AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0004280

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0004280 MtrunA17_Chr1g0156831 86.792 106 13 1 1 106 70 174 3.47e-49 154
Msa0004280 MtrunA17_Chr7g0214971 83.962 106 16 1 1 106 75 179 8.01e-46 145
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0004280 AT5G49210.2 53.846 104 47 1 3 106 93 195 3.20e-27 99.4
Msa0004280 AT5G49210.1 53.846 104 47 1 3 106 93 195 3.20e-27 99.4

Find 44 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGCAGAGAAACTTGAAATT+TGG 0.193752 1_1:-6699192 Msa0004280:CDS
TGCAGCAAGGTTTGTAATTC+AGG 0.202862 1_1:+6698857 None:intergenic
CGTGATTTCCTTCTCGAGTT+CGG 0.341961 1_1:+6699081 None:intergenic
TCGGCTTCCTTAGGATCCTT+TGG 0.349655 1_1:+6699993 None:intergenic
ATTTGAATTAACAAAACGTT+AGG 0.356278 1_1:+6698734 None:intergenic
CATGAACATAAGTCTTTAGT+TGG 0.357818 1_1:-6698917 Msa0004280:three_prime_UTR
AAAGAGGTTCGGAAGGAATA+TGG 0.366632 1_1:-6699948 Msa0004280:exon
CTCTCACCAAGCTCCTTTGT+TGG 0.389335 1_1:-6700029 Msa0004280:five_prime_UTR
CCTGAGCCTTAAGTTTCTTA+CGG 0.429159 1_1:+6699864 None:intergenic
TGAAGACCGTAAGAAACTTA+AGG 0.443901 1_1:-6699870 Msa0004280:CDS
TTTGAATTAACAAAACGTTA+GGG 0.449534 1_1:+6698735 None:intergenic
CAAGCTCCTTTGTTGGTAGC+TGG 0.464757 1_1:-6700022 Msa0004280:five_prime_UTR
AGCATTGCTTCGGCTTCCTT+AGG 0.466457 1_1:+6699984 None:intergenic
TTGCTGAGCAAGCATTGCTT+CGG 0.483470 1_1:+6699974 None:intergenic
TTCACGGTGGTTGTTGTGGT+GGG 0.483941 1_1:+6700064 None:intergenic
GTTAATTCAAATGAGTTGCT+TGG 0.485144 1_1:-6698723 Msa0004280:three_prime_UTR
CTCAGCAAGTGAAAGAGGTT+CGG 0.486656 1_1:-6699959 Msa0004280:five_prime_UTR
GGCTGCAGAACTTAGAGCTC+AGG 0.488486 1_1:-6699843 Msa0004280:CDS
AGCAACGCAAGGACGAGGCT+CGG 0.500025 1_1:-6699916 Msa0004280:CDS
TGATGTAGATTCCCGTCTGA+TGG 0.504577 1_1:-6698794 Msa0004280:three_prime_UTR
CCGTAAGAAACTTAAGGCTC+AGG 0.511709 1_1:-6699864 Msa0004280:CDS
AGTTGTAGCAACTACAACAT+TGG 0.520406 1_1:+6698985 None:intergenic
ACGCAAGGACGAGGCTCGGA+GGG 0.529474 1_1:-6699912 Msa0004280:CDS
GCTTGCTCAGCAAGTGAAAG+AGG 0.530883 1_1:-6699964 Msa0004280:five_prime_UTR
GCAAGCTCCAAAGGATCCTA+AGG 0.534673 1_1:-6700000 Msa0004280:five_prime_UTR
TGGTAGCTGGCAAGCTCCAA+AGG 0.552738 1_1:-6700009 Msa0004280:five_prime_UTR
TTTCACGGTGGTTGTTGTGG+TGG 0.555232 1_1:+6700063 None:intergenic
GTAGAGATATGAAACTGCAT+GGG 0.557174 1_1:+6699026 None:intergenic
AGTAGAGATATGAAACTGCA+TGG 0.566453 1_1:+6699025 None:intergenic
