Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045629.01.T01 | XP_003629301.1 | 95.203 | 271 | 11 | 1 | 1 | 269 | 1 | 271 | 3.97E-178 | 503 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045629.01.T01 | A0A1P8B554 | 56.538 | 260 | 94 | 5 | 1 | 251 | 6 | 255 | 2.44E-95 | 284 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045629.01.T01 | G7LC27 | 95.203 | 271 | 11 | 1 | 1 | 269 | 1 | 271 | 1.89e-178 | 503 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045629.01.T01 | MTR_8g075550 | 95.203 | 271 | 11 | 1 | 1 | 269 | 1 | 271 | 0.0 | 503 |
| MsG0880045629.01.T01 | MTR_5g022310 | 72.963 | 270 | 68 | 2 | 1 | 268 | 1 | 267 | 2.04e-140 | 397 |
| MsG0880045629.01.T01 | MTR_5g022310 | 73.152 | 257 | 64 | 2 | 1 | 255 | 1 | 254 | 5.64e-133 | 377 |
| MsG0880045629.01.T01 | MTR_8g037890 | 71.375 | 269 | 65 | 3 | 1 | 267 | 1 | 259 | 1.31e-132 | 378 |
| MsG0880045629.01.T01 | MTR_8g056820 | 69.466 | 262 | 71 | 2 | 1 | 260 | 1 | 255 | 9.35e-127 | 361 |
| MsG0880045629.01.T01 | MTR_1g025420 | 66.406 | 256 | 77 | 6 | 13 | 265 | 9 | 258 | 4.81e-115 | 334 |
| MsG0880045629.01.T01 | MTR_1g025420 | 68.033 | 244 | 70 | 5 | 13 | 253 | 9 | 247 | 2.64e-114 | 330 |
| MsG0880045629.01.T01 | MTR_3g114030 | 69.231 | 234 | 65 | 4 | 20 | 251 | 1 | 229 | 3.53e-111 | 321 |
| MsG0880045629.01.T01 | MTR_4g073730 | 63.052 | 249 | 82 | 4 | 9 | 251 | 3 | 247 | 4.25e-106 | 310 |
| MsG0880045629.01.T01 | MTR_4g073730 | 63.052 | 249 | 82 | 4 | 9 | 251 | 3 | 247 | 8.42e-106 | 310 |
| MsG0880045629.01.T01 | MTR_4g073720 | 60.417 | 240 | 84 | 3 | 12 | 251 | 11 | 239 | 1.92e-96 | 287 |
| MsG0880045629.01.T01 | MTR_5g022350 | 58.367 | 245 | 86 | 4 | 7 | 251 | 14 | 242 | 9.31e-90 | 269 |
| MsG0880045629.01.T01 | MTR_8g036215 | 53.086 | 243 | 105 | 4 | 9 | 251 | 4 | 237 | 8.10e-87 | 259 |
| MsG0880045629.01.T01 | MTR_8g075510 | 53.200 | 250 | 99 | 6 | 9 | 255 | 13 | 247 | 6.49e-84 | 252 |
| MsG0880045629.01.T01 | MTR_8g075510 | 53.659 | 246 | 96 | 6 | 9 | 251 | 13 | 243 | 1.92e-83 | 253 |
| MsG0880045629.01.T01 | MTR_2g068030 | 54.148 | 229 | 96 | 3 | 25 | 251 | 22 | 243 | 3.87e-83 | 250 |
| MsG0880045629.01.T01 | MTR_1g062590 | 50.800 | 250 | 112 | 5 | 3 | 251 | 2 | 241 | 1.55e-82 | 248 |
| MsG0880045629.01.T01 | MTR_7g102380 | 49.799 | 249 | 114 | 5 | 4 | 251 | 2 | 240 | 1.96e-82 | 248 |
| MsG0880045629.01.T01 | MTR_8g107140 | 53.778 | 225 | 96 | 3 | 32 | 256 | 33 | 249 | 2.13e-81 | 246 |
| MsG0880045629.01.T01 | MTR_2g068655 | 52.500 | 240 | 105 | 3 | 22 | 259 | 27 | 259 | 2.32e-81 | 248 |
| MsG0880045629.01.T01 | MTR_2g067980 | 50.951 | 263 | 114 | 6 | 7 | 261 | 1 | 256 | 5.27e-81 | 263 |
| MsG0880045629.01.T01 | MTR_1g062660 | 51.004 | 249 | 109 | 5 | 7 | 254 | 8 | 244 | 9.60e-81 | 244 |
| MsG0880045629.01.T01 | MTR_6g079580 | 50.612 | 245 | 111 | 4 | 9 | 251 | 3 | 239 | 6.46e-80 | 241 |
| MsG0880045629.01.T01 | MTR_1g062340 | 51.667 | 240 | 105 | 5 | 13 | 251 | 12 | 241 | 7.48e-79 | 239 |
| MsG0880045629.01.T01 | MTR_1g062630 | 51.383 | 253 | 112 | 5 | 3 | 254 | 2 | 244 | 7.59e-79 | 239 |
| MsG0880045629.01.T01 | MTR_2g069660 | 49.206 | 252 | 117 | 3 | 1 | 251 | 1 | 242 | 4.71e-78 | 237 |
| MsG0880045629.01.T01 | MTR_1g062350 | 50.602 | 249 | 112 | 5 | 4 | 251 | 3 | 241 | 5.86e-78 | 236 |
| MsG0880045629.01.T01 | MTR_1g021945 | 55.085 | 236 | 94 | 5 | 23 | 255 | 22 | 248 | 6.04e-78 | 237 |
| MsG0880045629.01.T01 | MTR_1g062370 | 50.201 | 249 | 113 | 5 | 4 | 251 | 3 | 241 | 6.90e-78 | 236 |
| MsG0880045629.01.T01 | MTR_3g111620 | 53.043 | 230 | 99 | 4 | 28 | 256 | 29 | 250 | 1.14e-75 | 231 |
