Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0001360 | Msa0200090 | 0.812238 | 4.688403e-51 | -8.615850e-47 |
| Msa0001360 | Msa0629030 | 0.822000 | 2.992754e-53 | -8.615850e-47 |
| Msa0001360 | Msa0666580 | 0.816488 | 5.388013e-52 | -8.615850e-47 |
| Msa0001360 | Msa1383770 | 0.988989 | 3.103712e-176 | -8.615850e-47 |
| Msa0001360 | Msa1442910 | 0.989277 | 1.953685e-177 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0001360 | MtrunA17_Chr3g0142831 | 95.455 | 110 | 5 | 0 | 1 | 110 | 1 | 110 | 1.69e-73 | 213 |
| Msa0001360 | MtrunA17_Chr1g0156781 | 52.294 | 109 | 45 | 1 | 1 | 102 | 1 | 109 | 5.77e-33 | 111 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0001360 | AT1G75810.1 | 56.800 | 125 | 36 | 3 | 1 | 110 | 4 | 125 | 4.61e-41 | 132 |
Find 35 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGAAAGCGGCGTTGGGAAT+AGG | 0.382735 | 1_1:+3053233 | None:intergenic |
| CGTCCGATCACCGGCTTCTC+AGG | 0.402331 | 1_1:-3053285 | Msa0001360:CDS |
| CGTGGTGGGCTTTATCCTAA+TGG | 0.420862 | 1_1:-3053411 | Msa0001360:CDS |
| ATTGTAAGGGAGGTATTTGT+TGG | 0.421438 | 1_1:-3053319 | Msa0001360:CDS |
| CCCACGTCAGCGTTGCCATT+AGG | 0.438021 | 1_1:+3053396 | None:intergenic |
| TCATCCACCGTTGAATTGTA+AGG | 0.438749 | 1_1:-3053333 | Msa0001360:CDS |
| CTCTGTGCTCGTACCTTCAC+CGG | 0.467220 | 1_1:-3053461 | Msa0001360:CDS |
| CTATCATTAGAAAGCGGCGT+TGG | 0.486546 | 1_1:+3053226 | None:intergenic |
| CGGTGTCGCCGTCCGATCAC+CGG | 0.487196 | 1_1:-3053294 | Msa0001360:CDS |
| TATCATTAGAAAGCGGCGTT+GGG | 0.494168 | 1_1:+3053227 | None:intergenic |
| CAGCCACGTAGATTGTTGTA+AGG | 0.502912 | 1_1:+3053513 | None:intergenic |
| TACAATTCAACGGTGGATGA+TGG | 0.506956 | 1_1:+3053336 | None:intergenic |
| CTGAAACGGACCTGAGAAGC+CGG | 0.530738 | 1_1:+3053275 | None:intergenic |
| TCCTAATGGCAACGCTGACG+TGG | 0.535183 | 1_1:-3053397 | Msa0001360:CDS |
| AAAGCCGTAGAGCTTAATAC+AGG | 0.546620 | 1_1:+3053484 | None:intergenic |
| GGAATAGGAAATATCTGTTG+TGG | 0.547341 | 1_1:+3053248 | None:intergenic |
| ATCGCCTGTATTAAGCTCTA+CGG | 0.548367 | 1_1:-3053488 | Msa0001360:CDS |
| TCTGTTGTGGAATTCTGAAA+CGG | 0.550614 | 1_1:+3053261 | None:intergenic |
| ATGATATCAACCACCGTGGT+GGG | 0.553215 | 1_1:-3053425 | Msa0001360:CDS |
| GGACCTGAGAAGCCGGTGAT+CGG | 0.556230 | 1_1:+3053282 | None:intergenic |
| CTGAGAAGCCGGTGATCGGA+CGG | 0.557696 | 1_1:+3053286 | None:intergenic |
| AAGGGAGGTATTTGTTGGCA+CGG | 0.595376 | 1_1:-3053314 | Msa0001360:CDS |
| CATCCACCGTTGAATTGTAA+GGG | 0.599993 | 1_1:-3053332 | Msa0001360:CDS |
| TCAACGGTGGATGATGGTAG+CGG | 0.620485 | 1_1:+3053342 | None:intergenic |
| CCTAATGGCAACGCTGACGT+GGG | 0.634171 | 1_1:-3053396 | Msa0001360:CDS |
| ATACCTCCCTTACAATTCAA+CGG | 0.637027 | 1_1:+3053326 | None:intergenic |
| ATCTCACTATCATTAGAAAG+CGG | 0.641809 | 1_1:+3053220 | None:intergenic |
| CATGATATCAACCACCGTGG+TGG | 0.657627 | 1_1:-3053426 | Msa0001360:CDS |
| CCACCGTTGAATTGTAAGGG+AGG | 0.677032 | 1_1:-3053329 | Msa0001360:CDS |
| GTAGCGGTTGATAGCATACG+TGG | 0.718415 | 1_1:+3053358 | None:intergenic |
| CAACCTTACAACAATCTACG+TGG | 0.719583 | 1_1:-3053516 | Msa0001360:CDS |
| CATTAGGATAAAGCCCACCA+CGG | 0.727327 | 1_1:+3053412 | None:intergenic |
| CCTCCCTTACAATTCAACGG+TGG | 0.751522 | 1_1:+3053329 | None:intergenic |
| TAGGATAAAGCCCACCACGG+TGG | 0.764291 | 1_1:+3053415 | None:intergenic |
| TGTCATGATATCAACCACCG+TGG | 0.843191 | 1_1:-3053429 | Msa0001360:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TATCATGACAACAAAAACGC+CGG | + | chr1_1:3053335-3053354 | None:intergenic | 35.0% | |
