AlfalfaGEDB | MsG0380017430.01

M. sativa cultivar ZhongmuNo.1 / MsG0380017430.01

KEGG: -
GO: -
PFAM: PF03151

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017430.01.T01	XP_003603415.1	99.091	220	2	0	1	220	12	231	4.55E-153	439

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017430.01.T01	Q3E6T0	85.909	220	31	0	1	220	12	231	4.44E-137	391

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017430.01.T01	G7JB38	99.091	220	2	0	1	220	12	231	2.17e-153	439

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048374.01	MsG0380017430.01	0.802282	6.041925e-49	2.947933e-46
MsG0180004456.01	MsG0380017430.01	0.824167	9.345627e-54	8.214351e-51
MsG0180005026.01	MsG0380017430.01	0.854840	9.414051e-62	2.130704e-58
MsG0180006094.01	MsG0380017430.01	0.810701	1.011230e-50	6.138631e-48
MsG0380015495.01	MsG0380017430.01	0.809721	1.644815e-50	9.730487e-48
MsG0380015518.01	MsG0380017430.01	0.806958	6.387875e-50	3.513831e-47
MsG0380016567.01	MsG0380017430.01	0.816628	5.013553e-52	3.570999e-49
MsG0380016850.01	MsG0380017430.01	0.846936	1.588043e-59	2.777401e-56
MsG0380016871.01	MsG0380017430.01	0.841814	3.782512e-58	5.623095e-55
MsG0380016872.01	MsG0380017430.01	0.814215	1.725754e-51	1.150941e-48
MsG0380016927.01	MsG0380017430.01	0.803316	3.695494e-49	1.851175e-46
MsG0380017430.01	MsG0380017997.01	0.804508	2.088427e-49	1.078702e-46
MsG0380017430.01	MsG0480021241.01	0.843072	1.754673e-58	2.713541e-55
MsG0380017430.01	MsG0480022398.01	0.814511	1.484423e-51	9.980462e-49
MsG0380017430.01	MsG0480022490.01	0.817982	2.485813e-52	1.837397e-49
MsG0380017430.01	MsG0480023188.01	0.852603	4.141801e-61	8.700507e-58
MsG0380017430.01	MsG0480023697.01	0.815939	7.148431e-52	4.996458e-49
MsG0380017430.01	MsG0580025730.01	0.818312	2.093282e-52	1.561442e-49
MsG0380017430.01	MsG0580029468.01	0.818711	1.699476e-52	1.281713e-49
MsG0380017430.01	MsG0780037301.01	0.816584	5.128885e-52	3.648701e-49
MsG0380017430.01	MsG0780037406.01	0.800837	1.194939e-48	5.620640e-46
MsG0380017430.01	MsG0780039489.01	0.818045	2.405668e-52	1.781243e-49
MsG0380017430.01	MsG0780039530.01	0.814286	1.664944e-51	1.112553e-48
MsG0380017430.01	MsG0780040027.01	0.842684	2.225241e-58	3.399781e-55
MsG0380017430.01	MsG0780040049.01	0.851895	6.585303e-61	1.352328e-57
MsG0380017430.01	MsG0780040136.01	0.876494	1.335649e-68	6.489730e-65
MsG0380017430.01	MsG0780040564.01	0.834244	3.352474e-56	3.955467e-53
MsG0380017430.01	MsG0780041439.01	0.810483	1.126885e-50	6.800860e-48
MsG0380017430.01	MsG0780041548.01	0.837988	3.754208e-57	4.960398e-54
MsG0380017430.01	MsG0880045963.01	0.803555	3.296716e-49	1.661765e-46
MsG0380017430.01	MsG0880047478.01	0.800035	1.740912e-48	8.023493e-46
MsG0280007419.01	MsG0380017430.01	0.817609	3.017265e-52	2.207652e-49
MsG0280009241.01	MsG0380017430.01	0.807281	5.456089e-50	3.026682e-47
MsG0280009386.01	MsG0380017430.01	0.810745	9.892747e-51	6.012469e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017430.01.T01	MTR_3g107470	99.091	220	2	0	1	220	12	231	5.51e-157	439
MsG0380017430.01.T01	MTR_0662s0010	84.234	222	35	0	1	222	8	229	3.10e-137	386
MsG0380017430.01.T01	MTR_4g114360	84.234	222	35	0	1	222	8	229	6.41e-137	388
