Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042729.01.T01 | XP_003627280.1 | 99.522 | 209 | 1 | 0 | 1 | 209 | 1 | 209 | 6.44E-148 | 419 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042729.01.T01 | Q9ZRA4 | 58.937 | 207 | 82 | 3 | 3 | 207 | 2 | 207 | 5.65E-78 | 235 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042729.01.T01 | G7L6V2 | 99.522 | 209 | 1 | 0 | 1 | 209 | 1 | 209 | 3.08e-148 | 419 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047999.01 | MsG0880042729.01 | 0.837927 | 3.892979e-57 | 5.133637e-54 |
| MsG0380014802.01 | MsG0880042729.01 | 0.839985 | 1.142042e-57 | 1.604221e-54 |
| MsG0680030545.01 | MsG0880042729.01 | 0.800561 | 1.360393e-48 | 6.354061e-46 |
| MsG0280008495.01 | MsG0880042729.01 | 0.806450 | 8.179278e-50 | 4.440853e-47 |
| MsG0280008553.01 | MsG0880042729.01 | 0.845362 | 4.259044e-59 | 7.080190e-56 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042729.01.T01 | MTR_8g020670 | 99.522 | 209 | 1 | 0 | 1 | 209 | 1 | 209 | 7.80e-152 | 419 |
| MsG0880042729.01.T01 | MTR_8g020630 | 88.038 | 209 | 25 | 0 | 1 | 209 | 1 | 209 | 6.90e-134 | 374 |
| MsG0880042729.01.T01 | MTR_8g020650 | 87.560 | 209 | 26 | 0 | 1 | 209 | 1 | 209 | 1.12e-126 | 356 |
| MsG0880042729.01.T01 | MTR_2g044040 | 72.249 | 209 | 58 | 0 | 1 | 209 | 1 | 209 | 5.16e-106 | 303 |
| MsG0880042729.01.T01 | MTR_2g045100 | 70.813 | 209 | 61 | 0 | 1 | 209 | 1 | 209 | 7.05e-104 | 298 |
| MsG0880042729.01.T01 | MTR_2g036120 | 70.048 | 207 | 62 | 0 | 1 | 207 | 1 | 207 | 6.62e-101 | 291 |
| MsG0880042729.01.T01 | MTR_3g019710 | 67.464 | 209 | 68 | 0 | 1 | 209 | 1 | 209 | 1.90e-98 | 285 |
| MsG0880042729.01.T01 | MTR_0284s0010 | 67.464 | 209 | 68 | 0 | 1 | 209 | 1 | 209 | 2.45e-98 | 284 |
| MsG0880042729.01.T01 | MTR_3g019650 | 66.986 | 209 | 68 | 1 | 1 | 209 | 1 | 208 | 1.80e-95 | 277 |
| MsG0880042729.01.T01 | MTR_3g019680 | 66.986 | 209 | 68 | 1 | 1 | 209 | 1 | 208 | 6.34e-95 | 275 |
| MsG0880042729.01.T01 | MTR_5g031460 | 65.072 | 209 | 73 | 0 | 1 | 209 | 1 | 209 | 1.30e-93 | 272 |
| MsG0880042729.01.T01 | MTR_7g011340 | 66.986 | 209 | 69 | 0 | 1 | 209 | 1 | 209 | 5.22e-90 | 263 |
| MsG0880042729.01.T01 | MTR_4g010340 | 61.836 | 207 | 76 | 2 | 3 | 209 | 2 | 205 | 1.77e-85 | 251 |
| MsG0880042729.01.T01 | MTR_3g019810 | 62.069 | 203 | 71 | 1 | 1 | 203 | 107 | 303 | 3.74e-79 | 239 |
| MsG0880042729.01.T01 | MTR_8g020590 | 57.767 | 206 | 84 | 3 | 6 | 209 | 4 | 208 | 5.38e-79 | 235 |
| MsG0880042729.01.T01 | MTR_2g041550 | 55.502 | 209 | 89 | 3 | 3 | 209 | 4 | 210 | 1.88e-76 | 229 |
