AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040642.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040642.01.T01 MTR_7g094940 82.373 295 10 1 1 253 86 380 4.27e-168 469
MsG0780040642.01.T01 MTR_1g072190 58.621 290 70 5 1 246 86 369 1.18e-103 306
MsG0780040642.01.T01 MTR_1g095165 30.530 321 139 9 10 252 140 454 6.15e-36 133
MsG0780040642.01.T01 MTR_0368s0010 31.752 274 115 7 45 252 23 290 2.54e-33 122
MsG0780040642.01.T01 MTR_2g029510 33.333 279 116 11 9 233 134 396 7.12e-27 108
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040642.01.T01 AT3G63200 54.153 301 81 6 1 250 86 380 2.37e-104 308
MsG0780040642.01.T01 AT2G39220 29.245 318 154 7 1 252 165 477 7.11e-35 130
MsG0780040642.01.T01 AT3G54950 31.058 293 127 8 10 233 163 449 3.38e-30 117
MsG0780040642.01.T01 AT4G29800 30.968 310 136 9 1 233 178 486 8.22e-29 114
MsG0780040642.01.T01 AT4G29800 30.225 311 138 9 1 233 178 487 3.04e-27 109

Find 53 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGTTTCAACTTCGAGCTT+TGG 0.337029 7:-80513032 MsG0780040642.01.T01:CDS
CGCCGTTAGTCTCGTGCTAA+TGG 0.350594 7:-80512175 None:intergenic
TGTTGGGAAATGCTTTCAAT+TGG 0.372905 7:-80512700 MsG0780040642.01.T01:CDS
TTTGTGCAGGCATTTGGTTT+GGG 0.380361 7:-80512371 MsG0780040642.01.T01:intron
GGCATTTGGTTTGGGAGAAA+AGG 0.387921 7:-80512363 MsG0780040642.01.T01:CDS
TACTGACTATGCGAGGATTC+AGG 0.388689 7:-80512672 MsG0780040642.01.T01:intron
GTTTGGGAGAAAAGGGAAGT+TGG 0.393884 7:-80512355 MsG0780040642.01.T01:CDS
CAAAGCTCGAAGTTGAAACT+TGG 0.395282 7:+80513033 None:intergenic
GTGCAACGTCTTGTTGCTAC+TGG 0.398631 7:-80512233 MsG0780040642.01.T01:CDS
TTTGGGAGAAAAGGGAAGTT+GGG 0.449030 7:-80512354 MsG0780040642.01.T01:CDS
AAAGGGAAGTTGGGAAGAAA+CGG 0.458731 7:-80512345 MsG0780040642.01.T01:CDS
ATCACCACTGATGATGGATT+TGG 0.463331 7:-80513327 MsG0780040642.01.T01:CDS
CGCTGGTGCTCCGTTTCCGA+TGG 0.464766 7:+80512811 None:intergenic
CTGTTAATTTCATTGCTGAC+AGG 0.470929 7:-80513278 MsG0780040642.01.T01:CDS
GGGAAAATCGTTGACATTGA+AGG 0.471091 7:-80513142 MsG0780040642.01.T01:CDS
TACTCTTCGTTGAGAATCTC+CGG 0.471120 7:+80513208 None:intergenic
AGAAACCGTTGATCAGATGT+TGG 0.478228 7:-80512717 MsG0780040642.01.T01:CDS
GTATTGGAGTCGTTACCGTT+TGG 0.480768 7:-80512305 MsG0780040642.01.T01:CDS
CGTTACCGTTTGGTGGGAAA+CGG 0.481378 7:-80512295 MsG0780040642.01.T01:CDS
GAAACCGTTGATCAGATGTT+GGG 0.486925 7:-80512716 MsG0780040642.01.T01:CDS
GCATTTGGTTTGGGAGAAAA+GGG 0.487381 7:-80512362 MsG0780040642.01.T01:CDS
TTGGAGTCGTTACCGTTTGG+TGG 0.498381 7:-80512302 MsG0780040642.01.T01:CDS
GGTGTTGAATGAGAGAGTAT+TGG 0.507963 7:-80512321 MsG0780040642.01.T01:CDS
GCAATGATCACCACTGATGA+TGG 0.521349 7:-80513333 MsG0780040642.01.T01:CDS
