AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0004780

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0004780 MtrunA17_Chr1g0153171 98.305 236 4 0 1 236 1 236 1.06e-175 482
Msa0004780 MtrunA17_Chr8g0392991 45.255 137 69 1 22 152 22 158 2.24e-29 112
Msa0004780 MtrunA17_Chr7g0238431 71.642 67 19 0 86 152 4 70 5.78e-29 108
Msa0004780 MtrunA17_Chr4g0031341 36.932 176 81 4 90 235 25 200 1.81e-26 103
Msa0004780 MtrunA17_Chr5g0436661 65.152 66 23 0 90 155 33 98 8.85e-26 99.4
Msa0004780 MtrunA17_Chr4g0043891 68.750 64 20 0 89 152 7 70 1.13e-25 100
Msa0004780 MtrunA17_Chr5g0405351 64.062 64 22 1 89 151 29 92 1.20e-22 94.0
Msa0004780 MtrunA17_Chr3g0125111 40.146 137 60 2 22 152 22 142 6.47e-22 88.6
Msa0004780 MtrunA17_Chr8g0385441 76.923 52 12 0 101 152 93 144 2.76e-21 87.0
Msa0004780 MtrunA17_Chr2g0283141 35.577 104 55 1 49 152 26 117 3.87e-14 71.2
Msa0004780 MtrunA17_Chr7g0223471 46.774 62 33 0 90 151 47 108 1.68e-13 68.9
Msa0004780 MtrunA17_Chr3g0144021 39.474 76 46 0 90 165 82 157 3.20e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0004780 AT1G46480.1 52.896 259 91 9 1 236 1 251 1.63e-72 221
Msa0004780 AT1G46480.2 59.067 193 60 7 1 182 1 185 3.05e-63 196
Msa0004780 AT3G18010.1 42.857 147 74 2 6 152 2 138 6.13e-30 114
Msa0004780 AT3G11260.1 56.818 88 33 1 87 169 16 103 1.24e-27 104
Msa0004780 AT5G59340.1 61.333 75 29 0 78 152 2 76 5.75e-27 104
Msa0004780 AT2G28610.1 68.182 66 21 0 87 152 5 70 9.84e-27 103
Msa0004780 AT5G05770.1 63.077 65 24 0 90 154 29 93 1.72e-24 94.4
Msa0004780 AT2G17950.1 51.765 85 40 1 89 172 37 121 1.05e-23 96.7
Msa0004780 AT2G01500.1 66.667 63 19 1 84 144 53 115 2.07e-21 90.1
Msa0004780 AT2G33880.2 38.614 101 52 1 49 149 24 114 4.42e-14 71.2
Msa0004780 AT2G33880.1 38.614 101 52 1 49 149 24 114 4.70e-14 70.9
Msa0004780 AT1G20700.2 47.541 61 32 0 90 150 33 93 2.70e-13 65.9
Msa0004780 AT5G45980.1 46.774 62 33 0 90 151 55 116 4.97e-13 67.8
Msa0004780 AT1G20710.1 38.462 91 44 1 97 187 86 164 6.50e-13 65.9
Msa0004780 AT1G20700.3 47.541 61 32 0 90 150 95 155 9.15e-13 65.5
Msa0004780 AT1G20700.1 47.541 61 32 0 90 150 95 155 1.09e-12 65.5
Msa0004780 AT4G35550.1 47.541 61 32 0 90 150 99 159 1.15e-11 63.2

Find 72 sgRNAs with CRISPR-Local

Find 244 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAATTCACACGTGGATTT+TGG 0.121803 1_1:-7236838 Msa0004780:CDS
ACAAATAAGTTTGGTTAATT+TGG 0.133382 1_1:+7235295 None:intergenic
AAGCAAATTAAAGCACATTT+TGG 0.180958 1_1:+7235427 None:intergenic
TCTCGCGTGCTTTGTGGTTT+TGG 0.189639 1_1:+7236012 None:intergenic
AAACTCCTTCATCTGCCTTC+TGG 0.237007 1_1:+7235479 None:intergenic
AACTCCTTCATCTGCCTTCT+GGG 0.285945 1_1:+7235480 None:intergenic
CAACTTTGGACACCCCAAAA+AGG 0.296385 1_1:-7235893 Msa0004780:CDS
ACTAATTCTAGGAGCACTTT+CGG 0.300053 1_1:+7236682 None:intergenic
CTAATTCTAGGAGCACTTTC+GGG 0.314910 1_1:+7236683 None:intergenic
TGTTCTATTTGTTGTGCATT+TGG 0.328080 1_1:+7236095 None:intergenic
