AlfalfaGEDB | MsG0580024976.01

M. sativa cultivar ZhongmuNo.1 / MsG0580024976.01

KEGG: K19600
GO: -
PFAM: PF01167

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024976.01.T01	RYQ80166.1	64.748	139	33	3	1	124	239	376	4.03E-44	158

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024976.01.T01	Q6Z2G9	46.875	160	49	4	1	124	269	428	5.30E-32	119

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024976.01.T01	G7KEX1	62.733	161	23	4	1	124	256	416	4.90e-49	171

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047884.01	MsG0580024976.01	-0.808101	3.654095e-50	2.070878e-47
MsG0080048085.01	MsG0580024976.01	-0.804436	2.162299e-49	1.114807e-46
MsG0080048528.01	MsG0580024976.01	-0.840716	7.355233e-58	1.056764e-54
MsG0080048727.01	MsG0580024976.01	-0.814955	1.183405e-51	8.051919e-49
MsG0080048773.01	MsG0580024976.01	-0.804712	1.894025e-49	9.835108e-47
MsG0080048780.01	MsG0580024976.01	-0.854736	1.009029e-61	2.275819e-58
MsG0080048801.01	MsG0580024976.01	-0.861029	1.367135e-63	3.813912e-60
MsG0080048820.01	MsG0580024976.01	-0.860314	2.252057e-63	6.129655e-60
MsG0080048845.01	MsG0580024976.01	-0.911073	9.187727e-83	1.935206e-78
MsG0080048929.01	MsG0580024976.01	-0.813357	2.666729e-51	1.737688e-48

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024976.01.T01	MTR_5g020580	62.733	161	23	4	1	124	256	416	1.24e-52	171
MsG0580024976.01.T01	MTR_8g077195	55.346	159	36	4	1	124	254	412	1.56e-44	149
MsG0580024976.01.T01	MTR_1g022370	53.020	149	45	3	1	124	274	422	9.96e-39	134
MsG0580024976.01.T01	MTR_8g077650	55.882	136	43	3	1	121	200	333	5.97e-38	130
MsG0580024976.01.T01	MTR_8g077620	44.928	138	53	4	1	115	247	384	6.50e-26	100
MsG0580024976.01.T01	MTR_1g088960	44.231	156	52	6	1	124	250	402	8.09e-26	100
MsG0580024976.01.T01	MTR_2g020280	41.667	156	57	5	1	124	141	294	1.22e-25	98.2
MsG0580024976.01.T01	MTR_2g020280	41.667	156	57	5	1	124	240	393	7.64e-25	97.4
MsG0580024976.01.T01	MTR_8g077675	42.667	150	53	4	1	117	246	395	1.56e-24	96.7
MsG0580024976.01.T01	MTR_2g007930	37.059	170	61	5	1	124	258	427	1.06e-21	89.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024976.01.T01	AT1G47270	46.667	150	53	5	1	124	240	388	9.09e-29	107
MsG0580024976.01.T01	AT1G47270	46.667	150	53	5	1	124	264	412	1.01e-28	108
MsG0580024976.01.T01	AT1G47270	46.667	150	53	5	1	124	265	413	1.09e-28	108
MsG0580024976.01.T01	AT2G18280	44.966	149	56	5	1	124	246	393	1.97e-27	104
MsG0580024976.01.T01	AT2G18280	44.966	149	56	5	1	124	246	393	1.97e-27	104
MsG0580024976.01.T01	AT2G47900	41.667	156	56	6	1	124	254	406	1.84e-23	93.6
MsG0580024976.01.T01	AT2G47900	41.667	156	56	6	1	124	254	406	1.84e-23	93.6
MsG0580024976.01.T01	AT2G47900	41.667	156	56	6	1	124	255	407	1.93e-23	93.6
MsG0580024976.01.T01	AT5G18680	40.000	150	64	5	1	124	148	297	2.29e-23	92.0
MsG0580024976.01.T01	AT1G25280	37.912	182	55	6	1	124	86	267	3.23e-23	91.3

