.485014 7:+17001816 MsG0780036946.01.T01:CDS
TGTGATGAGGACGGAGGAGG+TGG 0.486253 7:-17001410 None:intergenic
CATAGGAAGAGAAATGAGTT+CGG 0.489925 7:-17001536 None:intergenic
GAGGAGGAAGATTGTGATGC+AGG 0.494034 7:+17001741 MsG0780036946.01.T01:CDS
TTGATTGAATTGAGAAGTGT+GGG 0.494586 7:-17001355 None:intergenic
GATTGTGATGCAGGCGGCGA+AGG 0.500935 7:+17001750 MsG0780036946.01.T01:CDS
GATGAGGACGGAGGAGGTGG+TGG 0.512390 7:-17001407 None:intergenic
CTTGTTGACGGACACGGTGG+AGG 0.515031 7:-17001384 None:intergenic
GAAGGTGACGGCGAAGGAGG+CGG 0.522620 7:+17001768 MsG0780036946.01.T01:CDS
CCTACCATCGCCGCTGTCAC+CGG 0.529761 7:+17001559 MsG0780036946.01.T01:CDS
TGAGGAAACGGTTAAGGCTG+CGG 0.530694 7:+17001828 MsG0780036946.01.T01:CDS
CGAAGGAGGCGGTGAGATTG+GGG 0.532153 7:+17001779 MsG0780036946.01.T01:CDS
AGGAGGTGGTGGCTTGTTGA+CGG 0.534669 7:-17001396 None:intergenic
GGCGAAGGTGACGGCGAAGG+AGG 0.537538 7:+17001765 MsG0780036946.01.T01:CDS
TTGTTGACGGACACGGTGGA+GGG 0.539713 7:-17001383 None:intergenic
GAGATTGGGGATTGTTGACT+CGG 0.544829 7:+17001792 MsG0780036946.01.T01:CDS
CCAACGGCTACGACATCGAC+TGG 0.545373 7:+17001455 MsG0780036946.01.T01:CDS
GACACGTGTATGCTGAGAAT+AGG 0.547375 7:+17001890 MsG0780036946.01.T01:CDS
GAGGATGTAACCTGCGGCGG+AGG 0.551154 7:-17001587 None:intergenic
GATGCAGGCGGCGAAGGTGA+CGG 0.558544 7:+17001756 MsG0780036946.01.T01:CDS
CCGGTGACAGCGGCGATGGT+AGG 0.559630 7:-17001559 None:intergenic
TGAGAAGTGTGGGGTTGAGA+AGG 0.560081 7:-17001345 None:intergenic
TTGGTGAAACGTGGATGGAA+TGG 0.560238 7:+17001868 MsG0780036946.01.T01:CDS
GCGATTGTTGATGCGAAGGT+TGG 0.567353 7:+17001706 MsG0780036946.01.T01:CDS
TTTGATTGAATTGAGAAGTG+TGG 0.568432 7:-17001356 None:intergenic
ATGTGCAGTTTAGAGAAGAA+AGG 0.573237 7:+17001283 MsG0780036946.01.T01:CDS
AGAAATGAGTTCGGAGACGA+CGG 0.573351 7:-17001527 None:intergenic
TGAGTTCGGAGACGACGGAA+CGG 0.575907 7:-17001522 None:intergenic
GTGACCGGTGACAGCGGCGA+TGG 0.580314 7:-17001563 None:intergenic
GGCGGCGAAGGTGACGGCGA+AGG 0.585170 7:+17001762 MsG0780036946.01.T01:CDS
AAGGCTGCGGTTGAATTGGG+TGG 0.589144 7:+17001841 MsG0780036946.01.T01:CDS
TGCCGTGTGCGGTTGTGATG+AGG 0.590140 7:-17001423 None:intergenic
TGGTGGCTTGTTGACGGACA+CGG 0.593982 7:-17001390 None:intergenic
GGTGGTGATTTGGTGAAACG+TGG 0.600174 7:+17001859 MsG0780036946.01.T01:CDS
CCAGTCGATGTCGTAGCCGT+TGG 0.601164 7:-17001455 None:intergenic
GTCCTCATCACAACCGCACA+CGG 0.601280 7:+17001421 MsG0780036946.01.T01:CDS
