AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880046645.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046645.01.T01 MTR_4g112370 97.308 260 2 2 1 260 6 260 0.0 518
MsG0880046645.01.T01 MTR_2g055990 73.622 254 56 4 16 260 8 259 4.91e-135 381
MsG0880046645.01.T01 MTR_2g054380 54.231 260 98 5 18 260 15 270 3.51e-86 258
MsG0880046645.01.T01 MTR_1g053130 52.153 209 78 4 74 260 76 284 1.82e-60 192
MsG0880046645.01.T01 MTR_3g094830 44.693 179 93 4 84 260 85 259 2.48e-52 171
MsG0880046645.01.T01 MTR_8g040690 46.108 167 85 2 95 260 85 247 3.22e-52 170
MsG0880046645.01.T01 MTR_8g040680 45.509 167 86 2 95 260 95 257 2.70e-50 166
MsG0880046645.01.T01 MTR_3g101300 46.821 173 77 6 96 259 69 235 4.14e-45 152
MsG0880046645.01.T01 MTR_7g064810 46.012 163 82 3 98 259 65 222 3.65e-41 141
MsG0880046645.01.T01 MTR_3g086740 37.576 165 97 3 98 259 81 242 9.08e-34 122
MsG0880046645.01.T01 MTR_8g089865 39.157 166 91 5 100 259 59 220 1.31e-32 119
MsG0880046645.01.T01 MTR_1g102920 89.583 48 4 1 41 88 7 53 1.29e-20 83.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046645.01.T01 AT5G23100 58.696 276 85 6 13 260 3 277 5.49e-100 293
MsG0880046645.01.T01 AT3G25640 66.667 180 45 3 92 260 118 293 1.60e-78 239
MsG0880046645.01.T01 AT3G25640 66.667 180 45 3 92 260 92 267 2.81e-78 238
MsG0880046645.01.T01 AT2G41660 52.222 180 70 4 92 260 123 297 2.12e-60 193
MsG0880046645.01.T01 AT2G21990 49.102 167 80 2 95 260 90 252 2.98e-54 176
MsG0880046645.01.T01 AT4G39610 47.059 170 85 2 96 260 95 264 5.96e-54 175
MsG0880046645.01.T01 AT5G06990 46.893 177 78 3 95 260 90 261 1.46e-53 174
MsG0880046645.01.T01 AT2G37880 50.595 168 73 4 98 259 81 244 1.19e-48 161
MsG0880046645.01.T01 AT5G42680 47.561 164 76 5 100 259 79 236 4.76e-42 144
MsG0880046645.01.T01 AT5G42680 47.561 164 76 5 100 259 79 236 4.76e-42 144
MsG0880046645.01.T01 AT1G21050 44.444 162 82 4 100 259 89 244 1.66e-40 140
MsG0880046645.01.T01 AT1G21050 44.444 162 82 4 100 259 125 280 2.50e-40 140
MsG0880046645.01.T01 AT1G76610 43.713 167 78 4 95 259 73 225 9.19e-36 127
MsG0880046645.01.T01 AT5G65340 41.212 165 92 4 100 259 85 249 5.93e-34 123
MsG0880046645.01.T01 AT2G22460 38.889 162 93 2 98 258 85 241 1.28e-31 117

Find 47 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGGTGGGTGATGGTTCTTC+TGG 0.210488 8:-76093967 None:intergenic
TTGGTTCGTGAAATGGCTTC+TGG 0.218997 8:+76094276 MsG0880046645.01.T01:CDS
TCACGAACCAAACCACTAAT+TGG 0.265712 8:-76094264 None:intergenic
GGCCACGACGATAACCAAAA+AGG 0.304815 8:-76094176 None:intergenic