ACAAGAACAAATAGGAATAT+TGG 0.328145 1_1:-7236147 Msa0004780:CDS
GAGGAAGAAAGACTAATTCT+AGG 0.342909 1_1:+7236671 None:intergenic
GGATTCCACCTTGTCCCTCC+TGG 0.366142 1_1:+7236173 None:intergenic
ATCTGCCTTCTGGGTGTAAT+GGG 0.376276 1_1:+7235489 None:intergenic
CTTTAGTACTATTACAACTT+TGG 0.384910 1_1:-7235907 Msa0004780:CDS
TTTGCCTCTCGCGTGCTTTG+TGG 0.398309 1_1:+7236006 None:intergenic
CATCTGCCTTCTGGGTGTAA+TGG 0.398760 1_1:+7235488 None:intergenic
ATTCCTATTTGTTCTTGTGT+TGG 0.415008 1_1:+7236152 None:intergenic
TTTCTTCTTTCATTTACATT+GGG 0.415761 1_1:-7235394 Msa0004780:three_prime_UTR
AATCCAACACAAGAACAAAT+AGG 0.426122 1_1:-7236155 Msa0004780:CDS
GTAATGTTATTCCATGACTT+TGG 0.426731 1_1:-7235320 Msa0004780:three_prime_UTR
TTCTCTTCCAAGTACTCAAA+TGG 0.427494 1_1:+7235560 None:intergenic
ATTTCTTCTTTCATTTACAT+TGG 0.443688 1_1:-7235395 Msa0004780:three_prime_UTR
TAGGTTGAAACGCATATTCC+AGG 0.450427 1_1:-7236191 Msa0004780:CDS
TCCACCTTCACCGCAAAGCC+AGG 0.461999 1_1:-7236630 Msa0004780:intron
TTTGTTGATTTCAGCGTAGT+AGG 0.464270 1_1:-7235258 Msa0004780:three_prime_UTR
GAGTTGGCCATTTGAGTACT+TGG 0.464745 1_1:-7235567 Msa0004780:CDS
GGTGTTAGAAGTAACATTAT+CGG 0.465029 1_1:+7236742 None:intergenic
CAGCGTAGTAGGTGTGACTT+TGG 0.468785 1_1:-7235247 Msa0004780:three_prime_UTR
CACAGACACAAGTGTGGTGG+TGG 0.469900 1_1:+7235938 None:intergenic
CAAAGTTGTAATAGTACTAA+AGG 0.472738 1_1:+7235908 None:intergenic
AAGATAAGATTAGTAGAAGT+AGG 0.473815 1_1:+7235132 None:intergenic
AGCAAGTACGGTAAAATTGA+AGG 0.477134 1_1:-7236056 Msa0004780:CDS
CAACTCCTACTCTTCTTCAA+TGG 0.501838 1_1:+7235584 None:intergenic
AGTGTGGTGGTGGGAGTTCG+AGG 0.518160 1_1:+7235948 None:intergenic
CATGAACCTTCCCTCACACT+TGG 0.520457 1_1:-7236812 Msa0004780:CDS
TGAACCTGGCTTTGCGGTGA+AGG 0.521265 1_1:+7236626 None:intergenic
GTTGTAATAGTACTAAAGGT+GGG 0.528602 1_1:+7235912 None:intergenic
TGAGATCAAAAGAAAGAGAA+GGG 0.531578 1_1:+7236720 None:intergenic
TTAATTTGGCTCCAAAGTCA+TGG 0.533407 1_1:+7235309 None:intergenic
AAGCAGAAACGTAACAGTCT+TGG 0.535210 1_1:-7235984 Msa0004780:CDS
AGTATATGAACCTGGCTTTG+CGG 0.544738 1_1:+7236620 None:intergenic
ATAGTCCATTGAAGAAGAGT+AGG 0.545832 1_1:-7235589 Msa0004780:CDS
ACAGTTCAACTTAGCAAGTA+CGG 0.553808 1_1:-7236068 Msa0004780:CDS
ATCTTTCTTGTTATCATCAG+AGG 0.556963 1_1:+7236652 None:intergenic
CGCATATTCCAGGAGGGACA+AGG 0.565252 1_1:-7236181 Msa0004780:CDS
TACACCCAGAAGGCAGATGA+AGG 0.566613 1_1:-7235484 Msa0004780:exon
GTGGGAGTTCGAGGACTATG+AGG 0.567688 1_1:+7235957 None:intergenic
TTGGTCAATGTGCAAAGTAG+AGG 0.568050 1_1:-7235534 Msa0004780:CDS
TTGAGATCAAAAGAAAGAGA+AGG 0.574637 1_1:+7236719 None:intergenic
AGTTGTAATAGTACTAAAGG+TGG 0.581733 1_1:+7235911 None:intergenic
AACGCTTGCAACCAAGTGTG+AGG 0.582297 1_1:+7236801 None:intergenic
GCAAGTACGGTAAAATTGAA+GGG 0.588273 1_1:-7236055 Msa0004780:CDS
CATTGAAGAAGAGTAGGAGT+TGG 0.590517 1_1:-7235583 Msa0004780:CDS
ACCTGGCTTTGCGGTGAAGG+TGG 0.590968 1_1:+7236629 None:intergenic
TGTAATAGTACTAAAGGTGG+GGG 0.603492 1_1:+7235914 None:intergenic
