Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036984.01.T01 | XP_013447886.1 | 94.891 | 137 | 7 | 0 | 1 | 137 | 1 | 137 | 1.58E-89 | 266 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036984.01.T01 | A0A072TYJ6 | 94.891 | 137 | 7 | 0 | 1 | 137 | 1 | 137 | 7.53e-90 | 266 |
| MsG0780036984.01.T01 | A0A396GWC2 | 94.891 | 137 | 7 | 0 | 1 | 137 | 18 | 154 | 1.60e-89 | 266 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048889.01 | MsG0780036984.01 | 0.841281 | 5.225093e-58 | 7.639468e-55 |
| MsG0080049008.01 | MsG0780036984.01 | 0.829999 | 3.758648e-55 | 3.910150e-52 |
| MsG0180000466.01 | MsG0780036984.01 | 0.829035 | 6.447716e-55 | 6.520714e-52 |
| MsG0180000551.01 | MsG0780036984.01 | 0.817049 | 4.033652e-52 | 2.906505e-49 |
| MsG0180000552.01 | MsG0780036984.01 | 0.816364 | 5.744468e-52 | 4.062308e-49 |
| MsG0180001029.01 | MsG0780036984.01 | 0.805422 | 1.345404e-49 | 7.114897e-47 |
| MsG0180003250.01 | MsG0780036984.01 | 0.802571 | 5.267987e-49 | 2.589167e-46 |
| MsG0180003405.01 | MsG0780036984.01 | -0.811574 | 6.539914e-51 | 4.062813e-48 |
| MsG0180003484.01 | MsG0780036984.01 | 0.824909 | 6.250094e-54 | 5.612112e-51 |
| MsG0180004737.01 | MsG0780036984.01 | -0.807905 | 4.021532e-50 | 2.267232e-47 |
| MsG0180004787.01 | MsG0780036984.01 | -0.828603 | 8.200631e-55 | 8.190649e-52 |
| MsG0180004830.01 | MsG0780036984.01 | 0.803358 | 3.622291e-49 | 1.816447e-46 |
| MsG0180005984.01 | MsG0780036984.01 | 0.818248 | 2.164060e-52 | 1.611342e-49 |
| MsG0280010936.01 | MsG0780036984.01 | 0.815868 | 7.416480e-52 | 5.173675e-49 |
| MsG0280011236.01 | MsG0780036984.01 | 0.803437 | 3.488288e-49 | 1.752882e-46 |
| MsG0280011269.01 | MsG0780036984.01 | 0.807130 | 5.873110e-50 | 3.245030e-47 |
| MsG0480022428.01 | MsG0780036984.01 | 0.833037 | 6.712597e-56 | 7.638551e-53 |
| MsG0480023067.01 | MsG0780036984.01 | 0.818818 | 1.607312e-52 | 1.215779e-49 |
| MsG0480023242.01 | MsG0780036984.01 | -0.806928 | 6.481845e-50 | 3.562752e-47 |
| MsG0480023244.01 | MsG0780036984.01 | -0.831208 | 1.901982e-55 | 2.049803e-52 |
| MsG0480023851.01 | MsG0780036984.01 | -0.813321 | 2.714977e-51 | 1.767395e-48 |
| MsG0580024087.01 | MsG0780036984.01 | 0.802764 | 4.804619e-49 | 2.373295e-46 |
| MsG0580025153.01 | MsG0780036984.01 | -0.805831 | 1.103810e-49 | 5.899182e-47 |
| MsG0580025633.01 | MsG0780036984.01 | 0.807136 | 5.856205e-50 | 3.236229e-47 |
| MsG0380015711.01 | MsG0780036984.01 | 0.806205 | 9.211327e-50 | 4.970278e-47 |
| MsG0380016300.01 | MsG0780036984.01 | 0.815072 | 1.114826e-51 | 7.609267e-49 |
| MsG0380017030.01 | MsG0780036984.01 | 0.800861 | 1.181456e-48 | 5.560754e-46 |
| MsG0380017901.01 | MsG0780036984.01 | 0.812863 | 3.422441e-51 | 2.200370e-48 |
