Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046772.01.T01 | XP_003609309.1 | 88.228 | 858 | 80 | 6 | 1 | 854 | 1 | 841 | 0 | 1515 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046772.01.T01 | A0A075F7E9 | 33.605 | 860 | 460 | 32 | 36 | 853 | 19 | 809 | 5.54E-123 | 393 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0480018450.01 | MsG0880046772.01 | 0.801128 | 1.042288e-48 | 4.938950e-46 |
| MsG0480021405.01 | MsG0880046772.01 | 0.802709 | 4.932305e-49 | 2.432882e-46 |
| MsG0480021499.01 | MsG0880046772.01 | 0.802476 | 5.509036e-49 | 2.701199e-46 |
| MsG0580028171.01 | MsG0880046772.01 | 0.815808 | 7.646789e-52 | 5.325887e-49 |
| MsG0680035171.01 | MsG0880046772.01 | 0.813107 | 3.025550e-51 | 1.957941e-48 |
| MsG0880044283.01 | MsG0880046772.01 | 0.851099 | 1.106259e-60 | 2.214503e-57 |
| MsG0880046769.01 | MsG0880046772.01 | 0.881012 | 3.416377e-70 | 1.968678e-66 |
| MsG0880046770.01 | MsG0880046772.01 | 0.864037 | 1.621818e-64 | 5.020610e-61 |
| MsG0880046771.01 | MsG0880046772.01 | 0.925099 | 2.901813e-90 | 1.270681e-85 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046772.01.T01 | MTR_4g114280 | 88.228 | 858 | 80 | 6 | 1 | 854 | 1 | 841 | 0.0 | 1515 |
| MsG0880046772.01.T01 | MTR_4g114240 | 73.318 | 862 | 187 | 11 | 1 | 854 | 1 | 827 | 0.0 | 1235 |
| MsG0880046772.01.T01 | MTR_4g114250 | 42.033 | 797 | 409 | 19 | 60 | 847 | 53 | 805 | 0.0 | 590 |
| MsG0880046772.01.T01 | MTR_4g114270 | 41.521 | 802 | 404 | 23 | 60 | 847 | 53 | 803 | 0.0 | 582 |
| MsG0880046772.01.T01 | MTR_5g013130 | 35.523 | 822 | 431 | 26 | 59 | 848 | 38 | 792 | 3.77e-138 | 430 |
| MsG0880046772.01.T01 | MTR_8g011370 | 35.132 | 834 | 452 | 28 | 45 | 847 | 29 | 804 | 5.61e-137 | 427 |
| MsG0880046772.01.T01 | MTR_5g013070 | 34.704 | 827 | 437 | 27 | 57 | 848 | 37 | 795 | 2.30e-131 | 412 |
| MsG0880046772.01.T01 | MTR_8g011410 | 34.371 | 835 | 451 | 29 | 45 | 843 | 29 | 802 | 3.37e-129 | 412 |
| MsG0880046772.01.T01 | MTR_2g067980 | 31.829 | 864 | 483 | 28 | 17 | 848 | 234 | 1023 | 1.49e-123 | 398 |
| MsG0880046772.01.T01 | MTR_5g006160 | 33.292 | 811 | 448 | 28 | 55 | 847 | 33 | 768 | 1.60e-123 | 391 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046772.01.T01 | AT5G24080 | 30.139 | 866 | 525 | 31 | 10 | 854 | 13 | 819 | 4.41e-98 | 326 |
