Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045714.01.T01 | XP_003629427.1 | 97.222 | 72 | 2 | 0 | 1 | 72 | 1 | 72 | 3.25E-45 | 149 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045714.01.T01 | F4JVB8 | 66.154 | 65 | 21 | 1 | 5 | 68 | 14 | 78 | 1.54E-27 | 98.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045714.01.T01 | G7LDY8 | 97.222 | 72 | 2 | 0 | 1 | 72 | 1 | 72 | 1.55e-45 | 149 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0880045709.01 | MsG0880045714.01 | 0.810351 | 1.203306e-50 | 7.236756e-48 |
| MsG0880045710.01 | MsG0880045714.01 | 0.875468 | 3.008459e-68 | 1.405997e-64 |
| MsG0880045711.01 | MsG0880045714.01 | 0.885083 | 1.104042e-71 | 7.472850e-68 |
| MsG0880045712.01 | MsG0880045714.01 | 0.861906 | 7.380613e-64 | 2.121269e-60 |
| MsG0880045713.01 | MsG0880045714.01 | 0.910426 | 1.898071e-82 | 3.873562e-78 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045714.01.T01 | MTR_8g077380 | 97.222 | 72 | 2 | 0 | 1 | 72 | 1 | 72 | 3.93e-49 | 149 |
| MsG0880045714.01.T01 | MTR_8g077390 | 97.222 | 72 | 2 | 0 | 1 | 72 | 1 | 72 | 3.93e-49 | 149 |
| MsG0880045714.01.T01 | MTR_8g077360 | 98.592 | 71 | 1 | 0 | 1 | 71 | 1 | 71 | 5.57e-49 | 149 |
| MsG0880045714.01.T01 | MTR_5g020540 | 82.258 | 62 | 11 | 0 | 3 | 64 | 2 | 63 | 1.40e-35 | 114 |
| MsG0880045714.01.T01 | MTR_8g077420 | 68.571 | 70 | 21 | 1 | 1 | 70 | 1 | 69 | 4.31e-32 | 106 |
| MsG0880045714.01.T01 | MTR_3g116720 | 64.615 | 65 | 22 | 1 | 5 | 68 | 16 | 80 | 1.46e-27 | 95.1 |
| MsG0880045714.01.T01 | MTR_1g022290 | 55.224 | 67 | 29 | 1 | 2 | 67 | 4 | 70 | 8.74e-26 | 90.9 |
| MsG0880045714.01.T01 | MTR_6g004250 | 57.576 | 66 | 27 | 1 | 2 | 66 | 7 | 72 | 3.67e-25 | 89.0 |
| MsG0880045714.01.T01 | MTR_5g020170 | 54.688 | 64 | 28 | 1 | 5 | 67 | 19 | 82 | 2.00e-24 | 87.8 |
| MsG0880045714.01.T01 | MTR_3g111880 | 55.072 | 69 | 30 | 1 | 2 | 69 | 4 | 72 | 1.00e-23 | 85.9 |
| MsG0880045714.01.T01 | MTR_3g013440 | 55.072 | 69 | 29 | 1 | 2 | 68 | 7 | 75 | 1.06e-22 | 83.2 |
| MsG0880045714.01.T01 | MTR_7g089210 | 59.016 | 61 | 24 | 1 | 8 | 67 | 15 | 75 | 5.61e-22 | 81.3 |
| MsG0880045714.01.T01 | MTR_5g069710 | 54.930 | 71 | 31 | 1 | 1 | 70 | 25 | 95 | 1.18e-20 | 82.8 |
| MsG0880045714.01.T01 | MTR_3g111920 | 52.174 | 69 | 32 | 1 | 4 | 71 | 2 | 70 | 6.96e-20 | 75.9 |
| MsG0880045714.01.T01 | MTR_3g462790 | 54.412 | 68 | 30 | 1 | 2 | 68 | 40 | 107 | 1.24e-19 | 80.1 |
| MsG0880045714.01.T01 | MTR_7g067080 | 51.389 | 72 | 34 | 1 | 2 | 72 | 32 | 103 | 7.37e-18 | 75.5 |
| MsG0880045714.01.T01 | MTR_8g063870 | 42.466 | 73 | 41 | 1 | 1 | 72 | 4 | 76 | 1.47e-16 | 71.2 |
| MsG0880045714.01.T01 | MTR_5g081860 | 47.887 | 71 | 36 | 1 | 3 | 72 | 25 | 95 | 4.50e-16 | 70.1 |
