AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880042134.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880042134.01.T01 MTR_8g012420 100.000 83 0 0 1 83 253 335 1.45e-56 178
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 20 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTAAAGAGGCTGTGAATT+TGG 0.233214 8:+4185156 MsG0880042134.01.T01:CDS
GGGTTCGAGGACGACATCTT+TGG 0.387264 8:+4185010 MsG0880042134.01.T01:CDS
GTTAAAGATATGTGTCCATA+TGG 0.387690 8:+4184989 None:intergenic
ACTTGAAGTGTTATGTTATG+AGG 0.433392 8:+4185095 MsG0880042134.01.T01:CDS
CTTATTAGCCCTTGCCCGAT+CGG 0.435733 8:-4185052 None:intergenic
GCACACCTCTGCAATGATCC+AGG 0.438675 8:-4185124 None:intergenic
AAGAGGCTGTGAATTTGGTT+AGG 0.449980 8:+4185161 MsG0880042134.01.T01:CDS
ATGTCGTCCTCGAACCCATA+TGG 0.461362 8:-4185004 None:intergenic
AACATGAAACCGATCGGGCA+AGG 0.516793 8:+4185043 MsG0880042134.01.T01:CDS
CGGGCAAGGGCTAATAAGAC+AGG 0.537440 8:+4185057 MsG0880042134.01.T01:CDS
TGCTAAATTGAGATCTAAAG+AGG 0.547974 8:+4185144 MsG0880042134.01.T01:CDS
AGGGCTAATAAGACAGGAAG+AGG 0.551019 8:+4185063 MsG0880042134.01.T01:CDS
TGAGTAACATGAAACCGATC+GGG 0.557241 8:+4185038 MsG0880042134.01.T01:CDS
TTAAAGATATGTGTCCATAT+GGG 0.570422 8:+4184990 None:intergenic
ACATGAAACCGATCGGGCAA+GGG 0.575846 8:+4185044 MsG0880042134.01.T01:CDS
TTGAGTAACATGAAACCGAT+CGG 0.579919 8:+4185037 MsG0880042134.01.T01:CDS
TATGTGTCCATATGGGTTCG+AGG 0.583345 8:+4184997 None:intergenic
TTCACCCTGGATCATTGCAG+AGG 0.619675 8:+4185119 MsG0880042134.01.T01:CDS
CACACCTCTGCAATGATCCA+GGG 0.620978 8:-4185123 None:intergenic
TATGTTATGAGGTTTCACCC+TGG 0.650313 8:+4185106 MsG0880042134.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TGCTAAATTGAGATCTAAAG+AGG + Chr8:4185144-4185163 MsG0880042134.01.T01:CDS 30.0%
! ACTTGAAGTGTTATGTTATG+AGG + Chr8:4185095-4185114 MsG0880042134.01.T01:CDS 30.0%
ATCTAAAGAGGCTGTGAATT+TGG + Chr8:4185156-4185175 MsG0880042134.01.T01:CDS 35.0%
TTGAGTAACATGAAACCGAT+CGG + Chr8:4185037-4185056 MsG0880042134.01.T01:CDS 35.0%
! CTGCTCTTTTTAACAGTGAA+GGG + Chr8:4185194-4185213 MsG0880042134.01.T01:CDS 35.0%
! TCTGCTCTTTTTAACAGTGA+AGG + Chr8:4185193-4185212 MsG0880042134.01.T01:CDS 35.0%
AAGAGGCTGTGAATTTGGTT+AGG + Chr8:4185161-4185180 MsG0880042134.01.T01:CDS 40.0%
TATGTTATGAGGTTTCACCC+TGG + Chr8:4185106-4185125 MsG0880042134.01.T01:CDS 40.0%
TGAGTAACATGAAACCGATC+GGG + Chr8:4185038-4185057 MsG0880042134.01.T01:CDS 40.0%
AGGGCTAATAAGACAGGAAG+AGG + Chr8:4185063-4185082 MsG0880042134.01.T01:CDS 45.0%
AACATGAAACCGATCGGGCA+AGG + Chr8:4185043-4185062 MsG0880042134.01.T01:CDS 50.0%
ACATGAAACCGATCGGGCAA+GGG + Chr8:4185044-4185063 MsG0880042134.01.T01:CDS 50.0%
ATGTCGTCCTCGAACCCATA+TGG - Chr8:4185007-4185026 None:intergenic 50.0%
CACACCTCTGCAATGATCCA+GGG - Chr8:4185126-4185145 None:intergenic 50.0%
CTTATTAGCCCTTGCCCGAT+CGG - Chr8:4185055-4185074 None:intergenic 50.0%
TTCACCCTGGATCATTGCAG+AGG + Chr8:4185119-4185138 MsG0880042134.01.T01:CDS 50.0%
CGGGCAAGGGCTAATAAGAC+AGG + Chr8:4185057-4185076 MsG0880042134.01.T01:CDS 55.0%
GCACACCTCTGCAATGATCC+AGG - Chr8:4185127-4185146 None:intergenic 55.0%
GGGTTCGAGGACGACATCTT+TGG + Chr8:4185010-4185029 MsG0880042134.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 4184998 4185249 4184998 ID=MsG0880042134.01;Name=MsG0880042134.01
Chr8 mRNA 4184998 4185249 4184998 ID=MsG0880042134.01.T01;Parent=MsG0880042134.01;Name=MsG0880042134.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|83
Chr8 exon 4184998 4185249 4184998 ID=MsG0880042134.01.T01:exon:1707;Parent=MsG0880042134.01.T01
Chr8 CDS 4184998 4185249 4184998 ID=MsG0880042134.01.T01:cds;Parent=MsG0880042134.01.T01
Gene Sequence

>MsG0880042134.01.T01

ATGTGTCCATATGGGTTCGAGGACGACATCTTTGGTCATTTGAGTAACATGAAACCGATCGGGCAAGGGCTAATAAGACAGGAAGAGGAAATTGAAGACTTGAAGTGTTATGTTATGAGGTTTCACCCTGGATCATTGCAGAGGTGTGCTAAATTGAGATCTAAAGAGGCTGTGAATTTGGTTAGGAGTTATAGTTCTGCTCTTTTTAACAGTGAAGGGTTTGATTCAGTTGACAGTGATGATGTTATTTGA

Protein sequence

>MsG0880042134.01.T01

MCPYGFEDDIFGHLSNMKPIGQGLIRQEEEIEDLKCYVMRFHPGSLQRCAKLRSKEAVNLVRSYSSALFNSEGFDSVDSDDVI*