MsG0380017430.01.T01	MTR_0011s0320	79.909	219	44	0	1	219	8	226	5.88e-128	365
MsG0380017430.01.T01	MTR_7g110380	49.533	214	108	0	2	215	10	223	1.54e-72	225
MsG0380017430.01.T01	MTR_4g123900	51.402	214	104	0	2	215	6	219	1.50e-69	215
MsG0380017430.01.T01	MTR_4g123900	51.402	214	104	0	2	215	6	219	5.70e-68	214
MsG0380017430.01.T01	MTR_2g070400	43.256	215	121	1	6	219	10	224	8.75e-58	186
MsG0380017430.01.T01	MTR_8g090130	33.971	209	128	6	11	215	16	218	1.68e-20	88.2
MsG0380017430.01.T01	MTR_5g081710	31.122	196	129	3	9	202	11	202	2.77e-20	87.4
MsG0380017430.01.T01	MTR_3g462070	28.821	229	150	4	5	224	8	232	5.58e-20	86.7
MsG0380017430.01.T01	MTR_4g018760	28.922	204	139	3	14	215	20	219	2.98e-18	82.0
MsG0380017430.01.T01	MTR_3g070000	30.622	209	134	5	11	217	29	228	3.42e-18	82.0
MsG0380017430.01.T01	MTR_4g101860	28.723	188	128	3	11	196	57	240	4.84e-17	79.0
MsG0380017430.01.T01	MTR_8g016090	30.049	203	135	4	3	200	165	365	2.20e-16	77.8
MsG0380017430.01.T01	MTR_8g016090	28.889	180	122	3	3	178	165	342	1.47e-12	66.2
MsG0380017430.01.T01	MTR_3g072380	22.843	197	148	2	14	210	22	214	7.89e-12	63.9
MsG0380017430.01.T01	MTR_1g030320	22.843	197	148	2	14	210	21	213	3.80e-11	62.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017430.01.T01	AT5G25400	85.909	220	31	0	1	220	12	231	4.53e-138	391
MsG0380017430.01.T01	AT4G32390	85.909	220	31	0	1	220	12	231	1.04e-137	390
MsG0380017430.01.T01	AT2G25520	84.545	220	34	0	1	220	12	231	1.18e-136	387
MsG0380017430.01.T01	AT5G11230	82.432	222	39	0	1	222	12	233	5.36e-134	380
MsG0380017430.01.T01	AT3G17430	49.533	214	108	0	2	215	7	220	1.52e-66	210
MsG0380017430.01.T01	AT3G17430	49.533	214	108	0	2	215	7	220	1.52e-66	210
MsG0380017430.01.T01	AT1G48230	50.237	211	105	0	2	212	7	217	1.97e-62	199
MsG0380017430.01.T01	AT3G14410	43.379	219	123	1	1	218	9	227	7.74e-59	189
MsG0380017430.01.T01	AT1G53660	41.232	211	123	1	1	210	11	221	6.07e-56	181
MsG0380017430.01.T01	AT1G53660	41.232	211	123	1	1	210	29	239	9.18e-56	181
MsG0380017430.01.T01	AT1G53660	42.708	192	109	1	7	197	35	226	3.94e-53	171
MsG0380017430.01.T01	AT1G53660	43.011	186	105	1	26	210	2	187	2.39e-52	171
MsG0380017430.01.T01	AT1G48230	48.611	144	74	0	69	212	1	144	1.48e-40	140
MsG0380017430.01.T01	AT1G53660	44.094	127	71	0	84	210	1	127	5.82e-33	119
MsG0380017430.01.T01	AT3G11320	30.928	194	128	3	11	202	15	204	6.47e-19	83.6
MsG0380017430.01.T01	AT5G05820	30.928	194	128	3	11	202	15	204	1.12e-18	83.2
MsG0380017430.01.T01	AT5G04160	29.064	203	138	3	2	202	7	205	3.29e-15	73.6
MsG0380017430.01.T01	AT3G10290	30.244	205	133	6	2	202	53	251	5.00e-15	73.2
MsG0380017430.01.T01	AT1G06470	27.778	198	138	4	3	196	71	267	1.68e-14	72.0
MsG0380017430.01.T01	AT1G06470	27.778	198	138	4	3	196	71	267	1.68e-14	72.0
MsG0380017430.01.T01	AT1G06470	27.778	198	138	4	3	196	71	267	1.68e-14	72.0
MsG0380017430.01.T01	AT1G06470	27.778	198	138	4	3	196	71	267	1.68e-14	72.0
MsG0380017430.01.T01	AT1G12500	28.372	215	144	7	5	215	57	265	9.54e-14	69.7
MsG0380017430.01.T01	AT1G21870	22.843	197	148	2	14	210	22	214	2.28e-11	62.8
MsG0380017430.01.T01	AT1G77610	22.335	197	149	2	14	210	16	208	2.28e-11	62.8

