AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045173.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045173.01.T01 MTR_8g066820 97.107 242 0 1 1 242 1 235 1.45e-177 487
MsG0880045173.01.T01 MTR_5g030130 82.449 245 32 4 1 242 1 237 9.33e-146 407
MsG0880045173.01.T01 MTR_5g007230 70.423 213 54 3 28 240 9 212 7.31e-107 307
MsG0880045173.01.T01 MTR_5g030130 84.393 173 18 2 1 171 1 166 3.58e-106 305
MsG0880045173.01.T01 MTR_4g078170 72.059 204 53 4 38 240 13 213 4.29e-104 300
MsG0880045173.01.T01 MTR_1g062160 55.340 206 73 5 40 236 7 202 5.50e-73 221
MsG0880045173.01.T01 MTR_7g102790 58.333 204 71 5 40 236 7 203 3.58e-71 216
MsG0880045173.01.T01 MTR_2g060540 49.630 135 58 4 41 165 11 145 9.38e-39 132
MsG0880045173.01.T01 MTR_2g060500 48.148 135 60 3 41 165 11 145 1.86e-38 131
MsG0880045173.01.T01 MTR_4g116300 48.031 127 65 1 40 166 6 131 7.77e-37 130
MsG0880045173.01.T01 MTR_2g084495 34.052 232 109 6 41 235 5 229 3.44e-36 128
MsG0880045173.01.T01 MTR_2g055210 32.922 243 117 5 37 235 99 339 1.83e-35 129
MsG0880045173.01.T01 MTR_4g094680 33.478 230 118 7 33 235 15 236 7.22e-35 125
MsG0880045173.01.T01 MTR_2g084525 46.715 137 63 4 41 167 5 141 9.01e-32 117
MsG0880045173.01.T01 MTR_2g084510 43.066 137 68 3 41 167 4 140 1.40e-30 114
MsG0880045173.01.T01 MTR_2g036170 31.532 222 119 6 40 235 37 251 8.82e-28 107
MsG0880045173.01.T01 MTR_8g105930 34.932 146 72 5 42 166 15 158 3.63e-16 75.5
MsG0880045173.01.T01 MTR_8g097310 29.193 161 99 4 79 235 4 153 2.03e-15 71.6
MsG0880045173.01.T01 MTR_5g077740 26.866 201 105 8 40 235 23 186 7.77e-15 70.9
MsG0880045173.01.T01 MTR_1g032880 33.929 112 60 4 86 187 27 134 5.17e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045173.01.T01 AT4G17900 71.250 240 52 6 1 236 1 227 1.48e-119 340
MsG0880045173.01.T01 AT4G17900 72.906 203 46 4 37 236 17 213 8.26e-104 300
MsG0880045173.01.T01 AT1G32700 70.968 217 53 5 31 242 2 213 2.26e-103 298
MsG0880045173.01.T01 AT1G32700 70.391 179 44 4 68 242 1 174 6.81e-80 238
MsG0880045173.01.T01 AT1G21000 50.209 239 82 4 33 235 9 246 7.68e-77 233
MsG0880045173.01.T01 AT1G21000 50.209 239 82 4 33 235 6 243 7.95e-77 232
MsG0880045173.01.T01 AT5G46710 51.020 245 90 8 1 235 1 225 9.42e-77 231
MsG0880045173.01.T01 AT1G76590 50.877 228 80 4 40 235 18 245 2.88e-74 226
MsG0880045173.01.T01 AT5G46710 52.830 212 84 6 33 235 5 209 3.52e-72 219
MsG0880045173.01.T01 AT5G46710 57.143 168 57 3 1 166 1 155 2.37e-65 201
MsG0880045173.01.T01 AT1G43000 46.083 217 101 4 33 235 9 223 4.48e-62 194
MsG0880045173.01.T01 AT1G43000 46.083 217 101 4 33 235 2 216 5.05e-62 194
MsG0880045173.01.T01 AT2G27930 44.712 208 83 5 40 237 4 189 6.25e-53 169
MsG0880045173.01.T01 AT2G27930 44.712 208 83 5 40 237 4 189 6.25e-53 169
MsG0880045173.01.T01 AT2G27930 55.474 137 51 2 40 166 4 140 1.07e-48 158
MsG0880045173.01.T01 AT2G27930 55.474 137 51 2 40 166 4 140 1.07e-48 158
MsG0880045173.01.T01 AT2G12646 48.819 127 64 1 40 166 3 128 3.11e-37 131
MsG0880045173.01.T01 AT2G12646 48.819 127 64 1 40 166 6 131 3.36e-37 131
MsG0880045173.01.T01 AT2G12646 48.819 127 64 1 40 166 6 131 3.36e-37 131
MsG0880045173.01.T01 AT3G60670 37.949 195 105 3 35 228 2 181 3.34e-36 128
MsG0880045173.01.T01 AT1G31040 34.513 226 116 5 40 235 19 242 2.47e-34 124
MsG0880045173.01.T01 AT2G27930 45.455 154 53 4 93 237 61 192 6.70e-34 121
MsG0880045173.01.T01 AT2G27930 45.455 154 53 4 93 237 61 192 6.70e-34 121
MsG0880045173.01.T01 AT2G01818 34.579 214 115 9 42 235 13 221 4.63e-22 91.3
MsG0880045173.01.T01 AT2G01818 33.987 153 81 7 99 235 9 157 9.35e-11 58.9

Find 94 sgRNAs with CRISPR-Local

