AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0666580


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0666580 MtrunA17_Chr4g0005131 97.396 192 5 0 1 192 1 192 2.42e-137 381
Msa0666580 MtrunA17_Chr4g0005111 68.817 186 56 1 6 191 7 190 1.80e-89 260
Msa0666580 MtrunA17_Chr4g0005091 63.441 186 53 2 6 191 5 175 5.74e-77 228
Msa0666580 MtrunA17_Chr4g0005081 61.413 184 65 3 13 191 15 197 8.48e-71 213
Msa0666580 MtrunA17_Chr4g0005071 60.109 183 68 3 13 191 15 196 2.21e-68 207
Msa0666580 MtrunA17_Chr7g0243501 57.447 188 75 2 7 191 7 192 8.58e-67 202
Msa0666580 MtrunA17_Chr4g0005041 61.047 172 60 3 24 191 30 198 5.84e-66 201
Msa0666580 MtrunA17_Chr4g0005031 56.497 177 74 2 17 191 46 221 5.40e-65 199
Msa0666580 MtrunA17_Chr4g0005011 57.143 168 66 4 24 188 30 194 3.66e-62 191
Msa0666580 MtrunA17_Chr1g0175911 47.668 193 91 5 6 191 3 192 3.83e-54 171
Msa0666580 MtrunA17_Chr7g0259071 42.077 183 99 5 11 191 14 191 2.47e-47 153
Msa0666580 MtrunA17_Chr7g0259091 39.773 176 103 3 18 191 20 194 8.26e-44 144
Msa0666580 MtrunA17_Chr4g0037481 42.105 152 88 0 40 191 41 192 5.49e-43 142
Msa0666580 MtrunA17_Chr7g0259101 40.909 176 101 3 18 191 20 194 1.46e-42 146
Msa0666580 MtrunA17_Chr7g0259101 38.068 176 106 3 18 191 219 393 1.56e-40 141
Msa0666580 MtrunA17_Chr1g0177051 45.395 152 80 3 40 190 36 185 2.27e-42 140
Msa0666580 MtrunA17_Chr7g0259121 42.857 168 91 3 29 191 26 193 2.25e-40 136
Msa0666580 MtrunA17_Chr3g0085311 40.306 196 103 4 1 190 1 188 2.48e-36 125
Msa0666580 MtrunA17_Chr3g0085291 38.860 193 108 4 1 190 1 186 5.38e-35 121
Msa0666580 MtrunA17_Chr3g0136641 40.373 161 93 2 32 191 30 188 8.60e-35 121
Msa0666580 MtrunA17_Chr5g0446751 39.267 191 102 5 7 191 7 189 2.94e-33 117
Msa0666580 MtrunA17_Chr3g0136631 36.269 193 112 4 4 191 3 189 2.97e-33 117
Msa0666580 MtrunA17_Chr3g0085321 35.897 195 113 5 1 190 1 188 1.48e-32 115
Msa0666580 MtrunA17_Chr3g0085301 40.411 146 84 2 45 190 51 193 2.08e-31 112
Msa0666580 MtrunA17_Chr4g0040551 36.735 147 90 1 45 191 42 185 5.98e-30 108
Msa0666580 MtrunA17_Chr3g0090231 34.254 181 115 2 12 191 8 185 7.41e-30 108
Msa0666580 MtrunA17_Chr4g0024951 34.483 145 88 2 45 186 33 173 1.14e-22 89.7
Msa0666580 MtrunA17_Chr4g0068391 37.500 144 87 2 49 191 39 180 2.62e-22 89.0
Msa0666580 MtrunA17_Chr4g0024961 33.793 145 89 2 45 186 33 173 1.43e-21 87.0
Msa0666580 MtrunA17_Chr4g0068381 32.515 163 106 2 29 191 22 180 4.42e-21 85.5
Msa0666580 MtrunA17_Chr8g0387711 32.298 161 83 5 30 174 18 168 9.61e-14 66.2
Msa0666580 MtrunA17_Chr1g0169281 38.298 94 57 1 78 171 141 233 5.69e-13 65.5
Msa0666580 MtrunA17_Chr1g0169511 39.362 94 56 1 78 171 135 227 1.89e-12 63.9
