Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047495.01.T01 | XP_003610153.1 | 96.516 | 287 | 8 | 1 | 1 | 285 | 1 | 287 | 0 | 571 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047495.01.T01 | Q38910 | 75.352 | 284 | 66 | 3 | 1 | 283 | 3 | 283 | 1.80E-157 | 442 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047495.01.T01 | G7JD12 | 96.516 | 287 | 8 | 1 | 1 | 285 | 1 | 287 | 0.0 | 571 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0880045477.01 | MsG0880047495.01 | 0.805951 | 1.041550e-49 | 5.583555e-47 |
| MsG0880047123.01 | MsG0880047495.01 | 0.808291 | 3.329680e-50 | 1.896516e-47 |
| MsG0880047494.01 | MsG0880047495.01 | 0.875104 | 4.006299e-68 | 1.848207e-64 |
| MsG0380011745.01 | MsG0880047495.01 | 0.803229 | 3.851793e-49 | 1.925192e-46 |
| MsG0580025441.01 | MsG0880047495.01 | 0.822883 | 1.866346e-53 | 1.582017e-50 |
| MsG0380015676.01 | MsG0880047495.01 | 0.819913 | 9.045046e-53 | 7.053734e-50 |
| MsG0380015862.01 | MsG0880047495.01 | 0.825274 | 5.126414e-54 | 4.650900e-51 |
| MsG0380017650.01 | MsG0880047495.01 | 0.800098 | 1.689847e-48 | 7.800776e-46 |
| MsG0280009139.01 | MsG0880047495.01 | 0.814799 | 1.281397e-51 | 8.682349e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047495.01.T01 | MTR_4g128580 | 96.516 | 287 | 8 | 1 | 1 | 285 | 1 | 287 | 0.0 | 571 |
| MsG0880047495.01.T01 | MTR_4g128410 | 81.720 | 279 | 49 | 2 | 5 | 283 | 4 | 280 | 7.10e-171 | 474 |
| MsG0880047495.01.T01 | MTR_7g093530 | 83.630 | 281 | 41 | 3 | 3 | 283 | 4 | 279 | 3.65e-167 | 464 |
| MsG0880047495.01.T01 | MTR_4g128570 | 76.596 | 282 | 50 | 1 | 3 | 284 | 8 | 273 | 4.97e-159 | 444 |
| MsG0880047495.01.T01 | MTR_4g126920 | 77.358 | 265 | 59 | 1 | 20 | 284 | 19 | 282 | 2.90e-151 | 424 |
| MsG0880047495.01.T01 | MTR_4g128400 | 73.333 | 285 | 72 | 3 | 1 | 283 | 1 | 283 | 8.41e-150 | 421 |
| MsG0880047495.01.T01 | MTR_4g128590 | 72.664 | 289 | 48 | 5 | 8 | 285 | 9 | 277 | 2.42e-147 | 414 |
| MsG0880047495.01.T01 | MTR_2g038860 | 70.609 | 279 | 77 | 2 | 9 | 283 | 9 | 286 | 6.81e-145 | 409 |
| MsG0880047495.01.T01 | MTR_2g038705 | 70.609 | 279 | 77 | 2 | 9 | 283 | 9 | 286 | 6.81e-145 | 409 |
| MsG0880047495.01.T01 | MTR_8g081040 | 68.966 | 290 | 78 | 3 | 1 | 285 | 1 | 283 | 7.58e-143 | 403 |
| MsG0880047495.01.T01 | MTR_3g084620 | 70.175 | 285 | 75 | 2 | 1 | 283 | 1 | 277 | 9.70e-142 | 400 |
| MsG0880047495.01.T01 | MTR_3g084630 | 70.175 | 285 | 75 | 2 | 1 | 283 | 1 | 277 | 1.12e-141 | 400 |
| MsG0880047495.01.T01 | MTR_8g102220 | 66.541 | 266 | 67 | 3 | 19 | 283 | 22 | 266 | 5.40e-125 | 357 |
| MsG0880047495.01.T01 | MTR_8g102910 | 54.152 | 277 | 121 | 3 | 10 | 283 | 6 | 279 | 6.69e-105 | 307 |
| MsG0880047495.01.T01 | MTR_6g088320 | 52.448 | 286 | 130 | 4 | 1 | 283 | 1 | 283 | 1.10e-102 | 301 |
| MsG0880047495.01.T01 | MTR_5g091730 | 53.333 | 285 | 124 | 3 | 1 | 283 | 1 | 278 | 1.16e-102 | 301 |