TTGTAATAGTACTAAAGGTG+GGG 0.606911 1_1:+7235913 None:intergenic
TTGGAGATGTTATACAGAGG+TGG 0.611718 1_1:-7236128 Msa0004780:CDS
GAGTTCGAGGACTATGAGGA+AGG 0.633348 1_1:+7235961 None:intergenic
GATTAGTAGAAGTAGGTGCG+TGG 0.641260 1_1:+7235139 None:intergenic
TATTTCTTCATCAGACCGTG+TGG 0.650611 1_1:-7236898 Msa0004780:five_prime_UTR
ACGCTTGCAACCAAGTGTGA+GGG 0.656705 1_1:+7236802 None:intergenic
TTGAAACGCATATTCCAGGA+GGG 0.657126 1_1:-7236187 Msa0004780:CDS
ACAGACACAAGTGTGGTGGT+GGG 0.659851 1_1:+7235939 None:intergenic
AAAACCACAAAGCACGCGAG+AGG 0.660578 1_1:-7236010 Msa0004780:CDS
GGACACAGACACAAGTGTGG+TGG 0.666759 1_1:+7235935 None:intergenic
GAGATCAAAAGAAAGAGAAG+GGG 0.671823 1_1:+7236721 None:intergenic
TTGCAACCAAGTGTGAGGGA+AGG 0.674259 1_1:+7236806 None:intergenic
GTTGAAACGCATATTCCAGG+AGG 0.708848 1_1:-7236188 Msa0004780:CDS
ATATTGGAGATGTTATACAG+AGG 0.712720 1_1:-7236131 Msa0004780:CDS
AAAGTGCATCAATTCACACG+TGG 0.742454 1_1:-7236845 Msa0004780:CDS
ATATTCCAGGAGGGACAAGG+TGG 0.756182 1_1:-7236178 Msa0004780:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATAAAAAAAAAAAAATTA+CGG + chr1_1:7236217-7236236 None:intergenic 0.0%
!!! ATTTTTTTTTTTTTATTAAA+AGG - chr1_1:7236219-7236238 Msa0004780:intron 0.0%
!! TAATAAAAAAAAAAAAATTA+CGG + chr1_1:7236217-7236236 None:intergenic 0.0%
!!! ATTTTTTTTTTTTTATTAAA+AGG - chr1_1:7236219-7236238 Msa0004780:intron 0.0%
!!! ATAATACTAATAAAGTATTT+TGG - chr1_1:7235556-7235575 Msa0004780:CDS 10.0%
!!! ATAATACTAATAAAGTATTT+TGG - chr1_1:7235556-7235575 Msa0004780:CDS 10.0%
!! ACAGACTTAATTAAAAAAAA+TGG + chr1_1:7235401-7235420 None:intergenic 15.0%
!!! TTTTTTATTAAAAGGAAAGT+AGG - chr1_1:7236227-7236246 Msa0004780:intron 15.0%
!! AAATCAACAAATGAAAAAAA+AGG + chr1_1:7236707-7236726 None:intergenic 15.0%
!! ACAGACTTAATTAAAAAAAA+TGG + chr1_1:7235401-7235420 None:intergenic 15.0%
!!! TTTTTTATTAAAAGGAAAGT+AGG - chr1_1:7236227-7236246 Msa0004780:intron 15.0%
!! AAATCAACAAATGAAAAAAA+AGG + chr1_1:7236707-7236726 None:intergenic 15.0%
!!! GTTTAATATAATTTTCCACA+CGG + chr1_1:7235094-7235113 None:intergenic 20.0%
!!! GTGTTATTAGAAGAAATTTT+AGG + chr1_1:7235213-7235232 None:intergenic 20.0%
!! ATTAACTTCATGTAACATAT+AGG - chr1_1:7235513-7235532 Msa0004780:CDS 20.0%
!! TTAACTTCATGTAACATATA+GGG - chr1_1:7235514-7235533 Msa0004780:CDS 20.0%
!!! ATATAGATACATCTGAAATT+CGG + chr1_1:7235601-7235620 None:intergenic 20.0%
!! TCTATTATTTGTGTATGTAT+AGG - chr1_1:7235764-7235783 Msa0004780:intron 20.0%
!!! CGACAATATAAAAGATTTTA+AGG + chr1_1:7236175-7236194 None:intergenic 20.0%
!!! TTGTTCATGGTTTTAAATTA+CGG + chr1_1:7236254-7236273 None:intergenic 20.0%
!! AATTAGTATACTATTGTTCA+TGG + chr1_1:7236267-7236286 None:intergenic 20.0%
!! AATACTATAATCTTGTTACT+AGG - chr1_1:7236289-7236308 Msa0004780:intron 20.0%
!!! TCGAATATGTTACATTTTTT+TGG - chr1_1:7236330-7236349 Msa0004780:intron 20.0%
!! ATTTCTTCTTTCATTTACAT+TGG - chr1_1:7236579-7236598 Msa0004780:intron 20.0%