| MsG0880042729.01.T01 | MTR_2g041580 | 55.024 | 209 | 90 | 3 | 3 | 209 | 4 | 210 | 2.98e-76 | 228 |
| MsG0880042729.01.T01 | MTR_8g020610 | 56.311 | 206 | 86 | 3 | 6 | 209 | 4 | 207 | 3.43e-76 | 228 |
| MsG0880042729.01.T01 | MTR_2g041570 | 54.545 | 209 | 91 | 3 | 3 | 209 | 4 | 210 | 2.40e-75 | 226 |
| MsG0880042729.01.T01 | MTR_2g019780 | 53.488 | 215 | 91 | 3 | 1 | 209 | 1 | 212 | 3.29e-75 | 226 |
| MsG0880042729.01.T01 | MTR_3g073170 | 56.522 | 207 | 84 | 3 | 6 | 209 | 7 | 210 | 1.08e-74 | 224 |
| MsG0880042729.01.T01 | MTR_8g020760 | 54.976 | 211 | 93 | 2 | 1 | 209 | 1 | 211 | 7.16e-74 | 222 |
| MsG0880042729.01.T01 | MTR_8g020770 | 50.239 | 209 | 102 | 2 | 3 | 209 | 4 | 212 | 2.69e-69 | 211 |
| MsG0880042729.01.T01 | MTR_8g020763 | 53.846 | 182 | 83 | 1 | 29 | 209 | 9 | 190 | 3.82e-66 | 202 |
| MsG0880042729.01.T01 | MTR_8g020750 | 48.804 | 209 | 105 | 2 | 3 | 209 | 4 | 212 | 1.48e-63 | 196 |
| MsG0880042729.01.T01 | MTR_4g027415 | 66.901 | 142 | 47 | 0 | 1 | 142 | 1 | 142 | 1.69e-55 | 173 |
| MsG0880042729.01.T01 | MTR_2g072560 | 66.957 | 115 | 38 | 0 | 1 | 115 | 1 | 115 | 7.16e-50 | 159 |
| MsG0880042729.01.T01 | MTR_3g019610 | 65.094 | 106 | 37 | 0 | 1 | 106 | 1 | 106 | 9.57e-44 | 142 |
| MsG0880042729.01.T01 | MTR_7g111240 | 37.500 | 192 | 116 | 3 | 21 | 208 | 23 | 214 | 1.17e-36 | 127 |
| MsG0880042729.01.T01 | MTR_7g111240 | 37.500 | 192 | 113 | 4 | 21 | 208 | 23 | 211 | 1.19e-35 | 125 |
| MsG0880042729.01.T01 | MTR_1g085140 | 35.602 | 191 | 119 | 3 | 22 | 208 | 28 | 218 | 2.40e-35 | 124 |
| MsG0880042729.01.T01 | MTR_1g085500 | 33.854 | 192 | 123 | 3 | 21 | 208 | 26 | 217 | 2.50e-33 | 119 |
| MsG0880042729.01.T01 | MTR_2g031270 | 35.079 | 191 | 115 | 4 | 21 | 205 | 26 | 213 | 1.92e-31 | 114 |
| MsG0880042729.01.T01 | MTR_1g079490 | 34.634 | 205 | 122 | 4 | 13 | 205 | 13 | 217 | 2.69e-31 | 114 |
| MsG0880042729.01.T01 | MTR_1g112200 | 33.990 | 203 | 125 | 5 | 6 | 205 | 11 | 207 | 4.27e-31 | 113 |
| MsG0880042729.01.T01 | MTR_6g005310 | 36.916 | 214 | 122 | 6 | 4 | 205 | 3 | 215 | 5.36e-31 | 113 |
| MsG0880042729.01.T01 | MTR_6g005330 | 38.220 | 191 | 110 | 4 | 22 | 205 | 26 | 215 | 6.43e-31 | 113 |
| MsG0880042729.01.T01 | MTR_2g030855 | 35.079 | 191 | 115 | 4 | 21 | 205 | 25 | 212 | 1.20e-30 | 112 |
| MsG0880042729.01.T01 | MTR_6g005360 | 38.220 | 191 | 110 | 4 | 22 | 205 | 26 | 215 | 1.29e-30 | 112 |
| MsG0880042729.01.T01 | MTR_6g005340 | 36.449 | 214 | 123 | 6 | 4 | 205 | 3 | 215 | 2.22e-30 | 111 |
| MsG0880042729.01.T01 | MTR_4g017040 | 35.023 | 217 | 126 | 6 | 1 | 204 | 1 | 215 | 2.40e-30 | 111 |
