AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880046685.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046685.01.T01 MTR_4g113020 95.294 510 5 1 1 491 1 510 0.0 993
MsG0880046685.01.T01 MTR_4g113020 99.458 369 2 0 1 369 1 369 0.0 758
MsG0880046685.01.T01 MTR_1g011460 57.685 501 177 7 1 478 1 489 0.0 599
MsG0880046685.01.T01 MTR_3g107980 56.827 498 182 7 1 472 1 491 0.0 583
MsG0880046685.01.T01 MTR_6g022720 50.598 502 201 6 1 464 1 493 7.20e-174 499
MsG0880046685.01.T01 MTR_3g080070 48.976 537 223 5 1 489 1 534 1.22e-172 497
MsG0880046685.01.T01 MTR_3g086010 49.000 500 220 5 1 465 1 500 1.58e-169 488
MsG0880046685.01.T01 MTR_1g062950 51.374 473 197 7 15 465 15 476 1.19e-165 477
MsG0880046685.01.T01 MTR_1g111570 46.721 488 221 6 5 465 1 476 2.54e-157 456
MsG0880046685.01.T01 MTR_6g022720 54.264 387 149 5 1 368 1 378 2.17e-143 417
MsG0880046685.01.T01 MTR_7g102900 51.524 361 152 6 11 368 3 343 8.30e-129 379
MsG0880046685.01.T01 MTR_1g112940 38.717 421 230 5 82 478 31 447 6.88e-103 315
MsG0880046685.01.T01 MTR_7g102920 50.442 113 51 1 365 477 19 126 2.19e-31 117
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046685.01.T01 AT5G25510 59.486 506 180 4 1 487 1 500 0.0 626
MsG0880046685.01.T01 AT4G15415 52.121 495 208 5 1 466 1 495 9.44e-179 512
MsG0880046685.01.T01 AT4G15415 52.121 495 208 5 1 466 1 495 9.44e-179 512
MsG0880046685.01.T01 AT4G15415 52.121 495 208 5 1 466 1 495 9.44e-179 512
MsG0880046685.01.T01 AT4G15415 52.121 495 208 5 1 466 1 495 9.44e-179 512
MsG0880046685.01.T01 AT3G26020 51.953 512 187 6 1 462 1 503 1.10e-176 506
MsG0880046685.01.T01 AT3G26020 51.953 512 187 6 1 462 1 503 1.10e-176 506
MsG0880046685.01.T01 AT3G26020 51.953 512 187 6 1 462 1 503 1.10e-176 506
MsG0880046685.01.T01 AT3G26020 51.953 512 187 6 1 462 1 503 1.10e-176 506
MsG0880046685.01.T01 AT1G13460 57.971 414 152 2 77 468 75 488 2.53e-176 504
MsG0880046685.01.T01 AT1G13460 57.971 414 152 2 77 468 75 488 2.53e-176 504
MsG0880046685.01.T01 AT3G21650 50.962 520 197 6 1 466 1 516 1.53e-175 504
MsG0880046685.01.T01 AT3G21650 50.962 520 197 6 1 466 1 516 1.53e-175 504
MsG0880046685.01.T01 AT3G09880 50.302 497 220 4 1 475 1 492 7.26e-169 486
MsG0880046685.01.T01 AT5G03470 49.180 488 217 5 1 463 1 482 1.52e-168 485
MsG0880046685.01.T01 AT3G26030 48.276 493 211 10 1 467 1 475 1.69e-154 448
MsG0880046685.01.T01 AT3G26020 45.488 543 177 7 1 462 1 505 9.67e-149 435
MsG0880046685.01.T01 AT3G26020 45.488 543 177 7 1 462 1 505 9.67e-149 435
MsG0880046685.01.T01 AT3G26020 45.488 543 177 7 1 462 1 505 9.67e-149 435
MsG0880046685.01.T01 AT3G54930 49.515 412 184 2 78 465 82 493 6.06e-144 422
MsG0880046685.01.T01 AT3G54930 49.515 412 184 2 78 465 82 493 6.06e-144 422
MsG0880046685.01.T01 AT4G15415 55.291 378 159 4 1 368 1 378 1.04e-142 417
MsG0880046685.01.T01 AT3G26020 40.179 560 199 12 1 462 1 522 3.31e-115 350
MsG0880046685.01.T01 AT3G54930 52.027 296 137 1 78 368 82 377 4.45e-106 322

Find 114 sgRNAs with CRISPR-Local

Find 171 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATGACACTCTTGCATATT+AGG 0.187734 8:-76755681 None:intergenic
CTAACAGATTTCCTTATAAA+AGG 0.202511 8:-76753871 None:intergenic
GAATCTGATGAGTCGGATTT+TGG 0.220632 8:-76753181 None:intergenic
ACAGTTCTGCATAGAATCTA+TGG 0.252421 8:+76753829 MsG0880046685.01.T01:CDS
GGCAATAGTACTACTAGTTT+TGG 0.252801 8:+76753220 MsG0880046685.01.T01:CDS
TAATGGAATTGCTGAGCTTT+TGG 0.252902 8:+76753933 MsG0880046685.01.T01:CDS