Find 205 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGAATTCCCTTTCTTCTTT+TGG 0.193854 8:+55147319 None:intergenic
CAAAGATCATCGTGCTATTC+AGG 0.214126 8:-55148900 MsG0880045173.01.T01:intron
TGTATGTGTGTGTTGTTTAT+TGG 0.237468 8:+55149897 None:intergenic
AGTAAGTTATATGTAAGTTT+TGG 0.253096 8:-55146766 MsG0880045173.01.T01:three_prime_UTR
GAGCTGACTTGTTCTTCTTC+TGG 0.262316 8:+55147468 None:intergenic
TAGGGGAGTTCGACTCATTA+AGG 0.273487 8:+55147221 None:intergenic
CCTTGCACTGTTGATAATAT+AGG 0.282719 8:+55148308 None:intergenic
AGTGGTTGTTCAATCAAAAT+TGG 0.296288 8:+55147132 None:intergenic
CTTCATACCATGATGTGATT+AGG 0.304121 8:-55148374 MsG0880045173.01.T01:CDS
ATCTATATTAAAGGCGAAAT+CGG 0.315157 8:+55147064 None:intergenic
TCCTTTCGATTCTGTTCTCT+TGG 0.334164 8:-55148198 MsG0880045173.01.T01:CDS
GTATGTGTGTGTTGTTTATT+GGG 0.334443 8:+55149898 None:intergenic
TAGTGTTTGTTTGTGTATAT+AGG 0.343461 8:-55146943 MsG0880045173.01.T01:three_prime_UTR
GCCATCTTGAAGACTTTGAT+TGG 0.367238 8:+55149823 None:intergenic
GCCCTCTTTACAAATATAGA+TGG 0.368549 8:-55146704 MsG0880045173.01.T01:three_prime_UTR
AACTATGAATTCTAGATTGT+TGG 0.377091 8:-55147506 MsG0880045173.01.T01:intron
GTCATTACCATCTTTAACTT+AGG 0.380794 8:+55146812 None:intergenic
TTCATACCATGATGTGATTA+GGG 0.385029 8:-55148373 MsG0880045173.01.T01:CDS
CAGAAAGTGTTGGACATAAC+AGG 0.395592 8:-55148339 MsG0880045173.01.T01:CDS
ACTGTTGGTGGTGGTGTTGA+AGG 0.411165 8:+55147356 None:intergenic
GCAGCACTAGTCCTCCCATT+GGG 0.414742 8:+55147288 None:intergenic
TATTGTTTGGATTGTATGAA+TGG 0.420599 8:-55148953 MsG0880045173.01.T01:CDS
TGAGTGCAATATGTATTGTT+TGG 0.429253 8:-55148966 MsG0880045173.01.T01:CDS
TGGAAGACTGAAGAACATTA+AGG 0.432710 8:-55147393 MsG0880045173.01.T01:CDS
ATCTGTGAGATACTGTAGTT+TGG 0.448774 8:-55146879 MsG0880045173.01.T01:three_prime_UTR
GTCCTGATGATGAGGAAAAC+AGG 0.450549 8:-55149066 MsG0880045173.01.T01:CDS
CTTTGGACCTAAGTTAAAGA+TGG 0.456443 8:-55146819 MsG0880045173.01.T01:three_prime_UTR
CACAGCAGCAGTTGCAGCCA+TGG 0.460151 8:-55147413 MsG0880045173.01.T01:CDS
TCACTTAGTCGAGACATTAG+TGG 0.460633 8:+55147114 None:intergenic
TAATGTTCTTCAGTCTTCCA+TGG 0.466799 8:+55147396 None:intergenic
CAGATCAGCCAAAAGAAGAA+AGG 0.467780 8:-55147327 MsG0880045173.01.T01:CDS
AAGAAACAGTTAATAATCAT+AGG 0.470126 8:-55147259 MsG0880045173.01.T01:three_prime_UTR
GTCCATCTATATTTGTAAAG+AGG 0.474739 8:+55146702 None:intergenic
CATTCACGCAGTTGGAACAT+TGG 0.478234 8:-55147181 MsG0880045173.01.T01:three_prime_UTR
TGAGGCGGTGAAAGAGGGAA+TGG 0.479527 8:+55149991 None:intergenic
GCAAGGAATCTCTAACTGTA+GGG 0.483417 8:+55147203 None:intergenic
GGTGATCATCCGATCAAGAC+TGG 0.484391 8:-55147160 MsG0880045173.01.T01:three_prime_UTR
GAATAGCACGATGATCTTTG+TGG 0.487973 8:+55148903 None:intergenic
GCTGATCTGTAATTGACTGT+TGG 0.490334 8:+55147341 None:intergenic
AGGGGAGTTCGACTCATTAA+GGG 0.493268 8:+55147222 None:intergenic
GATGGTAATGACAATAGTAT+AGG 0.493475 8:-55146801 MsG0880045173.01.T01:three_prime_UTR
TGCGGGATCCCTTGACTGAA+GGG 0.498524 8:-55147090 MsG0880045173.01.T01:three_prime_UTR
GAGGCGGTGAAAGAGGGAAT+GGG 0.500113 8:+55149992 None:intergenic
TCCATCTATATTTGTAAAGA+GGG 0.500957 8:+55146703 None:intergenic
TGCAGCACTAGTCCTCCCAT+TGG 0.504885 8:+55147287 None:intergenic
AGATCAGCCAAAAGAAGAAA+GGG 0.506864 8:-55147326 MsG0880045173.01.T01:CDS
ATTCCTCATCGTGCCCCAAT+GGG 0.509643 8:-55147302 MsG0880045173.01.T01:CDS
ATTCCTTGCATTCACGCAGT+TGG 0.509800 8:-55147189 MsG0880045173.01.T01:three_prime_UTR
CCTCAGCCAAGGCCCGGAAA+AGG 0.511324 8:-55148264 MsG0880045173.01.T01:CDS
GTGAAGTTTAGAACTATTTG+AGG 0.512491 8:+55149973 None:intergenic
AACGACAGCACTTCCCGCAC+TGG 0.520737 8:+55146968 None:intergenic
ATTCTGTTCTCTTGGCTGCA+AGG 0.521419 8:-55148190 MsG0880045173.01.T01:intron
