Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016970.01.T01 | XP_003602787.1 | 96.296 | 540 | 20 | 0 | 1 | 540 | 1 | 540 | 0 | 1070 |
| MsG0380016970.01.T02 | XP_003602787.1 | 96.296 | 540 | 20 | 0 | 1 | 540 | 1 | 540 | 0 | 1070 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047814.01 | MsG0380016970.01 | 0.824714 | 6.950371e-54 | 6.206282e-51 |
| MsG0080047869.01 | MsG0380016970.01 | 0.800157 | 1.644328e-48 | 7.601643e-46 |
| MsG0080047944.01 | MsG0380016970.01 | 0.807097 | 5.970187e-50 | 3.295842e-47 |
| MsG0080047957.01 | MsG0380016970.01 | 0.804813 | 1.803613e-49 | 9.390642e-47 |
| MsG0080048291.01 | MsG0380016970.01 | 0.807027 | 6.175639e-50 | 3.403234e-47 |
| MsG0080048383.01 | MsG0380016970.01 | 0.833577 | 4.924705e-56 | 5.694771e-53 |
| MsG0080048404.01 | MsG0380016970.01 | 0.812972 | 3.239483e-51 | 2.088809e-48 |
| MsG0080048461.01 | MsG0380016970.01 | 0.853117 | 2.954103e-61 | 6.313859e-58 |
| MsG0080048462.01 | MsG0380016970.01 | 0.859075 | 5.315791e-63 | 1.386268e-59 |
| MsG0080048618.01 | MsG0380016970.01 | 0.827913 | 1.203118e-54 | 1.177661e-51 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016970.01.T01 | MTR_3g099000 | 96.296 | 540 | 20 | 0 | 1 | 540 | 1 | 540 | 0.0 | 1070 |
| MsG0380016970.01.T01 | MTR_3g116120 | 27.757 | 526 | 261 | 11 | 9 | 523 | 50 | 467 | 8.54e-56 | 195 |
| MsG0380016970.01.T01 | MTR_5g085170 | 27.238 | 525 | 245 | 11 | 7 | 523 | 22 | 417 | 7.44e-50 | 177 |
| MsG0380016970.01.T01 | MTR_3g088480 | 27.072 | 543 | 325 | 15 | 15 | 525 | 170 | 673 | 2.00e-43 | 165 |
| MsG0380016970.01.T01 | MTR_4g069830 | 29.693 | 293 | 164 | 7 | 16 | 290 | 139 | 407 | 3.96e-30 | 125 |
| MsG0380016970.01.T01 | MTR_4g069830 | 29.693 | 293 | 164 | 7 | 16 | 290 | 139 | 407 | 4.11e-30 | 125 |
| MsG0380016970.01.T01 | MTR_6g053260 | 43.860 | 114 | 58 | 1 | 2 | 115 | 17 | 124 | 2.38e-22 | 101 |
| MsG0380016970.01.T02 | MTR_3g099000 | 96.296 | 540 | 20 | 0 | 1 | 540 | 1 | 540 | 0.0 | 1070 |
| MsG0380016970.01.T02 | MTR_3g116120 | 27.757 | 526 | 261 | 11 | 9 | 523 | 50 | 467 | 8.54e-56 | 195 |
| MsG0380016970.01.T02 | MTR_5g085170 | 27.238 | 525 | 245 | 11 | 7 | 523 | 22 | 417 | 7.44e-50 | 177 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016970.01.T01 | AT2G47620 | 49.353 | 541 | 234 | 11 | 1 | 534 | 1 | 508 | 7.87e-151 | 442 |
| MsG0380016970.01.T01 | AT2G47620 | 50.361 | 415 | 167 | 9 | 1 | 410 | 1 | 381 | 5.54e-120 | 360 |
| MsG0380016970.01.T01 | AT2G33610 | 26.145 | 524 | 282 | 10 | 7 | 527 | 39 | 460 | 5.19e-52 | 184 |
