Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039489.01.T01 | XP_003623804.2 | 86.842 | 114 | 15 | 0 | 1 | 114 | 35 | 148 | 2.48E-57 | 198 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039489.01.T01 | Q9LE59 | 41.176 | 102 | 54 | 4 | 17 | 114 | 59 | 158 | 6.97E-13 | 67.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039489.01.T01 | G7L1F3 | 86.842 | 114 | 15 | 0 | 1 | 114 | 35 | 148 | 1.18e-57 | 198 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180005026.01 | MsG0780039489.01 | 0.809680 | 1.679002e-50 | 9.921688e-48 |
| MsG0480021241.01 | MsG0780039489.01 | 0.817784 | 2.755012e-52 | 2.025546e-49 |
| MsG0480022490.01 | MsG0780039489.01 | 0.810724 | 9.998794e-51 | 6.073413e-48 |
| MsG0480023188.01 | MsG0780039489.01 | 0.838281 | 3.157188e-57 | 4.207906e-54 |
| MsG0380016850.01 | MsG0780039489.01 | 0.844356 | 7.952115e-59 | 1.280461e-55 |
| MsG0380016871.01 | MsG0780039489.01 | 0.814365 | 1.598853e-51 | 1.070701e-48 |
| MsG0380016872.01 | MsG0780039489.01 | 0.800355 | 1.498646e-48 | 6.963466e-46 |
| MsG0380017430.01 | MsG0780039489.01 | 0.818045 | 2.405668e-52 | 1.781243e-49 |
| MsG0780039489.01 | MsG0780040049.01 | 0.801890 | 7.273131e-49 | 3.513530e-46 |
| MsG0780039489.01 | MsG0780040136.01 | 0.850088 | 2.128440e-60 | 4.121876e-57 |
| MsG0780039489.01 | MsG0880045022.01 | 0.814821 | 1.267502e-51 | 8.592996e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039489.01.T01 | MTR_7g075840 | 86.842 | 114 | 15 | 0 | 1 | 114 | 35 | 148 | 3.00e-61 | 198 |
| MsG0780039489.01.T01 | MTR_8g033360 | 66.327 | 98 | 32 | 1 | 18 | 114 | 18 | 115 | 5.66e-36 | 130 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039489.01.T01 | AT3G61130 | 41.176 | 102 | 54 | 4 | 17 | 114 | 59 | 158 | 7.11e-14 | 67.4 |
Find 30 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGCTTGTGCTTACCTCTTT+TGG | 0.165480 | 7:-64777018 | None:intergenic |
| TCTTGTCTAAGATGTGTTTC+TGG | 0.168293 | 7:-64777350 | None:intergenic |
| GAAATAATCTTTCTGCTTCT+TGG | 0.205764 | 7:+64777182 | MsG0780039489.01.T01:CDS |
| CCTATGTTGTTGGCTGTTGC+AGG | 0.258186 | 7:+64776945 | MsG0780039489.01.T01:CDS |
| ACCCTTCTGGCTAGCCTTAC+GGG | 0.301649 | 7:-64777508 | None:intergenic |
| CTGCTTCTTGGAGAGTTGTT+GGG | 0.314790 | 7:+64777194 | MsG0780039489.01.T01:CDS |
| GAGAAGGAAGGAAGATCTTC+AGG | 0.324973 | 7:+64777383 | MsG0780039489.01.T01:CDS |
| TCTGCTTCTTGGAGAGTTGT+TGG | 0.331359 | 7:+64777193 | MsG0780039489.01.T01:CDS |
| CACCCTTCTGGCTAGCCTTA+CGG | 0.339727 | 7:-64777509 | None:intergenic |
| TATGTCCAGCACGAATGATA+TGG | 0.354195 | 7:+64777135 | MsG0780039489.01.T01:CDS |