!! TTTCTTCTTTCATTTACATT+GGG - chr1_1:7236580-7236599 Msa0004780:intron 20.0%
!! ACAAATAAGTTTGGTTAATT+TGG + chr1_1:7236682-7236701 None:intergenic 20.0%
!!! ATACTATACTCTCATATTTT+AGG - chr1_1:7236880-7236899 Msa0004780:five_prime_UTR 20.0%
!!! GTTTAATATAATTTTCCACA+CGG + chr1_1:7235094-7235113 None:intergenic 20.0%
!!! GTGTTATTAGAAGAAATTTT+AGG + chr1_1:7235213-7235232 None:intergenic 20.0%
!! ATTAACTTCATGTAACATAT+AGG - chr1_1:7235513-7235532 Msa0004780:CDS 20.0%
!! TTAACTTCATGTAACATATA+GGG - chr1_1:7235514-7235533 Msa0004780:CDS 20.0%
!!! ATATAGATACATCTGAAATT+CGG + chr1_1:7235601-7235620 None:intergenic 20.0%
!! TCTATTATTTGTGTATGTAT+AGG - chr1_1:7235764-7235783 Msa0004780:intron 20.0%
!!! CGACAATATAAAAGATTTTA+AGG + chr1_1:7236175-7236194 None:intergenic 20.0%
!!! TTGTTCATGGTTTTAAATTA+CGG + chr1_1:7236254-7236273 None:intergenic 20.0%
!! AATTAGTATACTATTGTTCA+TGG + chr1_1:7236267-7236286 None:intergenic 20.0%
!! AATACTATAATCTTGTTACT+AGG - chr1_1:7236289-7236308 Msa0004780:intron 20.0%
!!! TCGAATATGTTACATTTTTT+TGG - chr1_1:7236330-7236349 Msa0004780:intron 20.0%
!! ATTTCTTCTTTCATTTACAT+TGG - chr1_1:7236579-7236598 Msa0004780:intron 20.0%
!! TTTCTTCTTTCATTTACATT+GGG - chr1_1:7236580-7236599 Msa0004780:intron 20.0%
!! ACAAATAAGTTTGGTTAATT+TGG + chr1_1:7236682-7236701 None:intergenic 20.0%
!!! ATACTATACTCTCATATTTT+AGG - chr1_1:7236880-7236899 Msa0004780:five_prime_UTR 20.0%
!!! ACTAATAAAGTATTTTGGTC+CGG - chr1_1:7235561-7235580 Msa0004780:CDS 25.0%
!!! TCGTTTAAAAGCTTTTGATT+TGG - chr1_1:7235714-7235733 Msa0004780:intron 25.0%
! ACAAGAACAAATAGGAATAT+TGG - chr1_1:7235827-7235846 Msa0004780:intron 25.0%
! CAAAGTTGTAATAGTACTAA+AGG + chr1_1:7236069-7236088 None:intergenic 25.0%
! CTTTAGTACTATTACAACTT+TGG - chr1_1:7236067-7236086 Msa0004780:CDS 25.0%
!! AATATGAACATACCCTTTTT+GGG + chr1_1:7236097-7236116 None:intergenic 25.0%
! TCAACTTAAGCTATATATTG+TGG - chr1_1:7236127-7236146 Msa0004780:CDS 25.0%
!!! TATGTTACATTTTTTTGGAC+AGG - chr1_1:7236335-7236354 Msa0004780:intron 25.0%
!! AAGCAAATTAAAGCACATTT+TGG + chr1_1:7236550-7236569 None:intergenic 25.0%
! AAAAAGGTGACAAATAAGTT+TGG + chr1_1:7236691-7236710 None:intergenic 25.0%
! AAGATAAGATTAGTAGAAGT+AGG + chr1_1:7236845-7236864 None:intergenic 25.0%
!!! ACTAATAAAGTATTTTGGTC+CGG - chr1_1:7235561-7235580 Msa0004780:CDS 25.0%
!!! TCGTTTAAAAGCTTTTGATT+TGG - chr1_1:7235714-7235733 Msa0004780:intron 25.0%
! ACAAGAACAAATAGGAATAT+TGG - chr1_1:7235827-7235846 Msa0004780:intron 25.0%
! CAAAGTTGTAATAGTACTAA+AGG + chr1_1:7236069-7236088 None:intergenic 25.0%
! CTTTAGTACTATTACAACTT+TGG - chr1_1:7236067-7236086 Msa0004780:CDS 25.0%
!! AATATGAACATACCCTTTTT+GGG + chr1_1:7236097-7236116 None:intergenic 25.0%
! TCAACTTAAGCTATATATTG+TGG - chr1_1:7236127-7236146 Msa0004780:CDS 25.0%
!!! TATGTTACATTTTTTTGGAC+AGG - chr1_1:7236335-7236354 Msa0004780:intron 25.0%
!! AAGCAAATTAAAGCACATTT+TGG + chr1_1:7236550-7236569 None:intergenic 25.0%
! AAAAAGGTGACAAATAAGTT+TGG + chr1_1:7236691-7236710 None:intergenic 25.0%