| MsG0880042729.01.T01 | MTR_1g060120 | 33.019 | 212 | 130 | 4 | 6 | 205 | 10 | 221 | 2.63e-30 | 111 |
| MsG0880042729.01.T01 | MTR_4g017050 | 35.160 | 219 | 123 | 7 | 1 | 204 | 1 | 215 | 3.38e-30 | 111 |
| MsG0880042729.01.T01 | MTR_5g046410 | 35.160 | 219 | 123 | 7 | 1 | 204 | 1 | 215 | 5.01e-30 | 110 |
| MsG0880042729.01.T01 | MTR_5g046430 | 35.160 | 219 | 123 | 7 | 1 | 204 | 1 | 215 | 5.01e-30 | 110 |
| MsG0880042729.01.T01 | MTR_4g017030 | 35.160 | 219 | 123 | 7 | 1 | 204 | 1 | 215 | 5.01e-30 | 110 |
| MsG0880042729.01.T01 | MTR_2g019250 | 34.562 | 217 | 127 | 6 | 1 | 204 | 1 | 215 | 7.06e-30 | 110 |
| MsG0880042729.01.T01 | MTR_1g023770 | 32.367 | 207 | 128 | 4 | 5 | 205 | 9 | 209 | 1.96e-29 | 108 |
| MsG0880042729.01.T01 | MTR_6g005380 | 36.500 | 200 | 112 | 5 | 9 | 194 | 7 | 205 | 1.42e-27 | 104 |
| MsG0880042729.01.T01 | MTR_5g036570 | 31.937 | 191 | 125 | 4 | 22 | 207 | 31 | 221 | 1.87e-27 | 104 |
| MsG0880042729.01.T01 | MTR_2g030895 | 34.940 | 166 | 101 | 4 | 47 | 205 | 13 | 178 | 4.55e-26 | 99.4 |
| MsG0880042729.01.T01 | MTR_2g086630 | 37.129 | 202 | 116 | 6 | 9 | 205 | 9 | 204 | 1.49e-25 | 99.0 |
| MsG0880042729.01.T01 | MTR_6g005350 | 33.491 | 212 | 128 | 5 | 6 | 205 | 4 | 214 | 2.85e-25 | 98.6 |
| MsG0880042729.01.T01 | MTR_2g086620 | 36.082 | 194 | 115 | 5 | 17 | 205 | 15 | 204 | 9.27e-25 | 96.7 |
| MsG0880042729.01.T01 | MTR_2g030865 | 34.940 | 166 | 101 | 4 | 47 | 205 | 13 | 178 | 1.47e-24 | 95.5 |
| MsG0880042729.01.T01 | MTR_8g007300 | 36.090 | 133 | 84 | 1 | 38 | 169 | 48 | 180 | 1.88e-24 | 96.3 |
| MsG0880042729.01.T01 | MTR_3g096330 | 34.211 | 152 | 97 | 2 | 21 | 169 | 580 | 731 | 5.38e-23 | 96.7 |
| MsG0880042729.01.T01 | MTR_2g086640 | 39.695 | 131 | 77 | 2 | 77 | 205 | 73 | 203 | 2.17e-22 | 90.5 |
| MsG0880042729.01.T01 | MTR_2g031300 | 42.000 | 100 | 54 | 2 | 47 | 142 | 13 | 112 | 1.64e-19 | 81.6 |
| MsG0880042729.01.T01 | MTR_2g096110 | 37.324 | 142 | 72 | 3 | 40 | 178 | 2 | 129 | 4.25e-19 | 80.1 |
| MsG0880042729.01.T01 | MTR_4g052780 | 35.556 | 135 | 85 | 2 | 73 | 205 | 71 | 205 | 4.94e-18 | 79.0 |
| MsG0880042729.01.T01 | MTR_4g052770 | 34.815 | 135 | 86 | 2 | 73 | 205 | 71 | 205 | 1.40e-17 | 77.8 |
| MsG0880042729.01.T01 | MTR_7g029500 | 56.604 | 53 | 23 | 0 | 150 | 202 | 38 | 90 | 5.32e-14 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042729.01.T01 | AT1G72610 | 58.854 | 192 | 77 | 2 | 20 | 209 | 17 | 208 | 2.59e-76 | 228 |
| MsG0880042729.01.T01 | AT5G20630 | 54.545 | 209 | 93 | 2 | 1 | 207 | 1 | 209 | 3.56e-75 | 226 |
| MsG0880042729.01.T01 | AT5G39190 | 37.557 | 221 | 122 | 6 | 1 | 206 | 1 | 220 | 5.01e-40 | 136 |
