Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044444.01.T03 | YP_009141707.1 | 90.909 | 77 | 2 | 1 | 1 | 72 | 33 | 109 | 4.69E-36 | 129 |
| MsG0880044444.01.T02 | YP_009141632.1 | 92.661 | 109 | 3 | 1 | 1 | 104 | 1 | 109 | 4.70E-59 | 189 |
| MsG0880044444.01.T01 | YP_009141632.1 | 93.333 | 105 | 2 | 1 | 1 | 100 | 1 | 105 | 3.18E-58 | 184 |
| MsG0880044444.01.T04 | YP_009141631.1 | 96.97 | 66 | 2 | 0 | 1 | 66 | 1 | 66 | 4.63E-38 | 130 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044444.01.T01 | P49169 | 77.143 | 105 | 19 | 1 | 1 | 100 | 1 | 105 | 4.64E-46 | 146 |
| MsG0880044444.01.T02 | P49169 | 77.064 | 109 | 20 | 1 | 1 | 104 | 1 | 109 | 1.96E-46 | 149 |
| MsG0880044444.01.T03 | P49169 | 75.325 | 77 | 14 | 1 | 1 | 72 | 33 | 109 | 2.27E-28 | 102 |
| MsG0880044444.01.T04 | B5LMP5 | 93.939 | 66 | 4 | 0 | 1 | 66 | 1 | 66 | 4.86E-40 | 128 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044444.01.T01 | A0A0X8GKL0 | 93.333 | 105 | 2 | 1 | 1 | 100 | 1 | 105 | 1.52e-58 | 184 |
| MsG0880044444.01.T02 | A0A0F6NLD5 | 92.661 | 109 | 3 | 1 | 1 | 104 | 1 | 109 | 2.25e-59 | 189 |
| MsG0880044444.01.T02 | A0A0X8GKL0 | 92.661 | 109 | 3 | 1 | 1 | 104 | 1 | 109 | 2.25e-59 | 189 |
| MsG0880044444.01.T03 | A0A0F6NLM7 | 90.909 | 77 | 2 | 1 | 1 | 72 | 33 | 109 | 2.24e-36 | 129 |
| MsG0880044444.01.T04 | A0A4V1E3D4 | 96.970 | 66 | 2 | 0 | 1 | 66 | 1 | 66 | 2.21e-38 | 130 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180005568.01 | MsG0880044444.01 | 0.810189 | 1.304378e-50 | 7.810937e-48 |
| MsG0780041004.01 | MsG0880044444.01 | 0.803937 | 2.745967e-49 | 1.397835e-46 |
| MsG0380013933.01 | MsG0880044444.01 | 0.851935 | 6.418000e-61 | 1.319548e-57 |
| MsG0380013955.01 | MsG0880044444.01 | 0.807226 | 5.606105e-50 | 3.105346e-47 |
| MsG0380014007.01 | MsG0880044444.01 | 0.807641 | 4.576231e-50 | 2.562324e-47 |
| MsG0380014049.01 | MsG0880044444.01 | 0.871941 | 4.655789e-67 | 1.912098e-63 |
| MsG0780039975.01 | MsG0880044444.01 | 0.801466 | 8.887231e-49 | 4.247130e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044444.01.T03 | MTR_4g051350 | 69.318 | 88 | 11 | 2 | 1 | 72 | 118 | 205 | 1.26e-24 | 93.2 |
| MsG0880044444.01.T02 | MTR_4g051350 | 75.410 | 122 | 12 | 3 | 1 | 104 | 84 | 205 | 1.87e-45 | 147 |
| MsG0880044444.01.T01 | MTR_4g051350 | 76.271 | 118 | 10 | 3 | 1 | 100 | 84 | 201 | 1.67e-45 | 145 |
| MsG0880044444.01.T04 | MTR_8g045210 | 93.548 | 62 | 4 | 0 | 4 | 65 | 36 | 97 | 2.20e-38 | 122 |
| MsG0880044444.01.T04 | MTR_4g051350 | 95.082 | 61 | 3 | 0 | 1 | 61 | 1 | 61 | 5.61e-38 | 125 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044444.01.T03 | ATCG00650 | 73.016 | 63 | 15 | 1 | 1 | 63 | 33 | 93 | 4.23e-21 | 81.3 |
| MsG0880044444.01.T02 | ATCG00650 | 74.737 | 95 | 22 | 1 | 1 | 95 | 1 | 93 | 6.32e-40 | 130 |
| MsG0880044444.01.T01 | ATCG00650 | 74.737 | 95 | 22 | 1 | 1 | 95 | 1 | 93 | 8.36e-41 | 130 |
| MsG0880044444.01.T04 | ATCG00640 | 74.242 | 66 | 17 | 0 | 1 | 66 | 1 | 66 | 8.94e-33 | 107 |