ATGATAAATACCAATAGATT+TGG 0.253333 8:-76754049 None:intergenic
GGTTCAACTTACTGATAAAA+AGG 0.257384 8:-76753405 None:intergenic
GAGTGAGTTGGAAGAGGTTT+TGG 0.272535 8:+76754194 MsG0880046685.01.T01:CDS
GTGTGCAACCAATTTGTTTA+GGG 0.286986 8:+76753576 MsG0880046685.01.T01:CDS
TGTAAATTTCACTTGGCATT+AGG 0.291206 8:-76755774 None:intergenic
TGTTCTGTACAGTTCTTATC+AGG 0.296622 8:-76753457 None:intergenic
AACATTTAATTTGCTTGAAA+TGG 0.301664 8:-76753275 None:intergenic
TCATGAACATTAGCTCCTTT+TGG 0.304127 8:-76754173 None:intergenic
TTATGTTCTGAGTCAGGTTC+AGG 0.305779 8:-76755049 None:intergenic
ATTGGTTAGCTCAGTGATAA+AGG 0.320886 8:+76754119 MsG0880046685.01.T01:CDS
TGTGTGCAACCAATTTGTTT+AGG 0.324252 8:+76753575 MsG0880046685.01.T01:CDS
CGCTGGTGGCGGCTTCTTCA+AGG 0.337496 8:-76755187 None:intergenic
GATTCTGCAAAGAGTGATTC+TGG 0.347501 8:+76753199 MsG0880046685.01.T01:CDS
ACACCAGCATTCATGCTTGC+AGG 0.350797 8:-76753325 None:intergenic
TTGAAGATAGCACTGGCAAT+AGG 0.363955 8:+76755373 MsG0880046685.01.T01:three_prime_UTR
CGGAACGAGCTCATTTGTTA+TGG 0.366378 8:+76754919 MsG0880046685.01.T01:CDS
TTGCACAAACCCAAATCTAT+TGG 0.370970 8:+76754039 MsG0880046685.01.T01:CDS
CACTGTAACCTGTTTCCTAA+TGG 0.370976 8:-76755524 None:intergenic
TGGAGGATCCATTAGGAAAC+AGG 0.371223 8:+76755516 MsG0880046685.01.T01:three_prime_UTR
TCTTTCTTATGTTCTGAGTC+AGG 0.372770 8:-76755055 None:intergenic
AACTGAACAAAAGGTGAAAC+AGG 0.374985 8:-76755628 None:intergenic
GCAAATTAAATGTTGTGAAA+AGG 0.378108 8:+76753284 MsG0880046685.01.T01:CDS
TGGGAAAACCCTAAACAAAT+TGG 0.379876 8:-76753584 None:intergenic
TGATAAATACCAATAGATTT+GGG 0.382190 8:-76754048 None:intergenic
AACAGGTTACAGTGGTGGTG+GGG 0.397837 8:+76755533 MsG0880046685.01.T01:three_prime_UTR
ATGGGAAATTCATGGTTCAT+AGG 0.411237 8:+76753848 MsG0880046685.01.T01:CDS
GTAGATCATAAACAACTTGC+AGG 0.412759 8:-76753678 None:intergenic
CAAATTAAATGTTGTGAAAA+GGG 0.416656 8:+76753285 MsG0880046685.01.T01:CDS
ACAGGTTACAGTGGTGGTGG+GGG 0.422448 8:+76755534 MsG0880046685.01.T01:three_prime_UTR
CATAGATAAGGATCAAAGAT+TGG 0.423129 8:+76754101 MsG0880046685.01.T01:CDS
TTTGAAACCGAGCGGCATAA+TGG 0.423693 8:+76753916 MsG0880046685.01.T01:CDS
TAATATGCTATTACATTCAC+TGG 0.429069 8:+76755705 MsG0880046685.01.T01:three_prime_UTR
TTCAAACAATCTCTTTCTCT+AGG 0.433235 8:-76753805 None:intergenic
TAGGGTCTTCAGAATCAAAA+AGG 0.434877 8:-76753786 None:intergenic
AAGAAAGATGCTATCTCAAA+TGG 0.436356 8:+76755070 MsG0880046685.01.T01:CDS
CATTGTACAGTTCATAGATA+AGG 0.447391 8:+76754089 MsG0880046685.01.T01:CDS
TTACACAACGCAGCGATTGC+TGG 0.448320 8:-76753550 None:intergenic
CAACAAGGTCTAACAACGTT+TGG 0.456235 8:-76753492 None:intergenic
AGCCTGTGTTTGATCCTGCT+TGG 0.461055 8:+76753650 MsG0880046685.01.T01:CDS
CAGGTTACAGTGGTGGTGGG+GGG 0.463589 8:+76755535 MsG0880046685.01.T01:three_prime_UTR
TTGAATGATTTGTAAGAGGT+TGG 0.469927 8:+76755311 MsG0880046685.01.T01:three_prime_UTR
TATCATAAACTTTGCAGCAA+AGG 0.472991 8:-76753426 None:intergenic
CACCGCCACCGCCTACAGAT+TGG 0.473958 8:-76755211 None:intergenic
TTAAAATTTGAAGATAGCAC+TGG 0.475257 8:+76755366 MsG0880046685.01.T01:three_prime_UTR
TAAAGGGACTATTGAAGTAT+TGG 0.483354 8:+76754136 MsG0880046685.01.T01:CDS
TGAAGATAGCACTGGCAATA+GGG 0.484431 8:+76755374 MsG0880046685.01.T01:three_prime_UTR
CCACTACTCGAACGAAACTT+AGG 0.485337 8:-76753607 None:intergenic
TTGGTTAGCTCAGTGATAAA+GGG 0.485661 8:+76754120 MsG0880046685.01.T01:CDS
GTCCAATCTGTAGGCGGTGG+CGG 0.487249 8:+76755209 MsG0880046685.01.T01:CDS
GTGCTCTTCTTTCAATGGCA+AGG 0.488424 8:-76753986 None:intergenic