TGGAGTCGTTACCGTTTGGT+GGG 0.535065 7:-80512301 MsG0780040642.01.T01:CDS
CACCATTAGCACGAGACTAA+CGG 0.541087 7:+80512173 None:intergenic
TGGAACGGCTTGAAGAGACT+TGG 0.566533 7:+80512982 None:intergenic
TCACGACGCATTCTGTTCGC+TGG 0.568343 7:+80512794 None:intergenic
AGTAACCGTTTCCCACCAAA+CGG 0.569382 7:+80512290 None:intergenic
GGCGTAATCTTGCATGGACT+AGG 0.570747 7:+80512194 None:intergenic
TCGTGAATGTTCAACGTCGA+CGG 0.577186 7:-80512768 MsG0780040642.01.T01:CDS
ACTAACGGCGTAATCTTGCA+TGG 0.577915 7:+80512188 None:intergenic
ATTTCCCAACATCTGATCAA+CGG 0.595899 7:+80512712 None:intergenic
GAATCCTCGCATAGTCAGTA+CGG 0.600379 7:+80512675 None:intergenic
TGAATGTTCAACGTCGACGG+TGG 0.606449 7:-80512765 MsG0780040642.01.T01:CDS
TCGACGGAAGCGAACTGGAA+CGG 0.607610 7:+80512967 None:intergenic
ACTTGGCGACTCCGACGCAT+CGG 0.614092 7:+80513050 None:intergenic
TTTCAAGGAAAAGAAAGCGA+GGG 0.614411 7:-80513162 MsG0780040642.01.T01:CDS
TCGTTGAGAATCTCCGGCGC+CGG 0.615276 7:+80513214 None:intergenic
GCATCTCTAGCAGTATAGAG+AGG 0.616286 7:+80513300 None:intergenic
GAATAGTGTTGAGGATCTGA+TGG 0.617697 7:-80512840 MsG0780040642.01.T01:intron
TTTCTCACGAGCCGATGCGT+CGG 0.628264 7:-80513061 MsG0780040642.01.T01:CDS
TCATAACAAGGAATAAGCAA+AGG 0.630651 7:+80513108 None:intergenic
GGGAAGTTGGGAAGAAACGG+AGG 0.634045 7:-80512342 MsG0780040642.01.T01:CDS
GTGGTTGACATTGCTCTCGA+CGG 0.634047 7:-80512746 MsG0780040642.01.T01:CDS
CTAACAGTAACAGAATTGAG+AGG 0.636540 7:-80512259 MsG0780040642.01.T01:CDS
CCCGTCGGTGAATAGTGTTG+AGG 0.647118 7:-80512849 MsG0780040642.01.T01:intron
GTAATCTTGCATGGACTAGG+TGG 0.653482 7:+80512197 None:intergenic
CGGCTTGAAGAGACTTGGAG+TGG 0.655997 7:+80512987 None:intergenic
TCGGCTCGTGAGAAAACGAA+CGG 0.678847 7:+80513069 None:intergenic
TCTACCAAATCCATCATCAG+TGG 0.680119 7:+80513323 None:intergenic
GGAACCGTACTGACTATGCG+AGG 0.687220 7:-80512679 MsG0780040642.01.T01:CDS
GAAGTCTTGAGATCATAACA+AGG 0.736456 7:+80513096 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGAATATAATCATATGTGT+CGG + Chr7:80512935-80512954 None:intergenic 20.0%
!!! CTCAAAGTGATTAATTTTAA+CGG - Chr7:80513095-80513114 MsG0780040642.01.T01:CDS 20.0%
!!! TTTTATAAAATGAAATCCGT+CGG - Chr7:80512262-80512281 MsG0780040642.01.T01:CDS 20.0%
! GATAGAAAGTAGTAAAAAAC+AGG - Chr7:80513052-80513071 MsG0780040642.01.T01:CDS 25.0%
!!! ATTTTAACGGAGTGAATTAA+CGG - Chr7:80513108-80513127 MsG0780040642.01.T01:CDS 25.0%
AGAAGCAAAAGATACAAACA+CGG + Chr7:80513001-80513020 None:intergenic 30.0%
TCATAACAAGGAATAAGCAA+AGG + Chr7:80512403-80512422 None:intergenic 30.0%
TGGAGAAAATTCACAACTAT+TGG + Chr7:80512869-80512888 None:intergenic 30.0%
ACGTCTTTCATCAGAAGTTA+TGG + Chr7:80512889-80512908 None:intergenic 35.0%
ATTTCCCAACATCTGATCAA+CGG + Chr7:80512799-80512818 None:intergenic 35.0%
