Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047264.01.T01 | XP_039689256.1 | 97.403 | 77 | 2 | 0 | 1 | 77 | 1 | 77 | 2.11E-44 | 147 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047264.01.T01 | Q9M0N8 | 73.913 | 69 | 17 | 1 | 8 | 76 | 1 | 68 | 1.47E-30 | 105 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047264.01.T01 | A0A072VE60 | 97.297 | 74 | 2 | 0 | 4 | 77 | 33 | 106 | 9.10e-44 | 146 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0880046645.01 | MsG0880047264.01 | -0.801814 | 7.540719e-49 | 3.635599e-46 |
| MsG0780040576.01 | MsG0880047264.01 | 0.804513 | 2.083381e-49 | 1.076222e-46 |
| MsG0480022615.01 | MsG0880047264.01 | 0.835403 | 1.712694e-56 | 2.092249e-53 |
| MsG0580024928.01 | MsG0880047264.01 | 0.812007 | 5.264167e-51 | 3.308238e-48 |
| MsG0580029925.01 | MsG0880047264.01 | -0.802667 | 5.031756e-49 | 2.479173e-46 |
| MsG0780039867.01 | MsG0880047264.01 | 0.806557 | 7.762002e-50 | 4.225928e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047264.01.T01 | MTR_2g008230 | 97.297 | 74 | 2 | 0 | 4 | 77 | 33 | 106 | 2.31e-47 | 146 |
| MsG0880047264.01.T01 | MTR_4g124060 | 97.143 | 70 | 2 | 0 | 8 | 77 | 1 | 70 | 1.47e-43 | 135 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047264.01.T01 | AT4G09550 | 73.913 | 69 | 17 | 1 | 8 | 76 | 1 | 68 | 1.50e-31 | 105 |
| MsG0880047264.01.T01 | AT4G09550 | 73.913 | 69 | 17 | 1 | 8 | 76 | 1 | 68 | 1.50e-31 | 105 |
| MsG0880047264.01.T01 | AT1G73790 | 72.414 | 58 | 16 | 0 | 8 | 65 | 1 | 58 | 3.81e-27 | 94.0 |
Find 24 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGTGCACTTCTTGCAGCTTC+AGG | 0.145529 | 8:-83956505 | None:intergenic |
| ACAAAATACATTCATGATTT+AGG | 0.172021 | 8:-83956706 | None:intergenic |
| CTCATTGCCCTCTGTGATCT+TGG | 0.190398 | 8:+83956601 | MsG0880047264.01.T01:CDS |
| TCATTGCCCTCTGTGATCTT+GGG | 0.281322 | 8:+83956602 | MsG0880047264.01.T01:CDS |
| ACAATAGCAGCAAGTGCTTC+AGG | 0.324395 | 8:-83956631 | None:intergenic |
| TAAACCCGTGTCGAGTATAT+TGG | 0.366888 | 8:-83956557 | None:intergenic |
| AAGAAGTGCACGAGAATCTT+TGG | 0.388040 | 8:+83956516 | MsG0880047264.01.T01:CDS |
| GTGCACTTCTTGCAGCTTCA+GGG | 0.392834 | 8:-83956504 | None:intergenic |
| CATTGCCCTCTGTGATCTTG+GGG | 0.494922 | 8:+83956603 | MsG0880047264.01.T01:CDS |
| TAGGATGCAATGATGACATT+GGG | 0.500215 | 8:-83956678 | None:intergenic |
| TATTGTCAAGGAACTTAGAA+AGG | 0.527792 | 8:+83956648 | MsG0880047264.01.T01:CDS |
| ATTCATGATTTAGGAGGCGT+AGG | 0.534552 | 8:-83956697 | None:intergenic |
| AAATACATTCATGATTTAGG+AGG | 0.583464 | 8:-83956703 | None:intergenic |
| TATTGGACATGTGAAATGCC+AGG | 0.592806 | 8:-83956540 | None:intergenic |
| GTAGGATGCAATGATGACAT+TGG | 0.595456 | 8:-83956679 | None:intergenic |
| TGCACGAGAATCTTTGGACC+TGG | 0.595713 | 8:+83956522 | MsG0880047264.01.T01:CDS |
| CATGTCCAATATACTCGACA+CGG | 0.596616 | 8:+83956552 | MsG0880047264.01.T01:CDS |
| GCACATCTCAGCTTTAAGCA+TGG | 0.611025 | 8:+83956480 | MsG0880047264.01.T01:CDS |
