AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024976.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024976.01.T01 MTR_5g020580 62.733 161 23 4 1 124 256 416 1.24e-52 171
MsG0580024976.01.T01 MTR_8g077195 55.346 159 36 4 1 124 254 412 1.56e-44 149
MsG0580024976.01.T01 MTR_1g022370 53.020 149 45 3 1 124 274 422 9.96e-39 134
MsG0580024976.01.T01 MTR_8g077650 55.882 136 43 3 1 121 200 333 5.97e-38 130
MsG0580024976.01.T01 MTR_8g077620 44.928 138 53 4 1 115 247 384 6.50e-26 100
MsG0580024976.01.T01 MTR_1g088960 44.231 156 52 6 1 124 250 402 8.09e-26 100
MsG0580024976.01.T01 MTR_2g020280 41.667 156 57 5 1 124 141 294 1.22e-25 98.2
MsG0580024976.01.T01 MTR_2g020280 41.667 156 57 5 1 124 240 393 7.64e-25 97.4
MsG0580024976.01.T01 MTR_8g077675 42.667 150 53 4 1 117 246 395 1.56e-24 96.7
MsG0580024976.01.T01 MTR_2g007930 37.059 170 61 5 1 124 258 427 1.06e-21 89.0
MsG0580024976.01.T01 MTR_2g076200 60.000 70 27 1 56 124 302 371 7.34e-19 80.9
MsG0580024976.01.T01 MTR_1g098790 35.593 177 61 7 1 124 255 431 2.51e-17 76.6
MsG0580024976.01.T01 MTR_2g076210 51.282 78 36 2 49 124 290 367 1.32e-16 74.7
MsG0580024976.01.T01 MTR_8g077640 60.656 61 22 2 56 114 323 383 2.57e-13 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024976.01.T01 AT1G47270 46.667 150 53 5 1 124 240 388 9.09e-29 107
MsG0580024976.01.T01 AT1G47270 46.667 150 53 5 1 124 264 412 1.01e-28 108
MsG0580024976.01.T01 AT1G47270 46.667 150 53 5 1 124 265 413 1.09e-28 108
MsG0580024976.01.T01 AT2G18280 44.966 149 56 5 1 124 246 393 1.97e-27 104
MsG0580024976.01.T01 AT2G18280 44.966 149 56 5 1 124 246 393 1.97e-27 104
MsG0580024976.01.T01 AT2G47900 41.667 156 56 6 1 124 254 406 1.84e-23 93.6
MsG0580024976.01.T01 AT2G47900 41.667 156 56 6 1 124 254 406 1.84e-23 93.6
MsG0580024976.01.T01 AT2G47900 41.667 156 56 6 1 124 255 407 1.93e-23 93.6
MsG0580024976.01.T01 AT5G18680 40.000 150 64 5 1 124 148 297 2.29e-23 92.0
MsG0580024976.01.T01 AT1G25280 37.912 182 55 6 1 124 86 267 3.23e-23 91.3
MsG0580024976.01.T01 AT1G25280 37.912 182 55 6 1 124 86 267 3.23e-23 91.3
MsG0580024976.01.T01 AT5G18680 40.000 150 64 5 1 124 240 389 2.26e-22 90.5
MsG0580024976.01.T01 AT1G25280 37.912 182 55 6 1 124 264 445 3.33e-22 90.5
MsG0580024976.01.T01 AT1G43640 38.506 174 55 9 1 124 192 363 8.26e-19 80.5
MsG0580024976.01.T01 AT1G43640 37.791 172 59 7 1 124 258 429 3.03e-18 79.3