Msa0666580 MtrunA17_Chr1g0169291 39.362 94 56 1 78 171 135 227 2.05e-12 63.9
Msa0666580 MtrunA17_Chr8g0387701 33.333 135 78 4 49 174 41 172 1.36e-11 60.8
Msa0666580 MtrunA17_Chr8g0370501 26.056 142 103 1 46 187 42 181 6.93e-11 58.9
Msa0666580 MtrunA17_Chr8g0370591 26.056 142 103 1 46 187 42 181 7.59e-11 58.5
Msa0666580 MtrunA17_Chr1g0212861 34.711 121 68 4 79 190 235 353 9.04e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0666580 AT1G58170.1 53.476 187 74 4 10 191 7 185 5.44e-65 198
Msa0666580 AT1G55210.1 51.323 189 85 5 6 191 3 187 4.05e-57 178
Msa0666580 AT1G55210.2 51.323 189 85 5 6 191 3 187 4.05e-57 178
Msa0666580 AT5G49040.1 50.847 177 84 2 17 191 16 191 9.09e-56 175
Msa0666580 AT3G13650.1 51.445 173 79 4 20 191 18 186 8.04e-53 167
Msa0666580 AT3G13662.1 49.425 174 78 5 7 178 7 172 5.29e-47 152
Msa0666580 AT1G65870.1 47.260 146 76 1 45 190 45 189 2.95e-45 148
Msa0666580 AT5G49030.3 50.602 166 79 2 17 180 16 180 3.49e-44 157
Msa0666580 AT3G13660.1 57.143 119 51 0 73 191 7 125 2.63e-42 139
Msa0666580 AT5G42500.1 41.818 165 93 2 26 190 24 185 1.80e-40 136
Msa0666580 AT1G22900.1 46.575 146 75 2 45 190 51 193 4.39e-39 132
Msa0666580 AT5G42510.1 41.875 160 90 2 31 190 26 182 5.06e-39 132
Msa0666580 AT2G21100.1 41.447 152 88 1 39 190 37 187 6.59e-38 129
Msa0666580 AT2G21100.2 41.447 152 88 1 39 190 37 187 6.59e-38 129
Msa0666580 AT3G58090.1 46.364 110 59 0 81 190 162 271 2.17e-32 117
Msa0666580 AT4G38700.1 34.694 196 117 5 1 191 1 190 1.99e-28 105
Msa0666580 AT2G21110.1 32.432 185 116 4 11 191 7 186 5.73e-28 103
Msa0666580 AT4G23690.1 36.029 136 77 5 36 166 32 162 2.24e-15 71.2
Msa0666580 AT4G11210.1 31.579 171 103 6 12 178 7 167 4.18e-14 67.8
Msa0666580 AT4G11190.1 30.935 139 85 4 44 178 36 167 1.42e-12 63.5

Find 48 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTAAAAGAGTTGTTAAATT+TGG 0.090030 4_4:+6670921 None:intergenic
AAGTTTGGTGTCAAGGTTAA+AGG 0.229203 4_4:+6670868 None:intergenic
AAGTAGCTGCTAGCCACTTT+AGG 0.300872 4_4:-6671008 Msa0666580:CDS
CTAAAAGAGTTGTTAAATTT+GGG 0.308915 4_4:+6670922 None:intergenic
TTGGGAAGTGGTTGAATGAT+TGG 0.327500 4_4:+6670940 None:intergenic
TTGTTTGAAGGAAAGTATAA+TGG 0.337558 4_4:-6670757 Msa0666580:CDS
GGGTGTAGAAAGAGAGTAGA+AGG 0.394648 4_4:+6671100 None:intergenic
GGGAGTACCATTACTATCTC+TGG 0.407901 4_4:-6670736 Msa0666580:CDS
ATGAACTTTGCCTTGTTTGA+AGG 0.417944 4_4:-6670769 Msa0666580:CDS
TGTTTGAAGGAAAGTATAAT+GGG 0.433452 4_4:-6670756 Msa0666580:CDS
CAGCATATCCCTTAGCAAAT+CGG 0.436333 4_4:+6670653 None:intergenic
ATTTATTGAGCTTACAAAAC+CGG 0.438981 4_4:+6671050 None:intergenic
AGTTTGGTGTCAAGGTTAAA+GGG 0.445162 4_4:+6670869 None:intergenic