| AGCACTTGCTGCTATTGTCA+AGG | 0.625051 | 8:+83956636 | MsG0880047264.01.T01:CDS |
| TCAAGGAACTTAGAAAGGAG+AGG | 0.641589 | 8:+83956653 | MsG0880047264.01.T01:CDS |
| ATTGACCCCAAGATCACAGA+GGG | 0.646842 | 8:-83956608 | None:intergenic |
| TGAGAATAGAAAGTGCATGA+CGG | 0.680398 | 8:-83956582 | None:intergenic |
| ATGTCCAATATACTCGACAC+GGG | 0.686297 | 8:+83956553 | MsG0880047264.01.T01:CDS |
| GATTGACCCCAAGATCACAG+AGG | 0.775929 | 8:-83956609 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TATTGTCAAGGAACTTAGAA+AGG | + | Chr8:83956648-83956667 | MsG0880047264.01.T01:CDS | 30.0% | |
| TAGGATGCAATGATGACATT+GGG | - | Chr8:83956681-83956700 | None:intergenic | 35.0% | |
| ! | TGAGAATAGAAAGTGCATGA+CGG | - | Chr8:83956585-83956604 | None:intergenic | 35.0% |
| ATGTCCAATATACTCGACAC+GGG | + | Chr8:83956553-83956572 | MsG0880047264.01.T01:CDS | 40.0% | |
| CATGTCCAATATACTCGACA+CGG | + | Chr8:83956552-83956571 | MsG0880047264.01.T01:CDS | 40.0% | |
| GTAGGATGCAATGATGACAT+TGG | - | Chr8:83956682-83956701 | None:intergenic | 40.0% | |
| TAAACCCGTGTCGAGTATAT+TGG | - | Chr8:83956560-83956579 | None:intergenic | 40.0% | |
| TATTGGACATGTGAAATGCC+AGG | - | Chr8:83956543-83956562 | None:intergenic | 40.0% | |
| TCAAGGAACTTAGAAAGGAG+AGG | + | Chr8:83956653-83956672 | MsG0880047264.01.T01:CDS | 40.0% | |
| ! | AAGAAGTGCACGAGAATCTT+TGG | + | Chr8:83956516-83956535 | MsG0880047264.01.T01:CDS | 40.0% |
| ACAATAGCAGCAAGTGCTTC+AGG | - | Chr8:83956634-83956653 | None:intergenic | 45.0% | |
| ATTGACCCCAAGATCACAGA+GGG | - | Chr8:83956611-83956630 | None:intergenic | 45.0% | |
| TCATTGCCCTCTGTGATCTT+GGG | + | Chr8:83956602-83956621 | MsG0880047264.01.T01:CDS | 45.0% | |
| ! | AGCACTTGCTGCTATTGTCA+AGG | + | Chr8:83956636-83956655 | MsG0880047264.01.T01:CDS | 45.0% |
| !! | GCACATCTCAGCTTTAAGCA+TGG | + | Chr8:83956480-83956499 | MsG0880047264.01.T01:CDS | 45.0% |
| CATTGCCCTCTGTGATCTTG+GGG | + | Chr8:83956603-83956622 | MsG0880047264.01.T01:CDS | 50.0% | |
| CTCATTGCCCTCTGTGATCT+TGG | + | Chr8:83956601-83956620 | MsG0880047264.01.T01:CDS | 50.0% | |
| GATTGACCCCAAGATCACAG+AGG | - | Chr8:83956612-83956631 | None:intergenic | 50.0% | |
| GTGCACTTCTTGCAGCTTCA+GGG | - | Chr8:83956507-83956526 | None:intergenic | 50.0% | |
| TGCACGAGAATCTTTGGACC+TGG | + | Chr8:83956522-83956541 | MsG0880047264.01.T01:CDS | 50.0% | |
| CGTGCACTTCTTGCAGCTTC+AGG | - | Chr8:83956508-83956527 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 83956478 | 83956717 | 83956478 | ID=MsG0880047264.01;Name=MsG0880047264.01 |
| Chr8 | mRNA | 83956478 | 83956717 | 83956478 | ID=MsG0880047264.01.T01;Parent=MsG0880047264.01;Name=MsG0880047264.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|79 |
| Chr8 | exon | 83956478 | 83956717 | 83956478 | ID=MsG0880047264.01.T01:exon:1519;Parent=MsG0880047264.01.T01 |
| Chr8 | CDS | 83956478 | 83956717 | 83956478 | ID=MsG0880047264.01.T01:cds;Parent=MsG0880047264.01.T01 |
| Gene Sequence |
| Protein sequence |
