AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045710.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045710.01.T01 MTR_8g077380 98.611 72 1 0 1 72 1 72 1.40e-49 150
MsG0880045710.01.T01 MTR_8g077390 98.611 72 1 0 1 72 1 72 1.40e-49 150
MsG0880045710.01.T01 MTR_8g077360 97.222 72 2 0 1 72 1 72 4.42e-49 149
MsG0880045710.01.T01 MTR_5g020540 82.258 62 11 0 3 64 2 63 1.40e-35 114
MsG0880045710.01.T01 MTR_8g077420 68.571 70 21 1 1 70 1 69 4.08e-32 106
MsG0880045710.01.T01 MTR_3g116720 64.615 65 22 1 5 68 16 80 1.48e-27 95.1
MsG0880045710.01.T01 MTR_1g022290 55.224 67 29 1 2 67 4 70 8.09e-26 91.3
MsG0880045710.01.T01 MTR_6g004250 57.576 66 27 1 2 66 7 72 3.59e-25 89.0
MsG0880045710.01.T01 MTR_5g020170 54.688 64 28 1 5 67 19 82 1.82e-24 87.8
MsG0880045710.01.T01 MTR_3g111880 55.072 69 30 1 2 69 4 72 8.41e-24 85.9
MsG0880045710.01.T01 MTR_3g013440 55.072 69 29 1 2 68 7 75 1.12e-22 83.2
MsG0880045710.01.T01 MTR_7g089210 59.016 61 24 1 8 67 15 75 5.14e-22 81.3
MsG0880045710.01.T01 MTR_5g069710 54.930 71 31 1 1 70 25 95 1.01e-20 82.8
MsG0880045710.01.T01 MTR_3g111920 52.174 69 32 1 4 71 2 70 6.67e-20 75.9
MsG0880045710.01.T01 MTR_3g462790 54.412 68 30 1 2 68 40 107 1.14e-19 80.1
MsG0880045710.01.T01 MTR_7g067080 51.429 70 33 1 2 70 32 101 9.42e-18 75.1
MsG0880045710.01.T01 MTR_8g063870 43.836 73 40 1 1 72 4 76 7.22e-17 72.0
MsG0880045710.01.T01 MTR_5g081860 50.769 65 31 1 3 66 25 89 6.80e-16 69.7
MsG0880045710.01.T01 MTR_1g033600 38.571 70 42 1 2 70 9 78 2.58e-15 65.9
MsG0880045710.01.T01 MTR_1g086180 47.143 70 35 1 3 70 9 78 4.35e-15 63.9
MsG0880045710.01.T01 MTR_7g110580 50.820 61 28 1 3 61 6 66 1.58e-14 62.4
MsG0880045710.01.T01 MTR_8g063600 39.130 69 41 1 5 72 10 78 2.89e-12 59.7
MsG0880045710.01.T01 MTR_1g111830 40.000 65 38 1 3 66 22 86 1.74e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045710.01.T01 AT4G39250 66.154 65 21 1 5 68 14 78 1.41e-28 98.2
MsG0880045710.01.T01 AT1G75250 62.687 67 24 1 3 68 10 76 4.76e-27 94.4
MsG0880045710.01.T01 AT1G75250 64.615 65 22 1 5 68 12 76 6.58e-27 94.7
MsG0880045710.01.T01 AT2G21650 56.522 69 29 1 5 72 14 82 8.40e-26 91.3
MsG0880045710.01.T01 AT1G19510 61.290 62 23 1 8 68 15 76 7.35e-24 86.3
MsG0880045710.01.T01 AT2G18328 55.385 65 27 2 8 71 14 77 1.04e-18 72.8
MsG0880045710.01.T01 AT2G38090 51.429 70 33 1 2 70 26 95 3.22e-18 76.3
MsG0880045710.01.T01 AT5G58900 52.174 69 32 1 2 69 31 99 1.33e-17 74.3
MsG0880045710.01.T01 AT4G36570 65.909 44 14 1 5 47 12 55 6.83e-16 65.1
MsG0880045710.01.T01 AT5G05790 45.714 70 37 1 2 70 28 97 9.26e-16 69.3
MsG0880045710.01.T01 AT5G05790 45.714 70 37 1 2 70 28 97 9.26e-16 69.3
MsG0880045710.01.T01 AT3G11280 49.206 63 31 1 5 66 33 95 2.42e-15 68.2
MsG0880045710.01.T01 AT3G11280 49.206 63 31 1 5 66 33 95 2.42e-15 68.2
MsG0880045710.01.T01 AT5G08520 47.826 69 35 1 5 72 12 80 3.62e-14 65.1
MsG0880045710.01.T01 AT5G04760 43.284 67 37 1 1 66 1 67 3.29e-13 61.6
MsG0880045710.01.T01 AT5G01200 45.070 71 37 2 4 72 29 99 1.61e-12 60.5
MsG0880045710.01.T01 AT1G49010 41.429 70 37 2 1 66 4 73 5.46e-12 59.3