| MsG0880045629.01.T01 | MTR_7g062610 | 45.868 | 242 | 122 | 3 | 11 | 251 | 8 | 241 | 2.72e-70 | 217 |
| MsG0880045629.01.T01 | MTR_5g059200 | 47.581 | 248 | 114 | 6 | 10 | 256 | 12 | 244 | 6.12e-70 | 218 |
| MsG0880045629.01.T01 | MTR_3g068015 | 46.957 | 230 | 112 | 4 | 24 | 251 | 26 | 247 | 1.91e-68 | 213 |
| MsG0880045629.01.T01 | MTR_6g009480 | 41.961 | 255 | 137 | 5 | 1 | 251 | 4 | 251 | 6.93e-63 | 199 |
| MsG0880045629.01.T01 | MTR_1g062690 | 44.578 | 249 | 103 | 6 | 4 | 251 | 3 | 217 | 1.20e-61 | 194 |
| MsG0880045629.01.T01 | MTR_4g063630 | 44.309 | 246 | 123 | 8 | 10 | 251 | 16 | 251 | 3.08e-61 | 194 |
| MsG0880045629.01.T01 | MTR_5g023850 | 42.802 | 257 | 99 | 6 | 1 | 251 | 1 | 215 | 4.85e-61 | 193 |
| MsG0880045629.01.T01 | MTR_2g067990 | 48.133 | 241 | 92 | 9 | 22 | 261 | 23 | 231 | 5.33e-59 | 202 |
| MsG0880045629.01.T01 | MTR_5g010640 | 40.541 | 259 | 122 | 8 | 1 | 251 | 1 | 235 | 6.88e-49 | 162 |
| MsG0880045629.01.T01 | MTR_2g067970 | 48.404 | 188 | 83 | 6 | 69 | 251 | 3 | 181 | 1.06e-47 | 170 |
| MsG0880045629.01.T01 | MTR_5g022350 | 56.000 | 150 | 60 | 3 | 7 | 156 | 79 | 222 | 2.31e-45 | 152 |
| MsG0880045629.01.T01 | MTR_8g096900 | 38.189 | 254 | 125 | 9 | 1 | 251 | 23 | 247 | 4.91e-45 | 152 |
| MsG0880045629.01.T01 | MTR_5g010635 | 40.435 | 230 | 106 | 8 | 25 | 251 | 23 | 224 | 1.20e-44 | 151 |
| MsG0880045629.01.T01 | MTR_8g089020 | 50.365 | 137 | 58 | 2 | 111 | 247 | 33 | 159 | 1.67e-39 | 135 |
| MsG0880045629.01.T01 | MTR_2g063150 | 34.101 | 217 | 131 | 6 | 26 | 241 | 34 | 239 | 1.85e-37 | 133 |
| MsG0880045629.01.T01 | MTR_7g076360 | 36.986 | 219 | 69 | 3 | 24 | 242 | 6 | 155 | 2.27e-37 | 130 |
| MsG0880045629.01.T01 | MTR_1g062390 | 32.189 | 233 | 134 | 7 | 25 | 251 | 23 | 237 | 9.30e-31 | 115 |
| MsG0880045629.01.T01 | MTR_8g096910 | 44.444 | 144 | 70 | 4 | 9 | 151 | 5 | 139 | 2.90e-30 | 112 |
| MsG0880045629.01.T01 | MTR_8g096920 | 45.833 | 120 | 55 | 4 | 25 | 143 | 18 | 128 | 3.30e-23 | 93.6 |
| MsG0880045629.01.T01 | MTR_3g081550 | 46.465 | 99 | 47 | 3 | 111 | 208 | 16 | 109 | 7.06e-20 | 83.2 |
| MsG0880045629.01.T01 | MTR_1g062640 | 45.455 | 88 | 47 | 1 | 79 | 166 | 60 | 146 | 3.44e-18 | 79.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045629.01.T01 | AT4G36010 | 68.826 | 247 | 63 | 5 | 22 | 261 | 19 | 258 | 1.10e-114 | 332 |
| MsG0880045629.01.T01 | AT4G36010 | 68.826 | 247 | 63 | 5 | 22 | 261 | 19 | 258 | 1.10e-114 | 332 |
| MsG0880045629.01.T01 | AT2G17860 | 64.400 | 250 | 79 | 4 | 11 | 256 | 10 | 253 | 1.43e-112 | 325 |
| MsG0880045629.01.T01 | AT1G75800 | 62.692 | 260 | 90 | 6 | 11 | 267 | 7 | 262 | 1.92e-110 | 322 |
| MsG0880045629.01.T01 | AT1G20030 | 65.086 | 232 | 76 | 4 | 22 | 251 | 16 | 244 | 3.88e-106 | 311 |
| MsG0880045629.01.T01 | AT1G20030 | 65.502 | 229 | 74 | 4 | 25 | 251 | 2 | 227 | 2.43e-105 | 308 |
| MsG0880045629.01.T01 | AT4G38670 | 63.306 | 248 | 81 | 5 | 9 | 251 | 4 | 246 | 1.47e-104 | 306 |
| MsG0880045629.01.T01 | AT4G38670 | 63.306 | 248 | 81 | 5 | 9 | 251 | 4 | 246 | 1.47e-104 | 306 |
| MsG0880045629.01.T01 | AT4G38670 | 65.789 | 228 | 70 | 4 | 27 | 251 | 8 | 230 | 4.06e-101 | 296 |
| MsG0880045629.01.T01 | AT4G24180 | 56.538 | 260 | 94 | 5 | 1 | 251 | 6 | 255 | 2.49e-96 | 284 |
| MsG0880045629.01.T01 | AT4G24180 | 56.538 | 260 | 94 | 5 | 1 | 251 | 6 | 255 | 4.34e-96 | 285 |
| MsG0880045629.01.T01 | AT4G24180 | 56.589 | 258 | 92 | 5 | 1 | 251 | 6 | 250 | 3.26e-95 | 281 |
| MsG0880045629.01.T01 | AT4G24180 | 56.589 | 258 | 92 | 5 | 1 | 251 | 6 | 250 | 4.15e-95 | 283 |
| MsG0880045629.01.T01 | AT4G38660 | 58.678 | 242 | 89 | 3 | 10 | 251 | 14 | 244 | 2.38e-92 | 277 |
