AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043294.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043294.01.T01 MTR_8g432670 69.947 376 38 6 1 304 81 453 1.27e-162 463
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043294.01.T01 AT4G00752 26.975 367 169 13 3 305 91 422 1.99e-18 85.9
MsG0880043294.01.T01 AT4G23040 30.114 176 60 5 177 304 318 478 5.51e-13 69.7
MsG0880043294.01.T01 AT4G11740 32.558 172 70 4 177 304 348 517 3.16e-11 64.7

Find 62 sgRNAs with CRISPR-Local

Find 225 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGATTGAAACTGCGCTTTA+TGG 0.169001 8:+22144325 MsG0880043294.01.T01:CDS
TATCATTTCACTGTCTTTAC+AGG 0.210054 8:+22143354 MsG0880043294.01.T01:CDS
TTGAGGATGTCACGCTTTAT+AGG 0.262004 8:+22146258 MsG0880043294.01.T01:intron
TGAACGAGAAGATGATGATT+TGG 0.317721 8:+22143327 MsG0880043294.01.T01:CDS
TTCTCTGATCTCTTTCTTTG+AGG 0.319805 8:-22143157 None:intergenic
ACTGGATGAACCCTGCCTTT+TGG 0.331875 8:-22144425 None:intergenic
CTTCAAAACTGACAAGCTTC+AGG 0.348852 8:+22145313 MsG0880043294.01.T01:CDS
TCGCCTAAGGGTGGTTCATT+TGG 0.363564 8:-22145230 None:intergenic
TTCTCGTTCAAATGACTTTG+TGG 0.369903 8:-22143314 None:intergenic
CAATCCAGGTTGATACTGAT+AGG 0.420651 8:-22142978 None:intergenic
TCAGTTGATAAGACAACAAC+AGG 0.428917 8:+22144474 MsG0880043294.01.T01:CDS
CAACCTGGATTGGGCTATGC+AGG 0.430431 8:+22142989 MsG0880043294.01.T01:CDS
TAATAACTGAGATGCTGAAC+TGG 0.436931 8:-22144443 None:intergenic
TACTATTGTTGTTCGAATGC+CGG 0.439751 8:+22145265 MsG0880043294.01.T01:CDS
AGAGATCTATGTAACGGGTT+TGG 0.445773 8:+22142860 MsG0880043294.01.T01:CDS
TATCAGTATCAACCTGGATT+GGG 0.450112 8:+22142980 MsG0880043294.01.T01:CDS
AATGCTTCATTGAGCTCGTC+AGG 0.450397 8:-22144301 None:intergenic
CTATCAGTATCAACCTGGAT+TGG 0.452638 8:+22142979 MsG0880043294.01.T01:CDS
AACTTAGAGATCTATGTAAC+GGG 0.459209 8:+22142855 MsG0880043294.01.T01:CDS
TACGGAGCTTCGAGCGAAAG+AGG 0.459869 8:+22143485 MsG0880043294.01.T01:CDS
TCTGAATGATGTTGTGGGTC+AGG 0.465319 8:-22144388 None:intergenic
AATTTAGACTGCAGAGAAAG+AGG 0.466485 8:+22143456 MsG0880043294.01.T01:intron
TTGCAGGAACTGGAGAAAAC+AGG 0.468610 8:+22145185 MsG0880043294.01.T01:intron
GTTCTCCACAAGGGGAGGCA+AGG 0.512550 8:+22142918 MsG0880043294.01.T01:CDS
TACTACTTGACTCAAACTAT+AGG 0.513851 8:+22142828 MsG0880043294.01.T01:CDS
AATTCCCTTGCCTCCCCTTG+TGG 0.513986 8:-22142923 None:intergenic
AAATGCTGATCCCAAAAGGC+AGG 0.520325 8:+22144414 MsG0880043294.01.T01:CDS
CGTTCACAACGACCACCGTC+CGG 0.525938 8:-22145284 None:intergenic
TTCTCCACAAGGGGAGGCAA+GGG 0.527047 8:+22142919 MsG0880043294.01.T01:CDS
CTTCCAAATGAACCACCCTT+AGG 0.529218 8:+22145227 MsG0880043294.01.T01:CDS
ATGAGATGATAAATTTCCAT+TGG 0.530591 8:-22143020 None:intergenic
GCACCTGCATAGCCCAATCC+AGG 0.530699 8:-22142992 None:intergenic
TGCTCATGCTTTATTAGCCG+AGG 0.531346 8:+22143524 MsG0880043294.01.T01:CDS
TGTTGTGGGTCAGGCAAAGA+TGG 0.536551 8:-22144379 None:intergenic
TTGTCTTATCAACTGAGTCT+CGG 0.537380 8:-22144467 None:intergenic
ATTCCACTCGCACATTGTGC+AGG 0.543374 8:-22146295 None:intergenic
GAGGCAAGGGAATTTCCTAC+AGG 0.546922 8:+22142932 MsG0880043294.01.T01:CDS
GTTAGTAGTTCTCTCTCCCT+CGG 0.562193 8:-22143541 None:intergenic
AATGGAAATTTATCATCTCA+TGG 0.563505 8:+22143022 MsG0880043294.01.T01:CDS
