Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042688.01.T01 | XP_039684615.1 | 99.148 | 352 | 3 | 0 | 1 | 352 | 1 | 352 | 0 | 717 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042688.01.T01 | Q54VX4 | 29.722 | 360 | 209 | 13 | 10 | 346 | 7 | 345 | 2.47E-38 | 142 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042688.01.T01 | A0A072TMM7 | 99.148 | 352 | 3 | 0 | 1 | 352 | 1 | 352 | 0.0 | 717 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180005361.01 | MsG0880042688.01 | 0.823744 | 1.174367e-53 | 1.020090e-50 |
| MsG0880042688.01 | MsG0880045166.01 | 0.825086 | 5.677144e-54 | 5.123401e-51 |
| MsG0480023360.01 | MsG0880042688.01 | 0.841086 | 5.879232e-58 | 8.543356e-55 |
| MsG0280006700.01 | MsG0880042688.01 | 0.811869 | 5.642264e-51 | 3.532569e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042688.01.T01 | MTR_8g019480 | 99.148 | 352 | 3 | 0 | 1 | 352 | 1 | 352 | 0.0 | 717 |
| MsG0880042688.01.T01 | MTR_3g104810 | 64.407 | 354 | 115 | 4 | 1 | 349 | 1 | 348 | 8.89e-157 | 444 |
| MsG0880042688.01.T01 | MTR_6g015860 | 44.776 | 335 | 155 | 6 | 21 | 351 | 5 | 313 | 4.23e-93 | 281 |
| MsG0880042688.01.T01 | MTR_7g015870 | 45.732 | 328 | 150 | 4 | 21 | 345 | 9 | 311 | 5.27e-93 | 281 |
| MsG0880042688.01.T01 | MTR_7g015870 | 46.154 | 325 | 147 | 4 | 27 | 348 | 19 | 318 | 7.06e-93 | 280 |
| MsG0880042688.01.T01 | MTR_2g032610 | 40.122 | 329 | 168 | 6 | 21 | 344 | 17 | 321 | 3.48e-81 | 250 |
| MsG0880042688.01.T01 | MTR_1g097890 | 27.124 | 306 | 165 | 7 | 43 | 348 | 61 | 308 | 2.67e-26 | 107 |
| MsG0880042688.01.T01 | MTR_8g092080 | 27.124 | 306 | 165 | 8 | 43 | 348 | 62 | 309 | 8.21e-22 | 94.7 |
| MsG0880042688.01.T01 | MTR_8g092080 | 26.500 | 200 | 117 | 6 | 30 | 221 | 128 | 305 | 8.79e-11 | 62.8 |
| MsG0880042688.01.T01 | MTR_5g073040 | 27.667 | 300 | 165 | 7 | 47 | 338 | 53 | 308 | 3.78e-21 | 93.2 |
| MsG0880042688.01.T01 | MTR_5g073040 | 31.443 | 194 | 100 | 6 | 41 | 218 | 135 | 311 | 1.55e-12 | 68.2 |
| MsG0880042688.01.T01 | MTR_4g114320 | 24.921 | 317 | 195 | 8 | 34 | 348 | 123 | 398 | 7.37e-21 | 93.2 |
| MsG0880042688.01.T01 | MTR_4g012850 | 23.642 | 313 | 202 | 7 | 40 | 350 | 21 | 298 | 1.19e-16 | 79.7 |
| MsG0880042688.01.T01 | MTR_8g098240 | 26.101 | 318 | 185 | 10 | 27 | 341 | 314 | 584 | 1.69e-16 | 80.9 |
| MsG0880042688.01.T01 | MTR_1g012030 | 24.324 | 296 | 182 | 8 | 49 | 343 | 155 | 409 | 4.89e-16 | 79.3 |
| MsG0880042688.01.T01 | MTR_1g012030 | 26.047 | 215 | 119 | 4 | 132 | 346 | 140 | 314 | 3.83e-12 | 67.4 |
| MsG0880042688.01.T01 | MTR_4g088545 | 24.606 | 317 | 198 | 11 | 37 | 343 | 14 | 299 | 1.06e-15 | 77.0 |
| MsG0880042688.01.T01 | MTR_7g068340 | 27.442 | 215 | 130 | 6 | 35 | 247 | 187 | 377 | 7.36e-15 | 75.5 |
| MsG0880042688.01.T01 | MTR_7g064980 | 24.828 | 290 | 179 | 7 | 62 | 348 | 65 | 318 | 1.81e-14 | 73.6 |
| MsG0880042688.01.T01 | MTR_7g109135 | 23.102 | 303 | 190 | 8 | 49 | 343 | 38 | 305 | 4.06e-14 | 72.8 |
| MsG0880042688.01.T01 | MTR_2g102010 | 25.818 | 275 | 166 | 7 | 48 | 320 | 32 | 270 | 1.82e-13 | 70.5 |
| MsG0880042688.01.T01 | MTR_8g027975 | 30.846 | 201 | 124 | 7 | 35 | 221 | 154 | 353 | 2.93e-13 | 70.5 |
| MsG0880042688.01.T01 | MTR_8g027975 | 23.571 | 280 | 175 | 6 | 77 | 343 | 99 | 352 | 2.15e-11 | 64.7 |
| MsG0880042688.01.T01 | MTR_4g016730 | 28.272 | 191 | 102 | 5 | 35 | 221 | 196 | 355 | 4.63e-13 | 70.1 |
| MsG0880042688.01.T01 | MTR_8g076760 | 25.080 | 311 | 196 | 11 | 47 | 347 | 28 | 311 | 5.53e-13 | 69.3 |
| MsG0880042688.01.T01 | MTR_5g055410 | 29.612 | 206 | 113 | 8 | 49 | 239 | 135 | 323 | 2.11e-12 | 67.8 |