| MsG0880045629.01.T01 | AT4G38660 | 60.345 | 232 | 81 | 3 | 20 | 251 | 2 | 222 | 4.24e-92 | 275 |
| MsG0880045629.01.T01 | AT4G24180 | 55.285 | 246 | 91 | 5 | 1 | 237 | 6 | 241 | 5.84e-87 | 263 |
| MsG0880045629.01.T01 | AT1G75030 | 53.659 | 246 | 105 | 5 | 11 | 255 | 9 | 246 | 2.37e-86 | 258 |
| MsG0880045629.01.T01 | AT5G38280 | 52.209 | 249 | 111 | 4 | 7 | 251 | 5 | 249 | 3.14e-86 | 271 |
| MsG0880045629.01.T01 | AT5G38280 | 52.209 | 249 | 111 | 4 | 7 | 251 | 5 | 249 | 5.72e-86 | 270 |
| MsG0880045629.01.T01 | AT4G24180 | 55.328 | 244 | 89 | 5 | 1 | 237 | 6 | 236 | 8.16e-86 | 259 |
| MsG0880045629.01.T01 | AT5G24620 | 56.118 | 237 | 94 | 5 | 25 | 256 | 24 | 255 | 9.42e-85 | 260 |
| MsG0880045629.01.T01 | AT5G24620 | 56.118 | 237 | 94 | 5 | 25 | 256 | 79 | 310 | 2.72e-84 | 260 |
| MsG0880045629.01.T01 | AT5G24620 | 56.118 | 237 | 94 | 5 | 25 | 256 | 100 | 331 | 4.05e-84 | 261 |
| MsG0880045629.01.T01 | AT1G75050 | 53.252 | 246 | 106 | 5 | 11 | 255 | 9 | 246 | 8.60e-83 | 249 |
| MsG0880045629.01.T01 | AT1G75040 | 53.112 | 241 | 102 | 4 | 11 | 251 | 9 | 238 | 2.24e-81 | 245 |
| MsG0880045629.01.T01 | AT1G19320 | 52.610 | 249 | 101 | 6 | 11 | 252 | 9 | 247 | 4.84e-81 | 244 |
| MsG0880045629.01.T01 | AT1G18250 | 52.381 | 231 | 102 | 3 | 24 | 254 | 19 | 241 | 1.09e-80 | 244 |
| MsG0880045629.01.T01 | AT1G18250 | 52.609 | 230 | 101 | 3 | 25 | 254 | 21 | 242 | 1.13e-80 | 243 |
| MsG0880045629.01.T01 | AT1G73620 | 51.931 | 233 | 104 | 3 | 24 | 256 | 40 | 264 | 7.82e-80 | 242 |
| MsG0880045629.01.T01 | AT1G77700 | 49.393 | 247 | 109 | 6 | 11 | 256 | 45 | 276 | 5.56e-75 | 231 |
| MsG0880045629.01.T01 | AT1G77700 | 49.388 | 245 | 108 | 6 | 11 | 254 | 45 | 274 | 4.09e-74 | 230 |
| MsG0880045629.01.T01 | AT1G77700 | 50.439 | 228 | 98 | 5 | 27 | 254 | 90 | 302 | 6.72e-73 | 228 |
| MsG0880045629.01.T01 | AT5G02140 | 46.058 | 241 | 122 | 2 | 12 | 251 | 7 | 240 | 2.67e-68 | 212 |
| MsG0880045629.01.T01 | AT5G02140 | 45.714 | 245 | 125 | 2 | 12 | 255 | 7 | 244 | 3.22e-68 | 214 |
| MsG0880045629.01.T01 | AT4G36000 | 62.941 | 170 | 48 | 3 | 102 | 271 | 1 | 155 | 8.30e-65 | 202 |
| MsG0880045629.01.T01 | AT4G36000 | 62.941 | 170 | 48 | 3 | 102 | 271 | 1 | 155 | 8.30e-65 | 202 |
| MsG0880045629.01.T01 | AT2G28790 | 45.528 | 246 | 122 | 6 | 10 | 251 | 11 | 248 | 1.97e-63 | 200 |
| MsG0880045629.01.T01 | AT5G40020 | 45.259 | 232 | 119 | 4 | 24 | 254 | 26 | 250 | 2.10e-63 | 200 |
| MsG0880045629.01.T01 | AT2G28790 | 45.528 | 246 | 122 | 6 | 10 | 251 | 36 | 273 | 2.53e-63 | 200 |
| MsG0880045629.01.T01 | AT4G18253 | 43.320 | 247 | 124 | 4 | 11 | 254 | 7 | 240 | 4.22e-63 | 199 |
| MsG0880045629.01.T01 | AT1G70250 | 43.220 | 236 | 120 | 7 | 25 | 255 | 144 | 370 | 3.36e-57 | 196 |
| MsG0880045629.01.T01 | AT4G18250 | 43.443 | 244 | 125 | 5 | 25 | 267 | 56 | 287 | 5.30e-57 | 194 |
| MsG0880045629.01.T01 | AT4G11650 | 39.847 | 261 | 126 | 9 | 11 | 268 | 5 | 237 | 3.39e-50 | 166 |
| MsG0880045629.01.T01 | AT5G24620 | 59.375 | 128 | 44 | 3 | 133 | 256 | 15 | 138 | 7.90e-44 | 151 |
| MsG0880045629.01.T01 | AT2G24810 | 63.235 | 68 | 25 | 0 | 185 | 252 | 126 | 193 | 2.08e-25 | 100 |
Find 85 sgRNAs with CRISPR-Local
Find 115 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATCTTGGTCCGGCCGCATA+TGG | 0.247616 | 8:-61965046 | MsG0880045629.01.T01:CDS |
| GCCACTTGGTTCCACTGTTA+TGG | 0.270667 | 8:+61964836 | None:intergenic |
| CTTTGGATTCGCCCGGTTGT+AGG | 0.283686 | 8:+61965084 | None:intergenic |
| TCCATTTGGGAGTGGACATT+TGG | 0.291049 | 8:-61964354 | MsG0880045629.01.T01:CDS |
| CCACTTGGTTCCACTGTTAT+GGG | 0.324167 | 8:+61964837 | None:intergenic |
