Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036983.01.T01 | XP_013447886.1 | 95.62 | 137 | 6 | 0 | 1 | 137 | 1 | 137 | 1.04E-89 | 266 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036983.01.T01 | A0A072TYJ6 | 95.620 | 137 | 6 | 0 | 1 | 137 | 1 | 137 | 4.96e-90 | 266 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0780036981.01 | MsG0780036983.01 | 0.804570 | 2.027745e-49 | 1.049058e-46 |
| MsG0780036982.01 | MsG0780036983.01 | 0.873951 | 9.874676e-68 | 4.364724e-64 |
| MsG0780036983.01 | MsG0780036984.01 | 0.843978 | 1.004722e-58 | 1.598867e-55 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036983.01.T01 | MTR_7g024873 | 95.620 | 137 | 6 | 0 | 1 | 137 | 1 | 137 | 1.26e-93 | 266 |
| MsG0780036983.01.T01 | MTR_7g024890 | 91.971 | 137 | 11 | 0 | 1 | 137 | 1 | 137 | 4.57e-90 | 258 |
| MsG0780036983.01.T01 | MTR_7g024880 | 90.511 | 137 | 13 | 0 | 1 | 137 | 1 | 137 | 3.16e-88 | 253 |
| MsG0780036983.01.T01 | MTR_8g446810 | 50.714 | 140 | 66 | 1 | 1 | 137 | 1 | 140 | 4.97e-43 | 139 |
| MsG0780036983.01.T01 | MTR_7g024900 | 90.196 | 51 | 5 | 0 | 1 | 51 | 1 | 51 | 7.34e-27 | 95.1 |
| MsG0780036983.01.T01 | MTR_1g081270 | 36.641 | 131 | 78 | 1 | 1 | 131 | 1 | 126 | 2.37e-20 | 80.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036983.01.T01 | AT2G44310 | 47.445 | 137 | 70 | 1 | 1 | 135 | 1 | 137 | 9.40e-41 | 133 |
Find 27 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGATGAGTGTAGAAATTT+TGG | 0.159822 | 7:-17508417 | None:intergenic |
| TAAACACGAGCAAGCTCATT+TGG | 0.321898 | 7:+17508215 | None:intergenic |
| CATTTAAGCAGCCACTTTAC+CGG | 0.324149 | 7:+17508019 | None:intergenic |
| TAAATGCTTCTTCATCTTCA+AGG | 0.379890 | 7:+17508372 | None:intergenic |
| TCTTCCAAGACCATTTGAAT+AGG | 0.402040 | 7:+17508077 | None:intergenic |
| CATGCCTATTCAAATGGTCT+TGG | 0.421269 | 7:-17508081 | MsG0780036983.01.T01:CDS |
| GGGTTTCATGCCTATTCAAA+TGG | 0.441768 | 7:-17508087 | MsG0780036983.01.T01:CDS |
| CTTCTTCATCTTCAAGGAAG+TGG | 0.447415 | 7:+17508378 | None:intergenic |
| CTCCTTCAACATCTCATCAT+AGG | 0.454389 | 7:+17508292 | None:intergenic |
| AAACACGAGCAAGCTCATTT+GGG | 0.456333 | 7:+17508216 | None:intergenic |
| TTAATAGCTCGATAGGAAAT+CGG | 0.457868 | 7:-17508352 | MsG0780036983.01.T01:CDS |
| CTCCTATGATGAGATGTTGA+AGG | 0.467641 | 7:-17508294 | MsG0780036983.01.T01:CDS |
| CAGTTCGATCACAACTTGAA+TGG | 0.485615 | 7:-17508176 | MsG0780036983.01.T01:CDS |
| GAAGCATTTAATAGCTCGAT+AGG | 0.503824 | 7:-17508359 | MsG0780036983.01.T01:CDS |
| GCTTGCAATGGCAGATGGAA+TGG | 0.505770 | 7:-17508108 | MsG0780036983.01.T01:CDS |
| AGGGTCTTTGAAACACACTT+TGG | 0.514624 | 7:-17508257 | MsG0780036983.01.T01:CDS |
| CTTGCAATGGCAGATGGAAT+GGG | 0.523875 | 7:-17508107 | MsG0780036983.01.T01:CDS |
| ATAGCTGTTGAAAGAGAGTC+CGG | 0.527137 | 7:-17508038 | MsG0780036983.01.T01:CDS |
| GAAGGAGCTCAAGAGCTTGA+GGG | 0.528370 | 7:-17508276 | MsG0780036983.01.T01:CDS |
| ATCATGCTTGCAATGGCAGA+TGG | 0.538343 | 7:-17508113 | MsG0780036983.01.T01:CDS |
| TGAAAGAGAGTCCGGTAAAG+TGG | 0.560086 | 7:-17508030 | MsG0780036983.01.T01:CDS |
| TGAAGGAGCTCAAGAGCTTG+AGG | 0.569381 | 7:-17508277 | MsG0780036983.01.T01:CDS |
| GACCGTCGTTGTTTGTGTCG+AGG | 0.576811 | 7:+17508321 | None:intergenic |
| AACACACTTTGGTATTGATG+TGG | 0.581840 | 7:-17508246 | MsG0780036983.01.T01:CDS |