CTTCCTCTTCGATCTTGCGT+TGG 0.492096 8:-76755115 None:intergenic
AGGTTACAGTGGTGGTGGGG+GGG 0.494447 8:+76755536 MsG0880046685.01.T01:three_prime_UTR
TAGCAGTAGCTGGTTTCACT+TGG 0.494863 8:-76755244 None:intergenic
ACCGAGCATGTAGCAGTAGC+TGG 0.494955 8:-76755254 None:intergenic
TAGAATCTATGGGAAATTCA+TGG 0.495390 8:+76753840 MsG0880046685.01.T01:CDS
GTTGGTTCTAATTTACATAG+TGG 0.496428 8:+76755329 MsG0880046685.01.T01:three_prime_UTR
TACATAACTCACAGAATCAC+TGG 0.498799 8:+76755015 MsG0880046685.01.T01:CDS
TTGCTTAGGTGGTGCTGAGT+AGG 0.510457 8:+76755476 MsG0880046685.01.T01:three_prime_UTR
CGAGAGGCAGAATCACTTGT+CGG 0.511926 8:-76754977 None:intergenic
CCTAAGTTTCGTTCGAGTAG+TGG 0.514270 8:+76753607 MsG0880046685.01.T01:CDS
GTATTTCAGTGTACAAATGT+TGG 0.518089 8:+76753247 MsG0880046685.01.T01:CDS
GTTCATGAGTGAGTTGGAAG+AGG 0.521832 8:+76754188 MsG0880046685.01.T01:CDS
ATAGATTTGGGTTTGTGCAA+AGG 0.523747 8:-76754036 None:intergenic
TCAAACAATCTCTTTCTCTA+GGG 0.524186 8:-76753804 None:intergenic
GTTGTTGAATGATTTGTAAG+AGG 0.531556 8:+76755307 MsG0880046685.01.T01:three_prime_UTR
GAAACAGGTTACAGTGGTGG+TGG 0.531817 8:+76755531 MsG0880046685.01.T01:three_prime_UTR
CTGTTAGCAATATCATCTAC+CGG 0.536734 8:+76753887 MsG0880046685.01.T01:CDS
AGCAATTCCATTATGCCGCT+CGG 0.539353 8:-76753923 None:intergenic
GCCAGTTACTAATAGCCAAA+AGG 0.540094 8:+76754158 MsG0880046685.01.T01:CDS
AGCGTCCAATCTGTAGGCGG+TGG 0.544034 8:+76755206 MsG0880046685.01.T01:CDS
TTTCCTGCAAGCATGAATGC+TGG 0.544053 8:+76753322 MsG0880046685.01.T01:CDS
AAACAGGTTACAGTGGTGGT+GGG 0.544991 8:+76755532 MsG0880046685.01.T01:three_prime_UTR
ACCAGCTACTGCTACATGCT+CGG 0.547168 8:+76755253 MsG0880046685.01.T01:CDS
CTTTGCAGAATCTGATGAGT+CGG 0.547663 8:-76753188 None:intergenic
TACAGATTGGACGCTGGTGG+CGG 0.549605 8:-76755198 None:intergenic
TGACACTCTTGCATATTAGG+AGG 0.551807 8:-76755678 None:intergenic
GCTAATGTTCATGAGTGAGT+TGG 0.552985 8:+76754182 MsG0880046685.01.T01:CDS
CTGAGAGGCGAAGAATAACA+TGG 0.555313 8:+76755159 MsG0880046685.01.T01:CDS
ACATACATGTAAATTTCACT+TGG 0.567541 8:-76755781 None:intergenic
CAGTTCTGCATAGAATCTAT+GGG 0.572378 8:+76753830 MsG0880046685.01.T01:CDS
AACATCTTTAAACGATAGAG+AGG 0.586528 8:-76753362 None:intergenic
AAGTTTCGTTCGAGTAGTGG+TGG 0.590711 8:+76753610 MsG0880046685.01.T01:CDS
ACATCTTTAAACGATAGAGA+GGG 0.592218 8:-76753361 None:intergenic
CTACTCGAACGAAACTTAGG+TGG 0.596175 8:-76753604 None:intergenic
GACACTCTTGCATATTAGGA+GGG 0.600899 8:-76755677 None:intergenic
ATATTAGGAGGGAAAAGTGT+GGG 0.613841 8:-76755666 None:intergenic
ACTGGTTACTATATGCTATG+TGG 0.618468 8:+76755723 MsG0880046685.01.T01:three_prime_UTR
ACTTGCAGGTGAGACCAAGC+AGG 0.619708 8:-76753664 None:intergenic
TACTCGAACGAAACTTAGGT+GGG 0.627591 8:-76753603 None:intergenic
AACAAAAGCATGATCTACGT+GGG 0.634366 8:-76753749 None:intergenic
AAACAAAAGCATGATCTACG+TGG 0.636662 8:-76753750 None:intergenic
GCCACCAGCGTCCAATCTGT+AGG 0.637726 8:+76755200 MsG0880046685.01.T01:CDS
GCCTACAGATTGGACGCTGG+TGG 0.638027 8:-76755201 None:intergenic
AGGGAAAAGTGTGGGCCATG+AGG 0.654784 8:-76755658 None:intergenic
CATATTAGGAGGGAAAAGTG+TGG 0.657538 8:-76755667 None:intergenic
GATGCTATCTCAAATGGACG+AGG 0.659273 8:+76755076 MsG0880046685.01.T01:CDS
TAGGAAACAGGTTACAGTGG+TGG 0.662595 8:+76755528 MsG0880046685.01.T01:three_prime_UTR
TAGCATGGCTGAATTCCAGA+AGG 0.663321 8:+76754224 MsG0880046685.01.T01:CDS
CAGATGAAACAAAATCAACA+AGG 0.673447 8:-76753507 None:intergenic
ATGCCAACGCAAGATCGAAG+AGG 0.677288 8:+76755112 MsG0880046685.01.T01:CDS