| MsG0880047495.01.T01 | MTR_6g033085 | 56.538 | 260 | 111 | 2 | 26 | 285 | 32 | 289 | 3.50e-101 | 298 |
| MsG0880047495.01.T01 | MTR_7g084770 | 52.249 | 289 | 130 | 3 | 1 | 283 | 1 | 287 | 3.94e-100 | 295 |
| MsG0880047495.01.T01 | MTR_6g033055 | 55.769 | 260 | 113 | 2 | 26 | 285 | 32 | 289 | 3.98e-100 | 295 |
| MsG0880047495.01.T01 | MTR_7g084760 | 56.202 | 258 | 111 | 2 | 26 | 283 | 32 | 287 | 8.73e-100 | 294 |
| MsG0880047495.01.T01 | MTR_4g097640 | 50.538 | 279 | 131 | 4 | 9 | 283 | 12 | 287 | 3.41e-99 | 293 |
| MsG0880047495.01.T01 | MTR_4g097670 | 50.538 | 279 | 131 | 4 | 9 | 283 | 12 | 287 | 3.41e-99 | 293 |
| MsG0880047495.01.T01 | MTR_7g084750 | 55.039 | 258 | 114 | 2 | 26 | 283 | 32 | 287 | 2.09e-98 | 291 |
| MsG0880047495.01.T01 | MTR_3g089540 | 53.053 | 262 | 116 | 3 | 23 | 283 | 27 | 282 | 4.15e-98 | 290 |
| MsG0880047495.01.T01 | MTR_4g097700 | 50.958 | 261 | 124 | 3 | 24 | 283 | 67 | 324 | 2.76e-95 | 284 |
| MsG0880047495.01.T01 | MTR_2g089140 | 47.260 | 292 | 141 | 5 | 1 | 284 | 1 | 287 | 1.99e-93 | 278 |
| MsG0880047495.01.T01 | MTR_4g045853 | 48.601 | 286 | 131 | 6 | 10 | 285 | 11 | 290 | 4.42e-93 | 277 |
| MsG0880047495.01.T01 | MTR_1g105900 | 46.622 | 296 | 142 | 5 | 1 | 284 | 1 | 292 | 2.01e-92 | 276 |
| MsG0880047495.01.T01 | MTR_1g105495 | 45.704 | 291 | 147 | 5 | 3 | 284 | 7 | 295 | 2.63e-86 | 260 |
| MsG0880047495.01.T01 | MTR_2g089140 | 51.327 | 226 | 100 | 3 | 67 | 284 | 1 | 224 | 2.27e-81 | 245 |
| MsG0880047495.01.T01 | MTR_8g013055 | 44.867 | 263 | 136 | 4 | 26 | 285 | 31 | 287 | 2.94e-76 | 234 |
| MsG0880047495.01.T01 | MTR_7g056090 | 41.727 | 278 | 151 | 7 | 7 | 283 | 14 | 281 | 3.92e-64 | 203 |
| MsG0880047495.01.T01 | MTR_7g102260 | 40.000 | 280 | 149 | 7 | 13 | 284 | 24 | 292 | 3.77e-62 | 198 |
| MsG0880047495.01.T01 | MTR_4g057450 | 41.573 | 267 | 137 | 7 | 25 | 283 | 49 | 304 | 5.22e-61 | 196 |
| MsG0880047495.01.T01 | MTR_1g063020 | 37.457 | 291 | 163 | 7 | 1 | 283 | 15 | 294 | 1.08e-59 | 192 |
| MsG0880047495.01.T01 | MTR_2g095800 | 40.520 | 269 | 138 | 8 | 25 | 283 | 53 | 309 | 1.09e-59 | 192 |
| MsG0880047495.01.T01 | MTR_2g049130 | 39.655 | 290 | 159 | 8 | 1 | 285 | 3 | 281 | 5.47e-59 | 190 |
| MsG0880047495.01.T01 | MTR_2g049030 | 37.370 | 289 | 158 | 9 | 5 | 281 | 4 | 281 | 7.97e-58 | 187 |
| MsG0880047495.01.T01 | MTR_1g033420 | 39.326 | 267 | 147 | 8 | 26 | 284 | 33 | 292 | 1.01e-53 | 177 |
| MsG0880047495.01.T01 | MTR_8g064180 | 36.431 | 269 | 148 | 7 | 30 | 283 | 50 | 310 | 5.84e-50 | 167 |
| MsG0880047495.01.T01 | MTR_5g029100 | 35.448 | 268 | 159 | 8 | 26 | 283 | 42 | 305 | 2.49e-47 | 162 |
| MsG0880047495.01.T01 | MTR_8g090100 | 35.531 | 273 | 151 | 11 | 26 | 284 | 21 | 282 | 2.47e-42 | 148 |
| MsG0880047495.01.T01 | MTR_5g029100 | 34.118 | 255 | 154 | 8 | 39 | 283 | 24 | 274 | 7.00e-42 | 146 |
| MsG0880047495.01.T01 | MTR_8g479340 | 45.098 | 102 | 45 | 6 | 188 | 284 | 41 | 136 | 2.11e-19 | 82.8 |