Find 28 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATACGAATAACTGTTATTT+TGG | 0.125276 | 8:-42184962 | MsG0880044444.01.T01:five_prime_UTR |
| ATATTTATATTTGTTATATA+AGG | 0.230250 | 8:+42184681 | None:intergenic |
| CCTTGGCCATGAACCTCCTT+TGG | 0.334369 | 8:+42184992 | None:intergenic |
| AGATAAAGAAATAGAGAAAA+TGG | 0.338588 | 8:-42184792 | MsG0880044444.01.T01:five_prime_UTR |
| AGAGTGTTAATAAGGAATCT+AGG | 0.346320 | 8:-42184918 | MsG0880044444.01.T01:five_prime_UTR |
| TTTATAGAATTTACCTCCTA+AGG | 0.362298 | 8:+42184753 | None:intergenic |
| AATCGTATGTTTGCAACAAA+AGG | 0.396841 | 8:+42184822 | None:intergenic |
| TACAACGCAACATCCTCAAT+AGG | 0.435840 | 8:+42184168 | None:intergenic |
| ATTGTTTGTGTGTCACCCTT+AGG | 0.451271 | 8:-42184769 | MsG0880044444.01.T01:five_prime_UTR |
| AGTTTGTCCCCGATCCAATC+GGG | 0.455483 | 8:-42184524 | MsG0880044444.01.T01:CDS |
| GAGTTTGTCCCCGATCCAAT+CGG | 0.465376 | 8:-42184525 | MsG0880044444.01.T01:CDS |
| TATTCGTATATCTTTACCCT+TGG | 0.471315 | 8:+42184975 | None:intergenic |
| GAGTGTTAATAAGGAATCTA+GGG | 0.505299 | 8:-42184917 | MsG0880044444.01.T01:five_prime_UTR |
| CCAAAGGAGGTTCATGGCCA+AGG | 0.512674 | 8:-42184992 | MsG0880044444.01.T01:five_prime_UTR |
| AAAGGTGACGAATTAACAAA+AGG | 0.521079 | 8:+42184350 | None:intergenic |
| TTATAGAATTTACCTCCTAA+GGG | 0.523266 | 8:+42184754 | None:intergenic |
| ACACAATACACCTAGTCGAT+TGG | 0.524208 | 8:-42184863 | MsG0880044444.01.T01:five_prime_UTR |
| AAAATACGACAATCCTATTG+AGG | 0.528765 | 8:-42184181 | MsG0880044444.01.T02:CDS |
| ATCGTATGTTTGCAACAAAA+GGG | 0.538893 | 8:+42184823 | None:intergenic |
| GTTTGTGTGTCACCCTTAGG+AGG | 0.563114 | 8:-42184766 | MsG0880044444.01.T01:five_prime_UTR |
| TTTCTCAATTCCAATCGACT+AGG | 0.574270 | 8:+42184853 | None:intergenic |
| GAGTCGCTTAGATTTATCCA+TGG | 0.585856 | 8:+42184577 | None:intergenic |
| CAAAGGAGGTTCATGGCCAA+GGG | 0.605158 | 8:-42184991 | MsG0880044444.01.T01:five_prime_UTR |
| GTATAGGAATAAACAAACCA+TGG | 0.629025 | 8:-42184594 | MsG0880044444.01.T01:exon |
| TATCGACTTATTAGTCAACA+AGG | 0.637963 | 8:-42184467 | MsG0880044444.01.T01:CDS |
| GTTTGTCCCCGATCCAATCG+GGG | 0.647747 | 8:-42184523 | MsG0880044444.01.T01:CDS |
| TTTGTCCCCGATCCAATCGG+GGG | 0.661952 | 8:-42184522 | MsG0880044444.01.T01:CDS |
| ATCGAAACTCAAATTCAATG+CGG | 0.701196 | 8:-42184229 | MsG0880044444.01.T02:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATCCTTTTTTTATAAGAAA+TGG | - | Chr8:42184554-42184573 | MsG0880044444.01.T01:CDS | 15.0% |
| !!! | ATCCTTTTTTTATAAGAAAT+GGG | - | Chr8:42184555-42184574 | MsG0880044444.01.T01:CDS | 15.0% |
| !!! | CTTTTTTCATTATTAACAAA+AGG | + | Chr8:42184850-42184869 | None:intergenic | 15.0% |
| !! | AGATAAAGAAATAGAGAAAA+TGG | - | Chr8:42184387-42184406 | MsG0880044444.01.T01:CDS | 20.0% |
| !! | ATCCCATTTCTTATAAAAAA+AGG | + | Chr8:42184560-42184579 | None:intergenic | 20.0% |
| !! | GTTGTAAGAAAAAAGATAAT+AGG | - | Chr8:42185028-42185047 | MsG0880044444.01.T01:five_prime_UTR | 20.0% |
| !!! | AATTAATCGTTGTTGTTTTA+AGG | + | Chr8:42184770-42184789 | None:intergenic | 20.0% |