| MsG0580027638.01 | MsG0780036984.01 | 0.806033 | 1.001003e-49 | 5.377443e-47 |
| MsG0580028490.01 | MsG0780036984.01 | 0.805607 | 1.230437e-49 | 6.537844e-47 |
| MsG0580029296.01 | MsG0780036984.01 | 0.819593 | 1.070359e-52 | 8.272581e-50 |
| MsG0580029696.01 | MsG0780036984.01 | 0.826229 | 3.041453e-54 | 2.835488e-51 |
| MsG0680030296.01 | MsG0780036984.01 | 0.869627 | 2.686812e-66 | 1.015149e-62 |
| MsG0680030613.01 | MsG0780036984.01 | 0.801906 | 7.217136e-49 | 3.487786e-46 |
| MsG0780036981.01 | MsG0780036984.01 | 0.831081 | 2.043343e-55 | 2.194064e-52 |
| MsG0780036982.01 | MsG0780036984.01 | 0.863588 | 2.235826e-64 | 6.813317e-61 |
| MsG0780036983.01 | MsG0780036984.01 | 0.843978 | 1.004722e-58 | 1.598867e-55 |
| MsG0780036984.01 | MsG0780038305.01 | 0.841166 | 5.603082e-58 | 8.163131e-55 |
| MsG0780036984.01 | MsG0780038307.01 | 0.810103 | 1.361203e-50 | 8.133677e-48 |
| MsG0780036984.01 | MsG0780038914.01 | 0.819981 | 8.730573e-53 | 6.821287e-50 |
| MsG0780036984.01 | MsG0780038919.01 | 0.804969 | 1.673292e-49 | 8.746849e-47 |
| MsG0780036984.01 | MsG0780039234.01 | 0.805272 | 1.446060e-49 | 7.617707e-47 |
| MsG0780036984.01 | MsG0780039431.01 | 0.807202 | 5.672920e-50 | 3.140393e-47 |
| MsG0780036984.01 | MsG0780039979.01 | 0.817390 | 3.379826e-52 | 2.458263e-49 |
| MsG0780036984.01 | MsG0780041180.01 | 0.809521 | 1.816174e-50 | 1.068679e-47 |
| MsG0780036984.01 | MsG0780041436.01 | 0.805596 | 1.236934e-49 | 6.570518e-47 |
| MsG0780036984.01 | MsG0880042122.01 | 0.811147 | 8.097732e-51 | 4.974146e-48 |
| MsG0780036984.01 | MsG0880043212.01 | 0.807579 | 4.718456e-50 | 2.637579e-47 |
| MsG0780036984.01 | MsG0880043669.01 | 0.818831 | 1.596211e-52 | 1.207889e-49 |
| MsG0780036984.01 | MsG0880043906.01 | 0.809588 | 1.756506e-50 | 1.035482e-47 |
| MsG0780036984.01 | MsG0880045761.01 | -0.801052 | 1.079951e-48 | 5.107793e-46 |
| MsG0780036984.01 | MsG0880046010.01 | -0.803857 | 2.853836e-49 | 1.449683e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036984.01.T01 | MTR_7g024873 | 94.891 | 137 | 7 | 0 | 1 | 137 | 1 | 137 | 1.91e-93 | 266 |
| MsG0780036984.01.T01 | MTR_7g024880 | 94.161 | 137 | 8 | 0 | 1 | 137 | 1 | 137 | 1.03e-91 | 261 |
| MsG0780036984.01.T01 | MTR_7g024890 | 94.161 | 137 | 8 | 0 | 1 | 137 | 1 | 137 | 1.51e-91 | 261 |
| MsG0780036984.01.T01 | MTR_8g446810 | 48.571 | 140 | 69 | 1 | 1 | 137 | 1 | 140 | 1.39e-41 | 135 |
| MsG0780036984.01.T01 | MTR_7g024900 | 94.118 | 51 | 3 | 0 | 1 | 51 | 1 | 51 | 3.49e-28 | 98.6 |
| MsG0780036984.01.T01 | MTR_1g081270 | 35.878 | 131 | 79 | 1 | 1 | 131 | 1 | 126 | 2.57e-19 | 78.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036984.01.T01 | AT2G44310 | 46.715 | 137 | 71 | 1 | 1 | 135 | 1 | 137 | 7.24e-40 | 130 |