| MsG0380016970.01.T01 | AT1G21700 | 26.866 | 536 | 321 | 17 | 15 | 527 | 175 | 662 | 1.29e-46 | 174 |
| MsG0380016970.01.T01 | AT4G34430 | 27.027 | 296 | 177 | 4 | 15 | 295 | 144 | 415 | 2.80e-26 | 114 |
| MsG0380016970.01.T01 | AT4G34430 | 27.027 | 296 | 177 | 4 | 15 | 295 | 144 | 415 | 2.84e-26 | 114 |
| MsG0380016970.01.T01 | AT4G34430 | 27.027 | 296 | 177 | 4 | 15 | 295 | 144 | 415 | 2.90e-26 | 114 |
| MsG0380016970.01.T01 | AT4G34430 | 27.027 | 296 | 177 | 4 | 15 | 295 | 144 | 415 | 2.90e-26 | 114 |
| MsG0380016970.01.T02 | AT2G47620 | 49.353 | 541 | 234 | 11 | 1 | 534 | 1 | 508 | 7.87e-151 | 442 |
| MsG0380016970.01.T02 | AT2G47620 | 50.361 | 415 | 167 | 9 | 1 | 410 | 1 | 381 | 5.54e-120 | 360 |
Find 14 sgRNAs with CRISPR-Local
Find 449 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCACCTATTAATCAATCAG+TGG | 0.645738 | 3:+90131847 | None:intergenic |
| AGAAGTGTATAAACAAGAGT+CGG | 0.605297 | 3:+90131914 | None:intergenic |
| AGTGCTTACTTGAAGAGCTT+GGG | 0.458213 | 3:+90131703 | None:intergenic |
| AGTTCCACTGATTGATTAAT+AGG | 0.320826 | 3:-90131851 | MsG0380016970.01.T02:five_prime_UTR |
| CATGCTAAAATGCGGAGGAG+GGG | 0.592057 | 3:+90131776 | None:intergenic |
| CCATGCTAAAATGCGGAGGA+GGG | 0.565736 | 3:+90131775 | None:intergenic |
| CCTTCCATGCTAAAATGCGG+AGG | 0.671452 | 3:+90131771 | None:intergenic |
| CGAGTTCTGAATCTGAAATT+GGG | 0.442640 | 3:+90131739 | None:intergenic |
| CTTACTTGAAGAGCTTGGGA+TGG | 0.637522 | 3:+90131707 | None:intergenic |
| GAGCCTTCCATGCTAAAATG+CGG | 0.623345 | 3:+90131768 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAATGAAGTTGAGCAGAA+TGG | - | Chr3:90128684-90128703 | MsG0380016970.01.T02:intron | 30.0% | |
| AAATTTGCATTGCATGAAGA+AGG | + | Chr3:90128191-90128210 | None:intergenic | 30.0% | |
| AACTTAACATAGTGTTTGGT+TGG | - | Chr3:90127436-90127455 | MsG0380016970.01.T02:intron | 30.0% | |
| AATTTGCATTGCATGAAGAA+GGG | + | Chr3:90128190-90128209 | None:intergenic | 30.0% | |
| ACTTAACATAGTGTTTGGTT+GGG | - | Chr3:90127437-90127456 | MsG0380016970.01.T02:intron | 30.0% | |
| AGTTACAACAACGAAGAATA+TGG | + | Chr3:90130895-90130914 | None:intergenic | 30.0% | |
| ATCTATCAAGAAGGATTTAG+TGG | - | Chr3:90128894-90128913 | MsG0380016970.01.T02:intron | 30.0% | |
| ATGAAAATCCTTCAAAAAGG+CGG | - | Chr3:90128718-90128737 | MsG0380016970.01.T02:intron | 30.0% | |
| ATGTGTTAATTAACGGAGTT+AGG | + | Chr3:90126501-90126520 | None:intergenic | 30.0% | |