CAGAGAAAGAGGAAGCAATA+CGG 0.564131 8:+22143467 MsG0880043294.01.T01:intron
TTTGAGTCAAGTAGTAGTGA+AGG 0.568566 8:-22142821 None:intergenic
GAACTTAGAGATCTATGTAA+CGG 0.570755 8:+22142854 MsG0880043294.01.T01:CDS
CCACCACGTGGTTCTCCACA+AGG 0.577108 8:+22142908 MsG0880043294.01.T01:CDS
ATTGTTGTTCGAATGCCGGA+CGG 0.596739 8:+22145269 MsG0880043294.01.T01:CDS
AATCCAGGTTGATACTGATA+GGG 0.600870 8:-22142977 None:intergenic
AAAGAGGAAAGTAAAGTACT+TGG 0.601216 8:+22143501 MsG0880043294.01.T01:CDS
ACCACGTGGTTCTCCACAAG+GGG 0.608202 8:+22142910 MsG0880043294.01.T01:CDS
TCACCCTATCAGTATCAACC+TGG 0.609062 8:+22142974 MsG0880043294.01.T01:CDS
GCTCATGCTTTATTAGCCGA+GGG 0.610195 8:+22143525 MsG0880043294.01.T01:CDS
ACGTGGTTCTCCACAAGGGG+AGG 0.620464 8:+22142913 MsG0880043294.01.T01:CDS
CTTCTAGCAGCATTGAACAA+AGG 0.626567 8:-22142791 None:intergenic
GTTGTTCGAATGCCGGACGG+TGG 0.634608 8:+22145272 MsG0880043294.01.T01:CDS
CACCACGTGGTTCTCCACAA+GGG 0.639472 8:+22142909 MsG0880043294.01.T01:CDS
CACTAACAGAAGCAAACTAG+AGG 0.646420 8:+22143563 MsG0880043294.01.T01:CDS
AATGCTGATCCCAAAAGGCA+GGG 0.658565 8:+22144415 MsG0880043294.01.T01:CDS
TATGCAGGTGCTGATACCAA+TGG 0.672316 8:+22143004 MsG0880043294.01.T01:CDS
TGTGCACTATTGATCCCTGT+AGG 0.694302 8:-22142947 None:intergenic
AGGCAAGGGAATTTCCTACA+GGG 0.721531 8:+22142933 MsG0880043294.01.T01:CDS
TCAAGTAGTACACCACCACG+TGG 0.740632 8:+22142896 MsG0880043294.01.T01:CDS
TGACCTGCACAATGTGCGAG+TGG 0.741495 8:+22146292 MsG0880043294.01.T01:CDS
CCTTGTGGAGAACCACGTGG+TGG 0.741616 8:-22142908 None:intergenic
TCCCCTTGTGGAGAACCACG+TGG 0.753351 8:-22142911 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAGTAAAACAAAATATTTT+AGG - Chr8:22143776-22143795 None:intergenic 10.0%
!! CACAAAAATAAAAAAATATG+TGG - Chr8:22145654-22145673 None:intergenic 15.0%
!!! TATCACATCATAATTTTTTT+GGG + Chr8:22143735-22143754 MsG0880043294.01.T01:intron 15.0%
!!! TTATCACATCATAATTTTTT+TGG + Chr8:22143734-22143753 MsG0880043294.01.T01:intron 15.0%
!! ATAAAAAAGTCAAAAGAATC+AGG - Chr8:22145100-22145119 None:intergenic 20.0%
!! ATGATGTGATAAAAAAATCA+AGG - Chr8:22143726-22143745 None:intergenic 20.0%
!! ATGTCATAGTAAAATAACAA+AGG - Chr8:22146132-22146151 None:intergenic 20.0%
!! GAAATAGAAGTAACAAAATA+TGG - Chr8:22143231-22143250 None:intergenic 20.0%
!! GAATATTCATATTAAAGTGT+TGG + Chr8:22144879-22144898 MsG0880043294.01.T01:intron 20.0%
!! TCAAATGTATTAACTTAAGT+CGG + Chr8:22143987-22144006 MsG0880043294.01.T01:intron 20.0%
!! TGAATGCTGAAATAATATAT+TGG - Chr8:22143279-22143298 None:intergenic 20.0%
!! TGAGTCATATACAAATTAAA+AGG - Chr8:22143390-22143409 None:intergenic 20.0%
!! TTTAATATGAATATTCATGC+AGG - Chr8:22144875-22144894 None:intergenic 20.0%
!!! AAGGTTTTAAATTACAATTG+CGG - Chr8:22145720-22145739 None:intergenic 20.0%
!!! ATCAAGGATTTTTATGTATT+TGG + Chr8:22145686-22145705 MsG0880043294.01.T01:intron 20.0%
!!! TTTGATTTCATTGACATTTA+TGG + Chr8:22146038-22146057 MsG0880043294.01.T01:intron 20.0%
!!! TTTGTTATTTTACTATGACA+TGG + Chr8:22146131-22146150 MsG0880043294.01.T01:intron 20.0%
! AAATTCTATTAAGCCATTTC+AGG + Chr8:22143887-22143906 MsG0880043294.01.T01:intron 25.0%
! AATGGAAATTTATCATCTCA+TGG + Chr8:22143022-22143041 MsG0880043294.01.T01:CDS 25.0%
! ACTTGATAAAACAGTTTCAT+GGG + Chr8:22144051-22144070 MsG0880043294.01.T01:intron 25.0%
