Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039386.01.T01 | XP_003623570.1 | 60.586 | 307 | 30 | 5 | 2 | 219 | 118 | 422 | 1.77E-92 | 292 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039386.01.T01 | Q5XVC7 | 35.16 | 219 | 89 | 7 | 52 | 219 | 166 | 382 | 1.29E-15 | 78.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039386.01.T01 | G7KX28 | 60.586 | 307 | 30 | 5 | 2 | 219 | 118 | 422 | 8.46e-93 | 292 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000155.01 | MsG0780039386.01 | 0.800618 | 1.324572e-48 | 6.195771e-46 |
| MsG0180005575.01 | MsG0780039386.01 | 0.812105 | 5.011681e-51 | 3.157971e-48 |
| MsG0680034853.01 | MsG0780039386.01 | 0.821233 | 4.500954e-53 | 3.641050e-50 |
| MsG0380016970.01 | MsG0780039386.01 | 0.800212 | 1.602389e-48 | 7.418435e-46 |
| MsG0580027925.01 | MsG0780039386.01 | 0.801638 | 8.191310e-49 | 3.931621e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039386.01.T01 | MTR_7g072550 | 60.586 | 307 | 30 | 5 | 2 | 219 | 118 | 422 | 2.15e-96 | 292 |
| MsG0780039386.01.T01 | MTR_4g029090 | 41.667 | 264 | 58 | 7 | 48 | 219 | 152 | 411 | 1.81e-35 | 131 |
| MsG0780039386.01.T01 | MTR_5g083980 | 42.857 | 70 | 40 | 0 | 45 | 114 | 149 | 218 | 4.62e-13 | 67.8 |
| MsG0780039386.01.T01 | MTR_3g072350 | 40.426 | 94 | 46 | 2 | 46 | 139 | 158 | 241 | 9.74e-13 | 67.0 |
| MsG0780039386.01.T01 | MTR_3g072350 | 40.426 | 94 | 46 | 2 | 46 | 139 | 158 | 241 | 1.06e-12 | 67.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039386.01.T01 | AT2G40480 | 35.160 | 219 | 89 | 7 | 52 | 219 | 166 | 382 | 1.32e-16 | 78.2 |
| MsG0780039386.01.T01 | AT2G38370 | 31.474 | 251 | 85 | 4 | 50 | 219 | 141 | 385 | 6.45e-16 | 76.3 |
| MsG0780039386.01.T01 | AT2G38370 | 31.225 | 253 | 87 | 4 | 48 | 219 | 122 | 368 | 6.73e-16 | 76.3 |
| MsG0780039386.01.T01 | AT3G51720 | 35.429 | 175 | 87 | 3 | 58 | 208 | 104 | 276 | 8.97e-16 | 75.5 |
Find 47 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTTCAAGGGCCTTCTTGC+AGG | 0.231352 | 7:-63250856 | None:intergenic |
| GGCAATATCATAAGTAGTTT+TGG | 0.285269 | 7:-63250226 | None:intergenic |
| AACCAAAGCCCACCTGATTT+CGG | 0.326241 | 7:-63250556 | None:intergenic |
| TGCCGAAATCAGGTGGGCTT+TGG | 0.372524 | 7:+63250554 | MsG0780039386.01.T01:CDS |
| TTCTCCAGACTTGATACTAA+TGG | 0.384913 | 7:+63250176 | MsG0780039386.01.T01:CDS |
| CCCTTTGTAAAGCTTCTTCA+AGG | 0.390431 | 7:-63250869 | None:intergenic |
| TTAGAAGTTGAAGAGGCTTA+AGG | 0.392610 | 7:+63250915 | MsG0780039386.01.T01:CDS |
| ACTCTTCTGGTGTACCTTCA+TGG | 0.393517 | 7:-63250650 | None:intergenic |