CTTCCAAAAGACACTTTCAT+TGG 0.317781 8:+76093920 MsG0880046645.01.T01:CDS
ACACCAGCACCCATTGAAAT+AGG 0.321507 8:-76094474 None:intergenic
TGGTGGGTGATGGTTCTTCT+GGG 0.369853 8:-76093966 None:intergenic
TAGTGGTTTGGTTCGTGAAA+TGG 0.373367 8:+76094269 MsG0880046645.01.T01:CDS
TCAGGAGCACCATTGCTATC+AGG 0.382273 8:-76094606 None:intergenic
TGGTTCGTGAAATGGCTTCT+GGG 0.390455 8:+76094277 MsG0880046645.01.T01:CDS
TGCAACACCAATTAGTGGTT+TGG 0.390838 8:+76094257 MsG0880046645.01.T01:CDS
GGTCTTGGTTCACGTGTTGT+TGG 0.394137 8:+76094150 MsG0880046645.01.T01:CDS
TGTCCAATGAAAGTGTCTTT+TGG 0.397094 8:-76093923 None:intergenic
GCATGTCCTCCTCCTGAAAC+AGG 0.405991 8:-76094036 None:intergenic
GAGCTTGCAACACCAATTAG+TGG 0.410894 8:+76094252 MsG0880046645.01.T01:CDS
TCAAATCTACCCTCTGGTCT+TGG 0.414007 8:+76094135 MsG0880046645.01.T01:CDS
CTTTGGAGGACTTATTGTAA+TGG 0.414774 8:+76094381 MsG0880046645.01.T01:CDS
GCAAGGTTTGAGAGAATTGT+TGG 0.417883 8:+76094549 MsG0880046645.01.T01:CDS
TTTCATTGGACAAACAAAGT+TGG 0.442917 8:+76093934 MsG0880046645.01.T01:CDS
GATTGTTGCAAGAGCCACTT+TGG 0.458144 8:+76094364 MsG0880046645.01.T01:CDS
TCTTGAATGTGATAAAGTTA+AGG 0.462515 8:+76094314 MsG0880046645.01.T01:CDS
AGCAAATAAACACTAAGCTC+AGG 0.497939 8:-76094624 None:intergenic
GCTGTTGAGCCTATTTCAAT+GGG 0.498481 8:+76094465 MsG0880046645.01.T01:CDS
GAGCCTATTTCAATGGGTGC+TGG 0.499015 8:+76094471 MsG0880046645.01.T01:CDS
AGCTGTTGAGCCTATTTCAA+TGG 0.499933 8:+76094464 MsG0880046645.01.T01:CDS
CAAGGTTTGAGAGAATTGTT+GGG 0.500323 8:+76094550 MsG0880046645.01.T01:CDS
GAACAACCAGCTTCACCACC+TGG 0.519832 8:-76094501 None:intergenic
GATAAAGTTAAGGAGAAAGA+AGG 0.526239 8:+76094324 MsG0880046645.01.T01:CDS
GGTGAAGCTGGTTGTTCTGA+TGG 0.530478 8:+76094507 MsG0880046645.01.T01:CDS
AAGTTATGTATATGAGAGCA+AGG 0.549681 8:+76094532 MsG0880046645.01.T01:CDS
TGTTGCAAGAGCCACTTTGG+AGG 0.550258 8:+76094367 MsG0880046645.01.T01:CDS
AACCGTTCAAATCTACCCTC+TGG 0.551791 8:+76094129 MsG0880046645.01.T01:CDS
GAGAAAAGCTGGTTGTGAAG+TGG 0.553764 8:-76094226 None:intergenic
ACGTATTCTTGAAATTGCTA+CGG 0.556557 8:-76094082 None:intergenic
GAGGAGGACATGCAGCTACA+AGG 0.559240 8:+76094046 MsG0880046645.01.T01:CDS
AGATGGGTCTTGAGGAGTTG+AGG 0.562495 8:-76093899 None:intergenic
GCAAGCTCAATGAGAAAAGC+TGG 0.564770 8:-76094237 None:intergenic
TCGTGAAATGGCTTCTGGGT+TGG 0.566947 8:+76094281 MsG0880046645.01.T01:CDS
AGAAAAGCTGGTTGTGAAGT+GGG 0.576053 8:-76094225 None:intergenic
GACCAGAGGGTAGATTTGAA+CGG 0.580928 8:-76094131 None:intergenic