Find 26 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGATGAGTGTAGAAATTT+TGG | 0.159822 | 7:-17513281 | None:intergenic |
| CTTGAATGGCACAATTGATT+TGG | 0.268459 | 7:-17513026 | MsG0780036984.01.T01:CDS |
| TAAACACGAGCAAGCTCGTT+TGG | 0.337682 | 7:+17513079 | None:intergenic |
| TAAATGCTTCTTCATCTTCA+AGG | 0.379890 | 7:+17513236 | None:intergenic |
| CATGCCAATTCAAATGGTCT+TGG | 0.391639 | 7:-17512945 | MsG0780036984.01.T01:CDS |
| TCTTCCAAGACCATTTGAAT+TGG | 0.395041 | 7:+17512941 | None:intergenic |
| TTAATAACTCAGTTGGAAAT+CGG | 0.405538 | 7:-17513216 | MsG0780036984.01.T01:CDS |
| CTTCTTCATCTTCAAGGAAG+TGG | 0.447415 | 7:+17513242 | None:intergenic |
| GGGTTTCATGCCAATTCAAA+TGG | 0.458206 | 7:-17512951 | MsG0780036984.01.T01:CDS |
| GAAGCATTTAATAACTCAGT+TGG | 0.458411 | 7:-17513223 | MsG0780036984.01.T01:CDS |
| AAACACGAGCAAGCTCGTTT+GGG | 0.462526 | 7:+17513080 | None:intergenic |
| GAAGGAGCTCCGGAGCTTGA+GGG | 0.481338 | 7:-17513140 | MsG0780036984.01.T01:CDS |
| CAGTTCGATCACAACTTGAA+TGG | 0.485615 | 7:-17513040 | MsG0780036984.01.T01:CDS |
| CTCTTATGATGAGATGTTGA+AGG | 0.511648 | 7:-17513158 | MsG0780036984.01.T01:CDS |
| GCTTGCAATGGCAGATGGGA+TGG | 0.521128 | 7:-17512972 | MsG0780036984.01.T01:CDS |
| TGAAGGAGCTCCGGAGCTTG+AGG | 0.522904 | 7:-17513141 | MsG0780036984.01.T01:CDS |
| ATGAGATGTTGAAGGAGCTC+CGG | 0.525969 | 7:-17513150 | MsG0780036984.01.T01:CDS |
| TGAAAGAGAGTACAATAAAG+TGG | 0.551009 | 7:-17512894 | MsG0780036984.01.T01:CDS |
| CTTGCAATGGCAGATGGGAT+GGG | 0.557101 | 7:-17512971 | MsG0780036984.01.T01:CDS |
| ATGATGCTTGCAATGGCAGA+TGG | 0.558215 | 7:-17512977 | MsG0780036984.01.T01:CDS |
| AACACACTTTGGTATTGATG+TGG | 0.581840 | 7:-17513110 | MsG0780036984.01.T01:CDS |
| TTCAAGGAAGTGGACAATTG+TGG | 0.596143 | 7:+17513252 | None:intergenic |
| CGCCTCGACACAAACAGCGA+TGG | 0.598011 | 7:-17513187 | MsG0780036984.01.T01:CDS |
| AAAGCAGATGATGCTTGCAA+TGG | 0.609930 | 7:-17512984 | MsG0780036984.01.T01:CDS |
| GACCATCGCTGTTTGTGTCG+AGG | 0.621315 | 7:+17513185 | None:intergenic |
| TGATGCTTGCAATGGCAGAT+GGG | 0.625156 | 7:-17512976 | MsG0780036984.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTAATAACTCAGTTGGAAAT+CGG | - | Chr7:17512947-17512966 | MsG0780036984.01.T01:CDS | 25.0% |
| TAAATGCTTCTTCATCTTCA+AGG | + | Chr7:17512930-17512949 | None:intergenic | 30.0% | |
| ! | ATGAGTGTAGAAATTTTGGA+TGG | - | Chr7:17512886-17512905 | MsG0780036984.01.T01:CDS | 30.0% |
| ! | GAAGCATTTAATAACTCAGT+TGG | - | Chr7:17512940-17512959 | MsG0780036984.01.T01:CDS | 30.0% |
| ! | TGAAAGAGAGTACAATAAAG+TGG | - | Chr7:17513269-17513288 | MsG0780036984.01.T01:CDS | 30.0% |
| CTCTTATGATGAGATGTTGA+AGG | - | Chr7:17513005-17513024 | MsG0780036984.01.T01:CDS | 35.0% | |