| TGATAAGAACTGCCGAAATC+AGG | 0.420953 | 7:+63250544 | MsG0780039386.01.T01:CDS |
| AATCAGGTGGGCTTTGGTTG+CGG | 0.450994 | 7:+63250560 | MsG0780039386.01.T01:CDS |
| CTGAAACGATATTGAATCTC+GGG | 0.455182 | 7:+63250037 | MsG0780039386.01.T01:CDS |
| AGACCATGCGTTATTTCTGC+AGG | 0.456175 | 7:-63250625 | None:intergenic |
| ATCGAATGTAGATGAGAAAG+AGG | 0.464370 | 7:+63250080 | MsG0780039386.01.T01:CDS |
| AGGCATAGAACATTCCTTCA+TGG | 0.469854 | 7:-63250142 | None:intergenic |
| ATAAGAAATTAGAGAAAGAA+AGG | 0.483070 | 7:+63250277 | MsG0780039386.01.T01:CDS |
| TGTAGATGAGAAAGAGGTAA+AGG | 0.485166 | 7:+63250086 | MsG0780039386.01.T01:CDS |
| AGGGTAGAGGCTGCAGATAG+AGG | 0.502926 | 7:+63250888 | MsG0780039386.01.T01:CDS |
| AGGGAAGTTAGAAGTTGAAG+AGG | 0.506157 | 7:+63250908 | MsG0780039386.01.T01:CDS |
| GGGTAGAGGCTGCAGATAGA+GGG | 0.517190 | 7:+63250889 | MsG0780039386.01.T01:CDS |
| AATTCCATTAGTATCAAGTC+TGG | 0.517670 | 7:-63250180 | None:intergenic |
| GGCATAGAACATTCCTTCAT+GGG | 0.522442 | 7:-63250141 | None:intergenic |
| TCTGAAACGATATTGAATCT+CGG | 0.530021 | 7:+63250036 | MsG0780039386.01.T01:CDS |
| GGTTGCGGCAAGGAAAATGA+AGG | 0.540963 | 7:+63250575 | MsG0780039386.01.T01:CDS |
| GATACTAATGGAATTGCAGC+AGG | 0.554166 | 7:+63250188 | MsG0780039386.01.T01:CDS |
| GAACCTCAGACGGACTCGAT+TGG | 0.556300 | 7:-63250113 | None:intergenic |
| CCTTGAAGAAGCTTTACAAA+GGG | 0.558535 | 7:+63250869 | MsG0780039386.01.T01:CDS |
| TTTGAAATCAATGCTGCAGA+AGG | 0.560044 | 7:+63250008 | None:intergenic |
| CTCGCCTTTGAACTCATCGA+TGG | 0.561820 | 7:-63250444 | None:intergenic |
| AACGCATGGTCTAGCCATGA+AGG | 0.562071 | 7:+63250636 | MsG0780039386.01.T01:CDS |
| TGAGGTTCTTCAACCCATGA+AGG | 0.563383 | 7:+63250128 | MsG0780039386.01.T01:CDS |
| AGAAGCTTTACAAAGGGTAG+AGG | 0.563612 | 7:+63250875 | MsG0780039386.01.T01:CDS |
| TTCAACAGTAGCTCGTACAT+CGG | 0.575007 | 7:-63250247 | None:intergenic |
| CCTTTGTAAAGCTTCTTCAA+GGG | 0.580544 | 7:-63250868 | None:intergenic |
| AGAAGTTGAAACCTGCAAGA+AGG | 0.592428 | 7:+63250845 | MsG0780039386.01.T01:CDS |
| ATGGGTTGAAGAACCTCAGA+CGG | 0.594605 | 7:-63250123 | None:intergenic |
| TAAGAACTGCCGAAATCAGG+TGG | 0.597549 | 7:+63250547 | MsG0780039386.01.T01:CDS |
| GGTGGGCTTTGGTTGCGGCA+AGG | 0.602291 | 7:+63250565 | MsG0780039386.01.T01:CDS |
| CCCTTGAAGAAGCTTTACAA+AGG | 0.607687 | 7:+63250868 | MsG0780039386.01.T01:CDS |
| AACTCCATCGATGAGTTCAA+AGG | 0.609366 | 7:+63250440 | MsG0780039386.01.T01:CDS |
| GACCATGCGTTATTTCTGCA+GGG | 0.621674 | 7:-63250624 | None:intergenic |
