Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045528.01.T01 | XP_003629109.2 | 94.828 | 116 | 6 | 0 | 1 | 116 | 4 | 119 | 1.72E-73 | 235 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045528.01.T01 | Q8GYH5 | 46.018 | 113 | 61 | 0 | 4 | 116 | 1 | 113 | 3.57E-29 | 111 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045528.01.T01 | A0A396GMV0 | 94.828 | 116 | 6 | 0 | 1 | 116 | 4 | 119 | 8.23e-74 | 235 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0880045528.01 | MsG0880046273.01 | 0.804167 | 2.459856e-49 | 1.259505e-46 |
| MsG0780040497.01 | MsG0880045528.01 | 0.822988 | 1.764083e-53 | 1.499816e-50 |
| MsG0680034418.01 | MsG0880045528.01 | 0.818959 | 1.492785e-52 | 1.133728e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045528.01.T01 | MTR_8g073260 | 94.828 | 116 | 6 | 0 | 1 | 116 | 4 | 119 | 2.31e-77 | 235 |
| MsG0880045528.01.T01 | MTR_3g016210 | 74.312 | 109 | 28 | 0 | 7 | 115 | 9 | 117 | 1.09e-56 | 181 |
| MsG0880045528.01.T01 | MTR_8g039620 | 70.248 | 121 | 30 | 1 | 1 | 115 | 1 | 121 | 1.52e-56 | 181 |
| MsG0880045528.01.T01 | MTR_1g059930 | 75.229 | 109 | 27 | 0 | 7 | 115 | 11 | 119 | 8.96e-56 | 179 |
| MsG0880045528.01.T01 | MTR_8g068990 | 73.636 | 110 | 29 | 0 | 7 | 116 | 11 | 120 | 5.17e-54 | 175 |
| MsG0880045528.01.T01 | MTR_8g072510 | 70.000 | 110 | 33 | 0 | 7 | 116 | 9 | 118 | 1.35e-52 | 171 |
| MsG0880045528.01.T01 | MTR_8g062410 | 70.000 | 110 | 33 | 0 | 7 | 116 | 9 | 118 | 1.10e-50 | 166 |
| MsG0880045528.01.T01 | MTR_7g021090 | 67.273 | 110 | 36 | 0 | 7 | 116 | 8 | 117 | 5.56e-50 | 163 |
| MsG0880045528.01.T01 | MTR_3g105650 | 66.957 | 115 | 35 | 1 | 1 | 115 | 1 | 112 | 4.32e-49 | 161 |
| MsG0880045528.01.T01 | MTR_7g451420 | 67.890 | 109 | 35 | 0 | 7 | 115 | 8 | 116 | 5.79e-49 | 158 |
| MsG0880045528.01.T01 | MTR_8g069880 | 62.069 | 116 | 43 | 1 | 1 | 116 | 1 | 115 | 6.24e-42 | 143 |
| MsG0880045528.01.T01 | MTR_8g467780 | 59.292 | 113 | 46 | 0 | 4 | 116 | 7 | 119 | 3.15e-41 | 141 |
| MsG0880045528.01.T01 | MTR_8g066670 | 64.545 | 110 | 39 | 0 | 6 | 115 | 1073 | 1182 | 4.83e-41 | 144 |
| MsG0880045528.01.T01 | MTR_8g073275 | 77.778 | 81 | 4 | 1 | 50 | 116 | 1 | 81 | 9.87e-36 | 127 |
| MsG0880045528.01.T01 | MTR_8g040930 | 74.627 | 67 | 17 | 0 | 50 | 116 | 1 | 67 | 2.94e-29 | 108 |
| MsG0880045528.01.T01 | MTR_8g041240 | 74.627 | 67 | 17 | 0 | 50 | 116 | 1 | 67 | 2.94e-29 | 108 |
| MsG0880045528.01.T01 | MTR_1g069110 | 34.286 | 105 | 69 | 0 | 11 | 115 | 21 | 125 | 4.24e-16 | 73.2 |
| MsG0880045528.01.T01 | MTR_7g099570 | 32.381 | 105 | 71 | 0 | 11 | 115 | 12 | 116 | 1.55e-15 | 71.6 |
| MsG0880045528.01.T01 | MTR_7g099580 | 29.524 | 105 | 74 | 0 | 11 | 115 | 15 | 119 | 1.13e-12 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045528.01.T01 | AT4G10720 | 51.818 | 110 | 53 | 0 | 4 | 113 | 1 | 110 | 1.05e-34 | 123 |