TCTGAAAAGCAAAATGAACA+TGG 0.632776 8:-76094197 None:intergenic
TTTGGTAATAGAAGAGAATG+TGG 0.634366 8:+76094417 MsG0880046645.01.T01:CDS
TTACAATAAGTCCTCCAAAG+TGG 0.641562 8:-76094378 None:intergenic
ACACGTGAACCAAGACCAGA+GGG 0.658866 8:-76094144 None:intergenic
GAAAAGCTGGTTGTGAAGTG+GGG 0.678282 8:-76094224 None:intergenic
AACACGTGAACCAAGACCAG+AGG 0.689373 8:-76094145 None:intergenic
ATGATGAATCCTGATAGCAA+TGG 0.728571 8:+76094597 MsG0880046645.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TCTTGAATGTGATAAAGTTA+AGG + Chr8:76094314-76094333 MsG0880046645.01.T01:CDS 25.0%
!! AGGTTTTTCTTCATGTTTTT+TGG - Chr8:76094019-76094038 None:intergenic 25.0%
!! TATTGTAATGGAAAAAAGTG+TGG + Chr8:76094393-76094412 MsG0880046645.01.T01:CDS 25.0%
!!! GTTTTGTTCTTCTATGATTT+TGG - Chr8:76093995-76094014 None:intergenic 25.0%
AAGTTATGTATATGAGAGCA+AGG + Chr8:76094532-76094551 MsG0880046645.01.T01:CDS 30.0%
ACGTATTCTTGAAATTGCTA+CGG - Chr8:76094085-76094104 None:intergenic 30.0%
AGAATGTGGTGAAAAAGATT+GGG + Chr8:76094431-76094450 MsG0880046645.01.T01:CDS 30.0%
GATAAAGTTAAGGAGAAAGA+AGG + Chr8:76094324-76094343 MsG0880046645.01.T01:CDS 30.0%
TCTGAAAAGCAAAATGAACA+TGG - Chr8:76094200-76094219 None:intergenic 30.0%
TTTCATTGGACAAACAAAGT+TGG + Chr8:76093934-76093953 MsG0880046645.01.T01:CDS 30.0%
! AATGGAAAAAAGTGTGGATT+TGG + Chr8:76094399-76094418 MsG0880046645.01.T01:CDS 30.0%
! TTCTTCTATGATTTTGGACA+TGG - Chr8:76093989-76094008 None:intergenic 30.0%
! TTTGGTAATAGAAGAGAATG+TGG + Chr8:76094417-76094436 MsG0880046645.01.T01:CDS 30.0%
AGCAAATAAACACTAAGCTC+AGG - Chr8:76094627-76094646 None:intergenic 35.0%
ATGATGAATCCTGATAGCAA+TGG + Chr8:76094597-76094616 MsG0880046645.01.T01:CDS 35.0%
CAAGGTTTGAGAGAATTGTT+GGG + Chr8:76094550-76094569 MsG0880046645.01.T01:CDS 35.0%
CATGAAGAAAAACCTGTTTC+AGG + Chr8:76094024-76094043 MsG0880046645.01.T01:CDS 35.0%
CTTCCAAAAGACACTTTCAT+TGG + Chr8:76093920-76093939 MsG0880046645.01.T01:CDS 35.0%
GAGAATGTGGTGAAAAAGAT+TGG + Chr8:76094430-76094449 MsG0880046645.01.T01:CDS 35.0%
TTACAATAAGTCCTCCAAAG+TGG - Chr8:76094381-76094400 None:intergenic 35.0%
! CTTTGGAGGACTTATTGTAA+TGG + Chr8:76094381-76094400 MsG0880046645.01.T01:CDS 35.0%
! TCTATGATTTTGGACATGGT+GGG - Chr8:76093985-76094004 None:intergenic 35.0%
! TTCTATGATTTTGGACATGG+TGG - Chr8:76093986-76094005 None:intergenic 35.0%
!!! GAAAGTGTCTTTTGGAAGAT+GGG - Chr8:76093918-76093937 None:intergenic 35.0%
!!! TGAAAGTGTCTTTTGGAAGA+TGG - Chr8:76093919-76093938 None:intergenic 35.0%
!!! TGTCCAATGAAAGTGTCTTT+TGG - Chr8:76093926-76093945 None:intergenic 35.0%