Find 43 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGGATCATTGTTAGGGAAAT+TGG	0.112906	3:-96204107	None:intergenic
CTTGAATTCTCTTTCAAAAT+TGG	0.124329	3:-96204626	None:intergenic
CACTCTATTCTCTTTCTCTT+TGG	0.210913	3:+96204240	MsG0380017430.01.T01:CDS
AAGACATGTGGATCATTGTT+AGG	0.332971	3:-96204115	None:intergenic
GGTGTTGCTGTTGCTGCTTA+TGG	0.337039	3:+96204413	MsG0380017430.01.T01:CDS
TAACATGCTTTCCATTTCAA+TGG	0.363837	3:+96204391	MsG0380017430.01.T01:CDS
CTCTCTTCCGTTGTTCCAAT+CGG	0.368130	3:+96204215	MsG0380017430.01.T01:CDS
CGTACACATACGTAGCGATA+TGG	0.372435	3:+96204012	MsG0380017430.01.T01:CDS
TTATGTTGCACCTTGTTGTT+TGG	0.401542	3:+96204568	MsG0380017430.01.T01:CDS
GGATCATTGTTAGGGAAATT+GGG	0.407611	3:-96204106	None:intergenic
CCTGTTGCTGTTTATTCCAT+CGG	0.411489	3:+96204326	MsG0380017430.01.T01:CDS
CGGATTCAATGAAATCCCTT+TGG	0.424060	3:-96204531	None:intergenic
ACATAATACAAGGACGTAAT+CGG	0.431146	3:-96204551	None:intergenic
AACAGCAACACCCATTGAAA+TGG	0.448245	3:-96204402	None:intergenic
CCGATGGAATAAACAGCAAC+AGG	0.456849	3:-96204326	None:intergenic
AACATGCTTTCCATTTCAAT+GGG	0.463522	3:+96204392	MsG0380017430.01.T01:CDS
GATTTGAATCATAACCAATC+TGG	0.477776	3:-96204498	None:intergenic
CAAATCTTGCTTACATCCAA+AGG	0.480386	3:+96204515	MsG0380017430.01.T01:CDS
GCTGTTTATTCCATCGGCGT+TGG	0.482745	3:+96204332	MsG0380017430.01.T01:CDS
AGACATGTGGATCATTGTTA+GGG	0.498510	3:-96204114	None:intergenic
AAATCTTGCTTACATCCAAA+GGG	0.503028	3:+96204516	MsG0380017430.01.T01:CDS
GTCTGTTCCTTGGATCCTTG+TGG	0.511759	3:+96204598	MsG0380017430.01.T01:CDS
ACATCGCGAGACATTGAAAC+GGG	0.524525	3:-96204188	None:intergenic
GTGAAGCTAGATTTGATACA+TGG	0.526661	3:+96204435	MsG0380017430.01.T01:CDS
TGCTTTCGAAGCGACCAGAT+TGG	0.532292	3:+96204484	MsG0380017430.01.T01:CDS
AGAGAATAGAGTGCTCCGAT+TGG	0.540437	3:-96204230	None:intergenic
ATTGTTAGGGAAATTGGGAA+TGG	0.577490	3:-96204101	None:intergenic
AACATCGCGAGACATTGAAA+CGG	0.579577	3:-96204189	None:intergenic
GGATACTCCACAAGGATCCA+AGG	0.583076	3:-96204605	None:intergenic
CACGAACGAGAAGAATAGCT+AGG	0.589686	3:-96204148	None:intergenic
ACAAGGTGCAACATAATACA+AGG	0.594824	3:-96204561	None:intergenic
GAGTGCTCCGATTGGAACAA+CGG	0.598951	3:-96204222	None:intergenic
AACAGAGAGGTAAATGTAAG+CGG	0.607999	3:-96204273	None:intergenic
TGAAGCTAGATTTGATACAT+GGG	0.631766	3:+96204436	MsG0380017430.01.T01:CDS
ACGAACGAGAAGAATAGCTA+GGG	0.640942	3:-96204147	None:intergenic