| MsG0880042688.01.T01 | MTR_5g055410 | 25.828 | 302 | 183 | 9 | 49 | 343 | 37 | 304 | 3.29e-11 | 64.3 |
| MsG0880042688.01.T01 | MTR_5g009500 | 29.500 | 200 | 126 | 7 | 35 | 221 | 141 | 338 | 2.64e-12 | 67.4 |
| MsG0880042688.01.T01 | MTR_5g009500 | 24.820 | 278 | 172 | 6 | 75 | 341 | 84 | 335 | 9.58e-11 | 62.8 |
| MsG0880042688.01.T01 | MTR_2g098070 | 31.579 | 152 | 84 | 4 | 74 | 223 | 221 | 354 | 7.55e-12 | 66.2 |
| MsG0880042688.01.T01 | MTR_3g107510 | 26.941 | 219 | 112 | 5 | 124 | 342 | 123 | 293 | 8.80e-12 | 66.2 |
| MsG0880042688.01.T01 | MTR_4g119770 | 29.319 | 191 | 113 | 8 | 40 | 214 | 134 | 318 | 2.72e-11 | 64.3 |
| MsG0880042688.01.T01 | MTR_1g087840 | 24.437 | 311 | 176 | 10 | 49 | 343 | 35 | 302 | 3.02e-11 | 63.9 |
| MsG0880042688.01.T01 | MTR_2g436930 | 28.191 | 188 | 121 | 7 | 41 | 216 | 44 | 229 | 3.32e-11 | 63.2 |
| MsG0880042688.01.T01 | MTR_2g436930 | 23.571 | 280 | 169 | 9 | 73 | 339 | 72 | 319 | 4.72e-11 | 63.5 |
| MsG0880042688.01.T01 | MTR_2g436930 | 28.191 | 188 | 121 | 7 | 41 | 216 | 134 | 319 | 6.04e-11 | 63.2 |
| MsG0880042688.01.T01 | MTR_6g034515 | 30.857 | 175 | 114 | 5 | 44 | 218 | 171 | 338 | 6.74e-11 | 63.2 |
| MsG0880042688.01.T01 | MTR_7g084400 | 28.796 | 191 | 115 | 5 | 34 | 219 | 309 | 483 | 7.58e-11 | 63.5 |
| MsG0880042688.01.T01 | MTR_4g057850 | 24.490 | 294 | 162 | 10 | 44 | 322 | 33 | 281 | 7.91e-11 | 62.8 |
| MsG0880042688.01.T01 | MTR_8g096050 | 26.977 | 215 | 120 | 4 | 132 | 346 | 228 | 405 | 8.66e-11 | 63.5 |
| MsG0880042688.01.T01 | MTR_1g095780 | 24.383 | 324 | 186 | 9 | 35 | 352 | 522 | 792 | 9.71e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042688.01.T01 | AT1G72820 | 73.011 | 352 | 83 | 5 | 1 | 349 | 1 | 343 | 1.66e-180 | 504 |
| MsG0880042688.01.T01 | AT1G72820 | 73.011 | 352 | 83 | 5 | 1 | 349 | 1 | 343 | 1.66e-180 | 504 |
| MsG0880042688.01.T01 | AT5G26200 | 61.947 | 339 | 108 | 3 | 15 | 349 | 21 | 342 | 2.11e-148 | 422 |
| MsG0880042688.01.T01 | AT5G15640 | 47.416 | 329 | 145 | 6 | 21 | 345 | 16 | 320 | 3.97e-95 | 286 |
| MsG0880042688.01.T01 | AT5G15640 | 47.256 | 328 | 144 | 7 | 22 | 345 | 11 | 313 | 2.34e-92 | 279 |
| MsG0880042688.01.T01 | AT1G34065 | 26.623 | 308 | 167 | 9 | 43 | 349 | 85 | 334 | 7.82e-23 | 97.8 |
| MsG0880042688.01.T01 | AT1G34065 | 26.623 | 308 | 167 | 9 | 43 | 349 | 92 | 341 | 8.93e-23 | 97.8 |
| MsG0880042688.01.T01 | AT4G11440 | 26.667 | 315 | 189 | 8 | 29 | 341 | 326 | 600 | 9.09e-22 | 97.1 |
| MsG0880042688.01.T01 | AT4G11440 | 26.667 | 315 | 189 | 8 | 29 | 341 | 326 | 600 | 9.09e-22 | 97.1 |
| MsG0880042688.01.T01 | AT4G39460 | 25.817 | 306 | 169 | 9 | 43 | 348 | 68 | 315 | 3.88e-19 | 87.4 |
| MsG0880042688.01.T01 | AT4G39460 | 25.817 | 306 | 169 | 9 | 43 | 348 | 68 | 315 | 3.88e-19 | 87.4 |
| MsG0880042688.01.T01 | AT4G39460 | 25.817 | 306 | 169 | 9 | 43 | 348 | 68 | 315 | 3.88e-19 | 87.4 |
| MsG0880042688.01.T01 | AT2G37890 | 26.502 | 283 | 162 | 8 | 68 | 343 | 88 | 331 | 5.51e-19 | 87.0 |
| MsG0880042688.01.T01 | AT1G07030 | 27.273 | 319 | 185 | 7 | 31 | 343 | 38 | 315 | 9.50e-19 | 86.3 |
| MsG0880042688.01.T01 | AT2G30160 | 27.815 | 302 | 176 | 9 | 47 | 343 | 56 | 320 | 2.64e-18 | 85.1 |
| MsG0880042688.01.T01 | AT2G30160 | 31.088 | 193 | 114 | 5 | 37 | 218 | 134 | 318 | 4.99e-13 | 69.7 |
| MsG0880042688.01.T01 | AT2G30160 | 26.066 | 211 | 116 | 5 | 132 | 342 | 43 | 213 | 1.70e-12 | 68.2 |
| MsG0880042688.01.T01 | AT3G53940 | 26.354 | 277 | 160 | 7 | 73 | 343 | 121 | 359 | 8.48e-16 | 78.2 |
| MsG0880042688.01.T01 | AT4G32400 | 23.585 | 318 | 200 | 9 | 34 | 349 | 114 | 390 | 6.87e-15 | 75.5 |
| MsG0880042688.01.T01 | AT3G20240 | 25.581 | 301 | 173 | 8 | 34 | 320 | 52 | 315 | 2.34e-14 | 73.6 |