| MsG0880045714.01.T01 | MTR_1g033600 | 38.571 | 70 | 42 | 1 | 2 | 70 | 9 | 78 | 2.39e-15 | 65.9 |
| MsG0880045714.01.T01 | MTR_1g086180 | 47.143 | 70 | 35 | 1 | 3 | 70 | 9 | 78 | 4.16e-15 | 63.9 |
| MsG0880045714.01.T01 | MTR_7g110580 | 50.820 | 61 | 28 | 1 | 3 | 61 | 6 | 66 | 1.53e-14 | 62.4 |
| MsG0880045714.01.T01 | MTR_8g063600 | 40.000 | 65 | 38 | 1 | 5 | 68 | 10 | 74 | 3.87e-12 | 59.3 |
| MsG0880045714.01.T01 | MTR_1g111830 | 40.000 | 65 | 38 | 1 | 3 | 66 | 22 | 86 | 1.76e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045714.01.T01 | AT4G39250 | 66.154 | 65 | 21 | 1 | 5 | 68 | 14 | 78 | 1.57e-28 | 98.2 |
| MsG0880045714.01.T01 | AT1G75250 | 62.687 | 67 | 24 | 1 | 3 | 68 | 10 | 76 | 5.25e-27 | 94.4 |
| MsG0880045714.01.T01 | AT1G75250 | 64.615 | 65 | 22 | 1 | 5 | 68 | 12 | 76 | 8.10e-27 | 94.7 |
| MsG0880045714.01.T01 | AT2G21650 | 57.353 | 68 | 28 | 1 | 5 | 71 | 14 | 81 | 1.15e-25 | 90.9 |
| MsG0880045714.01.T01 | AT1G19510 | 61.290 | 62 | 23 | 1 | 8 | 68 | 15 | 76 | 7.94e-24 | 86.3 |
| MsG0880045714.01.T01 | AT2G18328 | 55.385 | 65 | 27 | 2 | 8 | 71 | 14 | 77 | 1.02e-18 | 72.8 |
| MsG0880045714.01.T01 | AT2G38090 | 51.389 | 72 | 34 | 1 | 2 | 72 | 26 | 97 | 2.36e-18 | 76.6 |
| MsG0880045714.01.T01 | AT5G58900 | 52.174 | 69 | 32 | 1 | 2 | 69 | 31 | 99 | 1.41e-17 | 74.3 |
| MsG0880045714.01.T01 | AT4G36570 | 65.909 | 44 | 14 | 1 | 5 | 47 | 12 | 55 | 7.38e-16 | 65.1 |
| MsG0880045714.01.T01 | AT5G05790 | 45.714 | 70 | 37 | 1 | 2 | 70 | 28 | 97 | 9.95e-16 | 69.3 |
| MsG0880045714.01.T01 | AT5G05790 | 45.714 | 70 | 37 | 1 | 2 | 70 | 28 | 97 | 9.95e-16 | 69.3 |
| MsG0880045714.01.T01 | AT3G11280 | 49.206 | 63 | 31 | 1 | 5 | 66 | 33 | 95 | 2.37e-15 | 68.2 |
| MsG0880045714.01.T01 | AT3G11280 | 49.206 | 63 | 31 | 1 | 5 | 66 | 33 | 95 | 2.37e-15 | 68.2 |
| MsG0880045714.01.T01 | AT5G08520 | 49.231 | 65 | 32 | 1 | 5 | 68 | 12 | 76 | 5.21e-14 | 64.7 |
| MsG0880045714.01.T01 | AT5G04760 | 43.284 | 67 | 37 | 1 | 1 | 66 | 1 | 67 | 3.15e-13 | 61.6 |
| MsG0880045714.01.T01 | AT1G49010 | 41.429 | 70 | 37 | 2 | 1 | 66 | 4 | 73 | 5.41e-12 | 59.3 |
Find 15 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAACATTGTGCCATCTTTC+TGG | 0.106622 | 8:-63155879 | None:intergenic |
| AAGCATTGGCTTTATATGAT+AGG | 0.319279 | 8:+63155850 | MsG0880045714.01.T01:CDS |
| TGTAACGAGGAAAAGGGATA+TGG | 0.417505 | 8:-63155983 | None:intergenic |
| TACGTTGAACATCTTCTTTG+AGG | 0.435409 | 8:-63155950 | None:intergenic |
| ATCAACAAATGGCAACCTCT+TGG | 0.450563 | 8:+63155799 | None:intergenic |
| CAAACTGTTTGAACAAGCAT+TGG | 0.467435 | 8:+63155836 | MsG0880045714.01.T01:CDS |
| AGCATTGGCTTTATATGATA+GGG | 0.471289 | 8:+63155851 | MsG0880045714.01.T01:CDS |
