Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036982.01.T01 | RHN44803.1 | 96.35 | 137 | 5 | 0 | 1 | 137 | 18 | 154 | 2.96E-90 | 268 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036982.01.T01 | A0A396GWC2 | 96.350 | 137 | 5 | 0 | 1 | 137 | 18 | 154 | 1.41e-90 | 268 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0780036981.01 | MsG0780036982.01 | 0.837326 | 5.552808e-57 | 7.187920e-54 |
| MsG0780036982.01 | MsG0780036983.01 | 0.873951 | 9.874676e-68 | 4.364724e-64 |
| MsG0780036982.01 | MsG0780036984.01 | 0.863588 | 2.235826e-64 | 6.813317e-61 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036982.01.T01 | MTR_7g024873 | 96.350 | 137 | 5 | 0 | 1 | 137 | 1 | 137 | 4.69e-94 | 268 |
| MsG0780036982.01.T01 | MTR_7g024890 | 91.971 | 137 | 11 | 0 | 1 | 137 | 1 | 137 | 2.53e-90 | 258 |
| MsG0780036982.01.T01 | MTR_7g024880 | 90.511 | 137 | 13 | 0 | 1 | 137 | 1 | 137 | 2.93e-88 | 253 |
| MsG0780036982.01.T01 | MTR_8g446810 | 49.286 | 140 | 68 | 1 | 1 | 137 | 1 | 140 | 1.48e-42 | 137 |
| MsG0780036982.01.T01 | MTR_7g024900 | 92.157 | 51 | 4 | 0 | 1 | 51 | 1 | 51 | 4.59e-27 | 95.9 |
| MsG0780036982.01.T01 | MTR_1g081270 | 35.878 | 131 | 79 | 1 | 1 | 131 | 1 | 126 | 1.06e-19 | 79.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036982.01.T01 | AT2G44310 | 49.635 | 137 | 67 | 1 | 1 | 135 | 1 | 137 | 4.05e-42 | 136 |
Find 30 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGATGAGTGTAGAAATTT+TGG | 0.159822 | 7:-17493988 | None:intergenic |
| CATTTAAGCAGCCAATTTAC+TGG | 0.249329 | 7:+17493590 | None:intergenic |
| CGGTTGATCTTGAAGAATTC+AGG | 0.278025 | 7:-17493722 | MsG0780036982.01.T01:CDS |
| TAAACACGAGCAAGCTCATT+TGG | 0.308059 | 7:+17493786 | None:intergenic |
| TGAAAGAGAGTCCAGTAAAT+TGG | 0.375239 | 7:-17493601 | MsG0780036982.01.T01:CDS |
| TAAATGCTTCTTCATCTTCA+AGG | 0.379890 | 7:+17493943 | None:intergenic |
| TCTTCCAACACCATTTGAAT+TGG | 0.382295 | 7:+17493648 | None:intergenic |
| CAGTTTGATCACAACTTGAA+TGG | 0.425360 | 7:-17493747 | MsG0780036982.01.T01:CDS |
| CTTCTTCATCTTCAAGGAAG+TGG | 0.447415 | 7:+17493949 | None:intergenic |
| TTAATAGCTCGGTAGGAAAT+CGG | 0.454217 | 7:-17493923 | MsG0780036982.01.T01:CDS |
| CTCCTTCAACATCTCATCAT+AGG | 0.454389 | 7:+17493863 | None:intergenic |
| AAACACGAGCAAGCTCATTT+GGG | 0.456333 | 7:+17493787 | None:intergenic |
| GGGTTTCATGCCAATTCAAA+TGG | 0.458206 | 7:-17493658 | MsG0780036982.01.T01:CDS |