! ATGAATTAGAAATTACTCAG+AGG - Chr8:22144824-22144843 None:intergenic 25.0%
! ATGAGATGATAAATTTCCAT+TGG - Chr8:22143023-22143042 None:intergenic 25.0%
! ATGCTGAAATAATATATTGG+AGG - Chr8:22143276-22143295 None:intergenic 25.0%
! CAATCCATAAATCAATTGAT+AGG - Chr8:22146107-22146126 None:intergenic 25.0%
! TTGATCATACACTTATCAAA+TGG - Chr8:22145515-22145534 None:intergenic 25.0%
!! ATCTAAAGCTACATTTCAAT+TGG - Chr8:22144158-22144177 None:intergenic 25.0%
!! ATGTGTATTTTACATTACTG+CGG + Chr8:22145960-22145979 MsG0880043294.01.T01:intron 25.0%
!! TCATCCTATCAATTGATTTA+TGG + Chr8:22146100-22146119 MsG0880043294.01.T01:intron 25.0%
!! TGTGATTTTATGCAATATCA+AGG + Chr8:22145670-22145689 MsG0880043294.01.T01:intron 25.0%
!!! CTTTTTTATATTCCTTTGAC+AGG + Chr8:22145111-22145130 MsG0880043294.01.T01:intron 25.0%
AAACAAAATTGTCCTGTCAA+AGG - Chr8:22145126-22145145 None:intergenic 30.0%
AAACTAAGAATCAGAAACAC+TGG - Chr8:22143425-22143444 None:intergenic 30.0%
AAATCAAGGTTTGTGAACTA+CGG - Chr8:22143712-22143731 None:intergenic 30.0%
AACCAACTAAGAAATCAACT+AGG + Chr8:22145795-22145814 MsG0880043294.01.T01:intron 30.0%
AACTAAGAATCAGAAACACT+GGG - Chr8:22143424-22143443 None:intergenic 30.0%
AACTTAGAGATCTATGTAAC+GGG + Chr8:22142855-22142874 MsG0880043294.01.T01:CDS 30.0%
AATTAATTGCTAACGAAGCA+AGG - Chr8:22145739-22145758 None:intergenic 30.0%
AATTTATCATCTCATGGTAG+AGG + Chr8:22143028-22143047 MsG0880043294.01.T01:intron 30.0%
ACAAAAATACGCTTGAAGAA+CGG + Chr8:22144670-22144689 MsG0880043294.01.T01:intron 30.0%
CACTTGATAAAACAGTTTCA+TGG + Chr8:22144050-22144069 MsG0880043294.01.T01:intron 30.0%
GAACTTAGAGATCTATGTAA+CGG + Chr8:22142854-22142873 MsG0880043294.01.T01:CDS 30.0%
GTATGATCTATATCCATGAA+AGG + Chr8:22144521-22144540 MsG0880043294.01.T01:intron 30.0%
TAACATGATTACCAAGTGAA+TGG - Chr8:22145034-22145053 None:intergenic 30.0%
TACATATGCAGTTATGAAGT+AGG + Chr8:22145932-22145951 MsG0880043294.01.T01:intron 30.0%
TACTACTTGACTCAAACTAT+AGG + Chr8:22142828-22142847 MsG0880043294.01.T01:CDS 30.0%
TATCATTTCACTGTCTTTAC+AGG + Chr8:22143354-22143373 MsG0880043294.01.T01:CDS 30.0%
TGAAATCAAAAGAAGCTACA+AGG - Chr8:22146028-22146047 None:intergenic 30.0%
TGATAAGTGTATGATCAAGT+TGG + Chr8:22145517-22145536 MsG0880043294.01.T01:intron 30.0%
! AAAGAGGAAAGTAAAGTACT+TGG + Chr8:22143501-22143520 MsG0880043294.01.T01:CDS 30.0%
! ATTATTTTCATGAGTTCGTC+TGG + Chr8:22144842-22144861 MsG0880043294.01.T01:intron 30.0%
! GCTTTTATTACCATCTTTTG+AGG + Chr8:22146241-22146260 MsG0880043294.01.T01:intron 30.0%
! GTTTTACTAAAAAGCCCTTA+GGG + Chr8:22143786-22143805 MsG0880043294.01.T01:intron 30.0%
! TATCTTTTCCAATTGAAGTC+AGG + Chr8:22144124-22144143 MsG0880043294.01.T01:intron 30.0%
! TGGAGGAAAAAACTTTAGAA+AGG - Chr8:22143259-22143278 None:intergenic 30.0%
! TGTTTTACTAAAAAGCCCTT+AGG + Chr8:22143785-22143804 MsG0880043294.01.T01:intron 30.0%
! TTATTTTCATGAGTTCGTCT+GGG + Chr8:22144843-22144862 MsG0880043294.01.T01:intron 30.0%
! TTTTCTCTGAATGATGTTGT+GGG - Chr8:22144396-22144415 None:intergenic 30.0%
! TTTTTCTCTGAATGATGTTG+TGG - Chr8:22144397-22144416 None:intergenic 30.0%
!! ATTAAGCCATTTCAGGTTAT+GGG + Chr8:22143894-22143913 MsG0880043294.01.T01:intron 30.0%