| TACGTATATACCTTAGGTGT+TGG | 0.532154 | 8:-63156015 | None:intergenic |
| CACAATGTTGCCAAAGTGGT+TGG | 0.534351 | 8:+63155891 | MsG0880045714.01.T01:CDS |
| AAAACTAATACCAACACCTA+AGG | 0.561974 | 8:+63156005 | MsG0880045714.01.T01:CDS |
| ATAGGGAAACTCCAGAAAGA+TGG | 0.571093 | 8:+63155868 | MsG0880045714.01.T01:CDS |
| TGGCAACCTCTTGGACTGCG+AGG | 0.629681 | 8:+63155808 | MsG0880045714.01.T01:CDS |
| GTTCTGCCTCGCAGTCCAAG+AGG | 0.630149 | 8:-63155814 | None:intergenic |
| ATGGCACAATGTTGCCAAAG+TGG | 0.642850 | 8:+63155887 | MsG0880045714.01.T01:CDS |
| GATGTTCAACGTATTGAGCG+TGG | 0.669371 | 8:+63155960 | MsG0880045714.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGCATTGGCTTTATATGAT+AGG | + | Chr8:63155850-63155869 | MsG0880045714.01.T01:CDS | 30.0% |
| !! | AGCATTGGCTTTATATGATA+GGG | + | Chr8:63155851-63155870 | MsG0880045714.01.T01:CDS | 30.0% |
| !!! | TAGTTTTGTAACGAGGAAAA+GGG | - | Chr8:63155992-63156011 | None:intergenic | 30.0% |
| !!! | TTAGTTTTGTAACGAGGAAA+AGG | - | Chr8:63155993-63156012 | None:intergenic | 30.0% |
| !!! | TTGGTATTAGTTTTGTAACG+AGG | - | Chr8:63155999-63156018 | None:intergenic | 30.0% |
| CAAACTGTTTGAACAAGCAT+TGG | + | Chr8:63155836-63155855 | MsG0880045714.01.T01:CDS | 35.0% | |
| TACGTTGAACATCTTCTTTG+AGG | - | Chr8:63155953-63155972 | None:intergenic | 35.0% | |
| !! | AACTGATTTTCCAACCACTT+TGG | - | Chr8:63155904-63155923 | None:intergenic | 35.0% |
| ATAGGGAAACTCCAGAAAGA+TGG | + | Chr8:63155868-63155887 | MsG0880045714.01.T01:CDS | 40.0% | |
| TGTAACGAGGAAAAGGGATA+TGG | - | Chr8:63155986-63156005 | None:intergenic | 40.0% | |
| ATGGCACAATGTTGCCAAAG+TGG | + | Chr8:63155887-63155906 | MsG0880045714.01.T01:CDS | 45.0% | |
| CACAATGTTGCCAAAGTGGT+TGG | + | Chr8:63155891-63155910 | MsG0880045714.01.T01:CDS | 45.0% | |
| GATGTTCAACGTATTGAGCG+TGG | + | Chr8:63155960-63155979 | MsG0880045714.01.T01:CDS | 45.0% | |
| GCAACATTGTGCCATCTTTC+TGG | - | Chr8:63155882-63155901 | None:intergenic | 45.0% | |
| GTTCTGCCTCGCAGTCCAAG+AGG | - | Chr8:63155817-63155836 | None:intergenic | 60.0% | |
| TGGCAACCTCTTGGACTGCG+AGG | + | Chr8:63155808-63155827 | MsG0880045714.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 63155807 | 63156025 | 63155807 | ID=MsG0880045714.01;Name=MsG0880045714.01 |
| Chr8 | mRNA | 63155807 | 63156025 | 63155807 | ID=MsG0880045714.01.T01;Parent=MsG0880045714.01;Name=MsG0880045714.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|72 |
| Chr8 | exon | 63155807 | 63156025 | 63155807 | ID=MsG0880045714.01.T01:exon:24119;Parent=MsG0880045714.01.T01 |
| Chr8 | CDS | 63155807 | 63156025 | 63155807 | ID=MsG0880045714.01.T01:cds;Parent=MsG0880045714.01.T01 |
| Gene Sequence |
| Protein sequence |
