AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580028171.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580028171.01.T01 MTR_5g088980 88.095 42 5 0 28 69 178 219 8.47e-20 87.8
MsG0580028171.01.T01 MTR_5g029260 71.739 46 13 0 195 240 69 114 4.96e-12 64.7
MsG0580028171.01.T01 MTR_7g089080 64.286 42 15 0 28 69 212 253 3.13e-11 63.5
MsG0580028171.01.T01 MTR_1g067150 78.788 33 7 0 69 101 79 111 5.00e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580028171.01.T01 AT2G26760 71.053 38 11 0 32 69 147 184 2.01e-12 66.2

Find 46 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATGTAAATGCTAACTTTA+AGG 0.110035 5:-76121179 MsG0580028171.01.T01:CDS
TTCTACAATGGCAGGGTATT+GGG 0.296126 5:-76121223 MsG0580028171.01.T01:CDS
CTCATCTTAGCATTTATATC+TGG 0.296448 5:+76121307 None:intergenic
GAAGTATCAGCTTTCTTTGC+CGG 0.302508 5:+76120947 None:intergenic
TTTCTACAATGGCAGGGTAT+TGG 0.338064 5:-76121224 MsG0580028171.01.T01:CDS
GCACTATCAGCTTTCTTTGC+GGG 0.352953 5:+76120727 None:intergenic
TATGTAAATGCTAACTTTAA+GGG 0.364959 5:-76121178 MsG0580028171.01.T01:CDS
AGAAGCCATACTGCTGCCTT+TGG 0.370792 5:+76121570 None:intergenic
TTGGGAGGCAATGGGTTTGT+TGG 0.385926 5:-76120039 MsG0580028171.01.T01:CDS
TGATATGTAGATTGTAGTCT+TGG 0.387607 5:-76120058 MsG0580028171.01.T01:intron
TCATTTGAAGCTTTCTTTGT+GGG 0.390405 5:+76120497 None:intergenic
TAGAAATCATAAGCTTAAAC+AGG 0.401284 5:-76119981 MsG0580028171.01.T01:CDS
TTCATTTGAAGCTTTCTTTG+TGG 0.401537 5:+76120496 None:intergenic
GTTCTACTTACTCAGATTCA+TGG 0.416229 5:-76119854 MsG0580028171.01.T01:CDS
GCTTATGATTTCTATGCCCT+TGG 0.419174 5:+76119990 None:intergenic
CGGAAAATTTCTACAATGGC+AGG 0.420430 5:-76121231 MsG0580028171.01.T01:CDS
GGCTCAGAAACTTGCAGCAA+AGG 0.449435 5:-76120540 MsG0580028171.01.T01:intron
ATTGTAGTCTTGGGAGGCAA+TGG 0.455853 5:-76120048 MsG0580028171.01.T01:CDS
CTCTTCCAAAGGCAGCAGTA+TGG 0.458219 5:-76121575 MsG0580028171.01.T01:CDS
AAAGGCAGCAGTATGGCTTC+TGG 0.465155 5:-76121568 MsG0580028171.01.T01:CDS
GATGGAACAACTTTAGCTGC+AGG 0.471970 5:+76120986 None:intergenic
ATGAGATCAATTCTTATTGA+CGG 0.472397 5:-76121289 MsG0580028171.01.T01:CDS
TTACTTACATTGGGAAACAT+AGG 0.475384 5:+76121151 None:intergenic
TGAGATCAATTCTTATTGAC+GGG 0.486231 5:-76121288 MsG0580028171.01.T01:CDS
TAGGAAATTTGAACTTATGC+CGG 0.494203 5:-76121251 MsG0580028171.01.T01:CDS
GATATGTAGATTGTAGTCTT+GGG 0.505411 5:-76120057 MsG0580028171.01.T01:intron
TTGTAGTCTTGGGAGGCAAT+GGG 0.505552 5:-76120047 MsG0580028171.01.T01:CDS