| CTTGAATGGCACAATTGATT+TGG | - | Chr7:17513137-17513156 | MsG0780036984.01.T01:CDS | 35.0% | |
| TCTTCCAAGACCATTTGAAT+TGG | + | Chr7:17513225-17513244 | None:intergenic | 35.0% | |
| !! | AACACACTTTGGTATTGATG+TGG | - | Chr7:17513053-17513072 | MsG0780036984.01.T01:CDS | 35.0% |
| !! | CTCTTTCAACAGCTATTTTG+AGG | + | Chr7:17513257-17513276 | None:intergenic | 35.0% |
| !!! | AGGGTTTTTGAAACACACTT+TGG | - | Chr7:17513042-17513061 | MsG0780036984.01.T01:CDS | 35.0% |
| AAAGCAGATGATGCTTGCAA+TGG | - | Chr7:17513179-17513198 | MsG0780036984.01.T01:CDS | 40.0% | |
| CAGTTCGATCACAACTTGAA+TGG | - | Chr7:17513123-17513142 | MsG0780036984.01.T01:CDS | 40.0% | |
| CATGCCAATTCAAATGGTCT+TGG | - | Chr7:17513218-17513237 | MsG0780036984.01.T01:CDS | 40.0% | |
| CTTCTTCATCTTCAAGGAAG+TGG | + | Chr7:17512924-17512943 | None:intergenic | 40.0% | |
| GGGTTTCATGCCAATTCAAA+TGG | - | Chr7:17513212-17513231 | MsG0780036984.01.T01:CDS | 40.0% | |
| TTCAAGGAAGTGGACAATTG+TGG | + | Chr7:17512914-17512933 | None:intergenic | 40.0% | |
| TTTCAAAAACCCTCAAGCTC+CGG | + | Chr7:17513035-17513054 | None:intergenic | 40.0% | |
| AAACACGAGCAAGCTCGTTT+GGG | + | Chr7:17513086-17513105 | None:intergenic | 45.0% | |
| ATGAGATGTTGAAGGAGCTC+CGG | - | Chr7:17513013-17513032 | MsG0780036984.01.T01:CDS | 45.0% | |
| ATGATGCTTGCAATGGCAGA+TGG | - | Chr7:17513186-17513205 | MsG0780036984.01.T01:CDS | 45.0% | |
| TAAACACGAGCAAGCTCGTT+TGG | + | Chr7:17513087-17513106 | None:intergenic | 45.0% | |
| TGATGCTTGCAATGGCAGAT+GGG | - | Chr7:17513187-17513206 | MsG0780036984.01.T01:CDS | 45.0% | |
| CTTGCAATGGCAGATGGGAT+GGG | - | Chr7:17513192-17513211 | MsG0780036984.01.T01:CDS | 50.0% | |
| GACCATCGCTGTTTGTGTCG+AGG | + | Chr7:17512981-17513000 | None:intergenic | 55.0% | |
| GCTTGCAATGGCAGATGGGA+TGG | - | Chr7:17513191-17513210 | MsG0780036984.01.T01:CDS | 55.0% | |
| CGCCTCGACACAAACAGCGA+TGG | - | Chr7:17512976-17512995 | MsG0780036984.01.T01:CDS | 60.0% | |
| GAAGGAGCTCCGGAGCTTGA+GGG | - | Chr7:17513023-17513042 | MsG0780036984.01.T01:CDS | 60.0% | |
| TGAAGGAGCTCCGGAGCTTG+AGG | - | Chr7:17513022-17513041 | MsG0780036984.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 17512886 | 17513299 | 17512886 | ID=MsG0780036984.01;Name=MsG0780036984.01 |
| Chr7 | mRNA | 17512886 | 17513299 | 17512886 | ID=MsG0780036984.01.T01;Parent=MsG0780036984.01;Name=MsG0780036984.01.T01;_AED=0.31;_eAED=0.31;_QI=0|-1|0|1|-1|1|1|0|137 |
| Chr7 | exon | 17512886 | 17513299 | 17512886 | ID=MsG0780036984.01.T01:exon:3286;Parent=MsG0780036984.01.T01 |
| Chr7 | CDS | 17512886 | 17513299 | 17512886 | ID=MsG0780036984.01.T01:cds;Parent=MsG0780036984.01.T01 |
| Gene Sequence |
| Protein sequence |
