Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1442910 | A0A072VDK4 | 95.455 | 110 | 5 | 0 | 1 | 110 | 1 | 110 | 8.76e-70 | 213 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0001360 | Msa1442910 | 0.989277 | 1.953685e-177 | -8.615850e-47 |
| Msa1383770 | Msa1442910 | 0.999537 | 1.634369e-320 | -8.615850e-47 |
| Msa0629030 | Msa1442910 | 0.819786 | 9.674120e-53 | -8.615850e-47 |
| Msa0666580 | Msa1442910 | 0.812000 | 5.283504e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1442910 | MtrunA17_Chr3g0142831 | 95.455 | 110 | 5 | 0 | 1 | 110 | 1 | 110 | 1.69e-73 | 213 |
| Msa1442910 | MtrunA17_Chr1g0156781 | 52.294 | 109 | 45 | 1 | 1 | 102 | 1 | 109 | 5.77e-33 | 111 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1442910 | AT1G75810.1 | 56.800 | 125 | 36 | 3 | 1 | 110 | 4 | 125 | 4.61e-41 | 132 |
Find 35 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGAAAGCGGCGTTGGGAAT+AGG | 0.382735 | tig0030223:+27620 | None:intergenic |
| CGTCCGATCACCGGCTTCTC+AGG | 0.402331 | tig0030223:-27672 | Msa1442910:CDS |
| CGTGGTGGGCTTTATCCTAA+TGG | 0.420862 | tig0030223:-27798 | Msa1442910:CDS |
| ATTGTAAGGGAGGTATTTGT+TGG | 0.421438 | tig0030223:-27706 | Msa1442910:CDS |
| CCCACGTCAGCGTTGCCATT+AGG | 0.438021 | tig0030223:+27783 | None:intergenic |
| TCATCCACCGTTGAATTGTA+AGG | 0.438749 | tig0030223:-27720 | Msa1442910:CDS |
| CTCTGTGCTCGTACCTTCAC+CGG | 0.467220 | tig0030223:-27848 | Msa1442910:CDS |
| CTATCATTAGAAAGCGGCGT+TGG | 0.486546 | tig0030223:+27613 | None:intergenic |
| CGGTGTCGCCGTCCGATCAC+CGG | 0.487196 | tig0030223:-27681 | Msa1442910:CDS |
| TATCATTAGAAAGCGGCGTT+GGG | 0.494168 | tig0030223:+27614 | None:intergenic |
| CAGCCACGTAGATTGTTGTA+AGG | 0.502912 | tig0030223:+27900 | None:intergenic |
| TACAATTCAACGGTGGATGA+TGG | 0.506956 | tig0030223:+27723 | None:intergenic |
| CTGAAACGGACCTGAGAAGC+CGG | 0.530738 | tig0030223:+27662 | None:intergenic |
| TCCTAATGGCAACGCTGACG+TGG | 0.535183 | tig0030223:-27784 | Msa1442910:CDS |
| AAAGCCGTAGAGCTTAATAC+AGG | 0.546620 | tig0030223:+27871 | None:intergenic |
| GGAATAGGAAATATCTGTTG+TGG | 0.547341 | tig0030223:+27635 | None:intergenic |
| ATCGCCTGTATTAAGCTCTA+CGG | 0.548367 | tig0030223:-27875 | Msa1442910:CDS |
| TCTGTTGTGGAATTCTGAAA+CGG | 0.550614 | tig0030223:+27648 | None:intergenic |
| ATGATATCAACCACCGTGGT+GGG | 0.553215 | tig0030223:-27812 | Msa1442910:CDS |
| GGACCTGAGAAGCCGGTGAT+CGG | 0.556230 | tig0030223:+27669 | None:intergenic |
| CTGAGAAGCCGGTGATCGGA+CGG | 0.557696 | tig0030223:+27673 | None:intergenic |
| AAGGGAGGTATTTGTTGGCA+CGG | 0.595376 | tig0030223:-27701 | Msa1442910:CDS |
| CATCCACCGTTGAATTGTAA+GGG | 0.599993 | tig0030223:-27719 | Msa1442910:CDS |
| TCAACGGTGGATGATGGTAG+CGG | 0.620485 | tig0030223:+27729 | None:intergenic |
| CCTAATGGCAACGCTGACGT+GGG | 0.634171 | tig0030223:-27783 | Msa1442910:CDS |
| ATACCTCCCTTACAATTCAA+CGG | 0.637027 | tig0030223:+27713 | None:intergenic |
| ATCTCACTATCATTAGAAAG+CGG | 0.641809 | tig0030223:+27607 | None:intergenic |
| CATGATATCAACCACCGTGG+TGG | 0.657627 | tig0030223:-27813 | Msa1442910:CDS |
| CCACCGTTGAATTGTAAGGG+AGG | 0.677032 | tig0030223:-27716 | Msa1442910:CDS |
| GTAGCGGTTGATAGCATACG+TGG | 0.718415 | tig0030223:+27745 | None:intergenic |
| CAACCTTACAACAATCTACG+TGG | 0.719583 | tig0030223:-27903 | Msa1442910:CDS |
| CATTAGGATAAAGCCCACCA+CGG | 0.727327 | tig0030223:+27799 | None:intergenic |
| CCTCCCTTACAATTCAACGG+TGG | 0.751522 | tig0030223:+27716 | None:intergenic |
| TAGGATAAAGCCCACCACGG+TGG | 0.764291 | tig0030223:+27802 | None:intergenic |