| TTCAAGGAAGTGGACAATTG+TGG | 0.596143 | 7:+17508388 | None:intergenic |
| TAAGCAAATCATGCTTGCAA+TGG | 0.600597 | 7:-17508120 | MsG0780036983.01.T01:CDS |
| TGCCTCGACACAAACAACGA+CGG | 0.702196 | 7:-17508323 | MsG0780036983.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TAAATGCTTCTTCATCTTCA+AGG | + | Chr7:17508066-17508085 | None:intergenic | 30.0% | |
| TTAATAGCTCGATAGGAAAT+CGG | - | Chr7:17508083-17508102 | MsG0780036983.01.T01:CDS | 30.0% | |
| ! | ATGAGTGTAGAAATTTTGGA+TGG | - | Chr7:17508022-17508041 | MsG0780036983.01.T01:CDS | 30.0% |
| !! | CTCTTTCAACAGCTATTTTA+AGG | + | Chr7:17508393-17508412 | None:intergenic | 30.0% |
| TAAGCAAATCATGCTTGCAA+TGG | - | Chr7:17508315-17508334 | MsG0780036983.01.T01:CDS | 35.0% | |
| TCTTCCAAGACCATTTGAAT+AGG | + | Chr7:17508361-17508380 | None:intergenic | 35.0% | |
| ! | GAAGCATTTAATAGCTCGAT+AGG | - | Chr7:17508076-17508095 | MsG0780036983.01.T01:CDS | 35.0% |
| !! | AACACACTTTGGTATTGATG+TGG | - | Chr7:17508189-17508208 | MsG0780036983.01.T01:CDS | 35.0% |
| AAACACGAGCAAGCTCATTT+GGG | + | Chr7:17508222-17508241 | None:intergenic | 40.0% | |
| ATAGCTGTTGAAAGAGAGTC+CGG | - | Chr7:17508397-17508416 | MsG0780036983.01.T01:CDS | 40.0% | |
| CAGTTCGATCACAACTTGAA+TGG | - | Chr7:17508259-17508278 | MsG0780036983.01.T01:CDS | 40.0% | |
| CATGCCTATTCAAATGGTCT+TGG | - | Chr7:17508354-17508373 | MsG0780036983.01.T01:CDS | 40.0% | |
| CTCCTATGATGAGATGTTGA+AGG | - | Chr7:17508141-17508160 | MsG0780036983.01.T01:CDS | 40.0% | |
| CTCCTTCAACATCTCATCAT+AGG | + | Chr7:17508146-17508165 | None:intergenic | 40.0% | |
| CTTCTTCATCTTCAAGGAAG+TGG | + | Chr7:17508060-17508079 | None:intergenic | 40.0% | |
| GGGTTTCATGCCTATTCAAA+TGG | - | Chr7:17508348-17508367 | MsG0780036983.01.T01:CDS | 40.0% | |
| TAAACACGAGCAAGCTCATT+TGG | + | Chr7:17508223-17508242 | None:intergenic | 40.0% | |
| TTCAAGGAAGTGGACAATTG+TGG | + | Chr7:17508050-17508069 | None:intergenic | 40.0% | |
| !! | AGGGTCTTTGAAACACACTT+TGG | - | Chr7:17508178-17508197 | MsG0780036983.01.T01:CDS | 40.0% |
| ATCATGCTTGCAATGGCAGA+TGG | - | Chr7:17508322-17508341 | MsG0780036983.01.T01:CDS | 45.0% | |
| CTTGCAATGGCAGATGGAAT+GGG | - | Chr7:17508328-17508347 | MsG0780036983.01.T01:CDS | 45.0% | |
| TGAAAGAGAGTCCGGTAAAG+TGG | - | Chr7:17508405-17508424 | MsG0780036983.01.T01:CDS | 45.0% | |
| GAAGGAGCTCAAGAGCTTGA+GGG | - | Chr7:17508159-17508178 | MsG0780036983.01.T01:CDS | 50.0% | |
| GCTTGCAATGGCAGATGGAA+TGG | - | Chr7:17508327-17508346 | MsG0780036983.01.T01:CDS | 50.0% | |
| TGAAGGAGCTCAAGAGCTTG+AGG | - | Chr7:17508158-17508177 | MsG0780036983.01.T01:CDS | 50.0% | |
| TGCCTCGACACAAACAACGA+CGG | - | Chr7:17508112-17508131 | MsG0780036983.01.T01:CDS | 50.0% | |
| GACCGTCGTTGTTTGTGTCG+AGG | + | Chr7:17508117-17508136 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 17508022 | 17508435 | 17508022 | ID=MsG0780036983.01;Name=MsG0780036983.01 |
| Chr7 | mRNA | 17508022 | 17508435 | 17508022 | ID=MsG0780036983.01.T01;Parent=MsG0780036983.01;Name=MsG0780036983.01.T01;_AED=0.31;_eAED=0.31;_QI=0|-1|0|1|-1|1|1|0|137 |
| Chr7 | exon | 17508022 | 17508435 | 17508022 | ID=MsG0780036983.01.T01:exon:3285;Parent=MsG0780036983.01.T01 |
| Chr7 | CDS | 17508022 | 17508435 | 17508022 | ID=MsG0780036983.01.T01:cds;Parent=MsG0780036983.01.T01 |
| Gene Sequence |
| Protein sequence |