ACCAGCGTCCAATCTGTAGG+CGG 0.680057 8:+76755203 MsG0880046685.01.T01:CDS
CATTAGGAAACAGGTTACAG+TGG 0.682100 8:+76755525 MsG0880046685.01.T01:three_prime_UTR
CATCTTTAAACGATAGAGAG+GGG 0.686542 8:-76753360 None:intergenic
TGAGAGGCGAAGAATAACAT+GGG 0.696192 8:+76755160 MsG0880046685.01.T01:CDS
GACCAAGCAGGATCAAACAC+AGG 0.696221 8:-76753652 None:intergenic
ACCGCCTACAGATTGGACGC+TGG 0.697587 8:-76755204 None:intergenic
ACAAGTGCTGAGAACACGAG+AGG 0.732134 8:-76754993 None:intergenic
AATCAAGTGATGTAGCTGAG+AGG 0.735001 8:+76755144 MsG0880046685.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTATAACAAAAGATAAAAT+AGG - Chr8:76754576-76754595 None:intergenic 15.0%
!! ATGATAAATACCAATAGATT+TGG - Chr8:76754052-76754071 None:intergenic 20.0%
!! CAAATTAAATGTTGTGAAAA+GGG + Chr8:76753285-76753304 MsG0880046685.01.T01:CDS 20.0%
!! TGATAAATACCAATAGATTT+GGG - Chr8:76754051-76754070 None:intergenic 20.0%
!! TTAAGTAACTATAAATTGCA+AGG - Chr8:76754823-76754842 None:intergenic 20.0%
!!! AAACAGCTTATAACTTTTTA+TGG + Chr8:76754538-76754557 MsG0880046685.01.T01:intron 20.0%
!!! AACATTTAATTTGCTTGAAA+TGG - Chr8:76753278-76753297 None:intergenic 20.0%
! AACAACTTATGAAATGTCAT+AGG - Chr8:76754479-76754498 None:intergenic 25.0%
! CATATGAAATGCTTAGATAT+TGG - Chr8:76754788-76754807 None:intergenic 25.0%
! CTAACAGATTTCCTTATAAA+AGG - Chr8:76753874-76753893 None:intergenic 25.0%
! GCAAATTAAATGTTGTGAAA+AGG + Chr8:76753284-76753303 MsG0880046685.01.T01:CDS 25.0%
! TAATATGCTATTACATTCAC+TGG + Chr8:76755705-76755724 MsG0880046685.01.T01:three_prime_UTR 25.0%
! TTAAAATTTGAAGATAGCAC+TGG + Chr8:76755366-76755385 MsG0880046685.01.T01:three_prime_UTR 25.0%
!! ATATTTTCTTGTGATTGTTC+AGG + Chr8:76754893-76754912 MsG0880046685.01.T01:intron 25.0%
!! CAAAAACAACACAATTTTGA+AGG - Chr8:76755459-76755478 None:intergenic 25.0%
!!! AAATTGTGTTGTTTTTGCTT+AGG + Chr8:76755462-76755481 MsG0880046685.01.T01:three_prime_UTR 25.0%
!!! AAGAGCACAAAATATTTTTG+TGG + Chr8:76754001-76754020 MsG0880046685.01.T01:CDS 25.0%
AACAAACTGTTTATCCAAAC+TGG + Chr8:76754652-76754671 MsG0880046685.01.T01:intron 30.0%
AACATCTTTAAACGATAGAG+AGG - Chr8:76753365-76753384 None:intergenic 30.0%
AAGAAAGATGCTATCTCAAA+TGG + Chr8:76755070-76755089 MsG0880046685.01.T01:CDS 30.0%
AAGAACTTATGAGACTGTTT+GGG + Chr8:76754436-76754455 MsG0880046685.01.T01:intron 30.0%
ACATCTTTAAACGATAGAGA+GGG - Chr8:76753364-76753383 None:intergenic 30.0%
CAAACAGATACTAATGTACA+AGG - Chr8:76754385-76754404 None:intergenic 30.0%
CAGATGAAACAAAATCAACA+AGG - Chr8:76753510-76753529 None:intergenic 30.0%
CATAGATAAGGATCAAAGAT+TGG + Chr8:76754101-76754120 MsG0880046685.01.T01:CDS 30.0%
CATTGTACAGTTCATAGATA+AGG + Chr8:76754089-76754108 MsG0880046685.01.T01:CDS 30.0%
CTTGTACATTAGTATCTGTT+TGG + Chr8:76754383-76754402 MsG0880046685.01.T01:intron 30.0%
GGTAACAAAAACTGAACAAA+AGG - Chr8:76755640-76755659 None:intergenic 30.0%
GGTTCAACTTACTGATAAAA+AGG - Chr8:76753408-76753427 None:intergenic 30.0%
GTATTTCAGTGTACAAATGT+TGG + Chr8:76753247-76753266 MsG0880046685.01.T01:CDS 30.0%
TAAAGGGACTATTGAAGTAT+TGG + Chr8:76754136-76754155 MsG0880046685.01.T01:CDS 30.0%
TAAGAACTTATGAGACTGTT+TGG + Chr8:76754435-76754454 MsG0880046685.01.T01:intron 30.0%
TAGAATCTATGGGAAATTCA+TGG + Chr8:76753840-76753859 MsG0880046685.01.T01:CDS 30.0%
TATCATAAACTTTGCAGCAA+AGG - Chr8:76753429-76753448 None:intergenic 30.0%
TCAAACAATCTCTTTCTCTA+GGG - Chr8:76753807-76753826 None:intergenic 30.0%
TGAGGATATTGGATTACAAA+TGG + Chr8:76754682-76754701 MsG0880046685.01.T01:intron 30.0%