| MsG0880046772.01.T01 | AT1G34300 | 29.878 | 820 | 476 | 29 | 45 | 846 | 27 | 765 | 2.05e-93 | 312 |
| MsG0880046772.01.T01 | AT5G24080 | 30.110 | 724 | 456 | 23 | 149 | 854 | 1 | 692 | 1.61e-85 | 289 |
| MsG0880046772.01.T01 | AT5G24080 | 30.110 | 724 | 456 | 23 | 149 | 854 | 1 | 692 | 1.61e-85 | 289 |
| MsG0880046772.01.T01 | AT2G19130 | 30.477 | 817 | 457 | 36 | 62 | 847 | 40 | 776 | 1.06e-82 | 283 |
| MsG0880046772.01.T01 | AT4G32300 | 28.501 | 814 | 475 | 28 | 62 | 852 | 50 | 779 | 3.95e-82 | 281 |
| MsG0880046772.01.T01 | AT4G00340 | 29.774 | 796 | 462 | 27 | 67 | 839 | 40 | 761 | 3.13e-79 | 273 |
| MsG0880046772.01.T01 | AT4G00340 | 29.167 | 792 | 451 | 28 | 67 | 839 | 40 | 740 | 2.25e-78 | 270 |
| MsG0880046772.01.T01 | AT5G35370 | 28.947 | 836 | 464 | 35 | 62 | 845 | 52 | 809 | 6.82e-75 | 262 |
| MsG0880046772.01.T01 | AT1G11340 | 28.010 | 839 | 471 | 32 | 66 | 850 | 40 | 799 | 2.43e-71 | 251 |
Find 188 sgRNAs with CRISPR-Local
Find 227 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACACAACCACTGTTGTT+TGG | 0.304063 | 8:-77924763 | MsG0880046772.01.T01:CDS |
| AAAACATTGATATACCCAGG+TGG | 0.788113 | 8:+77922687 | None:intergenic |
| AAACATTGACTATGATGATC+AGG | 0.436681 | 8:+77924200 | None:intergenic |
| AAAGCTGACGTCTATAGTTT+TGG | 0.272653 | 8:-77922790 | MsG0880046772.01.T01:CDS |
| AAATACTTATAGACGAAGCA+TGG | 0.577777 | 8:-77922942 | MsG0880046772.01.T01:CDS |
| AAATAGGGGATGGCACAAGC+AGG | 0.611649 | 8:-77922858 | MsG0880046772.01.T01:CDS |
| AACAGCAAAACCATTACCTT+TGG | 0.368456 | 8:+77924811 | None:intergenic |
| AACCCAACCCAATGATTCTG+GGG | 0.634546 | 8:+77924906 | None:intergenic |
| AACCTCGGGCTTAGGCACAT+TGG | 0.596631 | 8:+77923566 | None:intergenic |
| AACTTTAAGAATATGTCTTG+GGG | 0.599069 | 8:-77923819 | MsG0880046772.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAATACTTATAGACGAAGCA+TGG | - | Chr8:77924572-77924591 | MsG0880046772.01.T01:CDS | 30.0% | |
| AAGTAATCCTAAATTGCCTA+TGG | - | Chr8:77923604-77923623 | MsG0880046772.01.T01:CDS | 30.0% | |
| AATGATCCTATCTCTTACTT+TGG | - | Chr8:77923997-77924016 | MsG0880046772.01.T01:CDS | 30.0% | |
| ACACTAGCTGCAATAAATAT+AGG | - | Chr8:77923813-77923832 | MsG0880046772.01.T01:CDS | 30.0% | |