TTGACGGACACGGTGGAGGG+CGG 0.603626 7:-17001380 None:intergenic
TGTTGCGATTGTTGATGCGA+AGG 0.611424 7:+17001702 MsG0780036946.01.T01:CDS
GGTTGTGATGAGGACGGAGG+AGG 0.613000 7:-17001413 None:intergenic
GGAGAAGAATTTGCCGTGTG+CGG 0.613622 7:-17001434 None:intergenic
GTGATTTGGTGAAACGTGGA+TGG 0.616384 7:+17001863 MsG0780036946.01.T01:CDS
ACAGCGGCGATGGTAGGCAT+AGG 0.616579 7:-17001553 None:intergenic
CAACGGCTACGACATCGACT+GGG 0.618261 7:+17001456 MsG0780036946.01.T01:CDS
CTGTTGGAGATATTAGTGAG+AGG 0.626461 7:+17001941 MsG0780036946.01.T01:CDS
AGGTTGGTGATGCGGCGGCG+AGG 0.632976 7:+17001722 MsG0780036946.01.T01:CDS
TTGGTGATGCGGCGGCGAGG+AGG 0.634628 7:+17001725 MsG0780036946.01.T01:CDS
TGATGCGAAGGTTGGTGATG+CGG 0.635776 7:+17001714 MsG0780036946.01.T01:CDS
TGCGAGGATGTAACCTGCGG+CGG 0.635893 7:-17001590 None:intergenic
GGAGGCGTGACCGGTGACAG+CGG 0.649696 7:-17001569 None:intergenic
CACGGCAAATTCTTCTCCAA+CGG 0.650454 7:+17001439 MsG0780036946.01.T01:CDS
CATGTAGAGAAATCCACGAT+CGG 0.652552 7:-17001641 None:intergenic
AACGGAGGAGATCGTCGATG+AGG 0.661003 7:-17001504 None:intergenic
TTGATCATGTGATTCCGGCG+TGG 0.665310 7:+17001677 MsG0780036946.01.T01:CDS
TGCGAAGGTTGGTGATGCGG+CGG 0.665575 7:+17001717 MsG0780036946.01.T01:CDS
GAGGAAGATTGTGATGCAGG+CGG 0.666170 7:+17001744 MsG0780036946.01.T01:CDS
CTCGGCGCATGATAGCGTTG+AGG 0.673294 7:+17001810 MsG0780036946.01.T01:CDS
CATAGTCGTGAGATAATGCG+AGG 0.673407 7:-17001606 None:intergenic
GTTCGGAGACGACGGAACGG+AGG 0.680699 7:-17001519 None:intergenic
TGATTGAATTGAGAAGTGTG+GGG 0.704818 7:-17001354 None:intergenic
TGGCTTGTTGACGGACACGG+TGG 0.713580 7:-17001387 None:intergenic
TAATGCGAGGATGTAACCTG+CGG 0.724825 7:-17001593 None:intergenic
GTGTGCGGTTGTGATGAGGA+CGG 0.730224 7:-17001419 None:intergenic
ACAATCGCAACAAACCACGC+CGG 0.732102 7:-17001691 None:intergenic
TGCGGTTGTGATGAGGACGG+AGG 0.757505 7:-17001416 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATCTTTATCCTAACACTAAC+CGG + Chr7:17001310-17001329 MsG0780036946.01.T01:CDS 30.0%
TTTGATTGAATTGAGAAGTG+TGG - Chr7:17001359-17001378 None:intergenic 30.0%
! TTGATTGAATTGAGAAGTGT+GGG - Chr7:17001358-17001377 None:intergenic 30.0%
ATGTGCAGTTTAGAGAAGAA+AGG + Chr7:17001283-17001302 MsG0780036946.01.T01:CDS 35.0%
CATAGGAAGAGAAATGAGTT+CGG - Chr7:17001539-17001558 None:intergenic 35.0%
TGACATTGATCATGTGATTC+CGG + Chr7:17001672-17001691 MsG0780036946.01.T01:CDS 35.0%
! GAAGAAGTTTTTGTGTCATG+TGG + Chr7:17001912-17001931 MsG0780036946.01.T01:CDS 35.0%