TGTAAATTTCACTTGGCATT+AGG - Chr8:76755777-76755796 None:intergenic 30.0%
TTCAAACAATCTCTTTCTCT+AGG - Chr8:76753808-76753827 None:intergenic 30.0%
TTTGTAATCCAATATCCTCA+TGG - Chr8:76754682-76754701 None:intergenic 30.0%
! ATTTTGTGCTCTTCTTTCAA+TGG - Chr8:76753994-76754013 None:intergenic 30.0%
! GTTGTTGAATGATTTGTAAG+AGG + Chr8:76755307-76755326 MsG0880046685.01.T01:three_prime_UTR 30.0%
! TTGAATGATTTGTAAGAGGT+TGG + Chr8:76755311-76755330 MsG0880046685.01.T01:three_prime_UTR 30.0%
!! GCACAAAATATTTTTGTGGA+AGG + Chr8:76754005-76754024 MsG0880046685.01.T01:CDS 30.0%
!! GTTGGTTCTAATTTACATAG+TGG + Chr8:76755329-76755348 MsG0880046685.01.T01:three_prime_UTR 30.0%
!! TGATTTTGTTTCATCTGGTT+TGG + Chr8:76753513-76753532 MsG0880046685.01.T01:CDS 30.0%
!!! AGGTGATGTTTTGTTTTGTT+TGG + Chr8:76755496-76755515 MsG0880046685.01.T01:three_prime_UTR 30.0%
!!! ATTTTTGGGAGTGTTATTAG+TGG + Chr8:76753958-76753977 MsG0880046685.01.T01:CDS 30.0%
!!! CTTGTTGATTTTGTTTCATC+TGG + Chr8:76753508-76753527 MsG0880046685.01.T01:CDS 30.0%
!!! TGATGTTTTGTTTTGTTTGG+AGG + Chr8:76755499-76755518 MsG0880046685.01.T01:three_prime_UTR 30.0%
AACTGAACAAAAGGTGAAAC+AGG - Chr8:76755631-76755650 None:intergenic 35.0%
ACAGATACTAATGTACAAGG+AGG - Chr8:76754382-76754401 None:intergenic 35.0%
ACAGTTCTGCATAGAATCTA+TGG + Chr8:76753829-76753848 MsG0880046685.01.T01:CDS 35.0%
ACTGGTTACTATATGCTATG+TGG + Chr8:76755723-76755742 MsG0880046685.01.T01:three_prime_UTR 35.0%
ATGGGAAATTCATGGTTCAT+AGG + Chr8:76753848-76753867 MsG0880046685.01.T01:CDS 35.0%
CAATGACACTCTTGCATATT+AGG - Chr8:76755684-76755703 None:intergenic 35.0%
CAGTTCTGCATAGAATCTAT+GGG + Chr8:76753830-76753849 MsG0880046685.01.T01:CDS 35.0%
CATCTTTAAACGATAGAGAG+GGG - Chr8:76753363-76753382 None:intergenic 35.0%
CTGTTAGCAATATCATCTAC+CGG + Chr8:76753887-76753906 MsG0880046685.01.T01:CDS 35.0%
GTAGATCATAAACAACTTGC+AGG - Chr8:76753681-76753700 None:intergenic 35.0%
TACATAACTCACAGAATCAC+TGG + Chr8:76755015-76755034 MsG0880046685.01.T01:CDS 35.0%
TAGGGTCTTCAGAATCAAAA+AGG - Chr8:76753789-76753808 None:intergenic 35.0%
TGGGAAAACCCTAAACAAAT+TGG - Chr8:76753587-76753606 None:intergenic 35.0%
TGTTCTGTACAGTTCTTATC+AGG - Chr8:76753460-76753479 None:intergenic 35.0%
TTAAAAGAGCAACTAGAACG+AGG - Chr8:76754326-76754345 None:intergenic 35.0%
TTGCACAAACCCAAATCTAT+TGG + Chr8:76754039-76754058 MsG0880046685.01.T01:CDS 35.0%
! AAACAAAAGCATGATCTACG+TGG - Chr8:76753753-76753772 None:intergenic 35.0%
! AACAAAAGCATGATCTACGT+GGG - Chr8:76753752-76753771 None:intergenic 35.0%
! ATAGATTTGGGTTTGTGCAA+AGG - Chr8:76754039-76754058 None:intergenic 35.0%
! ATATTAGGAGGGAAAAGTGT+GGG - Chr8:76755669-76755688 None:intergenic 35.0%
! ATTGGTTAGCTCAGTGATAA+AGG + Chr8:76754119-76754138 MsG0880046685.01.T01:CDS 35.0%
! GGTTTTGCGTTATAAGATTC+AGG - Chr8:76754959-76754978 None:intergenic 35.0%
! GTGTGCAACCAATTTGTTTA+GGG + Chr8:76753576-76753595 MsG0880046685.01.T01:CDS 35.0%
! GTTCAGTTTTTGTTACCTCA+TGG + Chr8:76755643-76755662 MsG0880046685.01.T01:three_prime_UTR 35.0%
! TAATGGAATTGCTGAGCTTT+TGG + Chr8:76753933-76753952 MsG0880046685.01.T01:CDS 35.0%
! TCATGAACATTAGCTCCTTT+TGG - Chr8:76754176-76754195 None:intergenic 35.0%
! TCTTTCTTATGTTCTGAGTC+AGG - Chr8:76755058-76755077 None:intergenic 35.0%
! TGGTTCATAGGCCTTTTATA+AGG + Chr8:76753860-76753879 MsG0880046685.01.T01:CDS 35.0%
! TGTGTGCAACCAATTTGTTT+AGG + Chr8:76753575-76753594 MsG0880046685.01.T01:CDS 35.0%