GTTCGGAAGGAATATGGCGT+TGG 0.570535 1_1:-6699942 Msa0004280:exon
TTGAATTAACAAAACGTTAG+GGG 0.577715 1_1:+6698736 None:intergenic
CAGCTACCAACAAAGGAGCT+TGG 0.578257 1_1:+6700023 None:intergenic
GCATAAACAAAGTTCACTGT+GGG 0.580460 1_1:-6699113 Msa0004280:CDS
GGAGAAGCAACGCAAGGACG+AGG 0.585661 1_1:-6699921 Msa0004280:CDS
AAAGCATGAAGAGAAGAAGA+AGG 0.593300 1_1:-6699155 Msa0004280:CDS
TGATGAAGCCGAACTCGAGA+AGG 0.603871 1_1:-6699089 Msa0004280:CDS
AACGCAAGGACGAGGCTCGG+AGG 0.611133 1_1:-6699913 Msa0004280:CDS
AGCTTGCCAGCTACCAACAA+AGG 0.613997 1_1:+6700016 None:intergenic
GCAAGTGAAAGAGGTTCGGA+AGG 0.620900 1_1:-6699955 Msa0004280:five_prime_UTR
GGCGTTGGAGAAGCAACGCA+AGG 0.639434 1_1:-6699927 Msa0004280:CDS
TGCATAAACAAAGTTCACTG+TGG 0.647677 1_1:-6699114 Msa0004280:CDS
CAACAAAGGAGCTTGGTGAG+AGG 0.649374 1_1:+6700030 None:intergenic
GACTTGGTAGAACAATCAAG+TGG 0.694641 1_1:-6698823 Msa0004280:three_prime_UTR
TAGAGATATGAAACTGCATG+GGG 0.714260 1_1:+6699027 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATAAGTATTAAATCAAAA+AGG + chr1_1:6699372-6699391 None:intergenic 10.0%
!!! TTTTTGATTTAATACTTATA+TGG - chr1_1:6699371-6699390 Msa0004280:intron 10.0%
!!! TATTTGTTAAAAAATTTCTA+AGG - chr1_1:6699559-6699578 Msa0004280:intron 10.0%
!! ATATAAGTATTAAATCAAAA+AGG + chr1_1:6699372-6699391 None:intergenic 10.0%
!!! TTTTTGATTTAATACTTATA+TGG - chr1_1:6699371-6699390 Msa0004280:intron 10.0%
!!! TATTTGTTAAAAAATTTCTA+AGG - chr1_1:6699559-6699578 Msa0004280:intron 10.0%
!!! AAATGAATTGATTTACAATT+TGG - chr1_1:6699199-6699218 Msa0004280:CDS 15.0%
!!! AAATGAATTGATTTACAATT+TGG - chr1_1:6699199-6699218 Msa0004280:CDS 15.0%
!! AGTATAGCAAGAAAAAATTT+GGG + chr1_1:6698746-6698765 None:intergenic 20.0%
!!! TCCTTTTCAATTTCTTATTA+TGG - chr1_1:6699120-6699139 Msa0004280:CDS 20.0%
!! TCCATAATAAGAAATTGAAA+AGG + chr1_1:6699124-6699143 None:intergenic 20.0%
!! TATTTAAGCATTTAAACAAC+TGG - chr1_1:6699248-6699267 Msa0004280:intron 20.0%
!!! TTTTTTTCTTCGAATTTTGA+AGG - chr1_1:6700008-6700027 Msa0004280:five_prime_UTR 20.0%
!! TTTGAATTAACAAAACGTTA+GGG + chr1_1:6700163-6700182 None:intergenic 20.0%
!! ATTTGAATTAACAAAACGTT+AGG + chr1_1:6700164-6700183 None:intergenic 20.0%
!! AGTATAGCAAGAAAAAATTT+GGG + chr1_1:6698746-6698765 None:intergenic 20.0%
!!! TCCTTTTCAATTTCTTATTA+TGG - chr1_1:6699120-6699139 Msa0004280:CDS 20.0%
!! TCCATAATAAGAAATTGAAA+AGG + chr1_1:6699124-6699143 None:intergenic 20.0%
!! TATTTAAGCATTTAAACAAC+TGG - chr1_1:6699248-6699267 Msa0004280:intron 20.0%