| TGTCATGATATCAACCACCG+TGG | 0.843191 | tig0030223:-27816 | Msa1442910:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TATCATGACAACAAAAACGC+CGG | + | tig0030223:27722-27741 | None:intergenic | 35.0% | |
| ATACCTCCCTTACAATTCAA+CGG | + | tig0030223:27838-27857 | None:intergenic | 35.0% | |
| !! | ATTGTAAGGGAGGTATTTGT+TGG | - | tig0030223:27842-27861 | Msa1442910:CDS | 35.0% |
| ! | TCTGTTGTGGAATTCTGAAA+CGG | + | tig0030223:27903-27922 | None:intergenic | 35.0% |
| GGAATAGGAAATATCTGTTG+TGG | + | tig0030223:27916-27935 | None:intergenic | 35.0% | |
| CAACCTTACAACAATCTACG+TGG | - | tig0030223:27645-27664 | Msa1442910:CDS | 40.0% | |
| ATCGCCTGTATTAAGCTCTA+CGG | - | tig0030223:27673-27692 | Msa1442910:CDS | 40.0% | |
| AAAGCCGTAGAGCTTAATAC+AGG | + | tig0030223:27680-27699 | None:intergenic | 40.0% | |
| TACAATTCAACGGTGGATGA+TGG | + | tig0030223:27828-27847 | None:intergenic | 40.0% | |
| TCATCCACCGTTGAATTGTA+AGG | - | tig0030223:27828-27847 | Msa1442910:CDS | 40.0% | |
| CATCCACCGTTGAATTGTAA+GGG | - | tig0030223:27829-27848 | Msa1442910:CDS | 40.0% | |
| CAGCCACGTAGATTGTTGTA+AGG | + | tig0030223:27651-27670 | None:intergenic | 45.0% | |
| TGTCATGATATCAACCACCG+TGG | - | tig0030223:27732-27751 | Msa1442910:CDS | 45.0% | |
| ATGATATCAACCACCGTGGT+GGG | - | tig0030223:27736-27755 | Msa1442910:CDS | 45.0% | |
| CATTAGGATAAAGCCCACCA+CGG | + | tig0030223:27752-27771 | None:intergenic | 45.0% | |
| !! | AAGGGAGGTATTTGTTGGCA+CGG | - | tig0030223:27847-27866 | Msa1442910:CDS | 45.0% |
| GACAACAAAAACGCCGGTGA+AGG | + | tig0030223:27716-27735 | None:intergenic | 50.0% | |
| CATGATATCAACCACCGTGG+TGG | - | tig0030223:27735-27754 | Msa1442910:CDS | 50.0% | |
| !! | CGTGGTGGGCTTTATCCTAA+TGG | - | tig0030223:27750-27769 | Msa1442910:CDS | 50.0% |
| !! | GTAGCGGTTGATAGCATACG+TGG | + | tig0030223:27806-27825 | None:intergenic | 50.0% |
| ! | TCAACGGTGGATGATGGTAG+CGG | + | tig0030223:27822-27841 | None:intergenic | 50.0% |
| CCTCCCTTACAATTCAACGG+TGG | + | tig0030223:27835-27854 | None:intergenic | 50.0% | |
| CCACCGTTGAATTGTAAGGG+AGG | - | tig0030223:27832-27851 | Msa1442910:CDS | 50.0% | |
| ! | TAGAAAGCGGCGTTGGGAAT+AGG | + | tig0030223:27931-27950 | None:intergenic | 50.0% |
| CTCTGTGCTCGTACCTTCAC+CGG | - | tig0030223:27700-27719 | Msa1442910:CDS | 55.0% | |
| TAGGATAAAGCCCACCACGG+TGG | + | tig0030223:27749-27768 | None:intergenic | 55.0% | |
| TCCTAATGGCAACGCTGACG+TGG | - | tig0030223:27764-27783 | Msa1442910:CDS | 55.0% | |
| CCTAATGGCAACGCTGACGT+GGG | - | tig0030223:27765-27784 | Msa1442910:CDS | 55.0% | |
| CTGAAACGGACCTGAGAAGC+CGG | + | tig0030223:27889-27908 | None:intergenic | 55.0% | |
| CCCACGTCAGCGTTGCCATT+AGG | + | tig0030223:27768-27787 | None:intergenic | 60.0% | |
| CTGAGAAGCCGGTGATCGGA+CGG | + | tig0030223:27878-27897 | None:intergenic | 60.0% | |
| GGACCTGAGAAGCCGGTGAT+CGG | + | tig0030223:27882-27901 | None:intergenic | 60.0% | |
| !! | CGTCCGATCACCGGCTTCTC+AGG | - | tig0030223:27876-27895 | Msa1442910:CDS | 65.0% |
| !! | CGGTGTCGCCGTCCGATCAC+CGG | - | tig0030223:27867-27886 | Msa1442910:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0030223 | gene | 27619 | 27951 | 27619 | ID=Msa1442910;Name=Msa1442910 |
| tig0030223 | mRNA | 27619 | 27951 | 27619 | ID=Msa1442910-mRNA-1;Parent=Msa1442910;Name=Msa1442910-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|110 |
| tig0030223 | exon | 27619 | 27951 | 27619 | ID=Msa1442910-mRNA-1:exon:13609;Parent=Msa1442910-mRNA-1 |
| tig0030223 | CDS | 27619 | 27951 | 27619 | ID=Msa1442910-mRNA-1:cds;Parent=Msa1442910-mRNA-1 |
| Gene Sequence |
| Protein sequence |
