Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041602.01.T01 | XP_003626191.1 | 95.604 | 91 | 4 | 0 | 28 | 118 | 35 | 125 | 1.36E-54 | 176 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041602.01.T01 | Q6NME7 | 53.086 | 81 | 37 | 1 | 29 | 108 | 36 | 116 | 2.00E-25 | 95.5 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047825.01 | MsG0780041602.01 | -0.804226 | 2.391111e-49 | 1.226113e-46 |
| MsG0180004116.01 | MsG0780041602.01 | -0.813585 | 2.376558e-51 | 1.558160e-48 |
| MsG0680035656.01 | MsG0780041602.01 | -0.805887 | 1.074211e-49 | 5.749189e-47 |
| MsG0480022597.01 | MsG0780041602.01 | -0.813502 | 2.477806e-51 | 1.620988e-48 |
| MsG0480022598.01 | MsG0780041602.01 | -0.802583 | 5.237857e-49 | 2.575183e-46 |
| MsG0580024853.01 | MsG0780041602.01 | -0.801842 | 7.439618e-49 | 3.589473e-46 |
| MsG0380015878.01 | MsG0780041602.01 | -0.817367 | 3.421290e-52 | 2.486702e-49 |
| MsG0780038829.01 | MsG0780041602.01 | -0.821991 | 3.007725e-53 | 2.485473e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041602.01.T01 | MTR_7g112530 | 95.604 | 91 | 4 | 0 | 28 | 118 | 35 | 125 | 1.64e-58 | 176 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041602.01.T01 | AT1G56260 | 53.086 | 81 | 37 | 1 | 29 | 108 | 36 | 116 | 2.04e-26 | 95.5 |
| MsG0780041602.01.T01 | AT1G56260 | 53.086 | 81 | 37 | 1 | 29 | 108 | 36 | 116 | 2.04e-26 | 95.5 |
Find 38 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGCATGTTTATATGCTTCT+AGG | 0.224256 | 7:-92915031 | MsG0780041602.01.T01:three_prime_UTR |
| TTTAGCAGTCTCTCTCTCTT+TGG | 0.287791 | 7:-92914751 | MsG0780041602.01.T01:three_prime_UTR |
| AGCCAACCATTTGAATAATT+CGG | 0.300995 | 7:-92915160 | MsG0780041602.01.T01:CDS |
| ATTCATTACATTGCACAATC+AGG | 0.318021 | 7:+92914784 | None:intergenic |
| ATTCGGCAACTTAACTTTGT+AGG | 0.318906 | 7:-92915143 | MsG0780041602.01.T01:exon |
| TTTACAAGTTACAACCTTCA+TGG | 0.334882 | 7:-92914959 | MsG0780041602.01.T01:three_prime_UTR |
| ATTATGATTGTGAAGTATCT+TGG | 0.365153 | 7:-92915631 | MsG0780041602.01.T01:CDS |
| GAGGCTCTGCGACAAACTTA+AGG | 0.393479 | 7:-92914811 | MsG0780041602.01.T01:three_prime_UTR |
| ATACTTCTATGAAAATAGTA+AGG | 0.422076 | 7:+92915081 | None:intergenic |
| GGATCTGTCTACCAATTCAT+TGG | 0.425515 | 7:-92915479 | MsG0780041602.01.T01:CDS |
| GACATTTAGTTGTAAATTTG+AGG | 0.431238 | 7:-92914830 | MsG0780041602.01.T01:three_prime_UTR |
| TAAGAAGCTCGCCAATGAAT+TGG | 0.437510 | 7:+92915468 | None:intergenic |
| AGCAGTCTCTCTCTCTTTGG+TGG | 0.440934 | 7:-92914748 | None:intergenic |
| CAGATCCAACTTGAAACTTA+AGG | 0.441876 | 7:+92915495 | None:intergenic |
| TTATGCTGTGCATGCTTCAC+GGG | 0.454326 | 7:-92914853 | MsG0780041602.01.T01:three_prime_UTR |
| TGCCGAATTATTCAAATGGT+TGG | 0.458705 | 7:+92915158 | None:intergenic |