| ATTGCAAGCAAAATAGTACT+GGG | + | Chr3:90128075-90128094 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 90126198 | 90131942 | 90126198 | ID=MsG0380016970.01;Name=MsG0380016970.01 |
| Chr3 | mRNA | 90126198 | 90131942 | 90126198 | ID=MsG0380016970.01.T01;Parent=MsG0380016970.01;Name=MsG0380016970.01.T01;_AED=0.45;_eAED=0.45;_QI=132|1|1|1|0.85|0.75|8|207|540 |
| Chr3 | exon | 90131712 | 90131871 | 90131712 | ID=MsG0380016970.01.T01:exon:25309;Parent=MsG0380016970.01.T01 |
| Chr3 | exon | 90131904 | 90131942 | 90131904 | ID=MsG0380016970.01.T01:exon:25308;Parent=MsG0380016970.01.T01 |
| Chr3 | five_prime_UTR | 90131904 | 90131942 | 90131904 | ID=MsG0380016970.01.T01:five_prime_utr;Parent=MsG0380016970.01.T01 |
| Chr3 | five_prime_UTR | 90131779 | 90131871 | 90131779 | ID=MsG0380016970.01.T01:five_prime_utr;Parent=MsG0380016970.01.T01 |
| Chr3 | CDS | 90131712 | 90131778 | 90131712 | ID=MsG0380016970.01.T01:cds;Parent=MsG0380016970.01.T01 |
| Chr3 | CDS | 90131038 | 90131591 | 90131038 | ID=MsG0380016970.01.T01:cds;Parent=MsG0380016970.01.T01 |
| Chr3 | CDS | 90129355 | 90129846 | 90129355 | ID=MsG0380016970.01.T01:cds;Parent=MsG0380016970.01.T01 |
| Chr3 | CDS | 90128772 | 90128905 | 90128772 | ID=MsG0380016970.01.T01:cds;Parent=MsG0380016970.01.T01 |
| Chr3 | CDS | 90128599 | 90128648 | 90128599 | ID=MsG0380016970.01.T01:cds;Parent=MsG0380016970.01.T01 |
| Chr3 | CDS | 90126713 | 90126858 | 90126713 | ID=MsG0380016970.01.T01:cds;Parent=MsG0380016970.01.T01 |
| Chr3 | CDS | 90126405 | 90126584 | 90126405 | ID=MsG0380016970.01.T01:cds;Parent=MsG0380016970.01.T01 |
| Chr3 | three_prime_UTR | 90126198 | 90126404 | 90126198 | ID=MsG0380016970.01.T01:three_prime_utr;Parent=MsG0380016970.01.T01 |
| Chr3 | mRNA | 90126198 | 90131942 | 90126198 | ID=MsG0380016970.01.T02;Parent=MsG0380016970.01;Name=MsG0380016970.01.T02;_AED=0.45;_eAED=0.45;_QI=164|1|1|1|1|1|7|207|540 |
| Chr3 | exon | 90131712 | 90131942 | 90131712 | ID=MsG0380016970.01.T02:exon:25316;Parent=MsG0380016970.01.T02 |
| Chr3 | five_prime_UTR | 90131779 | 90131942 | 90131779 | ID=MsG0380016970.01.T02:five_prime_utr;Parent=MsG0380016970.01.T02 |
| Chr3 | CDS | 90131712 | 90131778 | 90131712 | ID=MsG0380016970.01.T02:cds;Parent=MsG0380016970.01.T02 |
| Chr3 | CDS | 90131038 | 90131591 | 90131038 | ID=MsG0380016970.01.T02:cds;Parent=MsG0380016970.01.T02 |
| Chr3 | CDS | 90129355 | 90129846 | 90129355 | ID=MsG0380016970.01.T02:cds;Parent=MsG0380016970.01.T02 |
| Chr3 | CDS | 90128772 | 90128905 | 90128772 | ID=MsG0380016970.01.T02:cds;Parent=MsG0380016970.01.T02 |
