Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043017.01.T01 | XP_013444642.1 | 96.765 | 371 | 12 | 0 | 50 | 420 | 1 | 371 | 0 | 734 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047831.01 | MsG0880043017.01 | 0.842128 | 3.123962e-58 | 4.690614e-55 |
| MsG0080047967.01 | MsG0880043017.01 | -0.833545 | 5.015967e-56 | 5.795256e-53 |
| MsG0080048036.01 | MsG0880043017.01 | 0.864658 | 1.038118e-64 | 3.285267e-61 |
| MsG0080048059.01 | MsG0880043017.01 | -0.853160 | 2.870280e-61 | 6.143586e-58 |
| MsG0080048301.01 | MsG0880043017.01 | 0.826345 | 2.855252e-54 | 2.670645e-51 |
| MsG0080048347.01 | MsG0880043017.01 | 0.827380 | 1.615284e-54 | 1.557007e-51 |
| MsG0080048352.01 | MsG0880043017.01 | 0.823110 | 1.651966e-53 | 1.409373e-50 |
| MsG0080048486.01 | MsG0880043017.01 | 0.827219 | 1.765476e-54 | 1.693562e-51 |
| MsG0080048487.01 | MsG0880043017.01 | 0.826786 | 2.240427e-54 | 2.122071e-51 |
| MsG0080048488.01 | MsG0880043017.01 | 0.836037 | 1.183047e-56 | 1.472942e-53 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043017.01.T01 | MTR_8g027745 | 96.765 | 371 | 12 | 0 | 50 | 420 | 1 | 371 | 0.0 | 734 |
| MsG0880043017.01.T01 | MTR_7g117350 | 55.968 | 377 | 136 | 12 | 50 | 411 | 1 | 362 | 1.86e-130 | 380 |
| MsG0880043017.01.T01 | MTR_7g117350 | 54.805 | 385 | 136 | 13 | 50 | 411 | 1 | 370 | 8.44e-128 | 374 |
| MsG0880043017.01.T01 | MTR_3g091200 | 42.210 | 353 | 189 | 9 | 66 | 408 | 29 | 376 | 6.01e-84 | 262 |
| MsG0880043017.01.T01 | MTR_8g099515 | 39.722 | 360 | 178 | 13 | 75 | 408 | 36 | 382 | 3.68e-60 | 201 |
| MsG0880043017.01.T01 | MTR_8g099495 | 34.688 | 369 | 200 | 11 | 75 | 411 | 36 | 395 | 1.07e-49 | 174 |
| MsG0880043017.01.T01 | MTR_5g009190 | 35.000 | 320 | 191 | 10 | 95 | 408 | 31 | 339 | 7.50e-48 | 167 |
| MsG0880043017.01.T01 | MTR_5g009200 | 33.613 | 357 | 202 | 13 | 79 | 408 | 47 | 395 | 5.47e-47 | 166 |
| MsG0880043017.01.T01 | MTR_5g009210 | 33.062 | 369 | 207 | 16 | 70 | 408 | 2 | 360 | 1.78e-39 | 145 |
| MsG0880043017.01.T01 | MTR_3g012550 | 46.000 | 150 | 66 | 2 | 276 | 415 | 16 | 160 | 6.07e-38 | 135 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043017.01.T01 | AT2G46495 | 46.742 | 353 | 171 | 9 | 68 | 411 | 24 | 368 | 3.71e-94 | 288 |
| MsG0880043017.01.T01 | AT2G25410 | 43.988 | 341 | 186 | 5 | 75 | 410 | 34 | 374 | 4.95e-90 | 277 |
| MsG0880043017.01.T01 | AT2G46495 | 45.299 | 351 | 147 | 10 | 70 | 411 | 26 | 340 | 2.34e-85 | 264 |
| MsG0880043017.01.T01 | AT5G53110 | 39.489 | 352 | 188 | 10 | 73 | 408 | 33 | 375 | 1.18e-75 | 241 |
| MsG0880043017.01.T01 | AT2G46494 | 42.900 | 331 | 174 | 8 | 85 | 409 | 41 | 362 | 3.51e-75 | 239 |