| ATTATGCTGTGCATGCTTCA+CGG | 0.470976 | 7:-92914854 | MsG0780041602.01.T01:three_prime_UTR |
| CACGAATACTCTTTAGAGAC+AGG | 0.496480 | 7:-92915578 | MsG0780041602.01.T01:CDS |
| AAGGACCTTAAGTTTCAAGT+TGG | 0.504185 | 7:-92915500 | MsG0780041602.01.T01:CDS |
| TTCATTACATTGCACAATCA+GGG | 0.516627 | 7:+92914785 | None:intergenic |
| CACGTACCTCATCATCAGTT+CGG | 0.519854 | 7:+92915444 | None:intergenic |
| AAGTTGCCGAATTATTCAAA+TGG | 0.529169 | 7:+92915154 | None:intergenic |
| AAAATGAAATGTCACCATGA+AGG | 0.555013 | 7:+92914945 | None:intergenic |
| TTTATATGCTTCTAGGACAA+GGG | 0.567964 | 7:-92915024 | MsG0780041602.01.T01:three_prime_UTR |
| GTTTATATGCTTCTAGGACA+AGG | 0.581797 | 7:-92915025 | MsG0780041602.01.T01:three_prime_UTR |
| AAGAGTATTCGTGTAACCTG+TGG | 0.591562 | 7:+92915588 | None:intergenic |
| AGAGTATTCGTGTAACCTGT+GGG | 0.594484 | 7:+92915589 | None:intergenic |
| AGTTAGTACTGAGCATCTGA+AGG | 0.602957 | 7:-92915519 | MsG0780041602.01.T01:CDS |
| TACCATCGATGACTGTTGCA+AGG | 0.616671 | 7:+92915555 | None:intergenic |
| GGCCTTGCAACAGTCATCGA+TGG | 0.618120 | 7:-92915557 | MsG0780041602.01.T01:CDS |
| TACTTCTATGAAAATAGTAA+GGG | 0.622395 | 7:+92915082 | None:intergenic |
| AAACTGTTTCAAAAGCAGCA+AGG | 0.639053 | 7:+92915185 | None:intergenic |
| ACTTGTGTAATGCATCCCAC+AGG | 0.644354 | 7:-92915604 | MsG0780041602.01.T01:CDS |
| TCTTATCCGAACTGATGATG+AGG | 0.648922 | 7:-92915450 | MsG0780041602.01.T01:intron |
| CGTGTTGGTAGAAATGTCGA+CGG | 0.665620 | 7:-92915234 | MsG0780041602.01.T01:CDS |
| GTGTTGGTAGAAATGTCGAC+GGG | 0.670397 | 7:-92915233 | MsG0780041602.01.T01:CDS |
| GACATGATGACATCAAAGAG+TGG | 0.680973 | 7:-92914893 | MsG0780041602.01.T01:three_prime_UTR |
| TATGCTGTGCATGCTTCACG+GGG | 0.688025 | 7:-92914852 | MsG0780041602.01.T01:three_prime_UTR |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCTCAATATAAAAACATAAA+AGG | - | Chr7:92915305-92915324 | MsG0780041602.01.T01:intron | 15.0% |
| !! | ATACTTCTATGAAAATAGTA+AGG | + | Chr7:92915337-92915356 | None:intergenic | 20.0% |
| !! | TACTTCTATGAAAATAGTAA+GGG | + | Chr7:92915336-92915355 | None:intergenic | 20.0% |
| !!! | AATGTTCTTTGTTTTTTGTT+AGG | - | Chr7:92915144-92915163 | MsG0780041602.01.T01:exon | 20.0% |
| !!! | ATGTTCTTTGTTTTTTGTTA+GGG | - | Chr7:92915145-92915164 | MsG0780041602.01.T01:exon | 20.0% |
| !!! | TTTTATTATACGCTTTCTTT+TGG | - | Chr7:92915489-92915508 | MsG0780041602.01.T01:CDS | 20.0% |
| ! | GACATTTAGTTGTAAATTTG+AGG | - | Chr7:92915585-92915604 | MsG0780041602.01.T01:CDS | 25.0% |
| !! | ATTATGATTGTGAAGTATCT+TGG | - | Chr7:92914784-92914803 | MsG0780041602.01.T01:three_prime_UTR | 25.0% |
| AAAATGAAATGTCACCATGA+AGG | + | Chr7:92915473-92915492 | None:intergenic | 30.0% | |
