AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040424.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040424.01.T01 MTR_7g090210 84.138 145 2 1 1 145 1 124 4.83e-84 242
MsG0780040424.01.T01 MTR_7g090210 82.270 141 4 1 1 141 1 120 6.72e-81 236
MsG0780040424.01.T01 MTR_5g090010 50.704 142 48 2 1 141 4 124 1.21e-44 144
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040424.01.T01 AT3G57090 55.000 140 42 1 1 140 1 119 5.58e-51 160
MsG0780040424.01.T01 AT3G57090 55.000 140 42 1 1 140 1 119 5.58e-51 160
MsG0780040424.01.T01 AT5G12390 48.276 145 46 3 1 140 1 121 1.60e-39 131

Find 41 sgRNAs with CRISPR-Local

Find 113 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTAGGAGTCGGCAGCTTC+TGG 0.252357 7:-77380378 MsG0780040424.01.T01:CDS
ATTATGATTCGCAGCTTCTT+TGG 0.302296 7:-77380477 MsG0780040424.01.T01:intron
GTAACTTTCCGTTGAGTTGC+AGG 0.337755 7:-77381907 MsG0780040424.01.T01:five_prime_UTR
TTCTTGCTGTTGGGTACTAC+AGG 0.348000 7:-77380412 MsG0780040424.01.T01:CDS
AGCTTTATCTTCTTGCTGTT+GGG 0.356980 7:-77380421 MsG0780040424.01.T01:CDS
GCAGCTTCTGGAGCAATGTT+TGG 0.361994 7:-77380366 MsG0780040424.01.T01:CDS
TTATGATTCGCAGCTTCTTT+GGG 0.372741 7:-77380476 MsG0780040424.01.T01:intron
CATGGGCACTTGTTCATTCA+AGG 0.399932 7:-77381349 MsG0780040424.01.T01:CDS
AAGCTTTATCTTCTTGCTGT+TGG 0.416064 7:-77380422 MsG0780040424.01.T01:CDS
AGCTTCTCTCTTTGATGTAA+AGG 0.431754 7:+77380440 None:intergenic
TCGTCGATGGAGGCTAAAAT+CGG 0.445374 7:-77381870 MsG0780040424.01.T01:exon
GCTTCTGGAGCAATGTTTGG+AGG 0.445797 7:-77380363 MsG0780040424.01.T01:CDS
TCCCTCGGAAACTTCTTTAC+CGG 0.457316 7:-77381834 MsG0780040424.01.T01:CDS
TGAATTGCAAATACACTGTT+TGG 0.463003 7:-77381483 MsG0780040424.01.T01:CDS
GAAGTTGCAGAGGCTTCTAA+TGG 0.471526 7:-77381413 MsG0780040424.01.T01:CDS
GGAAGCTTCTTTGAATCCCT+CGG 0.481996 7:-77381849 MsG0780040424.01.T01:CDS
TCCAGAAGCTGCCGACTCCT+AGG 0.515588 7:+77380377 None:intergenic
TGATAGTTAACCGTCGTCGA+TGG 0.516268 7:-77381883 MsG0780040424.01.T01:exon
AGTAATTATACTTTCGAGCA+TGG 0.516555 7:+77381298 None:intergenic
CCGGCGGCGATCAGATCCCT+TGG 0.523323 7:-77381815 MsG0780040424.01.T01:CDS
AGAGCATAATGAGGTTGTCA+TGG 0.535428 7:-77381367 MsG0780040424.01.T01:CDS
ACAGGGCCACTGATTATCCT+AGG 0.539913 7:-77380394 MsG0780040424.01.T01:CDS
TCTTGCTGTTGGGTACTACA+GGG 0.572141 7:-77380411 MsG0780040424.01.T01:CDS
TAACTTTCCGTTGAGTTGCA+GGG 0.573150 7:-77381906 MsG0780040424.01.T01:five_prime_UTR