| !!! | CTCATGTTTTTATAATCAAT+TGG | + | Chr8:42184685-42184704 | None:intergenic | 20.0% |
| !!! | TATACGAATAACTGTTATTT+TGG | - | Chr8:42184217-42184236 | MsG0880044444.01.T02:CDS | 20.0% |
| !!! | TGATAAAAAGAGTGTTAATA+AGG | - | Chr8:42184253-42184272 | MsG0880044444.01.T02:CDS | 20.0% |
| !!! | TTTATAAGAAATGGGATTTT+CGG | - | Chr8:42184563-42184582 | MsG0880044444.01.T01:CDS | 20.0% |
| ! | AGTGAATCAAAAAAAACCAA+AGG | - | Chr8:42184171-42184190 | MsG0880044444.01.T02:CDS | 25.0% |
| ! | TTATAGAATTTACCTCCTAA+GGG | + | Chr8:42184428-42184447 | None:intergenic | 25.0% |
| ! | TTTATAGAATTTACCTCCTA+AGG | + | Chr8:42184429-42184448 | None:intergenic | 25.0% |
| !!! | GTTTTTTTTAGAACAGTAGT+AGG | + | Chr8:42184892-42184911 | None:intergenic | 25.0% |
| AAAATACGACAATCCTATTG+AGG | - | Chr8:42184998-42185017 | MsG0880044444.01.T01:five_prime_UTR | 30.0% | |
| AAAGGTGACGAATTAACAAA+AGG | + | Chr8:42184832-42184851 | None:intergenic | 30.0% | |
| AATCGTATGTTTGCAACAAA+AGG | + | Chr8:42184360-42184379 | None:intergenic | 30.0% | |
| ATCGAAACTCAAATTCAATG+CGG | - | Chr8:42184950-42184969 | MsG0880044444.01.T01:five_prime_UTR | 30.0% | |
| ATCGTATGTTTGCAACAAAA+GGG | + | Chr8:42184359-42184378 | None:intergenic | 30.0% | |
| GAATCAAAAAAAACCAAAGG+AGG | - | Chr8:42184174-42184193 | MsG0880044444.01.T02:CDS | 30.0% | |
| GAGTGTTAATAAGGAATCTA+GGG | - | Chr8:42184262-42184281 | MsG0880044444.01.T02:CDS | 30.0% | |
| GTATAGGAATAAACAAACCA+TGG | - | Chr8:42184585-42184604 | MsG0880044444.01.T01:exon | 30.0% | |
| TATTCGTATATCTTTACCCT+TGG | + | Chr8:42184207-42184226 | None:intergenic | 30.0% | |
| ! | AATCTAAGCAATCTTTTCGT+AGG | - | Chr8:42184632-42184651 | MsG0880044444.01.T01:five_prime_UTR | 30.0% |
| ! | AGAGTGTTAATAAGGAATCT+AGG | - | Chr8:42184261-42184280 | MsG0880044444.01.T02:CDS | 30.0% |
| !! | TATCGACTTATTAGTCAACA+AGG | - | Chr8:42184712-42184731 | MsG0880044444.01.T01:five_prime_UTR | 30.0% |
| AAAAAACCAAAGGAGGTTCA+TGG | - | Chr8:42184181-42184200 | MsG0880044444.01.T02:CDS | 35.0% | |
| TTTCTCAATTCCAATCGACT+AGG | + | Chr8:42184329-42184348 | None:intergenic | 35.0% | |
| ! | AGAAATGGGATTTTCGGTAT+AGG | - | Chr8:42184569-42184588 | MsG0880044444.01.T01:CDS | 35.0% |
| ACACAATACACCTAGTCGAT+TGG | - | Chr8:42184316-42184335 | MsG0880044444.01.T01:CDS | 40.0% | |
| ATTGTTTGTGTGTCACCCTT+AGG | - | Chr8:42184410-42184429 | MsG0880044444.01.T01:CDS | 40.0% | |
| GAGTCGCTTAGATTTATCCA+TGG | + | Chr8:42184605-42184624 | None:intergenic | 40.0% | |
| TACAACGCAACATCCTCAAT+AGG | + | Chr8:42185014-42185033 | None:intergenic | 40.0% | |
| AATCAATTGGATCCCCCGAT+TGG | + | Chr8:42184672-42184691 | None:intergenic | 45.0% | |
| !!! | ATATTTATATTTGTTATATA+AGG | + | Chr8:42184501-42184520 | None:intergenic | 5.0% |
| AGTTTGTCCCCGATCCAATC+GGG | - | Chr8:42184655-42184674 | MsG0880044444.01.T01:five_prime_UTR | 50.0% | |
| ATTGGATCCCCCGATTGGAT+CGG | + | Chr8:42184667-42184686 | None:intergenic | 50.0% | |
| CAAAGGAGGTTCATGGCCAA+GGG | - | Chr8:42184188-42184207 | MsG0880044444.01.T02:CDS | 50.0% | |
