Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042133.01.T01 | XP_003626951.1 | 92.381 | 105 | 7 | 1 | 1 | 105 | 1 | 104 | 1.75E-53 | 181 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042133.01.T01 | G7LF69 | 92.381 | 105 | 7 | 1 | 1 | 105 | 1 | 104 | 8.36e-54 | 181 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180001149.01 | MsG0880042133.01 | 0.812885 | 3.384304e-51 | 2.177287e-48 |
| MsG0880042133.01 | MsG0880042134.01 | 0.838582 | 2.639902e-57 | 3.551617e-54 |
| MsG0880042133.01 | MsG0880043867.01 | 0.800864 | 1.180119e-48 | 5.554751e-46 |
| MsG0380013342.01 | MsG0880042133.01 | 0.815308 | 9.880555e-52 | 6.787795e-49 |
| MsG0380014240.01 | MsG0880042133.01 | 0.800527 | 1.382249e-48 | 6.450547e-46 |
| MsG0680031334.01 | MsG0880042133.01 | 0.803864 | 2.844022e-49 | 1.444990e-46 |
| MsG0480019408.01 | MsG0880042133.01 | 0.802452 | 5.573432e-49 | 2.731140e-46 |
| MsG0680030636.01 | MsG0880042133.01 | 0.803847 | 2.866784e-49 | 1.455935e-46 |
| MsG0280010288.01 | MsG0880042133.01 | 0.804996 | 1.651850e-49 | 8.640530e-47 |
| MsG0780036978.01 | MsG0880042133.01 | 0.819231 | 1.294518e-52 | 9.904352e-50 |
| MsG0780037330.01 | MsG0880042133.01 | 0.803716 | 3.052523e-49 | 1.544913e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042133.01.T01 | MTR_8g012420 | 92.381 | 105 | 7 | 1 | 1 | 105 | 1 | 104 | 2.12e-57 | 181 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042133.01.T01 | AT3G07310 | 54.054 | 74 | 33 | 1 | 33 | 105 | 42 | 115 | 3.21e-17 | 75.9 |
| MsG0880042133.01.T01 | AT5G48590 | 44.706 | 85 | 40 | 1 | 21 | 105 | 34 | 111 | 2.57e-13 | 65.1 |
Find 14 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAAGAAGAGCTTAAACTAT+TGG | 0.349395 | 8:+4183163 | MsG0880042133.01.T01:CDS |
| TCAGATGAAGCTTGCTTCAC+AGG | 0.404400 | 8:+4183233 | MsG0880042133.01.T01:CDS |
| GAGCAGAGTACCCTTGATAA+AGG | 0.442386 | 8:+4183013 | MsG0880042133.01.T01:CDS |
| TCCAGTACTAGCCTTTATCA+AGG | 0.471270 | 8:-4183024 | None:intergenic |
| CTTGAACAGCAACATGAAAG+AGG | 0.482807 | 8:-4183145 | None:intergenic |
| CCCTTGATAAAGGCTAGTAC+TGG | 0.509125 | 8:+4183023 | MsG0880042133.01.T01:CDS |
| TTGTTACCACGTACACAAGT+TGG | 0.516122 | 8:+4183086 | MsG0880042133.01.T01:CDS |
| GCAAGCTTCATCTGATTCAG+AGG | 0.602873 | 8:-4183225 | None:intergenic |
| CCAGTACTAGCCTTTATCAA+GGG | 0.625800 | 8:-4183023 | None:intergenic |
| AACTTTCCAACTTGTGTACG+TGG | 0.632779 | 8:-4183092 | None:intergenic |
| ACTTGTGTACGTGGTAACAA+AGG | 0.646831 | 8:-4183083 | None:intergenic |
| CAAGTTGGAAAGTTTCTCAG+TGG | 0.680223 | 8:+4183101 | MsG0880042133.01.T01:CDS |
| CTTGTGAAGAATCCTACGTG+AGG | 0.698404 | 8:-4182954 | None:intergenic |
| CTTACAATCAAACCTCACGT+AGG | 0.718664 | 8:+4182942 | MsG0880042133.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GAAGAAGATGAAGAAAAAAT+AGG | - | Chr8:4182981-4183000 | None:intergenic | 25.0% |
| !! | CATCTTCTTCTTTGAATTTT+AGG | + | Chr8:4182991-4183010 | MsG0880042133.01.T01:CDS | 25.0% |
| TCAAGAAGAGCTTAAACTAT+TGG | + | Chr8:4183163-4183182 | MsG0880042133.01.T01:CDS | 30.0% | |
| AACTTTCCAACTTGTGTACG+TGG | - | Chr8:4183095-4183114 | None:intergenic | 40.0% | |
| ACTTGTGTACGTGGTAACAA+AGG | - | Chr8:4183086-4183105 | None:intergenic | 40.0% | |
| CTTACAATCAAACCTCACGT+AGG | + | Chr8:4182942-4182961 | MsG0880042133.01.T01:CDS | 40.0% | |
| CTTGAACAGCAACATGAAAG+AGG | - | Chr8:4183148-4183167 | None:intergenic | 40.0% | |
| TTGTTACCACGTACACAAGT+TGG | + | Chr8:4183086-4183105 | MsG0880042133.01.T01:CDS | 40.0% | |
| ! | CAAGTTGGAAAGTTTCTCAG+TGG | + | Chr8:4183101-4183120 | MsG0880042133.01.T01:CDS | 40.0% |
| !! | CCAGTACTAGCCTTTATCAA+GGG | - | Chr8:4183026-4183045 | None:intergenic | 40.0% |
| !! | TCCAGTACTAGCCTTTATCA+AGG | - | Chr8:4183027-4183046 | None:intergenic | 40.0% |
| CCCTTGATAAAGGCTAGTAC+TGG | + | Chr8:4183023-4183042 | MsG0880042133.01.T01:CDS | 45.0% | |
| CTTGTGAAGAATCCTACGTG+AGG | - | Chr8:4182957-4182976 | None:intergenic | 45.0% | |
| GCAAGCTTCATCTGATTCAG+AGG | - | Chr8:4183228-4183247 | None:intergenic | 45.0% | |
| ! | GAGCAGAGTACCCTTGATAA+AGG | + | Chr8:4183013-4183032 | MsG0880042133.01.T01:CDS | 45.0% |
| ! | TCAGATGAAGCTTGCTTCAC+AGG | + | Chr8:4183233-4183252 | MsG0880042133.01.T01:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 4182930 | 4183277 | 4182930 | ID=MsG0880042133.01;Name=MsG0880042133.01 |
| Chr8 | mRNA | 4182930 | 4183277 | 4182930 | ID=MsG0880042133.01.T01;Parent=MsG0880042133.01;Name=MsG0880042133.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|115 |
| Chr8 | exon | 4182930 | 4183277 | 4182930 | ID=MsG0880042133.01.T01:exon:1706;Parent=MsG0880042133.01.T01 |
| Chr8 | CDS | 4182930 | 4183277 | 4182930 | ID=MsG0880042133.01.T01:cds;Parent=MsG0880042133.01.T01 |
| Gene Sequence |
| Protein sequence |