!!! TTTTTTTCTTCGAATTTTGA+AGG - chr1_1:6700008-6700027 Msa0004280:five_prime_UTR 20.0%
!! TTTGAATTAACAAAACGTTA+GGG + chr1_1:6700163-6700182 None:intergenic 20.0%
!! ATTTGAATTAACAAAACGTT+AGG + chr1_1:6700164-6700183 None:intergenic 20.0%
! CAGTATAGCAAGAAAAAATT+TGG + chr1_1:6698747-6698766 None:intergenic 25.0%
! ATAAGTTGTTTAACCAAACA+GGG + chr1_1:6699231-6699250 None:intergenic 25.0%
! AATAAGTTGTTTAACCAAAC+AGG + chr1_1:6699232-6699251 None:intergenic 25.0%
!!! ACTTCTCACATTTTTTTTAG+AGG + chr1_1:6699324-6699343 None:intergenic 25.0%
! ATGAAATCTAGTTAACTGTA+AGG + chr1_1:6699523-6699542 None:intergenic 25.0%
! TCATTTAAAAAATTGCAGCA+AGG + chr1_1:6700054-6700073 None:intergenic 25.0%
!!! CTGCAATTTTTTAAATGACT+TGG - chr1_1:6700056-6700075 Msa0004280:five_prime_UTR 25.0%
! TTGAATTAACAAAACGTTAG+GGG + chr1_1:6700162-6700181 None:intergenic 25.0%
! CAGTATAGCAAGAAAAAATT+TGG + chr1_1:6698747-6698766 None:intergenic 25.0%
! ATAAGTTGTTTAACCAAACA+GGG + chr1_1:6699231-6699250 None:intergenic 25.0%
! AATAAGTTGTTTAACCAAAC+AGG + chr1_1:6699232-6699251 None:intergenic 25.0%
!!! ACTTCTCACATTTTTTTTAG+AGG + chr1_1:6699324-6699343 None:intergenic 25.0%
! ATGAAATCTAGTTAACTGTA+AGG + chr1_1:6699523-6699542 None:intergenic 25.0%
! TCATTTAAAAAATTGCAGCA+AGG + chr1_1:6700054-6700073 None:intergenic 25.0%
!!! CTGCAATTTTTTAAATGACT+TGG - chr1_1:6700056-6700075 Msa0004280:five_prime_UTR 25.0%
! TTGAATTAACAAAACGTTAG+GGG + chr1_1:6700162-6700181 None:intergenic 25.0%
ATTGAAAAGGAGACTAACAA+GGG + chr1_1:6699111-6699130 None:intergenic 30.0%
AATTGAAAAGGAGACTAACA+AGG + chr1_1:6699112-6699131 None:intergenic 30.0%
GACATGATAAATGTATTCAG+GGG + chr1_1:6699302-6699321 None:intergenic 30.0%
GGACATGATAAATGTATTCA+GGG + chr1_1:6699303-6699322 None:intergenic 30.0%
AGGACATGATAAATGTATTC+AGG + chr1_1:6699304-6699323 None:intergenic 30.0%
!! ACCTACTTTTATCGTAGTTA+AGG - chr1_1:6699394-6699413 Msa0004280:intron 30.0%
ACCTTAACTACGATAAAAGT+AGG + chr1_1:6699398-6699417 None:intergenic 30.0%
! TACTTTTATCGTAGTTAAGG+TGG - chr1_1:6699397-6699416 Msa0004280:intron 30.0%
! ATTTCACTTTGATAATCTGC+AGG - chr1_1:6699420-6699439 Msa0004280:intron 30.0%
CATGAACATAAGTCTTTAGT+TGG - chr1_1:6699978-6699997 Msa0004280:five_prime_UTR 30.0%
GTTAATTCAAATGAGTTGCT+TGG - chr1_1:6700172-6700191 Msa0004280:five_prime_UTR 30.0%
ATTGAAAAGGAGACTAACAA+GGG + chr1_1:6699111-6699130 None:intergenic 30.0%
AATTGAAAAGGAGACTAACA+AGG + chr1_1:6699112-6699131 None:intergenic 30.0%
GACATGATAAATGTATTCAG+GGG + chr1_1:6699302-6699321 None:intergenic 30.0%
