AlfalfaGEDB | MsG0780041360.01

M. sativa cultivar ZhongmuNo.1 / MsG0780041360.01

KEGG: -
GO: GO:0003700 GO:0006355
PFAM: PF00170

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780041360.01.T01	XP_003625968.1	82.119	151	13	2	1	151	1	137	2.80E-70	218

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780041360.01.T01	Q9LZP8	46.711	152	70	3	1	149	1	144	1.06E-33	118

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780041360.01.T01	G7KST5	82.119	151	13	2	1	151	1	137	1.34e-70	218

TFs/TRs:

Gene ID	Type	Classification
MsG0780041360.01.T01	TF	bZIP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0780041360.01	MsG0880045869.01	0.826357	2.835382e-54	2.653046e-51
MsG0680034269.01	MsG0780041360.01	0.807462	4.996235e-50	2.784603e-47
MsG0480021240.01	MsG0780041360.01	0.811683	6.191569e-51	3.857308e-48
MsG0380016905.01	MsG0780041360.01	0.826772	2.258367e-54	2.138105e-51
MsG0780040125.01	MsG0780041360.01	0.816816	4.550579e-52	3.257953e-49

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780041360.01.T01	MTR_7g109510	82.119	151	13	2	1	151	1	137	3.39e-74	218
MsG0780041360.01.T01	MTR_1g087040	51.370	146	61	3	1	140	1	142	4.21e-39	129
MsG0780041360.01.T01	MTR_4g070860	48.387	93	47	1	10	101	17	109	1.11e-19	80.5
MsG0780041360.01.T01	MTR_3g117120	53.012	83	39	0	19	101	27	109	1.17e-19	80.5
MsG0780041360.01.T01	MTR_1g022495	54.217	83	38	0	19	101	27	109	1.75e-17	74.7
MsG0780041360.01.T01	MTR_8g086020	40.594	101	55	1	10	105	18	118	8.60e-16	70.5
MsG0780041360.01.T01	MTR_5g015090	52.857	70	24	1	2	62	32	101	6.59e-13	62.8
MsG0780041360.01.T01	MTR_4g097440	39.823	113	40	3	7	94	45	154	1.09e-11	60.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780041360.01.T01	AT3G62420	46.711	152	70	3	1	149	1	144	1.08e-34	118
MsG0780041360.01.T01	AT2G18160	44.118	102	52	1	18	119	27	123	1.11e-20	83.6
MsG0780041360.01.T01	AT1G75390	38.849	139	72	3	19	149	38	171	1.49e-20	83.2
MsG0780041360.01.T01	AT4G34590	48.810	84	43	0	19	102	24	107	1.51e-19	80.1
MsG0780041360.01.T01	AT1G75390	49.254	67	34	0	19	85	38	104	5.52e-12	59.7