! GTTTTTGTGTCATGTGGTTA+AGG + Chr7:17001918-17001937 MsG0780036946.01.T01:CDS 35.0%
! TGATTGAATTGAGAAGTGTG+GGG - Chr7:17001357-17001376 None:intergenic 35.0%
CATGTAGAGAAATCCACGAT+CGG - Chr7:17001644-17001663 None:intergenic 40.0%
CTGTTGGAGATATTAGTGAG+AGG + Chr7:17001941-17001960 MsG0780036946.01.T01:CDS 40.0%
AGAAATGAGTTCGGAGACGA+CGG - Chr7:17001530-17001549 None:intergenic 45.0%
CACGGCAAATTCTTCTCCAA+CGG + Chr7:17001439-17001458 MsG0780036946.01.T01:CDS 45.0%
CATAGTCGTGAGATAATGCG+AGG - Chr7:17001609-17001628 None:intergenic 45.0%
GACACGTGTATGCTGAGAAT+AGG + Chr7:17001890-17001909 MsG0780036946.01.T01:CDS 45.0%
GGTTGAATTGGGTGGTGATT+TGG + Chr7:17001849-17001868 MsG0780036946.01.T01:CDS 45.0%
GTTAAGGCTGCGGTTGAATT+GGG + Chr7:17001838-17001857 MsG0780036946.01.T01:CDS 45.0%
TAATGCGAGGATGTAACCTG+CGG - Chr7:17001596-17001615 None:intergenic 45.0%
TGTCATGTGGTTAAGGCTGT+TGG + Chr7:17001925-17001944 MsG0780036946.01.T01:CDS 45.0%
TGTTGCGATTGTTGATGCGA+AGG + Chr7:17001702-17001721 MsG0780036946.01.T01:CDS 45.0%
TTGGTGAAACGTGGATGGAA+TGG + Chr7:17001868-17001887 MsG0780036946.01.T01:CDS 45.0%
! GAGATTGGGGATTGTTGACT+CGG + Chr7:17001792-17001811 MsG0780036946.01.T01:CDS 45.0%
! GCATGATAGCGTTGAGGAAA+CGG + Chr7:17001816-17001835 MsG0780036946.01.T01:CDS 45.0%
! TAGCGTTGAGGAAACGGTTA+AGG + Chr7:17001822-17001841 MsG0780036946.01.T01:CDS 45.0%
!! GTGATTTGGTGAAACGTGGA+TGG + Chr7:17001863-17001882 MsG0780036946.01.T01:CDS 45.0%
!! GTTTTAATGCGATCCGATCG+TGG + Chr7:17001628-17001647 MsG0780036946.01.T01:CDS 45.0%
ACAATCGCAACAAACCACGC+CGG - Chr7:17001694-17001713 None:intergenic 50.0%
GAGGAAGATTGTGATGCAGG+CGG + Chr7:17001744-17001763 MsG0780036946.01.T01:CDS 50.0%
GAGGAGGAAGATTGTGATGC+AGG + Chr7:17001741-17001760 MsG0780036946.01.T01:CDS 50.0%
GCGATTGTTGATGCGAAGGT+TGG + Chr7:17001706-17001725 MsG0780036946.01.T01:CDS 50.0%
GGAGAAGAATTTGCCGTGTG+CGG - Chr7:17001437-17001456 None:intergenic 50.0%
GGTTAAGGCTGCGGTTGAAT+TGG + Chr7:17001837-17001856 MsG0780036946.01.T01:CDS 50.0%
TGAGGAAACGGTTAAGGCTG+CGG + Chr7:17001828-17001847 MsG0780036946.01.T01:CDS 50.0%
TTGATCATGTGATTCCGGCG+TGG + Chr7:17001677-17001696 MsG0780036946.01.T01:CDS 50.0%
! CATCGTTGCCGGTTAGTGTT+AGG - Chr7:17001321-17001340 None:intergenic 50.0%
! TGAGAAGTGTGGGGTTGAGA+AGG - Chr7:17001348-17001367 None:intergenic 50.0%
!! GAGAAGGTGTTCATCGTTGC+CGG - Chr7:17001332-17001351 None:intergenic 50.0%