| TGCCTTCTGTTCTCTCCATT+TGG | 0.363246 | 7:-64776971 | None:intergenic |
| AAGAAGCAGAAAGATTATTT+CGG | 0.364907 | 7:-64777180 | None:intergenic |
| CTTGTCTAAGATGTGTTTCT+GGG | 0.373678 | 7:-64777349 | None:intergenic |
| AAGAATGATGCTCACCCTTC+TGG | 0.396798 | 7:-64777521 | None:intergenic |
| GACTACAAACGCAACAAATC+AGG | 0.423274 | 7:+64777222 | MsG0780039489.01.T01:CDS |
| AGAAGGGTGAGCATCATTCT+TGG | 0.428400 | 7:+64777523 | MsG0780039489.01.T01:CDS |
| ATGTCCAGCACGAATGATAT+GGG | 0.493141 | 7:+64777136 | MsG0780039489.01.T01:CDS |
| TCTTAGACAAGAAGTTCAAA+AGG | 0.513227 | 7:+64777361 | MsG0780039489.01.T01:CDS |
| CTGCAACAGCCAACAACATA+GGG | 0.515813 | 7:-64776944 | None:intergenic |
| CCTGCAACAGCCAACAACAT+AGG | 0.531038 | 7:-64776945 | None:intergenic |
| AGGTCCCATATCATTCGTGC+TGG | 0.538645 | 7:-64777140 | None:intergenic |
| ACAAGAAGTTCAAAAGGAGA+AGG | 0.565190 | 7:+64777367 | MsG0780039489.01.T01:CDS |
| TCCCGTAAGGCTAGCCAGAA+GGG | 0.568105 | 7:+64777507 | MsG0780039489.01.T01:CDS |
| GTCCCGTAAGGCTAGCCAGA+AGG | 0.574160 | 7:+64777506 | MsG0780039489.01.T01:CDS |
| GGCTGTTGCAGGCTACCAAA+TGG | 0.577386 | 7:+64776956 | MsG0780039489.01.T01:CDS |
| GAAGTTCAAAAGGAGAAGGA+AGG | 0.583365 | 7:+64777371 | MsG0780039489.01.T01:CDS |
| TACCAAATGGAGAGAACAGA+AGG | 0.593932 | 7:+64776969 | MsG0780039489.01.T01:CDS |
| ATGCAGCAGGTCTTCCTCAG+TGG | 0.620368 | 7:+64777476 | MsG0780039489.01.T01:intron |
| AGTGGACTGATAGTCCCGTA+AGG | 0.647693 | 7:+64777494 | MsG0780039489.01.T01:CDS |
| ACGGGACTATCAGTCCACTG+AGG | 0.806472 | 7:-64777490 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAATGAAAAATTAATGAGA+AGG | - | Chr7:64777457-64777476 | None:intergenic | 15.0% |
| ! | AACAACATAGGGTAAAAAAA+TGG | - | Chr7:64776936-64776955 | None:intergenic | 25.0% |
| ! | AAGAAGCAGAAAGATTATTT+CGG | - | Chr7:64777183-64777202 | None:intergenic | 25.0% |
| ! | ACATTAAAATCAGTAGCAAT+AGG | - | Chr7:64777308-64777327 | None:intergenic | 25.0% |
| ! | AGGTGAAATAAAATAAAGCA+TGG | - | Chr7:64777437-64777456 | None:intergenic | 25.0% |
| ! | GGTGAAATAAAATAAAGCAT+GGG | - | Chr7:64777436-64777455 | None:intergenic | 25.0% |
| ! | GTGAAATAAAATAAAGCATG+GGG | - | Chr7:64777435-64777454 | None:intergenic | 25.0% |
| ! | TCTAATCAATACCATATTGT+AGG | + | Chr7:64777099-64777118 | MsG0780039489.01.T01:intron | 25.0% |
| !! | TAAATCTCTTTTTCCAAAAG+AGG | + | Chr7:64777005-64777024 | MsG0780039489.01.T01:CDS | 25.0% |
| !! | TAATTTTTCATTAATGCAGC+AGG | + | Chr7:64777463-64777482 | MsG0780039489.01.T01:intron | 25.0% |
| !!! | ACAATATGGTATTGATTAGA+AGG | - | Chr7:64777099-64777118 | None:intergenic | 25.0% |