| MsG0880045528.01.T01 | AT4G10720 | 51.818 | 110 | 53 | 0 | 4 | 113 | 1 | 110 | 1.59e-34 | 123 |
| MsG0880045528.01.T01 | AT1G14480 | 49.550 | 111 | 56 | 0 | 4 | 114 | 1 | 111 | 1.48e-33 | 120 |
| MsG0880045528.01.T01 | AT1G14480 | 49.550 | 111 | 56 | 0 | 4 | 114 | 1 | 111 | 2.75e-33 | 120 |
| MsG0880045528.01.T01 | AT5G54620 | 48.649 | 111 | 57 | 0 | 4 | 114 | 1 | 111 | 4.66e-32 | 117 |
| MsG0880045528.01.T01 | AT5G15500 | 52.294 | 109 | 52 | 0 | 8 | 116 | 6 | 114 | 4.72e-32 | 117 |
| MsG0880045528.01.T01 | AT1G14500 | 46.847 | 111 | 59 | 0 | 4 | 114 | 1 | 111 | 1.34e-31 | 115 |
| MsG0880045528.01.T01 | AT5G54610 | 46.018 | 113 | 61 | 0 | 4 | 116 | 1 | 113 | 3.63e-30 | 111 |
Find 24 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCATAATTTCAGTGGCAAAT+TGG | 0.236402 | 8:+60597952 | None:intergenic |
| TGAGGAGGATCCATATGTTT+TGG | 0.280435 | 8:-60598039 | MsG0880045528.01.T01:CDS |
| AACGAAGCACCATACTCTTT+TGG | 0.338315 | 8:+60597856 | None:intergenic |
| TCGTTTCGTAGACATGAATA+AGG | 0.350127 | 8:-60597838 | MsG0880045528.01.T01:CDS |
| TCTATATATTCCAAAACATA+TGG | 0.396171 | 8:+60598029 | None:intergenic |
| GCAAAACAACCAAAAGAGTA+TGG | 0.398876 | 8:-60597865 | MsG0880045528.01.T01:CDS |
| GATTGAAATCTTCATTTGCT+TGG | 0.413951 | 8:-60597929 | MsG0880045528.01.T01:CDS |
| TGTAGAGGAGTTTCAACAAA+TGG | 0.445043 | 8:+60597999 | None:intergenic |
| GTGAAGGTTGCAACTCTAGA+AGG | 0.445366 | 8:-60598089 | MsG0880045528.01.T01:CDS |
| TCTACATATAGCTGCATCAA+TGG | 0.456263 | 8:-60597982 | MsG0880045528.01.T01:CDS |
| AAGGAACTTGTTAGAATCAA+AGG | 0.479455 | 8:-60597819 | MsG0880045528.01.T01:CDS |
| CTACATATAGCTGCATCAAT+GGG | 0.510700 | 8:-60597981 | MsG0880045528.01.T01:CDS |
| GTTAGAATCAAAGGAAGGGA+AGG | 0.542029 | 8:-60597810 | MsG0880045528.01.T01:CDS |
| CCTTCTCTATACACTAATTG+AGG | 0.550283 | 8:-60598057 | MsG0880045528.01.T01:CDS |
| TTTCAATCTCATAATTTCAG+TGG | 0.552567 | 8:+60597944 | None:intergenic |
| ACTTAACAAGCAAGATGAAG+AGG | 0.568635 | 8:+60597780 | None:intergenic |
| CCTCAATTAGTGTATAGAGA+AGG | 0.577645 | 8:+60598057 | None:intergenic |
| AACTTGTTAGAATCAAAGGA+AGG | 0.583415 | 8:-60597815 | MsG0880045528.01.T01:CDS |
| GCTTGGAAGCTAAATCAACA+AGG | 0.604414 | 8:-60597912 | MsG0880045528.01.T01:CDS |
| ACTTGTTAGAATCAAAGGAA+GGG | 0.615429 | 8:-60597814 | MsG0880045528.01.T01:CDS |
| TGATATTGATTACAAAGTGA+AGG | 0.624755 | 8:-60598105 | MsG0880045528.01.T01:CDS |
| CTTGGAAGCTAAATCAACAA+GGG | 0.626538 | 8:-60597911 | MsG0880045528.01.T01:CDS |
| ATTGATGCAGCTATATGTAG+AGG | 0.639713 | 8:+60597984 | None:intergenic |
| TCTCTATACACTAATTGAGG+AGG | 0.662445 | 8:-60598054 | MsG0880045528.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCTATATATTCCAAAACATA+TGG | + | Chr8:60597866-60597885 | None:intergenic | 20.0% |
| ! | TTTCAATCTCATAATTTCAG+TGG | + | Chr8:60597951-60597970 | None:intergenic | 25.0% |