! TTGGTTAGCTCAGTGATAAA+GGG + Chr8:76754120-76754139 MsG0880046685.01.T01:CDS 35.0%
! TTTTCTTGTGATTGTTCAGG+TGG + Chr8:76754896-76754915 MsG0880046685.01.T01:intron 35.0%
!! CTGAGCTTTTGGAGATTTTT+GGG + Chr8:76753944-76753963 MsG0880046685.01.T01:CDS 35.0%
!! GCTGTTTTCATAAGTTATCC+TGG - Chr8:76754525-76754544 None:intergenic 35.0%
!! GGCAATAGTACTACTAGTTT+TGG + Chr8:76753220-76753239 MsG0880046685.01.T01:CDS 35.0%
!! TCCTTTTGGCTATTAGTAAC+TGG - Chr8:76754162-76754181 None:intergenic 35.0%
!! TCGGATTTTGGTAACTTCTT+AGG - Chr8:76753172-76753191 None:intergenic 35.0%
!! TTTTGTTTGGAGGATCCATT+AGG + Chr8:76755509-76755528 MsG0880046685.01.T01:three_prime_UTR 35.0%
!!! TTGTGTTGTTTTTGCTTAGG+TGG + Chr8:76755465-76755484 MsG0880046685.01.T01:three_prime_UTR 35.0%
AATCAAGTGATGTAGCTGAG+AGG + Chr8:76755144-76755163 MsG0880046685.01.T01:CDS 40.0%
AGTCTACAAGCTCAAGTTTG+AGG - Chr8:76754742-76754761 None:intergenic 40.0%
CAACAAGGTCTAACAACGTT+TGG - Chr8:76753495-76753514 None:intergenic 40.0%
CACTGTAACCTGTTTCCTAA+TGG - Chr8:76755527-76755546 None:intergenic 40.0%
CATATTAGGAGGGAAAAGTG+TGG - Chr8:76755670-76755689 None:intergenic 40.0%
CATTAGGAAACAGGTTACAG+TGG + Chr8:76755525-76755544 MsG0880046685.01.T01:three_prime_UTR 40.0%
CTAATTTCACTAAGCTCTCC+AGG + Chr8:76754504-76754523 MsG0880046685.01.T01:intron 40.0%
CTTTGCAGAATCTGATGAGT+CGG - Chr8:76753191-76753210 None:intergenic 40.0%
GACACTCTTGCATATTAGGA+GGG - Chr8:76755680-76755699 None:intergenic 40.0%
GATTCTGCAAAGAGTGATTC+TGG + Chr8:76753199-76753218 MsG0880046685.01.T01:CDS 40.0%
GCCAGTTACTAATAGCCAAA+AGG + Chr8:76754158-76754177 MsG0880046685.01.T01:CDS 40.0%
GCTAATGTTCATGAGTGAGT+TGG + Chr8:76754182-76754201 MsG0880046685.01.T01:CDS 40.0%
GCTCGGTTTCAAAAACAAAC+CGG - Chr8:76753909-76753928 None:intergenic 40.0%
TACTCGAACGAAACTTAGGT+GGG - Chr8:76753606-76753625 None:intergenic 40.0%
TGACACTCTTGCATATTAGG+AGG - Chr8:76755681-76755700 None:intergenic 40.0%
TGAGAGGCGAAGAATAACAT+GGG + Chr8:76755160-76755179 MsG0880046685.01.T01:CDS 40.0%
TTGGCTAAGAGAAAACAACC+TGG - Chr8:76754302-76754321 None:intergenic 40.0%
! GAATCTGATGAGTCGGATTT+TGG - Chr8:76753184-76753203 None:intergenic 40.0%
! TTATGGTGCCTTTGTTTCGA+CGG + Chr8:76754247-76754266 MsG0880046685.01.T01:intron 40.0%
! TTATGTTCTGAGTCAGGTTC+AGG - Chr8:76755052-76755071 None:intergenic 40.0%
!! GCTGAGCTTTTGGAGATTTT+TGG + Chr8:76753943-76753962 MsG0880046685.01.T01:CDS 40.0%
!! TGAAGATAGCACTGGCAATA+GGG + Chr8:76755374-76755393 MsG0880046685.01.T01:three_prime_UTR 40.0%
!! TTGAAGATAGCACTGGCAAT+AGG + Chr8:76755373-76755392 MsG0880046685.01.T01:three_prime_UTR 40.0%
!!! GGTTTGTTTTTGAAACCGAG+CGG + Chr8:76753908-76753927 MsG0880046685.01.T01:CDS 40.0%
AAACAGGTTACAGTGGTGGT+GGG + Chr8:76755532-76755551 MsG0880046685.01.T01:three_prime_UTR 45.0%
AAGTTTCGTTCGAGTAGTGG+TGG + Chr8:76753610-76753629 MsG0880046685.01.T01:CDS 45.0%
AGAGCAACTAGAACGAGGAT+TGG - Chr8:76754321-76754340 None:intergenic 45.0%
AGCAATTCCATTATGCCGCT+CGG - Chr8:76753926-76753945 None:intergenic 45.0%
ATCCAAACTGGACATCCATG+AGG + Chr8:76754664-76754683 MsG0880046685.01.T01:intron 45.0%
ATCCTCATGGATGTCCAGTT+TGG - Chr8:76754669-76754688 None:intergenic 45.0%
CCACTACTCGAACGAAACTT+AGG - Chr8:76753610-76753629 None:intergenic 45.0%
CCTAAGTTTCGTTCGAGTAG+TGG + Chr8:76753607-76753626 MsG0880046685.01.T01:CDS 45.0%
CTACTCGAACGAAACTTAGG+TGG - Chr8:76753607-76753626 None:intergenic 45.0%
CTGAGAGGCGAAGAATAACA+TGG + Chr8:76755159-76755178 MsG0880046685.01.T01:CDS 45.0%
