Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023360.01.T01 | XP_003630087.1 | 96 | 75 | 3 | 0 | 1 | 75 | 1 | 75 | 1.30E-44 | 147 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023360.01.T01 | G7LAG4 | 96.000 | 75 | 3 | 0 | 1 | 75 | 1 | 75 | 6.21e-45 | 147 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280010663.01 | MsG0480023360.01 | 0.838467 | 2.826072e-57 | 3.788684e-54 |
| MsG0280010667.01 | MsG0480023360.01 | 0.803459 | 3.450506e-49 | 1.734928e-46 |
| MsG0480022362.01 | MsG0480023360.01 | 0.821498 | 3.909819e-53 | 3.186148e-50 |
| MsG0480023360.01 | MsG0780040645.01 | 0.819768 | 9.765508e-53 | 7.584747e-50 |
| MsG0480023360.01 | MsG0880042688.01 | 0.841086 | 5.879232e-58 | 8.543356e-55 |
| MsG0480023360.01 | MsG0880042860.01 | 0.806791 | 6.927457e-50 | 3.794253e-47 |
| MsG0480023360.01 | MsG0880045166.01 | 0.808817 | 2.569851e-50 | 1.484005e-47 |
| MsG0480023360.01 | MsG0880046170.01 | 0.828105 | 1.081373e-54 | 1.064520e-51 |
| MsG0380015539.01 | MsG0480023360.01 | 0.810349 | 1.205031e-50 | 7.246574e-48 |
| MsG0380016573.01 | MsG0480023360.01 | 0.816640 | 4.983197e-52 | 3.550498e-49 |
| MsG0380016707.01 | MsG0480023360.01 | 0.853075 | 3.036834e-61 | 6.481714e-58 |
| MsG0380017076.01 | MsG0480023360.01 | 0.848923 | 4.497476e-60 | 8.385257e-57 |
| MsG0380018060.01 | MsG0480023360.01 | 0.804050 | 2.602133e-49 | 1.328377e-46 |
| MsG0480018355.01 | MsG0480023360.01 | 0.816579 | 5.141442e-52 | 3.657225e-49 |
| MsG0280006606.01 | MsG0480023360.01 | 0.812390 | 4.342837e-51 | 2.757827e-48 |
| MsG0280006700.01 | MsG0480023360.01 | 0.825708 | 4.044864e-54 | 3.715387e-51 |
| MsG0280010047.01 | MsG0480023360.01 | 0.818997 | 1.463231e-52 | 1.112465e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023360.01.T01 | MTR_8g091590 | 96.000 | 75 | 3 | 0 | 1 | 75 | 1 | 75 | 1.58e-48 | 147 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 9 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCAATAGCAAATTGAGTTT+TGG | 0.254507 | 4:+84186369 | None:intergenic |
| GCAACTTGTTCAGAGTGTTT+TGG | 0.336230 | 4:-84186395 | MsG0480023360.01.T01:CDS |
| GTCTTCTTGTTATTCACTAC+TGG | 0.369369 | 4:-84186443 | MsG0480023360.01.T01:CDS |
| CTTCTTCTCCTCCTTCAACT+TGG | 0.390702 | 4:+84186417 | None:intergenic |
| ACTACTGGTTTCCAAGTTGA+AGG | 0.427104 | 4:-84186428 | MsG0480023360.01.T01:CDS |
| CATTGCTATGTTAGTAGCTG+AGG | 0.547311 | 4:-84186539 | MsG0480023360.01.T01:CDS |
| GTGGTGCTGCTGTAGAAACA+TGG | 0.583385 | 4:-84186477 | MsG0480023360.01.T01:CDS |
| ACTGGTTTCCAAGTTGAAGG+AGG | 0.631072 | 4:-84186425 | MsG0480023360.01.T01:CDS |
| TGGTGCTGCTGTAGAAACAT+GGG | 0.694208 | 4:-84186476 | MsG0480023360.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGAGTTTAAAAAATGCTGG+TGG | - | Chr4:84186390-84186409 | MsG0480023360.01.T01:CDS | 30.0% | |
| GTCAAGAGTTTAAAAAATGC+TGG | - | Chr4:84186387-84186406 | MsG0480023360.01.T01:CDS | 30.0% | |
| ! | AGCAATAGCAAATTGAGTTT+TGG | + | Chr4:84186523-84186542 | None:intergenic | 30.0% |
| ! | GCAATAGCAAATTGAGTTTT+GGG | + | Chr4:84186522-84186541 | None:intergenic | 30.0% |
| AGTTTAAAAAATGCTGGTGG+TGG | - | Chr4:84186393-84186412 | MsG0480023360.01.T01:CDS | 35.0% | |
| !! | GTCTTCTTGTTATTCACTAC+TGG | - | Chr4:84186446-84186465 | MsG0480023360.01.T01:CDS | 35.0% |
| CATTGCTATGTTAGTAGCTG+AGG | - | Chr4:84186350-84186369 | MsG0480023360.01.T01:CDS | 40.0% | |
| !! | ACTACTGGTTTCCAAGTTGA+AGG | - | Chr4:84186461-84186480 | MsG0480023360.01.T01:CDS | 40.0% |
| !! | GCAACTTGTTCAGAGTGTTT+TGG | - | Chr4:84186494-84186513 | MsG0480023360.01.T01:CDS | 40.0% |
| CTTCTTCTCCTCCTTCAACT+TGG | + | Chr4:84186475-84186494 | None:intergenic | 45.0% | |
| ! | ACTGGTTTCCAAGTTGAAGG+AGG | - | Chr4:84186464-84186483 | MsG0480023360.01.T01:CDS | 45.0% |
| !! | TGGTGCTGCTGTAGAAACAT+GGG | - | Chr4:84186413-84186432 | MsG0480023360.01.T01:CDS | 45.0% |
| !! | GTGGTGCTGCTGTAGAAACA+TGG | - | Chr4:84186412-84186431 | MsG0480023360.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 84186342 | 84186569 | 84186342 | ID=MsG0480023360.01;Name=MsG0480023360.01 |
| Chr4 | mRNA | 84186342 | 84186569 | 84186342 | ID=MsG0480023360.01.T01;Parent=MsG0480023360.01;Name=MsG0480023360.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|75 |
| Chr4 | exon | 84186342 | 84186569 | 84186342 | ID=MsG0480023360.01.T01:exon:61780;Parent=MsG0480023360.01.T01 |
| Chr4 | CDS | 84186342 | 84186569 | 84186342 | ID=MsG0480023360.01.T01:cds;Parent=MsG0480023360.01.T01 |
| Gene Sequence |
| Protein sequence |