| !! | TGATATTGATTACAAAGTGA+AGG | - | Chr8:60597787-60597806 | MsG0880045528.01.T01:CDS | 25.0% |
| GATTGAAATCTTCATTTGCT+TGG | - | Chr8:60597963-60597982 | MsG0880045528.01.T01:CDS | 30.0% | |
| TCATAATTTCAGTGGCAAAT+TGG | + | Chr8:60597943-60597962 | None:intergenic | 30.0% | |
| ! | ACTTGTTAGAATCAAAGGAA+GGG | - | Chr8:60598078-60598097 | MsG0880045528.01.T01:CDS | 30.0% |
| !! | AACTTGTTAGAATCAAAGGA+AGG | - | Chr8:60598077-60598096 | MsG0880045528.01.T01:CDS | 30.0% |
| !! | AAGGAACTTGTTAGAATCAA+AGG | - | Chr8:60598073-60598092 | MsG0880045528.01.T01:CDS | 30.0% |
| ATTGATGCAGCTATATGTAG+AGG | + | Chr8:60597911-60597930 | None:intergenic | 35.0% | |
| CCTCAATTAGTGTATAGAGA+AGG | + | Chr8:60597838-60597857 | None:intergenic | 35.0% | |
| CCTTCTCTATACACTAATTG+AGG | - | Chr8:60597835-60597854 | MsG0880045528.01.T01:CDS | 35.0% | |
| CTACATATAGCTGCATCAAT+GGG | - | Chr8:60597911-60597930 | MsG0880045528.01.T01:CDS | 35.0% | |
| CTTGGAAGCTAAATCAACAA+GGG | - | Chr8:60597981-60598000 | MsG0880045528.01.T01:CDS | 35.0% | |
| GCAAAACAACCAAAAGAGTA+TGG | - | Chr8:60598027-60598046 | MsG0880045528.01.T01:CDS | 35.0% | |
| TCGTTTCGTAGACATGAATA+AGG | - | Chr8:60598054-60598073 | MsG0880045528.01.T01:CDS | 35.0% | |
| TCTACATATAGCTGCATCAA+TGG | - | Chr8:60597910-60597929 | MsG0880045528.01.T01:CDS | 35.0% | |
| TCTCTATACACTAATTGAGG+AGG | - | Chr8:60597838-60597857 | MsG0880045528.01.T01:CDS | 35.0% | |
| TGTAGAGGAGTTTCAACAAA+TGG | + | Chr8:60597896-60597915 | None:intergenic | 35.0% | |
| !! | ACTCTTTTGGTTGTTTTGCA+TGG | + | Chr8:60598026-60598045 | None:intergenic | 35.0% |
| GCTTGGAAGCTAAATCAACA+AGG | - | Chr8:60597980-60597999 | MsG0880045528.01.T01:CDS | 40.0% | |
| GTTAGAATCAAAGGAAGGGA+AGG | - | Chr8:60598082-60598101 | MsG0880045528.01.T01:CDS | 40.0% | |
| ! | TGAGGAGGATCCATATGTTT+TGG | - | Chr8:60597853-60597872 | MsG0880045528.01.T01:CDS | 40.0% |
| !! | AACGAAGCACCATACTCTTT+TGG | + | Chr8:60598039-60598058 | None:intergenic | 40.0% |
| !! | TTTGGTTGTTTTGCATGGCA+AGG | + | Chr8:60598021-60598040 | None:intergenic | 40.0% |
| GTGAAGGTTGCAACTCTAGA+AGG | - | Chr8:60597803-60597822 | MsG0880045528.01.T01:CDS | 45.0% | |
| ! | TTTTGCATGGCAAGGTGGAT+TGG | + | Chr8:60598013-60598032 | None:intergenic | 45.0% |
| !! | GGTTGTTTTGCATGGCAAGG+TGG | + | Chr8:60598018-60598037 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 60597782 | 60598132 | 60597782 | ID=MsG0880045528.01;Name=MsG0880045528.01 |
| Chr8 | mRNA | 60597782 | 60598132 | 60597782 | ID=MsG0880045528.01.T01;Parent=MsG0880045528.01;Name=MsG0880045528.01.T01;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|116 |
| Chr8 | exon | 60597782 | 60598132 | 60597782 | ID=MsG0880045528.01.T01:exon:12498;Parent=MsG0880045528.01.T01 |
| Chr8 | CDS | 60597782 | 60598132 | 60597782 | ID=MsG0880045528.01.T01:cds;Parent=MsG0880045528.01.T01 |
| Gene Sequence |
| Protein sequence |