Find 41 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGTCCGAATCAAGCGATTA+AGG	0.283521	7:+90030490	MsG0780041360.01.T01:CDS
GGCGATTGTTGGAGAACATT+AGG	0.296251	7:+90030267	MsG0780041360.01.T01:CDS
CAGATTGGTTCTTGCATTGA+TGG	0.301394	7:+90030525	MsG0780041360.01.T01:CDS
GGTTCAACGTGTGACGAGTT+CGG	0.348908	7:+90030097	MsG0780041360.01.T01:CDS
CACGGGTTCAGTTGCGGATC+TGG	0.352056	7:-90030458	None:intergenic
GAGCGATGGACGAGAGAAAA+AGG	0.360427	7:+90030138	MsG0780041360.01.T01:CDS
GTTCAACGTGTGACGAGTTC+GGG	0.369000	7:+90030098	MsG0780041360.01.T01:CDS
TGAATCAATTTGAATGGCTT+CGG	0.385700	7:+90030076	None:intergenic
GCGATTGTTGGAGAACATTA+GGG	0.409944	7:+90030268	MsG0780041360.01.T01:CDS
GTGATCGCCGTGCGGATTCA+CGG	0.422997	7:-90030178	None:intergenic
AAACGAGAATCGGCGATTGT+TGG	0.446153	7:+90030256	MsG0780041360.01.T01:CDS
AATGCAAACGAAGCCGCTAA+TGG	0.446683	7:+90030308	MsG0780041360.01.T01:CDS
ATCTTCAAGATGAGACAAAC+AGG	0.448219	7:+90030228	MsG0780041360.01.T01:CDS
AACATTAGGGCGAGGGAAGA+AGG	0.454886	7:+90030281	MsG0780041360.01.T01:CDS
ACGTGTGACGAGTTCGGGTT+CGG	0.460336	7:+90030103	MsG0780041360.01.T01:CDS
GACGCCTTAATCGCTTGATT+CGG	0.461919	7:-90030494	None:intergenic
AGTTGCGGATCTGGAATCTT+CGG	0.470204	7:-90030449	None:intergenic
AGGCGTCTTCGTCTTCAGAT+TGG	0.488511	7:+90030510	MsG0780041360.01.T01:CDS
TGTCCTCGGAGCTCGGATAC+AGG	0.488969	7:+90030331	MsG0780041360.01.T01:CDS
AAAGCTGAGAGGGAAAAGAA+TGG	0.490064	7:+90030410	MsG0780041360.01.T01:CDS
TCGGGTTCGGACGGTGTGAA+TGG	0.491849	7:+90030116	MsG0780041360.01.T01:CDS
GTGAATCCGCACGGCGATCA+CGG	0.512811	7:+90030180	MsG0780041360.01.T01:CDS
CTAATGGTGTCCTCGGAGCT+CGG	0.524737	7:+90030324	MsG0780041360.01.T01:CDS
TGAATCCGCACGGCGATCAC+GGG	0.531059	7:+90030181	MsG0780041360.01.T01:CDS
ACAGGTTGCGAAACGAGAAT+CGG	0.540937	7:+90030246	MsG0780041360.01.T01:CDS
CCGCACGGCGATCACGGGAG+AGG	0.550454	7:+90030186	MsG0780041360.01.T01:CDS
TGTTGGAGAACATTAGGGCG+AGG	0.554484	7:+90030273	MsG0780041360.01.T01:CDS
ATCTGCCACGGGTTCAGTTG+CGG	0.557161	7:-90030464	None:intergenic
AGCTCCGAGGACACCATTAG+CGG	0.564090	7:-90030321	None:intergenic
GAAGCCGCTAATGGTGTCCT+CGG	0.572523	7:+90030317	MsG0780041360.01.T01:CDS
TCGGACAAAGCATCTGCCAC+GGG	0.574386	7:-90030475	None:intergenic
GTTGGAGAACATTAGGGCGA+GGG	0.586730	7:+90030274	MsG0780041360.01.T01:CDS
GGAGAGGAAGCAGAAACTGA+TGG	0.591146	7:+90030202	MsG0780041360.01.T01:CDS
TTCGGACAAAGCATCTGCCA+CGG	0.597740	7:-90030476	None:intergenic
GGACGGTGTGAATGGAGCGA+TGG	0.618123	7:+90030124	MsG0780041360.01.T01:CDS
GTGACGAGTTCGGGTTCGGA+CGG	0.621958	7:+90030107	MsG0780041360.01.T01:CDS
CAGATCCGCAACTGAACCCG+TGG	0.634504	7:+90030459	MsG0780041360.01.T01:CDS
TTTCGAACCGTGAATCCGCA+CGG	0.677805	7:+90030171	MsG0780041360.01.T01:CDS
CCTCTCCCGTGATCGCCGTG+CGG	0.684095	7:-90030186	None:intergenic
GAGGAAGCAGAAACTGATGG+AGG	0.689697	7:+90030205	MsG0780041360.01.T01:CDS
ATTCCTGTATCCGAGCTCCG+AGG	0.746883	7:-90030334	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ATCTTCAAGATGAGACAAAC+AGG	+	Chr7:90030228-90030247	MsG0780041360.01.T01:CDS	35.0%
	AAAGCTGAGAGGGAAAAGAA+TGG	+	Chr7:90030410-90030429	MsG0780041360.01.T01:CDS	40.0%
	GCGATTGTTGGAGAACATTA+GGG	+	Chr7:90030268-90030287	MsG0780041360.01.T01:CDS	40.0%
	TGAGAGAGAAAAAGCTGAGA+GGG	+	Chr7:90030400-90030419	MsG0780041360.01.T01:CDS	40.0%
	TTGTCCGAATCAAGCGATTA+AGG	+	Chr7:90030490-90030509	MsG0780041360.01.T01:CDS	40.0%