| ATCTTGGTCCGGCCGCATAT+GGG | 0.324930 | 8:-61965045 | MsG0880045629.01.T01:CDS |
| GGGAATGGAAAATTTCCATT+TGG | 0.351808 | 8:-61964368 | MsG0880045629.01.T01:CDS |
| AACAGGCTTCGCCCTACAAC+CGG | 0.360779 | 8:-61965096 | MsG0880045629.01.T01:CDS |
| CCGGCACCTGACAAAATTCC+CGG | 0.375719 | 8:+61965137 | None:intergenic |
| CCATTTGGGAGTGGACATTT+GGG | 0.386139 | 8:-61964353 | MsG0880045629.01.T01:CDS |
| AATGAGAAAACCACCTCTTA+AGG | 0.388901 | 8:+61965708 | None:intergenic |
| TTCACATTAAACGGTGCTAA+TGG | 0.389613 | 8:-61964903 | MsG0880045629.01.T01:CDS |
| GGAATGGAAAATTTCCATTT+GGG | 0.393942 | 8:-61964367 | MsG0880045629.01.T01:CDS |
| CTGCACGACAACCGGCTGTC+TGG | 0.400600 | 8:-61964802 | MsG0880045629.01.T01:CDS |
| GGTGGAAACTGCACGACAAC+CGG | 0.402032 | 8:-61964810 | MsG0880045629.01.T01:CDS |
| CTCACTACTCTCTTCACCTT+CGG | 0.406791 | 8:+61964731 | None:intergenic |
| GACAAAATTCCCGGCCAAAC+AGG | 0.419565 | 8:+61965146 | None:intergenic |
| TGTTGCTAGCTGTGCAGTTC+CGG | 0.421312 | 8:+61965118 | None:intergenic |
| GTGAACTCCGCGAGCGTCGC+TGG | 0.427013 | 8:+61964921 | None:intergenic |
| CAGTTTCCACCGCCGCCACT+TGG | 0.429907 | 8:+61964822 | None:intergenic |
| TGTGGGCATGGTGACAGCTT+TGG | 0.439164 | 8:+61965067 | None:intergenic |
| CTCGCGGAGTTCACATTAAA+CGG | 0.440614 | 8:-61964912 | MsG0880045629.01.T01:CDS |
| TATAGCTATGCTTATGATGA+TGG | 0.446692 | 8:-61964573 | MsG0880045629.01.T01:CDS |
| TTTGGATTCGCCCGGTTGTA+GGG | 0.455624 | 8:+61965085 | None:intergenic |
| GTGCAGCTATCCTGTTTGGC+CGG | 0.459261 | 8:-61965156 | MsG0880045629.01.T01:CDS |
| GCCAGTACATTCTACTTTGG+AGG | 0.461449 | 8:+61964959 | None:intergenic |
| ACAAGTGCAGCTATCCTGTT+TGG | 0.467259 | 8:-61965160 | MsG0880045629.01.T01:CDS |
| CCCAAATGTCCACTCCCAAA+TGG | 0.475216 | 8:+61964353 | None:intergenic |
| CGCGAGCGTCGCTGGAGGGA+TGG | 0.476877 | 8:+61964929 | None:intergenic |
| GGAAAATTTCCATTTGGGAG+TGG | 0.477173 | 8:-61964362 | MsG0880045629.01.T01:CDS |
| GTGGAACCAAGTGGCGGCGG+TGG | 0.479318 | 8:-61964828 | MsG0880045629.01.T01:CDS |
| GCATGCAGTTCGATAAAGTC+GGG | 0.484586 | 8:-61964389 | MsG0880045629.01.T01:intron |
| ACTGCACAGCTAGCAACAAC+AGG | 0.485951 | 8:-61965113 | MsG0880045629.01.T01:CDS |
| ACCATGCCCACATCTTGGTC+CGG | 0.489562 | 8:-61965056 | MsG0880045629.01.T01:CDS |
| GGCTGTCTGGTGGACTTAAA+CGG | 0.490034 | 8:-61964789 | MsG0880045629.01.T01:CDS |
| GGAAAATTCTCATGCATCAC+AGG | 0.490339 | 8:-61964993 | MsG0880045629.01.T01:CDS |
| AAGAACTGAGAGTATGAACT+TGG | 0.495630 | 8:+61964618 | None:intergenic |
| CTGTCACCATGCCCACATCT+TGG | 0.503432 | 8:-61965061 | MsG0880045629.01.T01:CDS |
| CACGACAACCGGCTGTCTGG+TGG | 0.507371 | 8:-61964799 | MsG0880045629.01.T01:CDS |
| GTGACAGCTTTGGATTCGCC+CGG | 0.522255 | 8:+61965077 | None:intergenic |
| TGCAGCTATCCTGTTTGGCC+GGG | 0.525531 | 8:-61965155 | MsG0880045629.01.T01:CDS |
| TTCTGTTCTACACATTCCAA+AGG | 0.529601 | 8:-61964507 | MsG0880045629.01.T01:CDS |
| TTGGGAGTGGACATTTGGGC+AGG | 0.531797 | 8:-61964349 | MsG0880045629.01.T01:CDS |
| GCTGCCAGTACATTCTACTT+TGG | 0.532355 | 8:+61964956 | None:intergenic |
| TTGGTTCCACTGTTATGGGA+AGG | 0.537141 | 8:+61964841 | None:intergenic |
| TCCTCCAAAGTAGAATGTAC+TGG | 0.546991 | 8:-61964960 | MsG0880045629.01.T01:CDS |
| AACTCCGCGAGCGTCGCTGG+AGG | 0.547782 | 8:+61964924 | None:intergenic |
| TCTGTTCTACACATTCCAAA+GGG | 0.547965 | 8:-61964506 | MsG0880045629.01.T01:intron |
| CTTGTTTACAATTGTAAATG+TGG | 0.550017 | 8:+61965178 | None:intergenic |
| GTTTAAGTCCACCAGACAGC+CGG | 0.553589 | 8:+61964791 | None:intergenic |
| ACTCCGCGAGCGTCGCTGGA+GGG | 0.555976 | 8:+61964925 | None:intergenic |