GAGCCATTGTATTGTTCTTG+AGG 0.531342 5:+76121595 None:intergenic
AGCACTATCAGCTTTCTTTG+CGG 0.535090 5:+76120726 None:intergenic
TGTTCGTGTGCATGATTACA+TGG 0.536076 5:-76121338 MsG0580028171.01.T01:CDS
CTCAGATTCATGGATAGTGT+TGG 0.544028 5:-76119844 MsG0580028171.01.T01:CDS
TGTTGGTTCTGCTATATGCA+AGG 0.547176 5:-76120022 MsG0580028171.01.T01:CDS
ATCTTCTAACGAAGAAGATA+AGG 0.547986 5:-76120674 MsG0580028171.01.T01:intron
ATGCAAGGCAGCAGTATCCA+AGG 0.548782 5:-76120007 MsG0580028171.01.T01:CDS
TGATTTACAACTAAATGAAG+TGG 0.551226 5:+76121532 None:intergenic
CAATACAATGGCTCTTCCAA+AGG 0.576376 5:-76121586 MsG0580028171.01.T01:CDS
ATGCCGGAAAATTTCTACAA+TGG 0.584945 5:-76121235 MsG0580028171.01.T01:CDS
GGATAGTGTTGGTACTACAT+AGG 0.596128 5:-76119833 None:intergenic
ACGGGTTGATCGAAGTGCAT+AGG 0.604673 5:-76121270 MsG0580028171.01.T01:CDS
AGATTCATCAGAGTCGTCAT+CGG 0.622764 5:+76120693 None:intergenic
ATGTAGATTGTAGTCTTGGG+AGG 0.624281 5:-76120054 MsG0580028171.01.T01:intron
GGAAAATTTCTACAATGGCA+GGG 0.630591 5:-76121230 MsG0580028171.01.T01:CDS
TGAATCTTCTGATGAAGATG+AGG 0.644258 5:-76120909 MsG0580028171.01.T01:CDS
ACTAAATGAAGTGGACTCTG+TGG 0.651058 5:+76121541 None:intergenic
TGCAAGGCAGCAGTATCCAA+GGG 0.655279 5:-76120006 MsG0580028171.01.T01:CDS
ATGCCTCAAGAACAATACAA+TGG 0.701209 5:-76121598 MsG0580028171.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATACAATGATATAAAATTA+AGG + Chr5:76120587-76120606 None:intergenic 10.0%
!! ATACAATGATATAAAATTAA+GGG + Chr5:76120586-76120605 None:intergenic 10.0%
!!! CTTTATTTTGTTTTTTAAAA+TGG - Chr5:76120844-76120863 MsG0580028171.01.T01:intron 10.0%
!!! TAAATATATATTTTTTCCTT+TGG - Chr5:76120871-76120890 MsG0580028171.01.T01:intron 10.0%
!!! TTGTTTTAATTTTTTTTTCA+TGG - Chr5:76120636-76120655 MsG0580028171.01.T01:intron 10.0%
!! AATAAACAAAACAAAAAGTT+GGG + Chr5:76120380-76120399 None:intergenic 15.0%
!! TAATAAACAAAACAAAAAGT+TGG + Chr5:76120381-76120400 None:intergenic 15.0%
!! TATATAGTGAATTAATTGAT+AGG - Chr5:76121237-76121256 MsG0580028171.01.T01:CDS 15.0%
!!! ATGTTTTAGCTATAAATTAA+AGG + Chr5:76120351-76120370 None:intergenic 15.0%
!! AGAATAAAAGGATAATGAAT+TGG - Chr5:76119924-76119943 MsG0580028171.01.T01:intron 20.0%
!! ATATGTAAATGCTAACTTTA+AGG - Chr5:76120253-76120272 MsG0580028171.01.T01:intron 20.0%
!! ATGTAATCAAATCATATGAA+AGG + Chr5:76120987-76121006 None:intergenic 20.0%
!! CAATTCTAATTACTTACATT+GGG + Chr5:76120293-76120312 None:intergenic 20.0%
!! GTTGTAAATCATAGAATAAA+AGG - Chr5:76119912-76119931 MsG0580028171.01.T01:intron 20.0%