| MsG0880042688.01.T01 | AT3G20240 | 29.444 | 180 | 105 | 6 | 41 | 214 | 169 | 332 | 6.81e-13 | 69.3 |
| MsG0880042688.01.T01 | AT1G79900 | 24.746 | 295 | 178 | 8 | 48 | 342 | 31 | 281 | 4.89e-14 | 72.4 |
| MsG0880042688.01.T01 | AT1G79900 | 29.050 | 179 | 112 | 3 | 44 | 222 | 121 | 284 | 6.94e-12 | 65.9 |
| MsG0880042688.01.T01 | AT5G01340 | 28.041 | 296 | 150 | 12 | 44 | 322 | 28 | 277 | 4.35e-13 | 69.7 |
| MsG0880042688.01.T01 | AT5G66380 | 24.430 | 307 | 196 | 9 | 45 | 343 | 22 | 300 | 5.99e-13 | 69.3 |
| MsG0880042688.01.T01 | AT3G51870 | 25.592 | 211 | 130 | 4 | 35 | 243 | 191 | 376 | 9.54e-13 | 68.9 |
| MsG0880042688.01.T01 | AT1G25380 | 24.919 | 309 | 178 | 9 | 49 | 343 | 37 | 305 | 2.95e-12 | 67.4 |
| MsG0880042688.01.T01 | AT1G25380 | 24.919 | 309 | 178 | 9 | 49 | 343 | 37 | 305 | 2.95e-12 | 67.4 |
| MsG0880042688.01.T01 | AT5G48970 | 23.496 | 349 | 208 | 10 | 19 | 339 | 12 | 329 | 3.91e-12 | 67.0 |
| MsG0880042688.01.T01 | AT1G74240 | 24.922 | 321 | 181 | 13 | 29 | 322 | 27 | 314 | 6.77e-12 | 66.6 |
| MsG0880042688.01.T01 | AT2G47490 | 25.000 | 308 | 177 | 11 | 49 | 343 | 33 | 299 | 6.95e-12 | 65.9 |
| MsG0880042688.01.T01 | AT5G51050 | 23.438 | 320 | 202 | 9 | 25 | 342 | 215 | 493 | 1.24e-11 | 66.2 |
| MsG0880042688.01.T01 | AT5G48970 | 24.188 | 277 | 174 | 7 | 73 | 339 | 8 | 258 | 1.44e-11 | 64.7 |
| MsG0880042688.01.T01 | AT5G48970 | 27.193 | 228 | 120 | 9 | 11 | 214 | 51 | 256 | 4.02e-11 | 63.2 |
| MsG0880042688.01.T01 | AT4G01100 | 28.436 | 211 | 134 | 8 | 35 | 231 | 95 | 302 | 2.39e-11 | 64.3 |
| MsG0880042688.01.T01 | AT1G74240 | 25.503 | 298 | 167 | 12 | 47 | 322 | 2 | 266 | 2.56e-11 | 64.3 |
| MsG0880042688.01.T01 | AT3G54110 | 25.574 | 305 | 176 | 10 | 49 | 342 | 31 | 295 | 2.98e-11 | 63.9 |
| MsG0880042688.01.T01 | AT4G01100 | 28.436 | 211 | 134 | 8 | 35 | 231 | 145 | 352 | 3.92e-11 | 63.9 |
| MsG0880042688.01.T01 | AT5G46800 | 26.606 | 218 | 122 | 7 | 132 | 341 | 109 | 296 | 5.07e-11 | 63.2 |
| MsG0880042688.01.T01 | AT5G46800 | 26.606 | 218 | 122 | 7 | 132 | 341 | 109 | 296 | 5.07e-11 | 63.2 |
| MsG0880042688.01.T01 | AT5G46800 | 26.606 | 218 | 122 | 7 | 132 | 341 | 109 | 296 | 5.07e-11 | 63.2 |
| MsG0880042688.01.T01 | AT1G14560 | 30.323 | 155 | 98 | 3 | 61 | 214 | 168 | 313 | 5.47e-11 | 63.5 |
| MsG0880042688.01.T01 | AT1G14560 | 21.192 | 302 | 194 | 7 | 51 | 341 | 49 | 317 | 7.83e-11 | 63.2 |
| MsG0880042688.01.T01 | AT4G01100 | 28.436 | 211 | 134 | 8 | 35 | 231 | 159 | 366 | 5.67e-11 | 63.5 |
Find 96 sgRNAs with CRISPR-Local
Find 114 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGGGTTTGTATAGAGGTTT+TGG | 0.224699 | 8:+12638701 | MsG0880042688.01.T01:CDS |
| CTGTTGCACAAAGGATGGTT+TGG | 0.306479 | 8:+12638777 | MsG0880042688.01.T01:CDS |
| CGGGTTTGGTACTTCGTTGA+TGG | 0.314901 | 8:+12638385 | MsG0880042688.01.T01:CDS |
| GGAACTGCTACTGTTGGTTT+TGG | 0.344381 | 8:+12638467 | MsG0880042688.01.T01:CDS |
| GATGCAGACGGTTAGAAGTT+TGG | 0.354056 | 8:+12639030 | MsG0880042688.01.T01:CDS |
| GCAATTGCTAATGCGGTTGC+TGG | 0.368970 | 8:+12638512 | MsG0880042688.01.T01:CDS |
| TGTTGCACAAAGGATGGTTT+GGG | 0.377065 | 8:+12638778 | MsG0880042688.01.T01:CDS |
| AGAGCGTTGTATCGCGGGTT+TGG | 0.379134 | 8:+12638371 | MsG0880042688.01.T01:CDS |
| TCGTCTGTAAGATATGTTAA+TGG | 0.389810 | 8:+12638638 | MsG0880042688.01.T01:CDS |
| CCCGGCTAGGGCGTTGTATA+TGG | 0.394032 | 8:+12638415 | MsG0880042688.01.T01:CDS |
| TAAAACCTCTTGATTCTTTG+CGG | 0.394234 | 8:-12639164 | None:intergenic |
| GGTTGGATGGCTTGTTATAG+AGG | 0.399503 | 8:+12639064 | MsG0880042688.01.T01:CDS |