CCTATATTATCAACAGTGCA+AGG 0.522886 8:-55148308 MsG0880045173.01.T01:CDS
GAGAGGCCTCAGCCAAGGCC+CGG 0.529902 8:-55148270 MsG0880045173.01.T01:CDS
GTAATGACAATAGTATAGGT+TGG 0.530750 8:-55146797 MsG0880045173.01.T01:three_prime_UTR
ACTATTTGAGGCGGTGAAAG+AGG 0.531396 8:+55149985 None:intergenic
AATTCCTCATCGTGCCCCAA+TGG 0.532814 8:-55147303 MsG0880045173.01.T01:CDS
GATCTGTAATTGACTGTTGG+TGG 0.533120 8:+55147344 None:intergenic
GTTCCAACTGCGTGAATGCA+AGG 0.534287 8:+55147186 None:intergenic
CTGTAATTGACTGTTGGTGG+TGG 0.534739 8:+55147347 None:intergenic
ATCGGATTCCCTTCAGTCAA+GGG 0.544915 8:+55147082 None:intergenic
TTGTGATGAATGTGAGAAGT+GGG 0.553224 8:+55149868 None:intergenic
CTCGCAGACTTCACAAGTGT+TGG 0.560178 8:+55148236 None:intergenic
TGCAAGGAATCTCTAACTGT+AGG 0.563310 8:+55147202 None:intergenic
CATTCACCCTAATCACATCA+TGG 0.571167 8:+55148367 None:intergenic
CTATTTGAGGCGGTGAAAGA+GGG 0.572562 8:+55149986 None:intergenic
TTGGTCTATCCAGTCTTGAT+CGG 0.575600 8:+55147151 None:intergenic
AATCGGATTCCCTTCAGTCA+AGG 0.575945 8:+55147081 None:intergenic
GGGTTGTGTTCTTGAATGAG+AGG 0.577373 8:-55148287 MsG0880045173.01.T01:CDS
TCCAATCAAAGTCTTCAAGA+TGG 0.579850 8:-55149824 MsG0880045173.01.T01:exon
TTGAGCAGAGGTTTCAACCA+TGG 0.583253 8:+55149037 None:intergenic
AAGAAACGCTCTTTGAGCAG+AGG 0.583635 8:+55149025 None:intergenic
GAATGAGATTCAGAAAGTGT+TGG 0.585916 8:-55148349 MsG0880045173.01.T01:CDS
TTGGTGAAAAGGGCCAGTGC+GGG 0.588645 8:-55146981 MsG0880045173.01.T01:three_prime_UTR
ATGCGGGATCCCTTGACTGA+AGG 0.599792 8:-55147091 MsG0880045173.01.T01:three_prime_UTR
TGTCTCGACTAAGTGAATGC+GGG 0.600588 8:-55147107 MsG0880045173.01.T01:three_prime_UTR
AGGAAAACAGGTGGCCACCA+TGG 0.603560 8:-55149054 MsG0880045173.01.T01:CDS
CCTCATCGTGCCCCAATGGG+AGG 0.612946 8:-55147299 MsG0880045173.01.T01:CDS
ATGTCTCGACTAAGTGAATG+CGG 0.613597 8:-55147108 MsG0880045173.01.T01:three_prime_UTR
CCTCCCATTGGGGCACGATG+AGG 0.616154 8:+55147299 None:intergenic
TGAATGAGAGGCCTCAGCCA+AGG 0.624070 8:-55148275 MsG0880045173.01.T01:CDS
TGTTTGATATATAGATAAGG+AGG 0.625044 8:-55148398 MsG0880045173.01.T01:intron
GCCAAGAGAACAGAATCGAA+AGG 0.631094 8:+55148197 None:intergenic
AGCAGAGGTTTCAACCATGG+TGG 0.633067 8:+55149040 None:intergenic
AGAATCGAAAGGAATCGAGA+AGG 0.635045 8:+55148208 None:intergenic
CAAGGAATCTCTAACTGTAG+GGG 0.637324 8:+55147204 None:intergenic
TGCTGGAGGTCCTGATGATG+AGG 0.642425 8:-55149074 MsG0880045173.01.T01:CDS
AAGTTTAGAACTATTTGAGG+CGG 0.644270 8:+55149976 None:intergenic
CAGCACTAGTCCTCCCATTG+GGG 0.647798 8:+55147289 None:intergenic
TTCAATGATATCAGACTCAG+AGG 0.648076 8:-55147441 MsG0880045173.01.T01:CDS
TTTGGTGAAAAGGGCCAGTG+CGG 0.648558 8:-55146982 MsG0880045173.01.T01:three_prime_UTR
CTATATTATCAACAGTGCAA+GGG 0.657853 8:-55148307 MsG0880045173.01.T01:CDS
TTTGTGATGAATGTGAGAAG+TGG 0.667949 8:+55149867 None:intergenic
CTGATGATGAGGAAAACAGG+TGG 0.717367 8:-55149063 MsG0880045173.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTGATAATTTTTTTGAA+GGG - Chr8:55147637-55147656 MsG0880045173.01.T01:intron 10.0%
!!! ATTATTATTACTACTATTTT+GGG + Chr8:55148629-55148648 None:intergenic 10.0%
!!! CTATTTAAAATATTGATCAA+TGG - Chr8:55148948-55148967 MsG0880045173.01.T01:CDS 15.0%
!!! GAATTTGATAATTTTTTTGA+AGG - Chr8:55147636-55147655 MsG0880045173.01.T01:intron 15.0%
!!! GATTATTATTACTACTATTT+TGG + Chr8:55148630-55148649 None:intergenic 15.0%
!!! TAAACTAGTGTTAATTTTTT+TGG - Chr8:55147100-55147119 MsG0880045173.01.T01:three_prime_UTR 15.0%
!!! TTAATTTTTTGTTTTGTTTC+TGG - Chr8:55147267-55147286 MsG0880045173.01.T01:exon 15.0%
!!! TTGATCAATATTTTAAATAG+TGG + Chr8:55148948-55148967 None:intergenic 15.0%
!!! TTTTTTCGTATCTATATTAA+AGG + Chr8:55149682-55149701 None:intergenic 15.0%