| CTCCTATGATGAGATGTTGA+AGG | 0.467641 | 7:-17493865 | MsG0780036982.01.T01:CDS |
| CATGCCAATTCAAATGGTGT+TGG | 0.494072 | 7:-17493652 | MsG0780036982.01.T01:CDS |
| GAAGGAGCTCAAGAGCTTGA+GGG | 0.520375 | 7:-17493847 | MsG0780036982.01.T01:CDS |
| GCTTGCAATGGCAGATGGGA+TGG | 0.521128 | 7:-17493679 | MsG0780036982.01.T01:CDS |
| GAAGCATTTAATAGCTCGGT+AGG | 0.541215 | 7:-17493930 | MsG0780036982.01.T01:CDS |
| ATGATGCTTGCAATGGCAGA+TGG | 0.545635 | 7:-17493684 | MsG0780036982.01.T01:CDS |
| TGAAGAAGCATTTAATAGCT+CGG | 0.548389 | 7:-17493934 | MsG0780036982.01.T01:CDS |
| CTTGCAATGGCAGATGGGAT+GGG | 0.557101 | 7:-17493678 | MsG0780036982.01.T01:CDS |
| TGAAGGAGCTCAAGAGCTTG+AGG | 0.558625 | 7:-17493848 | MsG0780036982.01.T01:CDS |
| AACACACTTTGGTATTGATG+TGG | 0.581840 | 7:-17493817 | MsG0780036982.01.T01:CDS |
| GACCGTCGTTATTTGTGTCG+AGG | 0.583989 | 7:+17493892 | None:intergenic |
| TTCAAGGAAGTGGACAATTG+TGG | 0.596143 | 7:+17493959 | None:intergenic |
| TAAGCAAATGATGCTTGCAA+TGG | 0.606338 | 7:-17493691 | MsG0780036982.01.T01:CDS |
| TGATGCTTGCAATGGCAGAT+GGG | 0.619568 | 7:-17493683 | MsG0780036982.01.T01:CDS |
| TGATCACAACTTGAATGGCA+CGG | 0.635410 | 7:-17493742 | MsG0780036982.01.T01:CDS |
| TGATCTTGAAGAATTCAGGA+AGG | 0.643588 | 7:-17493718 | MsG0780036982.01.T01:CDS |
| TGCCTCGACACAAATAACGA+CGG | 0.718573 | 7:-17493894 | MsG0780036982.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TAAATGCTTCTTCATCTTCA+AGG | + | Chr7:17493637-17493656 | None:intergenic | 30.0% | |
| ! | ATGAGTGTAGAAATTTTGGA+TGG | - | Chr7:17493593-17493612 | MsG0780036982.01.T01:CDS | 30.0% |
| !! | TGAAGAAGCATTTAATAGCT+CGG | - | Chr7:17493643-17493662 | MsG0780036982.01.T01:CDS | 30.0% |
| CAGTTTGATCACAACTTGAA+TGG | - | Chr7:17493830-17493849 | MsG0780036982.01.T01:CDS | 35.0% | |
| TAAGCAAATGATGCTTGCAA+TGG | - | Chr7:17493886-17493905 | MsG0780036982.01.T01:CDS | 35.0% | |
| TCTTCCAACACCATTTGAAT+TGG | + | Chr7:17493932-17493951 | None:intergenic | 35.0% | |
| TGAAAGAGAGTCCAGTAAAT+TGG | - | Chr7:17493976-17493995 | MsG0780036982.01.T01:CDS | 35.0% | |
| TGATCTTGAAGAATTCAGGA+AGG | - | Chr7:17493859-17493878 | MsG0780036982.01.T01:CDS | 35.0% | |
| TTAATAGCTCGGTAGGAAAT+CGG | - | Chr7:17493654-17493673 | MsG0780036982.01.T01:CDS | 35.0% | |
| !! | AACACACTTTGGTATTGATG+TGG | - | Chr7:17493760-17493779 | MsG0780036982.01.T01:CDS | 35.0% |
| !! | CTCTTTCAACAGCTATTTTC+AGG | + | Chr7:17493964-17493983 | None:intergenic | 35.0% |