CTAACAGTAACAGAATTGAG+AGG - Chr7:80513249-80513268 MsG0780040642.01.T01:CDS 35.0%
CTGTTAATTTCATTGCTGAC+AGG - Chr7:80512230-80512249 MsG0780040642.01.T01:CDS 35.0%
GAAGTCTTGAGATCATAACA+AGG + Chr7:80512415-80512434 None:intergenic 35.0%
TGTTGGGAAATGCTTTCAAT+TGG - Chr7:80512808-80512827 MsG0780040642.01.T01:CDS 35.0%
TTTCAAGGAAAAGAAAGCGA+GGG - Chr7:80512346-80512365 MsG0780040642.01.T01:CDS 35.0%
TTTCTGTCTAATGCACTGTT+TGG - Chr7:80513026-80513045 MsG0780040642.01.T01:CDS 35.0%
! TTGTTGAAGCGAGTTTTTCA+AGG - Chr7:80512331-80512350 MsG0780040642.01.T01:CDS 35.0%
!! TGTATTTTTGTGCAGGCATT+TGG - Chr7:80513131-80513150 MsG0780040642.01.T01:CDS 35.0%
!! TTAACGGTGTATTTTTGTGC+AGG - Chr7:80513124-80513143 MsG0780040642.01.T01:CDS 35.0%
!! TTTTCAAGGAAAAGAAAGCG+AGG - Chr7:80512345-80512364 MsG0780040642.01.T01:CDS 35.0%
AAAGGGAAGTTGGGAAGAAA+CGG - Chr7:80513163-80513182 MsG0780040642.01.T01:CDS 40.0%
AGAAACCGTTGATCAGATGT+TGG - Chr7:80512791-80512810 MsG0780040642.01.T01:CDS 40.0%
ATAACAACGTGATTTCCCGT+CGG - Chr7:80512644-80512663 MsG0780040642.01.T01:intron 40.0%
CAAAGCTCGAAGTTGAAACT+TGG + Chr7:80512478-80512497 None:intergenic 40.0%
CAAGTTTCAACTTCGAGCTT+TGG - Chr7:80512476-80512495 MsG0780040642.01.T01:intron 40.0%
GAAACCGTTGATCAGATGTT+GGG - Chr7:80512792-80512811 MsG0780040642.01.T01:CDS 40.0%
GGGAAAATCGTTGACATTGA+AGG - Chr7:80512366-80512385 MsG0780040642.01.T01:intron 40.0%
TACTCTTCGTTGAGAATCTC+CGG + Chr7:80512303-80512322 None:intergenic 40.0%
TCTACCAAATCCATCATCAG+TGG + Chr7:80512188-80512207 None:intergenic 40.0%
TTTGGGAGAAAAGGGAAGTT+GGG - Chr7:80513154-80513173 MsG0780040642.01.T01:CDS 40.0%
! GAATAGTGTTGAGGATCTGA+TGG - Chr7:80512668-80512687 MsG0780040642.01.T01:intron 40.0%
! GGTGTTGAATGAGAGAGTAT+TGG - Chr7:80513187-80513206 MsG0780040642.01.T01:CDS 40.0%
! GTGTCGGTACATAATCTTGT+TGG + Chr7:80512919-80512938 None:intergenic 40.0%
! TTTGTGCAGGCATTTGGTTT+GGG - Chr7:80513137-80513156 MsG0780040642.01.T01:CDS 40.0%
! TTTTGTGCAGGCATTTGGTT+TGG - Chr7:80513136-80513155 MsG0780040642.01.T01:CDS 40.0%
!! ATCACCACTGATGATGGATT+TGG - Chr7:80512181-80512200 MsG0780040642.01.T01:CDS 40.0%
!! GCATTTGGTTTGGGAGAAAA+GGG - Chr7:80513146-80513165 MsG0780040642.01.T01:CDS 40.0%
!! TGGTTTGGTGATGAACAATC+CGG - Chr7:80512594-80512613 MsG0780040642.01.T01:intron 40.0%
!!! ATGGTTTTGTCCATCGGAAA+CGG - Chr7:80512687-80512706 MsG0780040642.01.T01:CDS 40.0%
!!! GATCTGATGGTTTTGTCCAT+CGG - Chr7:80512681-80512700 MsG0780040642.01.T01:CDS 40.0%
ACTAACGGCGTAATCTTGCA+TGG + Chr7:80513323-80513342 None:intergenic 45.0%
AGTAACCGTTTCCCACCAAA+CGG + Chr7:80513221-80513240 None:intergenic 45.0%
CTTCTGATGAAAGACGTGTC+TGG - Chr7:80512892-80512911 MsG0780040642.01.T01:intron 45.0%