!! AAATTGAATTGAATGCAAAA+GGG + Chr8:55149133-55149152 None:intergenic 20.0%
!! AAGAAACAGTTAATAATCAT+AGG - Chr8:55149475-55149494 MsG0880045173.01.T01:intron 20.0%
!! AATAAAAAGAGAGAAAAATG+TGG + Chr8:55146812-55146831 None:intergenic 20.0%
!! ATAATCATGAATGCTAATAT+AGG - Chr8:55149042-55149061 MsG0880045173.01.T01:CDS 20.0%
!! TAAAAATTGAAAACAGCAAA+AGG + Chr8:55148006-55148025 None:intergenic 20.0%
!! TAAATTGAATTGAATGCAAA+AGG + Chr8:55149134-55149153 None:intergenic 20.0%
!! TATAGATACGAAAAAATCTA+AGG - Chr8:55149686-55149705 MsG0880045173.01.T01:intron 20.0%
!! TATTATAGACTATTCTTATG+AGG + Chr8:55148813-55148832 None:intergenic 20.0%
!! TTGAAATGACAAAATTTCAA+AGG + Chr8:55149104-55149123 None:intergenic 20.0%
!!! AGTAAGTTATATGTAAGTTT+TGG - Chr8:55149968-55149987 MsG0880045173.01.T01:five_prime_UTR 20.0%
!!! ATTTGTTTTGATTGCAATTT+TGG - Chr8:55148734-55148753 MsG0880045173.01.T01:intron 20.0%
!!! CAATATTTTAAATAGTGGAT+TGG + Chr8:55148943-55148962 None:intergenic 20.0%
!!! GAAATTTTGTCATTTCAATA+TGG - Chr8:55149106-55149125 MsG0880045173.01.T01:intron 20.0%
!!! GTAAGTTATATGTAAGTTTT+GGG - Chr8:55149969-55149988 MsG0880045173.01.T01:five_prime_UTR 20.0%
!!! TAAGTTATATGTAAGTTTTG+GGG - Chr8:55149970-55149989 MsG0880045173.01.T01:five_prime_UTR 20.0%
!!! TAATTGTGTTTTTGAATTCT+GGG - Chr8:55147310-55147329 MsG0880045173.01.T01:CDS 20.0%
!!! TATTTGTGATTTTGAATTGT+GGG - Chr8:55147604-55147623 MsG0880045173.01.T01:intron 20.0%
!!! TATTTGTGTTTTTGAATTCT+GGG - Chr8:55147460-55147479 MsG0880045173.01.T01:CDS 20.0%
!!! TCTTGTATTTTAGAGAATTA+CGG + Chr8:55148848-55148867 None:intergenic 20.0%
!!! TTAATTGTGTTTTTGAATTC+TGG - Chr8:55147309-55147328 MsG0880045173.01.T01:CDS 20.0%
!!! TTATTTGTGATTTTGAATTG+TGG - Chr8:55147603-55147622 MsG0880045173.01.T01:intron 20.0%
!!! TTATTTGTGTTTTTGAATTC+TGG - Chr8:55147459-55147478 MsG0880045173.01.T01:CDS 20.0%
!!! TTTTTTGAAATGCAATGATT+TGG - Chr8:55147577-55147596 MsG0880045173.01.T01:intron 20.0%
! AAACAAGCAAAAAGTTTCTT+TGG - Chr8:55149898-55149917 MsG0880045173.01.T01:five_prime_UTR 25.0%
! AAATAATGAACACACAATGA+AGG + Chr8:55147223-55147242 None:intergenic 25.0%
! AACACAATAAATGTGTCAAA+GGG + Chr8:55147936-55147955 None:intergenic 25.0%
! AACTATGAATTCTAGATTGT+TGG - Chr8:55149228-55149247 MsG0880045173.01.T01:intron 25.0%
! AATGACAAAATTTCAAAGGT+TGG + Chr8:55149100-55149119 None:intergenic 25.0%
! AATGGGAATTTATGGATAAT+TGG + Chr8:55148034-55148053 None:intergenic 25.0%
! ATCTATATTAAAGGCGAAAT+CGG + Chr8:55149673-55149692 None:intergenic 25.0%
! ATCTTATTCAAATGGTTAGA+AGG + Chr8:55148601-55148620 None:intergenic 25.0%
! ATGACAAAATTTCAAAGGTT+GGG + Chr8:55149099-55149118 None:intergenic 25.0%
! CACAACTTTGATGAATTTAA+TGG + Chr8:55147877-55147896 None:intergenic 25.0%
! CATCAAAATCCTTGATTTAA+CGG + Chr8:55148670-55148689 None:intergenic 25.0%
! GAACAATAAATGGGAATTTA+TGG + Chr8:55148042-55148061 None:intergenic 25.0%
! GAATTAATTGTAGTTACAAG+TGG + Chr8:55148163-55148182 None:intergenic 25.0%
! GTGTGTTTGATATATAGATA+AGG - Chr8:55148333-55148352 MsG0880045173.01.T01:CDS 25.0%
! TAGTGTTTGTTTGTGTATAT+AGG - Chr8:55149791-55149810 MsG0880045173.01.T01:CDS 25.0%
! TATTGTTTGGATTGTATGAA+TGG - Chr8:55147781-55147800 MsG0880045173.01.T01:intron 25.0%
! TCATTGTGTGTTCATTATTT+GGG - Chr8:55147223-55147242 MsG0880045173.01.T01:three_prime_UTR 25.0%
! TCCATCTATATTTGTAAAGA+GGG + Chr8:55150034-55150053 None:intergenic 25.0%
! TCTTATTCAAATGGTTAGAA+GGG + Chr8:55148600-55148619 None:intergenic 25.0%
! TGTAGTTTGGTAAAAAGAAA+TGG - Chr8:55149868-55149887 MsG0880045173.01.T01:five_prime_UTR 25.0%
! TGTTTGATATATAGATAAGG+AGG - Chr8:55148336-55148355 MsG0880045173.01.T01:CDS 25.0%