| MsG0880042729.01.T01 | AT5G39160 | 38.140 | 215 | 117 | 6 | 3 | 206 | 7 | 216 | 1.14e-39 | 135 |
| MsG0880042729.01.T01 | AT5G39110 | 38.636 | 220 | 120 | 5 | 1 | 205 | 1 | 220 | 1.25e-39 | 135 |
| MsG0880042729.01.T01 | AT5G39160 | 38.140 | 215 | 121 | 6 | 3 | 206 | 7 | 220 | 1.66e-39 | 135 |
| MsG0880042729.01.T01 | AT5G39130 | 37.104 | 221 | 123 | 6 | 1 | 206 | 1 | 220 | 6.04e-39 | 134 |
| MsG0880042729.01.T01 | AT3G04200 | 38.679 | 212 | 110 | 7 | 12 | 206 | 15 | 223 | 3.36e-38 | 132 |
| MsG0880042729.01.T01 | AT3G62020 | 36.967 | 211 | 123 | 6 | 8 | 208 | 8 | 218 | 3.39e-38 | 132 |
| MsG0880042729.01.T01 | AT1G09560 | 38.889 | 198 | 117 | 3 | 12 | 205 | 18 | 215 | 4.13e-38 | 131 |
| MsG0880042729.01.T01 | AT3G05930 | 36.493 | 211 | 126 | 4 | 3 | 205 | 5 | 215 | 6.09e-38 | 131 |
| MsG0880042729.01.T01 | AT3G05950 | 39.713 | 209 | 110 | 6 | 12 | 205 | 14 | 221 | 8.29e-38 | 131 |
| MsG0880042729.01.T01 | AT1G18980 | 36.765 | 204 | 123 | 4 | 6 | 205 | 17 | 218 | 9.72e-38 | 130 |
| MsG0880042729.01.T01 | AT5G39180 | 37.900 | 219 | 122 | 5 | 1 | 205 | 1 | 219 | 5.07e-37 | 129 |
| MsG0880042729.01.T01 | AT5G39150 | 37.900 | 219 | 122 | 5 | 1 | 205 | 1 | 219 | 1.97e-36 | 127 |
| MsG0880042729.01.T01 | AT1G02335 | 35.961 | 203 | 124 | 4 | 12 | 208 | 16 | 218 | 2.49e-36 | 127 |
| MsG0880042729.01.T01 | AT5G39120 | 38.356 | 219 | 121 | 6 | 1 | 205 | 1 | 219 | 4.55e-36 | 126 |
| MsG0880042729.01.T01 | AT5G26700 | 35.122 | 205 | 130 | 3 | 6 | 208 | 9 | 212 | 5.73e-36 | 125 |
| MsG0880042729.01.T01 | AT5G39190 | 35.849 | 212 | 116 | 4 | 1 | 206 | 1 | 198 | 8.13e-36 | 125 |
| MsG0880042729.01.T01 | AT3G62020 | 38.068 | 176 | 104 | 4 | 38 | 208 | 14 | 189 | 1.83e-35 | 124 |
| MsG0880042729.01.T01 | AT5G39160 | 35.377 | 212 | 117 | 4 | 1 | 206 | 1 | 198 | 2.57e-35 | 124 |
| MsG0880042729.01.T01 | AT5G38960 | 36.548 | 197 | 114 | 5 | 20 | 206 | 28 | 223 | 4.08e-34 | 121 |
| MsG0880042729.01.T01 | AT1G18970 | 35.323 | 201 | 124 | 4 | 9 | 205 | 20 | 218 | 4.96e-33 | 118 |
| MsG0880042729.01.T01 | AT3G10080 | 37.755 | 196 | 104 | 7 | 22 | 205 | 30 | 219 | 2.16e-32 | 117 |
| MsG0880042729.01.T01 | AT3G04150 | 33.163 | 196 | 123 | 4 | 22 | 209 | 30 | 225 | 1.36e-31 | 115 |
| MsG0880042729.01.T01 | AT3G10080 | 37.755 | 196 | 104 | 7 | 22 | 205 | 101 | 290 | 1.57e-31 | 116 |
| MsG0880042729.01.T01 | AT4G14630 | 35.238 | 210 | 124 | 6 | 7 | 205 | 14 | 222 | 2.38e-31 | 114 |
| MsG0880042729.01.T01 | AT3G04150 | 34.444 | 180 | 111 | 3 | 37 | 209 | 59 | 238 | 2.70e-31 | 114 |
| MsG0880042729.01.T01 | AT5G38910 | 35.981 | 214 | 127 | 5 | 1 | 205 | 7 | 219 | 1.99e-30 | 112 |