| AAACCAATCGAGTCCGTCTG+AGG | 0.623782 | 7:+63250110 | MsG0780039386.01.T01:CDS |
| TACCCTGCAGAAATAACGCA+TGG | 0.637574 | 7:+63250622 | MsG0780039386.01.T01:CDS |
| TCAAGTCTGGAGAAGATACA+CGG | 0.640414 | 7:-63250167 | None:intergenic |
| CAAGTCTGGAGAAGATACAC+GGG | 0.678628 | 7:-63250166 | None:intergenic |
| TCTGGAGAAGATACACGGGA+AGG | 0.691888 | 7:-63250162 | None:intergenic |
| AAGAACTGCCGAAATCAGGT+GGG | 0.692468 | 7:+63250548 | MsG0780039386.01.T01:CDS |
| CACTTGAGAAAACCCGTGAG+AGG | 0.700246 | 7:+63250304 | MsG0780039386.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAGAAATTAGAGAAAGAA+AGG | + | Chr7:63250277-63250296 | MsG0780039386.01.T01:CDS | 20.0% |
| !!! | TTTTAAAACTTCTGATTTTG+CGG | - | Chr7:63250493-63250512 | None:intergenic | 20.0% |
| !!! | TATCATAAGTAGTTTTGGTT+AGG | - | Chr7:63250224-63250243 | None:intergenic | 25.0% |
| AATTCCATTAGTATCAAGTC+TGG | - | Chr7:63250183-63250202 | None:intergenic | 30.0% | |
| AGTTCTGTTGTAACATCTAA+AGG | - | Chr7:63250424-63250443 | None:intergenic | 30.0% | |
| ! | GGCAATATCATAAGTAGTTT+TGG | - | Chr7:63250229-63250248 | None:intergenic | 30.0% |
| ! | TCTGAAACGATATTGAATCT+CGG | + | Chr7:63250036-63250055 | MsG0780039386.01.T01:CDS | 30.0% |
| !! | CGCAGATGGATATTTTTAAA+GGG | + | Chr7:63250806-63250825 | MsG0780039386.01.T01:intron | 30.0% |
| !! | TCGCAGATGGATATTTTTAA+AGG | + | Chr7:63250805-63250824 | MsG0780039386.01.T01:intron | 30.0% |
| !! | TGGATATTTTTAAAGGGTAG+AGG | + | Chr7:63250812-63250831 | MsG0780039386.01.T01:intron | 30.0% |
| ATCGAATGTAGATGAGAAAG+AGG | + | Chr7:63250080-63250099 | MsG0780039386.01.T01:CDS | 35.0% | |
| CCTTGAAGAAGCTTTACAAA+GGG | + | Chr7:63250869-63250888 | MsG0780039386.01.T01:CDS | 35.0% | |
| CCTTTGTAAAGCTTCTTCAA+GGG | - | Chr7:63250871-63250890 | None:intergenic | 35.0% | |
| CTGAAACGATATTGAATCTC+GGG | + | Chr7:63250037-63250056 | MsG0780039386.01.T01:CDS | 35.0% | |
| TGTAGATGAGAAAGAGGTAA+AGG | + | Chr7:63250086-63250105 | MsG0780039386.01.T01:CDS | 35.0% | |
| TTCTCCAGACTTGATACTAA+TGG | + | Chr7:63250176-63250195 | MsG0780039386.01.T01:CDS | 35.0% | |
| !! | AATTTTGAGTAACCTCTCAC+GGG | - | Chr7:63250319-63250338 | None:intergenic | 35.0% |
| !! | CAATTTTGAGTAACCTCTCA+CGG | - | Chr7:63250320-63250339 | None:intergenic | 35.0% |
| !!! | AGTTTTGGTTAGGTTCAACT+TGG | - | Chr7:63250214-63250233 | None:intergenic | 35.0% |
| AACCAGATTAAGACTGCAAG+TGG | + | Chr7:63250375-63250394 | MsG0780039386.01.T01:intron | 40.0% | |