!! GTTTTTCTGACAATAATAAC+AGG + Chr8:55147959-55147978 None:intergenic 25.0%
!!! TGTTTGTGTTTTTGAATTCT+GGG - Chr8:55147144-55147163 MsG0880045173.01.T01:three_prime_UTR 25.0%
!!! TTCTGGAATGTAATGTTTTT+GGG - Chr8:55147284-55147303 MsG0880045173.01.T01:CDS 25.0%
!!! TTGAAGAAAGAGTTTTGTTT+TGG + Chr8:55146997-55147016 None:intergenic 25.0%
!!! TTGTTTGTGTTTTTGAATTC+TGG - Chr8:55147143-55147162 MsG0880045173.01.T01:three_prime_UTR 25.0%
!!! TTTCTGGAATGTAATGTTTT+TGG - Chr8:55147283-55147302 MsG0880045173.01.T01:CDS 25.0%
AAATTTCAAAGGTTGGGTAT+AGG + Chr8:55149093-55149112 None:intergenic 30.0%
AGTGGTTGTTCAATCAAAAT+TGG + Chr8:55149605-55149624 None:intergenic 30.0%
CTATATTATCAACAGTGCAA+GGG - Chr8:55148427-55148446 MsG0880045173.01.T01:intron 30.0%
CTCATTGTGTGTTCATTATT+TGG - Chr8:55147370-55147389 MsG0880045173.01.T01:CDS 30.0%
CTTATTCAAATGGTTAGAAG+GGG + Chr8:55148599-55148618 None:intergenic 30.0%
GAACACAATAAATGTGTCAA+AGG + Chr8:55147937-55147956 None:intergenic 30.0%
GGGAGTTAATCTTATTCAAA+TGG + Chr8:55148609-55148628 None:intergenic 30.0%
GGTAGTTACGAACAATAAAT+GGG + Chr8:55148051-55148070 None:intergenic 30.0%
GTAATGACAATAGTATAGGT+TGG - Chr8:55149937-55149956 MsG0880045173.01.T01:five_prime_UTR 30.0%
GTATATAGGAAAAATCCAAC+AGG - Chr8:55149805-55149824 MsG0880045173.01.T01:CDS 30.0%
GTCCATCTATATTTGTAAAG+AGG + Chr8:55150035-55150054 None:intergenic 30.0%
TCATTATTTGGGAGTTAACT+TGG - Chr8:55147525-55147544 MsG0880045173.01.T01:intron 30.0%
TGAGTGCAATATGTATTGTT+TGG - Chr8:55147768-55147787 MsG0880045173.01.T01:intron 30.0%
TGGTAGTTACGAACAATAAA+TGG + Chr8:55148052-55148071 None:intergenic 30.0%
TTCATACCATGATGTGATTA+GGG - Chr8:55148361-55148380 MsG0880045173.01.T01:CDS 30.0%
TTGGTAAAAAGAAATGGTGT+AGG - Chr8:55149874-55149893 MsG0880045173.01.T01:five_prime_UTR 30.0%
! AAGTTTAGAACTATTTGAGG+CGG + Chr8:55146761-55146780 None:intergenic 30.0%
! AGCTTTTCAATGCAATAACA+TGG - Chr8:55148281-55148300 MsG0880045173.01.T01:CDS 30.0%
! ATTGGGAATGCAATGATTTT+AGG - Chr8:55147434-55147453 MsG0880045173.01.T01:CDS 30.0%
! GATGGTAATGACAATAGTAT+AGG - Chr8:55149933-55149952 MsG0880045173.01.T01:five_prime_UTR 30.0%
! GTCATTACCATCTTTAACTT+AGG + Chr8:55149925-55149944 None:intergenic 30.0%
! GTGAAGTTTAGAACTATTTG+AGG + Chr8:55146764-55146783 None:intergenic 30.0%
!! AAAAACCATCACTTTTCCTT+TGG - Chr8:55147973-55147992 MsG0880045173.01.T01:intron 30.0%
!! ATTTTGTAGAACTTTGAAGG+CGG + Chr8:55146722-55146741 None:intergenic 30.0%
!! GTATGTGTGTGTTGTTTATT+GGG + Chr8:55146839-55146858 None:intergenic 30.0%
!! TGTATGTGTGTGTTGTTTAT+TGG + Chr8:55146840-55146859 None:intergenic 30.0%
!!! ATAATTTTTTTGAAGGGTGC+TGG - Chr8:55147643-55147662 MsG0880045173.01.T01:intron 30.0%
!!! TGTTTTGACTTTTGAGTCTA+TGG - Chr8:55149185-55149204 MsG0880045173.01.T01:intron 30.0%
!!! TGTTTTTGGGCTTAATTTCA+CGG + Chr8:55146958-55146977 None:intergenic 30.0%
ACACCAACAACAAAATGTGT+CGG + Chr8:55148111-55148130 None:intergenic 35.0%
ACCTCAATGCATTAACATTG+AGG + Chr8:55148772-55148791 None:intergenic 35.0%
ACCTCAATGTTAATGCATTG+AGG - Chr8:55148768-55148787 MsG0880045173.01.T01:intron 35.0%
AGATCAGCCAAAAGAAGAAA+GGG - Chr8:55149408-55149427 MsG0880045173.01.T01:intron 35.0%
ATCTGTGAGATACTGTAGTT+TGG - Chr8:55149855-55149874 MsG0880045173.01.T01:five_prime_UTR 35.0%
CAAATCCAAAGGAAAAGTGA+TGG + Chr8:55147981-55148000 None:intergenic 35.0%
CCTATATTATCAACAGTGCA+AGG - Chr8:55148426-55148445 MsG0880045173.01.T01:intron 35.0%
CTTCATACCATGATGTGATT+AGG - Chr8:55148360-55148379 MsG0880045173.01.T01:CDS 35.0%
CTTTGGACCTAAGTTAAAGA+TGG - Chr8:55149915-55149934 MsG0880045173.01.T01:five_prime_UTR 35.0%