CCAAGGGATCTGATCGCCGC+CGG 0.574729 7:+77381815 None:intergenic
TAGTTAACCGTCGTCGATGG+AGG 0.589229 7:-77381880 MsG0780040424.01.T01:exon
GAGCATAATGAGGTTGTCAT+GGG 0.590973 7:-77381366 MsG0780040424.01.T01:CDS
CTGTGAAAGAGAAGTTGCAG+AGG 0.603501 7:-77381423 MsG0780040424.01.T01:CDS
TGACATCACGGTCGCACCAA+GGG 0.605500 7:+77381799 None:intergenic
CCACTGATTATCCTAGGAGT+CGG 0.611803 7:-77380388 MsG0780040424.01.T01:CDS
CGCCGGTAAAGAAGTTTCCG+AGG 0.620017 7:+77381832 None:intergenic
GCCGGTAAAGAAGTTTCCGA+GGG 0.620980 7:+77381833 None:intergenic
ACTATCACCCTGCAACTCAA+CGG 0.626960 7:+77381899 None:intergenic
CTCGGAAACTTCTTTACCGG+CGG 0.628306 7:-77381831 MsG0780040424.01.T01:CDS
ATGACATCACGGTCGCACCA+AGG 0.644581 7:+77381798 None:intergenic
GTAAGAATGAGAGCATAATG+AGG 0.656100 7:-77381376 MsG0780040424.01.T01:CDS
TTTCGAGCATGGCAATCCCA+CGG 0.664812 7:+77381309 None:intergenic
AACAAGAAGATATTCACCGT+GGG 0.666532 7:-77381325 MsG0780040424.01.T01:CDS
AAGTTACAGAGATGACATCA+CGG 0.723970 7:+77381787 None:intergenic
CCGACTCCTAGGATAATCAG+TGG 0.729427 7:+77380388 None:intergenic
CAACAAGAAGATATTCACCG+TGG 0.736975 7:-77381326 MsG0780040424.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAATATAAGAAAAAATATT+CGG + Chr7:77381023-77381042 None:intergenic 10.0%
!! AATCATATTCATATGTTCAT+TGG - Chr7:77381117-77381136 MsG0780040424.01.T01:intron 20.0%
!! ATAATAATTATGGATGTACA+AGG + Chr7:77381801-77381820 None:intergenic 20.0%
!! ATGCAATACAACTAAAATAA+TGG - Chr7:77381385-77381404 MsG0780040424.01.T01:CDS 20.0%
!! TACAAAAAAACAAGAAATAC+AGG - Chr7:77380364-77380383 MsG0780040424.01.T01:CDS 20.0%
!! TCAAAAAAGCTATTAATTTC+CGG + Chr7:77380607-77380626 None:intergenic 20.0%
!! TTCTGATTATTATTCTGTAT+AGG - Chr7:77381039-77381058 MsG0780040424.01.T01:intron 20.0%
!!! ATTTTGATTTGTTTGAGTTT+AGG - Chr7:77380727-77380746 MsG0780040424.01.T01:intron 20.0%
!!! TTTTGATTTGTTTGAGTTTA+GGG - Chr7:77380728-77380747 MsG0780040424.01.T01:intron 20.0%
! AAAAAAACACATAGCAAGAA+AGG + Chr7:77381717-77381736 None:intergenic 25.0%
! AACATCAATCATATCTCTTT+AGG + Chr7:77381747-77381766 None:intergenic 25.0%
! AAGAAATAACATGAATTGAG+AGG + Chr7:77380550-77380569 None:intergenic 25.0%
! ACATCAATCATATCTCTTTA+GGG + Chr7:77381746-77381765 None:intergenic 25.0%
! ACTCAAACAAATCAAAATTG+AGG + Chr7:77380725-77380744 None:intergenic 25.0%
! AGAAATAACATGAATTGAGA+GGG + Chr7:77380549-77380568 None:intergenic 25.0%