| MsG0880043017.01.T01 | AT1G28040 | 42.623 | 305 | 152 | 9 | 117 | 407 | 2 | 297 | 4.59e-63 | 205 |
| MsG0880043017.01.T01 | AT5G36001 | 37.571 | 354 | 156 | 10 | 70 | 420 | 30 | 321 | 1.97e-59 | 197 |
| MsG0880043017.01.T01 | AT5G07040 | 47.586 | 145 | 73 | 2 | 275 | 419 | 9 | 150 | 2.82e-38 | 136 |
| MsG0880043017.01.T01 | AT5G36001 | 40.976 | 205 | 100 | 7 | 70 | 271 | 30 | 216 | 8.52e-32 | 120 |
| MsG0880043017.01.T01 | AT2G46493 | 34.597 | 211 | 107 | 5 | 202 | 409 | 2 | 184 | 2.75e-31 | 118 |
Find 88 sgRNAs with CRISPR-Local
Find 228 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACCAGGATAGCCACAATA+AGG | 0.435316 | 8:-17743222 | None:intergenic |
| AACCCATCTTCAAGAAGGGC+CGG | 0.477868 | 8:+17742413 | MsG0880043017.01.T01:CDS |
| AACCGTTCTGGTCTTGCAAT+TGG | 0.365731 | 8:-17743585 | None:intergenic |
| AAGTACCAAGCTATGAGGGC+AGG | 0.567054 | 8:+17742332 | MsG0880043017.01.T01:CDS |
| AATCATTCAAGCATTACTAG+AGG | 0.645917 | 8:-17745721 | None:intergenic |
| ACATAGAATTCTTCAGAGTA+AGG | 0.498143 | 8:-17743288 | None:intergenic |
| ACATTAAGAGGAGAAGGAGC+AGG | 0.559825 | 8:-17745696 | None:intergenic |
| ACCCATCTTCAAGAAGGGCC+GGG | 0.496704 | 8:+17742414 | MsG0880043017.01.T01:CDS |
| ACCCCGGCCCTTCTTGAAGA+TGG | 0.391942 | 8:-17742416 | None:intergenic |
| ACGTTTGGGAAGACAATCAT+CGG | 0.534356 | 8:-17743355 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACGTTGCACTCTTTAAATT+TGG | - | Chr8:17744258-17744277 | None:intergenic | 30.0% | |
| AATGGTAAACAAATTGGATG+GGG | - | Chr8:17741886-17741905 | None:intergenic | 30.0% | |
| ACATAGAATTCTTCAGAGTA+AGG | - | Chr8:17743291-17743310 | None:intergenic | 30.0% | |
| AGAATGGTAAACAAATTGGA+TGG | - | Chr8:17741888-17741907 | None:intergenic | 30.0% | |
| AGAGTTACTAAGACAATCAA+TGG | - | Chr8:17743484-17743503 | None:intergenic | 30.0% | |
| ATTATACTTCACTAATGGCA+CGG | - | Chr8:17744451-17744470 | None:intergenic | 30.0% | |
| CATGTGGGAAATTAATGAAT+AGG | - | Chr8:17742528-17742547 | None:intergenic | 30.0% | |
| CGACTAAAAGAAGCAATAAA+AGG | - | Chr8:17743408-17743427 | None:intergenic | 30.0% | |
| GAAATAACAAAAGGAAGAGT+TGG | - | Chr8:17744884-17744903 | None:intergenic | 30.0% | |
| GAAATTATTTGTGAGCAATG+AGG | + | Chr8:17744760-17744779 | MsG0880043017.01.T01:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 17741633 | 17745737 | 17741633 | ID=MsG0880043017.01;Name=MsG0880043017.01 |
| Chr8 | mRNA | 17741633 | 17745737 | 17741633 | ID=MsG0880043017.01.T01;Parent=MsG0880043017.01;Name=MsG0880043017.01.T01;_AED=0.33;_eAED=0.38;_QI=0|0|0|0.75|0.33|0.25|4|0|420 |