| Chr3 | CDS | 90128599 | 90128648 | 90128599 | ID=MsG0380016970.01.T02:cds;Parent=MsG0380016970.01.T02 |
| Chr3 | CDS | 90126713 | 90126858 | 90126713 | ID=MsG0380016970.01.T02:cds;Parent=MsG0380016970.01.T02 |
| Chr3 | CDS | 90126405 | 90126584 | 90126405 | ID=MsG0380016970.01.T02:cds;Parent=MsG0380016970.01.T02 |
| Chr3 | three_prime_UTR | 90126198 | 90126404 | 90126198 | ID=MsG0380016970.01.T02:three_prime_utr;Parent=MsG0380016970.01.T02 |
| Gene Sequence |
>MsG0380016970.01.T02 ATGGAAGGCTCAAAAGACCCAATTTCAGATTCAGAACTCGAACTCTACACCATCCCAAGCTCTTCAAAATGGTTCGCGTGGGACGAAATTCACGAAACAGAGAAAACCGCATTCAAAGAGTATTTCGACGGAACTTCAATAACACGAACACCCAAAATCTACAAAGAATATAGAGACTTCATCATCAACAAATACCGCGAAGAACCTTCTAGAAGGTTAACATTCACCGAGGTACGAAAATCACTCGTCGGTGACGTCACTTTCTTGAACAAGGTTTTTCTTTTCTTGGAGCGTTGGGGTTTGATTAACTACGGCGCGCCTTCTGCTGGCGACGGTGGGGAAGCGGAGAAGGAACATGAAGAGGAGCGGTGTAAGTTGAAGGTTGAGGAAGGTGCTCCTAATGGGATTCGTGTTGTTGCTACGCCAAATTCGTTGAAGCCTATTTCGTTGCCGCGGGATACGAAGATTGCTGATGGTGGTGGTGCTGGGAACGGGGTTGGTGTTAAGATTGCGCCGTTGGCGTCGTATTCAGATGTTTACGGTGATTTGATTCGACGTAAAGAGGTCAATTGTGGGAATTGTGGTGATAAATGTGGTTCTGGACATTACCGAAGTAGCAAGGATAATTTCATTATTTGCACGAAATGTTTCAAAAACGGGAATTATGGGGAGAAAAGGTCCATGGAGGATTTCAAATTAAATGAGTCAAGTGAAATTAGTGCCAACCACAGTGCTGTCTGGACTGAGGGAGAAACTCTTCTTCTTTTAGAATCTGTTTTGAAGCATGGGGATGACTGGGAACTTGTTGCTCAAAGTGTTCGAACCAAGACTAAACTTGAATGTATTTCAAAACTCATTGAGCTGCCCTTTGGGGAGCTCATGTTAGGCTCTGCTCGCCGAAATGATAACAGTAATAGTGTTACTGGCATTGTGAACAATGGAAACAAAGTTCAGGTATCTTCATCCGATCACCAAGAAACTTCAATGACACAGGATCAATCTTCTGAGCCTAAAAATGAAGTTGAGCAGAATGGAGATGCTGTGAATGAAAATCCTTCAAAAAGGCGGCGGGTTGCTGCACTTTCAGATTCCAGCAGTTCACTAATGAAGCAGGTGGGACTGCTCTCAACTGTGGTTGACCCTCATATCACAGCTGCTGCAGCTAGTGCTGCTATTACAGCACTTTGTGATGAGAATTCGCTCCCAAGGGATATATTTGATGTTGAAGAGGATAATGCTTCAGCAAGGGCTCTCGAGGCTGAAGGTTTAGAGTTGGTGGAGGGGTCCACTCAATCAGAAGTAAAAGATGACATACCATTGACACTACGTATAAGAGCTGCCATTGGAACGGCTCTTGGGGCTACTGCTGCACGAGCAAAGTTGTTGGCGGACCAAGAAGATAGAGAGATTGAACATTTAGTAGCAACTATAATTGATGCTCAGGTTGAGAAGTTGCAACAGAAGGTTAAACATTTTGATGAATTGGAGCTTTTGATGGAGAAGGAACACGCTGAAATGGAAGAGCTGAAAGATTCTATCTTAACTGAACGGATTGATGTGTTGTGGAAGACATTTAAATCTGGTGTCGCTAGATGGAAGCATTATCCTTCTCTAAAATCTTAG >MsG0380016970.01.T01 