! AAGATAAGATTAGTAGAAGT+AGG + chr1_1:7236845-7236864 None:intergenic 25.0%
GGTGTTAGAAGTAACATTAT+CGG + chr1_1:7235235-7235254 None:intergenic 30.0%
TGAGATCAAAAGAAAGAGAA+GGG + chr1_1:7235257-7235276 None:intergenic 30.0%
TTGAGATCAAAAGAAAGAGA+AGG + chr1_1:7235258-7235277 None:intergenic 30.0%
! ATCTTTCTTGTTATCATCAG+AGG + chr1_1:7235325-7235344 None:intergenic 30.0%
!!! CTAATAAAGTATTTTGGTCC+GGG - chr1_1:7235562-7235581 Msa0004780:CDS 30.0%
AATCCAACACAAGAACAAAT+AGG - chr1_1:7235819-7235838 Msa0004780:intron 30.0%
ATTCCTATTTGTTCTTGTGT+TGG + chr1_1:7235825-7235844 None:intergenic 30.0%
ATATTGGAGATGTTATACAG+AGG - chr1_1:7235843-7235862 Msa0004780:intron 30.0%
!! TGTTCTATTTGTTGTGCATT+TGG + chr1_1:7235882-7235901 None:intergenic 30.0%
AAGGGAAAAATGTGTTCTAT+TGG - chr1_1:7235937-7235956 Msa0004780:CDS 30.0%
TTGTAATAGTACTAAAGGTG+GGG + chr1_1:7236064-7236083 None:intergenic 30.0%
GTTGTAATAGTACTAAAGGT+GGG + chr1_1:7236065-7236084 None:intergenic 30.0%
AGTTGTAATAGTACTAAAGG+TGG + chr1_1:7236066-7236085 None:intergenic 30.0%
! ATATGAACATACCCTTTTTG+GGG + chr1_1:7236096-7236115 None:intergenic 30.0%
! GAATATGAACATACCCTTTT+TGG + chr1_1:7236098-7236117 None:intergenic 30.0%
AGTACTTGGAAGAGAAAAAT+TGG - chr1_1:7236421-7236440 Msa0004780:intron 30.0%
GTAATGTTATTCCATGACTT+TGG - chr1_1:7236654-7236673 Msa0004780:CDS 30.0%
AAGCTTATGTCGTCTAAAAA+AGG + chr1_1:7236764-7236783 None:intergenic 30.0%
!!! TCGAAAGCTTTTAGGTTAAA+AGG - chr1_1:7236789-7236808 Msa0004780:CDS 30.0%
GGTGTTAGAAGTAACATTAT+CGG + chr1_1:7235235-7235254 None:intergenic 30.0%
TGAGATCAAAAGAAAGAGAA+GGG + chr1_1:7235257-7235276 None:intergenic 30.0%
TTGAGATCAAAAGAAAGAGA+AGG + chr1_1:7235258-7235277 None:intergenic 30.0%
! ATCTTTCTTGTTATCATCAG+AGG + chr1_1:7235325-7235344 None:intergenic 30.0%
!!! CTAATAAAGTATTTTGGTCC+GGG - chr1_1:7235562-7235581 Msa0004780:CDS 30.0%
AATCCAACACAAGAACAAAT+AGG - chr1_1:7235819-7235838 Msa0004780:intron 30.0%
ATTCCTATTTGTTCTTGTGT+TGG + chr1_1:7235825-7235844 None:intergenic 30.0%
ATATTGGAGATGTTATACAG+AGG - chr1_1:7235843-7235862 Msa0004780:intron 30.0%
!! TGTTCTATTTGTTGTGCATT+TGG + chr1_1:7235882-7235901 None:intergenic 30.0%
AAGGGAAAAATGTGTTCTAT+TGG - chr1_1:7235937-7235956 Msa0004780:CDS 30.0%
TTGTAATAGTACTAAAGGTG+GGG + chr1_1:7236064-7236083 None:intergenic 30.0%
GTTGTAATAGTACTAAAGGT+GGG + chr1_1:7236065-7236084 None:intergenic 30.0%
AGTTGTAATAGTACTAAAGG+TGG + chr1_1:7236066-7236085 None:intergenic 30.0%
! ATATGAACATACCCTTTTTG+GGG + chr1_1:7236096-7236115 None:intergenic 30.0%
! GAATATGAACATACCCTTTT+TGG + chr1_1:7236098-7236117 None:intergenic 30.0%
AGTACTTGGAAGAGAAAAAT+TGG - chr1_1:7236421-7236440 Msa0004780:intron 30.0%
GTAATGTTATTCCATGACTT+TGG - chr1_1:7236654-7236673 Msa0004780:CDS 30.0%
AAGCTTATGTCGTCTAAAAA+AGG + chr1_1:7236764-7236783 None:intergenic 30.0%
!!! TCGAAAGCTTTTAGGTTAAA+AGG - chr1_1:7236789-7236808 Msa0004780:CDS 30.0%
