Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044714.01.T01 | XP_039685014.1 | 81.69 | 284 | 48 | 1 | 12 | 295 | 13 | 292 | 1.80E-148 | 428 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044714.01.T01 | A0A072TR89 | 84.249 | 273 | 39 | 1 | 12 | 284 | 13 | 281 | 3.27e-147 | 424 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0780036914.01 | MsG0880044714.01 | PPI |
| MsG0880044714.01 | MsG0780041205.01 | PPI |
| MsG0880044714.01 | MsG0780041205.01 | PPI |
| MsG0880044714.01 | MsG0380016712.01 | PPI |
| MsG0780041205.01 | MsG0880044714.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044714.01.T01 | MTR_8g467500 | 84.249 | 273 | 39 | 1 | 12 | 284 | 13 | 281 | 8.29e-151 | 424 |
| MsG0880044714.01.T01 | MTR_3g093900 | 42.773 | 339 | 144 | 4 | 1 | 289 | 1 | 339 | 4.94e-76 | 243 |
| MsG0880044714.01.T01 | MTR_4g019670 | 54.217 | 249 | 114 | 0 | 5 | 253 | 6 | 254 | 2.37e-70 | 226 |
| MsG0880044714.01.T01 | MTR_7g106510 | 50.542 | 277 | 129 | 2 | 5 | 281 | 6 | 274 | 4.56e-69 | 223 |
| MsG0880044714.01.T01 | MTR_5g075380 | 33.557 | 149 | 97 | 1 | 1 | 149 | 1 | 147 | 4.33e-15 | 72.0 |
| MsG0880044714.01.T01 | MTR_3g466980 | 29.870 | 154 | 107 | 1 | 5 | 157 | 3 | 156 | 8.03e-14 | 69.7 |
| MsG0880044714.01.T01 | MTR_4g032620 | 32.787 | 122 | 82 | 0 | 5 | 126 | 3 | 124 | 7.93e-13 | 67.0 |
| MsG0880044714.01.T01 | MTR_4g031910 | 29.936 | 157 | 109 | 1 | 5 | 160 | 3 | 159 | 4.97e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044714.01.T01 | AT1G65300 | 25.843 | 178 | 131 | 1 | 4 | 180 | 1 | 178 | 1.31e-11 | 64.3 |
Find 50 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGACCAATTGACTTATTTAA+AGG | 0.147336 | 8:-48228662 | MsG0880044714.01.T01:CDS |
| CAATTGGTCAGTCATATTTA+TGG | 0.185453 | 8:+48228675 | None:intergenic |
| CCACCAATGGCTTGTGCTTT+AGG | 0.221309 | 8:-48228403 | MsG0880044714.01.T01:CDS |
| AGTAATCTTCTCAATCAATT+TGG | 0.235467 | 8:+48228891 | None:intergenic |
| TGAACATGTGGTTCACAAAA+AGG | 0.247519 | 8:-48228840 | MsG0880044714.01.T01:CDS |
| TGTGCTTTAGGCAATGAAAA+TGG | 0.308871 | 8:-48228391 | MsG0880044714.01.T01:CDS |
| TGAAAGAAACTTTGGAGGTT+TGG | 0.312955 | 8:-48228101 | MsG0880044714.01.T01:CDS |
| AATGAAAATGGACCAGATTC+TGG | 0.316175 | 8:-48228379 | MsG0880044714.01.T01:CDS |
| ATTGACTTATTTAAAGGAAA+AGG | 0.358755 | 8:-48228656 | MsG0880044714.01.T01:CDS |
| TGTCTTTCTGAAAGAAACTT+TGG | 0.374380 | 8:-48228109 | MsG0880044714.01.T01:CDS |