! ATTATTCTGTATAGGTTCAT+TGG - Chr7:77381047-77381066 MsG0780040424.01.T01:intron 25.0%
! CAAATTAATGTAACTTCTCT+TGG - Chr7:77381272-77381291 MsG0780040424.01.T01:intron 25.0%
! CTCAAACAAATCAAAATTGA+GGG + Chr7:77380724-77380743 None:intergenic 25.0%
! TATCACACAAACTTTAATGT+AGG - Chr7:77381462-77381481 MsG0780040424.01.T01:CDS 25.0%
!! ATTTTCAATCTGGTTATGAA+AGG + Chr7:77380756-77380775 None:intergenic 25.0%
!!! ACCAGATTGAAAATTTTAAC+TGG - Chr7:77380762-77380781 MsG0780040424.01.T01:intron 25.0%
!!! CCAGATTGAAAATTTTAACT+GGG - Chr7:77380763-77380782 MsG0780040424.01.T01:intron 25.0%
!!! TTTCATTGCATTGTTTTGAA+GGG - Chr7:77380847-77380866 MsG0780040424.01.T01:intron 25.0%
!!! TTTTCATTGCATTGTTTTGA+AGG - Chr7:77380846-77380865 MsG0780040424.01.T01:intron 25.0%
ACAAACTTTAATGTAGGAGT+AGG - Chr7:77381468-77381487 MsG0780040424.01.T01:CDS 30.0%
AGTAATTATACTTTCGAGCA+TGG + Chr7:77380998-77381017 None:intergenic 30.0%
GCTGCGAATCATAATAATTA+TGG + Chr7:77381811-77381830 None:intergenic 30.0%
TATTATGCCCTTTATATGCA+TGG - Chr7:77381771-77381790 MsG0780040424.01.T01:intron 30.0%
TGAATTGCAAATACACTGTT+TGG - Chr7:77380810-77380829 MsG0780040424.01.T01:intron 30.0%
TTACTAAATTAGCGTGTGAA+GGG - Chr7:77381500-77381519 MsG0780040424.01.T01:intron 30.0%
TTTACTAAATTAGCGTGTGA+AGG - Chr7:77381499-77381518 MsG0780040424.01.T01:intron 30.0%
! CCCAGTTAAAATTTTCAATC+TGG + Chr7:77380766-77380785 None:intergenic 30.0%
! TACATTAATTTGAGCACAAC+AGG + Chr7:77381264-77381283 None:intergenic 30.0%
!!! ATTTTAACTGGGATTATTGC+TGG - Chr7:77380774-77380793 MsG0780040424.01.T01:intron 30.0%
AAAACACATAGCAAGAAAGG+AGG + Chr7:77381714-77381733 None:intergenic 35.0%
AAACACATAGCAAGAAAGGA+GGG + Chr7:77381713-77381732 None:intergenic 35.0%
AACAAGAAGATATTCACCGT+GGG - Chr7:77380968-77380987 MsG0780040424.01.T01:intron 35.0%
AAGTTACAGAGATGACATCA+CGG + Chr7:77380509-77380528 None:intergenic 35.0%
ACAACTAGCAATGAAGACAT+TGG + Chr7:77381310-77381329 None:intergenic 35.0%
ACTCAGAATTTCAATTGTGC+CGG - Chr7:77380585-77380604 MsG0780040424.01.T01:intron 35.0%
ACTTTAATGTAGGAGTAGGA+CGG - Chr7:77381472-77381491 MsG0780040424.01.T01:CDS 35.0%
AGCTTCTCTCTTTGATGTAA+AGG + Chr7:77381856-77381875 None:intergenic 35.0%
AGCTTGAGAGGAAAATCTAA+CGG - Chr7:77381541-77381560 MsG0780040424.01.T01:intron 35.0%
ATCACAAGACGTAGAATAGA+CGG + Chr7:77381148-77381167 None:intergenic 35.0%