| MsG0880047495.01.T01 | MTR_8g032640 | 33.333 | 177 | 110 | 4 | 45 | 219 | 30 | 200 | 3.34e-16 | 75.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047495.01.T01 | AT4G25810 | 75.352 | 284 | 66 | 3 | 1 | 283 | 3 | 283 | 1.84e-158 | 442 |
| MsG0880047495.01.T01 | AT5G57560 | 73.214 | 280 | 70 | 3 | 8 | 283 | 3 | 281 | 1.64e-155 | 435 |
| MsG0880047495.01.T01 | AT5G57550 | 72.243 | 263 | 68 | 2 | 23 | 285 | 27 | 284 | 9.40e-147 | 413 |
| MsG0880047495.01.T01 | AT4G30270 | 69.675 | 277 | 68 | 2 | 8 | 284 | 6 | 266 | 1.03e-144 | 407 |
| MsG0880047495.01.T01 | AT5G48070 | 65.233 | 279 | 88 | 4 | 7 | 284 | 12 | 282 | 2.42e-139 | 394 |
| MsG0880047495.01.T01 | AT2G18800 | 64.746 | 295 | 90 | 4 | 4 | 284 | 3 | 297 | 7.55e-139 | 394 |
| MsG0880047495.01.T01 | AT3G23730 | 66.906 | 278 | 86 | 3 | 11 | 283 | 10 | 286 | 2.79e-136 | 387 |
| MsG0880047495.01.T01 | AT4G14130 | 65.603 | 282 | 96 | 1 | 2 | 283 | 4 | 284 | 1.16e-134 | 382 |
| MsG0880047495.01.T01 | AT5G57530 | 67.164 | 268 | 78 | 3 | 20 | 283 | 21 | 282 | 8.52e-132 | 375 |
| MsG0880047495.01.T01 | AT4G30280 | 64.211 | 285 | 93 | 4 | 3 | 284 | 4 | 282 | 3.29e-129 | 369 |
| MsG0880047495.01.T01 | AT5G57540 | 65.779 | 263 | 84 | 2 | 23 | 283 | 23 | 281 | 2.70e-128 | 366 |
| MsG0880047495.01.T01 | AT4G30290 | 64.211 | 285 | 93 | 4 | 1 | 284 | 1 | 277 | 3.24e-128 | 366 |
| MsG0880047495.01.T01 | AT4G25820 | 66.038 | 265 | 88 | 1 | 19 | 283 | 23 | 285 | 1.25e-126 | 362 |
| MsG0880047495.01.T01 | AT1G65310 | 65.299 | 268 | 86 | 3 | 18 | 284 | 43 | 304 | 2.22e-126 | 362 |
| MsG0880047495.01.T01 | AT1G65310 | 65.299 | 268 | 86 | 3 | 18 | 284 | 21 | 282 | 2.55e-126 | 361 |
| MsG0880047495.01.T01 | AT5G13870 | 53.448 | 290 | 119 | 4 | 3 | 283 | 5 | 287 | 6.38e-100 | 295 |
| MsG0880047495.01.T01 | AT5G13870 | 53.448 | 290 | 119 | 4 | 3 | 283 | 5 | 287 | 6.38e-100 | 295 |
| MsG0880047495.01.T01 | AT5G13870 | 53.448 | 290 | 119 | 4 | 3 | 283 | 5 | 287 | 6.38e-100 | 295 |
| MsG0880047495.01.T01 | AT5G65730 | 47.518 | 282 | 143 | 4 | 4 | 283 | 12 | 290 | 6.28e-97 | 287 |
| MsG0880047495.01.T01 | AT4G28850 | 48.097 | 289 | 141 | 5 | 1 | 284 | 4 | 288 | 3.69e-94 | 280 |
| MsG0880047495.01.T01 | AT2G06850 | 50.177 | 283 | 134 | 3 | 1 | 283 | 15 | 290 | 3.99e-94 | 280 |
| MsG0880047495.01.T01 | AT4G37800 | 48.571 | 280 | 140 | 3 | 6 | 284 | 14 | 290 | 4.12e-94 | 280 |
| MsG0880047495.01.T01 | AT4G03210 | 48.043 | 281 | 140 | 4 | 7 | 285 | 10 | 286 | 6.08e-93 | 277 |
| MsG0880047495.01.T01 | AT2G14620 | 48.551 | 276 | 134 | 5 | 14 | 284 | 23 | 295 | 1.07e-88 | 266 |
| MsG0880047495.01.T01 | AT2G06850 | 56.140 | 228 | 98 | 2 | 56 | 283 | 5 | 230 | 2.70e-88 | 263 |
| MsG0880047495.01.T01 | AT4G03210 | 53.200 | 250 | 108 | 5 | 38 | 285 | 16 | 258 | 4.50e-88 | 263 |
| MsG0880047495.01.T01 | AT1G11545 | 46.959 | 296 | 137 | 8 | 2 | 284 | 12 | 300 | 1.21e-86 | 261 |
| MsG0880047495.01.T01 | AT4G13090 | 45.936 | 283 | 142 | 6 | 8 | 283 | 10 | 288 | 1.44e-78 | 240 |
