AlfalfaGEDB | MsG0880045103.01

M. sativa cultivar ZhongmuNo.1 / MsG0880045103.01

KEGG: -
GO: GO:0005515
PFAM: PF12796 PF13962

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045103.01.T01	AES77856.1	71.893	338	80	2	4	332	3	334	6.63E-169	488

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045103.01.T01	Q8GYH5	36.422	313	165	6	7	295	2	304	9.80E-60	202

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045103.01.T01	G7KSX2	71.893	338	80	2	4	332	3	334	3.17e-169	488

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0080048977.01	MsG0880045103.01	PPI
MsG0180001046.01	MsG0880045103.01	PPI
MsG0180001047.01	MsG0880045103.01	PPI
MsG0180001883.01	MsG0880045103.01	PPI
MsG0180001883.01	MsG0880045103.01	PPI
MsG0180001883.01	MsG0880045103.01	PPI
MsG0180001883.01	MsG0880045103.01	PPI
MsG0180001883.01	MsG0880045103.01	PPI
MsG0180001883.01	MsG0880045103.01	PPI
MsG0180002432.01	MsG0880045103.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045103.01.T01	MTR_7g021090	71.893	338	80	2	4	332	3	334	8.03e-173	488
MsG0880045103.01.T01	MTR_8g039620	71.137	343	86	3	2	332	6	347	3.17e-171	486
MsG0880045103.01.T01	MTR_8g072510	72.222	342	83	4	1	332	1	340	1.92e-167	476
MsG0880045103.01.T01	MTR_1g059930	69.706	340	94	1	8	338	10	349	4.20e-160	457
MsG0880045103.01.T01	MTR_8g062410	69.388	343	86	4	1	332	1	335	1.32e-157	451
MsG0880045103.01.T01	MTR_3g016210	65.730	356	97	5	1	332	1	355	1.61e-154	443
MsG0880045103.01.T01	MTR_8g068990	63.501	337	96	5	8	332	10	331	3.03e-143	415
MsG0880045103.01.T01	MTR_8g073260	60.694	346	123	4	1	336	1	343	1.97e-142	413
MsG0880045103.01.T01	MTR_7g451420	65.916	311	95	3	4	304	3	312	4.92e-138	397
MsG0880045103.01.T01	MTR_8g069880	62.463	341	114	6	6	335	3	340	3.14e-130	382

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045103.01.T01	AT4G10720	41.867	332	156	4	7	311	2	323	4.38e-82	257
MsG0880045103.01.T01	AT4G10720	41.867	332	156	4	7	311	2	323	7.87e-82	258
MsG0880045103.01.T01	AT1G14500	37.500	320	182	4	7	308	2	321	8.17e-70	226
MsG0880045103.01.T01	AT5G15500	41.083	314	167	6	7	302	2	315	1.00e-69	226
MsG0880045103.01.T01	AT1G14480	37.798	336	186	5	7	321	2	335	4.12e-67	219
MsG0880045103.01.T01	AT5G54620	40.260	308	168	3	7	298	2	309	3.59e-66	217
MsG0880045103.01.T01	AT5G54610	36.422	313	165	6	7	295	2	304	9.99e-61	202
MsG0880045103.01.T01	AT1G14480	33.229	319	181	5	7	321	2	292	1.17e-48	170
MsG0880045103.01.T01	AT5G15500	36.058	208	116	5	112	302	2	209	1.59e-29	117
MsG0880045103.01.T01	AT5G51160	29.562	274	156	5	64	307	35	301	3.45e-29	118

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Find 85 sgRNAs with CRISPR-Local

Find 315 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGGAATATTAAACAAG+AGG	0.615959	8:-54281857	MsG0880045103.01.T01:CDS
AAAGATCTTGTTAGAGTTAA+AGG	0.117136	8:-54282095	MsG0880045103.01.T01:CDS
AAAGTATGAGAGAGCTTTGA+AGG	0.458415	8:-54277306	MsG0880045103.01.T01:CDS
AAATGGAATATTAAACAAGA+GGG	0.596917	8:-54281856	MsG0880045103.01.T01:CDS
AAATTTGAACTCAATGAACA+TGG	0.614702	8:-54280121	MsG0880045103.01.T01:CDS
AACCTGATAAATTCCACCAG+GGG	0.693447	8:+54279870	None:intergenic
AAGTATGAGAGAGCTTTGAA+GGG	0.647957	8:-54277305	MsG0880045103.01.T01:CDS
AATCGGTTATCATTATTTGT+GGG	0.501672	8:+54282386	None:intergenic
ACCGATTTCCCAACATTTCT+TGG	0.210830	8:+54279790	None:intergenic
ACCGGTCACCATGTCACGAG+TGG	0.640889	8:-54277270	MsG0880045103.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAGATGTCATTAGGATGA+TGG	+	Chr8:54278706-54278725	None:intergenic	30.0%
AAATAGAAATTGTCCATCCT+TGG	-	Chr8:54281256-54281275	MsG0880045103.01.T01:intron	30.0%
AATACTCATAGATTGTACCA+CGG	-	Chr8:54280495-54280514	MsG0880045103.01.T01:intron	30.0%
AATGAGTAACTTTCATGGTA+TGG	-	Chr8:54279323-54279342	MsG0880045103.01.T01:intron	30.0%
ACAAAGAAGACATGGTATAT+TGG	-	Chr8:54280625-54280644	MsG0880045103.01.T01:intron	30.0%
ACATTTGTTCTTCATGTAAG+AGG	-	Chr8:54281350-54281369	MsG0880045103.01.T01:intron	30.0%
ACTTCCCATTCGTAAAAAAT+CGG	-	Chr8:54282070-54282089	MsG0880045103.01.T01:CDS	30.0%
AGACATTGTATATGAGACAA+GGG	-	Chr8:54282139-54282158	MsG0880045103.01.T01:CDS	30.0%
AGTATTTGATTACGATTAGG+GGG	+	Chr8:54278761-54278780	None:intergenic	30.0%
ATACCATGAAAGTTACTCAT+TGG	+	Chr8:54279324-54279343	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	54277166	54282414	54277166	ID=MsG0880045103.01;Name=MsG0880045103.01
Chr8	mRNA	54277166	54282414	54277166	ID=MsG0880045103.01.T01;Parent=MsG0880045103.01;Name=MsG0880045103.01.T01;_AED=0.42;_eAED=0.42;_QI=0\|0\|0\|1\|1\|1\|3\|0\|394
Chr8	exon	54281803	54282414	54281803	ID=MsG0880045103.01.T01:exon:23420;Parent=MsG0880045103.01.T01
Chr8	exon	54279792	54280172	54279792	ID=MsG0880045103.01.T01:exon:23419;Parent=MsG0880045103.01.T01
Chr8	exon	54277166	54277357	54277166	ID=MsG0880045103.01.T01:exon:23418;Parent=MsG0880045103.01.T01
Chr8	CDS	54281803	54282414	54281803	ID=MsG0880045103.01.T01:cds;Parent=MsG0880045103.01.T01
Chr8	CDS	54279792	54280172	54279792	ID=MsG0880045103.01.T01:cds;Parent=MsG0880045103.01.T01
Chr8	CDS	54277166	54277357	54277166	ID=MsG0880045103.01.T01:cds;Parent=MsG0880045103.01.T01