| ATGGCTTGTTATAGAGGATT+GGG | 0.407744 | 8:+12639070 | MsG0880042688.01.T01:CDS |
| AGGATGGTTTGGGGTGGAGT+TGG | 0.412562 | 8:+12638788 | MsG0880042688.01.T01:CDS |
| ATGTTCCAGCTGAAATTGAT+TGG | 0.414525 | 8:+12638183 | MsG0880042688.01.T01:CDS |
| ATAGAGGATTGGGACCTAGA+TGG | 0.418086 | 8:+12639080 | MsG0880042688.01.T01:CDS |
| GGTCCTAAGGGTTTGTATAG+AGG | 0.421094 | 8:+12638695 | MsG0880042688.01.T01:CDS |
| TGCTTTGATTACTATGCCAT+TGG | 0.422067 | 8:+12638943 | MsG0880042688.01.T01:CDS |
| TACCTTGAACCATCAACCTT+TGG | 0.422147 | 8:-12638589 | None:intergenic |
| ACAAGGCTGCAGGTCTTAGA+TGG | 0.426779 | 8:+12638977 | MsG0880042688.01.T01:CDS |
| GATGGCTTGTTATAGAGGAT+TGG | 0.434742 | 8:+12639069 | MsG0880042688.01.T01:CDS |
| TGAGGAAGAGGTAGAGTCAG+AGG | 0.443657 | 8:+12638151 | MsG0880042688.01.T01:CDS |
| GGTTTAGAGCGTTGTATCGC+GGG | 0.444190 | 8:+12638366 | MsG0880042688.01.T01:CDS |
| GACGGTTAGAAGTTTGGTTA+AGG | 0.449500 | 8:+12639036 | MsG0880042688.01.T01:CDS |
| GGTTAAGGAAGGTGGTTGGA+TGG | 0.449945 | 8:+12639051 | MsG0880042688.01.T01:CDS |
| TTGTAATTCAAGTGATCCTA+AGG | 0.453755 | 8:+12638613 | MsG0880042688.01.T01:CDS |
| TGTTGTGAGCCAAAGGTTGA+TGG | 0.459063 | 8:+12638580 | MsG0880042688.01.T01:CDS |
| GCCATATACAACGCCCTAGC+CGG | 0.460196 | 8:-12638416 | None:intergenic |
| AGAAGTTTGGTTAAGGAAGG+TGG | 0.466995 | 8:+12639043 | MsG0880042688.01.T01:CDS |
| TTCGTTGATGGGTACGATCC+CGG | 0.468966 | 8:+12638397 | MsG0880042688.01.T01:CDS |
| CTGTTGTTGTGTTGAAAACT+AGG | 0.469626 | 8:+12638279 | MsG0880042688.01.T01:CDS |
| AGGAAAATTGTGAGAACGGA+TGG | 0.476248 | 8:+12638674 | MsG0880042688.01.T01:CDS |
| GGGTTTGGTACTTCGTTGAT+GGG | 0.476339 | 8:+12638386 | MsG0880042688.01.T01:CDS |
| GTTAGAAGTTTGGTTAAGGA+AGG | 0.488369 | 8:+12639040 | MsG0880042688.01.T01:CDS |
| CTTTGGCTCACAACATCAAT+CGG | 0.491438 | 8:-12638572 | None:intergenic |
| AAAACTGTTATGGCAGTTCA+GGG | 0.494556 | 8:+12638890 | MsG0880042688.01.T01:CDS |
| TGTGAGAACGGATGGTCCTA+AGG | 0.495628 | 8:+12638682 | MsG0880042688.01.T01:CDS |
| AGCCAAAGGTTGATGGTTCA+AGG | 0.498610 | 8:+12638587 | MsG0880042688.01.T01:CDS |
| AGCAGTTCCTACATTACTCT+TGG | 0.499952 | 8:-12638453 | None:intergenic |
| GTCTTAGATGGCGATGAGAA+TGG | 0.502262 | 8:+12638989 | MsG0880042688.01.T01:CDS |
| CCATATACAACGCCCTAGCC+GGG | 0.504449 | 8:-12638415 | None:intergenic |
| AAACCTCTATACAAACCCTT+AGG | 0.507700 | 8:-12638698 | None:intergenic |
| TTTCGTTGATTAAAGGGGAG+GGG | 0.510089 | 8:+12638345 | MsG0880042688.01.T01:CDS |
| TAGAGGATTGGGACCTAGAT+GGG | 0.510090 | 8:+12639081 | MsG0880042688.01.T01:CDS |
| CTCCCCTTTAATCAACGAAA+AGG | 0.512523 | 8:-12638342 | None:intergenic |
| GCCCACCATACAGCATTTGA+AGG | 0.513044 | 8:-12638746 | None:intergenic |
| TGATGGGTACGATCCCGGCT+AGG | 0.513383 | 8:+12638402 | MsG0880042688.01.T01:CDS |
| ATTTGCCAATCAATTTCAGC+TGG | 0.515337 | 8:-12638188 | None:intergenic |
| TTTGAGTGTGAATGAGGAAG+AGG | 0.516208 | 8:+12638139 | MsG0880042688.01.T01:CDS |
| ATATTCTGTTGCACAAAGGA+TGG | 0.521296 | 8:+12638772 | MsG0880042688.01.T01:CDS |
| GATGGGTACGATCCCGGCTA+GGG | 0.521692 | 8:+12638403 | MsG0880042688.01.T01:CDS |
| GGAATCAGTGCAGCAATGGC+AGG | 0.523793 | 8:+12638911 | MsG0880042688.01.T01:CDS |
| CTTTAGCAGCTCAGCTTGTG+TGG | 0.529736 | 8:+12638546 | MsG0880042688.01.T01:CDS |
| CGCCTTCAAATGCTGTATGG+TGG | 0.533829 | 8:+12638744 | MsG0880042688.01.T01:CDS |
| GTTTGGTTAAGGAAGGTGGT+TGG | 0.535300 | 8:+12639047 | MsG0880042688.01.T01:CDS |
| TACAATCAAGACAAGGCTGC+AGG | 0.537518 | 8:+12638967 | MsG0880042688.01.T01:CDS |
| AATGTAGGAACTGCTACTGT+TGG | 0.541302 | 8:+12638461 | MsG0880042688.01.T01:CDS |
| AACGACATTGAAGCCCATCT+AGG | 0.543587 | 8:-12639094 | None:intergenic |