! ATCAATTCACACGTGGATTT+TGG - chr1_1:7235136-7235155 Msa0004780:three_prime_UTR 35.0%
! TCAATTCACACGTGGATTTT+GGG - chr1_1:7235137-7235156 Msa0004780:three_prime_UTR 35.0%
!! GAAGAAATTTTAGGTGCAAG+AGG + chr1_1:7235204-7235223 None:intergenic 35.0%
GAGATCAAAAGAAAGAGAAG+GGG + chr1_1:7235256-7235275 None:intergenic 35.0%
!! ACTAATTCTAGGAGCACTTT+CGG + chr1_1:7235295-7235314 None:intergenic 35.0%
GAGGAAGAAAGACTAATTCT+AGG + chr1_1:7235306-7235325 None:intergenic 35.0%
TAGAGCTTAGTATATGAACC+TGG + chr1_1:7235365-7235384 None:intergenic 35.0%
! TTCGGTTCAACAATTTTTCC+CGG + chr1_1:7235583-7235602 None:intergenic 35.0%
ACAGTTCAACTTAGCAAGTA+CGG - chr1_1:7235906-7235925 Msa0004780:CDS 35.0%
AGCAAGTACGGTAAAATTGA+AGG - chr1_1:7235918-7235937 Msa0004780:CDS 35.0%
GCAAGTACGGTAAAATTGAA+GGG - chr1_1:7235919-7235938 Msa0004780:CDS 35.0%
TGTAATAGTACTAAAGGTGG+GGG + chr1_1:7236063-7236082 None:intergenic 35.0%
ATAGTCCATTGAAGAAGAGT+AGG - chr1_1:7236385-7236404 Msa0004780:intron 35.0%
TTCTCTTCCAAGTACTCAAA+TGG + chr1_1:7236417-7236436 None:intergenic 35.0%
! TTAATTTGGCTCCAAAGTCA+TGG + chr1_1:7236668-7236687 None:intergenic 35.0%
!! TTTGTTGATTTCAGCGTAGT+AGG - chr1_1:7236716-7236735 Msa0004780:CDS 35.0%
! CTTGTGAATCGAAAGCTTTT+AGG - chr1_1:7236781-7236800 Msa0004780:CDS 35.0%
! ATCAATTCACACGTGGATTT+TGG - chr1_1:7235136-7235155 Msa0004780:three_prime_UTR 35.0%
! TCAATTCACACGTGGATTTT+GGG - chr1_1:7235137-7235156 Msa0004780:three_prime_UTR 35.0%
!! GAAGAAATTTTAGGTGCAAG+AGG + chr1_1:7235204-7235223 None:intergenic 35.0%
GAGATCAAAAGAAAGAGAAG+GGG + chr1_1:7235256-7235275 None:intergenic 35.0%
!! ACTAATTCTAGGAGCACTTT+CGG + chr1_1:7235295-7235314 None:intergenic 35.0%
GAGGAAGAAAGACTAATTCT+AGG + chr1_1:7235306-7235325 None:intergenic 35.0%
TAGAGCTTAGTATATGAACC+TGG + chr1_1:7235365-7235384 None:intergenic 35.0%
! TTCGGTTCAACAATTTTTCC+CGG + chr1_1:7235583-7235602 None:intergenic 35.0%
ACAGTTCAACTTAGCAAGTA+CGG - chr1_1:7235906-7235925 Msa0004780:CDS 35.0%
AGCAAGTACGGTAAAATTGA+AGG - chr1_1:7235918-7235937 Msa0004780:CDS 35.0%
GCAAGTACGGTAAAATTGAA+GGG - chr1_1:7235919-7235938 Msa0004780:CDS 35.0%
TGTAATAGTACTAAAGGTGG+GGG + chr1_1:7236063-7236082 None:intergenic 35.0%
ATAGTCCATTGAAGAAGAGT+AGG - chr1_1:7236385-7236404 Msa0004780:intron 35.0%
TTCTCTTCCAAGTACTCAAA+TGG + chr1_1:7236417-7236436 None:intergenic 35.0%
! TTAATTTGGCTCCAAAGTCA+TGG + chr1_1:7236668-7236687 None:intergenic 35.0%
!! TTTGTTGATTTCAGCGTAGT+AGG - chr1_1:7236716-7236735 Msa0004780:CDS 35.0%
! CTTGTGAATCGAAAGCTTTT+AGG - chr1_1:7236781-7236800 Msa0004780:CDS 35.0%
TATTTCTTCATCAGACCGTG+TGG - chr1_1:7235076-7235095 Msa0004780:three_prime_UTR 40.0%
AAAGTGCATCAATTCACACG+TGG - chr1_1:7235129-7235148 Msa0004780:three_prime_UTR 40.0%
!! CTAATTCTAGGAGCACTTTC+GGG + chr1_1:7235294-7235313 None:intergenic 40.0%
!! AGTATATGAACCTGGCTTTG+CGG + chr1_1:7235357-7235376 None:intergenic 40.0%
AATTATACCACCCTACTGCA+TGG + chr1_1:7235428-7235447 None:intergenic 40.0%
CGAGTCTCATATGGAAAAAG+AGG + chr1_1:7235656-7235675 None:intergenic 40.0%