| MsG0880047495.01.T01 | AT2G14620 | 50.649 | 231 | 108 | 4 | 57 | 284 | 18 | 245 | 7.82e-78 | 237 |
| MsG0880047495.01.T01 | AT4G13080 | 48.062 | 258 | 129 | 5 | 26 | 283 | 39 | 291 | 4.95e-76 | 234 |
| MsG0880047495.01.T01 | AT2G14620 | 51.351 | 222 | 102 | 4 | 66 | 284 | 16 | 234 | 6.51e-76 | 231 |
| MsG0880047495.01.T01 | AT3G25050 | 45.211 | 261 | 134 | 6 | 26 | 283 | 35 | 289 | 6.18e-74 | 228 |
| MsG0880047495.01.T01 | AT2G36870 | 41.935 | 279 | 143 | 7 | 13 | 283 | 30 | 297 | 5.61e-64 | 203 |
| MsG0880047495.01.T01 | AT3G44990 | 42.481 | 266 | 134 | 7 | 26 | 283 | 39 | 293 | 3.39e-61 | 196 |
| MsG0880047495.01.T01 | AT1G32170 | 35.117 | 299 | 169 | 10 | 3 | 284 | 4 | 294 | 4.08e-52 | 174 |
| MsG0880047495.01.T01 | AT1G10550 | 36.398 | 261 | 149 | 7 | 37 | 283 | 52 | 309 | 2.18e-51 | 171 |
| MsG0880047495.01.T01 | AT1G14720 | 35.581 | 267 | 160 | 7 | 25 | 284 | 30 | 291 | 3.85e-49 | 166 |
| MsG0880047495.01.T01 | AT2G01850 | 32.203 | 295 | 185 | 7 | 1 | 284 | 1 | 291 | 2.34e-47 | 161 |
| MsG0880047495.01.T01 | AT3G48580 | 36.653 | 251 | 141 | 7 | 33 | 283 | 41 | 273 | 1.63e-44 | 152 |
| MsG0880047495.01.T01 | AT4G18990 | 34.164 | 281 | 156 | 9 | 26 | 284 | 40 | 313 | 1.14e-43 | 152 |
| MsG0880047495.01.T01 | AT3G48580 | 35.526 | 228 | 130 | 6 | 56 | 283 | 4 | 214 | 1.11e-38 | 135 |
| MsG0880047495.01.T01 | AT4G18990 | 34.722 | 216 | 113 | 8 | 91 | 284 | 30 | 239 | 4.68e-35 | 128 |
| MsG0880047495.01.T01 | AT2G36870 | 43.709 | 151 | 75 | 3 | 13 | 155 | 30 | 178 | 6.54e-33 | 119 |
| MsG0880047495.01.T01 | AT3G48580 | 31.474 | 251 | 134 | 8 | 33 | 283 | 41 | 253 | 1.15e-27 | 107 |
Find 63 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGGCGTTGTTGAGTATTT+TGG | 0.226569 | 8:-87138215 | None:intergenic |
| CTGTAGATGGAACTCCAATT+AGG | 0.235182 | 8:+87138812 | MsG0880047495.01.T01:CDS |
| CTCCATTTACCGCTTCTTAC+AGG | 0.238448 | 8:+87138965 | MsG0880047495.01.T01:CDS |
| ATGTTCTTGAATTCCCTAAT+TGG | 0.259290 | 8:-87138826 | None:intergenic |
| TGAGAATCTATTCTAGTCTT+TGG | 0.265403 | 8:+87138887 | MsG0880047495.01.T01:CDS |
| GAGATAGACTTTGAATTCTT+AGG | 0.299139 | 8:+87138520 | MsG0880047495.01.T01:CDS |
| TGTTTCTTATTCCTCTGCTT+TGG | 0.321072 | 8:+87138125 | MsG0880047495.01.T01:CDS |
| TTCCTGTAAGAAGCGGTAAA+TGG | 0.328030 | 8:-87138967 | None:intergenic |
| GTCTTTGGAATGCTGATGAT+TGG | 0.342081 | 8:+87138902 | MsG0880047495.01.T01:CDS |
| TGTAGATGGAACTCCAATTA+GGG | 0.398243 | 8:+87138813 | MsG0880047495.01.T01:CDS |
| CTTGACAAAGCCTCTGGCTC+TGG | 0.398860 | 8:+87138253 | MsG0880047495.01.T01:CDS |
| GTTCCATGTACAAGCATTGT+TGG | 0.399945 | 8:-87138998 | None:intergenic |
| ATCTAGTGAGTGCATTCAAC+TGG | 0.406472 | 8:-87139180 | None:intergenic |
| ATGCAGATTAAGCTTGTTCC+AGG | 0.407701 | 8:+87138316 | MsG0880047495.01.T01:CDS |
| GCTCTCTGTTACCTTTGCCT+TGG | 0.439694 | 8:-87138588 | None:intergenic |