| MsG0880042729.01.T01 | AT1G74820 | 34.225 | 187 | 117 | 4 | 23 | 205 | 41 | 225 | 3.75e-29 | 108 |
| MsG0880042729.01.T01 | AT5G61750 | 36.000 | 175 | 96 | 4 | 6 | 170 | 7 | 175 | 5.50e-29 | 107 |
| MsG0880042729.01.T01 | AT3G04170 | 33.803 | 213 | 121 | 7 | 7 | 205 | 12 | 218 | 1.60e-28 | 107 |
| MsG0880042729.01.T01 | AT5G38940 | 35.052 | 194 | 118 | 4 | 20 | 205 | 28 | 221 | 2.15e-28 | 106 |
| MsG0880042729.01.T01 | AT5G38940 | 35.052 | 194 | 118 | 4 | 20 | 205 | 52 | 245 | 3.95e-28 | 106 |
| MsG0880042729.01.T01 | AT5G38930 | 34.536 | 194 | 119 | 4 | 20 | 205 | 28 | 221 | 1.61e-27 | 104 |
| MsG0880042729.01.T01 | AT3G04190 | 31.959 | 194 | 124 | 4 | 20 | 205 | 28 | 221 | 1.75e-27 | 104 |
| MsG0880042729.01.T01 | AT3G04180 | 31.959 | 194 | 124 | 4 | 20 | 205 | 28 | 221 | 1.30e-25 | 99.4 |
| MsG0880042729.01.T01 | AT1G10460 | 36.220 | 127 | 78 | 3 | 60 | 183 | 65 | 191 | 1.97e-18 | 80.5 |
| MsG0880042729.01.T01 | AT5G38910 | 29.384 | 211 | 108 | 5 | 1 | 205 | 7 | 182 | 4.97e-15 | 70.5 |
Find 46 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGATAGCCTGTAGGTGTTTC+TGG | 0.183963 | 8:-13285420 | None:intergenic |
| TGCTACCGAATTATTGATTA+TGG | 0.216281 | 8:+13285641 | MsG0880042729.01.T01:CDS |
| GAAAGAGTTGTTAGTGTTTC+CGG | 0.229368 | 8:-13285499 | None:intergenic |
| GAATTATTGATTATGGTTAA+AGG | 0.256071 | 8:+13285648 | MsG0880042729.01.T01:CDS |
| TAATTCAGAAGGTAAATTAT+TGG | 0.286814 | 8:-13285859 | None:intergenic |
| TTTCCAGCTCTTAACGATCT+TGG | 0.306429 | 8:+13285555 | MsG0880042729.01.T01:CDS |
| TAATTTACCTTCTGAATTAG+TGG | 0.324310 | 8:+13285863 | MsG0880042729.01.T01:CDS |
| GTTGAAACCGGGTGATCTTA+TGG | 0.324992 | 8:+13285719 | MsG0880042729.01.T01:CDS |
| AAGAAGCTAAAGGCTCGGTT+TGG | 0.389106 | 8:+13285918 | MsG0880042729.01.T01:CDS |
| TTATTCGAAGACGTTGAAAC+CGG | 0.390433 | 8:+13285707 | MsG0880042729.01.T01:CDS |
| TTTAACCATAATCAATAATT+CGG | 0.394261 | 8:-13285646 | None:intergenic |
| TCAGATGATTTCGTCTTTCA+CGG | 0.399030 | 8:+13285468 | MsG0880042729.01.T01:CDS |
| GTCTTTCACGGCTTTGTAGC+CGG | 0.402040 | 8:+13285480 | MsG0880042729.01.T01:CDS |
| AGAAGCTAAAGGCTCGGTTT+GGG | 0.424746 | 8:+13285919 | MsG0880042729.01.T01:CDS |
| TCTTCACTTGTGCAAGATCA+AGG | 0.425262 | 8:-13285899 | None:intergenic |
| ATCGTTAAGAGCTGGAAAAT+CGG | 0.453877 | 8:-13285550 | None:intergenic |
| TTTCAAGTTAATTCAGGTAA+GGG | 0.490682 | 8:+13285765 | MsG0880042729.01.T01:CDS |
| GTTAAAGGTGAAATAACTGC+TGG | 0.507279 | 8:+13285663 | MsG0880042729.01.T01:CDS |
| AGAGTGAAAAGGAAAAGTAT+AGG | 0.514106 | 8:-13285336 | None:intergenic |