!! GTGGTCGTTTTCATAAATAA+TGG + Chr8:22146208-22146227 MsG0880043294.01.T01:intron 30.0%
!! TATTAAGCCATTTCAGGTTA+TGG + Chr8:22143893-22143912 MsG0880043294.01.T01:intron 30.0%
!!! CCTTATTTTGCAATATAGTG+GGG + Chr8:22144268-22144287 MsG0880043294.01.T01:intron 30.0%
!!! GCCTTATTTTGCAATATAGT+GGG + Chr8:22144267-22144286 MsG0880043294.01.T01:intron 30.0%
!!! GCTATGTATTTTAATCAACC+TGG - Chr8:22143867-22143886 None:intergenic 30.0%
!!! GTATTTTAATCAACCTGGTA+AGG - Chr8:22143862-22143881 None:intergenic 30.0%
!!! TGCCTTATTTTGCAATATAG+TGG + Chr8:22144266-22144285 MsG0880043294.01.T01:intron 30.0%
AAATGTGTTGCGTGATAAGA+AGG - Chr8:22144021-22144040 None:intergenic 35.0%
AACAGTGTTTACCTATTTGC+AGG + Chr8:22145169-22145188 MsG0880043294.01.T01:intron 35.0%
AAGCACTTGATTGAGCTAAA+GGG - Chr8:22143086-22143105 None:intergenic 35.0%
AATTTAGACTGCAGAGAAAG+AGG + Chr8:22143456-22143475 MsG0880043294.01.T01:intron 35.0%
ACCATACAAGGATGATTCAA+TGG - Chr8:22144775-22144794 None:intergenic 35.0%
ACCATTGAATCATCCTTGTA+TGG + Chr8:22144771-22144790 MsG0880043294.01.T01:intron 35.0%
AGAAAAATGCTGATCCCAAA+AGG + Chr8:22144410-22144429 MsG0880043294.01.T01:CDS 35.0%
AGAAACACTCACAACTCTAT+AGG - Chr8:22146081-22146100 None:intergenic 35.0%
AGAACCACATATAACCCTAA+GGG - Chr8:22143803-22143822 None:intergenic 35.0%
AGCAGAGACATACAATGAAT+TGG + Chr8:22143641-22143660 MsG0880043294.01.T01:intron 35.0%
AGGACAAGGTTGAACTTTAA+AGG - Chr8:22145417-22145436 None:intergenic 35.0%
AGTACAAAAGGATGTTTGCT+AGG - Chr8:22145384-22145403 None:intergenic 35.0%
CAGGTTATGTTATCCTTTCA+TGG - Chr8:22144537-22144556 None:intergenic 35.0%
CCCCACTATATTGCAAAATA+AGG - Chr8:22144271-22144290 None:intergenic 35.0%
CTCATGGTAGAGGATATTAT+GGG + Chr8:22143038-22143057 MsG0880043294.01.T01:intron 35.0%
CTTGAAGAACGGTAATACAT+TGG + Chr8:22144681-22144700 MsG0880043294.01.T01:intron 35.0%
GCAGAGACATACAATGAATT+GGG + Chr8:22143642-22143661 MsG0880043294.01.T01:intron 35.0%
GCTACATTTCAATTGGATGA+TGG - Chr8:22144151-22144170 None:intergenic 35.0%
GGACAAGGTTGAACTTTAAA+GGG - Chr8:22145416-22145435 None:intergenic 35.0%
GGTAATCATGTTAACACTTG+TGG + Chr8:22145041-22145060 MsG0880043294.01.T01:intron 35.0%
TAAAGTTCAACCTTGTCCTA+CGG + Chr8:22145418-22145437 MsG0880043294.01.T01:intron 35.0%
TAATAACTGAGATGCTGAAC+TGG - Chr8:22144446-22144465 None:intergenic 35.0%
TAATCAACCTGGTAAGGAAT+GGG - Chr8:22143856-22143875 None:intergenic 35.0%
TAATTGCGACAGCAACATAA+TGG + Chr8:22145578-22145597 MsG0880043294.01.T01:intron 35.0%
TACTGATGCTTTCTTTAGCT+GGG + Chr8:22145840-22145859 MsG0880043294.01.T01:intron 35.0%
TATCAGTATCAACCTGGATT+GGG + Chr8:22142980-22142999 MsG0880043294.01.T01:CDS 35.0%
TCTCATGGTAGAGGATATTA+TGG + Chr8:22143037-22143056 MsG0880043294.01.T01:intron 35.0%
TGAACGAGAAGATGATGATT+TGG + Chr8:22143327-22143346 MsG0880043294.01.T01:CDS 35.0%
TTAATCAACCTGGTAAGGAA+TGG - Chr8:22143857-22143876 None:intergenic 35.0%
TTCTCGTTCAAATGACTTTG+TGG - Chr8:22143317-22143336 None:intergenic 35.0%
TTCTCTGATCTCTTTCTTTG+AGG - Chr8:22143160-22143179 None:intergenic 35.0%
TTGTCTTATCAACTGAGTCT+CGG - Chr8:22144470-22144489 None:intergenic 35.0%
TTGTTGCTAAAGATGTTCAG+TGG + Chr8:22146166-22146185 MsG0880043294.01.T01:intron 35.0%
TTTGAGTCAAGTAGTAGTGA+AGG - Chr8:22142824-22142843 None:intergenic 35.0%