GGACATGATAAATGTATTCA+GGG + chr1_1:6699303-6699322 None:intergenic 30.0%
AGGACATGATAAATGTATTC+AGG + chr1_1:6699304-6699323 None:intergenic 30.0%
!! ACCTACTTTTATCGTAGTTA+AGG - chr1_1:6699394-6699413 Msa0004280:intron 30.0%
ACCTTAACTACGATAAAAGT+AGG + chr1_1:6699398-6699417 None:intergenic 30.0%
! TACTTTTATCGTAGTTAAGG+TGG - chr1_1:6699397-6699416 Msa0004280:intron 30.0%
! ATTTCACTTTGATAATCTGC+AGG - chr1_1:6699420-6699439 Msa0004280:intron 30.0%
CATGAACATAAGTCTTTAGT+TGG - chr1_1:6699978-6699997 Msa0004280:five_prime_UTR 30.0%
GTTAATTCAAATGAGTTGCT+TGG - chr1_1:6700172-6700191 Msa0004280:five_prime_UTR 30.0%
!! TTTTCTTGCTATACTGTACG+TGG - chr1_1:6698751-6698770 Msa0004280:three_prime_UTR 35.0%
! AGAGGAACTTGTGATTTTCA+CGG + chr1_1:6698850-6698869 None:intergenic 35.0%
TGAAGACCGTAAGAAACTTA+AGG - chr1_1:6699025-6699044 Msa0004280:three_prime_UTR 35.0%
AATTTGGATTAAGCCCTGTT+TGG - chr1_1:6699215-6699234 Msa0004280:intron 35.0%
!! CTCTCTCTTTTAACTGATTC+AGG + chr1_1:6699686-6699705 None:intergenic 35.0%
GAGCAGAGAAACTTGAAATT+TGG - chr1_1:6699703-6699722 Msa0004280:intron 35.0%
! CTTCTTCTCTTCATGCTTTT+TGG + chr1_1:6699739-6699758 None:intergenic 35.0%
AAAGCATGAAGAGAAGAAGA+AGG - chr1_1:6699740-6699759 Msa0004280:intron 35.0%
TGCATAAACAAAGTTCACTG+TGG - chr1_1:6699781-6699800 Msa0004280:intron 35.0%
GCATAAACAAAGTTCACTGT+GGG - chr1_1:6699782-6699801 Msa0004280:intron 35.0%
TAGAGATATGAAACTGCATG+GGG + chr1_1:6699871-6699890 None:intergenic 35.0%
GTAGAGATATGAAACTGCAT+GGG + chr1_1:6699872-6699891 None:intergenic 35.0%
AGTAGAGATATGAAACTGCA+TGG + chr1_1:6699873-6699892 None:intergenic 35.0%
AGTTGTAGCAACTACAACAT+TGG + chr1_1:6699913-6699932 None:intergenic 35.0%
! TTGCAATGTTTTCCATCAGA+CGG + chr1_1:6700116-6700135 None:intergenic 35.0%
!! TTTTCTTGCTATACTGTACG+TGG - chr1_1:6698751-6698770 Msa0004280:three_prime_UTR 35.0%
! AGAGGAACTTGTGATTTTCA+CGG + chr1_1:6698850-6698869 None:intergenic 35.0%
TGAAGACCGTAAGAAACTTA+AGG - chr1_1:6699025-6699044 Msa0004280:three_prime_UTR 35.0%
AATTTGGATTAAGCCCTGTT+TGG - chr1_1:6699215-6699234 Msa0004280:intron 35.0%
!! CTCTCTCTTTTAACTGATTC+AGG + chr1_1:6699686-6699705 None:intergenic 35.0%
GAGCAGAGAAACTTGAAATT+TGG - chr1_1:6699703-6699722 Msa0004280:intron 35.0%
! CTTCTTCTCTTCATGCTTTT+TGG + chr1_1:6699739-6699758 None:intergenic 35.0%
AAAGCATGAAGAGAAGAAGA+AGG - chr1_1:6699740-6699759 Msa0004280:intron 35.0%
TGCATAAACAAAGTTCACTG+TGG - chr1_1:6699781-6699800 Msa0004280:intron 35.0%