CTGGACATCCATGAGGATAT+TGG + Chr8:76754671-76754690 MsG0880046685.01.T01:intron 45.0%
GATGCTATCTCAAATGGACG+AGG + Chr8:76755076-76755095 MsG0880046685.01.T01:CDS 45.0%
GGCTGAATTCCAGAAGGTTA+TGG + Chr8:76754230-76754249 MsG0880046685.01.T01:intron 45.0%
GTGCTCTTCTTTCAATGGCA+AGG - Chr8:76753989-76754008 None:intergenic 45.0%
TAGCATGGCTGAATTCCAGA+AGG + Chr8:76754224-76754243 MsG0880046685.01.T01:CDS 45.0%
TAGGAAACAGGTTACAGTGG+TGG + Chr8:76755528-76755547 MsG0880046685.01.T01:three_prime_UTR 45.0%
TCTTGTGATTGTTCAGGTGG+CGG + Chr8:76754899-76754918 MsG0880046685.01.T01:intron 45.0%
TGGAGGATCCATTAGGAAAC+AGG + Chr8:76755516-76755535 MsG0880046685.01.T01:three_prime_UTR 45.0%
TTTCCTGCAAGCATGAATGC+TGG + Chr8:76753322-76753341 MsG0880046685.01.T01:CDS 45.0%
! CGGAACGAGCTCATTTGTTA+TGG + Chr8:76754919-76754938 MsG0880046685.01.T01:CDS 45.0%
! GTTCATGAGTGAGTTGGAAG+AGG + Chr8:76754188-76754207 MsG0880046685.01.T01:CDS 45.0%
! TAGCAGTAGCTGGTTTCACT+TGG - Chr8:76755247-76755266 None:intergenic 45.0%
! TTTGAAACCGAGCGGCATAA+TGG + Chr8:76753916-76753935 MsG0880046685.01.T01:CDS 45.0%
!! ACAAAGGCACCATAACCTTC+TGG - Chr8:76754242-76754261 None:intergenic 45.0%
!! GAGTGAGTTGGAAGAGGTTT+TGG + Chr8:76754194-76754213 MsG0880046685.01.T01:CDS 45.0%
!!! GGTTTTGGAGATGACTAGCA+TGG + Chr8:76754209-76754228 MsG0880046685.01.T01:CDS 45.0%
ACACCAGCATTCATGCTTGC+AGG - Chr8:76753328-76753347 None:intergenic 50.0%
ACCAGCTACTGCTACATGCT+CGG + Chr8:76755253-76755272 MsG0880046685.01.T01:CDS 50.0%
AGCCTGTGTTTGATCCTGCT+TGG + Chr8:76753650-76753669 MsG0880046685.01.T01:CDS 50.0%
ATGCCAACGCAAGATCGAAG+AGG + Chr8:76755112-76755131 MsG0880046685.01.T01:CDS 50.0%
CAAGCCAACCGTCGAAACAA+AGG - Chr8:76754258-76754277 None:intergenic 50.0%
CGAGAGGCAGAATCACTTGT+CGG - Chr8:76754980-76754999 None:intergenic 50.0%
CTTCCTCTTCGATCTTGCGT+TGG - Chr8:76755118-76755137 None:intergenic 50.0%
GAAACAGGTTACAGTGGTGG+TGG + Chr8:76755531-76755550 MsG0880046685.01.T01:three_prime_UTR 50.0%
GACCAAGCAGGATCAAACAC+AGG - Chr8:76753655-76753674 None:intergenic 50.0%
TTACACAACGCAGCGATTGC+TGG - Chr8:76753553-76753572 None:intergenic 50.0%
! AACAACCTGGTAATGCGAGC+TGG - Chr8:76754289-76754308 None:intergenic 50.0%
! AACAGGTTACAGTGGTGGTG+GGG + Chr8:76755533-76755552 MsG0880046685.01.T01:three_prime_UTR 50.0%
! TTGCTTAGGTGGTGCTGAGT+AGG + Chr8:76755476-76755495 MsG0880046685.01.T01:three_prime_UTR 50.0%
!! ACAAGTGCTGAGAACACGAG+AGG - Chr8:76754996-76755015 None:intergenic 50.0%
!!! TGGGGGGGTGTTTTATGTGT+CGG + Chr8:76755551-76755570 MsG0880046685.01.T01:three_prime_UTR 50.0%
ACCAGCGTCCAATCTGTAGG+CGG + Chr8:76755203-76755222 MsG0880046685.01.T01:CDS 55.0%
ACCGAGCATGTAGCAGTAGC+TGG - Chr8:76755257-76755276 None:intergenic 55.0%
ACTTGCAGGTGAGACCAAGC+AGG - Chr8:76753667-76753686 None:intergenic 55.0%
CCTTACCAGCTCGCATTACC+AGG + Chr8:76754281-76754300 MsG0880046685.01.T01:intron 55.0%
TACAGATTGGACGCTGGTGG+CGG - Chr8:76755201-76755220 None:intergenic 55.0%
! AGGGAAAAGTGTGGGCCATG+AGG - Chr8:76755661-76755680 None:intergenic 55.0%
! CCTGGTAATGCGAGCTGGTA+AGG - Chr8:76754284-76754303 None:intergenic 55.0%
! GGTGCCTTTGTTTCGACGGT+TGG + Chr8:76754251-76754270 MsG0880046685.01.T01:intron 55.0%
!! ACAGGTTACAGTGGTGGTGG+GGG + Chr8:76755534-76755553 MsG0880046685.01.T01:three_prime_UTR 55.0%
!!! GGGGGTGTTTTATGTGTCGG+TGG + Chr8:76755554-76755573 MsG0880046685.01.T01:three_prime_UTR 55.0%
ACCGCCTACAGATTGGACGC+TGG - Chr8:76755207-76755226 None:intergenic 60.0%