| ATTCCAAGATCGTTAAGAGC+TGG | 0.523170 | 8:-13285558 | None:intergenic |
| ATCAATAATTCGGTAGCACC+AGG | 0.528604 | 8:-13285636 | None:intergenic |
| TATTCGAAGACGTTGAAACC+GGG | 0.533516 | 8:+13285708 | MsG0880042729.01.T01:CDS |
| AGATCAAGTAATTGAGCACC+AGG | 0.545978 | 8:-13285825 | None:intergenic |
| ATTCCAATGCATACTCATCC+TGG | 0.548743 | 8:+13285618 | MsG0880042729.01.T01:CDS |
| GCACCAGGATGAGTATGCAT+TGG | 0.553990 | 8:-13285621 | None:intergenic |
| GAAGCTAAAGGCTCGGTTTG+GGG | 0.563781 | 8:+13285920 | MsG0880042729.01.T01:CDS |
| AAAATCATGGACAGAAGCAT+AGG | 0.563955 | 8:-13285376 | None:intergenic |
| GTTTGTGCCACTAATTCAGA+AGG | 0.574335 | 8:-13285870 | None:intergenic |
| CATCTGATGTGATGTTTGCA+AGG | 0.576393 | 8:-13285452 | None:intergenic |
| AAGCTAAAGGCTCGGTTTGG+GGG | 0.585774 | 8:+13285921 | MsG0880042729.01.T01:CDS |
| TGCACAAGTGAAGAAGCTAA+AGG | 0.586569 | 8:+13285908 | MsG0880042729.01.T01:CDS |
| AAAGCTCCAGAAACACCTAC+AGG | 0.587844 | 8:+13285414 | MsG0880042729.01.T01:CDS |
| GACAGAAGCATAGGAAGTGG+TGG | 0.591518 | 8:-13285367 | None:intergenic |
| ATGGACAGAAGCATAGGAAG+TGG | 0.593850 | 8:-13285370 | None:intergenic |
| TGAAAATGCAACATCCCTTG+TGG | 0.594793 | 8:-13285747 | None:intergenic |
| ACTAAAAGTAAGAAAAGCAG+TGG | 0.611069 | 8:-13285793 | None:intergenic |
| ATCTGATGTGATGTTTGCAA+GGG | 0.619647 | 8:-13285451 | None:intergenic |
| TGGATGAGAGAAGAGTGAAA+AGG | 0.620855 | 8:-13285347 | None:intergenic |
| AAGTGAAGAAGCTAAAGGCT+CGG | 0.623995 | 8:+13285913 | MsG0880042729.01.T01:CDS |
| ATCTTGGAATCTCTGCGGCA+CGG | 0.632838 | 8:+13285571 | MsG0880042729.01.T01:CDS |
| GCACGGTTAGACATAGCGAA+AGG | 0.667594 | 8:+13285588 | MsG0880042729.01.T01:CDS |
| CGGTTAGACATAGCGAAAGG+CGG | 0.694402 | 8:+13285591 | MsG0880042729.01.T01:CDS |
| GGCATGCAGTGATAGCCTGT+AGG | 0.702564 | 8:-13285429 | None:intergenic |
| TAACGATCTTGGAATCTCTG+CGG | 0.726837 | 8:+13285566 | MsG0880042729.01.T01:CDS |
| TCTGATGTGATGTTTGCAAG+GGG | 0.732037 | 8:-13285450 | None:intergenic |
| AAAATCGGTGACAAACGCAG+AGG | 0.761602 | 8:-13285535 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTAACCATAATCAATAATT+CGG | - | Chr8:13285649-13285668 | None:intergenic | 15.0% |
| !! | TAATTCAGAAGGTAAATTAT+TGG | - | Chr8:13285862-13285881 | None:intergenic | 20.0% |
| !!! | GAATTATTGATTATGGTTAA+AGG | + | Chr8:13285648-13285667 | MsG0880042729.01.T01:CDS | 20.0% |
| ! | TAATTTACCTTCTGAATTAG+TGG | + | Chr8:13285863-13285882 | MsG0880042729.01.T01:CDS | 25.0% |
| !!! | TTGCATTTTCAAGTTAATTC+AGG | + | Chr8:13285759-13285778 | MsG0880042729.01.T01:CDS | 25.0% |