| CTATGCTCAACTGACCCCTC+CGG | 0.556358 | 8:-61965014 | MsG0880045629.01.T01:CDS |
| TCTTGGTCCGGCCGCATATG+GGG | 0.556818 | 8:-61965044 | MsG0880045629.01.T01:CDS |
| AAAAGCGCGTGTGAAGCATT+TGG | 0.559457 | 8:-61964693 | MsG0880045629.01.T01:CDS |
| GAAGGTTGTAACCGTCGACT+AGG | 0.560234 | 8:+61964859 | None:intergenic |
| GGTCAGTTGAGCATAGTGTT+CGG | 0.561722 | 8:+61965021 | None:intergenic |
| TATGCGGCCGGACCAAGATG+TGG | 0.564091 | 8:+61965049 | None:intergenic |
| AAAGTAGAATGTACTGGCAG+CGG | 0.569565 | 8:-61964954 | MsG0880045629.01.T01:CDS |
| AGAGGAACAAGTGAAGGTGC+TGG | 0.572579 | 8:+61964548 | None:intergenic |
| GATCCATTGTATTGTTGCAA+TGG | 0.576223 | 8:-61964669 | MsG0880045629.01.T01:CDS |
| TGCATGCAGTTCGATAAAGT+CGG | 0.579755 | 8:-61964390 | MsG0880045629.01.T01:intron |
| ATCTGCAGAGGAACAAGTGA+AGG | 0.582748 | 8:+61964542 | None:intergenic |
| CGCGTGTCCAACAGAACTGA+AGG | 0.585787 | 8:-61964766 | MsG0880045629.01.T01:CDS |
| ATAGTGTTCGGCCCCATATG+CGG | 0.585870 | 8:+61965033 | None:intergenic |
| AGTTCGATAAAGTCGGGGAA+TGG | 0.591689 | 8:-61964383 | MsG0880045629.01.T01:CDS |
| CATCCCTCCAGCGACGCTCG+CGG | 0.593566 | 8:-61964928 | MsG0880045629.01.T01:CDS |
| TGTTCGGCCCCATATGCGGC+CGG | 0.604068 | 8:+61965037 | None:intergenic |
| ATGCGGCCGGACCAAGATGT+GGG | 0.607602 | 8:+61965050 | None:intergenic |
| GCCGGACCAAGATGTGGGCA+TGG | 0.613267 | 8:+61965055 | None:intergenic |
| ACAGTGGAACCAAGTGGCGG+CGG | 0.614239 | 8:-61964831 | MsG0880045629.01.T01:CDS |
| ACAGGCTTCGCCCTACAACC+GGG | 0.616434 | 8:-61965095 | MsG0880045629.01.T01:CDS |
| TACGACGTCAGCCTAGTCGA+CGG | 0.620565 | 8:-61964870 | MsG0880045629.01.T01:CDS |
| GCGCCATTGCAACAATACAA+TGG | 0.633524 | 8:+61964666 | None:intergenic |
| AAGGTGAAAATTGCGGCCGA+AGG | 0.637905 | 8:-61964747 | MsG0880045629.01.T01:CDS |
| AGAACTGAAGGTGAAAATTG+CGG | 0.646741 | 8:-61964754 | MsG0880045629.01.T01:CDS |
| AGTAACGAGGTAATCTGCAG+AGG | 0.648578 | 8:+61964530 | None:intergenic |
| GAACTTGGCTTACAAGTCTG+CGG | 0.654802 | 8:+61964633 | None:intergenic |
| TCATGCATCACAGGAGACTG+TGG | 0.659195 | 8:-61964984 | MsG0880045629.01.T01:CDS |
| ATAACAGTGGAACCAAGTGG+CGG | 0.675042 | 8:-61964834 | MsG0880045629.01.T01:CDS |
| TAAGCATAGCTATAAGCGCG+TGG | 0.678782 | 8:+61964582 | None:intergenic |
| AGAGAGTAGTGAGAAGAGCG+TGG | 0.689722 | 8:-61964721 | MsG0880045629.01.T01:CDS |
| CATGCAGTTCGATAAAGTCG+GGG | 0.690951 | 8:-61964388 | MsG0880045629.01.T01:intron |
| TTACAACCTTCCCATAACAG+TGG | 0.699533 | 8:-61964847 | MsG0880045629.01.T01:CDS |
| CCCATAACAGTGGAACCAAG+TGG | 0.699801 | 8:-61964837 | MsG0880045629.01.T01:CDS |
| GTGTAGAACAGAAAGTAACG+AGG | 0.769697 | 8:+61964517 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAAATTTTTGAATTTGTTAT+AGG | + | Chr8:61964485-61964504 | None:intergenic | 10.0% |
| !! | CTACATTATTTACAAATAAT+TGG | + | Chr8:61964816-61964835 | None:intergenic | 15.0% |
| !! | ACAAATATCAAATATTCTAG+CGG | - | Chr8:61964790-61964809 | MsG0880045629.01.T01:CDS | 20.0% |
| !! | TGTTCATAGTAAATATTAAG+AGG | - | Chr8:61964671-61964690 | MsG0880045629.01.T01:CDS | 20.0% |
| !!! | ATGTTTTTTTCTTCATAACA+TGG | + | Chr8:61964562-61964581 | None:intergenic | 20.0% |
| !!! | CTCTAAGAATTAATGTTAAT+TGG | - | Chr8:61964845-61964864 | MsG0880045629.01.T01:CDS | 20.0% |
| !!! | TGTTTTTTTCTTCATAACAT+GGG | + | Chr8:61964561-61964580 | None:intergenic | 20.0% |
| ! | AATTAAACTACATTACCCTT+TGG | + | Chr8:61965612-61965631 | None:intergenic | 25.0% |
| ! | CTTGTTTACAATTGTAAATG+TGG | + | Chr8:61964925-61964944 | None:intergenic | 25.0% |