| AGACTAGAATAGATTCTCAT+TGG | 0.441045 | 8:-87138883 | None:intergenic |
| AGAAGCGGTAAATGGAGCTT+TGG | 0.460589 | 8:-87138959 | None:intergenic |
| TCACAACAGCTTGACTCAAC+AGG | 0.461793 | 8:+87139075 | MsG0880047495.01.T01:CDS |
| TAGAATAGATTCTCATTGGT+TGG | 0.468992 | 8:-87138879 | None:intergenic |
| TCTTTGGAATGCTGATGATT+GGG | 0.473720 | 8:+87138903 | MsG0880047495.01.T01:CDS |
| CTTGTTCCAGGCAACTCTGC+TGG | 0.477831 | 8:+87138328 | MsG0880047495.01.T01:CDS |
| CTGTCTCTTGACAAAGCCTC+TGG | 0.485728 | 8:+87138247 | MsG0880047495.01.T01:CDS |
| CCCATGTAATCTGAAAATCT+TGG | 0.491907 | 8:-87138180 | None:intergenic |
| ACATTGGTGTGAAGGATGTA+AGG | 0.496043 | 8:-87138559 | None:intergenic |
| CAGGTCTGCAGAGGCTGAGG+TGG | 0.507933 | 8:+87139094 | MsG0880047495.01.T01:CDS |
| GTGGACTTGTTAAGACAGAT+TGG | 0.508499 | 8:+87138935 | MsG0880047495.01.T01:CDS |
| CAACAGGTCTGCAGAGGCTG+AGG | 0.508970 | 8:+87139091 | MsG0880047495.01.T01:CDS |
| TTTCAGATTACATGGGGAGA+TGG | 0.522270 | 8:+87138187 | MsG0880047495.01.T01:CDS |
| ACAGTGCCAGCAGAGTTGCC+TGG | 0.527429 | 8:-87138334 | None:intergenic |
| AAGAGACAGAGTAAGAAGAT+TGG | 0.527904 | 8:-87138233 | None:intergenic |
| TCACTTCAGCTATCATCAAA+AGG | 0.530973 | 8:+87138484 | MsG0880047495.01.T01:intron |
| ATGCCAACAATGCTTGTACA+TGG | 0.537452 | 8:+87138995 | MsG0880047495.01.T01:CDS |
| TTCACACATACTCAATTCTG+TGG | 0.546538 | 8:+87138647 | MsG0880047495.01.T01:CDS |
| CATTCAACTGGAAGGCCTTG+TGG | 0.552461 | 8:-87139168 | None:intergenic |
| CAAAACCCTTTGCTTCCTCA+TGG | 0.555520 | 8:+87139049 | MsG0880047495.01.T01:CDS |
| CACACCAATGTGTTTAGCCA+AGG | 0.563595 | 8:+87138571 | MsG0880047495.01.T01:CDS |
| ATCATCAAAAGGAGGTGCAT+GGG | 0.570048 | 8:+87138495 | MsG0880047495.01.T01:CDS |
| TGATAGCCATGAGGAAGCAA+AGG | 0.571137 | 8:-87139055 | None:intergenic |
| GATAGCCATGAGGAAGCAAA+GGG | 0.575488 | 8:-87139054 | None:intergenic |
| TATCATCAAAAGGAGGTGCA+TGG | 0.582020 | 8:+87138494 | MsG0880047495.01.T01:CDS |
| CTGCAGTTGGGTCAAACCAA+AGG | 0.586715 | 8:-87138621 | None:intergenic |
| AATTAGGGAATTCAAGAACA+TGG | 0.587989 | 8:+87138828 | MsG0880047495.01.T01:CDS |
| TTTGCCTTGGCTAAACACAT+TGG | 0.588875 | 8:-87138575 | None:intergenic |
| AGGTCTGCAGAGGCTGAGGT+GGG | 0.596207 | 8:+87139095 | MsG0880047495.01.T01:CDS |
| GATGATTGGGCTACAAGAGG+TGG | 0.596350 | 8:+87138916 | MsG0880047495.01.T01:CDS |
| ATTAATGTAGCATGTGCTGC+TGG | 0.599927 | 8:+87138148 | MsG0880047495.01.T01:CDS |
| GGATTGAAAGCCAGAGCCAG+AGG | 0.605791 | 8:-87138263 | None:intergenic |
| ATGTGTGAAAATCTGCAGTT+GGG | 0.606525 | 8:-87138633 | None:intergenic |
| GGCTAAACACATTGGTGTGA+AGG | 0.607001 | 8:-87138567 | None:intergenic |
| AATGCTTGTACATGGAACAA+TGG | 0.607069 | 8:+87139003 | MsG0880047495.01.T01:CDS |
| AGTACTTACACAATACGTTG+TGG | 0.616337 | 8:-87138673 | None:intergenic |
| TATGTGTGAAAATCTGCAGT+TGG | 0.616665 | 8:-87138634 | None:intergenic |
| TTGACTCAACAGGTCTGCAG+AGG | 0.621733 | 8:+87139085 | MsG0880047495.01.T01:CDS |