GAATCCTCGCATAGTCAGTA+CGG + Chr7:80512836-80512855 None:intergenic 45.0%
GCATCTCTAGCAGTATAGAG+AGG + Chr7:80512211-80512230 None:intergenic 45.0%
GTATTGGAGTCGTTACCGTT+TGG - Chr7:80513203-80513222 MsG0780040642.01.T01:CDS 45.0%
GTTTGGGAGAAAAGGGAAGT+TGG - Chr7:80513153-80513172 MsG0780040642.01.T01:CDS 45.0%
TCCTCAACACTATTCACCGA+CGG + Chr7:80512663-80512682 None:intergenic 45.0%
TCGTGAATGTTCAACGTCGA+CGG - Chr7:80512740-80512759 MsG0780040642.01.T01:CDS 45.0%
! TACTGACTATGCGAGGATTC+AGG - Chr7:80512836-80512855 MsG0780040642.01.T01:CDS 45.0%
!! GGCATTTGGTTTGGGAGAAA+AGG - Chr7:80513145-80513164 MsG0780040642.01.T01:CDS 45.0%
!! GTAATCTTGCATGGACTAGG+TGG + Chr7:80513314-80513333 None:intergenic 45.0%
AAAAACCTCATGCTCAGCCG+TGG - Chr7:80512567-80512586 MsG0780040642.01.T01:intron 50.0%
CCTCAACACTATTCACCGAC+GGG + Chr7:80512662-80512681 None:intergenic 50.0%
GGCGTAATCTTGCATGGACT+AGG + Chr7:80513317-80513336 None:intergenic 50.0%
GTGGTTGACATTGCTCTCGA+CGG - Chr7:80512762-80512781 MsG0780040642.01.T01:CDS 50.0%
TGAATGTTCAACGTCGACGG+TGG - Chr7:80512743-80512762 MsG0780040642.01.T01:CDS 50.0%
TGGAACGGCTTGAAGAGACT+TGG + Chr7:80512529-80512548 None:intergenic 50.0%
TGGAGTCGTTACCGTTTGGT+GGG - Chr7:80513207-80513226 MsG0780040642.01.T01:CDS 50.0%
TTGGAGTCGTTACCGTTTGG+TGG - Chr7:80513206-80513225 MsG0780040642.01.T01:CDS 50.0%
! CGTTACCGTTTGGTGGGAAA+CGG - Chr7:80513213-80513232 MsG0780040642.01.T01:CDS 50.0%
! GCATGAGGTTTTTCCGTCGA+CGG + Chr7:80512560-80512579 None:intergenic 50.0%
! GTGCAACGTCTTGTTGCTAC+TGG - Chr7:80513275-80513294 MsG0780040642.01.T01:CDS 50.0%
! TCGGCTCGTGAGAAAACGAA+CGG + Chr7:80512442-80512461 None:intergenic 50.0%
!! AATCCGTCGGTGTTTTTCGC+CGG - Chr7:80512275-80512294 MsG0780040642.01.T01:CDS 50.0%
CATCACCAAACCACCGTCCA+CGG + Chr7:80512587-80512606 None:intergenic 55.0%
CCCGTCGGTGAATAGTGTTG+AGG - Chr7:80512659-80512678 MsG0780040642.01.T01:intron 55.0%
CGGCTTGAAGAGACTTGGAG+TGG + Chr7:80512524-80512543 None:intergenic 55.0%
GATGAACAATCCGGCAGCAG+CGG - Chr7:80512603-80512622 MsG0780040642.01.T01:intron 55.0%
GGGAAGTTGGGAAGAAACGG+AGG - Chr7:80513166-80513185 MsG0780040642.01.T01:CDS 55.0%
TCACGACGCATTCTGTTCGC+TGG + Chr7:80512717-80512736 None:intergenic 55.0%
TCGACGGAAGCGAACTGGAA+CGG + Chr7:80512544-80512563 None:intergenic 55.0%
TTCCAGTTCGCTTCCGTCGA+CGG - Chr7:80512544-80512563 MsG0780040642.01.T01:intron 55.0%
TTTCTCACGAGCCGATGCGT+CGG - Chr7:80512447-80512466 MsG0780040642.01.T01:intron 55.0%
!! GGAACCGTACTGACTATGCG+AGG - Chr7:80512829-80512848 MsG0780040642.01.T01:CDS 55.0%
ACCTCATGCTCAGCCGTGGA+CGG - Chr7:80512571-80512590 MsG0780040642.01.T01:intron 60.0%