Gene Sequence

>MsG0880045103.01.T01

ATGAATCCCACAAATAATGATAACCGATTGAAAGCTGCAGCTCAAGCAGGTGATATAGATCTCCTCTACACAGTGATTCAAGATGATCCATCCATTTTGGAGCGTATAGATTTAATACCATTTGTTGAAACTCCTTTACATATTGCTGCATCTATGGGTCATCTCCCATTTGCCACCGAAATTATGAATTTAAAACCTTCATTTGCATGGAAGCTAAACCCGCAAGGATCCAGCCCCATCCATCTCGCTATGCAAAAAAACCAAAAACGTATGGTGTATCGCTTTGTGAACATCAATAAAGATCTTGTTAGAGTTAAAGGAAGAGAATGCTTTACTCCTCTTCATTATGCAAGTCAAATCGGTGAGGTTGATCTTTTAACTAAATTTCTCTTTTCGTGTCCGGGATCTATTGAATACTTGACCATAAGAGGTGAGACTGCGCTGCATATTGCTGTTAAGTACGAACAATATGAGGCCCTTAAAGTACTTGTTAGCTGGCTCAAAAAAAATAAACGGAGAGGCGCTCAAAAGCTGGAAAATGGAATATTAAACAAGAGGGACGATGCAGGCAACACCATTTTGCATATTTCAGCCCTAAGGAGTGAGCTGCAGGCACTTCAATTGTTGGTAGAGACTAATATAAATTTGAACTCAATGAACATGGAGAGCAAAACAGCATTGGACATAGCAAGCACTCCAGAGATCAAGAGTATATTAGAAGTCGCTGATGCTCCCACACTTGCACATAAGCTCAAGTCATCAAAGACCAGAATTAGAGGTAATATGTCAACTTATTTAAGGATTAGAGCAAATATGACAGAGGAGAAGCGTAACACTTGGTTGATAGTTGCGACTCTTGTTGCAACTGCGATCTATCAATCAGGATTGAGTCCCCCTGGTGGAATTTATCAGGTTAGTGCTGGTGATGACAATGGTGTAAACATTTTCTCAAATTCTACTATTTCTTCTACTCCAAGAAATGTTGGGAAATCGGTAAAGATGGAAGCGGTGTTAATACAACAAAAGTATGAGAGAGCTTTGAAGGGTGAAGGTTCGTTACCGGTCACCATGTCACGAGTGGAGAAGACCGAGATGGTGGACAAGGCCAGAAGTGCCATTGTCTTGTGCCTCGGGGATAAAGTCTTGAGAGAAGTAGCGAAGGAACCAACTGCGGCATTGATGTGA

Protein sequence

>MsG0880045103.01.T01

MNPTNNDNRLKAAAQAGDIDLLYTVIQDDPSILERIDLIPFVETPLHIAASMGHLPFATEIMNLKPSFAWKLNPQGSSPIHLAMQKNQKRMVYRFVNINKDLVRVKGRECFTPLHYASQIGEVDLLTKFLFSCPGSIEYLTIRGETALHIAVKYEQYEALKVLVSWLKKNKRRGAQKLENGILNKRDDAGNTILHISALRSELQALQLLVETNINLNSMNMESKTALDIASTPEIKSILEVADAPTLAHKLKSSKTRIRGNMSTYLRIRANMTEEKRNTWLIVATLVATAIYQSGLSPPGGIYQVSAGDDNGVNIFSNSTISSTPRNVGKSVKMEAVLIQQKYERALKGEGSLPVTMSRVEKTEMVDKARSAIVLCLGDKVLREVAKEPTAALM*