| Chr8 | exon | 17741633 | 17741655 | 17741633 | ID=MsG0880043017.01.T01:exon:3449;Parent=MsG0880043017.01.T01 |
| Chr8 | exon | 17742318 | 17742436 | 17742318 | ID=MsG0880043017.01.T01:exon:3450;Parent=MsG0880043017.01.T01 |
| Chr8 | exon | 17743088 | 17743756 | 17743088 | ID=MsG0880043017.01.T01:exon:3451;Parent=MsG0880043017.01.T01 |
| Chr8 | exon | 17745286 | 17745737 | 17745286 | ID=MsG0880043017.01.T01:exon:3452;Parent=MsG0880043017.01.T01 |
| Chr8 | CDS | 17741633 | 17741655 | 17741633 | ID=MsG0880043017.01.T01:cds;Parent=MsG0880043017.01.T01 |
| Chr8 | CDS | 17742318 | 17742436 | 17742318 | ID=MsG0880043017.01.T01:cds;Parent=MsG0880043017.01.T01 |
| Chr8 | CDS | 17743088 | 17743756 | 17743088 | ID=MsG0880043017.01.T01:cds;Parent=MsG0880043017.01.T01 |
| Chr8 | CDS | 17745286 | 17745737 | 17745286 | ID=MsG0880043017.01.T01:cds;Parent=MsG0880043017.01.T01 |
| Gene Sequence |
>MsG0880043017.01.T01 ATGTCCCTGACTCAATTACTGAACAGGTCTTCATGTGAAGTACCAAGCTATGAGGGCAGGACCTGGTGGAGAAGTTGTTTCCATCAGAATCTCTCAGCAGCTGCTGTGACTCATCTCAAACCCATCTTCAAGAAGGGCCGGGAAGTAATGGACATCTTGAAGGTCCTTTTTTACTTAATCTTTCCTGTCGCATACGCCATAAACGATTGCCCTTTTTCTTTATGTGGCAACAATAGTTTCCTAATTCGTTTCCCCTTTCAGTTAGGTGAGCAATATCCTTATTGTGGCTATCCTGGTTTCAACTTAAATTGTACCAATGATAGCAAAACAATTCTAAAACTTCCTTACTCTGAAGAATTCTATGTACGCAGCATAAACTATCTCACGCAACAGATACTACTCTATGATCCCGATGATTGTCTTCCCAAACGTCTTCTAAGCCTCAATTTTTCAAATTCTCCTTTTATTGCTTCTTTTAGTCGTGACTACACCTTCTTAGGCTGTTCATCTCAGAATATAGGGTCACAATTCATTCCCATTGATTGTCTTAGTAACTCTACATATTTTGTATCAGCCATTCCTTCAGTTAGCTTTGTCAATTCTCTGTCTGGTTGCTCTGTTATAAAAAACCTTTCAGTTCCAATTGCAAGACCAGAACGGTTTCAAGAAAATCTAAGAGATGACCTTAGTGCAGATCTTCAATTAACATGGGATAAACCTGACTGCAGTTATTGTGAATCGCATCAGCTAATGTGTGGATTTGAAAGCATTAACAGCAATCAAGTTGTCTGTTTCTCTGATTACCAACCAGGTACATCGCGACAAGGACTTAAAATTTTCAGAATCATAGCACTATGCGTCACTGGACCAGCTCTGATCATTGTTATTCTAATGGCAAGTTGTGTGTGCTACAATGATAGAATAGGCAACATTACCAGAAGTGCTGCTTCTCGGTCAGCACCAGCGGCAATATCACCGCAACCTGAAGCCACAGTAATTGCAGGTTTAGATGAATCCACCATTGAATCTTATGAGAAAGTAATTCTTGGGGAGAGTCGACGTGTTCCTGGACCAAACGACGGAAGTTGTTGGATATGCCTGGCAGAATACAACAGTAAAGAGACAGTAAGATGCATTCCTGAATGCAAACATTGTTTCCATGCTGATTGCATCGATGAGTGGCTTCGTATGAATGTTACATGCCCTGTTTGTCGTAACTCTCCTGCTCCTTCTCCTCTTAATGTTGCCTCTAGTAATGCTTGA |
| Protein sequence |
>MsG0880043017.01.T01 MSLTQLLNRSSCEVPSYEGRTWWRSCFHQNLSAAAVTHLKPIFKKGREVMDILKVLFYLIFPVAYAINDCPFSLCGNNSFLIRFPFQLGEQYPYCGYPGFNLNCTNDSKTILKLPYSEEFYVRSINYLTQQILLYDPDDCLPKRLLSLNFSNSPFIASFSRDYTFLGCSSQNIGSQFIPIDCLSNSTYFVSAIPSVSFVNSLSGCSVIKNLSVPIARPERFQENLRDDLSADLQLTWDKPDCSYCESHQLMCGFESINSNQVVCFSDYQPGTSRQGLKIFRIIALCVTGPALIIVILMASCVCYNDRIGNITRSAASRSAPAAISPQPEATVIAGLDESTIESYEKVILGESRRVPGPNDGSCWICLAEYNSKETVRCIPECKHCFHADCIDEWLRMNVTCPVCRNSPAPSPLNVASSNA* |
