AlfalfaGEDB | MsG0880042133.01

M. sativa cultivar ZhongmuNo.1 / MsG0880042133.01

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042133.01.T01	XP_003626951.1	92.381	105	7	1	1	105	1	104	1.75E-53	181

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042133.01.T01	G7LF69	92.381	105	7	1	1	105	1	104	8.36e-54	181

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180001149.01	MsG0880042133.01	0.812885	3.384304e-51	2.177287e-48
MsG0280010288.01	MsG0880042133.01	0.804996	1.651850e-49	8.640530e-47
MsG0380013342.01	MsG0880042133.01	0.815308	9.880555e-52	6.787795e-49
MsG0380014240.01	MsG0880042133.01	0.800527	1.382249e-48	6.450547e-46
MsG0480019408.01	MsG0880042133.01	0.802452	5.573432e-49	2.731140e-46
MsG0680030636.01	MsG0880042133.01	0.803847	2.866784e-49	1.455935e-46
MsG0680031334.01	MsG0880042133.01	0.803864	2.844022e-49	1.444990e-46
MsG0780036978.01	MsG0880042133.01	0.819231	1.294518e-52	9.904352e-50
MsG0780037330.01	MsG0880042133.01	0.803716	3.052523e-49	1.544913e-46
MsG0880042133.01	MsG0880042134.01	0.838582	2.639902e-57	3.551617e-54

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042133.01.T01	MTR_8g012420	92.381	105	7	1	1	105	1	104	2.12e-57	181

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042133.01.T01	AT3G07310	54.054	74	33	1	33	105	42	115	3.21e-17	75.9
MsG0880042133.01.T01	AT5G48590	44.706	85	40	1	21	105	34	111	2.57e-13	65.1

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Find 14 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACTTTCCAACTTGTGTACG+TGG	0.632779	8:-4183092	None:intergenic
ACTTGTGTACGTGGTAACAA+AGG	0.646831	8:-4183083	None:intergenic
CAAGTTGGAAAGTTTCTCAG+TGG	0.680223	8:+4183101	MsG0880042133.01.T01:CDS
CCAGTACTAGCCTTTATCAA+GGG	0.625800	8:-4183023	None:intergenic
CCCTTGATAAAGGCTAGTAC+TGG	0.509125	8:+4183023	MsG0880042133.01.T01:CDS
CTTACAATCAAACCTCACGT+AGG	0.718664	8:+4182942	MsG0880042133.01.T01:CDS
CTTGAACAGCAACATGAAAG+AGG	0.482807	8:-4183145	None:intergenic
CTTGTGAAGAATCCTACGTG+AGG	0.698404	8:-4182954	None:intergenic
GAGCAGAGTACCCTTGATAA+AGG	0.442386	8:+4183013	MsG0880042133.01.T01:CDS
GCAAGCTTCATCTGATTCAG+AGG	0.602873	8:-4183225	None:intergenic

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CRISPR-GE

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badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TCAAGAAGAGCTTAAACTAT+TGG	+	Chr8:4183163-4183182	MsG0880042133.01.T01:CDS	30.0%
	AACTTTCCAACTTGTGTACG+TGG	-	Chr8:4183095-4183114	None:intergenic	40.0%
	ACTTGTGTACGTGGTAACAA+AGG	-	Chr8:4183086-4183105	None:intergenic	40.0%
	CTTACAATCAAACCTCACGT+AGG	+	Chr8:4182942-4182961	MsG0880042133.01.T01:CDS	40.0%
	CTTGAACAGCAACATGAAAG+AGG	-	Chr8:4183148-4183167	None:intergenic	40.0%
	TTGTTACCACGTACACAAGT+TGG	+	Chr8:4183086-4183105	MsG0880042133.01.T01:CDS	40.0%
	CCCTTGATAAAGGCTAGTAC+TGG	+	Chr8:4183023-4183042	MsG0880042133.01.T01:CDS	45.0%
	CTTGTGAAGAATCCTACGTG+AGG	-	Chr8:4182957-4182976	None:intergenic	45.0%
	GCAAGCTTCATCTGATTCAG+AGG	-	Chr8:4183228-4183247	None:intergenic	45.0%
!	GAAGAAGATGAAGAAAAAAT+AGG	-	Chr8:4182981-4183000	None:intergenic	25.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	4182930	4183277	4182930	ID=MsG0880042133.01;Name=MsG0880042133.01
Chr8	mRNA	4182930	4183277	4182930	ID=MsG0880042133.01.T01;Parent=MsG0880042133.01;Name=MsG0880042133.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|115
Chr8	exon	4182930	4183277	4182930	ID=MsG0880042133.01.T01:exon:1706;Parent=MsG0880042133.01.T01
Chr8	CDS	4182930	4183277	4182930	ID=MsG0880042133.01.T01:cds;Parent=MsG0880042133.01.T01

Gene Sequence	>MsG0880042133.01.T01 ATGGAAAATTGTCTTACAATCAAACCTCACGTAGGATTCTTCACAAGACCTATTTTTTCTTCATCTTCTTCTTTGAATTTTAGGAGCAGAGTACCCTTGATAAAGGCTAGTACTGGAGCTAGTAGTCACTGTGAGTCAAGTAGTTTGAACACACCTTTGTTACCACGTACACAAGTTGGAAAGTTTCTCAGTGGTGTGTTGCAGAATCATCGTAACCTCTTTCATGTTGCTGTTCAAGAAGAGCTTAAACTATTGGCTGATGATAGAGATGCTGCTAATTCTCGTATGCTTCTTGCCTCTGAATCAGATGAAGCTTGCTTCACAGGTTTGTGTCCATGCCCTTTTTAG
Protein sequence	>MsG0880042133.01.T01 MENCLTIKPHVGFFTRPIFSSSSSLNFRSRVPLIKASTGASSHCESSSLNTPLLPRTQVGKFLSGVLQNHRNLFHVAVQEELKLLADDRDAANSRMLLASESDEACFTGLCPCPF*