!! GGTGGTGATTTGGTGAAACG+TGG + Chr7:17001859-17001878 MsG0780036946.01.T01:CDS 50.0%
!! TGATGCGAAGGTTGGTGATG+CGG + Chr7:17001714-17001733 MsG0780036946.01.T01:CDS 50.0%
AACGGAGGAGATCGTCGATG+AGG - Chr7:17001507-17001526 None:intergenic 55.0%
AAGGCTGCGGTTGAATTGGG+TGG + Chr7:17001841-17001860 MsG0780036946.01.T01:CDS 55.0%
GTCCTCATCACAACCGCACA+CGG + Chr7:17001421-17001440 MsG0780036946.01.T01:CDS 55.0%
GTGTGCGGTTGTGATGAGGA+CGG - Chr7:17001422-17001441 None:intergenic 55.0%
TGAGTTCGGAGACGACGGAA+CGG - Chr7:17001525-17001544 None:intergenic 55.0%
! CAACGGCTACGACATCGACT+GGG + Chr7:17001456-17001475 MsG0780036946.01.T01:CDS 55.0%
! TTGTTGACGGACACGGTGGA+GGG - Chr7:17001386-17001405 None:intergenic 55.0%
!! AGGAGGTGGTGGCTTGTTGA+CGG - Chr7:17001399-17001418 None:intergenic 55.0%
!! TGGTGGCTTGTTGACGGACA+CGG - Chr7:17001393-17001412 None:intergenic 55.0%
ACAGCGGCGATGGTAGGCAT+AGG - Chr7:17001556-17001575 None:intergenic 60.0%
CCAACGGCTACGACATCGAC+TGG + Chr7:17001455-17001474 MsG0780036946.01.T01:CDS 60.0%
CCAGTCGATGTCGTAGCCGT+TGG - Chr7:17001458-17001477 None:intergenic 60.0%
CGAAGGAGGCGGTGAGATTG+GGG + Chr7:17001779-17001798 MsG0780036946.01.T01:CDS 60.0%
GATTGTGATGCAGGCGGCGA+AGG + Chr7:17001750-17001769 MsG0780036946.01.T01:CDS 60.0%
GCGAAGGAGGCGGTGAGATT+GGG + Chr7:17001778-17001797 MsG0780036946.01.T01:CDS 60.0%
GGTTGTGATGAGGACGGAGG+AGG - Chr7:17001416-17001435 None:intergenic 60.0%
TGCCGTGTGCGGTTGTGATG+AGG - Chr7:17001426-17001445 None:intergenic 60.0%
TGCGAGGATGTAACCTGCGG+CGG - Chr7:17001593-17001612 None:intergenic 60.0%
TGCGGTTGTGATGAGGACGG+AGG - Chr7:17001419-17001438 None:intergenic 60.0%
TGTGATGAGGACGGAGGAGG+TGG - Chr7:17001413-17001432 None:intergenic 60.0%
! CTCGGCGCATGATAGCGTTG+AGG + Chr7:17001810-17001829 MsG0780036946.01.T01:CDS 60.0%
! CTTGTTGACGGACACGGTGG+AGG - Chr7:17001387-17001406 None:intergenic 60.0%
!! TGCGAAGGTTGGTGATGCGG+CGG + Chr7:17001717-17001736 MsG0780036946.01.T01:CDS 60.0%
!! TGGCTTGTTGACGGACACGG+TGG - Chr7:17001390-17001409 None:intergenic 60.0%
CCTACCATCGCCGCTGTCAC+CGG + Chr7:17001559-17001578 MsG0780036946.01.T01:CDS 65.0%
GAGGATGTAACCTGCGGCGG+AGG - Chr7:17001590-17001609 None:intergenic 65.0%
GATGAGGACGGAGGAGGTGG+TGG - Chr7:17001410-17001429 None:intergenic 65.0%
GATGCAGGCGGCGAAGGTGA+CGG + Chr7:17001756-17001775 MsG0780036946.01.T01:CDS 65.0%
GGCGAAGGAGGCGGTGAGAT+TGG + Chr7:17001777-17001796 MsG0780036946.01.T01:CDS 65.0%