TACCAGTGATCGGTGGTAGC+GGG 0.446390 4_4:-6670681 Msa0666580:CDS
CTAGCCACTTTAGGTTCTAC+TGG 0.448964 4_4:-6670999 Msa0666580:CDS
AACTTGACCTCAAAATTGTT+AGG 0.452723 4_4:-6670850 Msa0666580:CDS
TACTTTCTTTGTAAGCAACT+TGG 0.452780 4_4:+6671026 None:intergenic
AAGGAACGTCGCAAACGATA+AGG 0.481824 4_4:-6670713 Msa0666580:CDS
ACTTTCTTTGTAAGCAACTT+GGG 0.483229 4_4:+6671027 None:intergenic
TGACCTCAAAATTGTTAGGT+AGG 0.497868 4_4:-6670846 Msa0666580:CDS
TACACCCTCACAATTGTCAC+TGG 0.498118 4_4:-6671084 Msa0666580:CDS
AAATTGTTAGGTAGGGCTCA+AGG 0.500966 4_4:-6670838 Msa0666580:CDS
TGAGGTCAAGTTTGGTGTCA+AGG 0.506209 4_4:+6670861 None:intergenic
GAGTTGTTAAATTTGGGAAG+TGG 0.508093 4_4:+6670928 None:intergenic
GTTCTACTGGCAAGACATAG+TGG 0.508556 4_4:-6670986 Msa0666580:CDS
ACCCCGCTACCACCGATCAC+TGG 0.532338 4_4:+6670679 None:intergenic
TTACCAGTGATCGGTGGTAG+CGG 0.542224 4_4:-6670682 Msa0666580:CDS
AATTGTTAGGTAGGGCTCAA+GGG 0.543701 4_4:-6670837 Msa0666580:CDS
TGGTTGAATGATTGGAATGG+AGG 0.546721 4_4:+6670948 None:intergenic
GTATTCAACCACGGTATCCC+CGG 0.549581 4_4:+6670600 None:intergenic
CTTGCCAGTAGAACCTAAAG+TGG 0.555578 4_4:+6670995 None:intergenic
GACCTCAAAATTGTTAGGTA+GGG 0.562175 4_4:-6670845 Msa0666580:CDS
ACCAGTGATCGGTGGTAGCG+GGG 0.577667 4_4:-6670680 Msa0666580:CDS
GTCACTGGACAAGATGAGAC+CGG 0.584277 4_4:-6671069 Msa0666580:CDS
AAGTGGTTGAATGATTGGAA+TGG 0.586361 4_4:+6670945 None:intergenic
ATTATACTTTCCTTCAAACA+AGG 0.587601 4_4:+6670759 None:intergenic
AACCATAAGAAAATCAAGCT+CGG 0.592742 4_4:+6670792 None:intergenic
GTTAGAGAGTTACCAGTGAT+CGG 0.602234 4_4:-6670691 Msa0666580:CDS
TTCTACTGGCAAGACATAGT+GGG 0.624056 4_4:-6670985 Msa0666580:CDS
TTTCATAACCGGGGATACCG+TGG 0.641517 4_4:-6670608 Msa0666580:CDS
GTACCATTACTATCTCTGGA+AGG 0.647934 4_4:-6670732 Msa0666580:CDS
ATAAACATTGTATTCAACCA+CGG 0.650255 4_4:+6670591 None:intergenic
GTTCCTTCCAGAGATAGTAA+TGG 0.662479 4_4:+6670729 None:intergenic
AAGCTCGGTTTGCGATGTAG+CGG 0.665030 4_4:+6670807 None:intergenic
TACTGGCAAGACATAGTGGG+AGG 0.669570 4_4:-6670982 Msa0666580:CDS
CTTGTCCAGTGACAATTGTG+AGG 0.674513 4_4:+6671079 None:intergenic
TTGTCCAGTGACAATTGTGA+GGG 0.677874 4_4:+6671080 None:intergenic
AGAGAGTTACCAGTGATCGG+TGG 0.703961 4_4:-6670688 Msa0666580:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGGAAGAAAAAAAGTTTTT+GGG + chr4_4:6670586-6670605 None:intergenic 20.0%
!! CTAAAAGAGTTGTTAAATTT+GGG + chr4_4:6670783-6670802 None:intergenic 20.0%
!! ACTAAAAGAGTTGTTAAATT+TGG + chr4_4:6670784-6670803 None:intergenic 20.0%
!! GAAGGAAGAAAAAAAGTTTT+TGG + chr4_4:6670587-6670606 None:intergenic 25.0%
! ATTTATTGAGCTTACAAAAC+CGG + chr4_4:6670655-6670674 None:intergenic 25.0%
! ATTATACTTTCCTTCAAACA+AGG + chr4_4:6670946-6670965 None:intergenic 25.0%