| TCGTAGAAAGAGATGGTATA+AGG | 0.393265 | 8:-48228869 | MsG0880044714.01.T01:CDS |
| TTTCCTTTAAATAAGTCAAT+TGG | 0.403992 | 8:+48228659 | None:intergenic |
| TCTATGGTTACCCCAGAATC+TGG | 0.423389 | 8:+48228367 | None:intergenic |
| GAAGGAGGAGGATTATTAAG+AGG | 0.427823 | 8:+48228430 | None:intergenic |
| ATGAAAATGGACCAGATTCT+GGG | 0.428827 | 8:-48228378 | MsG0880044714.01.T01:CDS |
| TTGACTTATTTAAAGGAAAA+GGG | 0.436078 | 8:-48228655 | MsG0880044714.01.T01:CDS |
| CTTTCTGAAAGAAACTTTGG+AGG | 0.463586 | 8:-48228106 | MsG0880044714.01.T01:CDS |
| CCACCACTTGAACATCCTAA+TGG | 0.463918 | 8:-48228145 | MsG0880044714.01.T01:CDS |
| ATTGACTCAAATTTGTGTAC+TGG | 0.469082 | 8:+48228715 | None:intergenic |
| CACATATCAGCACCACCATT+AGG | 0.472105 | 8:+48228130 | None:intergenic |
| TCTAATATGTTAATTTCTGA+AGG | 0.484430 | 8:-48228214 | MsG0880044714.01.T01:CDS |
| TCCTCCTTCTCCACCACCAA+TGG | 0.489629 | 8:-48228416 | MsG0880044714.01.T01:CDS |
| CAAGCCATTGGTGGTGGAGA+AGG | 0.491726 | 8:+48228412 | None:intergenic |
| GCCACAATTAGATTTGATCA+AGG | 0.498977 | 8:-48228323 | MsG0880044714.01.T01:CDS |
| ACATGCTTGTATACCACATA+AGG | 0.506136 | 8:+48228804 | None:intergenic |
| CACCTAATCGAAGTTATGGC+TGG | 0.509756 | 8:-48228934 | None:intergenic |
| TTGACTCAAATTTGTGTACT+GGG | 0.513371 | 8:+48228716 | None:intergenic |
| GAAGTTGATTATACGAGTAG+TGG | 0.517225 | 8:-48228301 | MsG0880044714.01.T01:CDS |
| AAAGCACAAGCCATTGGTGG+TGG | 0.521769 | 8:+48228406 | None:intergenic |
| TTGCCTAAAGCACAAGCCAT+TGG | 0.525436 | 8:+48228400 | None:intergenic |
| ATTGAAGATCTTGTATCACC+TGG | 0.526030 | 8:+48228766 | None:intergenic |
| CCGTATGAGCTTTGAAAATG+TGG | 0.544653 | 8:-48228458 | MsG0880044714.01.T01:CDS |
| TCAAATCTAATTGTGGCCGT+TGG | 0.548945 | 8:+48228329 | None:intergenic |
| TCCTTGATCAAATCTAATTG+TGG | 0.555598 | 8:+48228322 | None:intergenic |
| ATGCATCAAATAGAAAATGA+AGG | 0.555741 | 8:-48228565 | MsG0880044714.01.T01:CDS |
| GCCATTGGTGGTGGAGAAGG+AGG | 0.559606 | 8:+48228415 | None:intergenic |
| CCTAAAGCACAAGCCATTGG+TGG | 0.563638 | 8:+48228403 | None:intergenic |
| AGATAATGAAGAGACACTGA+TGG | 0.564706 | 8:-48228596 | MsG0880044714.01.T01:CDS |
| GGACAGATGACAACCTTATG+TGG | 0.579986 | 8:-48228817 | MsG0880044714.01.T01:CDS |
| ATAAGGAAAGATTGAACATG+TGG | 0.590214 | 8:-48228852 | MsG0880044714.01.T01:CDS |
| GATAATGAAGAGACACTGAT+GGG | 0.611760 | 8:-48228595 | MsG0880044714.01.T01:CDS |
| ATTGGTGGTGGAGAAGGAGG+AGG | 0.614141 | 8:+48228418 | None:intergenic |