	AAACGAGAATCGGCGATTGT+TGG	+	Chr7:90030256-90030275	MsG0780041360.01.T01:CDS	45.0%
	AATGCAAACGAAGCCGCTAA+TGG	+	Chr7:90030308-90030327	MsG0780041360.01.T01:CDS	45.0%
	ACAGGTTGCGAAACGAGAAT+CGG	+	Chr7:90030246-90030265	MsG0780041360.01.T01:CDS	45.0%
	CTGAGAGAGAAAAAGCTGAG+AGG	+	Chr7:90030399-90030418	MsG0780041360.01.T01:CDS	45.0%
	GACGCCTTAATCGCTTGATT+CGG	-	Chr7:90030497-90030516	None:intergenic	45.0%
	GGCGATTGTTGGAGAACATT+AGG	+	Chr7:90030267-90030286	MsG0780041360.01.T01:CDS	45.0%
!	AGTTGCGGATCTGGAATCTT+CGG	-	Chr7:90030452-90030471	None:intergenic	45.0%
	AACATTAGGGCGAGGGAAGA+AGG	+	Chr7:90030281-90030300	MsG0780041360.01.T01:CDS	50.0%
	AGGCGTCTTCGTCTTCAGAT+TGG	+	Chr7:90030510-90030529	MsG0780041360.01.T01:CDS	50.0%
	GAGCGATGGACGAGAGAAAA+AGG	+	Chr7:90030138-90030157	MsG0780041360.01.T01:CDS	50.0%
	GAGGAAGCAGAAACTGATGG+AGG	+	Chr7:90030205-90030224	MsG0780041360.01.T01:CDS	50.0%
	GGAGAGGAAGCAGAAACTGA+TGG	+	Chr7:90030202-90030221	MsG0780041360.01.T01:CDS	50.0%
	GGTTCAACGTGTGACGAGTT+CGG	+	Chr7:90030097-90030116	MsG0780041360.01.T01:CDS	50.0%
	GTTCAACGTGTGACGAGTTC+GGG	+	Chr7:90030098-90030117	MsG0780041360.01.T01:CDS	50.0%
	GTTGGAGAACATTAGGGCGA+GGG	+	Chr7:90030274-90030293	MsG0780041360.01.T01:CDS	50.0%
	TGTTGGAGAACATTAGGGCG+AGG	+	Chr7:90030273-90030292	MsG0780041360.01.T01:CDS	50.0%
	TTTCGAACCGTGAATCCGCA+CGG	+	Chr7:90030171-90030190	MsG0780041360.01.T01:CDS	50.0%
!	TTCGGACAAAGCATCTGCCA+CGG	-	Chr7:90030479-90030498	None:intergenic	50.0%
	AGCTCCGAGGACACCATTAG+CGG	-	Chr7:90030324-90030343	None:intergenic	55.0%
	ATCTGCCACGGGTTCAGTTG+CGG	-	Chr7:90030467-90030486	None:intergenic	55.0%
	ATTCCTGTATCCGAGCTCCG+AGG	-	Chr7:90030337-90030356	None:intergenic	55.0%
	CTAATGGTGTCCTCGGAGCT+CGG	+	Chr7:90030324-90030343	MsG0780041360.01.T01:CDS	55.0%
	GAAGCCGCTAATGGTGTCCT+CGG	+	Chr7:90030317-90030336	MsG0780041360.01.T01:CDS	55.0%
!	ACGTGTGACGAGTTCGGGTT+CGG	+	Chr7:90030103-90030122	MsG0780041360.01.T01:CDS	55.0%
!	TCGGACAAAGCATCTGCCAC+GGG	-	Chr7:90030478-90030497	None:intergenic	55.0%
	CACGGGTTCAGTTGCGGATC+TGG	-	Chr7:90030461-90030480	None:intergenic	60.0%
	CAGATCCGCAACTGAACCCG+TGG	+	Chr7:90030459-90030478	MsG0780041360.01.T01:CDS	60.0%
	GGACGGTGTGAATGGAGCGA+TGG	+	Chr7:90030124-90030143	MsG0780041360.01.T01:CDS	60.0%
	GTGAATCCGCACGGCGATCA+CGG	+	Chr7:90030180-90030199	MsG0780041360.01.T01:CDS	60.0%
	GTGATCGCCGTGCGGATTCA+CGG	-	Chr7:90030181-90030200	None:intergenic	60.0%
	TCGGGTTCGGACGGTGTGAA+TGG	+	Chr7:90030116-90030135	MsG0780041360.01.T01:CDS	60.0%
	TGAATCCGCACGGCGATCAC+GGG	+	Chr7:90030181-90030200	MsG0780041360.01.T01:CDS	60.0%
	TGTCCTCGGAGCTCGGATAC+AGG	+	Chr7:90030331-90030350	MsG0780041360.01.T01:CDS	60.0%
!	GTGACGAGTTCGGGTTCGGA+CGG	+	Chr7:90030107-90030126	MsG0780041360.01.T01:CDS	60.0%
	CCTCTCCCGTGATCGCCGTG+CGG	-	Chr7:90030189-90030208	None:intergenic	70.0%
	CCGCACGGCGATCACGGGAG+AGG	+	Chr7:90030186-90030205	MsG0780041360.01.T01:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
Chr7	gene	90030089	90030544	90030089	ID=MsG0780041360.01;Name=MsG0780041360.01
Chr7	mRNA	90030089	90030544	90030089	ID=MsG0780041360.01.T01;Parent=MsG0780041360.01;Name=MsG0780041360.01.T01;_AED=0.30;_eAED=0.30;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|151
Chr7	exon	90030089	90030544	90030089	ID=MsG0780041360.01.T01:exon:20746;Parent=MsG0780041360.01.T01
Chr7	CDS	90030089	90030544	90030089	ID=MsG0780041360.01.T01:cds;Parent=MsG0780041360.01.T01