GCATAAACAAAGTTCACTGT+GGG - chr1_1:6699782-6699801 Msa0004280:intron 35.0%
TAGAGATATGAAACTGCATG+GGG + chr1_1:6699871-6699890 None:intergenic 35.0%
GTAGAGATATGAAACTGCAT+GGG + chr1_1:6699872-6699891 None:intergenic 35.0%
AGTAGAGATATGAAACTGCA+TGG + chr1_1:6699873-6699892 None:intergenic 35.0%
AGTTGTAGCAACTACAACAT+TGG + chr1_1:6699913-6699932 None:intergenic 35.0%
! TTGCAATGTTTTCCATCAGA+CGG + chr1_1:6700116-6700135 None:intergenic 35.0%
AAAGAGGTTCGGAAGGAATA+TGG - chr1_1:6698947-6698966 Msa0004280:three_prime_UTR 40.0%
CCTGAGCCTTAAGTTTCTTA+CGG + chr1_1:6699034-6699053 None:intergenic 40.0%
!! TGTATTCAGGGGTTAGTTAC+AGG + chr1_1:6699291-6699310 None:intergenic 40.0%
TAAATACAGCACAGTCACAC+AGG + chr1_1:6699498-6699517 None:intergenic 40.0%
TGCAGCAAGGTTTGTAATTC+AGG + chr1_1:6700041-6700060 None:intergenic 40.0%
! GACTTGGTAGAACAATCAAG+TGG - chr1_1:6700072-6700091 Msa0004280:five_prime_UTR 40.0%
! TGCAATGTTTTCCATCAGAC+GGG + chr1_1:6700115-6700134 None:intergenic 40.0%
AAAGAGGTTCGGAAGGAATA+TGG - chr1_1:6698947-6698966 Msa0004280:three_prime_UTR 40.0%
CCTGAGCCTTAAGTTTCTTA+CGG + chr1_1:6699034-6699053 None:intergenic 40.0%
!! TGTATTCAGGGGTTAGTTAC+AGG + chr1_1:6699291-6699310 None:intergenic 40.0%
TAAATACAGCACAGTCACAC+AGG + chr1_1:6699498-6699517 None:intergenic 40.0%
TGCAGCAAGGTTTGTAATTC+AGG + chr1_1:6700041-6700060 None:intergenic 40.0%
! GACTTGGTAGAACAATCAAG+TGG - chr1_1:6700072-6700091 Msa0004280:five_prime_UTR 40.0%
! TGCAATGTTTTCCATCAGAC+GGG + chr1_1:6700115-6700134 None:intergenic 40.0%
! GATTTTCACGGTGGTTGTTG+TGG + chr1_1:6698838-6698857 None:intergenic 45.0%
! GGAACTTGTGATTTTCACGG+TGG + chr1_1:6698847-6698866 None:intergenic 45.0%
! TTGCTGAGCAAGCATTGCTT+CGG + chr1_1:6698924-6698943 None:intergenic 45.0%
CTCAGCAAGTGAAAGAGGTT+CGG - chr1_1:6698936-6698955 Msa0004280:three_prime_UTR 45.0%
CCGTAAGAAACTTAAGGCTC+AGG - chr1_1:6699031-6699050 Msa0004280:three_prime_UTR 45.0%
GCATGCTGTGAATCAATCTC+AGG - chr1_1:6699166-6699185 Msa0004280:CDS 45.0%
CGTGATTTCCTTCTCGAGTT+CGG + chr1_1:6699817-6699836 None:intergenic 45.0%
TGATGTAGATTCCCGTCTGA+TGG - chr1_1:6700101-6700120 Msa0004280:five_prime_UTR 45.0%
! GATTTTCACGGTGGTTGTTG+TGG + chr1_1:6698838-6698857 None:intergenic 45.0%
! GGAACTTGTGATTTTCACGG+TGG + chr1_1:6698847-6698866 None:intergenic 45.0%
! TTGCTGAGCAAGCATTGCTT+CGG + chr1_1:6698924-6698943 None:intergenic 45.0%
CTCAGCAAGTGAAAGAGGTT+CGG - chr1_1:6698936-6698955 Msa0004280:three_prime_UTR 45.0%