AGCGTCCAATCTGTAGGCGG+TGG + Chr8:76755206-76755225 MsG0880046685.01.T01:CDS 60.0%
GCCACCAGCGTCCAATCTGT+AGG + Chr8:76755200-76755219 MsG0880046685.01.T01:CDS 60.0%
GCCTACAGATTGGACGCTGG+TGG - Chr8:76755204-76755223 None:intergenic 60.0%
GTCCAATCTGTAGGCGGTGG+CGG + Chr8:76755209-76755228 MsG0880046685.01.T01:CDS 60.0%
!! AGGTTACAGTGGTGGTGGGG+GGG + Chr8:76755536-76755555 MsG0880046685.01.T01:three_prime_UTR 60.0%
!! CAGGTTACAGTGGTGGTGGG+GGG + Chr8:76755535-76755554 MsG0880046685.01.T01:three_prime_UTR 60.0%
CACCGCCACCGCCTACAGAT+TGG - Chr8:76755214-76755233 None:intergenic 65.0%
!! CGCTGGTGGCGGCTTCTTCA+AGG - Chr8:76755190-76755209 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 76753142 76755797 76753142 ID=MsG0880046685.01;Name=MsG0880046685.01
Chr8 mRNA 76753142 76755797 76753142 ID=MsG0880046685.01.T01;Parent=MsG0880046685.01;Name=MsG0880046685.01.T01;_AED=0.35;_eAED=0.35;_QI=0|0|0.5|1|1|1|2|511|491
Chr8 exon 76753142 76754245 76753142 ID=MsG0880046685.01.T01:exon:8594;Parent=MsG0880046685.01.T01
Chr8 exon 76754915 76755797 76754915 ID=MsG0880046685.01.T01:exon:8595;Parent=MsG0880046685.01.T01
Chr8 CDS 76753142 76754245 76753142 ID=MsG0880046685.01.T01:cds;Parent=MsG0880046685.01.T01
Chr8 CDS 76754915 76755286 76754915 ID=MsG0880046685.01.T01:cds;Parent=MsG0880046685.01.T01
Chr8 three_prime_UTR 76755287 76755797 76755287 ID=MsG0880046685.01.T01:three_prime_utr;Parent=MsG0880046685.01.T01
Gene Sequence

>MsG0880046685.01.T01

ATGATAAAGCAAATTCTTAGCAAATTGCCTAAGAAGTTACCAAAATCCGACTCATCAGATTCTGCAAAGAGTGATTCTGGCAATAGTACTACTAGTTTTGGAAATGTATTTCAGTGTACAAATGTTGGAAGTACCATTTCAAGCAAATTAAATGTTGTGAAAAGGGTATCTTCTGTTGTTTTTCCTGCAAGCATGAATGCTGGTGTAGAAGCAGTTGACCCCTCTCTATCGTTTAAAGATGTTTCAAATACACAGAAACAAAGCCTTTTTATCAGTAAGTTGAACCTTTGCTGCAAAGTTTATGATATGAGTGATCCTGATAAGAACTGTACAGAACAAGATGTTAAACGCCAAACGTTGTTAGACCTTGTTGATTTTGTTTCATCTGGTTTGGTAAAATTTACAGAACCAGCAATCGCTGCGTTGTGTAAAATGTGTGCAACCAATTTGTTTAGGGTTTTCCCACCTAAGTTTCGTTCGAGTAGTGGTGGAGAAACAGAAGATGAAGAGCCTGTGTTTGATCCTGCTTGGTCTCACCTGCAAGTTGTTTATGATCTACTGCTTCAATTCATCAATTACAACTCTCTTGATGTGAAGCTTGCAAAATCCCACGTAGATCATGCTTTTGTTTTAAGATTACTTGACCTTTTTGATTCTGAAGACCCTAGAGAAAGAGATTGTTTGAAAACAGTTCTGCATAGAATCTATGGGAAATTCATGGTTCATAGGCCTTTTATAAGGAAATCTGTTAGCAATATCATCTACCGGTTTGTTTTTGAAACCGAGCGGCATAATGGAATTGCTGAGCTTTTGGAGATTTTTGGGAGTGTTATTAGTGGATTTGCCTTGCCATTGAAAGAAGAGCACAAAATATTTTTGTGGAAGGCTTTAATTCCTTTGCACAAACCCAAATCTATTGGTATTTATCATCAGCAGTTAACATATTGCATTGTACAGTTCATAGATAAGGATCAAAGATTGGTTAGCTCAGTGATAAAGGGACTATTGAAGTATTGGCCAGTTACTAATAGCCAAAAGGAGCTAATGTTCATGAGTGAGTTGGAAGAGGTTTTGGAGATGACTAGCATGGCTGAATTCCAGAAGGTGGCGGAACGAGCTCATTTGTTATGGAACAATGAGCACATCCTGAATCTTATAACGCAAAACCGACAAGTGATTCTGCCTCTCGTGTTCTCAGCACTTGTACATAACTCACAGAATCACTGGAATCAAGCAGTCCTGAACCTGACTCAGAACATAAGAAAGATGCTATCTCAAATGGACGAGGAACTCGTACTTGCATGCCAACGCAAGATCGAAGAGGAAGATTCAAAATCAAGTGATGTAGCTGAGAGGCGAAGAATAACATGGGAACGCCTTGAAGAAGCCGCCACCAGCGTCCAATCTGTAGGCGGTGGCGGTGATATTTTAGTCCAAGTGAAACCAGCTACTGCTACATGCTCGGTAGCATGCTAA
Protein sequence

>MsG0880046685.01.T01

MIKQILSKLPKKLPKSDSSDSAKSDSGNSTTSFGNVFQCTNVGSTISSKLNVVKRVSSVVFPASMNAGVEAVDPSLSFKDVSNTQKQSLFISKLNLCCKVYDMSDPDKNCTEQDVKRQTLLDLVDFVSSGLVKFTEPAIAALCKMCATNLFRVFPPKFRSSSGGETEDEEPVFDPAWSHLQVVYDLLLQFINYNSLDVKLAKSHVDHAFVLRLLDLFDSEDPRERDCLKTVLHRIYGKFMVHRPFIRKSVSNIIYRFVFETERHNGIAELLEIFGSVISGFALPLKEEHKIFLWKALIPLHKPKSIGIYHQQLTYCIVQFIDKDQRLVSSVIKGLLKYWPVTNSQKELMFMSELEEVLEMTSMAEFQKVAERAHLLWNNEHILNLITQNRQVILPLVFSALVHNSQNHWNQAVLNLTQNIRKMLSQMDEELVLACQRKIEEEDSKSSDVAERRRITWERLEEAATSVQSVGGGGDILVQVKPATATCSVAC*