! AAGTTGACGTGTCTTTTGTA+TGG + Chr8:22145072-22145091 MsG0880043294.01.T01:intron 35.0%
! AATTGGGTGGTTCTATATGA+AGG + Chr8:22143658-22143677 MsG0880043294.01.T01:intron 35.0%
! CAGCAACATAATGGTTTTTG+TGG + Chr8:22145587-22145606 MsG0880043294.01.T01:intron 35.0%
! GACAAGGTTGAACTTTAAAG+GGG - Chr8:22145415-22145434 None:intergenic 35.0%
! GTCCTAGTTGATTTCTTAGT+TGG - Chr8:22145800-22145819 None:intergenic 35.0%
! TACTATTGTTGTTCGAATGC+CGG + Chr8:22145265-22145284 MsG0880043294.01.T01:CDS 35.0%
! TGTTTGCTAGGAAGAATACA+AGG - Chr8:22145372-22145391 None:intergenic 35.0%
! TTTTACATTACTGCGGTAGT+TGG + Chr8:22145967-22145986 MsG0880043294.01.T01:intron 35.0%
!! AATCCAGGTTGATACTGATA+GGG - Chr8:22142980-22142999 None:intergenic 35.0%
!! CAGGACAATTTTGTTTCCAT+TGG + Chr8:22145130-22145149 MsG0880043294.01.T01:intron 35.0%
!! CAGTACTGTTCTAAAACCAA+TGG - Chr8:22145149-22145168 None:intergenic 35.0%
!! TATTTTGCAATATAGTGGGG+TGG + Chr8:22144271-22144290 MsG0880043294.01.T01:intron 35.0%
!! TCAGTTGATAAGACAACAAC+AGG + Chr8:22144474-22144493 MsG0880043294.01.T01:CDS 35.0%
!!! AATTCTAGTTTTCAGACCGT+AGG - Chr8:22145437-22145456 None:intergenic 35.0%
!!! AATTGCTTTTTCATCGCCTA+AGG - Chr8:22145246-22145265 None:intergenic 35.0%
!!! ATTGCTTTTTCATCGCCTAA+GGG - Chr8:22145245-22145264 None:intergenic 35.0%
AAAGCCCTTAGGGTTATATG+TGG + Chr8:22143796-22143815 MsG0880043294.01.T01:intron 40.0%
AACAGAAGCAAACTAGAGGT+GGG + Chr8:22143567-22143586 MsG0880043294.01.T01:intron 40.0%
AAGAATCAGAAACACTGGGA+AGG - Chr8:22143420-22143439 None:intergenic 40.0%
AAGGATGATTCAATGGTCAG+CGG - Chr8:22144768-22144787 None:intergenic 40.0%
ACCTGTCAACATCTTTATGC+AGG + Chr8:22144552-22144571 MsG0880043294.01.T01:intron 40.0%
ACTTAAAACCATGCCACCAA+GGG - Chr8:22145544-22145563 None:intergenic 40.0%
ATAGATCATACAGCCATTGC+AGG - Chr8:22144512-22144531 None:intergenic 40.0%
ATGAGAGAGATGAAACAGGA+AGG + Chr8:22145863-22145882 MsG0880043294.01.T01:intron 40.0%
ATGATGGTCCTGACTTCAAT+TGG - Chr8:22144135-22144154 None:intergenic 40.0%
CACTAACAGAAGCAAACTAG+AGG + Chr8:22143563-22143582 MsG0880043294.01.T01:CDS 40.0%
CAGAACCACATATAACCCTA+AGG - Chr8:22143804-22143823 None:intergenic 40.0%
CAGAGAAAGAGGAAGCAATA+CGG + Chr8:22143467-22143486 MsG0880043294.01.T01:intron 40.0%
CTACGATACTTGACCATACA+AGG - Chr8:22144787-22144806 None:intergenic 40.0%
CTACTGATGCTTTCTTTAGC+TGG + Chr8:22145839-22145858 MsG0880043294.01.T01:intron 40.0%
CTATCAGTATCAACCTGGAT+TGG + Chr8:22142979-22142998 MsG0880043294.01.T01:CDS 40.0%
CTGATTGAAACTGCGCTTTA+TGG + Chr8:22144325-22144344 MsG0880043294.01.T01:CDS 40.0%
CTTCAAAACTGACAAGCTTC+AGG + Chr8:22145313-22145332 MsG0880043294.01.T01:CDS 40.0%
GAGACATACAATGAATTGGG+TGG + Chr8:22143645-22143664 MsG0880043294.01.T01:intron 40.0%
GGTTTGTGAACTACGGAAAA+TGG - Chr8:22143705-22143724 None:intergenic 40.0%
GTTTACCTATTTGCAGGAAC+TGG + Chr8:22145175-22145194 MsG0880043294.01.T01:intron 40.0%
TAACAGAAGCAAACTAGAGG+TGG + Chr8:22143566-22143585 MsG0880043294.01.T01:intron 40.0%
TACGGAAAATGGAATGACCA+TGG - Chr8:22143694-22143713 None:intergenic 40.0%
TCCTGCATAAAGATGTTGAC+AGG - Chr8:22144556-22144575 None:intergenic 40.0%
TGACACATGCAAAAGACGAA+TGG - Chr8:22144952-22144971 None:intergenic 40.0%