AGTAACTCTCGAGTCTCATA+TGG + chr1_1:7235665-7235684 None:intergenic 40.0%
! TAGGTTGAAACGCATATTCC+AGG - chr1_1:7235783-7235802 Msa0004780:intron 40.0%
TTGAAACGCATATTCCAGGA+GGG - chr1_1:7235787-7235806 Msa0004780:intron 40.0%
TTGGAGATGTTATACAGAGG+TGG - chr1_1:7235846-7235865 Msa0004780:intron 40.0%
AAGCAGAAACGTAACAGTCT+TGG - chr1_1:7235990-7236009 Msa0004780:CDS 40.0%
AACTTTGGACACCCCAAAAA+GGG - chr1_1:7236082-7236101 Msa0004780:CDS 40.0%
! TTTTGGACAGGTGCAAGTAA+TGG - chr1_1:7236347-7236366 Msa0004780:intron 40.0%
CAACTCCTACTCTTCTTCAA+TGG + chr1_1:7236393-7236412 None:intergenic 40.0%
CATTGAAGAAGAGTAGGAGT+TGG - chr1_1:7236391-7236410 Msa0004780:intron 40.0%
TTGGTCAATGTGCAAAGTAG+AGG - chr1_1:7236440-7236459 Msa0004780:intron 40.0%
TATTTCTTCATCAGACCGTG+TGG - chr1_1:7235076-7235095 Msa0004780:three_prime_UTR 40.0%
AAAGTGCATCAATTCACACG+TGG - chr1_1:7235129-7235148 Msa0004780:three_prime_UTR 40.0%
!! CTAATTCTAGGAGCACTTTC+GGG + chr1_1:7235294-7235313 None:intergenic 40.0%
!! AGTATATGAACCTGGCTTTG+CGG + chr1_1:7235357-7235376 None:intergenic 40.0%
AATTATACCACCCTACTGCA+TGG + chr1_1:7235428-7235447 None:intergenic 40.0%
CGAGTCTCATATGGAAAAAG+AGG + chr1_1:7235656-7235675 None:intergenic 40.0%
AGTAACTCTCGAGTCTCATA+TGG + chr1_1:7235665-7235684 None:intergenic 40.0%
! TAGGTTGAAACGCATATTCC+AGG - chr1_1:7235783-7235802 Msa0004780:intron 40.0%
TTGAAACGCATATTCCAGGA+GGG - chr1_1:7235787-7235806 Msa0004780:intron 40.0%
TTGGAGATGTTATACAGAGG+TGG - chr1_1:7235846-7235865 Msa0004780:intron 40.0%
AAGCAGAAACGTAACAGTCT+TGG - chr1_1:7235990-7236009 Msa0004780:CDS 40.0%
AACTTTGGACACCCCAAAAA+GGG - chr1_1:7236082-7236101 Msa0004780:CDS 40.0%
! TTTTGGACAGGTGCAAGTAA+TGG - chr1_1:7236347-7236366 Msa0004780:intron 40.0%
CAACTCCTACTCTTCTTCAA+TGG + chr1_1:7236393-7236412 None:intergenic 40.0%
CATTGAAGAAGAGTAGGAGT+TGG - chr1_1:7236391-7236410 Msa0004780:intron 40.0%
TTGGTCAATGTGCAAAGTAG+AGG - chr1_1:7236440-7236459 Msa0004780:intron 40.0%
GTTGAAACGCATATTCCAGG+AGG - chr1_1:7235786-7235805 Msa0004780:intron 45.0%
CAACTTTGGACACCCCAAAA+AGG - chr1_1:7236081-7236100 Msa0004780:CDS 45.0%
ATTGTGGTTCCGAAATGTCG+TGG - chr1_1:7236143-7236162 Msa0004780:CDS 45.0%
TGTCGACGCAACTATAGTCT+TGG - chr1_1:7236190-7236209 Msa0004780:CDS 45.0%
GAGTTGGCCATTTGAGTACT+TGG - chr1_1:7236407-7236426 Msa0004780:intron 45.0%
! CTTTTCCCATTACACCCAGA+AGG - chr1_1:7236480-7236499 Msa0004780:intron 45.0%
ATCTGCCTTCTGGGTGTAAT+GGG + chr1_1:7236488-7236507 None:intergenic 45.0%
AACTCCTTCATCTGCCTTCT+GGG + chr1_1:7236497-7236516 None:intergenic 45.0%
AAACTCCTTCATCTGCCTTC+TGG + chr1_1:7236498-7236517 None:intergenic 45.0%
GATTAGTAGAAGTAGGTGCG+TGG + chr1_1:7236838-7236857 None:intergenic 45.0%
GTTGAAACGCATATTCCAGG+AGG - chr1_1:7235786-7235805 Msa0004780:intron 45.0%
CAACTTTGGACACCCCAAAA+AGG - chr1_1:7236081-7236100 Msa0004780:CDS 45.0%
ATTGTGGTTCCGAAATGTCG+TGG - chr1_1:7236143-7236162 Msa0004780:CDS 45.0%