MsG0580024976.01.T01 AT1G53320 60.000 70 27 1 56 124 310 379 6.12e-18 78.2
MsG0580024976.01.T01 AT3G06380 37.662 154 62 5 1 124 139 288 1.39e-17 76.6
MsG0580024976.01.T01 AT3G06380 58.571 70 25 2 56 124 314 380 2.63e-16 73.9
MsG0580024976.01.T01 AT1G76900 34.359 195 56 6 1 124 262 455 8.74e-16 72.4
MsG0580024976.01.T01 AT1G76900 34.359 195 56 6 1 124 262 455 8.74e-16 72.4
MsG0580024976.01.T01 AT1G76900 34.359 195 56 6 1 124 262 455 8.74e-16 72.4

Find 29 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCATTGGATCTTTACCTTT+TGG 0.237917 5:+12424251 None:intergenic
CATGGATCTCATTCACATTC+TGG 0.253260 5:+12423904 None:intergenic
TTCAACCCTCGCAGAGTTGC+CGG 0.340317 5:-12424217 MsG0580024976.01.T01:intron
ATCTTGCAGTTTGGAAAGAT+TGG 0.342353 5:-12424009 MsG0580024976.01.T01:CDS
GAGAAGGTTATCTTGCAGTT+TGG 0.354935 5:-12424018 MsG0580024976.01.T01:CDS
TATACCTGTCTCAGCTGTTC+AGG 0.357164 5:-12424350 MsG0580024976.01.T01:CDS
TTGGAAAAGATATATTACTA+TGG 0.366287 5:-12423990 MsG0580024976.01.T01:CDS
ATAACCTTCTCTTGCTCTGC+AGG 0.415381 5:+12424030 None:intergenic
TGAATTGCTTAAATCTCCAT+TGG 0.436374 5:+12424236 None:intergenic
TGAGGAAAAGATGTAGGAGT+TGG 0.437219 5:+12424313 None:intergenic
GTAATTTGAGGAAAAGATGT+AGG 0.503684 5:+12424307 None:intergenic
CCTTCCTGAACAGCTGAGAC+AGG 0.514005 5:+12424346 None:intergenic
AGTTAAGAACTTTCAACTTG+TGG 0.534362 5:-12424082 MsG0580024976.01.T01:CDS
AAATGCTCATCAGTAATTTG+AGG 0.539761 5:+12424295 None:intergenic
CCTGTCTCAGCTGTTCAGGA+AGG 0.540458 5:-12424346 MsG0580024976.01.T01:CDS
CTGTCTCAGCTGTTCAGGAA+GGG 0.541956 5:-12424345 MsG0580024976.01.T01:CDS
GCATATCGCAAAGGCTTGGA+AGG 0.543599 5:+12423948 None:intergenic
TGTCTCAGCTGTTCAGGAAG+GGG 0.560697 5:-12424344 MsG0580024976.01.T01:CDS
GCCAAAAGGTAAAGATCCAA+TGG 0.564544 5:-12424252 MsG0580024976.01.T01:CDS
GTCTCAGCTGTTCAGGAAGG+GGG 0.566290 5:-12424343 MsG0580024976.01.T01:CDS
GCGTCAGCACCAGAGCCAAA+AGG 0.578551 5:-12424266 MsG0580024976.01.T01:CDS
TCAAGCATATCGCAAAGGCT+TGG 0.579509 5:+12423944 None:intergenic
CTGAAACCGGCAACTCTGCG+AGG 0.580516 5:+12424211 None:intergenic
TTCTCCTGCAGAGCAAGAGA+AGG 0.597006 5:-12424034 MsG0580024976.01.T01:CDS
TGAAACCGGCAACTCTGCGA+GGG 0.603997 5:+12424212 None:intergenic
GCAGGAGAAACATTATGAGA+TGG 0.604319 5:+12424048 None:intergenic
AAGGCTTGGAAGGCAGAGAG+TGG 0.609206 5:+12423958 None:intergenic
GCTGCTCAAGCATATCGCAA+AGG 0.610612 5:+12423939 None:intergenic