| TATCTTACAGACGAACCCTT+AGG | 0.545681 | 8:-12638629 | None:intergenic |
| GAGGTTACCAAGAGTAATGT+AGG | 0.546410 | 8:+12638446 | MsG0880042688.01.T01:CDS |
| ACTTGAGTAACAAGAAAGGT+GGG | 0.552159 | 8:+12638816 | MsG0880042688.01.T01:CDS |
| GGAAGTGACAACAACAATGG+AGG | 0.554499 | 8:+12638842 | MsG0880042688.01.T01:CDS |
| CTTGTCTTGATTGTATCCAA+TGG | 0.562754 | 8:-12638959 | None:intergenic |
| GCCTTCAAATGCTGTATGGT+GGG | 0.563279 | 8:+12638745 | MsG0880042688.01.T01:CDS |
| TACTTGAGTAACAAGAAAGG+TGG | 0.572354 | 8:+12638815 | MsG0880042688.01.T01:CDS |
| TTAGCAATTGCAGCAGCCGT+CGG | 0.572422 | 8:-12638500 | None:intergenic |
| GGCGATGAGAATGGCCGTCG+TGG | 0.574613 | 8:+12638998 | MsG0880042688.01.T01:CDS |
| TCAGGGAATCAGTGCAGCAA+TGG | 0.574833 | 8:+12638907 | MsG0880042688.01.T01:CDS |
| ATGCGCCTTCAAATGCTGTA+TGG | 0.577473 | 8:+12638741 | MsG0880042688.01.T01:CDS |
| GGCTGCTGCAATTGCTAATG+CGG | 0.580901 | 8:+12638505 | MsG0880042688.01.T01:CDS |
| AATGGATTTGAGTGTGAATG+AGG | 0.583175 | 8:+12638133 | None:intergenic |
| GTGAGAACGGATGGTCCTAA+GGG | 0.584222 | 8:+12638683 | MsG0880042688.01.T01:CDS |
| GGGTTTAGAGCGTTGTATCG+CGG | 0.584544 | 8:+12638365 | MsG0880042688.01.T01:CDS |
| GTTGTATATGGCAGCGCTTG+AGG | 0.586997 | 8:+12638427 | MsG0880042688.01.T01:CDS |
| GAAGTGACAACAACAATGGA+GGG | 0.589871 | 8:+12638843 | MsG0880042688.01.T01:CDS |
| AGGGGCTAATGTGTTGAGGC+CGG | 0.590691 | 8:+12638862 | MsG0880042688.01.T01:CDS |
| TGTAATTCAAGTGATCCTAA+GGG | 0.603205 | 8:+12638614 | MsG0880042688.01.T01:CDS |
| GTTGCACAAAGGATGGTTTG+GGG | 0.609426 | 8:+12638779 | MsG0880042688.01.T01:CDS |
| CGATTGATGTTGTGAGCCAA+AGG | 0.611253 | 8:+12638573 | MsG0880042688.01.T01:CDS |
| CTTCATATTCTGTTGCACAA+AGG | 0.611809 | 8:+12638768 | MsG0880042688.01.T01:CDS |
| TATTACTTGAGTAACAAGAA+AGG | 0.614289 | 8:+12638812 | MsG0880042688.01.T01:CDS |
| ATCAGTGCAGCAATGGCAGG+TGG | 0.618720 | 8:+12638914 | MsG0880042688.01.T01:CDS |
| GCTTTCCGCAAAGAATCAAG+AGG | 0.618949 | 8:+12639159 | MsG0880042688.01.T01:CDS |
| AGTAACAAGAAAGGTGGGGA+GGG | 0.623934 | 8:+12638821 | MsG0880042688.01.T01:CDS |
| CAGAAAACAAAGCAGCACCA+AGG | 0.624753 | 8:-12638235 | None:intergenic |
| GAGGGAAGTGACAACAACAA+TGG | 0.626306 | 8:+12638839 | MsG0880042688.01.T01:CDS |
| GCACAAAGGATGGTTTGGGG+TGG | 0.629155 | 8:+12638782 | MsG0880042688.01.T01:CDS |
| GAGTAACAAGAAAGGTGGGG+AGG | 0.639195 | 8:+12638820 | MsG0880042688.01.T01:CDS |
| CTTGAGTAACAAGAAAGGTG+GGG | 0.640700 | 8:+12638817 | MsG0880042688.01.T01:CDS |
| ATGGAGGGGCTAATGTGTTG+AGG | 0.647603 | 8:+12638858 | MsG0880042688.01.T01:CDS |
| CTAACCGTCTGCATCACTGT+TGG | 0.653039 | 8:-12639022 | None:intergenic |
| TGCGGTTGCTGGTTTGACTG+CGG | 0.661120 | 8:+12638523 | MsG0880042688.01.T01:CDS |
| GCATCACTGTTGGTCCACGA+CGG | 0.662402 | 8:-12639012 | None:intergenic |
| CATTGGATACAATCAAGACA+AGG | 0.683298 | 8:+12638960 | MsG0880042688.01.T01:CDS |
| TGGACCAACAGTGATGCAGA+CGG | 0.713938 | 8:+12639018 | MsG0880042688.01.T01:CDS |
| AAGTGACAACAACAATGGAG+GGG | 0.716540 | 8:+12638844 | MsG0880042688.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TATTACTTGAGTAACAAGAA+AGG | + | Chr8:12638812-12638831 | MsG0880042688.01.T01:CDS | 25.0% |
| !!! | TTAATGGTATTGATGCTTTT+AGG | + | Chr8:12638654-12638673 | MsG0880042688.01.T01:CDS | 25.0% |
| !!! | TTTTAGGAAAATTGTGAGAA+CGG | + | Chr8:12638670-12638689 | MsG0880042688.01.T01:CDS | 25.0% |
| TAAAACCTCTTGATTCTTTG+CGG | - | Chr8:12639167-12639186 | None:intergenic | 30.0% | |
| TCGTCTGTAAGATATGTTAA+TGG | + | Chr8:12638638-12638657 | MsG0880042688.01.T01:CDS | 30.0% | |