| AGATTACTCGTAGAAAGAGA+TGG | 0.618390 | 8:-48228876 | MsG0880044714.01.T01:CDS |
| TTGAAGATCTTGTATCACCT+GGG | 0.643465 | 8:+48228767 | None:intergenic |
| CCATTAGGATGTTCAAGTGG+TGG | 0.643485 | 8:+48228145 | None:intergenic |
| CCACTTGAACATCCTAATGG+TGG | 0.655952 | 8:-48228142 | MsG0880044714.01.T01:CDS |
| CCACCATTAGGATGTTCAAG+TGG | 0.660357 | 8:+48228142 | None:intergenic |
| CAGAAATTAACATATTAGAG+CGG | 0.665337 | 8:+48228218 | None:intergenic |
| TGAAAATGGACCAGATTCTG+GGG | 0.665748 | 8:-48228377 | MsG0880044714.01.T01:CDS |
| ACAATGTGGAAAGCAACACA+AGG | 0.693142 | 8:-48228270 | MsG0880044714.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAGCTTTTGATAATAATAT+GGG | - | Chr8:48228786-48228805 | MsG0880044714.01.T01:CDS | 15.0% |
| !!! | TAAAGCTTTTGATAATAATA+TGG | - | Chr8:48228785-48228804 | MsG0880044714.01.T01:CDS | 15.0% |
| !! | GTTCAAAAAAAATTCATCTA+TGG | + | Chr8:48228622-48228641 | None:intergenic | 20.0% |
| !! | TCTAATATGTTAATTTCTGA+AGG | - | Chr8:48228756-48228775 | MsG0880044714.01.T01:CDS | 20.0% |
| !! | TTGACTTATTTAAAGGAAAA+GGG | - | Chr8:48228315-48228334 | MsG0880044714.01.T01:CDS | 20.0% |
| !! | TTTCCTTTAAATAAGTCAAT+TGG | + | Chr8:48228314-48228333 | None:intergenic | 20.0% |
| !!! | ATGAATTTTTTTTGAACCAA+CGG | - | Chr8:48228625-48228644 | MsG0880044714.01.T01:CDS | 20.0% |
| !!! | ATTGACTTATTTAAAGGAAA+AGG | - | Chr8:48228314-48228333 | MsG0880044714.01.T01:CDS | 20.0% |
| ! | AGTAATCTTCTCAATCAATT+TGG | + | Chr8:48228082-48228101 | None:intergenic | 25.0% |
| ! | ATGCATCAAATAGAAAATGA+AGG | - | Chr8:48228405-48228424 | MsG0880044714.01.T01:CDS | 25.0% |
| ! | CAGAAATTAACATATTAGAG+CGG | + | Chr8:48228755-48228774 | None:intergenic | 25.0% |
| !! | CTATCAATAACATTTTCATG+TGG | + | Chr8:48228732-48228751 | None:intergenic | 25.0% |
| !! | TGACCAATTGACTTATTTAA+AGG | - | Chr8:48228308-48228327 | MsG0880044714.01.T01:CDS | 25.0% |
| !!! | TCTTTAGATGATTTTGATGA+TGG | - | Chr8:48228432-48228451 | MsG0880044714.01.T01:CDS | 25.0% |
| !!! | TGTTTTGGAAAAACTTAAGA+TGG | - | Chr8:48228479-48228498 | MsG0880044714.01.T01:CDS | 25.0% |
| AAAGGGTAAAAAACTTGAAG+AGG | - | Chr8:48228332-48228351 | MsG0880044714.01.T01:CDS | 30.0% | |
| ATAAGGAAAGATTGAACATG+TGG | - | Chr8:48228118-48228137 | MsG0880044714.01.T01:CDS | 30.0% | |
| CAATTGGTCAGTCATATTTA+TGG | + | Chr8:48228298-48228317 | None:intergenic | 30.0% | |