| !!! | AGGGTTTTTGAAACACACTT+TGG | - | Chr7:17493749-17493768 | MsG0780036982.01.T01:CDS | 35.0% |
| AAACACGAGCAAGCTCATTT+GGG | + | Chr7:17493793-17493812 | None:intergenic | 40.0% | |
| CATGCCAATTCAAATGGTGT+TGG | - | Chr7:17493925-17493944 | MsG0780036982.01.T01:CDS | 40.0% | |
| CGGTTGATCTTGAAGAATTC+AGG | - | Chr7:17493855-17493874 | MsG0780036982.01.T01:CDS | 40.0% | |
| CTCCTATGATGAGATGTTGA+AGG | - | Chr7:17493712-17493731 | MsG0780036982.01.T01:CDS | 40.0% | |
| CTCCTTCAACATCTCATCAT+AGG | + | Chr7:17493717-17493736 | None:intergenic | 40.0% | |
| CTTCTTCATCTTCAAGGAAG+TGG | + | Chr7:17493631-17493650 | None:intergenic | 40.0% | |
| GGGTTTCATGCCAATTCAAA+TGG | - | Chr7:17493919-17493938 | MsG0780036982.01.T01:CDS | 40.0% | |
| TAAACACGAGCAAGCTCATT+TGG | + | Chr7:17493794-17493813 | None:intergenic | 40.0% | |
| TGATCACAACTTGAATGGCA+CGG | - | Chr7:17493835-17493854 | MsG0780036982.01.T01:CDS | 40.0% | |
| TTCAAGGAAGTGGACAATTG+TGG | + | Chr7:17493621-17493640 | None:intergenic | 40.0% | |
| ! | GAAGCATTTAATAGCTCGGT+AGG | - | Chr7:17493647-17493666 | MsG0780036982.01.T01:CDS | 40.0% |
| ATGATGCTTGCAATGGCAGA+TGG | - | Chr7:17493893-17493912 | MsG0780036982.01.T01:CDS | 45.0% | |
| TGATGCTTGCAATGGCAGAT+GGG | - | Chr7:17493894-17493913 | MsG0780036982.01.T01:CDS | 45.0% | |
| TGCCTCGACACAAATAACGA+CGG | - | Chr7:17493683-17493702 | MsG0780036982.01.T01:CDS | 45.0% | |
| CTTGCAATGGCAGATGGGAT+GGG | - | Chr7:17493899-17493918 | MsG0780036982.01.T01:CDS | 50.0% | |
| GAAGGAGCTCAAGAGCTTGA+GGG | - | Chr7:17493730-17493749 | MsG0780036982.01.T01:CDS | 50.0% | |
| GACCGTCGTTATTTGTGTCG+AGG | + | Chr7:17493688-17493707 | None:intergenic | 50.0% | |
| TGAAGGAGCTCAAGAGCTTG+AGG | - | Chr7:17493729-17493748 | MsG0780036982.01.T01:CDS | 50.0% | |
| GCTTGCAATGGCAGATGGGA+TGG | - | Chr7:17493898-17493917 | MsG0780036982.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 17493593 | 17494006 | 17493593 | ID=MsG0780036982.01;Name=MsG0780036982.01 |
| Chr7 | mRNA | 17493593 | 17494006 | 17493593 | ID=MsG0780036982.01.T01;Parent=MsG0780036982.01;Name=MsG0780036982.01.T01;_AED=0.29;_eAED=0.29;_QI=0|-1|0|1|-1|1|1|0|137 |
| Chr7 | exon | 17493593 | 17494006 | 17493593 | ID=MsG0780036982.01.T01:exon:3284;Parent=MsG0780036982.01.T01 |
| Chr7 | CDS | 17493593 | 17494006 | 17493593 | ID=MsG0780036982.01.T01:cds;Parent=MsG0780036982.01.T01 |
| Gene Sequence |
| Protein sequence |