| TGTAATTCAAGTGATCCTAA+GGG | + | Chr8:12638614-12638633 | MsG0880042688.01.T01:CDS | 30.0% | |
| TTGTAATTCAAGTGATCCTA+AGG | + | Chr8:12638613-12638632 | MsG0880042688.01.T01:CDS | 30.0% | |
| ! | AAATCCAAGTTTTTCTTCCT+TGG | + | Chr8:12638218-12638237 | MsG0880042688.01.T01:CDS | 30.0% |
| !! | CAACCTATGAGCTTTTAAAA+CGG | + | Chr8:12639137-12639156 | MsG0880042688.01.T01:CDS | 30.0% |
| AAAAACTGTTATGGCAGTTC+AGG | + | Chr8:12638889-12638908 | MsG0880042688.01.T01:CDS | 35.0% | |
| AAAACTGTTATGGCAGTTCA+GGG | + | Chr8:12638890-12638909 | MsG0880042688.01.T01:CDS | 35.0% | |
| AAACCTCTATACAAACCCTT+AGG | - | Chr8:12638701-12638720 | None:intergenic | 35.0% | |
| ACTTGAGTAACAAGAAAGGT+GGG | + | Chr8:12638816-12638835 | MsG0880042688.01.T01:CDS | 35.0% | |
| ATATTCTGTTGCACAAAGGA+TGG | + | Chr8:12638772-12638791 | MsG0880042688.01.T01:CDS | 35.0% | |
| ATGTTCCAGCTGAAATTGAT+TGG | + | Chr8:12638183-12638202 | MsG0880042688.01.T01:CDS | 35.0% | |
| ATTTGCCAATCAATTTCAGC+TGG | - | Chr8:12638191-12638210 | None:intergenic | 35.0% | |
| CATTGGATACAATCAAGACA+AGG | + | Chr8:12638960-12638979 | MsG0880042688.01.T01:CDS | 35.0% | |
| CTGTTGTTGTGTTGAAAACT+AGG | + | Chr8:12638279-12638298 | MsG0880042688.01.T01:CDS | 35.0% | |
| CTTCATATTCTGTTGCACAA+AGG | + | Chr8:12638768-12638787 | MsG0880042688.01.T01:CDS | 35.0% | |
| CTTGTCTTGATTGTATCCAA+TGG | - | Chr8:12638962-12638981 | None:intergenic | 35.0% | |
| GTTAGAAGTTTGGTTAAGGA+AGG | + | Chr8:12639040-12639059 | MsG0880042688.01.T01:CDS | 35.0% | |
| TACTTGAGTAACAAGAAAGG+TGG | + | Chr8:12638815-12638834 | MsG0880042688.01.T01:CDS | 35.0% | |
| TGCTTTGATTACTATGCCAT+TGG | + | Chr8:12638943-12638962 | MsG0880042688.01.T01:CDS | 35.0% | |
| ! | AAGGGTTTGTATAGAGGTTT+TGG | + | Chr8:12638701-12638720 | MsG0880042688.01.T01:CDS | 35.0% |
| ! | AGGGTTTGTATAGAGGTTTT+GGG | + | Chr8:12638702-12638721 | MsG0880042688.01.T01:CDS | 35.0% |
| ! | GCCATAACAGTTTTTGTATC+CGG | - | Chr8:12638884-12638903 | None:intergenic | 35.0% |
| !! | ACTGCCTTTTCGTTGATTAA+AGG | + | Chr8:12638338-12638357 | MsG0880042688.01.T01:CDS | 35.0% |
| !! | ATGGCTTGTTATAGAGGATT+GGG | + | Chr8:12639070-12639089 | MsG0880042688.01.T01:CDS | 35.0% |
| !! | CTAGTTTTCAACACAACAAC+AGG | - | Chr8:12638281-12638300 | None:intergenic | 35.0% |
| !! | CTGCCTTTTCGTTGATTAAA+GGG | + | Chr8:12638339-12638358 | MsG0880042688.01.T01:CDS | 35.0% |
| !! | TGCCTTTTCGTTGATTAAAG+GGG | + | Chr8:12638340-12638359 | MsG0880042688.01.T01:CDS | 35.0% |
| !! | TTTTCGTTGATTAAAGGGGA+GGG | + | Chr8:12638344-12638363 | MsG0880042688.01.T01:CDS | 35.0% |
| !!! | AAGCCGTTTTAAAAGCTCAT+AGG | - | Chr8:12639143-12639162 | None:intergenic | 35.0% |
| !!! | GTATAGAGGTTTTGGGATAT+CGG | + | Chr8:12638709-12638728 | MsG0880042688.01.T01:CDS | 35.0% |
| AAGTGACAACAACAATGGAG+GGG | + | Chr8:12638844-12638863 | MsG0880042688.01.T01:CDS | 40.0% | |
| AATGTAGGAACTGCTACTGT+TGG | + | Chr8:12638461-12638480 | MsG0880042688.01.T01:CDS | 40.0% | |
| AGAAGTTTGGTTAAGGAAGG+TGG | + | Chr8:12639043-12639062 | MsG0880042688.01.T01:CDS | 40.0% | |
| AGCACCAAGGAAGAAAAACT+TGG | - | Chr8:12638225-12638244 | None:intergenic | 40.0% | |
| AGCAGTTCCTACATTACTCT+TGG | - | Chr8:12638456-12638475 | None:intergenic | 40.0% | |
| AGGAAAATTGTGAGAACGGA+TGG | + | Chr8:12638674-12638693 | MsG0880042688.01.T01:CDS | 40.0% | |
| CTCCCCTTTAATCAACGAAA+AGG | - | Chr8:12638345-12638364 | None:intergenic | 40.0% | |
| CTTGAGTAACAAGAAAGGTG+GGG | + | Chr8:12638817-12638836 | MsG0880042688.01.T01:CDS | 40.0% | |
| GAAGTGACAACAACAATGGA+GGG | + | Chr8:12638843-12638862 | MsG0880042688.01.T01:CDS | 40.0% | |
| GACGGTTAGAAGTTTGGTTA+AGG | + | Chr8:12639036-12639055 | MsG0880042688.01.T01:CDS | 40.0% | |