ATTATGATTCGCAGCTTCTT+TGG - Chr7:77381816-77381835 MsG0780040424.01.T01:CDS 35.0%
GCAGTTCGAATTATTCATTC+AGG + Chr7:77381349-77381368 None:intergenic 35.0%
GTTGGAGAAAGTTTAGATCA+TGG + Chr7:77381577-77381596 None:intergenic 35.0%
TCAACGCTATATTGCATACT+CGG - Chr7:77381179-77381198 MsG0780040424.01.T01:intron 35.0%
TCAAGCTGGTCCAATTATAA+TGG + Chr7:77381528-77381547 None:intergenic 35.0%
TTATGATTCGCAGCTTCTTT+GGG - Chr7:77381817-77381836 MsG0780040424.01.T01:CDS 35.0%
TTCGAATTATTCATTCAGGC+TGG + Chr7:77381345-77381364 None:intergenic 35.0%
TTCTACGTCTTGTGATTTCT+TGG - Chr7:77381152-77381171 MsG0780040424.01.T01:intron 35.0%
! AAAATTGAGGGCGCAAAATA+TGG + Chr7:77380712-77380731 None:intergenic 35.0%
! AAGCTTTATCTTCTTGCTGT+TGG - Chr7:77381871-77381890 MsG0780040424.01.T01:exon 35.0%
! GTAAGAATGAGAGCATAATG+AGG - Chr7:77380917-77380936 MsG0780040424.01.T01:intron 35.0%
!! AGCTTTATCTTCTTGCTGTT+GGG - Chr7:77381872-77381891 MsG0780040424.01.T01:exon 35.0%
AACTGGGATTATTGCTGGAA+TGG - Chr7:77380779-77380798 MsG0780040424.01.T01:intron 40.0%
AAGGTCAGCCATGCATATAA+AGG + Chr7:77381782-77381801 None:intergenic 40.0%
AGAGCATAATGAGGTTGTCA+TGG - Chr7:77380926-77380945 MsG0780040424.01.T01:intron 40.0%
AGGTCAGCCATGCATATAAA+GGG + Chr7:77381781-77381800 None:intergenic 40.0%
CAACAAGAAGATATTCACCG+TGG - Chr7:77380967-77380986 MsG0780040424.01.T01:intron 40.0%
GAGCATAATGAGGTTGTCAT+GGG - Chr7:77380927-77380946 MsG0780040424.01.T01:intron 40.0%
TAGCAGCTATATGTGACAGA+AGG + Chr7:77381226-77381245 None:intergenic 40.0%
TATAATTGGACCAGCTTGAG+AGG - Chr7:77381529-77381548 MsG0780040424.01.T01:intron 40.0%
TCACAAGACGTAGAATAGAC+GGG + Chr7:77381147-77381166 None:intergenic 40.0%
! GTTAGATTTTCCTCTCAAGC+TGG + Chr7:77381542-77381561 None:intergenic 40.0%
! TAACTTTCCGTTGAGTTGCA+GGG - Chr7:77380387-77380406 MsG0780040424.01.T01:CDS 40.0%
!! GTGAAGGGCACCATTATAAT+TGG - Chr7:77381515-77381534 MsG0780040424.01.T01:intron 40.0%
ACTATCACCCTGCAACTCAA+CGG + Chr7:77380397-77380416 None:intergenic 45.0%
AGAAGTCTGCTTTGCATCGT+TGG + Chr7:77381595-77381614 None:intergenic 45.0%
AGAGGGATGAGAGAAAGAAG+AGG + Chr7:77380532-77380551 None:intergenic 45.0%
CATGGGCACTTGTTCATTCA+AGG - Chr7:77380944-77380963 MsG0780040424.01.T01:intron 45.0%
CTGTGAAAGAGAAGTTGCAG+AGG - Chr7:77380870-77380889 MsG0780040424.01.T01:intron 45.0%
GAAGTTGCAGAGGCTTCTAA+TGG - Chr7:77380880-77380899 MsG0780040424.01.T01:intron 45.0%