GCCCTCTTTACAAATATAGA+TGG - Chr8:55150030-55150049 MsG0880045173.01.T01:five_prime_UTR 35.0%
GTGAAATTAAGCCCAAAAAC+AGG - Chr8:55146957-55146976 MsG0880045173.01.T01:three_prime_UTR 35.0%
TAATGTTCTTCAGTCTTCCA+TGG + Chr8:55149341-55149360 None:intergenic 35.0%
TCAAGCTAGCACTTAAAAGT+GGG - Chr8:55149007-55149026 MsG0880045173.01.T01:CDS 35.0%
TCCAATCAAAGTCTTCAAGA+TGG - Chr8:55146910-55146929 MsG0880045173.01.T01:three_prime_UTR 35.0%
TGATTTAACGGCCAAAAGTT+TGG + Chr8:55148658-55148677 None:intergenic 35.0%
TGGAAGACTGAAGAACATTA+AGG - Chr8:55149341-55149360 MsG0880045173.01.T01:intron 35.0%
TTCAAGCTAGCACTTAAAAG+TGG - Chr8:55149006-55149025 MsG0880045173.01.T01:CDS 35.0%
TTCAATGATATCAGACTCAG+AGG - Chr8:55149293-55149312 MsG0880045173.01.T01:intron 35.0%
TTGTGATGAATGTGAGAAGT+GGG + Chr8:55146869-55146888 None:intergenic 35.0%
TTTGTGATGAATGTGAGAAG+TGG + Chr8:55146870-55146889 None:intergenic 35.0%
! AACTTTTGGCCGTTAAATCA+AGG - Chr8:55148658-55148677 MsG0880045173.01.T01:intron 35.0%
! AGGAATTCCCTTTCTTCTTT+TGG + Chr8:55149418-55149437 None:intergenic 35.0%
! ATAATCCGCTACCAAACTTT+TGG - Chr8:55148644-55148663 MsG0880045173.01.T01:intron 35.0%
! GAATGAGATTCAGAAAGTGT+TGG - Chr8:55148385-55148404 MsG0880045173.01.T01:CDS 35.0%
! GGGATTTTGTAGAACTTTGA+AGG + Chr8:55146725-55146744 None:intergenic 35.0%
! TACCATGATTCTCCTGTTTT+TGG + Chr8:55146972-55146991 None:intergenic 35.0%
!! ACCATGATTCTCCTGTTTTT+GGG + Chr8:55146971-55146990 None:intergenic 35.0%
!! CATCACTTTTCCTTTGGATT+TGG - Chr8:55147979-55147998 MsG0880045173.01.T01:intron 35.0%
!! CCTTGCACTGTTGATAATAT+AGG + Chr8:55148429-55148448 None:intergenic 35.0%
!! CTAGAGCTTTTTGGTGAAAA+GGG - Chr8:55149743-55149762 MsG0880045173.01.T01:intron 35.0%
!!! AGTTTTGGGGATGTAAATAG+TGG - Chr8:55149983-55150002 MsG0880045173.01.T01:five_prime_UTR 35.0%
AAGGGGAAGACATTATGTGA+AGG + Chr8:55148582-55148601 None:intergenic 40.0%
AGAATCGAAAGGAATCGAGA+AGG + Chr8:55148529-55148548 None:intergenic 40.0%
AGCAAAAGGACCAAATCCAA+AGG + Chr8:55147992-55148011 None:intergenic 40.0%
ATGTCTCGACTAAGTGAATG+CGG - Chr8:55149626-55149645 MsG0880045173.01.T01:intron 40.0%
CAACAGGAGTGGAAAAAGTT+AGG - Chr8:55149821-55149840 MsG0880045173.01.T01:exon 40.0%
CAAGGAATCTCTAACTGTAG+GGG + Chr8:55149533-55149552 None:intergenic 40.0%
CAATCTAAACCATCATTGCG+TGG + Chr8:55148072-55148091 None:intergenic 40.0%
CAGACACCAACAACAAATGT+CGG + Chr8:55148140-55148159 None:intergenic 40.0%
CAGATCAGCCAAAAGAAGAA+AGG - Chr8:55149407-55149426 MsG0880045173.01.T01:intron 40.0%
CATTCACCCTAATCACATCA+TGG + Chr8:55148370-55148389 None:intergenic 40.0%
GAATAGCACGATGATCTTTG+TGG + Chr8:55147834-55147853 None:intergenic 40.0%
GATCTGTAATTGACTGTTGG+TGG + Chr8:55149393-55149412 None:intergenic 40.0%
GCAAGGAATCTCTAACTGTA+GGG + Chr8:55149534-55149553 None:intergenic 40.0%
GCCATCTTGAAGACTTTGAT+TGG + Chr8:55146914-55146933 None:intergenic 40.0%
GCTGATCTGTAATTGACTGT+TGG + Chr8:55149396-55149415 None:intergenic 40.0%
TAGGAAAAATCCAACAGGAG+TGG - Chr8:55149810-55149829 MsG0880045173.01.T01:exon 40.0%
TCACTTAGTCGAGACATTAG+TGG + Chr8:55149623-55149642 None:intergenic 40.0%
TCCTTTCGATTCTGTTCTCT+TGG - Chr8:55148536-55148555 MsG0880045173.01.T01:intron 40.0%
TGCAAGGAATCTCTAACTGT+AGG + Chr8:55149535-55149554 None:intergenic 40.0%
TTAGCTCACACTCTCATTGT+TGG - Chr8:55147052-55147071 MsG0880045173.01.T01:three_prime_UTR 40.0%
TTGGTCTATCCAGTCTTGAT+CGG + Chr8:55149586-55149605 None:intergenic 40.0%
! CAAAGATCATCGTGCTATTC+AGG - Chr8:55147834-55147853 MsG0880045173.01.T01:intron 40.0%
! GTTAGTGTCATTGTTGTGTC+TGG - Chr8:55148216-55148235 MsG0880045173.01.T01:CDS 40.0%
!! CAGAAAGTGTTGGACATAAC+AGG - Chr8:55148395-55148414 MsG0880045173.01.T01:intron 40.0%