| GAGGTTACCAAGAGTAATGT+AGG | + | Chr8:12638446-12638465 | MsG0880042688.01.T01:CDS | 40.0% | |
| GCCGGATACAAAAACTGTTA+TGG | + | Chr8:12638880-12638899 | MsG0880042688.01.T01:CDS | 40.0% | |
| TACCTTGAACCATCAACCTT+TGG | - | Chr8:12638592-12638611 | None:intergenic | 40.0% | |
| TATCTTACAGACGAACCCTT+AGG | - | Chr8:12638632-12638651 | None:intergenic | 40.0% | |
| TGTTGCACAAAGGATGGTTT+GGG | + | Chr8:12638778-12638797 | MsG0880042688.01.T01:CDS | 40.0% | |
| TTTGAGTGTGAATGAGGAAG+AGG | + | Chr8:12638139-12638158 | MsG0880042688.01.T01:CDS | 40.0% | |
| ! | CTTTGGCTCACAACATCAAT+CGG | - | Chr8:12638575-12638594 | None:intergenic | 40.0% |
| ! | TTTCGTTGATTAAAGGGGAG+GGG | + | Chr8:12638345-12638364 | MsG0880042688.01.T01:CDS | 40.0% |
| !! | CTTTTCGTTGATTAAAGGGG+AGG | + | Chr8:12638343-12638362 | MsG0880042688.01.T01:CDS | 40.0% |
| !! | GATGGCTTGTTATAGAGGAT+TGG | + | Chr8:12639069-12639088 | MsG0880042688.01.T01:CDS | 40.0% |
| AACGACATTGAAGCCCATCT+AGG | - | Chr8:12639097-12639116 | None:intergenic | 45.0% | |
| AGTAACAAGAAAGGTGGGGA+GGG | + | Chr8:12638821-12638840 | MsG0880042688.01.T01:CDS | 45.0% | |
| ATAGAGGATTGGGACCTAGA+TGG | + | Chr8:12639080-12639099 | MsG0880042688.01.T01:CDS | 45.0% | |
| ATGCGCCTTCAAATGCTGTA+TGG | + | Chr8:12638741-12638760 | MsG0880042688.01.T01:CDS | 45.0% | |
| CAGAAAACAAAGCAGCACCA+AGG | - | Chr8:12638238-12638257 | None:intergenic | 45.0% | |
| CGATTGATGTTGTGAGCCAA+AGG | + | Chr8:12638573-12638592 | MsG0880042688.01.T01:CDS | 45.0% | |
| CTGTTGCACAAAGGATGGTT+TGG | + | Chr8:12638777-12638796 | MsG0880042688.01.T01:CDS | 45.0% | |
| GAGGGAAGTGACAACAACAA+TGG | + | Chr8:12638839-12638858 | MsG0880042688.01.T01:CDS | 45.0% | |
| GATGCAGACGGTTAGAAGTT+TGG | + | Chr8:12639030-12639049 | MsG0880042688.01.T01:CDS | 45.0% | |
| GCCTTCAAATGCTGTATGGT+GGG | + | Chr8:12638745-12638764 | MsG0880042688.01.T01:CDS | 45.0% | |
| GCTTTCCGCAAAGAATCAAG+AGG | + | Chr8:12639159-12639178 | MsG0880042688.01.T01:CDS | 45.0% | |
| GGAAGTGACAACAACAATGG+AGG | + | Chr8:12638842-12638861 | MsG0880042688.01.T01:CDS | 45.0% | |
| GGTCCTAAGGGTTTGTATAG+AGG | + | Chr8:12638695-12638714 | MsG0880042688.01.T01:CDS | 45.0% | |
| GTCTTAGATGGCGATGAGAA+TGG | + | Chr8:12638989-12639008 | MsG0880042688.01.T01:CDS | 45.0% | |
| GTTGCACAAAGGATGGTTTG+GGG | + | Chr8:12638779-12638798 | MsG0880042688.01.T01:CDS | 45.0% | |
| GTTTGGTTAAGGAAGGTGGT+TGG | + | Chr8:12639047-12639066 | MsG0880042688.01.T01:CDS | 45.0% | |
| TACAATCAAGACAAGGCTGC+AGG | + | Chr8:12638967-12638986 | MsG0880042688.01.T01:CDS | 45.0% | |
| TAGAGGATTGGGACCTAGAT+GGG | + | Chr8:12639081-12639100 | MsG0880042688.01.T01:CDS | 45.0% | |
| TGTTGTGAGCCAAAGGTTGA+TGG | + | Chr8:12638580-12638599 | MsG0880042688.01.T01:CDS | 45.0% | |
| ! | GGGTTTGGTACTTCGTTGAT+GGG | + | Chr8:12638386-12638405 | MsG0880042688.01.T01:CDS | 45.0% |
| !! | AGCCAAAGGTTGATGGTTCA+AGG | + | Chr8:12638587-12638606 | MsG0880042688.01.T01:CDS | 45.0% |
| !! | GGTTGGATGGCTTGTTATAG+AGG | + | Chr8:12639064-12639083 | MsG0880042688.01.T01:CDS | 45.0% |
| !!! | CTTGGTGCTGCTTTGTTTTC+TGG | + | Chr8:12638236-12638255 | MsG0880042688.01.T01:CDS | 45.0% |
| !!! | GGAACTGCTACTGTTGGTTT+TGG | + | Chr8:12638467-12638486 | MsG0880042688.01.T01:CDS | 45.0% |
| !!! | TTTTGGTCTTGCTGAACCGA+CGG | + | Chr8:12638484-12638503 | MsG0880042688.01.T01:CDS | 45.0% |
| ACAAGGCTGCAGGTCTTAGA+TGG | + | Chr8:12638977-12638996 | MsG0880042688.01.T01:CDS | 50.0% | |
| ATGGAGGGGCTAATGTGTTG+AGG | + | Chr8:12638858-12638877 | MsG0880042688.01.T01:CDS | 50.0% | |
| CGCCTTCAAATGCTGTATGG+TGG | + | Chr8:12638744-12638763 | MsG0880042688.01.T01:CDS | 50.0% | |