TTGGAAGGCAGAGAGTGGAA+CGG 0.703026 5:+12423963 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGGAAAAGATATATTACTA+TGG - Chr5:12424292-12424311 MsG0580024976.01.T01:CDS 20.0%
AAATGCTCATCAGTAATTTG+AGG + Chr5:12423990-12424009 None:intergenic 30.0%
AGTTAAGAACTTTCAACTTG+TGG - Chr5:12424200-12424219 MsG0580024976.01.T01:intron 30.0%
GTAATTTGAGGAAAAGATGT+AGG + Chr5:12423978-12423997 None:intergenic 30.0%
TGAATTGCTTAAATCTCCAT+TGG + Chr5:12424049-12424068 None:intergenic 30.0%
ATCTTGCAGTTTGGAAAGAT+TGG - Chr5:12424273-12424292 MsG0580024976.01.T01:CDS 35.0%
! TCCATTGGATCTTTACCTTT+TGG + Chr5:12424034-12424053 None:intergenic 35.0%
ATGCTGGTGCCTAAATTTCA+AGG - Chr5:12424159-12424178 MsG0580024976.01.T01:intron 40.0%
GAGAAGGTTATCTTGCAGTT+TGG - Chr5:12424264-12424283 MsG0580024976.01.T01:CDS 40.0%
GCAGGAGAAACATTATGAGA+TGG + Chr5:12424237-12424256 None:intergenic 40.0%
GCCAAAAGGTAAAGATCCAA+TGG - Chr5:12424030-12424049 MsG0580024976.01.T01:CDS 40.0%
TCAAAAACAAGTCTCCCAGA+TGG - Chr5:12424122-12424141 MsG0580024976.01.T01:intron 40.0%
TGAGGAAAAGATGTAGGAGT+TGG + Chr5:12423972-12423991 None:intergenic 40.0%
TGCTGGTGCCTAAATTTCAA+GGG - Chr5:12424160-12424179 MsG0580024976.01.T01:intron 40.0%
TTAACTGAAGCCACTGTACA+CGG + Chr5:12424186-12424205 None:intergenic 40.0%
TTGAGTACCAGAGACTCAAA+TGG + Chr5:12424106-12424125 None:intergenic 40.0%
ATAACCTTCTCTTGCTCTGC+AGG + Chr5:12424255-12424274 None:intergenic 45.0%
GTACACGGCCCTTGAAATTT+AGG + Chr5:12424171-12424190 None:intergenic 45.0%
TATACCTGTCTCAGCTGTTC+AGG - Chr5:12423932-12423951 MsG0580024976.01.T01:CDS 45.0%
TCAAGCATATCGCAAAGGCT+TGG + Chr5:12424341-12424360 None:intergenic 45.0%
!!! GGATCTTTACCTTTTGGCTC+TGG + Chr5:12424028-12424047 None:intergenic 45.0%
ATTTCAAGGGCCGTGTACAG+TGG - Chr5:12424173-12424192 MsG0580024976.01.T01:intron 50.0%
CTGTCTCAGCTGTTCAGGAA+GGG - Chr5:12423937-12423956 MsG0580024976.01.T01:CDS 50.0%
GCATATCGCAAAGGCTTGGA+AGG + Chr5:12424337-12424356 None:intergenic 50.0%
GCTGCTCAAGCATATCGCAA+AGG + Chr5:12424346-12424365 None:intergenic 50.0%
TGCAGCTGTTCATGCCATCT+GGG + Chr5:12424139-12424158 None:intergenic 50.0%
TGTCTCAGCTGTTCAGGAAG+GGG - Chr5:12423938-12423957 MsG0580024976.01.T01:CDS 50.0%
TTCTCCTGCAGAGCAAGAGA+AGG - Chr5:12424248-12424267 MsG0580024976.01.T01:CDS 50.0%
TTGCAGCTGTTCATGCCATC+TGG + Chr5:12424140-12424159 None:intergenic 50.0%
TTGGAAGGCAGAGAGTGGAA+CGG + Chr5:12424322-12424341 None:intergenic 50.0%