| TCCTTGATCAAATCTAATTG+TGG | + | Chr8:48228651-48228670 | None:intergenic | 30.0% | |
| TTGACTCAAATTTGTGTACT+GGG | + | Chr8:48228257-48228276 | None:intergenic | 30.0% | |
| ! | ATTGACTCAAATTTGTGTAC+TGG | + | Chr8:48228258-48228277 | None:intergenic | 30.0% |
| ! | TGTCTTTCTGAAAGAAACTT+TGG | - | Chr8:48228861-48228880 | MsG0880044714.01.T01:CDS | 30.0% |
| !! | TCGCTTGCTTAATTTTGTTT+TGG | - | Chr8:48228464-48228483 | MsG0880044714.01.T01:CDS | 30.0% |
| AACATGTGGTTCACAAAAAG+GGG | - | Chr8:48228132-48228151 | MsG0880044714.01.T01:CDS | 35.0% | |
| AATGAAAATGGACCAGATTC+TGG | - | Chr8:48228591-48228610 | MsG0880044714.01.T01:CDS | 35.0% | |
| ACATGCTTGTATACCACATA+AGG | + | Chr8:48228169-48228188 | None:intergenic | 35.0% | |
| AGATAATGAAGAGACACTGA+TGG | - | Chr8:48228374-48228393 | MsG0880044714.01.T01:CDS | 35.0% | |
| AGATTACTCGTAGAAAGAGA+TGG | - | Chr8:48228094-48228113 | MsG0880044714.01.T01:CDS | 35.0% | |
| ATGAAAATGGACCAGATTCT+GGG | - | Chr8:48228592-48228611 | MsG0880044714.01.T01:CDS | 35.0% | |
| ATTGAAGATCTTGTATCACC+TGG | + | Chr8:48228207-48228226 | None:intergenic | 35.0% | |
| GAACATGTGGTTCACAAAAA+GGG | - | Chr8:48228131-48228150 | MsG0880044714.01.T01:CDS | 35.0% | |
| GATAATGAAGAGACACTGAT+GGG | - | Chr8:48228375-48228394 | MsG0880044714.01.T01:CDS | 35.0% | |
| GCCACAATTAGATTTGATCA+AGG | - | Chr8:48228647-48228666 | MsG0880044714.01.T01:CDS | 35.0% | |
| GGAGGTTTGGATAAAAATAC+AGG | - | Chr8:48228882-48228901 | MsG0880044714.01.T01:CDS | 35.0% | |
| TCGTAGAAAGAGATGGTATA+AGG | - | Chr8:48228101-48228120 | MsG0880044714.01.T01:CDS | 35.0% | |
| TGAACATGTGGTTCACAAAA+AGG | - | Chr8:48228130-48228149 | MsG0880044714.01.T01:CDS | 35.0% | |
| TTGAAGATCTTGTATCACCT+GGG | + | Chr8:48228206-48228225 | None:intergenic | 35.0% | |
| ! | CCACATTTTCAAAGCTCATA+CGG | + | Chr8:48228515-48228534 | None:intergenic | 35.0% |
| ! | CTTTCTGAAAGAAACTTTGG+AGG | - | Chr8:48228864-48228883 | MsG0880044714.01.T01:CDS | 35.0% |
| ! | GAAGTTGATTATACGAGTAG+TGG | - | Chr8:48228669-48228688 | MsG0880044714.01.T01:CDS | 35.0% |
| ! | TGAAAGAAACTTTGGAGGTT+TGG | - | Chr8:48228869-48228888 | MsG0880044714.01.T01:CDS | 35.0% |
| ! | TGTGCTTTAGGCAATGAAAA+TGG | - | Chr8:48228579-48228598 | MsG0880044714.01.T01:CDS | 35.0% |
| !!! | TAGTGGTTTTGATGACAATG+TGG | - | Chr8:48228686-48228705 | MsG0880044714.01.T01:CDS | 35.0% |
| ACAATGTGGAAAGCAACACA+AGG | - | Chr8:48228700-48228719 | MsG0880044714.01.T01:CDS | 40.0% | |