TGACATTTATGGAGCAGTGA+AGG + Chr8:22146049-22146068 MsG0880043294.01.T01:intron 40.0%
TGGCATAATCTGTTGTCAAG+TGG + Chr8:22146189-22146208 MsG0880043294.01.T01:intron 40.0%
TGGTGCATGTTCAAAAGACA+AGG - Chr8:22145014-22145033 None:intergenic 40.0%
TTCATAGCTCAGCTAACAGT+CGG - Chr8:22144729-22144748 None:intergenic 40.0%
TTGAACATGCACCATTCACT+TGG + Chr8:22145020-22145039 MsG0880043294.01.T01:intron 40.0%
TTGAGGATGTCACGCTTTAT+AGG + Chr8:22146258-22146277 MsG0880043294.01.T01:intron 40.0%
TTGCTAAAGATGTTCAGTGG+TGG + Chr8:22146169-22146188 MsG0880043294.01.T01:intron 40.0%
TTTACACACGCATGACTCTT+CGG + Chr8:22143613-22143632 MsG0880043294.01.T01:intron 40.0%
TTTCTCCAGTTCCTGCAAAT+AGG - Chr8:22145183-22145202 None:intergenic 40.0%
! AGGTTGAACTTTAAAGGGGT+TGG - Chr8:22145411-22145430 None:intergenic 40.0%
! CTTCTAGCAGCATTGAACAA+AGG - Chr8:22142794-22142813 None:intergenic 40.0%
! GAAGCACTTGATTGAGCTAA+AGG - Chr8:22143087-22143106 None:intergenic 40.0%
! TGAACTTTAAAGGGGTTGGT+AGG - Chr8:22145407-22145426 None:intergenic 40.0%
! TTTTCTTCTGCTTGACGAGT+GGG - Chr8:22144647-22144666 None:intergenic 40.0%
! TTTTTCTTCTGCTTGACGAG+TGG - Chr8:22144648-22144667 None:intergenic 40.0%
!! AGAGATCTATGTAACGGGTT+TGG + Chr8:22142860-22142879 MsG0880043294.01.T01:CDS 40.0%
!! CAATCCAGGTTGATACTGAT+AGG - Chr8:22142981-22143000 None:intergenic 40.0%
AAATGCTGATCCCAAAAGGC+AGG + Chr8:22144414-22144433 MsG0880043294.01.T01:CDS 45.0%
AATGCTGATCCCAAAAGGCA+GGG + Chr8:22144415-22144434 MsG0880043294.01.T01:CDS 45.0%
AATGCTTCATTGAGCTCGTC+AGG - Chr8:22144304-22144323 None:intergenic 45.0%
ACGTGTTCATGAAGATGCAG+AGG + Chr8:22143107-22143126 MsG0880043294.01.T01:intron 45.0%
AGCGTGACATCCTCAAAAGA+TGG - Chr8:22146254-22146273 None:intergenic 45.0%
AGCTTTGCATCACACACTCT+TGG - Chr8:22144205-22144224 None:intergenic 45.0%
AGGCAAGGGAATTTCCTACA+GGG + Chr8:22142933-22142952 MsG0880043294.01.T01:CDS 45.0%
ATTTATGGAGCAGTGAAGGC+TGG + Chr8:22146053-22146072 MsG0880043294.01.T01:intron 45.0%
CAAATGGTTACCACAACTCC+TGG - Chr8:22145499-22145518 None:intergenic 45.0%
CACTTAAAACCATGCCACCA+AGG - Chr8:22145545-22145564 None:intergenic 45.0%
CGACAACCCATAACCTGAAA+TGG - Chr8:22143903-22143922 None:intergenic 45.0%
CTTCCAAATGAACCACCCTT+AGG + Chr8:22145227-22145246 MsG0880043294.01.T01:CDS 45.0%
GAATGGGCATGGACAAAAGT+AGG - Chr8:22143840-22143859 None:intergenic 45.0%
GCTTTGCATCACACACTCTT+GGG - Chr8:22144204-22144223 None:intergenic 45.0%
GTTAGTAGTTCTCTCTCCCT+CGG - Chr8:22143544-22143563 None:intergenic 45.0%
GTTTAGACTCTTGCCTGCAA+TGG + Chr8:22144496-22144515 MsG0880043294.01.T01:intron 45.0%
TCACCCTATCAGTATCAACC+TGG + Chr8:22142974-22142993 MsG0880043294.01.T01:CDS 45.0%
TCTGAATGATGTTGTGGGTC+AGG - Chr8:22144391-22144410 None:intergenic 45.0%
TGGGATGAGAGAGATGAAAC+AGG + Chr8:22145859-22145878 MsG0880043294.01.T01:intron 45.0%
TGTGCACTATTGATCCCTGT+AGG - Chr8:22142950-22142969 None:intergenic 45.0%
TTGCAGGAACTGGAGAAAAC+AGG + Chr8:22145185-22145204 MsG0880043294.01.T01:intron 45.0%
! AGTTTTCAGACCGTAGGACA+AGG - Chr8:22145431-22145450 None:intergenic 45.0%
! ATTGTTGTTCGAATGCCGGA+CGG + Chr8:22145269-22145288 MsG0880043294.01.T01:CDS 45.0%
! GCTCATGCTTTATTAGCCGA+GGG + Chr8:22143525-22143544 MsG0880043294.01.T01:CDS 45.0%
! TGCTCATGCTTTATTAGCCG+AGG + Chr8:22143524-22143543 MsG0880043294.01.T01:CDS 45.0%