| AAGCATGTTTATATGCTTCT+AGG | - | Chr7:92915384-92915403 | MsG0780041602.01.T01:intron | 30.0% | |
| AAGTTGCCGAATTATTCAAA+TGG | + | Chr7:92915264-92915283 | None:intergenic | 30.0% | |
| AGCCAACCATTTGAATAATT+CGG | - | Chr7:92915255-92915274 | MsG0780041602.01.T01:intron | 30.0% | |
| ATTCATTACATTGCACAATC+AGG | + | Chr7:92915634-92915653 | None:intergenic | 30.0% | |
| TTCATTACATTGCACAATCA+GGG | + | Chr7:92915633-92915652 | None:intergenic | 30.0% | |
| TTTACAAGTTACAACCTTCA+TGG | - | Chr7:92915456-92915475 | MsG0780041602.01.T01:CDS | 30.0% | |
| ! | TTTTCTATAGCAAACTCACT+TGG | - | Chr7:92915030-92915049 | MsG0780041602.01.T01:three_prime_UTR | 30.0% |
| !! | GTGAGTTTGCTATAGAAAAT+CGG | + | Chr7:92915029-92915048 | None:intergenic | 30.0% |
| !! | TGAGTTTGCTATAGAAAATC+GGG | + | Chr7:92915028-92915047 | None:intergenic | 30.0% |
| !! | TTTATATGCTTCTAGGACAA+GGG | - | Chr7:92915391-92915410 | MsG0780041602.01.T01:intron | 30.0% |
| AAACTGTTTCAAAAGCAGCA+AGG | + | Chr7:92915233-92915252 | None:intergenic | 35.0% | |
| ATTCGGCAACTTAACTTTGT+AGG | - | Chr7:92915272-92915291 | MsG0780041602.01.T01:intron | 35.0% | |
| CAGATCCAACTTGAAACTTA+AGG | + | Chr7:92914923-92914942 | None:intergenic | 35.0% | |
| TGCCGAATTATTCAAATGGT+TGG | + | Chr7:92915260-92915279 | None:intergenic | 35.0% | |
| ! | AAGGACCTTAAGTTTCAAGT+TGG | - | Chr7:92914915-92914934 | MsG0780041602.01.T01:three_prime_UTR | 35.0% |
| ! | TTTGCTATAGAAAATCGGGT+AGG | + | Chr7:92915024-92915043 | None:intergenic | 35.0% |
| ! | TTTTTGTTAGGGAGTTTTGC+AGG | - | Chr7:92915156-92915175 | MsG0780041602.01.T01:CDS | 35.0% |
| !! | GCTTTCTTTTGGCTACAAAT+AGG | - | Chr7:92915500-92915519 | MsG0780041602.01.T01:CDS | 35.0% |
| !! | GTTTATATGCTTCTAGGACA+AGG | - | Chr7:92915390-92915409 | MsG0780041602.01.T01:intron | 35.0% |
| ATAGAAAATCGGGTAGGCTA+AGG | + | Chr7:92915018-92915037 | None:intergenic | 40.0% | |
| ATTATGCTGTGCATGCTTCA+CGG | - | Chr7:92915561-92915580 | MsG0780041602.01.T01:CDS | 40.0% | |
| GACATGATGACATCAAAGAG+TGG | - | Chr7:92915522-92915541 | MsG0780041602.01.T01:CDS | 40.0% | |
| GGATCTGTCTACCAATTCAT+TGG | - | Chr7:92914936-92914955 | MsG0780041602.01.T01:three_prime_UTR | 40.0% | |
| TAAGAAGCTCGCCAATGAAT+TGG | + | Chr7:92914950-92914969 | None:intergenic | 40.0% | |
| TTTAGCAGTCTCTCTCTCTT+TGG | - | Chr7:92915664-92915683 | MsG0780041602.01.T01:CDS | 40.0% | |
| ! | AGAGTATTCGTGTAACCTGT+GGG | + | Chr7:92914829-92914848 | None:intergenic | 40.0% |
| ! | AGTTAGTACTGAGCATCTGA+AGG | - | Chr7:92914896-92914915 | MsG0780041602.01.T01:three_prime_UTR | 40.0% |
| ! | CACGAATACTCTTTAGAGAC+AGG | - | Chr7:92914837-92914856 | MsG0780041602.01.T01:three_prime_UTR | 40.0% |
| ! | TCTTATCCGAACTGATGATG+AGG | - | Chr7:92914965-92914984 | MsG0780041602.01.T01:three_prime_UTR | 40.0% |