CCGTAAGAAACTTAAGGCTC+AGG - chr1_1:6699031-6699050 Msa0004280:three_prime_UTR 45.0%
GCATGCTGTGAATCAATCTC+AGG - chr1_1:6699166-6699185 Msa0004280:CDS 45.0%
CGTGATTTCCTTCTCGAGTT+CGG + chr1_1:6699817-6699836 None:intergenic 45.0%
TGATGTAGATTCCCGTCTGA+TGG - chr1_1:6700101-6700120 Msa0004280:five_prime_UTR 45.0%
TTCACGGTGGTTGTTGTGGT+GGG + chr1_1:6698834-6698853 None:intergenic 50.0%
TTTCACGGTGGTTGTTGTGG+TGG + chr1_1:6698835-6698854 None:intergenic 50.0%
! CAACAAAGGAGCTTGGTGAG+AGG + chr1_1:6698868-6698887 None:intergenic 50.0%
CTCTCACCAAGCTCCTTTGT+TGG - chr1_1:6698866-6698885 Msa0004280:three_prime_UTR 50.0%
CAGCTACCAACAAAGGAGCT+TGG + chr1_1:6698875-6698894 None:intergenic 50.0%
!! CAAGCTCCTTTGTTGGTAGC+TGG - chr1_1:6698873-6698892 Msa0004280:three_prime_UTR 50.0%
AGCTTGCCAGCTACCAACAA+AGG + chr1_1:6698882-6698901 None:intergenic 50.0%
GCAAGCTCCAAAGGATCCTA+AGG - chr1_1:6698895-6698914 Msa0004280:three_prime_UTR 50.0%
TCGGCTTCCTTAGGATCCTT+TGG + chr1_1:6698905-6698924 None:intergenic 50.0%
AGCATTGCTTCGGCTTCCTT+AGG + chr1_1:6698914-6698933 None:intergenic 50.0%
GCTTGCTCAGCAAGTGAAAG+AGG - chr1_1:6698931-6698950 Msa0004280:three_prime_UTR 50.0%
GCAAGTGAAAGAGGTTCGGA+AGG - chr1_1:6698940-6698959 Msa0004280:three_prime_UTR 50.0%
GTTCGGAAGGAATATGGCGT+TGG - chr1_1:6698953-6698972 Msa0004280:three_prime_UTR 50.0%
TGATGAAGCCGAACTCGAGA+AGG - chr1_1:6699806-6699825 Msa0004280:CDS 50.0%
TTCACGGTGGTTGTTGTGGT+GGG + chr1_1:6698834-6698853 None:intergenic 50.0%
TTTCACGGTGGTTGTTGTGG+TGG + chr1_1:6698835-6698854 None:intergenic 50.0%
! CAACAAAGGAGCTTGGTGAG+AGG + chr1_1:6698868-6698887 None:intergenic 50.0%
CTCTCACCAAGCTCCTTTGT+TGG - chr1_1:6698866-6698885 Msa0004280:three_prime_UTR 50.0%
CAGCTACCAACAAAGGAGCT+TGG + chr1_1:6698875-6698894 None:intergenic 50.0%
!! CAAGCTCCTTTGTTGGTAGC+TGG - chr1_1:6698873-6698892 Msa0004280:three_prime_UTR 50.0%
AGCTTGCCAGCTACCAACAA+AGG + chr1_1:6698882-6698901 None:intergenic 50.0%
GCAAGCTCCAAAGGATCCTA+AGG - chr1_1:6698895-6698914 Msa0004280:three_prime_UTR 50.0%
TCGGCTTCCTTAGGATCCTT+TGG + chr1_1:6698905-6698924 None:intergenic 50.0%
AGCATTGCTTCGGCTTCCTT+AGG + chr1_1:6698914-6698933 None:intergenic 50.0%
GCTTGCTCAGCAAGTGAAAG+AGG - chr1_1:6698931-6698950 Msa0004280:three_prime_UTR 50.0%
GCAAGTGAAAGAGGTTCGGA+AGG - chr1_1:6698940-6698959 Msa0004280:three_prime_UTR 50.0%
GTTCGGAAGGAATATGGCGT+TGG - chr1_1:6698953-6698972 Msa0004280:three_prime_UTR 50.0%
TGATGAAGCCGAACTCGAGA+AGG - chr1_1:6699806-6699825 Msa0004280:CDS 50.0%