AGAAAAGCTGGTTGTGAAGT+GGG - Chr8:76094228-76094247 None:intergenic 40.0%
AGCTGTTGAGCCTATTTCAA+TGG + Chr8:76094464-76094483 MsG0880046645.01.T01:CDS 40.0%
GAAGAAAAACCTGTTTCAGG+AGG + Chr8:76094027-76094046 MsG0880046645.01.T01:CDS 40.0%
GCAAGGTTTGAGAGAATTGT+TGG + Chr8:76094549-76094568 MsG0880046645.01.T01:CDS 40.0%
GCTGTTGAGCCTATTTCAAT+GGG + Chr8:76094465-76094484 MsG0880046645.01.T01:CDS 40.0%
TCACGAACCAAACCACTAAT+TGG - Chr8:76094267-76094286 None:intergenic 40.0%
TGCAACACCAATTAGTGGTT+TGG + Chr8:76094257-76094276 MsG0880046645.01.T01:CDS 40.0%
!! TAGTGGTTTGGTTCGTGAAA+TGG + Chr8:76094269-76094288 MsG0880046645.01.T01:CDS 40.0%
AACCGTTCAAATCTACCCTC+TGG + Chr8:76094129-76094148 MsG0880046645.01.T01:CDS 45.0%
ACACCAGCACCCATTGAAAT+AGG - Chr8:76094477-76094496 None:intergenic 45.0%
GAAAAACCTGTTTCAGGAGG+AGG + Chr8:76094030-76094049 MsG0880046645.01.T01:CDS 45.0%
GAAAAGCTGGTTGTGAAGTG+GGG - Chr8:76094227-76094246 None:intergenic 45.0%
GACCAGAGGGTAGATTTGAA+CGG - Chr8:76094134-76094153 None:intergenic 45.0%
GAGAAAAGCTGGTTGTGAAG+TGG - Chr8:76094229-76094248 None:intergenic 45.0%
GAGCTTGCAACACCAATTAG+TGG + Chr8:76094252-76094271 MsG0880046645.01.T01:CDS 45.0%
GATTGTTGCAAGAGCCACTT+TGG + Chr8:76094364-76094383 MsG0880046645.01.T01:CDS 45.0%
GCAAGCTCAATGAGAAAAGC+TGG - Chr8:76094240-76094259 None:intergenic 45.0%
GCCACGACGATAACCAAAAA+GGG - Chr8:76094178-76094197 None:intergenic 45.0%
TCAAATCTACCCTCTGGTCT+TGG + Chr8:76094135-76094154 MsG0880046645.01.T01:CDS 45.0%
TGGTTCGTGAAATGGCTTCT+GGG + Chr8:76094277-76094296 MsG0880046645.01.T01:CDS 45.0%
! TTGGTTCGTGAAATGGCTTC+TGG + Chr8:76094276-76094295 MsG0880046645.01.T01:CDS 45.0%
!! CGTGTTGTTGGAACCCTTTT+TGG + Chr8:76094162-76094181 MsG0880046645.01.T01:CDS 45.0%
!! CTTTTGGAAGATGGGTCTTG+AGG - Chr8:76093910-76093929 None:intergenic 45.0%
!!! ACCCTTTTTGGTTATCGTCG+TGG + Chr8:76094174-76094193 MsG0880046645.01.T01:CDS 45.0%
!!! ATTTTGGACATGGTGGGTGA+TGG - Chr8:76093979-76093998 None:intergenic 45.0%
AACACGTGAACCAAGACCAG+AGG - Chr8:76094148-76094167 None:intergenic 50.0%
ACACGTGAACCAAGACCAGA+GGG - Chr8:76094147-76094166 None:intergenic 50.0%
AGATGGGTCTTGAGGAGTTG+AGG - Chr8:76093902-76093921 None:intergenic 50.0%
GAGCCTATTTCAATGGGTGC+TGG + Chr8:76094471-76094490 MsG0880046645.01.T01:CDS 50.0%
GGCCACGACGATAACCAAAA+AGG - Chr8:76094179-76094198 None:intergenic 50.0%
GGTCTTGGTTCACGTGTTGT+TGG + Chr8:76094150-76094169 MsG0880046645.01.T01:CDS 50.0%
GGTGAAGCTGGTTGTTCTGA+TGG + Chr8:76094507-76094526 MsG0880046645.01.T01:CDS 50.0%
