Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041057.01.T01 | KEH24089.1 | 98.901 | 91 | 1 | 0 | 2 | 92 | 357 | 447 | 6.85E-51 | 176 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041057.01.T01 | Q8S9J0 | 76.596 | 94 | 17 | 1 | 2 | 90 | 354 | 447 | 2.86E-38 | 135 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041057.01.T01 | A0A072U313 | 98.901 | 91 | 1 | 0 | 2 | 92 | 357 | 447 | 3.27e-51 | 176 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003316.01 | MsG0780041057.01 | 0.807633 | 4.595023e-50 | 2.572324e-47 |
| MsG0780041057.01 | MsG0880043870.01 | 0.820288 | 7.424047e-53 | 5.849812e-50 |
| MsG0680035308.01 | MsG0780041057.01 | 0.809129 | 2.203944e-50 | 1.283416e-47 |
| MsG0480018848.01 | MsG0780041057.01 | 0.802577 | 5.251504e-49 | 2.581513e-46 |
| MsG0480023395.01 | MsG0780041057.01 | 0.831263 | 1.843207e-55 | 1.989668e-52 |
| MsG0380016762.01 | MsG0780041057.01 | 0.812497 | 4.115319e-51 | 2.620712e-48 |
| MsG0280010181.01 | MsG0780041057.01 | 0.805191 | 1.503895e-49 | 7.905957e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041057.01.T01 | MTR_7g103300 | 98.901 | 91 | 1 | 0 | 2 | 92 | 357 | 447 | 8.30e-55 | 176 |
| MsG0780041057.01.T01 | MTR_7g103300 | 100.000 | 80 | 0 | 0 | 2 | 81 | 267 | 346 | 9.81e-47 | 153 |
| MsG0780041057.01.T01 | MTR_7g103300 | 100.000 | 80 | 0 | 0 | 2 | 81 | 309 | 388 | 3.21e-46 | 152 |
| MsG0780041057.01.T01 | MTR_7g103300 | 100.000 | 80 | 0 | 0 | 2 | 81 | 357 | 436 | 5.97e-46 | 153 |
| MsG0780041057.01.T01 | MTR_1g061710 | 78.495 | 93 | 15 | 1 | 3 | 90 | 427 | 519 | 6.28e-39 | 135 |
| MsG0780041057.01.T01 | MTR_3g109950 | 63.529 | 85 | 31 | 0 | 2 | 86 | 364 | 448 | 2.44e-29 | 108 |
| MsG0780041057.01.T01 | MTR_3g109950 | 60.674 | 89 | 35 | 0 | 2 | 90 | 364 | 452 | 1.65e-28 | 106 |
| MsG0780041057.01.T01 | MTR_8g077830 | 62.500 | 80 | 30 | 0 | 2 | 81 | 363 | 442 | 1.44e-25 | 98.6 |
| MsG0780041057.01.T01 | MTR_8g088080 | 63.750 | 80 | 29 | 0 | 2 | 81 | 371 | 450 | 4.09e-25 | 97.4 |
| MsG0780041057.01.T01 | MTR_8g088070 | 62.500 | 80 | 30 | 0 | 2 | 81 | 366 | 445 | 2.14e-24 | 95.5 |
| MsG0780041057.01.T01 | MTR_5g019970 | 61.728 | 81 | 31 | 0 | 1 | 81 | 5 | 85 | 3.59e-23 | 85.9 |
| MsG0780041057.01.T01 | MTR_1g026910 | 53.086 | 81 | 38 | 0 | 2 | 82 | 353 | 433 | 5.39e-23 | 91.3 |
| MsG0780041057.01.T01 | MTR_1g026910 | 52.500 | 80 | 38 | 0 | 2 | 81 | 353 | 432 | 4.50e-22 | 89.0 |
| MsG0780041057.01.T01 | MTR_5g019950 | 60.000 | 80 | 32 | 0 | 2 | 81 | 366 | 445 | 2.69e-19 | 81.3 |
| MsG0780041057.01.T01 | MTR_7g103300 | 88.889 | 36 | 4 | 0 | 2 | 37 | 357 | 392 | 1.87e-14 | 67.4 |