!! TATGTAAATGCTAACTTTAA+GGG - Chr5:76120254-76120273 MsG0580028171.01.T01:intron 20.0%
!! TGTATTTACAACTATTTGTT+TGG - Chr5:76120601-76120620 MsG0580028171.01.T01:intron 20.0%
!! TTTAAATATTACTGTTCTTG+TGG + Chr5:76121013-76121032 None:intergenic 20.0%
! AAAGTTGTTCCATCTAAAAA+TGG - Chr5:76120455-76120474 MsG0580028171.01.T01:intron 25.0%
! AAGTTGTTCCATCTAAAAAT+GGG - Chr5:76120456-76120475 MsG0580028171.01.T01:intron 25.0%
! ATGAGATCAATTCTTATTGA+CGG - Chr5:76120143-76120162 MsG0580028171.01.T01:intron 25.0%
! CAAATACACAAAAACAAAAG+AGG + Chr5:76121530-76121549 None:intergenic 25.0%
! GCAATTCTAATTACTTACAT+TGG + Chr5:76120294-76120313 None:intergenic 25.0%
! GTGTTACAATTTAGTTAGTA+AGG + Chr5:76121340-76121359 None:intergenic 25.0%
! TAGAAATCATAAGCTTAAAC+AGG - Chr5:76121451-76121470 MsG0580028171.01.T01:intron 25.0%
! TGATTTACAACTAAATGAAG+TGG + Chr5:76119903-76119922 None:intergenic 25.0%
!!! AATTAAAGGTATCATGATGA+TGG + Chr5:76120337-76120356 None:intergenic 25.0%
!!! ACTCACTTACATGTTTTTTA+TGG - Chr5:76120415-76120434 MsG0580028171.01.T01:intron 25.0%
!!! ATGCTACCTTTCTTTTTTTT+TGG - Chr5:76121043-76121062 MsG0580028171.01.T01:intron 25.0%
AAAGTTGTTCCTTCCAAAAA+TGG - Chr5:76120676-76120695 MsG0580028171.01.T01:CDS 30.0%
AACATGTAAGTGAGTTATAG+AGG + Chr5:76120410-76120429 None:intergenic 30.0%
ACATGTAAGTGAGTTATAGA+GGG + Chr5:76120409-76120428 None:intergenic 30.0%
ATCTTCTAACGAAGAAGATA+AGG - Chr5:76120758-76120777 MsG0580028171.01.T01:CDS 30.0%
CATAAAATATGCAAGGTTCA+GGG + Chr5:76121087-76121106 None:intergenic 30.0%
CCACTACCAAAAAAAAAGAA+AGG + Chr5:76121052-76121071 None:intergenic 30.0%
CTCATCTTAGCATTTATATC+TGG + Chr5:76120128-76120147 None:intergenic 30.0%
GACAATGCATAAAATATGCA+AGG + Chr5:76121094-76121113 None:intergenic 30.0%
GATATGTAGATTGTAGTCTT+GGG - Chr5:76121375-76121394 MsG0580028171.01.T01:intron 30.0%
TAACGAAGAAGATAAGGTAT+TGG - Chr5:76120764-76120783 MsG0580028171.01.T01:intron 30.0%
TAGGAAATTTGAACTTATGC+CGG - Chr5:76120181-76120200 MsG0580028171.01.T01:intron 30.0%
TGAGATCAATTCTTATTGAC+GGG - Chr5:76120144-76120163 MsG0580028171.01.T01:intron 30.0%
TGATATGTAGATTGTAGTCT+TGG - Chr5:76121374-76121393 MsG0580028171.01.T01:intron 30.0%
TTACTTACATTGGGAAACAT+AGG + Chr5:76120284-76120303 None:intergenic 30.0%
! TCATTTGAAGCTTTCTTTGT+GGG + Chr5:76120938-76120957 None:intergenic 30.0%
! TTCATTTGAAGCTTTCTTTG+TGG + Chr5:76120939-76120958 None:intergenic 30.0%
!! TGACTGAGATTTTGTTTAAC+AGG - Chr5:76121548-76121567 MsG0580028171.01.T01:CDS 30.0%