| GAGTTTGTCCCCGATCCAAT+CGG | - | Chr8:42184654-42184673 | MsG0880044444.01.T01:five_prime_UTR | 50.0% | |
| GTTTGTGTGTCACCCTTAGG+AGG | - | Chr8:42184413-42184432 | MsG0880044444.01.T01:CDS | 50.0% | |
| CCAAAGGAGGTTCATGGCCA+AGG | - | Chr8:42184187-42184206 | MsG0880044444.01.T02:CDS | 55.0% | |
| CCTTGGCCATGAACCTCCTT+TGG | + | Chr8:42184190-42184209 | None:intergenic | 55.0% | |
| GTTTGTCCCCGATCCAATCG+GGG | - | Chr8:42184656-42184675 | MsG0880044444.01.T01:five_prime_UTR | 55.0% | |
| TTGGATCCCCCGATTGGATC+GGG | + | Chr8:42184666-42184685 | None:intergenic | 55.0% | |
| TTTGTCCCCGATCCAATCGG+GGG | - | Chr8:42184657-42184676 | MsG0880044444.01.T01:five_prime_UTR | 55.0% | |
| TGGATCCCCCGATTGGATCG+GGG | + | Chr8:42184665-42184684 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 42184148 | 42185053 | 42184148 | ID=MsG0880044444.01;Name=MsG0880044444.01 |
| Chr8 | mRNA | 42184298 | 42185053 | 42184298 | ID=MsG0880044444.01.T01;Parent=MsG0880044444.01;Name=MsG0880044444.01.T01;_AED=0.48;_eAED=0.48;_QI=456|-1|1|1|-1|0|1|0|100 |
| Chr8 | exon | 42184298 | 42185053 | 42184298 | ID=MsG0880044444.01.T01:exon:39552;Parent=MsG0880044444.01.T01 |
| Chr8 | five_prime_UTR | 42184598 | 42185053 | 42184598 | ID=MsG0880044444.01.T01:five_prime_utr;Parent=MsG0880044444.01.T01 |
| Chr8 | CDS | 42184298 | 42184597 | 42184298 | ID=MsG0880044444.01.T01:cds;Parent=MsG0880044444.01.T01 |
| Chr8 | mRNA | 42184148 | 42184597 | 42184148 | ID=MsG0880044444.01.T02;Parent=MsG0880044444.01;Name=MsG0880044444.01.T02;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|149 |
| Chr8 | exon | 42184148 | 42184597 | 42184148 | ID=MsG0880044444.01.T02:exon:39553;Parent=MsG0880044444.01.T02 |
| Chr8 | CDS | 42184148 | 42184597 | 42184148 | ID=MsG0880044444.01.T02:cds;Parent=MsG0880044444.01.T02 |
| Chr8 | mRNA | 42184148 | 42184501 | 42184148 | ID=MsG0880044444.01.T03;Parent=MsG0880044444.01;Name=MsG0880044444.01.T03;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|117 |
| Chr8 | exon | 42184148 | 42184501 | 42184148 | ID=MsG0880044444.01.T03:exon:39554;Parent=MsG0880044444.01.T03 |
| Chr8 | CDS | 42184148 | 42184501 | 42184148 | ID=MsG0880044444.01.T03:cds;Parent=MsG0880044444.01.T03 |
| Chr8 | mRNA | 42184801 | 42185001 | 42184801 | ID=MsG0880044444.01.T04;Parent=MsG0880044444.01;Name=MsG0880044444.01.T04;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|66 |
| Chr8 | exon | 42184801 | 42185001 | 42184801 | ID=MsG0880044444.01.T04:exon:39555;Parent=MsG0880044444.01.T04 |
| Chr8 | CDS | 42184801 | 42185001 | 42184801 | ID=MsG0880044444.01.T04:cds;Parent=MsG0880044444.01.T04 |
| Chr8 | mRNA | 42184763 | 42184849 | 42184763 | ID=MsG0880044444.01.T05;Parent=MsG0880044444.01;Name=MsG0880044444.01.T05;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|28 |
| Chr8 | exon | 42184763 | 42184849 | 42184763 | ID=MsG0880044444.01.T05:exon:39556;Parent=MsG0880044444.01.T05 |
| Chr8 | CDS | 42184763 | 42184849 | 42184763 | ID=MsG0880044444.01.T05:cds;Parent=MsG0880044444.01.T05 |
| Gene Sequence |
| Protein sequence |