| AGAAAAAGAACAGTAACAAC+AGG | + | Chr8:77922506-77922525 | None:intergenic | 30.0% | |
| AGAGAAAATGAAACTTGCAT+TGG | - | Chr8:77924474-77924493 | MsG0880046772.01.T01:CDS | 30.0% | |
| AGGAATTTGGTTAAGCTAAT+TGG | - | Chr8:77924349-77924368 | MsG0880046772.01.T01:CDS | 30.0% | |
| ATCACAGGTATAACAGAATA+TGG | + | Chr8:77923728-77923747 | None:intergenic | 30.0% | |
| ATGATCCTATCTCTTACTTT+GGG | - | Chr8:77923998-77924017 | MsG0880046772.01.T01:CDS | 30.0% | |
| CAAATTCAACATGGATTTAG+AGG | + | Chr8:77922805-77922824 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 77922486 | 77925050 | 77922486 | ID=MsG0880046772.01;Name=MsG0880046772.01 |
| Chr8 | mRNA | 77922486 | 77925050 | 77922486 | ID=MsG0880046772.01.T01;Parent=MsG0880046772.01;Name=MsG0880046772.01.T01;_AED=0.28;_eAED=0.28;_QI=0|-1|0|1|-1|1|1|0|854 |
| Chr8 | exon | 77922486 | 77925050 | 77922486 | ID=MsG0880046772.01.T01:exon:18570;Parent=MsG0880046772.01.T01 |
| Chr8 | CDS | 77922486 | 77925050 | 77922486 | ID=MsG0880046772.01.T01:cds;Parent=MsG0880046772.01.T01 |
| Gene Sequence |
>MsG0880046772.01.T01 ATGGCTTACAGCCCTGTCCTGTTGTTACTGTTCTTTTTCTTCATGCTATTTACTTGTTCATGCCTTGACTTCTCACAAACGCGTACTGGGAAGCCTAATCCAGATGCAACAACTGAAAAACACCCCAGAATCATTGGGTTGGGTTCTTTGCTCTATCCTAAAGGCGAAAATACTTCATGGCCATCACGTTCTGGCCATTTTGCTTTTGGGTTTTACCCCAAAGGTAATGGTTTTGCTGTTGGAATATGGTTGGTTAATCCATCTGAAAACACAACCACTGTTGTTTGGACTGCTAACCGTAATGCTCCAGCAGTCTCCTCTAAATCCATGTTGAATTTGACAGAGCAAGGCCTGCTTCTTCAAAATGGAAATGGAGATTCACCTATTAATATAGGTTCGAGAGATGATTCAGATTCAGATTCAAAGTTGGTCTCTAATGCATCTATGCATGATTCTGGCAATTTTGTGCTTTATGATCATGATTTTAATGTTACATGGCAAAGCTTTGATCACCCAACTGACACCATATTAGGAGGTCAGAGTTTGACTGCTGGTGATTATTTAATCTCCAGTGTGTCCAAATCAGACCATTCTAGTGGACGTTTCTATCTGGAGGTGCAAAATGGTAGAAGCGTTGCTGCTTACCCTTTTTACAGCTTCAGAAGTGATGAGGATGCTTACTGGAATTCAAACACTTCGCATCAGATATACGTACAACAGCTCAGTCTTAACATTCAAGGCTTTCTTTGTGTAAATGGTTCAAGTGGCAGTGCTATAACTTGTAAAAATTTAAATCGACTTTACCCTTGTAGACGAATTTATGAATCTCCTGATCATCATAGTCAATGTTTCAACTATACAAATAAGCCTAGAAAGAAGTCAAACAACGCAACTTCAATCTACCGTGCAACACTTGACGTAGATGGAAACTTGAGATTGTATGAACACCAATTTCATTTTGAAGGCAACAATAGCTCTTGTGTGGTAGTGTTGTGGAAAGCACTTAATGAGACTTGTTTAGTGAAGGGGTTTTGTGGGTTGAACAGTTATTGCACCTCCAACATCAGCGGTGATGCTGTCTGCAAGTGTTATCCTGGTTTCATCCTTTCCGAGACCAAAAGTAATCCTAAATTGCCTATGGACTGCGTACAGAAACATAGCAAATATGACTGTGAAAGCAGTGAAGGCACGGCGTTATATAATTATACTAACTTTAAGAATATGTCTTGGGGTGATATTCCATATTCTGTTATACCTGTGATGAACATGAAAACTTGTGAGCAGGCTTGCCAAGAAGATTGTGTTTGCGGGGGAGCAATATATACAAACACTAGCTGCAATAAATATAGGCTTCCAATTATTTATGGCAGGGTTCAAAATTATTCTTCCACCGTGTCTGTGGCCCTATTAAAGATTCGTTCTTCAACAACTGCCATTATTTCACCTCCAACCACAAACAGCACCAATGTGCCTAAGCCCGAGGTTGTTGTTGACAGTAAGAGGAATCTGATAATGATCCTATCTCTTACTTTGGGCGTTGTTGCATTAATTTGTTTGGTCTTTGCAGTGTCCGTTTTCTTCACTTATAGGCGTCAAGTTAATAGGTACGCAATGTTGTCAGAAAGTGAAAAGCTTGAATTTACTGAAGAATGTTCCCTGCGTTCATTTTCTTTTGACGAACTTGCAAAATCAACTGGGGGATTTTCAGAAGAGATAGGGAGAGGATCATTTGGAGTTGTTTATAAAGGTAAATTGGGCAACAATAACAAAAGTATTGCCGTGAAGAGACTGGAGGAGAGAATTACTGATGCAGGGGAGAGGGAGTTCCAAGCTGAAATTACTGCCATTGCTCGAACTCATCATAGGAATTTGGTTAAGCTAATTGGTTTTTGTATAGAAGGATCCAAGAAGCTCCTTGTTTATGAATTTGTCAGCAAAGGATCTCTTGCAAATCTCCTCTTTGAAGGGGAAACGAGACTATCTTGGAAAGAGAAAATGAAACTTGCATTGGATGTGGCTAGAGGTCTTTTGTATCTACACGAAGAGTGTGATGTCCGAATCATCCATTGCAATATAAATCCTCAAAAAATACTTATAGACGAAGCATGGACTGCAAAAATATCTGATTTTGGATTCGCAAGGTTATCAAAGAGGGGCCATTCAAGAACTAAAATAGGGGATGGCACAAGCAGGTACTTGGCACCCGAATGGCAGAAGGAGGATGCATCAGTATCAGTAAAAGCTGACGTCTATAGTTTTGGGGTGGTACTATTGGAGATTATTTGCCGCAAAAGAAGTATAGAGATGAATAATATTTCCTCGGCCGATGAAATCCCTCTTTCCACCTGGGTATATCAATGTTTTGCATCAGGACAATTGAACAAGCTAATCACACATAATGAAAATGATATGGATTGGAAGATATTGGAAAGAATGGTGAAGGTGGGGTTGTGGTGCGTGCAAGACCACCAATCTCTCCGTCCTGGAATGAAAAATGTAATCTTGATGTTAGAAGGTTTGAAAGATATTCCAGTTCCTCCCTCTGCAGCTCGCTTGCTTGAGTAG |
| Protein sequence |
>MsG0880046772.01.T01 MAYSPVLLLLFFFFMLFTCSCLDFSQTRTGKPNPDATTEKHPRIIGLGSLLYPKGENTSWPSRSGHFAFGFYPKGNGFAVGIWLVNPSENTTTVVWTANRNAPAVSSKSMLNLTEQGLLLQNGNGDSPINIGSRDDSDSDSKLVSNASMHDSGNFVLYDHDFNVTWQSFDHPTDTILGGQSLTAGDYLISSVSKSDHSSGRFYLEVQNGRSVAAYPFYSFRSDEDAYWNSNTSHQIYVQQLSLNIQGFLCVNGSSGSAITCKNLNRLYPCRRIYESPDHHSQCFNYTNKPRKKSNNATSIYRATLDVDGNLRLYEHQFHFEGNNSSCVVVLWKALNETCLVKGFCGLNSYCTSNISGDAVCKCYPGFILSETKSNPKLPMDCVQKHSKYDCESSEGTALYNYTNFKNMSWGDIPYSVIPVMNMKTCEQACQEDCVCGGAIYTNTSCNKYRLPIIYGRVQNYSSTVSVALLKIRSSTTAIISPPTTNSTNVPKPEVVVDSKRNLIMILSLTLGVVALICLVFAVSVFFTYRRQVNRYAMLSESEKLEFTEECSLRSFSFDELAKSTGGFSEEIGRGSFGVVYKGKLGNNNKSIAVKRLEERITDAGEREFQAEITAIARTHHRNLVKLIGFCIEGSKKLLVYEFVSKGSLANLLFEGETRLSWKEKMKLALDVARGLLYLHEECDVRIIHCNINPQKILIDEAWTAKISDFGFARLSKRGHSRTKIGDGTSRYLAPEWQKEDASVSVKADVYSFGVVLLEIICRKRSIEMNNISSADEIPLSTWVYQCFASGQLNKLITHNENDMDWKILERMVKVGLWCVQDHQSLRPGMKNVILMLEGLKDIPVPPSAARLLE* |