GGAAGCTTCTTTGAATCCCT+CGG - Chr7:77380444-77380463 MsG0780040424.01.T01:CDS 45.0%
TAGACAACTTCCTCGCCTTA+GGG - Chr7:77381677-77381696 MsG0780040424.01.T01:intron 45.0%
TCCCTCGGAAACTTCTTTAC+CGG - Chr7:77380459-77380478 MsG0780040424.01.T01:CDS 45.0%
TCGTCGATGGAGGCTAAAAT+CGG - Chr7:77380423-77380442 MsG0780040424.01.T01:CDS 45.0%
TGATAGTTAACCGTCGTCGA+TGG - Chr7:77380410-77380429 MsG0780040424.01.T01:CDS 45.0%
TTAATGTAGGAGTAGGACGG+TGG - Chr7:77381475-77381494 MsG0780040424.01.T01:CDS 45.0%
TTAGACAACTTCCTCGCCTT+AGG - Chr7:77381676-77381695 MsG0780040424.01.T01:intron 45.0%
! AGATCAGCCTGAGATGCTTT+TGG - Chr7:77381646-77381665 MsG0780040424.01.T01:intron 45.0%
! CCACTGATTATCCTAGGAGT+CGG - Chr7:77381905-77381924 MsG0780040424.01.T01:five_prime_UTR 45.0%
! GTAACTTTCCGTTGAGTTGC+AGG - Chr7:77380386-77380405 MsG0780040424.01.T01:CDS 45.0%
!! ATTCACGCCAAAAGCATCTC+AGG + Chr7:77381656-77381675 None:intergenic 45.0%
!! ATTTTAGCCTCCATCGACGA+CGG + Chr7:77380423-77380442 None:intergenic 45.0%
!! TCTTGCTGTTGGGTACTACA+GGG - Chr7:77381882-77381901 MsG0780040424.01.T01:exon 45.0%
!! TTCTTGCTGTTGGGTACTAC+AGG - Chr7:77381881-77381900 MsG0780040424.01.T01:exon 45.0%
CCGACTCCTAGGATAATCAG+TGG + Chr7:77381908-77381927 None:intergenic 50.0%
CTCGGAAACTTCTTTACCGG+CGG - Chr7:77380462-77380481 MsG0780040424.01.T01:CDS 50.0%
GCCGGTAAAGAAGTTTCCGA+GGG + Chr7:77380463-77380482 None:intergenic 50.0%
TAGTTAACCGTCGTCGATGG+AGG - Chr7:77380413-77380432 MsG0780040424.01.T01:CDS 50.0%
TTTCGAGCATGGCAATCCCA+CGG + Chr7:77380987-77381006 None:intergenic 50.0%
! ACAGGGCCACTGATTATCCT+AGG - Chr7:77381899-77381918 MsG0780040424.01.T01:five_prime_UTR 50.0%
!! GCAGCTTCTGGAGCAATGTT+TGG - Chr7:77381927-77381946 MsG0780040424.01.T01:five_prime_UTR 50.0%
!! GCTTCTGGAGCAATGTTTGG+AGG - Chr7:77381930-77381949 MsG0780040424.01.T01:five_prime_UTR 50.0%
ATGACATCACGGTCGCACCA+AGG + Chr7:77380498-77380517 None:intergenic 55.0%
CAAGAAAGGAGGGACTGCAG+TGG + Chr7:77381703-77381722 None:intergenic 55.0%
CAATGACCCCAGAGCCACTT+CGG + Chr7:77381426-77381445 None:intergenic 55.0%
CCTTCAAAACGCCACCGAAG+TGG - Chr7:77381409-77381428 MsG0780040424.01.T01:CDS 55.0%
CGCCGGTAAAGAAGTTTCCG+AGG + Chr7:77380464-77380483 None:intergenic 55.0%
TGACATCACGGTCGCACCAA+GGG + Chr7:77380497-77380516 None:intergenic 55.0%
! CCACTTCGGTGGCGTTTTGA+AGG + Chr7:77381412-77381431 None:intergenic 55.0%
AAACGCCACCGAAGTGGCTC+TGG - Chr7:77381415-77381434 MsG0780040424.01.T01:CDS 60.0%