| !! | TTTTTCTTTCATTCCTTAAG+AGG | - | Chr8:61964379-61964398 | MsG0880045629.01.T01:CDS | 25.0% |
| !!! | GTTTTTTTCTTCATAACATG+GGG | + | Chr8:61964560-61964579 | None:intergenic | 25.0% |
| !!! | TTGAATTTGTTATAGGACTA+GGG | + | Chr8:61964478-61964497 | None:intergenic | 25.0% |
| !!! | TTTGAATTTGTTATAGGACT+AGG | + | Chr8:61964479-61964498 | None:intergenic | 25.0% |
| GAAGAATTATCATTTCGAAC+AGG | + | Chr8:61964721-61964740 | None:intergenic | 30.0% | |
| GGAATGGAAAATTTCCATTT+GGG | - | Chr8:61965733-61965752 | MsG0880045629.01.T01:CDS | 30.0% | |
| TAAGCTTGTGAAAATACCAT+AGG | - | Chr8:61964503-61964522 | MsG0880045629.01.T01:intron | 30.0% | |
| TATAGCTATGCTTATGATGA+TGG | - | Chr8:61965527-61965546 | MsG0880045629.01.T01:intron | 30.0% | |
| TTCATAACATGGGGTAATTA+GGG | + | Chr8:61964551-61964570 | None:intergenic | 30.0% | |
| TTGTGTAAACATTGCATACT+TGG | - | Chr8:61964874-61964893 | MsG0880045629.01.T01:CDS | 30.0% | |
| AATGAGAAAACCACCTCTTA+AGG | + | Chr8:61964395-61964414 | None:intergenic | 35.0% | |
| AGAACTGAAGGTGAAAATTG+CGG | - | Chr8:61965346-61965365 | MsG0880045629.01.T01:intron | 35.0% | |
| CTTCATAACATGGGGTAATT+AGG | + | Chr8:61964552-61964571 | None:intergenic | 35.0% | |
| GATCCATTGTATTGTTGCAA+TGG | - | Chr8:61965431-61965450 | MsG0880045629.01.T01:intron | 35.0% | |
| GGGAATGGAAAATTTCCATT+TGG | - | Chr8:61965732-61965751 | MsG0880045629.01.T01:CDS | 35.0% | |
| GTCTCAACATATCAAATGCT+TGG | + | Chr8:61964699-61964718 | None:intergenic | 35.0% | |
| GTCTCTCAATAAATGTCCTA+TGG | + | Chr8:61964522-61964541 | None:intergenic | 35.0% | |
| TCTGTTCTACACATTCCAAA+GGG | - | Chr8:61965594-61965613 | MsG0880045629.01.T01:intron | 35.0% | |
| TGCATGAGAAATAGAACAAG+TGG | + | Chr8:61964443-61964462 | None:intergenic | 35.0% | |
| TTCTGTTCTACACATTCCAA+AGG | - | Chr8:61965593-61965612 | MsG0880045629.01.T01:intron | 35.0% | |
| TTCTTTCATTCCTTAAGAGG+TGG | - | Chr8:61964382-61964401 | MsG0880045629.01.T01:CDS | 35.0% | |
| ! | AAGAACTGAGAGTATGAACT+TGG | + | Chr8:61965485-61965504 | None:intergenic | 35.0% |
| ! | ATTTTCACCTTCAGTTCTGT+TGG | + | Chr8:61965344-61965363 | None:intergenic | 35.0% |
| ! | TGTGATGCATGAGAATTTTC+CGG | + | Chr8:61965108-61965127 | None:intergenic | 35.0% |
| ! | TTCACATTAAACGGTGCTAA+TGG | - | Chr8:61965197-61965216 | MsG0880045629.01.T01:intron | 35.0% |
| GGAAAATTCTCATGCATCAC+AGG | - | Chr8:61965107-61965126 | MsG0880045629.01.T01:CDS | 40.0% | |
| GGAAAATTTCCATTTGGGAG+TGG | - | Chr8:61965738-61965757 | MsG0880045629.01.T01:CDS | 40.0% | |
| GTGTAGAACAGAAAGTAACG+AGG | + | Chr8:61965586-61965605 | None:intergenic | 40.0% | |
| TCCTCCAAAGTAGAATGTAC+TGG | - | Chr8:61965140-61965159 | MsG0880045629.01.T01:CDS | 40.0% | |
| TGCATGCAGTTCGATAAAGT+CGG | - | Chr8:61965710-61965729 | MsG0880045629.01.T01:intron | 40.0% | |
| TTACAACCTTCCCATAACAG+TGG | - | Chr8:61965253-61965272 | MsG0880045629.01.T01:intron | 40.0% | |
| ! | AAAGTAGAATGTACTGGCAG+CGG | - | Chr8:61965146-61965165 | MsG0880045629.01.T01:CDS | 40.0% |
| ! | ATGCATGAGAATTTTCCGGA+GGG | + | Chr8:61965104-61965123 | None:intergenic | 40.0% |
| !! | CATACTTGGTGTATCTATGC+AGG | - | Chr8:61964888-61964907 | MsG0880045629.01.T01:CDS | 40.0% |
| ACAAGTGCAGCTATCCTGTT+TGG | - | Chr8:61964940-61964959 | MsG0880045629.01.T01:CDS | 45.0% | |
| AGTAACGAGGTAATCTGCAG+AGG | + | Chr8:61965573-61965592 | None:intergenic | 45.0% | |
| AGTTCGATAAAGTCGGGGAA+TGG | - | Chr8:61965717-61965736 | MsG0880045629.01.T01:intron | 45.0% | |
| ATAACAGTGGAACCAAGTGG+CGG | - | Chr8:61965266-61965285 | MsG0880045629.01.T01:intron | 45.0% | |
| ATCTGCAGAGGAACAAGTGA+AGG | + | Chr8:61965561-61965580 | None:intergenic | 45.0% | |