!!! CCTTTCTTTTTTTTTGGTAG+TGG - Chr5:76121049-76121068 MsG0580028171.01.T01:intron 30.0%
ACACAAAAACAAAAGAGGTG+TGG + Chr5:76121525-76121544 None:intergenic 35.0%
ATGCCGGAAAATTTCTACAA+TGG - Chr5:76120197-76120216 MsG0580028171.01.T01:intron 35.0%
ATGCCTCAAGAACAATACAA+TGG - Chr5:76119834-76119853 MsG0580028171.01.T01:CDS 35.0%
CTTCTAACCTTCTAGTAGAA+AGG + Chr5:76121154-76121173 None:intergenic 35.0%
GCATAAAATATGCAAGGTTC+AGG + Chr5:76121088-76121107 None:intergenic 35.0%
GGAAAATTTCTACAATGGCA+GGG - Chr5:76120202-76120221 MsG0580028171.01.T01:intron 35.0%
GTTCTACTTACTCAGATTCA+TGG - Chr5:76121578-76121597 MsG0580028171.01.T01:CDS 35.0%
TATGCTACCTTTCTACTAGA+AGG - Chr5:76121144-76121163 MsG0580028171.01.T01:intron 35.0%
TGAATCTTCTGATGAAGATG+AGG - Chr5:76120523-76120542 MsG0580028171.01.T01:CDS 35.0%
! CTGCCATTGTAGAAATTTTC+CGG + Chr5:76120203-76120222 None:intergenic 35.0%
!! GAGATTTTGTTTAACAGGTC+AGG - Chr5:76121553-76121572 MsG0580028171.01.T01:CDS 35.0%
!!! TCTTTTTTTTTGGTAGTGGC+CGG - Chr5:76121053-76121072 MsG0580028171.01.T01:intron 35.0%
AGATTCATCAGAGTCGTCAT+CGG + Chr5:76120742-76120761 None:intergenic 40.0%
AGCACTATCAGCTTTCTTTG+CGG + Chr5:76120709-76120728 None:intergenic 40.0%
CAATACAATGGCTCTTCCAA+AGG - Chr5:76119846-76119865 MsG0580028171.01.T01:CDS 40.0%
CGGAAAATTTCTACAATGGC+AGG - Chr5:76120201-76120220 MsG0580028171.01.T01:intron 40.0%
CTCAGATTCATGGATAGTGT+TGG - Chr5:76121588-76121607 MsG0580028171.01.T01:CDS 40.0%
GAGCCATTGTATTGTTCTTG+AGG + Chr5:76119840-76119859 None:intergenic 40.0%
GCTTATGATTTCTATGCCCT+TGG + Chr5:76121445-76121464 None:intergenic 40.0%
TGTTCGTGTGCATGATTACA+TGG - Chr5:76120094-76120113 MsG0580028171.01.T01:intron 40.0%
TTTCTACAATGGCAGGGTAT+TGG - Chr5:76120208-76120227 MsG0580028171.01.T01:intron 40.0%
! ACTAAATGAAGTGGACTCTG+TGG + Chr5:76119894-76119913 None:intergenic 40.0%
! ATCTAAAAATGGGAGTGCAC+CGG - Chr5:76120466-76120485 MsG0580028171.01.T01:intron 40.0%
! ATGTAGATTGTAGTCTTGGG+AGG - Chr5:76121378-76121397 MsG0580028171.01.T01:intron 40.0%
! CAGCAAAGGCCTCTAAAAAT+GGG - Chr5:76120906-76120925 MsG0580028171.01.T01:CDS 40.0%
! GAAGTATCAGCTTTCTTTGC+CGG + Chr5:76120488-76120507 None:intergenic 40.0%
! TGAGTTTAGCTCAGTTGGTT+GGG + Chr5:76121116-76121135 None:intergenic 40.0%
! TTCTACAATGGCAGGGTATT+GGG - Chr5:76120209-76120228 MsG0580028171.01.T01:intron 40.0%
!! TGTTGGTTCTGCTATATGCA+AGG - Chr5:76121410-76121429 MsG0580028171.01.T01:intron 40.0%
!!! CTTTTTTTTTGGTAGTGGCC+GGG - Chr5:76121054-76121073 MsG0580028171.01.T01:intron 40.0%