| !! | AAGAGTATTCGTGTAACCTG+TGG | + | Chr7:92914830-92914849 | None:intergenic | 40.0% |
| !! | AGAGTGGCTTTTATTTGAGC+TGG | - | Chr7:92915538-92915557 | MsG0780041602.01.T01:CDS | 40.0% |
| AATACACGTTCACCCACAAC+CGG | - | Chr7:92915073-92915092 | MsG0780041602.01.T01:three_prime_UTR | 45.0% | |
| ACTTGTGTAATGCATCCCAC+AGG | - | Chr7:92914811-92914830 | MsG0780041602.01.T01:three_prime_UTR | 45.0% | |
| CACGTACCTCATCATCAGTT+CGG | + | Chr7:92914974-92914993 | None:intergenic | 45.0% | |
| TACCATCGATGACTGTTGCA+AGG | + | Chr7:92914863-92914882 | None:intergenic | 45.0% | |
| TTATGCTGTGCATGCTTCAC+GGG | - | Chr7:92915562-92915581 | MsG0780041602.01.T01:CDS | 45.0% | |
| ! | CGTGTTGGTAGAAATGTCGA+CGG | - | Chr7:92915181-92915200 | MsG0780041602.01.T01:CDS | 45.0% |
| ! | GTGTTGGTAGAAATGTCGAC+GGG | - | Chr7:92915182-92915201 | MsG0780041602.01.T01:CDS | 45.0% |
| GAGGCTCTGCGACAAACTTA+AGG | - | Chr7:92915604-92915623 | MsG0780041602.01.T01:CDS | 50.0% | |
| GATGAGGTACGTGCTTGTCA+AGG | - | Chr7:92914981-92915000 | MsG0780041602.01.T01:three_prime_UTR | 50.0% | |
| GGTACGTGCTTGTCAAGGTT+TGG | - | Chr7:92914986-92915005 | MsG0780041602.01.T01:three_prime_UTR | 50.0% | |
| TATGCTGTGCATGCTTCACG+GGG | - | Chr7:92915563-92915582 | MsG0780041602.01.T01:CDS | 50.0% | |
| TCACACCTTTCTGCATGCAC+CGG | + | Chr7:92915095-92915114 | None:intergenic | 50.0% | |
| !! | TTTCTGCATGCACCGGTTGT+GGG | + | Chr7:92915088-92915107 | None:intergenic | 50.0% |
| CACAACCGGTGCATGCAGAA+AGG | - | Chr7:92915087-92915106 | MsG0780041602.01.T01:three_prime_UTR | 55.0% | |
| GGCCTTGCAACAGTCATCGA+TGG | - | Chr7:92914858-92914877 | MsG0780041602.01.T01:three_prime_UTR | 55.0% | |
| ! | GGAGTTTTGCAGGCACGTGT+TGG | - | Chr7:92915166-92915185 | MsG0780041602.01.T01:CDS | 55.0% |
| !! | CTTTCTGCATGCACCGGTTG+TGG | + | Chr7:92915089-92915108 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 92914750 | 92915687 | 92914750 | ID=MsG0780041602.01;Name=MsG0780041602.01 |
| Chr7 | mRNA | 92914750 | 92915687 | 92914750 | ID=MsG0780041602.01.T01;Parent=MsG0780041602.01;Name=MsG0780041602.01.T01;_AED=0.45;_eAED=0.49;_QI=0|1|0.5|1|1|1|2|402|118 |
| Chr7 | exon | 92914750 | 92915271 | 92914750 | ID=MsG0780041602.01.T01:exon:40753;Parent=MsG0780041602.01.T01 |
| Chr7 | exon | 92915451 | 92915687 | 92915451 | ID=MsG0780041602.01.T01:exon:40752;Parent=MsG0780041602.01.T01 |
| Chr7 | CDS | 92915451 | 92915687 | 92915451 | ID=MsG0780041602.01.T01:cds;Parent=MsG0780041602.01.T01 |
| Chr7 | CDS | 92915152 | 92915271 | 92915152 | ID=MsG0780041602.01.T01:cds;Parent=MsG0780041602.01.T01 |
| Chr7 | three_prime_UTR | 92914750 | 92915151 | 92914750 | ID=MsG0780041602.01.T01:three_prime_utr;Parent=MsG0780041602.01.T01 |
| Gene Sequence |
| Protein sequence |