| GAAGGAGGAGGATTATTAAG+AGG | + | Chr8:48228543-48228562 | None:intergenic | 40.0% | |
| TCAAATCTAATTGTGGCCGT+TGG | + | Chr8:48228644-48228663 | None:intergenic | 40.0% | |
| TCACCTGGGAAAAATACAAC+AGG | + | Chr8:48228192-48228211 | None:intergenic | 40.0% | |
| TGAAAATGGACCAGATTCTG+GGG | - | Chr8:48228593-48228612 | MsG0880044714.01.T01:CDS | 40.0% | |
| ! | TGTCCTGTTGTATTTTTCCC+AGG | - | Chr8:48228186-48228205 | MsG0880044714.01.T01:CDS | 40.0% |
| !! | CCGTATGAGCTTTGAAAATG+TGG | - | Chr8:48228512-48228531 | MsG0880044714.01.T01:CDS | 40.0% |
| CACATATCAGCACCACCATT+AGG | + | Chr8:48228843-48228862 | None:intergenic | 45.0% | |
| CCACCACTTGAACATCCTAA+TGG | - | Chr8:48228825-48228844 | MsG0880044714.01.T01:CDS | 45.0% | |
| CCACCATTAGGATGTTCAAG+TGG | + | Chr8:48228831-48228850 | None:intergenic | 45.0% | |
| CCACTTGAACATCCTAATGG+TGG | - | Chr8:48228828-48228847 | MsG0880044714.01.T01:CDS | 45.0% | |
| CCATTAGGATGTTCAAGTGG+TGG | + | Chr8:48228828-48228847 | None:intergenic | 45.0% | |
| GGACAGATGACAACCTTATG+TGG | - | Chr8:48228153-48228172 | MsG0880044714.01.T01:CDS | 45.0% | |
| TCTATGGTTACCCCAGAATC+TGG | + | Chr8:48228606-48228625 | None:intergenic | 45.0% | |
| TTGCCTAAAGCACAAGCCAT+TGG | + | Chr8:48228573-48228592 | None:intergenic | 45.0% | |
| AAAGCACAAGCCATTGGTGG+TGG | + | Chr8:48228567-48228586 | None:intergenic | 50.0% | |
| CCTAAAGCACAAGCCATTGG+TGG | + | Chr8:48228570-48228589 | None:intergenic | 50.0% | |
| ! | CCACCAATGGCTTGTGCTTT+AGG | - | Chr8:48228567-48228586 | MsG0880044714.01.T01:CDS | 50.0% |
| TCCTCCTTCTCCACCACCAA+TGG | - | Chr8:48228554-48228573 | MsG0880044714.01.T01:CDS | 55.0% | |
| !! | ATTGGTGGTGGAGAAGGAGG+AGG | + | Chr8:48228555-48228574 | None:intergenic | 55.0% |
| !! | CAAGCCATTGGTGGTGGAGA+AGG | + | Chr8:48228561-48228580 | None:intergenic | 55.0% |
| !! | GCCATTGGTGGTGGAGAAGG+AGG | + | Chr8:48228558-48228577 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 48228051 | 48228941 | 48228051 | ID=MsG0880044714.01;Name=MsG0880044714.01 |
| Chr8 | mRNA | 48228051 | 48228941 | 48228051 | ID=MsG0880044714.01.T01;Parent=MsG0880044714.01;Name=MsG0880044714.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|296 |
| Chr8 | exon | 48228051 | 48228941 | 48228051 | ID=MsG0880044714.01.T01:exon:19922;Parent=MsG0880044714.01.T01 |
| Chr8 | CDS | 48228051 | 48228941 | 48228051 | ID=MsG0880044714.01.T01:cds;Parent=MsG0880044714.01.T01 |
| Gene Sequence |
| Protein sequence |