!! TTGTTTGAAGGAAAGTATAA+TGG - chr4_4:6670945-6670964 Msa0666580:CDS 25.0%
!! TGTTTGAAGGAAAGTATAAT+GGG - chr4_4:6670946-6670965 Msa0666580:CDS 25.0%
!!! ATCTCTTTTGATTTCATAAC+CGG - chr4_4:6671083-6671102 Msa0666580:CDS 25.0%
! ATAAACATTGTATTCAACCA+CGG + chr4_4:6671114-6671133 None:intergenic 25.0%
ACTTTCTTTGTAAGCAACTT+GGG + chr4_4:6670678-6670697 None:intergenic 30.0%
TACTTTCTTTGTAAGCAACT+TGG + chr4_4:6670679-6670698 None:intergenic 30.0%
! AACAACTCTTTTAGTGCTTT+TGG - chr4_4:6670789-6670808 Msa0666580:CDS 30.0%
!!! AACAATTTTGAGGTCAAGTT+TGG + chr4_4:6670852-6670871 None:intergenic 30.0%
AACTTGACCTCAAAATTGTT+AGG - chr4_4:6670852-6670871 Msa0666580:CDS 30.0%
AACCATAAGAAAATCAAGCT+CGG + chr4_4:6670913-6670932 None:intergenic 30.0%
!!! TCTCTTTTGATTTCATAACC+GGG - chr4_4:6671084-6671103 Msa0666580:CDS 30.0%
AAGTGGTTGAATGATTGGAA+TGG + chr4_4:6670760-6670779 None:intergenic 35.0%
GAGTTGTTAAATTTGGGAAG+TGG + chr4_4:6670777-6670796 None:intergenic 35.0%
!! CTCTTTTAGTGCTTTTGGTT+TGG - chr4_4:6670794-6670813 Msa0666580:CDS 35.0%
!! AGTTTGGTGTCAAGGTTAAA+GGG + chr4_4:6670836-6670855 None:intergenic 35.0%
!! AAGTTTGGTGTCAAGGTTAA+AGG + chr4_4:6670837-6670856 None:intergenic 35.0%
TGACCTCAAAATTGTTAGGT+AGG - chr4_4:6670856-6670875 Msa0666580:CDS 35.0%
GACCTCAAAATTGTTAGGTA+GGG - chr4_4:6670857-6670876 Msa0666580:CDS 35.0%
! AACCGAGCTTGATTTTCTTA+TGG - chr4_4:6670908-6670927 Msa0666580:CDS 35.0%
!! ATGAACTTTGCCTTGTTTGA+AGG - chr4_4:6670933-6670952 Msa0666580:CDS 35.0%
!!! CTCTTTTGATTTCATAACCG+GGG - chr4_4:6671085-6671104 Msa0666580:CDS 35.0%
TTGTCCAGTGACAATTGTGA+GGG + chr4_4:6670625-6670644 None:intergenic 40.0%
TTCTACTGGCAAGACATAGT+GGG - chr4_4:6670717-6670736 Msa0666580:CDS 40.0%
TGGTTGAATGATTGGAATGG+AGG + chr4_4:6670757-6670776 None:intergenic 40.0%
TTGGGAAGTGGTTGAATGAT+TGG + chr4_4:6670765-6670784 None:intergenic 40.0%
!!! TTAGTGCTTTTGGTTTGGTG+AGG - chr4_4:6670799-6670818 Msa0666580:CDS 40.0%
! AGCCCTACCTAACAATTTTG+AGG + chr4_4:6670862-6670881 None:intergenic 40.0%
AAATTGTTAGGTAGGGCTCA+AGG - chr4_4:6670864-6670883 Msa0666580:CDS 40.0%
AATTGTTAGGTAGGGCTCAA+GGG - chr4_4:6670865-6670884 Msa0666580:CDS 40.0%
GTACCATTACTATCTCTGGA+AGG - chr4_4:6670970-6670989 Msa0666580:CDS 40.0%
GTTCCTTCCAGAGATAGTAA+TGG + chr4_4:6670976-6670995 None:intergenic 40.0%
GTTAGAGAGTTACCAGTGAT+CGG - chr4_4:6671011-6671030 Msa0666580:CDS 40.0%
CAGCATATCCCTTAGCAAAT+CGG + chr4_4:6671052-6671071 None:intergenic 40.0%
GGGTGTAGAAAGAGAGTAGA+AGG + chr4_4:6670605-6670624 None:intergenic 45.0%
TACACCCTCACAATTGTCAC+TGG - chr4_4:6670618-6670637 Msa0666580:CDS 45.0%
CTTGTCCAGTGACAATTGTG+AGG + chr4_4:6670626-6670645 None:intergenic 45.0%