TGGTAGCTGGCAAGCTCCAA+AGG - chr1_1:6698886-6698905 Msa0004280:three_prime_UTR 55.0%
GGCTGCAGAACTTAGAGCTC+AGG - chr1_1:6699052-6699071 Msa0004280:CDS 55.0%
TGGTAGCTGGCAAGCTCCAA+AGG - chr1_1:6698886-6698905 Msa0004280:three_prime_UTR 55.0%
GGCTGCAGAACTTAGAGCTC+AGG - chr1_1:6699052-6699071 Msa0004280:CDS 55.0%
! GGCGTTGGAGAAGCAACGCA+AGG - chr1_1:6698968-6698987 Msa0004280:three_prime_UTR 60.0%
GGAGAAGCAACGCAAGGACG+AGG - chr1_1:6698974-6698993 Msa0004280:three_prime_UTR 60.0%
AGCAACGCAAGGACGAGGCT+CGG - chr1_1:6698979-6698998 Msa0004280:three_prime_UTR 60.0%
! GGCGTTGGAGAAGCAACGCA+AGG - chr1_1:6698968-6698987 Msa0004280:three_prime_UTR 60.0%
GGAGAAGCAACGCAAGGACG+AGG - chr1_1:6698974-6698993 Msa0004280:three_prime_UTR 60.0%
AGCAACGCAAGGACGAGGCT+CGG - chr1_1:6698979-6698998 Msa0004280:three_prime_UTR 60.0%
AACGCAAGGACGAGGCTCGG+AGG - chr1_1:6698982-6699001 Msa0004280:three_prime_UTR 65.0%
ACGCAAGGACGAGGCTCGGA+GGG - chr1_1:6698983-6699002 Msa0004280:three_prime_UTR 65.0%
AACGCAAGGACGAGGCTCGG+AGG - chr1_1:6698982-6699001 Msa0004280:three_prime_UTR 65.0%
ACGCAAGGACGAGGCTCGGA+GGG - chr1_1:6698983-6699002 Msa0004280:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 6698717 6700200 6698717 ID=Msa0004280;Name=Msa0004280
chr1_1 mRNA 6698717 6700200 6698717 ID=Msa0004280-mRNA-1;Parent=Msa0004280;Name=Msa0004280-mRNA-1;_AED=0.07;_eAED=0.07;_QI=249|1|1|1|1|1|2|334|106
chr1_1 exon 6698717 6699224 6698717 ID=Msa0004280-mRNA-1:exon:2325;Parent=Msa0004280-mRNA-1
chr1_1 exon 6699805 6700200 6699805 ID=Msa0004280-mRNA-1:exon:2324;Parent=Msa0004280-mRNA-1
chr1_1 five_prime_UTR 6699952 6700200 6699952 ID=Msa0004280-mRNA-1:five_prime_utr;Parent=Msa0004280-mRNA-1
chr1_1 CDS 6699805 6699951 6699805 ID=Msa0004280-mRNA-1:cds;Parent=Msa0004280-mRNA-1
chr1_1 CDS 6699051 6699224 6699051 ID=Msa0004280-mRNA-1:cds;Parent=Msa0004280-mRNA-1
chr1_1 three_prime_UTR 6698717 6699050 6698717 ID=Msa0004280-mRNA-1:three_prime_utr;Parent=Msa0004280-mRNA-1
Gene Sequence

>Msa0004280

ATGGCGTTGGAGAAGCAACGCAAGGACGAGGCTCGGAGGGAATCACTTAGAATCGCTAATGAAGACCGTAAGAAACTTAAGGCTCAGGCTGCAGAACTTAGAGCTCAGGAACGTAACATTGCTAGACAACAGTTTCAAGAAACCCTTTTAAAAGAGAGAGCAGAGAAACTTGAAATTTGGAGAACGCAAGCCAAAAAGCATGAAGAGAAGAAGAAGGCTGAAAAGAAAGAATTATTGCATAAACAAAGTTCACTGTGGGTTGATGAAGCCGAACTCGAGAAGGAAATCACGAATGTTGTAATTGCTACAACATATCTTTGA
Protein sequence

>Msa0004280

MALEKQRKDEARRESLRIANEDRKKLKAQAAELRAQERNIARQQFQETLLKERAEKLEIWRTQAKKHEEKKKAEKKELLHKQSSLWVDEAELEKEITNVVIATTYL*