| TTCAAGAACATGGAATCAAG+TGG | 0.625602 | 8:+87138838 | MsG0880047495.01.T01:CDS |
| AGTGAGTGCATTCAACTGGA+AGG | 0.634779 | 8:-87139176 | None:intergenic |
| ATTGAAATTCCTGTAAGAAG+CGG | 0.652103 | 8:-87138974 | None:intergenic |
| GAATTCTTAGGAAATTTGAG+TGG | 0.658307 | 8:+87138532 | MsG0880047495.01.T01:CDS |
| AATGTGTTTAGCCAAGGCAA+AGG | 0.659745 | 8:+87138577 | MsG0880047495.01.T01:CDS |
| CTTCAGCTATCATCAAAAGG+AGG | 0.677139 | 8:+87138487 | MsG0880047495.01.T01:intron |
| GCTGATGATTGGGCTACAAG+AGG | 0.684485 | 8:+87138913 | MsG0880047495.01.T01:CDS |
| GCTACATTAATCCAAAGCAG+AGG | 0.687490 | 8:-87138136 | None:intergenic |
| AAGCTGTTGTGATAGCCATG+AGG | 0.744355 | 8:-87139064 | None:intergenic |
| GATAAGAAGAGATTTCCACA+AGG | 0.790444 | 8:+87139153 | MsG0880047495.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAAACATTACTTGTGT+TGG | + | Chr8:87138744-87138763 | MsG0880047495.01.T01:intron | 20.0% |
| !! | TCCAAAAATGAATACTTATT+TGG | + | Chr8:87138283-87138302 | MsG0880047495.01.T01:CDS | 20.0% |
| !! | GCCAAATAAGTATTCATTTT+TGG | - | Chr8:87138287-87138306 | None:intergenic | 25.0% |
| AATTAGGGAATTCAAGAACA+TGG | + | Chr8:87138828-87138847 | MsG0880047495.01.T01:CDS | 30.0% | |
| AGACTAGAATAGATTCTCAT+TGG | - | Chr8:87138886-87138905 | None:intergenic | 30.0% | |
| ATGTTCTTGAATTCCCTAAT+TGG | - | Chr8:87138829-87138848 | None:intergenic | 30.0% | |
| ATTGAAATTCCTGTAAGAAG+CGG | - | Chr8:87138977-87138996 | None:intergenic | 30.0% | |
| GAATTCTTAGGAAATTTGAG+TGG | + | Chr8:87138532-87138551 | MsG0880047495.01.T01:CDS | 30.0% | |
| GAGATAGACTTTGAATTCTT+AGG | + | Chr8:87138520-87138539 | MsG0880047495.01.T01:CDS | 30.0% | |
| TAGAATAGATTCTCATTGGT+TGG | - | Chr8:87138882-87138901 | None:intergenic | 30.0% | |
| ! | ACCAAGATTTTCAGATTACA+TGG | + | Chr8:87138179-87138198 | MsG0880047495.01.T01:CDS | 30.0% |
| ! | CAAGATTTTCAGATTACATG+GGG | + | Chr8:87138181-87138200 | MsG0880047495.01.T01:CDS | 30.0% |
| ! | CCAAGATTTTCAGATTACAT+GGG | + | Chr8:87138180-87138199 | MsG0880047495.01.T01:CDS | 30.0% |
| ! | TGAGAATCTATTCTAGTCTT+TGG | + | Chr8:87138887-87138906 | MsG0880047495.01.T01:CDS | 30.0% |
| !! | TTTTTCAGTTTCTCTGTAGA+TGG | + | Chr8:87138799-87138818 | MsG0880047495.01.T01:intron | 30.0% |
| !!! | ATTCTCATTGGTTGGTTTTT+GGG | - | Chr8:87138874-87138893 | None:intergenic | 30.0% |
| AAGAGACAGAGTAAGAAGAT+TGG | - | Chr8:87138236-87138255 | None:intergenic | 35.0% | |
| AATGCTTGTACATGGAACAA+TGG | + | Chr8:87139003-87139022 | MsG0880047495.01.T01:CDS | 35.0% | |
| AGTACTTACACAATACGTTG+TGG | - | Chr8:87138676-87138695 | None:intergenic | 35.0% | |
| ATGTGTGAAAATCTGCAGTT+GGG | - | Chr8:87138636-87138655 | None:intergenic | 35.0% | |
| CCCATGTAATCTGAAAATCT+TGG | - | Chr8:87138183-87138202 | None:intergenic | 35.0% | |
| GATAAGAAGAGATTTCCACA+AGG | + | Chr8:87139153-87139172 | MsG0880047495.01.T01:CDS | 35.0% | |