!! CTAACTTTTTCCACTCCTGT+TGG + Chr8:55149823-55149842 None:intergenic 40.0%
!! GACTTTTGAGTCTATGGCAT+TGG - Chr8:55149191-55149210 MsG0880045173.01.T01:intron 40.0%
!! GCTAGAGCTTTTTGGTGAAA+AGG - Chr8:55149742-55149761 MsG0880045173.01.T01:intron 40.0%
!! TGTCCGACACATTTTGTTGT+TGG - Chr8:55148105-55148124 MsG0880045173.01.T01:intron 40.0%
!!! ATTTTTTTGAAGGGTGCTGG+AGG - Chr8:55147646-55147665 MsG0880045173.01.T01:intron 40.0%
AACGGCCAAAAGTTTGGTAG+CGG + Chr8:55148652-55148671 None:intergenic 45.0%
AAGAAACGCTCTTTGAGCAG+AGG + Chr8:55147712-55147731 None:intergenic 45.0%
AATCGGATTCCCTTCAGTCA+AGG + Chr8:55149656-55149675 None:intergenic 45.0%
AGGGGAGTTCGACTCATTAA+GGG + Chr8:55149515-55149534 None:intergenic 45.0%
ATCGGATTCCCTTCAGTCAA+GGG + Chr8:55149655-55149674 None:intergenic 45.0%
ATGCGACACTGAACTAGACA+CGG + Chr8:55148245-55148264 None:intergenic 45.0%
ATTCCTTGCATTCACGCAGT+TGG - Chr8:55149545-55149564 MsG0880045173.01.T01:intron 45.0%
ATTCTGTTCTCTTGGCTGCA+AGG - Chr8:55148544-55148563 MsG0880045173.01.T01:intron 45.0%
CATTCACGCAGTTGGAACAT+TGG - Chr8:55149553-55149572 MsG0880045173.01.T01:intron 45.0%
GCCAAGAGAACAGAATCGAA+AGG + Chr8:55148540-55148559 None:intergenic 45.0%
GCCCAAAAACAGGAGAATCA+TGG - Chr8:55146967-55146986 MsG0880045173.01.T01:three_prime_UTR 45.0%
GGGTTGTGTTCTTGAATGAG+AGG - Chr8:55148447-55148466 MsG0880045173.01.T01:intron 45.0%
TAGGGGAGTTCGACTCATTA+AGG + Chr8:55149516-55149535 None:intergenic 45.0%
TCGTAACTACCACGCAATGA+TGG - Chr8:55148060-55148079 MsG0880045173.01.T01:intron 45.0%
TGTCTCGACTAAGTGAATGC+GGG - Chr8:55149627-55149646 MsG0880045173.01.T01:intron 45.0%
TTGAGCAGAGGTTTCAACCA+TGG + Chr8:55147700-55147719 None:intergenic 45.0%
! ACTATTTGAGGCGGTGAAAG+AGG + Chr8:55146752-55146771 None:intergenic 45.0%
! CACCTGTTTTCCTCATCATC+AGG + Chr8:55147673-55147692 None:intergenic 45.0%
! CTATTTGAGGCGGTGAAAGA+GGG + Chr8:55146751-55146770 None:intergenic 45.0%
! CTGATGATGAGGAAAACAGG+TGG - Chr8:55147671-55147690 MsG0880045173.01.T01:intron 45.0%
! CTGTAATTGACTGTTGGTGG+TGG + Chr8:55149390-55149409 None:intergenic 45.0%
! GTCCTGATGATGAGGAAAAC+AGG - Chr8:55147668-55147687 MsG0880045173.01.T01:intron 45.0%
! GTTCCAGTGCTAGAGCTTTT+TGG - Chr8:55149734-55149753 MsG0880045173.01.T01:intron 45.0%
! TCACCAAAAAGCTCTAGCAC+TGG + Chr8:55149740-55149759 None:intergenic 45.0%
!! CAGACACCGACATTTGTTGT+TGG - Chr8:55148131-55148150 MsG0880045173.01.T01:intron 45.0%
!! GAGCTGACTTGTTCTTCTTC+TGG + Chr8:55149269-55149288 None:intergenic 45.0%
!!! AGTGTTGGTGACACCTTTTC+CGG + Chr8:55148486-55148505 None:intergenic 45.0%
!!! AATTTTTTTGTAAAAAAATT+GGG - Chr8:55147417-55147436 MsG0880045173.01.T01:CDS 5.0%
!!! TAATTTTTTTGTAAAAAAAT+TGG - Chr8:55147416-55147435 MsG0880045173.01.T01:CDS 5.0%
AATTCCTCATCGTGCCCCAA+TGG - Chr8:55149431-55149450 MsG0880045173.01.T01:intron 50.0%
AGCAGAGGTTTCAACCATGG+TGG + Chr8:55147697-55147716 None:intergenic 50.0%
ATTCCTCATCGTGCCCCAAT+GGG - Chr8:55149432-55149451 MsG0880045173.01.T01:intron 50.0%
CTCGCAGACTTCACAAGTGT+TGG + Chr8:55148501-55148520 None:intergenic 50.0%
GGTGATCATCCGATCAAGAC+TGG - Chr8:55149574-55149593 MsG0880045173.01.T01:intron 50.0%
GTTCCAACTGCGTGAATGCA+AGG + Chr8:55149551-55149570 None:intergenic 50.0%
TTTGGTGAAAAGGGCCAGTG+CGG - Chr8:55149752-55149771 MsG0880045173.01.T01:intron 50.0%
!! ACTGTTGGTGGTGGTGTTGA+AGG + Chr8:55149381-55149400 None:intergenic 50.0%
!!! GTGTTGGTGACACCTTTTCC+GGG + Chr8:55148485-55148504 None:intergenic 50.0%
AGGAAAACAGGTGGCCACCA+TGG - Chr8:55147680-55147699 MsG0880045173.01.T01:intron 55.0%
ATGCGGGATCCCTTGACTGA+AGG - Chr8:55149643-55149662 MsG0880045173.01.T01:intron 55.0%
CAGCACTAGTCCTCCCATTG+GGG + Chr8:55149448-55149467 None:intergenic 55.0%