| CTAACCGTCTGCATCACTGT+TGG | - | Chr8:12639025-12639044 | None:intergenic | 50.0% | |
| CTTTAGCAGCTCAGCTTGTG+TGG | + | Chr8:12638546-12638565 | MsG0880042688.01.T01:CDS | 50.0% | |
| GAGTAACAAGAAAGGTGGGG+AGG | + | Chr8:12638820-12638839 | MsG0880042688.01.T01:CDS | 50.0% | |
| GCAATTGCTAATGCGGTTGC+TGG | + | Chr8:12638512-12638531 | MsG0880042688.01.T01:CDS | 50.0% | |
| GCCCACCATACAGCATTTGA+AGG | - | Chr8:12638749-12638768 | None:intergenic | 50.0% | |
| GGCTGCTGCAATTGCTAATG+CGG | + | Chr8:12638505-12638524 | MsG0880042688.01.T01:CDS | 50.0% | |
| GGTTAAGGAAGGTGGTTGGA+TGG | + | Chr8:12639051-12639070 | MsG0880042688.01.T01:CDS | 50.0% | |
| GTGAGAACGGATGGTCCTAA+GGG | + | Chr8:12638683-12638702 | MsG0880042688.01.T01:CDS | 50.0% | |
| GTTGTATATGGCAGCGCTTG+AGG | + | Chr8:12638427-12638446 | MsG0880042688.01.T01:CDS | 50.0% | |
| TCAGGGAATCAGTGCAGCAA+TGG | + | Chr8:12638907-12638926 | MsG0880042688.01.T01:CDS | 50.0% | |
| TGAGGAAGAGGTAGAGTCAG+AGG | + | Chr8:12638151-12638170 | MsG0880042688.01.T01:CDS | 50.0% | |
| TGGACCAACAGTGATGCAGA+CGG | + | Chr8:12639018-12639037 | MsG0880042688.01.T01:CDS | 50.0% | |
| TGTGAGAACGGATGGTCCTA+AGG | + | Chr8:12638682-12638701 | MsG0880042688.01.T01:CDS | 50.0% | |
| TTAGCAATTGCAGCAGCCGT+CGG | - | Chr8:12638503-12638522 | None:intergenic | 50.0% | |
| ! | CGGGTTTGGTACTTCGTTGA+TGG | + | Chr8:12638385-12638404 | MsG0880042688.01.T01:CDS | 50.0% |
| ! | TTCGTTGATGGGTACGATCC+CGG | + | Chr8:12638397-12638416 | MsG0880042688.01.T01:CDS | 50.0% |
| !! | GGGTTTAGAGCGTTGTATCG+CGG | + | Chr8:12638365-12638384 | MsG0880042688.01.T01:CDS | 50.0% |
| !! | GGTTTAGAGCGTTGTATCGC+GGG | + | Chr8:12638366-12638385 | MsG0880042688.01.T01:CDS | 50.0% |
| AGGGGCTAATGTGTTGAGGC+CGG | + | Chr8:12638862-12638881 | MsG0880042688.01.T01:CDS | 55.0% | |
| ATCAGTGCAGCAATGGCAGG+TGG | + | Chr8:12638914-12638933 | MsG0880042688.01.T01:CDS | 55.0% | |
| CCATATACAACGCCCTAGCC+GGG | - | Chr8:12638418-12638437 | None:intergenic | 55.0% | |
| GCATCACTGTTGGTCCACGA+CGG | - | Chr8:12639015-12639034 | None:intergenic | 55.0% | |
| GCCATATACAACGCCCTAGC+CGG | - | Chr8:12638419-12638438 | None:intergenic | 55.0% | |
| GGAATCAGTGCAGCAATGGC+AGG | + | Chr8:12638911-12638930 | MsG0880042688.01.T01:CDS | 55.0% | |
| ! | AGGATGGTTTGGGGTGGAGT+TGG | + | Chr8:12638788-12638807 | MsG0880042688.01.T01:CDS | 55.0% |
| ! | GCACAAAGGATGGTTTGGGG+TGG | + | Chr8:12638782-12638801 | MsG0880042688.01.T01:CDS | 55.0% |
| !! | AGAGCGTTGTATCGCGGGTT+TGG | + | Chr8:12638371-12638390 | MsG0880042688.01.T01:CDS | 55.0% |
| !! | TGCGGTTGCTGGTTTGACTG+CGG | + | Chr8:12638523-12638542 | MsG0880042688.01.T01:CDS | 55.0% |
| GATGGGTACGATCCCGGCTA+GGG | + | Chr8:12638403-12638422 | MsG0880042688.01.T01:CDS | 60.0% | |
| ! | TGATGGGTACGATCCCGGCT+AGG | + | Chr8:12638402-12638421 | MsG0880042688.01.T01:CDS | 60.0% |
| !! | CCCGGCTAGGGCGTTGTATA+TGG | + | Chr8:12638415-12638434 | MsG0880042688.01.T01:CDS | 60.0% |
| GGCGATGAGAATGGCCGTCG+TGG | + | Chr8:12638998-12639017 | MsG0880042688.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 12638134 | 12639192 | 12638134 | ID=MsG0880042688.01;Name=MsG0880042688.01 |
| Chr8 | mRNA | 12638134 | 12639192 | 12638134 | ID=MsG0880042688.01.T01;Parent=MsG0880042688.01;Name=MsG0880042688.01.T01;_AED=0.38;_eAED=0.38;_QI=0|-1|0|1|-1|1|1|0|352 |
| Chr8 | exon | 12638134 | 12639192 | 12638134 | ID=MsG0880042688.01.T01:exon:798;Parent=MsG0880042688.01.T01 |
| Chr8 | CDS | 12638134 | 12639192 | 12638134 | ID=MsG0880042688.01.T01:cds;Parent=MsG0880042688.01.T01 |
| Gene Sequence |
| Protein sequence |