| CATGCAGTTCGATAAAGTCG+GGG | - | Chr8:61965712-61965731 | MsG0880045629.01.T01:intron | 45.0% | |
| CATGGGGTAATTAGGGACTT+TGG | + | Chr8:61964544-61964563 | None:intergenic | 45.0% | |
| CCACTTGGTTCCACTGTTAT+GGG | + | Chr8:61965266-61965285 | None:intergenic | 45.0% | |
| CCATTTGGGAGTGGACATTT+GGG | - | Chr8:61965747-61965766 | MsG0880045629.01.T01:CDS | 45.0% | |
| CTCACTACTCTCTTCACCTT+CGG | + | Chr8:61965372-61965391 | None:intergenic | 45.0% | |
| CTCGCGGAGTTCACATTAAA+CGG | - | Chr8:61965188-61965207 | MsG0880045629.01.T01:CDS | 45.0% | |
| GAACTTGGCTTACAAGTCTG+CGG | + | Chr8:61965470-61965489 | None:intergenic | 45.0% | |
| GCATGCAGTTCGATAAAGTC+GGG | - | Chr8:61965711-61965730 | MsG0880045629.01.T01:intron | 45.0% | |
| GCCAGTACATTCTACTTTGG+AGG | + | Chr8:61965144-61965163 | None:intergenic | 45.0% | |
| GCGCCATTGCAACAATACAA+TGG | + | Chr8:61965437-61965456 | None:intergenic | 45.0% | |
| GCTGCCAGTACATTCTACTT+TGG | + | Chr8:61965147-61965166 | None:intergenic | 45.0% | |
| GGTCAGTTGAGCATAGTGTT+CGG | + | Chr8:61965082-61965101 | None:intergenic | 45.0% | |
| TAAGCATAGCTATAAGCGCG+TGG | + | Chr8:61965521-61965540 | None:intergenic | 45.0% | |
| TCCATTTGGGAGTGGACATT+TGG | - | Chr8:61965746-61965765 | MsG0880045629.01.T01:CDS | 45.0% | |
| TTGGTTCCACTGTTATGGGA+AGG | + | Chr8:61965262-61965281 | None:intergenic | 45.0% | |
| ! | AAAAGCGCGTGTGAAGCATT+TGG | - | Chr8:61965407-61965426 | MsG0880045629.01.T01:intron | 45.0% |
| ! | GATGCATGAGAATTTTCCGG+AGG | + | Chr8:61965105-61965124 | None:intergenic | 45.0% |
| ! | TGCATGAGAATTTTCCGGAG+GGG | + | Chr8:61965103-61965122 | None:intergenic | 45.0% |
| AAGGTGAAAATTGCGGCCGA+AGG | - | Chr8:61965353-61965372 | MsG0880045629.01.T01:intron | 50.0% | |
| ACTGCACAGCTAGCAACAAC+AGG | - | Chr8:61964987-61965006 | MsG0880045629.01.T01:CDS | 50.0% | |
| AGAGAGTAGTGAGAAGAGCG+TGG | - | Chr8:61965379-61965398 | MsG0880045629.01.T01:intron | 50.0% | |
| ATAGTGTTCGGCCCCATATG+CGG | + | Chr8:61965070-61965089 | None:intergenic | 50.0% | |
| CCCAAATGTCCACTCCCAAA+TGG | + | Chr8:61965750-61965769 | None:intergenic | 50.0% | |
| CCCATAACAGTGGAACCAAG+TGG | - | Chr8:61965263-61965282 | MsG0880045629.01.T01:intron | 50.0% | |
| GACAAAATTCCCGGCCAAAC+AGG | + | Chr8:61964957-61964976 | None:intergenic | 50.0% | |
| GCCACTTGGTTCCACTGTTA+TGG | + | Chr8:61965267-61965286 | None:intergenic | 50.0% | |
| GTTTAAGTCCACCAGACAGC+CGG | + | Chr8:61965312-61965331 | None:intergenic | 50.0% | |
| TCATGCATCACAGGAGACTG+TGG | - | Chr8:61965116-61965135 | MsG0880045629.01.T01:CDS | 50.0% | |
| TGTTGCTAGCTGTGCAGTTC+CGG | + | Chr8:61964985-61965004 | None:intergenic | 50.0% | |
| TTTGGATTCGCCCGGTTGTA+GGG | + | Chr8:61965018-61965037 | None:intergenic | 50.0% | |
| ! | AGAGGAACAAGTGAAGGTGC+TGG | + | Chr8:61965555-61965574 | None:intergenic | 50.0% |
| ! | GAAGGTTGTAACCGTCGACT+AGG | + | Chr8:61965244-61965263 | None:intergenic | 50.0% |
| ! | GTTTGGCCGGGAATTTTGTC+AGG | - | Chr8:61964957-61964976 | MsG0880045629.01.T01:CDS | 50.0% |
| !! | GGCTGTCTGGTGGACTTAAA+CGG | - | Chr8:61965311-61965330 | MsG0880045629.01.T01:intron | 50.0% |
| AACAGGCTTCGCCCTACAAC+CGG | - | Chr8:61965004-61965023 | MsG0880045629.01.T01:CDS | 55.0% | |
| ACCATGCCCACATCTTGGTC+CGG | - | Chr8:61965044-61965063 | MsG0880045629.01.T01:CDS | 55.0% | |
| ATCTTGGTCCGGCCGCATAT+GGG | - | Chr8:61965055-61965074 | MsG0880045629.01.T01:CDS | 55.0% | |
| CCGGCACCTGACAAAATTCC+CGG | + | Chr8:61964966-61964985 | None:intergenic | 55.0% | |
| CGCGTGTCCAACAGAACTGA+AGG | - | Chr8:61965334-61965353 | MsG0880045629.01.T01:intron | 55.0% | |