!! GTATGATCAAGTTGGTCCCT+TGG + Chr8:22145525-22145544 MsG0880043294.01.T01:intron 45.0%
!! TATGCAGGTGCTGATACCAA+TGG + Chr8:22143004-22143023 MsG0880043294.01.T01:CDS 45.0%
!!! TGGTGGCATGGTTTTAAGTG+CGG + Chr8:22145545-22145564 MsG0880043294.01.T01:intron 45.0%
AACCTGGTAAGGAATGGGCA+TGG - Chr8:22143851-22143870 None:intergenic 50.0%
ATGGAATGACCATGGCTGCA+CGG - Chr8:22143686-22143705 None:intergenic 50.0%
ATGGTTACCACAACTCCTGG+AGG - Chr8:22145496-22145515 None:intergenic 50.0%
ATTCCACTCGCACATTGTGC+AGG - Chr8:22146298-22146317 None:intergenic 50.0%
GAGGCAAGGGAATTTCCTAC+AGG + Chr8:22142932-22142951 MsG0880043294.01.T01:CDS 50.0%
GATGTTGCACAACCAGTCAC+TGG + Chr8:22144175-22144194 MsG0880043294.01.T01:intron 50.0%
GCACACTCTTCCAACTTGCA+AGG + Chr8:22145465-22145484 MsG0880043294.01.T01:intron 50.0%
TCAAGTAGTACACCACCACG+TGG + Chr8:22142896-22142915 MsG0880043294.01.T01:CDS 50.0%
TCGCCTAAGGGTGGTTCATT+TGG - Chr8:22145233-22145252 None:intergenic 50.0%
TGGTTACCACAACTCCTGGA+GGG - Chr8:22145495-22145514 None:intergenic 50.0%
TGTTGTGGGTCAGGCAAAGA+TGG - Chr8:22144382-22144401 None:intergenic 50.0%
! ACTGGATGAACCCTGCCTTT+TGG - Chr8:22144428-22144447 None:intergenic 50.0%
! CTGGATGAACCCTGCCTTTT+GGG - Chr8:22144427-22144446 None:intergenic 50.0%
!! GGGGTTGGTAGGAGTACAAA+AGG - Chr8:22145396-22145415 None:intergenic 50.0%
!! TGATCAAGTTGGTCCCTTGG+TGG + Chr8:22145528-22145547 MsG0880043294.01.T01:intron 50.0%
!!! GCTTTTTCATCGCCTAAGGG+TGG - Chr8:22145242-22145261 None:intergenic 50.0%
AATTCCCTTGCCTCCCCTTG+TGG - Chr8:22142926-22142945 None:intergenic 55.0%
ACCACGTGGTTCTCCACAAG+GGG + Chr8:22142910-22142929 MsG0880043294.01.T01:CDS 55.0%
ACTCTTGGGTCTCCAGTGAC+TGG - Chr8:22144190-22144209 None:intergenic 55.0%
ACTTGCAAGGAACACCCTCC+AGG + Chr8:22145478-22145497 MsG0880043294.01.T01:intron 55.0%
ATGAAGGAACCGTGCAGCCA+TGG + Chr8:22143674-22143693 MsG0880043294.01.T01:intron 55.0%
CACCACGTGGTTCTCCACAA+GGG + Chr8:22142909-22142928 MsG0880043294.01.T01:CDS 55.0%
CCACATCGCGATTGCAGTTC+AGG + Chr8:22145619-22145638 MsG0880043294.01.T01:intron 55.0%
CCTGAACTGCAATCGCGATG+TGG - Chr8:22145622-22145641 None:intergenic 55.0%
GTCCATGCCCATTCCTTACC+AGG + Chr8:22143846-22143865 MsG0880043294.01.T01:intron 55.0%
TACGGAGCTTCGAGCGAAAG+AGG + Chr8:22143485-22143504 MsG0880043294.01.T01:CDS 55.0%
TGACCTGCACAATGTGCGAG+TGG + Chr8:22146292-22146311 MsG0880043294.01.T01:CDS 55.0%
TTCTCCACAAGGGGAGGCAA+GGG + Chr8:22142919-22142938 MsG0880043294.01.T01:CDS 55.0%
! AAGTTGGTCCCTTGGTGGCA+TGG + Chr8:22145533-22145552 MsG0880043294.01.T01:intron 55.0%
! CAACCTGGATTGGGCTATGC+AGG + Chr8:22142989-22143008 MsG0880043294.01.T01:CDS 55.0%
!! GGAGGGTGTTCCTTGCAAGT+TGG - Chr8:22145478-22145497 None:intergenic 55.0%
ACGTGGTTCTCCACAAGGGG+AGG + Chr8:22142913-22142932 MsG0880043294.01.T01:CDS 60.0%
CCACCACGTGGTTCTCCACA+AGG + Chr8:22142908-22142927 MsG0880043294.01.T01:CDS 60.0%
CCTTGTGGAGAACCACGTGG+TGG - Chr8:22142911-22142930 None:intergenic 60.0%
CGTTCACAACGACCACCGTC+CGG - Chr8:22145287-22145306 None:intergenic 60.0%
GCACCTGCATAGCCCAATCC+AGG - Chr8:22142995-22143014 None:intergenic 60.0%
GGAACACCCTCCAGGAGTTG+TGG + Chr8:22145486-22145505 MsG0880043294.01.T01:intron 60.0%