ATGGAAGGCTCAAAAGACCCAATTTCAGATTCAGAACTCGAACTCTACACCATCCCAAGCTCTTCAAAATGGTTCGCGTGGGACGAAATTCACGAAACAGAGAAAACCGCATTCAAAGAGTATTTCGACGGAACTTCAATAACACGAACACCCAAAATCTACAAAGAATATAGAGACTTCATCATCAACAAATACCGCGAAGAACCTTCTAGAAGGTTAACATTCACCGAGGTACGAAAATCACTCGTCGGTGACGTCACTTTCTTGAACAAGGTTTTTCTTTTCTTGGAGCGTTGGGGTTTGATTAACTACGGCGCGCCTTCTGCTGGCGACGGTGGGGAAGCGGAGAAGGAACATGAAGAGGAGCGGTGTAAGTTGAAGGTTGAGGAAGGTGCTCCTAATGGGATTCGTGTTGTTGCTACGCCAAATTCGTTGAAGCCTATTTCGTTGCCGCGGGATACGAAGATTGCTGATGGTGGTGGTGCTGGGAACGGGGTTGGTGTTAAGATTGCGCCGTTGGCGTCGTATTCAGATGTTTACGGTGATTTGATTCGACGTAAAGAGGTCAATTGTGGGAATTGTGGTGATAAATGTGGTTCTGGACATTACCGAAGTAGCAAGGATAATTTCATTATTTGCACGAAATGTTTCAAAAACGGGAATTATGGGGAGAAAAGGTCCATGGAGGATTTCAAATTAAATGAGTCAAGTGAAATTAGTGCCAACCACAGTGCTGTCTGGACTGAGGGAGAAACTCTTCTTCTTTTAGAATCTGTTTTGAAGCATGGGGATGACTGGGAACTTGTTGCTCAAAGTGTTCGAACCAAGACTAAACTTGAATGTATTTCAAAACTCATTGAGCTGCCCTTTGGGGAGCTCATGTTAGGCTCTGCTCGCCGAAATGATAACAGTAATAGTGTTACTGGCATTGTGAACAATGGAAACAAAGTTCAGGTATCTTCATCCGATCACCAAGAAACTTCAATGACACAGGATCAATCTTCTGAGCCTAAAAATGAAGTTGAGCAGAATGGAGATGCTGTGAATGAAAATCCTTCAAAAAGGCGGCGGGTTGCTGCACTTTCAGATTCCAGCAGTTCACTAATGAAGCAGGTGGGACTGCTCTCAACTGTGGTTGACCCTCATATCACAGCTGCTGCAGCTAGTGCTGCTATTACAGCACTTTGTGATGAGAATTCGCTCCCAAGGGATATATTTGATGTTGAAGAGGATAATGCTTCAGCAAGGGCTCTCGAGGCTGAAGGTTTAGAGTTGGTGGAGGGGTCCACTCAATCAGAAGTAAAAGATGACATACCATTGACACTACGTATAAGAGCTGCCATTGGAACGGCTCTTGGGGCTACTGCTGCACGAGCAAAGTTGTTGGCGGACCAAGAAGATAGAGAGATTGAACATTTAGTAGCAACTATAATTGATGCTCAGGTTGAGAAGTTGCAACAGAAGGTTAAACATTTTGATGAATTGGAGCTTTTGATGGAGAAGGAACACGCTGAAATGGAAGAGCTGAAAGATTCTATCTTAACTGAACGGATTGATGTGTTGTGGAAGACATTTAAATCTGGTGTCGCTAGATGGAAGCATTATCCTTCTCTAAAATCTTAG |
| Protein sequence |
>MsG0380016970.01.T02 MEGSKDPISDSELELYTIPSSSKWFAWDEIHETEKTAFKEYFDGTSITRTPKIYKEYRDFIINKYREEPSRRLTFTEVRKSLVGDVTFLNKVFLFLERWGLINYGAPSAGDGGEAEKEHEEERCKLKVEEGAPNGIRVVATPNSLKPISLPRDTKIADGGGAGNGVGVKIAPLASYSDVYGDLIRRKEVNCGNCGDKCGSGHYRSSKDNFIICTKCFKNGNYGEKRSMEDFKLNESSEISANHSAVWTEGETLLLLESVLKHGDDWELVAQSVRTKTKLECISKLIELPFGELMLGSARRNDNSNSVTGIVNNGNKVQVSSSDHQETSMTQDQSSEPKNEVEQNGDAVNENPSKRRRVAALSDSSSSLMKQVGLLSTVVDPHITAAAASAAITALCDENSLPRDIFDVEEDNASARALEAEGLELVEGSTQSEVKDDIPLTLRIRAAIGTALGATAARAKLLADQEDREIEHLVATIIDAQVEKLQQKVKHFDELELLMEKEHAEMEELKDSILTERIDVLWKTFKSGVARWKHYPSLKS* >MsG0380016970.01.T01 MEGSKDPISDSELELYTIPSSSKWFAWDEIHETEKTAFKEYFDGTSITRTPKIYKEYRDFIINKYREEPSRRLTFTEVRKSLVGDVTFLNKVFLFLERWGLINYGAPSAGDGGEAEKEHEEERCKLKVEEGAPNGIRVVATPNSLKPISLPRDTKIADGGGAGNGVGVKIAPLASYSDVYGDLIRRKEVNCGNCGDKCGSGHYRSSKDNFIICTKCFKNGNYGEKRSMEDFKLNESSEISANHSAVWTEGETLLLLESVLKHGDDWELVAQSVRTKTKLECISKLIELPFGELMLGSARRNDNSNSVTGIVNNGNKVQVSSSDHQETSMTQDQSSEPKNEVEQNGDAVNENPSKRRRVAALSDSSSSLMKQVGLLSTVVDPHITAAAASAAITALCDENSLPRDIFDVEEDNASARALEAEGLELVEGSTQSEVKDDIPLTLRIRAAIGTALGATAARAKLLADQEDREIEHLVATIIDAQVEKLQQKVKHFDELELLMEKEHAEMEELKDSILTERIDVLWKTFKSGVARWKHYPSLKS* |