| ATACCTCCCTTACAATTCAA+CGG | + | chr1_1:3053451-3053470 | None:intergenic | 35.0% | |
| !! | ATTGTAAGGGAGGTATTTGT+TGG | - | chr1_1:3053455-3053474 | Msa0001360:CDS | 35.0% |
| ! | TCTGTTGTGGAATTCTGAAA+CGG | + | chr1_1:3053516-3053535 | None:intergenic | 35.0% |
| GGAATAGGAAATATCTGTTG+TGG | + | chr1_1:3053529-3053548 | None:intergenic | 35.0% | |
| CAACCTTACAACAATCTACG+TGG | - | chr1_1:3053258-3053277 | Msa0001360:CDS | 40.0% | |
| ATCGCCTGTATTAAGCTCTA+CGG | - | chr1_1:3053286-3053305 | Msa0001360:CDS | 40.0% | |
| AAAGCCGTAGAGCTTAATAC+AGG | + | chr1_1:3053293-3053312 | None:intergenic | 40.0% | |
| TACAATTCAACGGTGGATGA+TGG | + | chr1_1:3053441-3053460 | None:intergenic | 40.0% | |
| TCATCCACCGTTGAATTGTA+AGG | - | chr1_1:3053441-3053460 | Msa0001360:CDS | 40.0% | |
| CATCCACCGTTGAATTGTAA+GGG | - | chr1_1:3053442-3053461 | Msa0001360:CDS | 40.0% | |
| CAGCCACGTAGATTGTTGTA+AGG | + | chr1_1:3053264-3053283 | None:intergenic | 45.0% | |
| TGTCATGATATCAACCACCG+TGG | - | chr1_1:3053345-3053364 | Msa0001360:CDS | 45.0% | |
| ATGATATCAACCACCGTGGT+GGG | - | chr1_1:3053349-3053368 | Msa0001360:CDS | 45.0% | |
| CATTAGGATAAAGCCCACCA+CGG | + | chr1_1:3053365-3053384 | None:intergenic | 45.0% | |
| !! | AAGGGAGGTATTTGTTGGCA+CGG | - | chr1_1:3053460-3053479 | Msa0001360:CDS | 45.0% |
| GACAACAAAAACGCCGGTGA+AGG | + | chr1_1:3053329-3053348 | None:intergenic | 50.0% | |
| CATGATATCAACCACCGTGG+TGG | - | chr1_1:3053348-3053367 | Msa0001360:CDS | 50.0% | |
| !! | CGTGGTGGGCTTTATCCTAA+TGG | - | chr1_1:3053363-3053382 | Msa0001360:CDS | 50.0% |
| !! | GTAGCGGTTGATAGCATACG+TGG | + | chr1_1:3053419-3053438 | None:intergenic | 50.0% |
| ! | TCAACGGTGGATGATGGTAG+CGG | + | chr1_1:3053435-3053454 | None:intergenic | 50.0% |
| CCTCCCTTACAATTCAACGG+TGG | + | chr1_1:3053448-3053467 | None:intergenic | 50.0% | |
| CCACCGTTGAATTGTAAGGG+AGG | - | chr1_1:3053445-3053464 | Msa0001360:CDS | 50.0% | |
| ! | TAGAAAGCGGCGTTGGGAAT+AGG | + | chr1_1:3053544-3053563 | None:intergenic | 50.0% |
| CTCTGTGCTCGTACCTTCAC+CGG | - | chr1_1:3053313-3053332 | Msa0001360:CDS | 55.0% | |
| TAGGATAAAGCCCACCACGG+TGG | + | chr1_1:3053362-3053381 | None:intergenic | 55.0% | |
| TCCTAATGGCAACGCTGACG+TGG | - | chr1_1:3053377-3053396 | Msa0001360:CDS | 55.0% | |
| CCTAATGGCAACGCTGACGT+GGG | - | chr1_1:3053378-3053397 | Msa0001360:CDS | 55.0% | |
| CTGAAACGGACCTGAGAAGC+CGG | + | chr1_1:3053502-3053521 | None:intergenic | 55.0% | |
| CCCACGTCAGCGTTGCCATT+AGG | + | chr1_1:3053381-3053400 | None:intergenic | 60.0% | |
| CTGAGAAGCCGGTGATCGGA+CGG | + | chr1_1:3053491-3053510 | None:intergenic | 60.0% | |
| GGACCTGAGAAGCCGGTGAT+CGG | + | chr1_1:3053495-3053514 | None:intergenic | 60.0% | |
| !! | CGTCCGATCACCGGCTTCTC+AGG | - | chr1_1:3053489-3053508 | Msa0001360:CDS | 65.0% |
| !! | CGGTGTCGCCGTCCGATCAC+CGG | - | chr1_1:3053480-3053499 | Msa0001360:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_1 | gene | 3053232 | 3053564 | 3053232 | ID=Msa0001360;Name=Msa0001360 |
| chr1_1 | mRNA | 3053232 | 3053564 | 3053232 | ID=Msa0001360-mRNA-1;Parent=Msa0001360;Name=Msa0001360-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|110 |
| chr1_1 | exon | 3053232 | 3053564 | 3053232 | ID=Msa0001360-mRNA-1:exon:701;Parent=Msa0001360-mRNA-1 |
| chr1_1 | CDS | 3053232 | 3053564 | 3053232 | ID=Msa0001360-mRNA-1:cds;Parent=Msa0001360-mRNA-1 |
| Gene Sequence |
| Protein sequence |