| AACTCCATCGATGAGTTCAA+AGG | + | Chr7:63250440-63250459 | MsG0780039386.01.T01:CDS | 40.0% | |
| AGAAGCTTTACAAAGGGTAG+AGG | + | Chr7:63250875-63250894 | MsG0780039386.01.T01:CDS | 40.0% | |
| AGAAGTTGAAACCTGCAAGA+AGG | + | Chr7:63250845-63250864 | MsG0780039386.01.T01:CDS | 40.0% | |
| AGGCATAGAACATTCCTTCA+TGG | - | Chr7:63250145-63250164 | None:intergenic | 40.0% | |
| AGGGAAGTTAGAAGTTGAAG+AGG | + | Chr7:63250908-63250927 | MsG0780039386.01.T01:CDS | 40.0% | |
| CCCTTGAAGAAGCTTTACAA+AGG | + | Chr7:63250868-63250887 | MsG0780039386.01.T01:CDS | 40.0% | |
| CCCTTTGTAAAGCTTCTTCA+AGG | - | Chr7:63250872-63250891 | None:intergenic | 40.0% | |
| CTTGCAGTCTTAATCTGGTT+TGG | - | Chr7:63250375-63250394 | None:intergenic | 40.0% | |
| GATACTAATGGAATTGCAGC+AGG | + | Chr7:63250188-63250207 | MsG0780039386.01.T01:CDS | 40.0% | |
| GGCATAGAACATTCCTTCAT+GGG | - | Chr7:63250144-63250163 | None:intergenic | 40.0% | |
| TCAAGTCTGGAGAAGATACA+CGG | - | Chr7:63250170-63250189 | None:intergenic | 40.0% | |
| TGAAGCAAATTCTTCGCAGA+TGG | + | Chr7:63250792-63250811 | MsG0780039386.01.T01:intron | 40.0% | |
| TGATAAGAACTGCCGAAATC+AGG | + | Chr7:63250544-63250563 | MsG0780039386.01.T01:CDS | 40.0% | |
| TTAAGACTGCAAGTGGCAAA+AGG | + | Chr7:63250382-63250401 | MsG0780039386.01.T01:intron | 40.0% | |
| TTCAACAGTAGCTCGTACAT+CGG | - | Chr7:63250250-63250269 | None:intergenic | 40.0% | |
| ! | AGAGTGTATGCAAAAGCATG+AGG | + | Chr7:63250668-63250687 | MsG0780039386.01.T01:intron | 40.0% |
| ! | TGCTTTTGCATACACTCTTC+TGG | - | Chr7:63250666-63250685 | None:intergenic | 40.0% |
| AACCAAAGCCCACCTGATTT+CGG | - | Chr7:63250559-63250578 | None:intergenic | 45.0% | |
| AAGAACTGCCGAAATCAGGT+GGG | + | Chr7:63250548-63250567 | MsG0780039386.01.T01:CDS | 45.0% | |
| ACTCTTCTGGTGTACCTTCA+TGG | - | Chr7:63250653-63250672 | None:intergenic | 45.0% | |
| CAAGTCTGGAGAAGATACAC+GGG | - | Chr7:63250169-63250188 | None:intergenic | 45.0% | |
| TAAGAACTGCCGAAATCAGG+TGG | + | Chr7:63250547-63250566 | MsG0780039386.01.T01:CDS | 45.0% | |
| TACCCTGCAGAAATAACGCA+TGG | + | Chr7:63250622-63250641 | MsG0780039386.01.T01:CDS | 45.0% | |
| TGAGGTTCTTCAACCCATGA+AGG | + | Chr7:63250128-63250147 | MsG0780039386.01.T01:CDS | 45.0% | |
| ! | ATGGGTTGAAGAACCTCAGA+CGG | - | Chr7:63250126-63250145 | None:intergenic | 45.0% |
| ! | TGCCACTTGCAGTCTTAATC+TGG | - | Chr7:63250380-63250399 | None:intergenic | 45.0% |
| !! | AGACCATGCGTTATTTCTGC+AGG | - | Chr7:63250628-63250647 | None:intergenic | 45.0% |
| !! | GACCATGCGTTATTTCTGCA+GGG | - | Chr7:63250627-63250646 | None:intergenic | 45.0% |