! AAGTAGCTGCTAGCCACTTT+AGG - chr4_4:6670694-6670713 Msa0666580:CDS 45.0%
! CTAGCCACTTTAGGTTCTAC+TGG - chr4_4:6670703-6670722 Msa0666580:CDS 45.0%
CTTGCCAGTAGAACCTAAAG+TGG + chr4_4:6670710-6670729 None:intergenic 45.0%
GTTCTACTGGCAAGACATAG+TGG - chr4_4:6670716-6670735 Msa0666580:CDS 45.0%
!! TGAGGTCAAGTTTGGTGTCA+AGG + chr4_4:6670844-6670863 None:intergenic 45.0%
!! GGGAGTACCATTACTATCTC+TGG - chr4_4:6670966-6670985 Msa0666580:CDS 45.0%
AAGGAACGTCGCAAACGATA+AGG - chr4_4:6670989-6671008 Msa0666580:CDS 45.0%
!!! GGGGTTTTCCGATTTGCTAA+GGG - chr4_4:6671041-6671060 Msa0666580:CDS 45.0%
GTCACTGGACAAGATGAGAC+CGG - chr4_4:6670633-6670652 Msa0666580:CDS 50.0%
! TACTGGCAAGACATAGTGGG+AGG - chr4_4:6670720-6670739 Msa0666580:CDS 50.0%
! AAGCTCGGTTTGCGATGTAG+CGG + chr4_4:6670898-6670917 None:intergenic 50.0%
AGAGAGTTACCAGTGATCGG+TGG - chr4_4:6671014-6671033 Msa0666580:CDS 50.0%
TTACCAGTGATCGGTGGTAG+CGG - chr4_4:6671020-6671039 Msa0666580:CDS 50.0%
!!! CGGGGTTTTCCGATTTGCTA+AGG - chr4_4:6671040-6671059 Msa0666580:CDS 50.0%
TTTCATAACCGGGGATACCG+TGG - chr4_4:6671094-6671113 Msa0666580:CDS 50.0%
GTATTCAACCACGGTATCCC+CGG + chr4_4:6671105-6671124 None:intergenic 50.0%
TACCAGTGATCGGTGGTAGC+GGG - chr4_4:6671021-6671040 Msa0666580:CDS 55.0%
! ACCAGTGATCGGTGGTAGCG+GGG - chr4_4:6671022-6671041 Msa0666580:CDS 60.0%
ACCCCGCTACCACCGATCAC+TGG + chr4_4:6671026-6671045 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 6670573 6671151 6670573 ID=Msa0666580;Name=Msa0666580
chr4_4 mRNA 6670573 6671151 6670573 ID=Msa0666580-mRNA-1;Parent=Msa0666580;Name=Msa0666580-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|192
chr4_4 exon 6670573 6671151 6670573 ID=Msa0666580-mRNA-1:exon:2154;Parent=Msa0666580-mRNA-1
chr4_4 CDS 6670573 6671151 6670573 ID=Msa0666580-mRNA-1:cds;Parent=Msa0666580-mRNA-1
Gene Sequence

>Msa0666580

ATGAATACTTCCCAAAAACTTTTTTTCTTCCTTCTACTCTCTTTCTACACCCTCACAATTGTCACTGGACAAGATGAGACCGGTTTTGTAAGCTCAATAAATCCCAAGTTGCTTACAAAGAAAGTAGCTGCTAGCCACTTTAGGTTCTACTGGCAAGACATAGTGGGAGGAGCAAACGCAACCTCCATTCCAATCATTCAACCACTTCCCAAATTTAACAACTCTTTTAGTGCTTTTGGTTTGGTGAGGATAATCGACAACCCTTTAACCTTGACACCAAACTTGACCTCAAAATTGTTAGGTAGGGCTCAAGGGTTTTATGCCGCTACATCGCAAACCGAGCTTGATTTTCTTATGGTTATGAACTTTGCCTTGTTTGAAGGAAAGTATAATGGGAGTACCATTACTATCTCTGGAAGGAACGTCGCAAACGATAAGGTTAGAGAGTTACCAGTGATCGGTGGTAGCGGGGTTTTCCGATTTGCTAAGGGATATGCTGAAGCTAGAACAATCTCTTTTGATTTCATAACCGGGGATACCGTGGTTGAATACAATGTTTATGTTTCTCATCATAAATGA

Protein sequence

>Msa0666580

MNTSQKLFFFLLLSFYTLTIVTGQDETGFVSSINPKLLTKKVAASHFRFYWQDIVGGANATSIPIIQPLPKFNNSFSAFGLVRIIDNPLTLTPNLTSKLLGRAQGFYAATSQTELDFLMVMNFALFEGKYNGSTITISGRNVANDKVRELPVIGGSGVFRFAKGYAEARTISFDFITGDTVVEYNVYVSHHK*