Find 13 sgRNAs with CRISPR-Local

Find 14 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAACATTGTGCCATCTTTC+TGG 0.106622 8:-63101567 None:intergenic
TGCGTTGAACATCTTCTTTG+AGG 0.413444 8:-63101638 None:intergenic
TGTAACGAGGAAAAGGGATA+TGG 0.417505 8:-63101671 None:intergenic
CAAACTGTTTGAACAAGCAT+TGG 0.467435 8:+63101524 MsG0880045710.01.T01:CDS
CACAATGTTGCCAAAGTGGT+TGG 0.534351 8:+63101579 MsG0880045710.01.T01:CDS
ATCAACAAATGGCAACCTCA+TGG 0.544947 8:+63101487 None:intergenic
AAAACTAACACCAACTCCTA+AGG 0.547909 8:+63101693 MsG0880045710.01.T01:CDS
ATAGAGAAACTCCAGAAAGA+TGG 0.558722 8:+63101556 MsG0880045710.01.T01:CDS
ATATATTATACCTTAGGAGT+TGG 0.560505 8:-63101703 None:intergenic
ATGGCACAATGTTGCCAAAG+TGG 0.642850 8:+63101575 MsG0880045710.01.T01:CDS
GATGTTCAACGCATTGAGCG+TGG 0.644275 8:+63101648 MsG0880045710.01.T01:CDS
TGGCAACCTCATGGACTGCG+AGG 0.652849 8:+63101496 MsG0880045710.01.T01:CDS
GTTCTGCCTCGCAGTCCATG+AGG 0.665063 8:-63101502 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAGTTTTGTAACGAGGAAAA+GGG - Chr8:63101680-63101699 None:intergenic 30.0%
!!! TTAGTTTTGTAACGAGGAAA+AGG - Chr8:63101681-63101700 None:intergenic 30.0%
ATAGAGAAACTCCAGAAAGA+TGG + Chr8:63101556-63101575 MsG0880045710.01.T01:CDS 35.0%
CAAACTGTTTGAACAAGCAT+TGG + Chr8:63101524-63101543 MsG0880045710.01.T01:CDS 35.0%
!! AACTGATTTTCCAACCACTT+TGG - Chr8:63101592-63101611 None:intergenic 35.0%
!!! TTGGTGTTAGTTTTGTAACG+AGG - Chr8:63101687-63101706 None:intergenic 35.0%
TGCGTTGAACATCTTCTTTG+AGG - Chr8:63101641-63101660 None:intergenic 40.0%
TGTAACGAGGAAAAGGGATA+TGG - Chr8:63101674-63101693 None:intergenic 40.0%
ATGGCACAATGTTGCCAAAG+TGG + Chr8:63101575-63101594 MsG0880045710.01.T01:CDS 45.0%
CACAATGTTGCCAAAGTGGT+TGG + Chr8:63101579-63101598 MsG0880045710.01.T01:CDS 45.0%
GCAACATTGTGCCATCTTTC+TGG - Chr8:63101570-63101589 None:intergenic 45.0%
GATGTTCAACGCATTGAGCG+TGG + Chr8:63101648-63101667 MsG0880045710.01.T01:CDS 50.0%
GTTCTGCCTCGCAGTCCATG+AGG - Chr8:63101505-63101524 None:intergenic 60.0%
! TGGCAACCTCATGGACTGCG+AGG + Chr8:63101496-63101515 MsG0880045710.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 63101495 63101713 63101495 ID=MsG0880045710.01;Name=MsG0880045710.01
Chr8 mRNA 63101495 63101713 63101495 ID=MsG0880045710.01.T01;Parent=MsG0880045710.01;Name=MsG0880045710.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|72
Chr8 exon 63101495 63101713 63101495 ID=MsG0880045710.01.T01:exon:24110;Parent=MsG0880045710.01.T01
Chr8 CDS 63101495 63101713 63101495 ID=MsG0880045710.01.T01:cds;Parent=MsG0880045710.01.T01
Gene Sequence

>MsG0880045710.01.T01

ATGGCAACCTCATGGACTGCGAGGCAGAACAAACTGTTTGAACAAGCATTGGCTTTATATGATAGAGAAACTCCAGAAAGATGGCACAATGTTGCCAAAGTGGTTGGAAAATCAGTTGAAGATGTTAAGAGTCACTATGAGATCCTCAAAGAAGATGTTCAACGCATTGAGCGTGGCCATATCCCTTTTCCTCGTTACAAAACTAACACCAACTCCTAA

Protein sequence

>MsG0880045710.01.T01

MATSWTARQNKLFEQALALYDRETPERWHNVAKVVGKSVEDVKSHYEILKEDVQRIERGHIPFPRYKTNTNS*