AAGGCTTGGAAGGCAGAGAG+TGG + Chr5:12424327-12424346 None:intergenic 55.0%
CCAAGGGCCATTTGAGTCTC+TGG - Chr5:12424096-12424115 MsG0580024976.01.T01:intron 55.0%
CCAGAGACTCAAATGGCCCT+TGG + Chr5:12424099-12424118 None:intergenic 55.0%
CCTGTCTCAGCTGTTCAGGA+AGG - Chr5:12423936-12423955 MsG0580024976.01.T01:CDS 55.0%
CCTTCCTGAACAGCTGAGAC+AGG + Chr5:12423939-12423958 None:intergenic 55.0%
GGCATGAACAGCTGCAATGC+TGG - Chr5:12424143-12424162 MsG0580024976.01.T01:intron 55.0%
GTCTCAGCTGTTCAGGAAGG+GGG - Chr5:12423939-12423958 MsG0580024976.01.T01:CDS 55.0%
TGAAACCGGCAACTCTGCGA+GGG + Chr5:12424073-12424092 None:intergenic 55.0%
TTCAACCCTCGCAGAGTTGC+CGG - Chr5:12424065-12424084 MsG0580024976.01.T01:CDS 55.0%
!! AGTTGCCGGTTTCAGAGCCA+AGG - Chr5:12424079-12424098 MsG0580024976.01.T01:CDS 55.0%
!! ATGGCCCTTGGCTCTGAAAC+CGG + Chr5:12424087-12424106 None:intergenic 55.0%
!! GTTGCCGGTTTCAGAGCCAA+GGG - Chr5:12424080-12424099 MsG0580024976.01.T01:CDS 55.0%
CTGAAACCGGCAACTCTGCG+AGG + Chr5:12424074-12424093 None:intergenic 60.0%
GCGTCAGCACCAGAGCCAAA+AGG - Chr5:12424016-12424035 MsG0580024976.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 12423912 12424392 12423912 ID=MsG0580024976.01;Name=MsG0580024976.01
Chr5 mRNA 12423912 12424392 12423912 ID=MsG0580024976.01.T01;Parent=MsG0580024976.01;Name=MsG0580024976.01.T01;_AED=0.43;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|124
Chr5 exon 12424218 12424392 12424218 ID=MsG0580024976.01.T01:exon:19912;Parent=MsG0580024976.01.T01
Chr5 exon 12423912 12424111 12423912 ID=MsG0580024976.01.T01:exon:19911;Parent=MsG0580024976.01.T01
Chr5 CDS 12424218 12424392 12424218 ID=MsG0580024976.01.T01:cds;Parent=MsG0580024976.01.T01
Chr5 CDS 12423912 12424111 12423912 ID=MsG0580024976.01.T01:cds;Parent=MsG0580024976.01.T01
Gene Sequence

>MsG0580024976.01.T01

ATGAATTGCATCATGAATTCTATACCTGTCTCAGCTGTTCAGGAAGGGGGCAATGCTCCAACTCCTACATCTTTTCCTCAAATTACTGATGAGCATTTTGTTCTGCGTCAGCACCAGAGCCAAAAGGTAAAGATCCAATGGAGATTTAAGCAATTCAACCCTCGCAGAGTTGCCGTGGCTTCAGTTAAGAACTTTCAACTTGTGGCTGCTGTTGAGCCATCTCATAATGTTTCTCCTGCAGAGCAAGAGAAGGTTATCTTGCAGTTTGGAAAGATTGGAAAAGATATATTACTATGGATTACCGTTCCACTCTCTGCCTTCCAAGCCTTTGCGATATGCTTGAGCAGCTTTGATACAAAACCAGAATGTGAATGA

Protein sequence

>MsG0580024976.01.T01

MNCIMNSIPVSAVQEGGNAPTPTSFPQITDEHFVLRQHQSQKVKIQWRFKQFNPRRVAVASVKNFQLVAAVEPSHNVSPAEQEKVILQFGKIGKDILLWITVPLSAFQAFAICLSSFDTKPECE*