ACTTGGCGACTCCGACGCAT+CGG + Chr7:80512461-80512480 None:intergenic 60.0%
TCGTTGAGAATCTCCGGCGC+CGG + Chr7:80512297-80512316 None:intergenic 60.0%
TTCCGTCGACGGAAGCGAAC+TGG + Chr7:80512549-80512568 None:intergenic 60.0%
ACCGTCCACGGCTGAGCATG+AGG + Chr7:80512575-80512594 None:intergenic 65.0%
CGCTGGTGCTCCGTTTCCGA+TGG + Chr7:80512700-80512719 None:intergenic 65.0%
CTCAGCCGTGGACGGTGGTT+TGG - Chr7:80512579-80512598 MsG0780040642.01.T01:intron 65.0%
TCATGCTCAGCCGTGGACGG+TGG - Chr7:80512574-80512593 MsG0780040642.01.T01:intron 65.0%
TGCGTAACAGCCGCTGCTGC+CGG + Chr7:80512616-80512635 None:intergenic 65.0%
! GCGCCGGCGAAAAACACCGA+CGG + Chr7:80512281-80512300 None:intergenic 65.0%
!! TCGGTGTTTTTCGCCGGCGC+CGG - Chr7:80512281-80512300 MsG0780040642.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr7 gene 80512178 80513352 80512178 ID=MsG0780040642.01;Name=MsG0780040642.01
Chr7 mRNA 80512178 80513352 80512178 ID=MsG0780040642.01.T01;Parent=MsG0780040642.01;Name=MsG0780040642.01.T01;_AED=0.48;_eAED=0.49;_QI=0|0|0|1|1|1|3|0|253
Chr7 exon 80512982 80513352 80512982 ID=MsG0780040642.01.T01:exon:18604;Parent=MsG0780040642.01.T01
Chr7 exon 80512673 80512856 80512673 ID=MsG0780040642.01.T01:exon:18603;Parent=MsG0780040642.01.T01
Chr7 exon 80512178 80512384 80512178 ID=MsG0780040642.01.T01:exon:18602;Parent=MsG0780040642.01.T01
Chr7 CDS 80512982 80513352 80512982 ID=MsG0780040642.01.T01:cds;Parent=MsG0780040642.01.T01
Chr7 CDS 80512673 80512856 80512673 ID=MsG0780040642.01.T01:cds;Parent=MsG0780040642.01.T01
Chr7 CDS 80512178 80512384 80512178 ID=MsG0780040642.01.T01:cds;Parent=MsG0780040642.01.T01
Gene Sequence

>MsG0780040642.01.T01

ATGATCACCACTGATGATGGATTTGGTAGACCTCTCTATACTGCTAGAGATGCTGTTAATTTCATTGCTGACAGGAATCATGAATTTTATAAAATGAAATCCGTCGGTGTTTTTCGCCGGCGCCGGAGATTCTCAACGAAGAGTATTGAAAGTTTGTTGAAGCGAGTTTTTCAAGGAAAAGAAAGCGAGGGAAAATCGTTGACATTGAAGGATACGATTAAGCCTTTGCTTATTCCTTGTTATGATCTCAAGACTTCAGCACCGTTCGTTTTCTCACGAGCCGATGCGTCGGAGTCGCCAAGTTTCAACTTCGAGCTTTGGAAAGTGTGTCGCGCAACGTCGTCCACTCCAAGTCTCTTCAAGCCGTTCCATGTTGAGGATCTGATGGTTTTGTCCATCGGAAACGGAGCACCAGCGAACAGAATGCGTCGTGATGTTCGTGAATGTTCAACGTCGACGGTGGTTGACATTGCTCTCGACGGCGTTTCAGAAACCGTTGATCAGATGTTGGGAAATGCTTTCAATTGGAACCGTACTGACTATGCGAGGATTCAGGCATTTGGTTTGGGAGAAAAGGGAAGTTGGGAAGAAACGGAGGTGTTGAATGAGAGAGTATTGGAGTCGTTACCGTTTGGTGGGAAACGGTTACTACAAGAAACTAACAGTAACAGAATTGAGAGGTTTGTGCAACGTCTTGTTGCTACTGGAAAGTCTAGTTTGCCACCTAGTCCATGCAAGATTACGCCGTTAGTCTCGTGCTAA

Protein sequence

>MsG0780040642.01.T01

MITTDDGFGRPLYTARDAVNFIADRNHEFYKMKSVGVFRRRRRFSTKSIESLLKRVFQGKESEGKSLTLKDTIKPLLIPCYDLKTSAPFVFSRADASESPSFNFELWKVCRATSSTPSLFKPFHVEDLMVLSIGNGAPANRMRRDVRECSTSTVVDIALDGVSETVDQMLGNAFNWNRTDYARIQAFGLGEKGSWEETEVLNERVLESLPFGGKRLLQETNSNRIERFVQRLVATGKSSLPPSPCKITPLVSC*