| MsG0780041057.01.T01 | MTR_3g109950 | 78.788 | 33 | 7 | 0 | 2 | 34 | 364 | 396 | 2.23e-11 | 58.5 |
| MsG0780041057.01.T01 | MTR_3g110012 | 78.788 | 33 | 7 | 0 | 3 | 35 | 113 | 145 | 3.20e-11 | 56.2 |
| MsG0780041057.01.T01 | MTR_3g109980 | 78.788 | 33 | 7 | 0 | 3 | 35 | 113 | 145 | 3.20e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041057.01.T01 | AT2G36630 | 76.596 | 94 | 17 | 1 | 2 | 90 | 354 | 447 | 2.91e-39 | 135 |
| MsG0780041057.01.T01 | AT2G25737 | 63.750 | 80 | 29 | 0 | 2 | 81 | 370 | 449 | 3.10e-28 | 106 |
Find 15 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAAAATTGCTTAGAATTCT+TGG | 0.183484 | 7:+85940803 | MsG0780041057.01.T01:CDS |
| CCTCTTCACAGTGTCTGTTT+TGG | 0.327724 | 7:+85940754 | MsG0780041057.01.T01:CDS |
| AAGAGGTTCCCCATTCCTTA+TGG | 0.352277 | 7:+85940483 | MsG0780041057.01.T01:CDS |
| ATCATTGTATTCATCCTCTC+AGG | 0.365275 | 7:+85940836 | MsG0780041057.01.T01:CDS |
| TGAAAGGTACCATAAGGAAT+GGG | 0.440922 | 7:-85940492 | None:intergenic |
| ATGAAAGGTACCATAAGGAA+TGG | 0.503629 | 7:-85940493 | None:intergenic |
| TTTGCTAGTGCTCTCACCAT+GGG | 0.514375 | 7:+85940866 | MsG0780041057.01.T01:CDS |
| GAATGGGGAACCTCTTGAGC+AGG | 0.549756 | 7:-85940476 | None:intergenic |
| GAGGATGAATACAATGATTG+AGG | 0.567259 | 7:-85940831 | None:intergenic |
| TCATTGTATTCATCCTCTCA+GGG | 0.573524 | 7:+85940837 | MsG0780041057.01.T01:CDS |
| TTGAATTCTACCTGCTCAAG+AGG | 0.594537 | 7:+85940466 | MsG0780041057.01.T01:CDS |
| GAATCTGATTGATTTACCCA+TGG | 0.607905 | 7:-85940882 | None:intergenic |
| CCAAAACAGACACTGTGAAG+AGG | 0.614270 | 7:-85940754 | None:intergenic |
| GAAAGGTACCATAAGGAATG+GGG | 0.644081 | 7:-85940491 | None:intergenic |
| CATTGTATTCATCCTCTCAG+GGG | 0.714050 | 7:+85940838 | MsG0780041057.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGAAAATTGCTTAGAATTCT+TGG | + | Chr7:85940803-85940822 | MsG0780041057.01.T01:CDS | 25.0% |
| ! | AGGACTGTATAGAAATTAAT+AGG | + | Chr7:85940604-85940623 | MsG0780041057.01.T01:intron | 25.0% |
| ! | GAGAACTGAGAATTAATTAA+AGG | - | Chr7:85940557-85940576 | None:intergenic | 25.0% |
| ! | GTTGATTAGAAACAGATTTA+AGG | - | Chr7:85940530-85940549 | None:intergenic | 25.0% |
| CTGAGAATTAATTAAAGGCA+TGG | - | Chr7:85940552-85940571 | None:intergenic | 30.0% | |
| !! | AAGGTTTAAGAGTTGATGAA+AGG | - | Chr7:85940511-85940530 | None:intergenic | 30.0% |
| !! | ACTTTTTCTCTTACAAGACA+TGG | - | Chr7:85940581-85940600 | None:intergenic | 30.0% |
| ATCATTGTATTCATCCTCTC+AGG | + | Chr7:85940836-85940855 | MsG0780041057.01.T01:CDS | 35.0% | |
| CTTGTAAGAGAAAAAGTGAG+AGG | + | Chr7:85940584-85940603 | MsG0780041057.01.T01:intron | 35.0% | |