AACGCCACCGAAGTGGCTCT+GGG - Chr7:77381416-77381435 MsG0780040424.01.T01:CDS 60.0%
TCCTAGGAGTCGGCAGCTTC+TGG - Chr7:77381915-77381934 MsG0780040424.01.T01:five_prime_UTR 60.0%
!! TCCAGAAGCTGCCGACTCCT+AGG + Chr7:77381919-77381938 None:intergenic 60.0%
ACTGCAGTGGCCCTAAGGCG+AGG + Chr7:77381690-77381709 None:intergenic 65.0%
CCAAGGGATCTGATCGCCGC+CGG + Chr7:77380481-77380500 None:intergenic 65.0%
GAGGGACTGCAGTGGCCCTA+AGG + Chr7:77381695-77381714 None:intergenic 65.0%
TGACCCCAGAGCCACTTCGG+TGG + Chr7:77381423-77381442 None:intergenic 65.0%
! ACGCCACCGAAGTGGCTCTG+GGG - Chr7:77381417-77381436 MsG0780040424.01.T01:CDS 65.0%
CCGGCGGCGATCAGATCCCT+TGG - Chr7:77380478-77380497 MsG0780040424.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
Chr7 gene 77380343 77381972 77380343 ID=MsG0780040424.01;Name=MsG0780040424.01
Chr7 mRNA 77380343 77381972 77380343 ID=MsG0780040424.01.T01;Parent=MsG0780040424.01;Name=MsG0780040424.01.T01;_AED=0.50;_eAED=0.55;_QI=86|0.5|0.33|1|0.5|0.33|3|0|145
Chr7 exon 77380343 77380485 77380343 ID=MsG0780040424.01.T01:exon:14882;Parent=MsG0780040424.01.T01
Chr7 exon 77381309 77381510 77381309 ID=MsG0780040424.01.T01:exon:14881;Parent=MsG0780040424.01.T01
Chr7 exon 77381794 77381972 77381794 ID=MsG0780040424.01.T01:exon:14880;Parent=MsG0780040424.01.T01
Chr7 five_prime_UTR 77381887 77381972 77381887 ID=MsG0780040424.01.T01:five_prime_utr;Parent=MsG0780040424.01.T01
Chr7 CDS 77381794 77381886 77381794 ID=MsG0780040424.01.T01:cds;Parent=MsG0780040424.01.T01
Chr7 CDS 77381309 77381510 77381309 ID=MsG0780040424.01.T01:cds;Parent=MsG0780040424.01.T01
Chr7 CDS 77380343 77380485 77380343 ID=MsG0780040424.01.T01:cds;Parent=MsG0780040424.01.T01
Gene Sequence

>MsG0780040424.01.T01

ATGGAGGCTAAAATCGGAAGCTTCTTTGAATCCCTCGGAAACTTCTTTACCGGCGGCGATCAGATCCCTTGGTGCGACCGTGATGTCATCTCTCTTTGTGAATTGCAAATACACTGTTTGGTAGTCTTACATATTTTTCATTGCATTGTTTTGAAGGGCTGTGAAAGAGAAGTTGCAGAGGCTTCTAATGGTGACTCTGATGAGCGTAAGAATGAGAGCATAATGAGGTTGTCATGGGCACTTGTTCATTCAAGGCAACAAGAAGATATTCACCGTGGGATTGCCATGCTCGAAACTTCTTTGGGCAATGATAGAAGTCCTTTACATCAAAGAGAGAAGCTTTATCTTCTTGCTGTTGGGTACTACAGGGCCACTGATTATCCTAGGAGTCGGCAGCTTCTGGAGCAATGTTTGGAGGTCTGTCTTTCTCACTTATAG

Protein sequence

>MsG0780040424.01.T01

MEAKIGSFFESLGNFFTGGDQIPWCDRDVISLCELQIHCLVVLHIFHCIVLKGCEREVAEASNGDSDERKNESIMRLSWALVHSRQQEDIHRGIAMLETSLGNDRSPLHQREKLYLLAVGYYRATDYPRSRQLLEQCLEVCLSHL*