| AAACCAATCGAGTCCGTCTG+AGG | + | Chr7:63250110-63250129 | MsG0780039386.01.T01:CDS | 50.0% | |
| AACGCATGGTCTAGCCATGA+AGG | + | Chr7:63250636-63250655 | MsG0780039386.01.T01:CDS | 50.0% | |
| CACTTGAGAAAACCCGTGAG+AGG | + | Chr7:63250304-63250323 | MsG0780039386.01.T01:CDS | 50.0% | |
| CTCGCCTTTGAACTCATCGA+TGG | - | Chr7:63250447-63250466 | None:intergenic | 50.0% | |
| GGTTGCGGCAAGGAAAATGA+AGG | + | Chr7:63250575-63250594 | MsG0780039386.01.T01:CDS | 50.0% | |
| TCTGGAGAAGATACACGGGA+AGG | - | Chr7:63250165-63250184 | None:intergenic | 50.0% | |
| TTCTTCAAGGGCCTTCTTGC+AGG | - | Chr7:63250859-63250878 | None:intergenic | 50.0% | |
| ! | AATCAGGTGGGCTTTGGTTG+CGG | + | Chr7:63250560-63250579 | MsG0780039386.01.T01:CDS | 50.0% |
| ! | TCTGACTCGTAAAGCTCGAG+AGG | + | Chr7:63250721-63250740 | MsG0780039386.01.T01:intron | 50.0% |
| AGGGTAGAGGCTGCAGATAG+AGG | + | Chr7:63250888-63250907 | MsG0780039386.01.T01:CDS | 55.0% | |
| GGGTAGAGGCTGCAGATAGA+GGG | + | Chr7:63250889-63250908 | MsG0780039386.01.T01:CDS | 55.0% | |
| TCGTAAAGCTCGAGAGGCAG+AGG | + | Chr7:63250727-63250746 | MsG0780039386.01.T01:intron | 55.0% | |
| ! | GAACCTCAGACGGACTCGAT+TGG | - | Chr7:63250116-63250135 | None:intergenic | 55.0% |
| ! | TGCCGAAATCAGGTGGGCTT+TGG | + | Chr7:63250554-63250573 | MsG0780039386.01.T01:CDS | 55.0% |
| !! | GTGGCAAAAGGTGCTGCTTC+TGG | + | Chr7:63250394-63250413 | MsG0780039386.01.T01:intron | 55.0% |
| ! | GGTGGGCTTTGGTTGCGGCA+AGG | + | Chr7:63250565-63250584 | MsG0780039386.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 63250018 | 63250935 | 63250018 | ID=MsG0780039386.01;Name=MsG0780039386.01 |
| Chr7 | mRNA | 63250018 | 63250935 | 63250018 | ID=MsG0780039386.01.T01;Parent=MsG0780039386.01;Name=MsG0780039386.01.T01;_AED=0.46;_eAED=0.46;_QI=0|0|0|1|1|1|3|0|219 |
| Chr7 | exon | 63250018 | 63250325 | 63250018 | ID=MsG0780039386.01.T01:exon:16005;Parent=MsG0780039386.01.T01 |
| Chr7 | exon | 63250428 | 63250670 | 63250428 | ID=MsG0780039386.01.T01:exon:16004;Parent=MsG0780039386.01.T01 |
| Chr7 | exon | 63250827 | 63250935 | 63250827 | ID=MsG0780039386.01.T01:exon:16003;Parent=MsG0780039386.01.T01 |
| Chr7 | CDS | 63250018 | 63250325 | 63250018 | ID=MsG0780039386.01.T01:cds;Parent=MsG0780039386.01.T01 |
| Chr7 | CDS | 63250428 | 63250670 | 63250428 | ID=MsG0780039386.01.T01:cds;Parent=MsG0780039386.01.T01 |
| Chr7 | CDS | 63250827 | 63250935 | 63250827 | ID=MsG0780039386.01.T01:cds;Parent=MsG0780039386.01.T01 |
| Gene Sequence |
| Protein sequence |