!!! TTTTTTTTTGGTAGTGGCCG+GGG - Chr5:76121055-76121074 MsG0580028171.01.T01:intron 40.0%
GATGGAACAACTTTAGCTGC+AGG + Chr5:76120449-76120468 None:intergenic 45.0%
GCACTATCAGCTTTCTTTGC+GGG + Chr5:76120708-76120727 None:intergenic 45.0%
! ATTGTAGTCTTGGGAGGCAA+TGG - Chr5:76121384-76121403 MsG0580028171.01.T01:intron 45.0%
! GAAGGAACAACTTTTGCTGC+AGG + Chr5:76120670-76120689 None:intergenic 45.0%
! GCAGCAAAGGCCTCTAAAAA+TGG - Chr5:76120905-76120924 MsG0580028171.01.T01:CDS 45.0%
! GTGAGTTTAGCTCAGTTGGT+TGG + Chr5:76121117-76121136 None:intergenic 45.0%
! TTGCGGGTACACTCCATTTT+TGG + Chr5:76120692-76120711 None:intergenic 45.0%
! TTGTAGTCTTGGGAGGCAAT+GGG - Chr5:76121385-76121404 MsG0580028171.01.T01:intron 45.0%
!! GGGTACACTCCATTTTTGGA+AGG + Chr5:76120688-76120707 None:intergenic 45.0%
!! GGTGCACTCCCATTTTTAGA+TGG + Chr5:76120467-76120486 None:intergenic 45.0%
AAAGGCAGCAGTATGGCTTC+TGG - Chr5:76119864-76119883 MsG0580028171.01.T01:CDS 50.0%
AAGGTTCAGGGTTCAAACCC+CGG + Chr5:76121075-76121094 None:intergenic 50.0%
AGAAGCCATACTGCTGCCTT+TGG + Chr5:76119865-76119884 None:intergenic 50.0%
AGGTGTGGAAGAGATGAGAC+GGG + Chr5:76121510-76121529 None:intergenic 50.0%
ATGCAAGGCAGCAGTATCCA+AGG - Chr5:76121425-76121444 MsG0580028171.01.T01:intron 50.0%
CCAACTGAGCTAAACTCACG+AGG - Chr5:76121118-76121137 MsG0580028171.01.T01:intron 50.0%
CTCTTCCAAAGGCAGCAGTA+TGG - Chr5:76119857-76119876 MsG0580028171.01.T01:CDS 50.0%
GAGGTGTGGAAGAGATGAGA+CGG + Chr5:76121511-76121530 None:intergenic 50.0%
GCTGCAAGTTTCTGAGCCAA+AGG + Chr5:76120890-76120909 None:intergenic 50.0%
GGCTCAGAAACTTGCAGCAA+AGG - Chr5:76120892-76120911 MsG0580028171.01.T01:intron 50.0%
TGCAAGGCAGCAGTATCCAA+GGG - Chr5:76121426-76121445 MsG0580028171.01.T01:intron 50.0%
! CCTCGTGAGTTTAGCTCAGT+TGG + Chr5:76121121-76121140 None:intergenic 50.0%
! TTGGGAGGCAATGGGTTTGT+TGG - Chr5:76121393-76121412 MsG0580028171.01.T01:intron 50.0%
!! ACGGGTTGATCGAAGTGCAT+AGG - Chr5:76120162-76120181 MsG0580028171.01.T01:intron 50.0%
!! GGGTGCACTCCCATTTTTAG+AGG + Chr5:76120918-76120937 None:intergenic 50.0%
GGTGTGGAAGAGATGAGACG+GGG + Chr5:76121509-76121528 None:intergenic 55.0%
GTGTGGAAGAGATGAGACGG+GGG + Chr5:76121508-76121527 None:intergenic 55.0%
! AGAGATGAGACGGGGGTATG+TGG + Chr5:76121501-76121520 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr5 gene 76119834 76121620 76119834 ID=MsG0580028171.01;Name=MsG0580028171.01
Chr5 mRNA 76119834 76121620 76119834 ID=MsG0580028171.01.T01;Parent=MsG0580028171.01;Name=MsG0580028171.01.T01;_AED=0.50;_eAED=0.94;_QI=0|0|0|1|0.66|0.57|7|0|243