GAGGCGGTGAAAGAGGGAAT+GGG + Chr8:55146745-55146764 None:intergenic 55.0%
GCAGCACTAGTCCTCCCATT+GGG + Chr8:55149449-55149468 None:intergenic 55.0%
TGAATGAGAGGCCTCAGCCA+AGG - Chr8:55148459-55148478 MsG0880045173.01.T01:intron 55.0%
TGAGGCGGTGAAAGAGGGAA+TGG + Chr8:55146746-55146765 None:intergenic 55.0%
TGCAGCACTAGTCCTCCCAT+TGG + Chr8:55149450-55149469 None:intergenic 55.0%
TGCGGGATCCCTTGACTGAA+GGG - Chr8:55149644-55149663 MsG0880045173.01.T01:intron 55.0%
TTGGTGAAAAGGGCCAGTGC+GGG - Chr8:55149753-55149772 MsG0880045173.01.T01:intron 55.0%
! TGCTGGAGGTCCTGATGATG+AGG - Chr8:55147660-55147679 MsG0880045173.01.T01:intron 55.0%
AACGACAGCACTTCCCGCAC+TGG + Chr8:55149769-55149788 None:intergenic 60.0%
CACAGCAGCAGTTGCAGCCA+TGG - Chr8:55149321-55149340 MsG0880045173.01.T01:intron 60.0%
! GTGACACCTTTTCCGGGCCT+TGG + Chr8:55148479-55148498 None:intergenic 60.0%
CCTCAGCCAAGGCCCGGAAA+AGG - Chr8:55148470-55148489 MsG0880045173.01.T01:intron 65.0%
CCTCATCGTGCCCCAATGGG+AGG - Chr8:55149435-55149454 MsG0880045173.01.T01:intron 65.0%
CCTCCCATTGGGGCACGATG+AGG + Chr8:55149438-55149457 None:intergenic 65.0%
! CCTTTTCCGGGCCTTGGCTG+AGG + Chr8:55148473-55148492 None:intergenic 65.0%
GAGAGGCCTCAGCCAAGGCC+CGG - Chr8:55148464-55148483 MsG0880045173.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
Chr8 gene 55146659 55150097 55146659 ID=MsG0880045173.01;Name=MsG0880045173.01
Chr8 mRNA 55146659 55150097 55146659 ID=MsG0880045173.01.T01;Parent=MsG0880045173.01;Name=MsG0880045173.01.T01;_AED=0.27;_eAED=0.27;_QI=270|0.66|0.75|1|1|1|4|621|242
Chr8 exon 55146659 55147513 55146659 ID=MsG0880045173.01.T01:exon:23811;Parent=MsG0880045173.01.T01
Chr8 exon 55148191 55148406 55148191 ID=MsG0880045173.01.T01:exon:23810;Parent=MsG0880045173.01.T01
Chr8 exon 55148901 55149119 55148901 ID=MsG0880045173.01.T01:exon:23809;Parent=MsG0880045173.01.T01
Chr8 exon 55149768 55150097 55149768 ID=MsG0880045173.01.T01:exon:23808;Parent=MsG0880045173.01.T01
Chr8 five_prime_UTR 55149828 55150097 55149828 ID=MsG0880045173.01.T01:five_prime_utr;Parent=MsG0880045173.01.T01
Chr8 CDS 55149768 55149827 55149768 ID=MsG0880045173.01.T01:cds;Parent=MsG0880045173.01.T01
Chr8 CDS 55148901 55149119 55148901 ID=MsG0880045173.01.T01:cds;Parent=MsG0880045173.01.T01
Chr8 CDS 55148191 55148406 55148191 ID=MsG0880045173.01.T01:cds;Parent=MsG0880045173.01.T01
Chr8 CDS 55147280 55147513 55147280 ID=MsG0880045173.01.T01:cds;Parent=MsG0880045173.01.T01
Chr8 three_prime_UTR 55146659 55147279 55146659 ID=MsG0880045173.01.T01:three_prime_utr;Parent=MsG0880045173.01.T01
Gene Sequence

>MsG0880045173.01.T01

ATGGCGTTTGAAAATCAAGACACTGTCCGTGAAATTAAGCCCAAAAACAGGAGAATCATGAATTTGATAATTTTTTTGAAGGGTGCTGGAGGTCCTGATGATGAGGAAAACAGGTGGCCACCATGGTTGAAACCTCTGCTCAAAGAGCGTTTCTTTGTTCAATGCAAGTTGCATGCTGATTCACACAAGAGTGAGTGCAATATGTATTGTTTGGATTGTATGAATGGTGCTCTTTGCTCACTCTGTCTCAATTACCACAAAGATCATCGTGCTATTCAGATAAGGAGGTCTTCATACCATGATGTGATTAGGGTGAATGAGATTCAGAAAGTGTTGGACATAACAGGTGTTCAAACCTATATTATCAACAGTGCAAGGGTTGTGTTCTTGAATGAGAGGCCTCAGCCAAGGCCCGGAAAAGGTGTCACCAACACTTGTGAAGTCTGCGAGCGTAGCCTTCTCGATTCCTTTCGATTCTGTTCTCTTGGCTGCAAGATTGTTGGAACTTCAATGAATTTCCAGAAGAAGAACAAGTCAGCTCGTGCTTCAATGATATCAGACTCAGAGGATTCACACAGCAGCAGTTGCAGCCATGGAAGACTGAAGAACATTAAGGTGCAAAGCTTCACTCCTTCAACACCACCACCAACAGTCAATTACAGATCAGCCAAAAGAAGAAAGGGAATTCCTCATCGTGCCCCAATGGGAGGACTAGTGCTGCAATACTAA
Protein sequence

>MsG0880045173.01.T01

MAFENQDTVREIKPKNRRIMNLIIFLKGAGGPDDEENRWPPWLKPLLKERFFVQCKLHADSHKSECNMYCLDCMNGALCSLCLNYHKDHRAIQIRRSSYHDVIRVNEIQKVLDITGVQTYIINSARVVFLNERPQPRPGKGVTNTCEVCERSLLDSFRFCSLGCKIVGTSMNFQKKNKSARASMISDSEDSHSSSCSHGRLKNIKVQSFTPSTPPPTVNYRSAKRRKGIPHRAPMGGLVLQY*