| TATGTGTGAAAATCTGCAGT+TGG | - | Chr8:87138637-87138656 | None:intergenic | 35.0% | |
| TCACTTCAGCTATCATCAAA+AGG | + | Chr8:87138484-87138503 | MsG0880047495.01.T01:intron | 35.0% | |
| TGTAGATGGAACTCCAATTA+GGG | + | Chr8:87138813-87138832 | MsG0880047495.01.T01:CDS | 35.0% | |
| TGTTTCTTATTCCTCTGCTT+TGG | + | Chr8:87138125-87138144 | MsG0880047495.01.T01:CDS | 35.0% | |
| TTCAAGAACATGGAATCAAG+TGG | + | Chr8:87138838-87138857 | MsG0880047495.01.T01:CDS | 35.0% | |
| TTCACACATACTCAATTCTG+TGG | + | Chr8:87138647-87138666 | MsG0880047495.01.T01:CDS | 35.0% | |
| ! | ATTGGCGTTGTTGAGTATTT+TGG | - | Chr8:87138218-87138237 | None:intergenic | 35.0% |
| ! | GAGAGCAACAATTTTACCTT+TGG | + | Chr8:87138605-87138624 | MsG0880047495.01.T01:CDS | 35.0% |
| !! | TCTTTGGAATGCTGATGATT+GGG | + | Chr8:87138903-87138922 | MsG0880047495.01.T01:CDS | 35.0% |
| !!! | GATTCTCATTGGTTGGTTTT+TGG | - | Chr8:87138875-87138894 | None:intergenic | 35.0% |
| !!! | TTCTCATTGGTTGGTTTTTG+GGG | - | Chr8:87138873-87138892 | None:intergenic | 35.0% |
| AATGTGTTTAGCCAAGGCAA+AGG | + | Chr8:87138577-87138596 | MsG0880047495.01.T01:CDS | 40.0% | |
| ACATTGGTGTGAAGGATGTA+AGG | - | Chr8:87138562-87138581 | None:intergenic | 40.0% | |
| ATGCCAACAATGCTTGTACA+TGG | + | Chr8:87138995-87139014 | MsG0880047495.01.T01:CDS | 40.0% | |
| ATTAATGTAGCATGTGCTGC+TGG | + | Chr8:87138148-87138167 | MsG0880047495.01.T01:CDS | 40.0% | |
| CTGTAGATGGAACTCCAATT+AGG | + | Chr8:87138812-87138831 | MsG0880047495.01.T01:CDS | 40.0% | |
| CTTCAGCTATCATCAAAAGG+AGG | + | Chr8:87138487-87138506 | MsG0880047495.01.T01:intron | 40.0% | |
| GCTACATTAATCCAAAGCAG+AGG | - | Chr8:87138139-87138158 | None:intergenic | 40.0% | |
| TTCCTGTAAGAAGCGGTAAA+TGG | - | Chr8:87138970-87138989 | None:intergenic | 40.0% | |
| TTTCAGATTACATGGGGAGA+TGG | + | Chr8:87138187-87138206 | MsG0880047495.01.T01:CDS | 40.0% | |
| TTTGCCTTGGCTAAACACAT+TGG | - | Chr8:87138578-87138597 | None:intergenic | 40.0% | |
| ! | ATCATCAAAAGGAGGTGCAT+GGG | + | Chr8:87138495-87138514 | MsG0880047495.01.T01:CDS | 40.0% |
| ! | ATGCAGATTAAGCTTGTTCC+AGG | + | Chr8:87138316-87138335 | MsG0880047495.01.T01:CDS | 40.0% |
| ! | GTTCCATGTACAAGCATTGT+TGG | - | Chr8:87139001-87139020 | None:intergenic | 40.0% |
| ! | TATCATCAAAAGGAGGTGCA+TGG | + | Chr8:87138494-87138513 | MsG0880047495.01.T01:CDS | 40.0% |
| !! | GTCTTTGGAATGCTGATGAT+TGG | + | Chr8:87138902-87138921 | MsG0880047495.01.T01:CDS | 40.0% |
| !! | GTGGACTTGTTAAGACAGAT+TGG | + | Chr8:87138935-87138954 | MsG0880047495.01.T01:CDS | 40.0% |
| !!! | ATTGGTTGGTTTTTGGGGAA+TGG | - | Chr8:87138868-87138887 | None:intergenic | 40.0% |
| AAGCTGTTGTGATAGCCATG+AGG | - | Chr8:87139067-87139086 | None:intergenic | 45.0% | |
| AGAAGCGGTAAATGGAGCTT+TGG | - | Chr8:87138962-87138981 | None:intergenic | 45.0% | |
| AGTGAGTGCATTCAACTGGA+AGG | - | Chr8:87139179-87139198 | None:intergenic | 45.0% | |