| !!! | ATTTTTTTACCCTATGTTGT+TGG | + | Chr7:64776935-64776954 | MsG0780039489.01.T01:CDS | 25.0% |
| !!! | CTTTTGGAAAAAGAGATTTA+AGG | - | Chr7:64777005-64777024 | None:intergenic | 25.0% |
| AACATCAAGAACCTACAATA+TGG | - | Chr7:64777113-64777132 | None:intergenic | 30.0% | |
| AATAGGACAATAATGCCTTT+CGG | - | Chr7:64777291-64777310 | None:intergenic | 30.0% | |
| GAAATAATCTTTCTGCTTCT+TGG | + | Chr7:64777182-64777201 | MsG0780039489.01.T01:CDS | 30.0% | |
| TCTTAGACAAGAAGTTCAAA+AGG | + | Chr7:64777361-64777380 | MsG0780039489.01.T01:CDS | 30.0% | |
| !! | ATGGTATTGATTAGAAGGTT+GGG | - | Chr7:64777094-64777113 | None:intergenic | 30.0% |
| !! | TATGGTATTGATTAGAAGGT+TGG | - | Chr7:64777095-64777114 | None:intergenic | 30.0% |
| ACAAGAAGTTCAAAAGGAGA+AGG | + | Chr7:64777367-64777386 | MsG0780039489.01.T01:CDS | 35.0% | |
| ATAGGACAATAATGCCTTTC+GGG | - | Chr7:64777290-64777309 | None:intergenic | 35.0% | |
| ! | CGGAAATTTTCTAGACTCAA+AGG | - | Chr7:64777163-64777182 | None:intergenic | 35.0% |
| ! | CTTGTCTAAGATGTGTTTCT+GGG | - | Chr7:64777352-64777371 | None:intergenic | 35.0% |
| ! | TCTTGTCTAAGATGTGTTTC+TGG | - | Chr7:64777353-64777372 | None:intergenic | 35.0% |
| ATGTCCAGCACGAATGATAT+GGG | + | Chr7:64777136-64777155 | MsG0780039489.01.T01:CDS | 40.0% | |
| CAAACGCAACAAATCAGGTT+AGG | + | Chr7:64777227-64777246 | MsG0780039489.01.T01:intron | 40.0% | |
| GAAGTTCAAAAGGAGAAGGA+AGG | + | Chr7:64777371-64777390 | MsG0780039489.01.T01:CDS | 40.0% | |
| GACTACAAACGCAACAAATC+AGG | + | Chr7:64777222-64777241 | MsG0780039489.01.T01:CDS | 40.0% | |
| TACCAAATGGAGAGAACAGA+AGG | + | Chr7:64776969-64776988 | MsG0780039489.01.T01:CDS | 40.0% | |
| TAGGACAATAATGCCTTTCG+GGG | - | Chr7:64777289-64777308 | None:intergenic | 40.0% | |
| TATGTCCAGCACGAATGATA+TGG | + | Chr7:64777135-64777154 | MsG0780039489.01.T01:CDS | 40.0% | |
| !! | GTATTGATTAGAAGGTTGGG+AGG | - | Chr7:64777091-64777110 | None:intergenic | 40.0% |
| AAGAATGATGCTCACCCTTC+TGG | - | Chr7:64777524-64777543 | None:intergenic | 45.0% | |
| AGAAGGGTGAGCATCATTCT+TGG | + | Chr7:64777523-64777542 | MsG0780039489.01.T01:CDS | 45.0% | |
| AGGACAATAATGCCTTTCGG+GGG | - | Chr7:64777288-64777307 | None:intergenic | 45.0% | |
| CTGCAACAGCCAACAACATA+GGG | - | Chr7:64776947-64776966 | None:intergenic | 45.0% | |
| CTGCTTCTTGGAGAGTTGTT+GGG | + | Chr7:64777194-64777213 | MsG0780039489.01.T01:CDS | 45.0% | |
| GAGAAGGAAGGAAGATCTTC+AGG | + | Chr7:64777383-64777402 | MsG0780039489.01.T01:CDS | 45.0% | |
| TCTGCTTCTTGGAGAGTTGT+TGG | + | Chr7:64777193-64777212 | MsG0780039489.01.T01:CDS | 45.0% | |