AATGGAAAGCATGTTAAACA+TGG	0.654067	3:-96204384	None:intergenic
AGCTAGATTTGATACATGGG+GGG	0.655581	3:+96204439	MsG0380017430.01.T01:CDS
TCAAAATTGGATACTCCACA+AGG	0.664363	3:-96204613	None:intergenic
GAAGCTAGATTTGATACATG+GGG	0.666201	3:+96204437	MsG0380017430.01.T01:CDS
AAGCTAGATTTGATACATGG+GGG	0.682157	3:+96204438	MsG0380017430.01.T01:CDS
GACAAGAAAACCAAACAACA+AGG	0.684649	3:-96204578	None:intergenic
TTTGAATAAAAGAAACAGAG+AGG	0.688795	3:-96204286	None:intergenic
GGGTTGCACAGAAAGACATG+TGG	0.690413	3:-96204127	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	CTTGAATTCTCTTTCAAAAT+TGG	-	Chr3:96204629-96204648	None:intergenic	25.0%
!	TAGACAAAAAGATGTACAAT+TGG	+	Chr3:96204078-96204097	MsG0380017430.01.T01:CDS	25.0%
!	TTTGAATAAAAGAAACAGAG+AGG	-	Chr3:96204289-96204308	None:intergenic	25.0%
	AAATCTTGCTTACATCCAAA+GGG	+	Chr3:96204516-96204535	MsG0380017430.01.T01:CDS	30.0%
	AACATGCTTTCCATTTCAAT+GGG	+	Chr3:96204392-96204411	MsG0380017430.01.T01:CDS	30.0%
	ACATAATACAAGGACGTAAT+CGG	-	Chr3:96204554-96204573	None:intergenic	30.0%
	GATTTGAATCATAACCAATC+TGG	-	Chr3:96204501-96204520	None:intergenic	30.0%
	TAACATGCTTTCCATTTCAA+TGG	+	Chr3:96204391-96204410	MsG0380017430.01.T01:CDS	30.0%
	TGAAGCTAGATTTGATACAT+GGG	+	Chr3:96204436-96204455	MsG0380017430.01.T01:CDS	30.0%
!	AATGGAAAGCATGTTAAACA+TGG	-	Chr3:96204387-96204406	None:intergenic	30.0%
	AACAGAGAGGTAAATGTAAG+CGG	-	Chr3:96204276-96204295	None:intergenic	35.0%
	AAGACATGTGGATCATTGTT+AGG	-	Chr3:96204118-96204137	None:intergenic	35.0%
	AAGCTAGATTTGATACATGG+GGG	+	Chr3:96204438-96204457	MsG0380017430.01.T01:CDS	35.0%
	ACAAGGTGCAACATAATACA+AGG	-	Chr3:96204564-96204583	None:intergenic	35.0%
	AGACATGTGGATCATTGTTA+GGG	-	Chr3:96204117-96204136	None:intergenic	35.0%
	ATTGTTAGGGAAATTGGGAA+TGG	-	Chr3:96204104-96204123	None:intergenic	35.0%
	CAAATCTTGCTTACATCCAA+AGG	+	Chr3:96204515-96204534	MsG0380017430.01.T01:CDS	35.0%
	CACTCTATTCTCTTTCTCTT+TGG	+	Chr3:96204240-96204259	MsG0380017430.01.T01:CDS	35.0%
	GAAGCTAGATTTGATACATG+GGG	+	Chr3:96204437-96204456	MsG0380017430.01.T01:CDS	35.0%
	GACAAGAAAACCAAACAACA+AGG	-	Chr3:96204581-96204600	None:intergenic	35.0%
	GGATCATTGTTAGGGAAATT+GGG	-	Chr3:96204109-96204128	None:intergenic	35.0%
	GTGAAGCTAGATTTGATACA+TGG	+	Chr3:96204435-96204454	MsG0380017430.01.T01:CDS	35.0%
	TCAAAATTGGATACTCCACA+AGG	-	Chr3:96204616-96204635	None:intergenic	35.0%