| CAAAACCCTTTGCTTCCTCA+TGG | + | Chr8:87139049-87139068 | MsG0880047495.01.T01:CDS | 45.0% | |
| CACACCAATGTGTTTAGCCA+AGG | + | Chr8:87138571-87138590 | MsG0880047495.01.T01:CDS | 45.0% | |
| CTCCATTTACCGCTTCTTAC+AGG | + | Chr8:87138965-87138984 | MsG0880047495.01.T01:CDS | 45.0% | |
| GATAGCCATGAGGAAGCAAA+GGG | - | Chr8:87139057-87139076 | None:intergenic | 45.0% | |
| GGCTAAACACATTGGTGTGA+AGG | - | Chr8:87138570-87138589 | None:intergenic | 45.0% | |
| TCACAACAGCTTGACTCAAC+AGG | + | Chr8:87139075-87139094 | MsG0880047495.01.T01:CDS | 45.0% | |
| TGATAGCCATGAGGAAGCAA+AGG | - | Chr8:87139058-87139077 | None:intergenic | 45.0% | |
| CATTCAACTGGAAGGCCTTG+TGG | - | Chr8:87139171-87139190 | None:intergenic | 50.0% | |
| CTGCAGTTGGGTCAAACCAA+AGG | - | Chr8:87138624-87138643 | None:intergenic | 50.0% | |
| CTGTCTCTTGACAAAGCCTC+TGG | + | Chr8:87138247-87138266 | MsG0880047495.01.T01:CDS | 50.0% | |
| GATGATTGGGCTACAAGAGG+TGG | + | Chr8:87138916-87138935 | MsG0880047495.01.T01:CDS | 50.0% | |
| GCTCTCTGTTACCTTTGCCT+TGG | - | Chr8:87138591-87138610 | None:intergenic | 50.0% | |
| GCTGATGATTGGGCTACAAG+AGG | + | Chr8:87138913-87138932 | MsG0880047495.01.T01:CDS | 50.0% | |
| TTGACTCAACAGGTCTGCAG+AGG | + | Chr8:87139085-87139104 | MsG0880047495.01.T01:CDS | 50.0% | |
| CTTGTTCCAGGCAACTCTGC+TGG | + | Chr8:87138328-87138347 | MsG0880047495.01.T01:CDS | 55.0% | |
| GGATTGAAAGCCAGAGCCAG+AGG | - | Chr8:87138266-87138285 | None:intergenic | 55.0% | |
| ! | CTTGACAAAGCCTCTGGCTC+TGG | + | Chr8:87138253-87138272 | MsG0880047495.01.T01:CDS | 55.0% |
| ACAGTGCCAGCAGAGTTGCC+TGG | - | Chr8:87138337-87138356 | None:intergenic | 60.0% | |
| AGGTCTGCAGAGGCTGAGGT+GGG | + | Chr8:87139095-87139114 | MsG0880047495.01.T01:CDS | 60.0% | |
| CAACAGGTCTGCAGAGGCTG+AGG | + | Chr8:87139091-87139110 | MsG0880047495.01.T01:CDS | 60.0% | |
| CAGGTCTGCAGAGGCTGAGG+TGG | + | Chr8:87139094-87139113 | MsG0880047495.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 87138100 | 87139200 | 87138100 | ID=MsG0880047495.01;Name=MsG0880047495.01 |
| Chr8 | mRNA | 87138100 | 87139200 | 87138100 | ID=MsG0880047495.01.T01;Parent=MsG0880047495.01;Name=MsG0880047495.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|3|0|285 |
| Chr8 | exon | 87138100 | 87138369 | 87138100 | ID=MsG0880047495.01.T01:exon:17997;Parent=MsG0880047495.01.T01 |
| Chr8 | exon | 87138493 | 87138686 | 87138493 | ID=MsG0880047495.01.T01:exon:17998;Parent=MsG0880047495.01.T01 |
| Chr8 | exon | 87138807 | 87139200 | 87138807 | ID=MsG0880047495.01.T01:exon:17999;Parent=MsG0880047495.01.T01 |
| Chr8 | CDS | 87138100 | 87138369 | 87138100 | ID=MsG0880047495.01.T01:cds;Parent=MsG0880047495.01.T01 |
| Chr8 | CDS | 87138493 | 87138686 | 87138493 | ID=MsG0880047495.01.T01:cds;Parent=MsG0880047495.01.T01 |
| Chr8 | CDS | 87138807 | 87139200 | 87138807 | ID=MsG0880047495.01.T01:cds;Parent=MsG0880047495.01.T01 |
| Gene Sequence |
| Protein sequence |