| !!! | TTTCAAGTTAATTCAGGTAA+GGG | + | Chr8:13285765-13285784 | MsG0880042729.01.T01:CDS | 25.0% |
| !!! | TTTTCAAGTTAATTCAGGTA+AGG | + | Chr8:13285764-13285783 | MsG0880042729.01.T01:CDS | 25.0% |
| ACTAAAAGTAAGAAAAGCAG+TGG | - | Chr8:13285796-13285815 | None:intergenic | 30.0% | |
| TATCTCCTACACAAAAATCA+TGG | - | Chr8:13285392-13285411 | None:intergenic | 30.0% | |
| ! | AGAGTGAAAAGGAAAAGTAT+AGG | - | Chr8:13285339-13285358 | None:intergenic | 30.0% |
| ! | ATCTTATGGTTTTTCCACAA+GGG | + | Chr8:13285733-13285752 | MsG0880042729.01.T01:CDS | 30.0% |
| ! | TGCTACCGAATTATTGATTA+TGG | + | Chr8:13285641-13285660 | MsG0880042729.01.T01:CDS | 30.0% |
| ATCGTTAAGAGCTGGAAAAT+CGG | - | Chr8:13285553-13285572 | None:intergenic | 35.0% | |
| ATCTGATGTGATGTTTGCAA+GGG | - | Chr8:13285454-13285473 | None:intergenic | 35.0% | |
| GAAAGAGTTGTTAGTGTTTC+CGG | - | Chr8:13285502-13285521 | None:intergenic | 35.0% | |
| GTTAAAGGTGAAATAACTGC+TGG | + | Chr8:13285663-13285682 | MsG0880042729.01.T01:CDS | 35.0% | |
| TCAGATGATTTCGTCTTTCA+CGG | + | Chr8:13285468-13285487 | MsG0880042729.01.T01:CDS | 35.0% | |
| TTATTCGAAGACGTTGAAAC+CGG | + | Chr8:13285707-13285726 | MsG0880042729.01.T01:CDS | 35.0% | |
| ! | AAAATCATGGACAGAAGCAT+AGG | - | Chr8:13285379-13285398 | None:intergenic | 35.0% |
| ! | ACTTTTAGTAGTGCAAATCC+TGG | + | Chr8:13285807-13285826 | MsG0880042729.01.T01:CDS | 35.0% |
| ! | GATCTTATGGTTTTTCCACA+AGG | + | Chr8:13285732-13285751 | MsG0880042729.01.T01:CDS | 35.0% |
| ! | TCTGTCCATGATTTTTGTGT+AGG | + | Chr8:13285384-13285403 | MsG0880042729.01.T01:CDS | 35.0% |
| AAGTGAAGAAGCTAAAGGCT+CGG | + | Chr8:13285913-13285932 | MsG0880042729.01.T01:CDS | 40.0% | |
| AGATCAAGTAATTGAGCACC+AGG | - | Chr8:13285828-13285847 | None:intergenic | 40.0% | |
| ATCAATAATTCGGTAGCACC+AGG | - | Chr8:13285639-13285658 | None:intergenic | 40.0% | |
| ATTCCAAGATCGTTAAGAGC+TGG | - | Chr8:13285561-13285580 | None:intergenic | 40.0% | |
| ATTCCAATGCATACTCATCC+TGG | + | Chr8:13285618-13285637 | MsG0880042729.01.T01:CDS | 40.0% | |
| CATCTGATGTGATGTTTGCA+AGG | - | Chr8:13285455-13285474 | None:intergenic | 40.0% | |
| GGAAAAACCATAAGATCACC+CGG | - | Chr8:13285729-13285748 | None:intergenic | 40.0% | |
| TAACGATCTTGGAATCTCTG+CGG | + | Chr8:13285566-13285585 | MsG0880042729.01.T01:CDS | 40.0% | |
| TATTCGAAGACGTTGAAACC+GGG | + | Chr8:13285708-13285727 | MsG0880042729.01.T01:CDS | 40.0% | |
| TCTGATGTGATGTTTGCAAG+GGG | - | Chr8:13285453-13285472 | None:intergenic | 40.0% | |
| TCTTCACTTGTGCAAGATCA+AGG | - | Chr8:13285902-13285921 | None:intergenic | 40.0% | |
