Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039621.01.T01 | AFK39699.1 | 85.294 | 68 | 10 | 0 | 1 | 68 | 33 | 100 | 1.41E-34 | 123 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039621.01.T01 | I3SHF7 | 85.294 | 68 | 10 | 0 | 1 | 68 | 33 | 100 | 6.76e-35 | 123 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048845.01 | MsG0780039621.01 | -0.803607 | 3.216409e-49 | 1.623369e-46 |
| MsG0580024976.01 | MsG0780039621.01 | 0.806440 | 8.217913e-50 | 4.460682e-47 |
| MsG0780039186.01 | MsG0780039621.01 | -0.806722 | 7.165802e-50 | 3.917796e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039621.01.T01 | MTR_7g076190 | 88.235 | 68 | 8 | 0 | 1 | 68 | 310 | 377 | 5.16e-28 | 103 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCACTGTGGGTTGACAGTT+GGG | 0.402967 | 7:-66789167 | None:intergenic |
| ACAAATTGTGAATATATTGC+GGG | 0.419896 | 7:+66789235 | MsG0780039621.01.T01:CDS |
| TATATTGCGGGCACAAGTAA+TGG | 0.450390 | 7:+66789247 | MsG0780039621.01.T01:CDS |
| AGTAGAAGCTATGGATGGTT+GGG | 0.465286 | 7:+66789271 | MsG0780039621.01.T01:CDS |
| GCAATATATTCACAATTTGT+CGG | 0.480660 | 7:-66789232 | None:intergenic |
| ATATTGCGGGCACAAGTAAT+GGG | 0.492153 | 7:+66789248 | MsG0780039621.01.T01:CDS |
| GACAAATTGTGAATATATTG+CGG | 0.530460 | 7:+66789234 | MsG0780039621.01.T01:CDS |
| GAGTAGAAGCTATGGATGGT+TGG | 0.535671 | 7:+66789270 | MsG0780039621.01.T01:CDS |
| AGTCACTGTGGGTTGACAGT+TGG | 0.550409 | 7:-66789168 | None:intergenic |
| AGTAATGGGAGTAGAAGCTA+TGG | 0.552734 | 7:+66789262 | MsG0780039621.01.T01:CDS |
| TGGGTTGACAGTTGGGCCCG+TGG | 0.582111 | 7:-66789160 | None:intergenic |
| ATGGGAGTAGAAGCTATGGA+TGG | 0.618739 | 7:+66789266 | MsG0780039621.01.T01:CDS |
| TTATACCAGCAAAGATCACA+AGG | 0.624193 | 7:-66789339 | None:intergenic |
| TTGTATTGAAGAGAAACCAC+GGG | 0.632803 | 7:+66789144 | MsG0780039621.01.T01:CDS |
| AGTGACTTCTGAAAATCAGT+CGG | 0.655308 | 7:+66789184 | MsG0780039621.01.T01:CDS |
| TTTGTATTGAAGAGAAACCA+CGG | 0.681235 | 7:+66789143 | MsG0780039621.01.T01:CDS |
| TATACCAGCAAAGATCACAA+GGG | 0.718787 | 7:-66789338 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACAAATTGTGAATATATTGC+GGG | + | Chr7:66789235-66789254 | MsG0780039621.01.T01:CDS | 25.0% |
| ! | GACAAATTGTGAATATATTG+CGG | + | Chr7:66789234-66789253 | MsG0780039621.01.T01:CDS | 25.0% |
| ! | GCAATATATTCACAATTTGT+CGG | - | Chr7:66789235-66789254 | None:intergenic | 25.0% |
| TTTGTATTGAAGAGAAACCA+CGG | + | Chr7:66789143-66789162 | MsG0780039621.01.T01:CDS | 30.0% | |
| TTGTATTGAAGAGAAACCAC+GGG | + | Chr7:66789144-66789163 | MsG0780039621.01.T01:CDS | 35.0% | |
| ! | AGTGACTTCTGAAAATCAGT+CGG | + | Chr7:66789184-66789203 | MsG0780039621.01.T01:CDS | 35.0% |
| ! | TGATTTTCAGAAGTCACTGT+GGG | - | Chr7:66789182-66789201 | None:intergenic | 35.0% |
| AGTAGAAGCTATGGATGGTT+GGG | + | Chr7:66789271-66789290 | MsG0780039621.01.T01:CDS | 40.0% | |
| ATATTGCGGGCACAAGTAAT+GGG | + | Chr7:66789248-66789267 | MsG0780039621.01.T01:CDS | 40.0% | |
| TATATTGCGGGCACAAGTAA+TGG | + | Chr7:66789247-66789266 | MsG0780039621.01.T01:CDS | 40.0% | |
| ! | AGTAATGGGAGTAGAAGCTA+TGG | + | Chr7:66789262-66789281 | MsG0780039621.01.T01:CDS | 40.0% |
| ! | CTGATTTTCAGAAGTCACTG+TGG | - | Chr7:66789183-66789202 | None:intergenic | 40.0% |
| ATGGGAGTAGAAGCTATGGA+TGG | + | Chr7:66789266-66789285 | MsG0780039621.01.T01:CDS | 45.0% | |
| GAGTAGAAGCTATGGATGGT+TGG | + | Chr7:66789270-66789289 | MsG0780039621.01.T01:CDS | 45.0% | |
| ! | AGTCACTGTGGGTTGACAGT+TGG | - | Chr7:66789171-66789190 | None:intergenic | 50.0% |
| ! | GTCACTGTGGGTTGACAGTT+GGG | - | Chr7:66789170-66789189 | None:intergenic | 50.0% |
| ! | TGGGTTGACAGTTGGGCCCG+TGG | - | Chr7:66789163-66789182 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 66789140 | 66789346 | 66789140 | ID=MsG0780039621.01;Name=MsG0780039621.01 |
| Chr7 | mRNA | 66789140 | 66789346 | 66789140 | ID=MsG0780039621.01.T01;Parent=MsG0780039621.01;Name=MsG0780039621.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|68 |
| Chr7 | exon | 66789140 | 66789346 | 66789140 | ID=MsG0780039621.01.T01:exon:13184;Parent=MsG0780039621.01.T01 |
| Chr7 | CDS | 66789140 | 66789346 | 66789140 | ID=MsG0780039621.01.T01:cds;Parent=MsG0780039621.01.T01 |
| Gene Sequence |
| Protein sequence |
