AlfalfaGEDB | MsG0880044963.01

M. sativa cultivar ZhongmuNo.1 / MsG0880044963.01

KEGG: -
GO: GO:0016021
PFAM: PF05758

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044963.01.T01	YP_009657472.1	87.368	190	18	1	1	190	218	401	1.81E-101	332

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044963.01.T01	Q9BBN6	50.526	190	85	2	1	190	205	385	1.37E-45	163

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044963.01.T01	A0A4P8F4W3	87.368	190	18	1	1	190	218	401	8.66e-102	332

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0380013922.01	MsG0880044963.01	0.835886	1.292044e-56	1.601328e-53
MsG0780037769.01	MsG0880044963.01	0.850460	1.674280e-60	3.281965e-57
MsG0780038139.01	MsG0880044963.01	0.807650	4.557532e-50	2.552413e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044963.01.T01	MTR_4g006080	80.208	192	25	3	1	190	215	395	1.55e-87	275
MsG0880044963.01.T01	MTR_5g039110	77.419	93	20	1	76	167	83	175	1.73e-43	143
MsG0880044963.01.T01	MTR_1g110440	85.106	47	5	1	1	45	117	163	4.27e-20	82.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880044963.01.T01	ATCG01130	36.946	203	102	6	5	183	214	414	1.21e-32	124

Find 17 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCTCTTTCAACATTGATTTC+AGG	0.186194	8:-52146091	None:intergenic
GTTATCCTTGGCCACTATTT+AGG	0.265875	8:+52145989	MsG0880044963.01.T01:CDS
TTCAGAAAACTAGATAAATC+AGG	0.403925	8:-52146430	None:intergenic
GTTACAATTGCGAAATTATG+TGG	0.414197	8:+52145940	MsG0880044963.01.T01:CDS
TCGATTATCGAAAATGGAAA+AGG	0.440245	8:+52146290	MsG0880044963.01.T01:CDS
AATCGATGTTGAAATAGATT+CGG	0.466965	8:+52146114	MsG0880044963.01.T01:CDS
TGTTGAAATAGATTCGGAAA+TGG	0.470477	8:+52146120	MsG0880044963.01.T01:CDS
AAGATCTTCTTCGTCTTCGA+TGG	0.497764	8:-52146170	None:intergenic
TTTCATATATGTCAAAGTGA+TGG	0.515403	8:+52146379	MsG0880044963.01.T01:CDS
AGATAATAATCTCTTCGCAT+TGG	0.518704	8:+52146243	MsG0880044963.01.T01:CDS
TCGTACGAGATGAAAATTCA+CGG	0.551569	8:+52146350	MsG0880044963.01.T01:CDS
GAAACTAAACAAGAACAAAA+CGG	0.558977	8:+52146145	MsG0880044963.01.T01:CDS
TTCTGCCTAAATAGTGGCCA+AGG	0.562675	8:-52145994	None:intergenic
TCGAAAAGAGTAGTTGCAAG+AGG	0.575559	8:-52146271	None:intergenic
TTACAATTGCGAAATTATGT+GGG	0.597433	8:+52145941	MsG0880044963.01.T01:CDS
GCGGTGTTCTGCCTAAATAG+TGG	0.611852	8:-52146000	None:intergenic
AGAAAACTAGATAAATCAGG+TGG	0.627307	8:-52146427	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTTCATCAGTATAAAAATA+TGG	-	Chr8:52146028-52146047	None:intergenic	15.0%
!	AATCGATGTTGAAATAGATT+CGG	+	Chr8:52146114-52146133	MsG0880044963.01.T01:CDS	25.0%
!	CTGAAAATCATGGAAAAAAA+TGG	+	Chr8:52146448-52146467	MsG0880044963.01.T01:CDS	25.0%
!	GAAACTAAACAAGAACAAAA+CGG	+	Chr8:52146145-52146164	MsG0880044963.01.T01:CDS	25.0%
!	TCAGTATAAAAATATGGAAG+CGG	-	Chr8:52146022-52146041	None:intergenic	25.0%
!	TTACAATTGCGAAATTATGT+GGG	+	Chr8:52145941-52145960	MsG0880044963.01.T01:CDS	25.0%
!	TTCAGAAAACTAGATAAATC+AGG	-	Chr8:52146433-52146452	None:intergenic	25.0%
!	TTTCATATATGTCAAAGTGA+TGG	+	Chr8:52146379-52146398	MsG0880044963.01.T01:CDS	25.0%
!!!	ATCTAGTTTTCTGAAAATCA+TGG	+	Chr8:52146438-52146457	MsG0880044963.01.T01:CDS	25.0%
!!!	GTTTTTTCATTTGTTATCCT+TGG	+	Chr8:52145977-52145996	MsG0880044963.01.T01:CDS	25.0%
!!!	TCCAAAGTCTTATCTTTTTT+CGG	-	Chr8:52146211-52146230	None:intergenic	25.0%
!!!	TCGTTTTTTATATATCGCAA+TGG	-	Chr8:52146316-52146335	None:intergenic	25.0%
	AGAAAACTAGATAAATCAGG+TGG	-	Chr8:52146430-52146449	None:intergenic	30.0%
	AGATAATAATCTCTTCGCAT+TGG	+	Chr8:52146243-52146262	MsG0880044963.01.T01:CDS	30.0%
	GTTACAATTGCGAAATTATG+TGG	+	Chr8:52145940-52145959	MsG0880044963.01.T01:CDS	30.0%
	TCCGAAAAAAGATAAGACTT+TGG	+	Chr8:52146207-52146226	MsG0880044963.01.T01:CDS	30.0%
	TCGATTATCGAAAATGGAAA+AGG	+	Chr8:52146290-52146309	MsG0880044963.01.T01:CDS	30.0%
	TGTTGAAATAGATTCGGAAA+TGG	+	Chr8:52146120-52146139	MsG0880044963.01.T01:CDS	30.0%
!	CTCTTTTCGATTATCGAAAA+TGG	+	Chr8:52146284-52146303	MsG0880044963.01.T01:CDS	30.0%
!!	TCTCTTTCAACATTGATTTC+AGG	-	Chr8:52146094-52146113	None:intergenic	30.0%
	TCGTACGAGATGAAAATTCA+CGG	+	Chr8:52146350-52146369	MsG0880044963.01.T01:CDS	35.0%
	AAGATCTTCTTCGTCTTCGA+TGG	-	Chr8:52146173-52146192	None:intergenic	40.0%
	GTTATCCTTGGCCACTATTT+AGG	+	Chr8:52145989-52146008	MsG0880044963.01.T01:CDS	40.0%
	TCGAAAAGAGTAGTTGCAAG+AGG	-	Chr8:52146274-52146293	None:intergenic	40.0%
	TTCTGCCTAAATAGTGGCCA+AGG	-	Chr8:52145997-52146016	None:intergenic	45.0%
	GCGGTGTTCTGCCTAAATAG+TGG	-	Chr8:52146003-52146022	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	52145917	52146489	52145917	ID=MsG0880044963.01;Name=MsG0880044963.01
Chr8	mRNA	52145917	52146489	52145917	ID=MsG0880044963.01.T01;Parent=MsG0880044963.01;Name=MsG0880044963.01.T01;_AED=0.41;_eAED=0.41;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|190
Chr8	exon	52145917	52146489	52145917	ID=MsG0880044963.01.T01:exon:22246;Parent=MsG0880044963.01.T01
Chr8	CDS	52145917	52146489	52145917	ID=MsG0880044963.01.T01:cds;Parent=MsG0880044963.01.T01