TCGTGAAATGGCTTCTGGGT+TGG + Chr8:76094281-76094300 MsG0880046645.01.T01:CDS 50.0%
! TGGTGGGTGATGGTTCTTCT+GGG - Chr8:76093969-76093988 None:intergenic 50.0%
! TGTTGCAAGAGCCACTTTGG+AGG + Chr8:76094367-76094386 MsG0880046645.01.T01:CDS 50.0%
!! ATGGTGGGTGATGGTTCTTC+TGG - Chr8:76093970-76093989 None:intergenic 50.0%
!! TCAGGAGCACCATTGCTATC+AGG - Chr8:76094609-76094628 None:intergenic 50.0%
GAACAACCAGCTTCACCACC+TGG - Chr8:76094504-76094523 None:intergenic 55.0%
GAGGAGGACATGCAGCTACA+AGG + Chr8:76094046-76094065 MsG0880046645.01.T01:CDS 55.0%
GCATGTCCTCCTCCTGAAAC+AGG - Chr8:76094039-76094058 None:intergenic 55.0%
! GTTTTGCCAGGTGGTGAAGC+TGG + Chr8:76094495-76094514 MsG0880046645.01.T01:CDS 55.0%
!!! ATGGGTGCTGGTGTTTTGCC+AGG + Chr8:76094483-76094502 MsG0880046645.01.T01:CDS 55.0%
!!! GGTGCTGGTGTTTTGCCAGG+TGG + Chr8:76094486-76094505 MsG0880046645.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 76093874 76094656 76093874 ID=MsG0880046645.01;Name=MsG0880046645.01
Chr8 mRNA 76093874 76094656 76093874 ID=MsG0880046645.01.T01;Parent=MsG0880046645.01;Name=MsG0880046645.01.T01;_AED=0.30;_eAED=0.30;_QI=0|-1|0|1|-1|1|1|0|260
Chr8 exon 76093874 76094656 76093874 ID=MsG0880046645.01.T01:exon:8294;Parent=MsG0880046645.01.T01
Chr8 CDS 76093874 76094656 76093874 ID=MsG0880046645.01.T01:cds;Parent=MsG0880046645.01.T01
Gene Sequence

>MsG0880046645.01.T01

ATGGAAGACACAAACACAAGCACAACCTCAACTCCTCAAGACCCATCTTCCAAAAGACACTTTCATTGGACAAACAAAGTTGGAAATGAAGACCCAGAAGAACCATCACCCACCATGTCCAAAATCATAGAAGAACAAAACACCAAAAAACATGAAGAAAAACCTGTTTCAGGAGGAGGACATGCAGCTACAAGGAAGAAGCTACAAACCGTAGCAATTTCAAGAATACGTTCTGTTTTAACAGTTTTCAGCAAAAACCGTTCAAATCTACCCTCTGGTCTTGGTTCACGTGTTGTTGGAACCCTTTTTGGTTATCGTCGTGGCCATGTTCATTTTGCTTTTCAGAAAGACCCCACTTCACAACCAGCTTTTCTCATTGAGCTTGCAACACCAATTAGTGGTTTGGTTCGTGAAATGGCTTCTGGGTTGGTACGAATTGCTCTTGAATGTGATAAAGTTAAGGAGAAAGAAGGTGAGAAGAAGAGTTTGAGATTGTTGCAAGAGCCACTTTGGAGGACTTATTGTAATGGAAAAAAGTGTGGATTTGGTAATAGAAGAGAATGTGGTGAAAAAGATTGGGAGATTTTGAAAGCTGTTGAGCCTATTTCAATGGGTGCTGGTGTTTTGCCAGGTGGTGAAGCTGGTTGTTCTGATGGTGAAGTTATGTATATGAGAGCAAGGTTTGAGAGAATTGTTGGGTCTAGAGATTCTGAAGCTTTCTATATGATGAATCCTGATAGCAATGGTGCTCCTGAGCTTAGTGTTTATTTGCTTAGAGTTTAA

Protein sequence

>MsG0880046645.01.T01

MEDTNTSTTSTPQDPSSKRHFHWTNKVGNEDPEEPSPTMSKIIEEQNTKKHEEKPVSGGGHAATRKKLQTVAISRIRSVLTVFSKNRSNLPSGLGSRVVGTLFGYRRGHVHFAFQKDPTSQPAFLIELATPISGLVREMASGLVRIALECDKVKEKEGEKKSLRLLQEPLWRTYCNGKKCGFGNRRECGEKDWEILKAVEPISMGAGVLPGGEAGCSDGEVMYMRARFERIVGSRDSEAFYMMNPDSNGAPELSVYLLRV*