	TGGATCATTGTTAGGGAAAT+TGG	-	Chr3:96204110-96204129	None:intergenic	35.0%
!!	TTATGTTGCACCTTGTTGTT+TGG	+	Chr3:96204568-96204587	MsG0380017430.01.T01:CDS	35.0%
	AACAGCAACACCCATTGAAA+TGG	-	Chr3:96204405-96204424	None:intergenic	40.0%
	AACATCGCGAGACATTGAAA+CGG	-	Chr3:96204192-96204211	None:intergenic	40.0%
	ACGAACGAGAAGAATAGCTA+GGG	-	Chr3:96204150-96204169	None:intergenic	40.0%
	AGCTAGATTTGATACATGGG+GGG	+	Chr3:96204439-96204458	MsG0380017430.01.T01:CDS	40.0%
	CGGATTCAATGAAATCCCTT+TGG	-	Chr3:96204534-96204553	None:intergenic	40.0%
!	TGGTTTTCTTGTCTGTTCCT+TGG	+	Chr3:96204588-96204607	MsG0380017430.01.T01:CDS	40.0%
!!	CCTGTTGCTGTTTATTCCAT+CGG	+	Chr3:96204326-96204345	MsG0380017430.01.T01:CDS	40.0%
	ACATCGCGAGACATTGAAAC+GGG	-	Chr3:96204191-96204210	None:intergenic	45.0%
	CACGAACGAGAAGAATAGCT+AGG	-	Chr3:96204151-96204170	None:intergenic	45.0%
	CCGATGGAATAAACAGCAAC+AGG	-	Chr3:96204329-96204348	None:intergenic	45.0%
	CGTACACATACGTAGCGATA+TGG	+	Chr3:96204012-96204031	MsG0380017430.01.T01:CDS	45.0%
	CTCTCTTCCGTTGTTCCAAT+CGG	+	Chr3:96204215-96204234	MsG0380017430.01.T01:CDS	45.0%
!	AGAGAATAGAGTGCTCCGAT+TGG	-	Chr3:96204233-96204252	None:intergenic	45.0%
!!	TGGGGGGTTATTTTACAGCT+TGG	+	Chr3:96204455-96204474	MsG0380017430.01.T01:CDS	45.0%
	GAGTGCTCCGATTGGAACAA+CGG	-	Chr3:96204225-96204244	None:intergenic	50.0%
	GCTGTTTATTCCATCGGCGT+TGG	+	Chr3:96204332-96204351	MsG0380017430.01.T01:CDS	50.0%
	GGATACTCCACAAGGATCCA+AGG	-	Chr3:96204608-96204627	None:intergenic	50.0%
	GGGTTGCACAGAAAGACATG+TGG	-	Chr3:96204130-96204149	None:intergenic	50.0%
	GTCTGTTCCTTGGATCCTTG+TGG	+	Chr3:96204598-96204617	MsG0380017430.01.T01:CDS	50.0%
	TGCTTTCGAAGCGACCAGAT+TGG	+	Chr3:96204484-96204503	MsG0380017430.01.T01:CDS	50.0%
!	TTTTCTCAGACCAACGCCGA+TGG	-	Chr3:96204345-96204364	None:intergenic	50.0%
!!	GGTGTTGCTGTTGCTGCTTA+TGG	+	Chr3:96204413-96204432	MsG0380017430.01.T01:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	96203993	96204667	96203993	ID=MsG0380017430.01;Name=MsG0380017430.01
Chr3	mRNA	96203993	96204667	96203993	ID=MsG0380017430.01.T01;Parent=MsG0380017430.01;Name=MsG0380017430.01.T01;_AED=0.36;_eAED=0.36;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|224
Chr3	exon	96203993	96204667	96203993	ID=MsG0380017430.01.T01:exon:17427;Parent=MsG0380017430.01.T01
Chr3	CDS	96203993	96204667	96203993	ID=MsG0380017430.01.T01:cds;Parent=MsG0380017430.01.T01