GTTCGGAGACGACGGAACGG+AGG - Chr7:17001522-17001541 None:intergenic 65.0%
! GAAGGTGACGGCGAAGGAGG+CGG + Chr7:17001768-17001787 MsG0780036946.01.T01:CDS 65.0%
! TTGACGGACACGGTGGAGGG+CGG - Chr7:17001383-17001402 None:intergenic 65.0%
CCGGTGACAGCGGCGATGGT+AGG - Chr7:17001562-17001581 None:intergenic 70.0%
GTGACCGGTGACAGCGGCGA+TGG - Chr7:17001566-17001585 None:intergenic 70.0%
! GGAGGCGTGACCGGTGACAG+CGG - Chr7:17001572-17001591 None:intergenic 70.0%
! GGCGAAGGTGACGGCGAAGG+AGG + Chr7:17001765-17001784 MsG0780036946.01.T01:CDS 70.0%
!! AGGTTGGTGATGCGGCGGCG+AGG + Chr7:17001722-17001741 MsG0780036946.01.T01:CDS 70.0%
!! TTGGTGATGCGGCGGCGAGG+AGG + Chr7:17001725-17001744 MsG0780036946.01.T01:CDS 70.0%
! ACCTGCGGCGGAGGCGTGAC+CGG - Chr7:17001581-17001600 None:intergenic 75.0%
! GGCGGCGAAGGTGACGGCGA+AGG + Chr7:17001762-17001781 MsG0780036946.01.T01:CDS 75.0%
! ACCGGTCACGCCTCCGCCGC+AGG + Chr7:17001577-17001596 MsG0780036946.01.T01:CDS 80.0%
Chromosome Type Strat End Strand Name
Chr7 gene 17001283 17001984 17001283 ID=MsG0780036946.01;Name=MsG0780036946.01
Chr7 mRNA 17001283 17001984 17001283 ID=MsG0780036946.01.T01;Parent=MsG0780036946.01;Name=MsG0780036946.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|233
Chr7 exon 17001283 17001984 17001283 ID=MsG0780036946.01.T01:exon:2850;Parent=MsG0780036946.01.T01
Chr7 CDS 17001283 17001984 17001283 ID=MsG0780036946.01.T01:cds;Parent=MsG0780036946.01.T01
Gene Sequence

>MsG0780036946.01.T01

ATGTGCAGTTTAGAGAAGAAAGGCAACATCTTTATCCTAACACTAACCGGCAACGATGAACACCTTCTCAACCCCACACTTCTCAATTCAATCAAATCCGCCCTCCACCGTGTCCGTCAACAAGCCACCACCTCCTCCGTCCTCATCACAACCGCACACGGCAAATTCTTCTCCAACGGCTACGACATCGACTGGGCACAAGACAACAAATCACGCATCATCCTCATCGACGATCTCCTCCGTTCCGTCGTCTCCGAACTCATTTCTCTTCCTATGCCTACCATCGCCGCTGTCACCGGTCACGCCTCCGCCGCAGGTTACATCCTCGCATTATCTCACGACTATGTTTTAATGCGATCCGATCGTGGATTTCTCTACATGAGTGAACTTGACATTGATCATGTGATTCCGGCGTGGTTTGTTGCGATTGTTGATGCGAAGGTTGGTGATGCGGCGGCGAGGAGGAAGATTGTGATGCAGGCGGCGAAGGTGACGGCGAAGGAGGCGGTGAGATTGGGGATTGTTGACTCGGCGCATGATAGCGTTGAGGAAACGGTTAAGGCTGCGGTTGAATTGGGTGGTGATTTGGTGAAACGTGGATGGAATGGACACGTGTATGCTGAGAATAGGAAGAAGTTTTTGTGTCATGTGGTTAAGGCTGTTGGAGATATTAGTGAGAGGAATATTGAATCAAAACTTTAG

Protein sequence

>MsG0780036946.01.T01

MCSLEKKGNIFILTLTGNDEHLLNPTLLNSIKSALHRVRQQATTSSVLITTAHGKFFSNGYDIDWAQDNKSRIILIDDLLRSVVSELISLPMPTIAAVTGHASAAGYILALSHDYVLMRSDRGFLYMSELDIDHVIPAWFVAIVDAKVGDAAARRKIVMQAAKVTAKEAVRLGIVDSAHDSVEETVKAAVELGGDLVKRGWNGHVYAENRKKFLCHVVKAVGDISERNIESKL*