| GAGGATGAATACAATGATTG+AGG | - | Chr7:85940834-85940853 | None:intergenic | 35.0% | |
| TCATTGTATTCATCCTCTCA+GGG | + | Chr7:85940837-85940856 | MsG0780041057.01.T01:CDS | 35.0% | |
| ! | GAATCTGATTGATTTACCCA+TGG | - | Chr7:85940885-85940904 | None:intergenic | 35.0% |
| ! | GTTTTCATCATCATTGTCTG+TGG | + | Chr7:85940443-85940462 | MsG0780041057.01.T01:CDS | 35.0% |
| !! | AGTTGATGAAAGGTACCATA+AGG | - | Chr7:85940501-85940520 | None:intergenic | 35.0% |
| !! | ATGAAAGGTACCATAAGGAA+TGG | - | Chr7:85940496-85940515 | None:intergenic | 35.0% |
| !! | TGAAAGGTACCATAAGGAAT+GGG | - | Chr7:85940495-85940514 | None:intergenic | 35.0% |
| CATTGTATTCATCCTCTCAG+GGG | + | Chr7:85940838-85940857 | MsG0780041057.01.T01:CDS | 40.0% | |
| TTGAATTCTACCTGCTCAAG+AGG | + | Chr7:85940466-85940485 | MsG0780041057.01.T01:CDS | 40.0% | |
| !! | GAAAGGTACCATAAGGAATG+GGG | - | Chr7:85940494-85940513 | None:intergenic | 40.0% |
| AAGAGGTTCCCCATTCCTTA+TGG | + | Chr7:85940483-85940502 | MsG0780041057.01.T01:CDS | 45.0% | |
| CCAAAACAGACACTGTGAAG+AGG | - | Chr7:85940757-85940776 | None:intergenic | 45.0% | |
| TAGCAAAAATGACCCCTGAG+AGG | - | Chr7:85940853-85940872 | None:intergenic | 45.0% | |
| TTTGCTAGTGCTCTCACCAT+GGG | + | Chr7:85940866-85940885 | MsG0780041057.01.T01:CDS | 45.0% | |
| ! | CCTCTTCACAGTGTCTGTTT+TGG | + | Chr7:85940754-85940773 | MsG0780041057.01.T01:CDS | 45.0% |
| ! | TTTTGCTAGTGCTCTCACCA+TGG | + | Chr7:85940865-85940884 | MsG0780041057.01.T01:CDS | 45.0% |
| !!! | TCTGTTTTGGCTGGCTTTGT+GGG | + | Chr7:85940767-85940786 | MsG0780041057.01.T01:CDS | 45.0% |
| !!! | TTCACAGTGTCTGTTTTGGC+TGG | + | Chr7:85940758-85940777 | MsG0780041057.01.T01:CDS | 45.0% |
| !!! | GTCTGTTTTGGCTGGCTTTG+TGG | + | Chr7:85940766-85940785 | MsG0780041057.01.T01:CDS | 50.0% |
| GAATGGGGAACCTCTTGAGC+AGG | - | Chr7:85940479-85940498 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 85940405 | 85940934 | 85940405 | ID=MsG0780041057.01;Name=MsG0780041057.01 |
| Chr7 | mRNA | 85940405 | 85940934 | 85940405 | ID=MsG0780041057.01.T01;Parent=MsG0780041057.01;Name=MsG0780041057.01.T01;_AED=0.49;_eAED=0.52;_QI=0|1|0|1|1|1|2|0|95 |
| Chr7 | exon | 85940405 | 85940504 | 85940405 | ID=MsG0780041057.01.T01:exon:17924;Parent=MsG0780041057.01.T01 |
| Chr7 | exon | 85940747 | 85940934 | 85940747 | ID=MsG0780041057.01.T01:exon:17925;Parent=MsG0780041057.01.T01 |
| Chr7 | CDS | 85940405 | 85940504 | 85940405 | ID=MsG0780041057.01.T01:cds;Parent=MsG0780041057.01.T01 |
| Chr7 | CDS | 85940747 | 85940934 | 85940747 | ID=MsG0780041057.01.T01:cds;Parent=MsG0780041057.01.T01 |
| Gene Sequence |
| Protein sequence |