GTTCTCCACAAGGGGAGGCA+AGG + Chr8:22142918-22142937 MsG0880043294.01.T01:CDS 60.0%
GTTGTTCGAATGCCGGACGG+TGG + Chr8:22145272-22145291 MsG0880043294.01.T01:CDS 60.0%
TCCCCTTGTGGAGAACCACG+TGG - Chr8:22142914-22142933 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 22142788 22146340 22142788 ID=MsG0880043294.01;Name=MsG0880043294.01
Chr8 mRNA 22142788 22146340 22142788 ID=MsG0880043294.01.T01;Parent=MsG0880043294.01;Name=MsG0880043294.01.T01;_AED=0.40;_eAED=0.45;_QI=0|0.33|0.42|1|1|1|7|0|320
Chr8 exon 22142788 22143043 22142788 ID=MsG0880043294.01.T01:exon:4569;Parent=MsG0880043294.01.T01
Chr8 exon 22143127 22143203 22143127 ID=MsG0880043294.01.T01:exon:4570;Parent=MsG0880043294.01.T01
Chr8 exon 22143293 22143375 22143293 ID=MsG0880043294.01.T01:exon:4571;Parent=MsG0880043294.01.T01
Chr8 exon 22143470 22143584 22143470 ID=MsG0880043294.01.T01:exon:4572;Parent=MsG0880043294.01.T01
Chr8 exon 22144286 22144495 22144286 ID=MsG0880043294.01.T01:exon:4573;Parent=MsG0880043294.01.T01
Chr8 exon 22145191 22145334 22145191 ID=MsG0880043294.01.T01:exon:4574;Parent=MsG0880043294.01.T01
Chr8 exon 22146263 22146340 22146263 ID=MsG0880043294.01.T01:exon:4575;Parent=MsG0880043294.01.T01
Chr8 CDS 22142788 22143043 22142788 ID=MsG0880043294.01.T01:cds;Parent=MsG0880043294.01.T01
Chr8 CDS 22143127 22143203 22143127 ID=MsG0880043294.01.T01:cds;Parent=MsG0880043294.01.T01
Chr8 CDS 22143293 22143375 22143293 ID=MsG0880043294.01.T01:cds;Parent=MsG0880043294.01.T01
Chr8 CDS 22143470 22143584 22143470 ID=MsG0880043294.01.T01:cds;Parent=MsG0880043294.01.T01
Chr8 CDS 22144286 22144495 22144286 ID=MsG0880043294.01.T01:cds;Parent=MsG0880043294.01.T01
Chr8 CDS 22145191 22145334 22145191 ID=MsG0880043294.01.T01:cds;Parent=MsG0880043294.01.T01
Chr8 CDS 22146263 22146340 22146263 ID=MsG0880043294.01.T01:cds;Parent=MsG0880043294.01.T01
Gene Sequence

>MsG0880043294.01.T01

ATGCCTTTGTTCAATGCTGCTAGAAGATTTAGACCTTCACTACTACTTGACTCAAACTATAGGAGAGAACTTAGAGATCTATGTAACGGGTTTGGTGCTACTACGCTTTCAAGTAGTACACCACCACGTGGTTCTCCACAAGGGGAGGCAAGGGAATTTCCTACAGGGATCAATAGTGCACATGTGTCACCCTATCAGTATCAACCTGGATTGGGCTATGCAGGTGCTGATACCAATGGAAATTTATCATCTCATGAGGATGCAATGCTTCTAGCTGCAATAGAAGCCTCAAAGAAAGAGATCAGAGAAAGTTCTTCACAGAGTGCTCATAATCATTCATATTATGATGTGACTCCACAAAGTCATTTGAACGAGAAGATGATGATTTGGCTCATATCATTTCACTGTCTTTACAGAGAAAGAGGAAGCAATACGGAGCTTCGAGCGAAAGAGGAAAGTAAAGTACTTGGTGCTCATGCTTTATTAGCCGAGGGAGAGAGAACTACTAACACTAACAGAAGCAAACTAGAGTGGGGTGGTATTTCTCCTGACGAGCTCAATGAAGCATTGCTGATTGAAACTGCGCTTTATGGTGAAACTTCGAATCACAGTTCTCACAAATTTCCATCTTTGCCTGACCCACAACATCATTCAGAGAAAAATGCTGATCCCAAAAGGCAGGGTTCATCCAGTTCAGCATCTCAGTTATTATCCGAGACTCAGTTGATAAGACAACAACAGGAACTGGAGAAAACAGGTGATGCAAAAAAGATTATGCTTCCAAATGAACCACCCTTAGGCGATGAAAAAGCAATTACTATTGTTGTTCGAATGCCGGACGGTGGTCGTTGTGAACGTCGCTTCTTCAAAACTGACAAGCTTCAGGATGTCACGCTTTATAGGATACTAGATGCTGACCTGCACAATGTGCGAGTGGAATTTAGTTGCACCAGTGAAATTTAA
Protein sequence

>MsG0880043294.01.T01

MPLFNAARRFRPSLLLDSNYRRELRDLCNGFGATTLSSSTPPRGSPQGEAREFPTGINSAHVSPYQYQPGLGYAGADTNGNLSSHEDAMLLAAIEASKKEIRESSSQSAHNHSYYDVTPQSHLNEKMMIWLISFHCLYRERGSNTELRAKEESKVLGAHALLAEGERTTNTNRSKLEWGGISPDELNEALLIETALYGETSNHSSHKFPSLPDPQHHSEKNADPKRQGSSSSASQLLSETQLIRQQQELEKTGDAKKIMLPNEPPLGDEKAITIVVRMPDGGRCERRFFKTDKLQDVTLYRILDADLHNVRVEFSCTSEI*