| TGCCTTCTGTTCTCTCCATT+TGG | - | Chr7:64776974-64776993 | None:intergenic | 45.0% | |
| ! | AAGTTTGATTTCCCCCCGAA+AGG | + | Chr7:64777273-64777292 | MsG0780039489.01.T01:intron | 45.0% |
| ! | CAGCTTGTGCTTACCTCTTT+TGG | - | Chr7:64777021-64777040 | None:intergenic | 45.0% |
| AGGTCCCATATCATTCGTGC+TGG | - | Chr7:64777143-64777162 | None:intergenic | 50.0% | |
| CCTGCAACAGCCAACAACAT+AGG | - | Chr7:64776948-64776967 | None:intergenic | 50.0% | |
| GGACAATAATGCCTTTCGGG+GGG | - | Chr7:64777287-64777306 | None:intergenic | 50.0% | |
| ! | CCTATGTTGTTGGCTGTTGC+AGG | + | Chr7:64776945-64776964 | MsG0780039489.01.T01:CDS | 50.0% |
| !! | AGTGGACTGATAGTCCCGTA+AGG | + | Chr7:64777494-64777513 | MsG0780039489.01.T01:CDS | 50.0% |
| ACGGGACTATCAGTCCACTG+AGG | - | Chr7:64777493-64777512 | None:intergenic | 55.0% | |
| ATGCAGCAGGTCTTCCTCAG+TGG | + | Chr7:64777476-64777495 | MsG0780039489.01.T01:intron | 55.0% | |
| GGCTGTTGCAGGCTACCAAA+TGG | + | Chr7:64776956-64776975 | MsG0780039489.01.T01:CDS | 55.0% | |
| ! | TCCCGTAAGGCTAGCCAGAA+GGG | + | Chr7:64777507-64777526 | MsG0780039489.01.T01:CDS | 55.0% |
| !! | ACCCTTCTGGCTAGCCTTAC+GGG | - | Chr7:64777511-64777530 | None:intergenic | 55.0% |
| !! | CACCCTTCTGGCTAGCCTTA+CGG | - | Chr7:64777512-64777531 | None:intergenic | 55.0% |
| ! | GTCCCGTAAGGCTAGCCAGA+AGG | + | Chr7:64777506-64777525 | MsG0780039489.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 64776916 | 64777567 | 64776916 | ID=MsG0780039489.01;Name=MsG0780039489.01 |
| Chr7 | mRNA | 64776916 | 64777567 | 64776916 | ID=MsG0780039489.01.T01;Parent=MsG0780039489.01;Name=MsG0780039489.01.T01;_AED=0.48;_eAED=0.53;_QI=0|1|0.5|1|0.66|0.75|4|0|126 |
| Chr7 | exon | 64776916 | 64777026 | 64776916 | ID=MsG0780039489.01.T01:exon:9983;Parent=MsG0780039489.01.T01 |
| Chr7 | exon | 64777121 | 64777243 | 64777121 | ID=MsG0780039489.01.T01:exon:9984;Parent=MsG0780039489.01.T01 |
| Chr7 | exon | 64777341 | 64777404 | 64777341 | ID=MsG0780039489.01.T01:exon:9985;Parent=MsG0780039489.01.T01 |
| Chr7 | exon | 64777485 | 64777567 | 64777485 | ID=MsG0780039489.01.T01:exon:9986;Parent=MsG0780039489.01.T01 |
| Chr7 | CDS | 64776916 | 64777026 | 64776916 | ID=MsG0780039489.01.T01:cds;Parent=MsG0780039489.01.T01 |
| Chr7 | CDS | 64777121 | 64777243 | 64777121 | ID=MsG0780039489.01.T01:cds;Parent=MsG0780039489.01.T01 |
| Chr7 | CDS | 64777341 | 64777404 | 64777341 | ID=MsG0780039489.01.T01:cds;Parent=MsG0780039489.01.T01 |
| Chr7 | CDS | 64777485 | 64777567 | 64777485 | ID=MsG0780039489.01.T01:cds;Parent=MsG0780039489.01.T01 |
| Gene Sequence |
| Protein sequence |