Gene Sequence

>MsG0880044963.01.T01

ATGCGATTTGATAAGTACATTCTGTTACAATTGCGAAATTATGTGGGTCAAATATTTGTTGTTTTTTCATTTGTTATCCTTGGCCACTATTTAGGCAGAACACCGCTTCCATATTTTTATACTGATGAAATCTTAGAAGAAGAATTAGAATACGAATACGAAGAGAATGAAAAGCCTGAAATCAATGTTGAAAGAGAAATCGATGTTGAAATAGATTCGGAAATGGATGAAACTAAACAAGAACAAAACGGCTCCATCGAAGACGAAGAAGATCTTATTTCTTATCTTTTTCCGAAAAAAGATAAGACTTTGGACAACATTGAGCAAGATAATAATCTCTTCGCATTGGAAAAACCTCTTGCAACTACTCTTTTCGATTATCGAAAATGGAAAAGGCCATTGCGATATATAAAAAACGATCACGTTGAAAGAGTCGTACGAGATGAAAATTCACGGTTTTTTTTTCATATATGTCAAAGTGATGGAAAAGAACGAATATCTTTCACGTATCCACCTGATTTATCTAGTTTTCTGAAAATCATGGAAAAAAATGGATCTCTTCACAAGAGATAA

Protein sequence

>MsG0880044963.01.T01

MRFDKYILLQLRNYVGQIFVVFSFVILGHYLGRTPLPYFYTDEILEEELEYEYEENEKPEINVEREIDVEIDSEMDETKQEQNGSIEDEEDLISYLFPKKDKTLDNIEQDNNLFALEKPLATTLFDYRKWKRPLRYIKNDHVERVVRDENSRFFFHICQSDGKERISFTYPPDLSSFLKIMEKNGSLHKR*