Gene Sequence

>MsG0780041360.01.T01

ATGGCTTCGGTTCAACGTGTGACGAGTTCGGGTTCGGACGGTGTGAATGGAGCGATGGACGAGAGAAAAAGGAAGAGAATGATTTCGAACCGTGAATCCGCACGGCGATCACGGGAGAGGAAGCAGAAACTGATGGAGGATCTTCAAGATGAGACAAACAGGTTGCGAAACGAGAATCGGCGATTGTTGGAGAACATTAGGGCGAGGGAAGAAGGTTTCAATGCAAACGAAGCCGCTAATGGTGTCCTCGGAGCTCGGATACAGGAATTGACAGATCAATTGAAGTTTCTGAACTCGATCATTGAAAAAGCTGAGAGAGAAAAAGCTGAGAGGGAAAAGAATGGATTTTCTGTTGAGATTCCGAAGATTCCAGATCCGCAACTGAACCCGTGGCAGATGCTTTGTCCGAATCAAGCGATTAAGGCGTCTTCGTCTTCAGATTGGTTCTTGCATTGA

Protein sequence

>MsG0780041360.01.T01

MASVQRVTSSGSDGVNGAMDERKRKRMISNRESARRSRERKQKLMEDLQDETNRLRNENRRLLENIRAREEGFNANEAANGVLGARIQELTDQLKFLNSIIEKAEREKAEREKNGFSVEIPKIPDPQLNPWQMLCPNQAIKASSSSDWFLH*