| TGAAAATGCAACATCCCTTG+TGG | - | Chr8:13285750-13285769 | None:intergenic | 40.0% | |
| TGCACAAGTGAAGAAGCTAA+AGG | + | Chr8:13285908-13285927 | MsG0880042729.01.T01:CDS | 40.0% | |
| TGGATGAGAGAAGAGTGAAA+AGG | - | Chr8:13285350-13285369 | None:intergenic | 40.0% | |
| TTTCCAGCTCTTAACGATCT+TGG | + | Chr8:13285555-13285574 | MsG0880042729.01.T01:CDS | 40.0% | |
| ! | GTTTGTGCCACTAATTCAGA+AGG | - | Chr8:13285873-13285892 | None:intergenic | 40.0% |
| AAAATCGGTGACAAACGCAG+AGG | - | Chr8:13285538-13285557 | None:intergenic | 45.0% | |
| AAAGCTCCAGAAACACCTAC+AGG | + | Chr8:13285414-13285433 | MsG0880042729.01.T01:CDS | 45.0% | |
| GTTGAAACCGGGTGATCTTA+TGG | + | Chr8:13285719-13285738 | MsG0880042729.01.T01:CDS | 45.0% | |
| TGATAGCCTGTAGGTGTTTC+TGG | - | Chr8:13285423-13285442 | None:intergenic | 45.0% | |
| ! | AAGAAGCTAAAGGCTCGGTT+TGG | + | Chr8:13285918-13285937 | MsG0880042729.01.T01:CDS | 45.0% |
| ! | AGAAGCTAAAGGCTCGGTTT+GGG | + | Chr8:13285919-13285938 | MsG0880042729.01.T01:CDS | 45.0% |
| ! | ATGGACAGAAGCATAGGAAG+TGG | - | Chr8:13285373-13285392 | None:intergenic | 45.0% |
| ATCTTGGAATCTCTGCGGCA+CGG | + | Chr8:13285571-13285590 | MsG0880042729.01.T01:CDS | 50.0% | |
| CGGTTAGACATAGCGAAAGG+CGG | + | Chr8:13285591-13285610 | MsG0880042729.01.T01:CDS | 50.0% | |
| GCACCAGGATGAGTATGCAT+TGG | - | Chr8:13285624-13285643 | None:intergenic | 50.0% | |
| GCACGGTTAGACATAGCGAA+AGG | + | Chr8:13285588-13285607 | MsG0880042729.01.T01:CDS | 50.0% | |
| ! | GAAGCTAAAGGCTCGGTTTG+GGG | + | Chr8:13285920-13285939 | MsG0880042729.01.T01:CDS | 50.0% |
| ! | GACAGAAGCATAGGAAGTGG+TGG | - | Chr8:13285370-13285389 | None:intergenic | 50.0% |
| ! | GTCTTTCACGGCTTTGTAGC+CGG | + | Chr8:13285480-13285499 | MsG0880042729.01.T01:CDS | 50.0% |
| !! | AAGCTAAAGGCTCGGTTTGG+GGG | + | Chr8:13285921-13285940 | MsG0880042729.01.T01:CDS | 50.0% |
| GGCATGCAGTGATAGCCTGT+AGG | - | Chr8:13285432-13285451 | None:intergenic | 55.0% | |
| !! | AAGGCTCGGTTTGGGGGAAG+AGG | + | Chr8:13285927-13285946 | MsG0880042729.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 13285324 | 13285953 | 13285324 | ID=MsG0880042729.01;Name=MsG0880042729.01 |
| Chr8 | mRNA | 13285324 | 13285953 | 13285324 | ID=MsG0880042729.01.T01;Parent=MsG0880042729.01;Name=MsG0880042729.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|209 |
| Chr8 | exon | 13285324 | 13285953 | 13285324 | ID=MsG0880042729.01.T01:exon:36779;Parent=MsG0880042729.01.T01 |
| Chr8 | CDS | 13285324 | 13285953 | 13285324 | ID=MsG0880042729.01.T01:cds;Parent=MsG0880042729.01.T01 |
| Gene Sequence |
| Protein sequence |