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Find 29 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATGCTCATCAGTAATTTG+AGG	0.539761	5:+12424295	None:intergenic
AAGGCTTGGAAGGCAGAGAG+TGG	0.609206	5:+12423958	None:intergenic
AGTTAAGAACTTTCAACTTG+TGG	0.534362	5:-12424082	MsG0580024976.01.T01:CDS
ATAACCTTCTCTTGCTCTGC+AGG	0.415381	5:+12424030	None:intergenic
ATCTTGCAGTTTGGAAAGAT+TGG	0.342353	5:-12424009	MsG0580024976.01.T01:CDS
CATGGATCTCATTCACATTC+TGG	0.253260	5:+12423904	None:intergenic
CCTGTCTCAGCTGTTCAGGA+AGG	0.540458	5:-12424346	MsG0580024976.01.T01:CDS
CCTTCCTGAACAGCTGAGAC+AGG	0.514005	5:+12424346	None:intergenic
CTGAAACCGGCAACTCTGCG+AGG	0.580516	5:+12424211	None:intergenic
CTGTCTCAGCTGTTCAGGAA+GGG	0.541956	5:-12424345	MsG0580024976.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATGCTCATCAGTAATTTG+AGG	+	Chr5:12423990-12424009	None:intergenic	30.0%
AGTTAAGAACTTTCAACTTG+TGG	-	Chr5:12424200-12424219	MsG0580024976.01.T01:intron	30.0%
GTAATTTGAGGAAAAGATGT+AGG	+	Chr5:12423978-12423997	None:intergenic	30.0%
TGAATTGCTTAAATCTCCAT+TGG	+	Chr5:12424049-12424068	None:intergenic	30.0%
ATCTTGCAGTTTGGAAAGAT+TGG	-	Chr5:12424273-12424292	MsG0580024976.01.T01:CDS	35.0%
ATGCTGGTGCCTAAATTTCA+AGG	-	Chr5:12424159-12424178	MsG0580024976.01.T01:intron	40.0%
GAGAAGGTTATCTTGCAGTT+TGG	-	Chr5:12424264-12424283	MsG0580024976.01.T01:CDS	40.0%
GCAGGAGAAACATTATGAGA+TGG	+	Chr5:12424237-12424256	None:intergenic	40.0%
GCCAAAAGGTAAAGATCCAA+TGG	-	Chr5:12424030-12424049	MsG0580024976.01.T01:CDS	40.0%
TCAAAAACAAGTCTCCCAGA+TGG	-	Chr5:12424122-12424141	MsG0580024976.01.T01:intron	40.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	12423912	12424392	12423912	ID=MsG0580024976.01;Name=MsG0580024976.01
Chr5	mRNA	12423912	12424392	12423912	ID=MsG0580024976.01.T01;Parent=MsG0580024976.01;Name=MsG0580024976.01.T01;_AED=0.43;_eAED=0.50;_QI=0\|0\|0\|1\|1\|1\|2\|0\|124
Chr5	exon	12424218	12424392	12424218	ID=MsG0580024976.01.T01:exon:19912;Parent=MsG0580024976.01.T01
Chr5	exon	12423912	12424111	12423912	ID=MsG0580024976.01.T01:exon:19911;Parent=MsG0580024976.01.T01
Chr5	CDS	12424218	12424392	12424218	ID=MsG0580024976.01.T01:cds;Parent=MsG0580024976.01.T01
Chr5	CDS	12423912	12424111	12423912	ID=MsG0580024976.01.T01:cds;Parent=MsG0580024976.01.T01

Gene Sequence

>MsG0580024976.01.T01

ATGAATTGCATCATGAATTCTATACCTGTCTCAGCTGTTCAGGAAGGGGGCAATGCTCCAACTCCTACATCTTTTCCTCAAATTACTGATGAGCATTTTGTTCTGCGTCAGCACCAGAGCCAAAAGGTAAAGATCCAATGGAGATTTAAGCAATTCAACCCTCGCAGAGTTGCCGTGGCTTCAGTTAAGAACTTTCAACTTGTGGCTGCTGTTGAGCCATCTCATAATGTTTCTCCTGCAGAGCAAGAGAAGGTTATCTTGCAGTTTGGAAAGATTGGAAAAGATATATTACTATGGATTACCGTTCCACTCTCTGCCTTCCAAGCCTTTGCGATATGCTTGAGCAGCTTTGATACAAAACCAGAATGTGAATGA

Protein sequence

>MsG0580024976.01.T01

MNCIMNSIPVSAVQEGGNAPTPTSFPQITDEHFVLRQHQSQKVKIQWRFKQFNPRRVAVASVKNFQLVAAVEPSHNVSPAEQEKVILQFGKIGKDILLWITVPLSAFQAFAICLSSFDTKPECE*