| CTATGCTCAACTGACCCCTC+CGG | - | Chr8:61965086-61965105 | MsG0880045629.01.T01:CDS | 55.0% | |
| CTGTCACCATGCCCACATCT+TGG | - | Chr8:61965039-61965058 | MsG0880045629.01.T01:CDS | 55.0% | |
| CTTTGGATTCGCCCGGTTGT+AGG | + | Chr8:61965019-61965038 | None:intergenic | 55.0% | |
| GGTGGAAACTGCACGACAAC+CGG | - | Chr8:61965290-61965309 | MsG0880045629.01.T01:intron | 55.0% | |
| GTGCAGCTATCCTGTTTGGC+CGG | - | Chr8:61964944-61964963 | MsG0880045629.01.T01:CDS | 55.0% | |
| TACGACGTCAGCCTAGTCGA+CGG | - | Chr8:61965230-61965249 | MsG0880045629.01.T01:intron | 55.0% | |
| TGCAGCTATCCTGTTTGGCC+GGG | - | Chr8:61964945-61964964 | MsG0880045629.01.T01:CDS | 55.0% | |
| TGTGGGCATGGTGACAGCTT+TGG | + | Chr8:61965036-61965055 | None:intergenic | 55.0% | |
| TTGGGAGTGGACATTTGGGC+AGG | - | Chr8:61965751-61965770 | MsG0880045629.01.T01:CDS | 55.0% | |
| ! | CCGGGAATTTTGTCAGGTGC+CGG | - | Chr8:61964963-61964982 | MsG0880045629.01.T01:CDS | 55.0% |
| !! | GTGACAGCTTTGGATTCGCC+CGG | + | Chr8:61965026-61965045 | None:intergenic | 55.0% |
| ACAGGCTTCGCCCTACAACC+GGG | - | Chr8:61965005-61965024 | MsG0880045629.01.T01:CDS | 60.0% | |
| ACAGTGGAACCAAGTGGCGG+CGG | - | Chr8:61965269-61965288 | MsG0880045629.01.T01:intron | 60.0% | |
| ATGCGGCCGGACCAAGATGT+GGG | + | Chr8:61965053-61965072 | None:intergenic | 60.0% | |
| CATCTTGGTCCGGCCGCATA+TGG | - | Chr8:61965054-61965073 | MsG0880045629.01.T01:CDS | 60.0% | |
| TATGCGGCCGGACCAAGATG+TGG | + | Chr8:61965054-61965073 | None:intergenic | 60.0% | |
| TCTTGGTCCGGCCGCATATG+GGG | - | Chr8:61965056-61965075 | MsG0880045629.01.T01:CDS | 60.0% | |
| CACGACAACCGGCTGTCTGG+TGG | - | Chr8:61965301-61965320 | MsG0880045629.01.T01:intron | 65.0% | |
| CAGTTTCCACCGCCGCCACT+TGG | + | Chr8:61965281-61965300 | None:intergenic | 65.0% | |
| CTGCACGACAACCGGCTGTC+TGG | - | Chr8:61965298-61965317 | MsG0880045629.01.T01:intron | 65.0% | |
| GCCGGACCAAGATGTGGGCA+TGG | + | Chr8:61965048-61965067 | None:intergenic | 65.0% | |
| TGTTCGGCCCCATATGCGGC+CGG | + | Chr8:61965066-61965085 | None:intergenic | 65.0% | |
| CATCCCTCCAGCGACGCTCG+CGG | - | Chr8:61965172-61965191 | MsG0880045629.01.T01:CDS | 70.0% | |
| GTGGAACCAAGTGGCGGCGG+TGG | - | Chr8:61965272-61965291 | MsG0880045629.01.T01:intron | 70.0% | |
| ! | AACTCCGCGAGCGTCGCTGG+AGG | + | Chr8:61965179-61965198 | None:intergenic | 70.0% |
| ! | ACTCCGCGAGCGTCGCTGGA+GGG | + | Chr8:61965178-61965197 | None:intergenic | 70.0% |
| ! | GTGAACTCCGCGAGCGTCGC+TGG | + | Chr8:61965182-61965201 | None:intergenic | 70.0% |
| ! | CGCGAGCGTCGCTGGAGGGA+TGG | + | Chr8:61965174-61965193 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 61964337 | 61965785 | 61964337 | ID=MsG0880045629.01;Name=MsG0880045629.01 |
| Chr8 | mRNA | 61964337 | 61965785 | 61964337 | ID=MsG0880045629.01.T01;Parent=MsG0880045629.01;Name=MsG0880045629.01.T01;_AED=0.33;_eAED=0.35;_QI=0|0|0|1|0|0|3|0|278 |
| Chr8 | exon | 61965722 | 61965785 | 61965722 | ID=MsG0880045629.01.T01:exon:12904;Parent=MsG0880045629.01.T01 |
| Chr8 | exon | 61964507 | 61965212 | 61964507 | ID=MsG0880045629.01.T01:exon:12903;Parent=MsG0880045629.01.T01 |
| Chr8 | exon | 61964337 | 61964403 | 61964337 | ID=MsG0880045629.01.T01:exon:12902;Parent=MsG0880045629.01.T01 |
| Chr8 | CDS | 61965722 | 61965785 | 61965722 | ID=MsG0880045629.01.T01:cds;Parent=MsG0880045629.01.T01 |
| Chr8 | CDS | 61964507 | 61965212 | 61964507 | ID=MsG0880045629.01.T01:cds;Parent=MsG0880045629.01.T01 |
| Chr8 | CDS | 61964337 | 61964403 | 61964337 | ID=MsG0880045629.01.T01:cds;Parent=MsG0880045629.01.T01 |
| Gene Sequence |
| Protein sequence |
