Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036981.01.T01 | XP_013447886.1 | 95.699 | 93 | 4 | 0 | 1 | 93 | 45 | 137 | 6.63E-58 | 184 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036981.01.T01 | A0A072TYJ6 | 95.699 | 93 | 4 | 0 | 1 | 93 | 45 | 137 | 3.17e-58 | 184 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0780036981.01 | MsG0780036982.01 | 0.837326 | 5.552808e-57 | 7.187920e-54 |
| MsG0780036981.01 | MsG0780036983.01 | 0.804570 | 2.027745e-49 | 1.049058e-46 |
| MsG0780036981.01 | MsG0780036984.01 | 0.831081 | 2.043343e-55 | 2.194064e-52 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036981.01.T01 | MTR_7g024873 | 95.699 | 93 | 4 | 0 | 1 | 93 | 45 | 137 | 8.03e-62 | 184 |
| MsG0780036981.01.T01 | MTR_7g024890 | 90.323 | 93 | 9 | 0 | 1 | 93 | 45 | 137 | 3.46e-58 | 175 |
| MsG0780036981.01.T01 | MTR_7g024880 | 88.172 | 93 | 11 | 0 | 1 | 93 | 45 | 137 | 5.19e-56 | 169 |
| MsG0780036981.01.T01 | MTR_8g446810 | 51.064 | 94 | 43 | 1 | 3 | 93 | 47 | 140 | 1.61e-27 | 97.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036981.01.T01 | AT2G44310 | 46.154 | 91 | 47 | 1 | 3 | 91 | 47 | 137 | 1.38e-23 | 87.8 |
Find 17 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAAACACGAGCAAGCTCATT+TGG | 0.308059 | 7:+17484224 | None:intergenic |
| TGAAAGAGAGTCCACTAAAT+TGG | 0.351854 | 7:-17484039 | MsG0780036981.01.T01:CDS |
| CTTGAATGGCACGGTTGATC+TGG | 0.357110 | 7:-17484171 | MsG0780036981.01.T01:CDS |
| TATTTAAGCAGCCAATTTAG+TGG | 0.374310 | 7:+17484028 | None:intergenic |
| CATGCCAATTCAAATGGTCT+TGG | 0.391639 | 7:-17484090 | MsG0780036981.01.T01:CDS |
| TCTTCCAAGACCATTTGAAT+TGG | 0.395041 | 7:+17484086 | None:intergenic |
| AAACACGAGCAAGCTCATTT+GGG | 0.456333 | 7:+17484225 | None:intergenic |
| GGGTTTCATGCCAATTCAAA+TGG | 0.458206 | 7:-17484096 | MsG0780036981.01.T01:CDS |
| CTCCTATGATGAGATGTTGA+AGG | 0.467641 | 7:-17484303 | None:intergenic |
| CAGTTCGATCACAACTTGAA+TGG | 0.477280 | 7:-17484185 | MsG0780036981.01.T01:CDS |
| GAAGGAGCTCAAGTGCTTGA+GGG | 0.496779 | 7:-17484285 | MsG0780036981.01.T01:CDS |
| GCTTGCAATGGCAGATGGAA+TGG | 0.514699 | 7:-17484117 | MsG0780036981.01.T01:CDS |
| CTTGCAATGGCAGATGGAAT+GGG | 0.535718 | 7:-17484116 | MsG0780036981.01.T01:CDS |
| TGAAGGAGCTCAAGTGCTTG+AGG | 0.550425 | 7:-17484286 | MsG0780036981.01.T01:CDS |
| ATGATGCTTGCAATGGCAGA+TGG | 0.563021 | 7:-17484122 | MsG0780036981.01.T01:CDS |
| TAAGCAAATGATGCTTGCAA+TGG | 0.606338 | 7:-17484129 | MsG0780036981.01.T01:CDS |
| CGATCACAACTTGAATGGCA+CGG | 0.606932 | 7:-17484180 | MsG0780036981.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CTCTTTCAACAGCTATTTTA+AGG | + | Chr7:17484270-17484289 | None:intergenic | 30.0% |
| TAAGCAAATGATGCTTGCAA+TGG | - | Chr7:17484192-17484211 | MsG0780036981.01.T01:CDS | 35.0% | |
| TCTTCCAAGACCATTTGAAT+TGG | + | Chr7:17484238-17484257 | None:intergenic | 35.0% | |
| TGAAAGAGAGTCCACTAAAT+TGG | - | Chr7:17484282-17484301 | MsG0780036981.01.T01:CDS | 35.0% | |
| !!! | AGGGTTTTTGAAACACACTT+TGG | - | Chr7:17484055-17484074 | MsG0780036981.01.T01:CDS | 35.0% |
| AAACACGAGCAAGCTCATTT+GGG | + | Chr7:17484099-17484118 | None:intergenic | 40.0% | |
| CAGTTCGATCACAACTTGAA+TGG | - | Chr7:17484136-17484155 | MsG0780036981.01.T01:CDS | 40.0% | |
| CATGCCAATTCAAATGGTCT+TGG | - | Chr7:17484231-17484250 | MsG0780036981.01.T01:CDS | 40.0% | |
| GGGTTTCATGCCAATTCAAA+TGG | - | Chr7:17484225-17484244 | MsG0780036981.01.T01:CDS | 40.0% | |
| TAAACACGAGCAAGCTCATT+TGG | + | Chr7:17484100-17484119 | None:intergenic | 40.0% | |
| ATGATGCTTGCAATGGCAGA+TGG | - | Chr7:17484199-17484218 | MsG0780036981.01.T01:CDS | 45.0% | |
| CGATCACAACTTGAATGGCA+CGG | - | Chr7:17484141-17484160 | MsG0780036981.01.T01:CDS | 45.0% | |
| CTTGCAATGGCAGATGGAAT+GGG | - | Chr7:17484205-17484224 | MsG0780036981.01.T01:CDS | 45.0% | |
| CTTGAATGGCACGGTTGATC+TGG | - | Chr7:17484150-17484169 | MsG0780036981.01.T01:CDS | 50.0% | |
| GAAGGAGCTCAAGTGCTTGA+GGG | - | Chr7:17484036-17484055 | MsG0780036981.01.T01:CDS | 50.0% | |
| GCTTGCAATGGCAGATGGAA+TGG | - | Chr7:17484204-17484223 | MsG0780036981.01.T01:CDS | 50.0% | |
| TGAAGGAGCTCAAGTGCTTG+AGG | - | Chr7:17484035-17484054 | MsG0780036981.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 17484031 | 17484312 | 17484031 | ID=MsG0780036981.01;Name=MsG0780036981.01 |
| Chr7 | mRNA | 17484031 | 17484312 | 17484031 | ID=MsG0780036981.01.T01;Parent=MsG0780036981.01;Name=MsG0780036981.01.T01;_AED=0.36;_eAED=0.36;_QI=0|-1|0|1|-1|1|1|0|93 |
| Chr7 | exon | 17484031 | 17484312 | 17484031 | ID=MsG0780036981.01.T01:exon:3283;Parent=MsG0780036981.01.T01 |
| Chr7 | CDS | 17484031 | 17484312 | 17484031 | ID=MsG0780036981.01.T01:cds;Parent=MsG0780036981.01.T01 |
| Gene Sequence |
| Protein sequence |