Gene Sequence

>MsG0380017430.01.T01

ATGAAGAAGATCTTATTATCGTACACATACGTAGCGATATGGATCTTCTTATCATTCACTGTTATCGTTTACAACAAATACATCTTAGACAAAAAGATGTACAATTGGCCATTCCCAATTTCCCTAACAATGATCCACATGTCTTTCTGTGCAACCCTAGCTATTCTTCTCGTTCGTGTCTTCAAATTCGTTGAACCCGTTTCAATGTCTCGCGATGTTTATCTCTCTTCCGTTGTTCCAATCGGAGCACTCTATTCTCTTTCTCTTTGGCTCTCAAATTCCGCTTACATTTACCTCTCTGTTTCTTTTATTCAAATGCTTAAAGCTCTTATGCCTGTTGCTGTTTATTCCATCGGCGTTGGTCTGAGAAAAGAATCTTATAAGAATGATACCATGTTTAACATGCTTTCCATTTCAATGGGTGTTGCTGTTGCTGCTTATGGTGAAGCTAGATTTGATACATGGGGGGTTATTTTACAGCTTGGTGCTGTTGCTTTCGAAGCGACCAGATTGGTTATGATTCAAATCTTGCTTACATCCAAAGGGATTTCATTGAATCCGATTACGTCCTTGTATTATGTTGCACCTTGTTGTTTGGTTTTCTTGTCTGTTCCTTGGATCCTTGTGGAGTATCCAATTTTGAAAGAGAATTCAAGTTTCATTTTGATTTTGTGA

Protein sequence

>MsG0380017430.01.T01

MKKILLSYTYVAIWIFLSFTVIVYNKYILDKKMYNWPFPISLTMIHMSFCATLAILLVRVFKFVEPVSMSRDVYLSSVVPIGALYSLSLWLSNSAYIYLSVSFIQMLKALMPVAVYSIGVGLRKESYKNDTMFNMLSISMGVAVAAYGEARFDTWGVILQLGAVAFEATRLVMIQILLTSKGISLNPITSLYYVAPCCLVFLSVPWILVEYPILKENSSFILIL*