Chr5 exon 76121540 76121620 76121540 ID=MsG0580028171.01.T01:exon:15481;Parent=MsG0580028171.01.T01
Chr5 exon 76121159 76121365 76121159 ID=MsG0580028171.01.T01:exon:15480;Parent=MsG0580028171.01.T01
Chr5 exon 76120893 76121011 76120893 ID=MsG0580028171.01.T01:exon:15479;Parent=MsG0580028171.01.T01
Chr5 exon 76120675 76120780 76120675 ID=MsG0580028171.01.T01:exon:15478;Parent=MsG0580028171.01.T01
Chr5 exon 76120481 76120555 76120481 ID=MsG0580028171.01.T01:exon:15477;Parent=MsG0580028171.01.T01
Chr5 exon 76119978 76120070 76119978 ID=MsG0580028171.01.T01:exon:15476;Parent=MsG0580028171.01.T01
Chr5 exon 76119834 76119884 76119834 ID=MsG0580028171.01.T01:exon:15475;Parent=MsG0580028171.01.T01
Chr5 CDS 76121540 76121620 76121540 ID=MsG0580028171.01.T01:cds;Parent=MsG0580028171.01.T01
Chr5 CDS 76121159 76121365 76121159 ID=MsG0580028171.01.T01:cds;Parent=MsG0580028171.01.T01
Chr5 CDS 76120893 76121011 76120893 ID=MsG0580028171.01.T01:cds;Parent=MsG0580028171.01.T01
Chr5 CDS 76120675 76120780 76120675 ID=MsG0580028171.01.T01:cds;Parent=MsG0580028171.01.T01
Chr5 CDS 76120481 76120555 76120481 ID=MsG0580028171.01.T01:cds;Parent=MsG0580028171.01.T01
Chr5 CDS 76119978 76120070 76119978 ID=MsG0580028171.01.T01:cds;Parent=MsG0580028171.01.T01
Chr5 CDS 76119834 76119884 76119834 ID=MsG0580028171.01.T01:cds;Parent=MsG0580028171.01.T01
Gene Sequence

>MsG0580028171.01.T01

ATGCCTCAAGAACAATACAATGGCTCTTCCAAAGGCAGCAGTATGGCTTCTGGTTCTCCACAGAGTCCACTTCATTTAGTTAATGATGTTCGTGTGCATGATTACATGGCTTCACAGCCAGATATAAATGCTAAGATGAGATCAATTCTTATTGACGGGTTGATCGAAGTGCATAGGAAATTTGAACTTATGCCGGAAAATTTCTACAATGGCAGGGTATTGGGAGAAAGTCTTGATTTGATCAAATATGTAAATGCTAACTTTAAGGGAACACCTATGTTTCCCAATAAACCTGCAGCTAAAGTTGTTCCATCTAAAAATGGGAGTGCACCGGCAAAGAAAGCTGATACTTCTGACGAAGATTCTGATGAATCTTCTGATGAAGATGAGGTATTAGACATTTTGAACAAAAGTTGTTCCTTCCAAAAATGGAGTGTACCCGCAAAGAAAGCTGATAGTGCTGATAGTTCTTCCGATGACGACTCTGATGAATCTTCTAACGAAGAAGATAAGAAACTTGCAGCAAAGGCCTCTAAAAATGGGAGTGCACCCACAAAGAAAGCTTCAAATGAATCTGATGAAGATGAAATTGTAGTCTTGGGAGGCAATGGGTTTGTTGGTTCTGCTATATGCAAGGCAGCAGTATCCAAGGGCATAGAAATCATAAGCTTAAACAGGTTTGTCAGGACGTTCTACTTACTCAGATTCATGGATAGTGTTGGTACTACATAG
Protein sequence

>MsG0580028171.01.T01

MPQEQYNGSSKGSSMASGSPQSPLHLVNDVRVHDYMASQPDINAKMRSILIDGLIEVHRKFELMPENFYNGRVLGESLDLIKYVNANFKGTPMFPNKPAAKVVPSKNGSAPAKKADTSDEDSDESSDEDEVLDILNKSCSFQKWSVPAKKADSADSSSDDDSDESSNEEDKKLAAKASKNGSAPTKKASNESDEDEIVVLGGNGFVGSAICKAAVSKGIEIISLNRFVRTFYLLRFMDSVGTT*