TGTCGACGCAACTATAGTCT+TGG - chr1_1:7236190-7236209 Msa0004780:CDS 45.0%
GAGTTGGCCATTTGAGTACT+TGG - chr1_1:7236407-7236426 Msa0004780:intron 45.0%
! CTTTTCCCATTACACCCAGA+AGG - chr1_1:7236480-7236499 Msa0004780:intron 45.0%
ATCTGCCTTCTGGGTGTAAT+GGG + chr1_1:7236488-7236507 None:intergenic 45.0%
AACTCCTTCATCTGCCTTCT+GGG + chr1_1:7236497-7236516 None:intergenic 45.0%
AAACTCCTTCATCTGCCTTC+TGG + chr1_1:7236498-7236517 None:intergenic 45.0%
GATTAGTAGAAGTAGGTGCG+TGG + chr1_1:7236838-7236857 None:intergenic 45.0%
CATGAACCTTCCCTCACACT+TGG - chr1_1:7235162-7235181 Msa0004780:three_prime_UTR 50.0%
TTGCAACCAAGTGTGAGGGA+AGG + chr1_1:7235171-7235190 None:intergenic 50.0%
ACGCTTGCAACCAAGTGTGA+GGG + chr1_1:7235175-7235194 None:intergenic 50.0%
AACGCTTGCAACCAAGTGTG+AGG + chr1_1:7235176-7235195 None:intergenic 50.0%
AGTCTGTCCATCCATGCAGT+AGG - chr1_1:7235414-7235433 Msa0004780:three_prime_UTR 50.0%
GTCTGTCCATCCATGCAGTA+GGG - chr1_1:7235415-7235434 Msa0004780:three_prime_UTR 50.0%
ATACCACCCTACTGCATGGA+TGG + chr1_1:7235424-7235443 None:intergenic 50.0%
ATATTCCAGGAGGGACAAGG+TGG - chr1_1:7235796-7235815 Msa0004780:intron 50.0%
! TCTCGCGTGCTTTGTGGTTT+TGG + chr1_1:7235965-7235984 None:intergenic 50.0%
AAAACCACAAAGCACGCGAG+AGG - chr1_1:7235964-7235983 Msa0004780:CDS 50.0%
GAGTTCGAGGACTATGAGGA+AGG + chr1_1:7236016-7236035 None:intergenic 50.0%
ACAGACACAAGTGTGGTGGT+GGG + chr1_1:7236038-7236057 None:intergenic 50.0%
AGGTCTCTGCCACGACATTT+CGG + chr1_1:7236155-7236174 None:intergenic 50.0%
CATCTGCCTTCTGGGTGTAA+TGG + chr1_1:7236489-7236508 None:intergenic 50.0%
TACACCCAGAAGGCAGATGA+AGG - chr1_1:7236490-7236509 Msa0004780:intron 50.0%
CAGCGTAGTAGGTGTGACTT+TGG - chr1_1:7236727-7236746 Msa0004780:CDS 50.0%
CATGAACCTTCCCTCACACT+TGG - chr1_1:7235162-7235181 Msa0004780:three_prime_UTR 50.0%
TTGCAACCAAGTGTGAGGGA+AGG + chr1_1:7235171-7235190 None:intergenic 50.0%
ACGCTTGCAACCAAGTGTGA+GGG + chr1_1:7235175-7235194 None:intergenic 50.0%
AACGCTTGCAACCAAGTGTG+AGG + chr1_1:7235176-7235195 None:intergenic 50.0%
AGTCTGTCCATCCATGCAGT+AGG - chr1_1:7235414-7235433 Msa0004780:three_prime_UTR 50.0%
GTCTGTCCATCCATGCAGTA+GGG - chr1_1:7235415-7235434 Msa0004780:three_prime_UTR 50.0%
ATACCACCCTACTGCATGGA+TGG + chr1_1:7235424-7235443 None:intergenic 50.0%
ATATTCCAGGAGGGACAAGG+TGG - chr1_1:7235796-7235815 Msa0004780:intron 50.0%
! TCTCGCGTGCTTTGTGGTTT+TGG + chr1_1:7235965-7235984 None:intergenic 50.0%
AAAACCACAAAGCACGCGAG+AGG - chr1_1:7235964-7235983 Msa0004780:CDS 50.0%
GAGTTCGAGGACTATGAGGA+AGG + chr1_1:7236016-7236035 None:intergenic 50.0%
ACAGACACAAGTGTGGTGGT+GGG + chr1_1:7236038-7236057 None:intergenic 50.0%
AGGTCTCTGCCACGACATTT+CGG + chr1_1:7236155-7236174 None:intergenic 50.0%
CATCTGCCTTCTGGGTGTAA+TGG + chr1_1:7236489-7236508 None:intergenic 50.0%
TACACCCAGAAGGCAGATGA+AGG - chr1_1:7236490-7236509 Msa0004780:intron 50.0%
CAGCGTAGTAGGTGTGACTT+TGG - chr1_1:7236727-7236746 Msa0004780:CDS 50.0%
!! TGAACCTGGCTTTGCGGTGA+AGG + chr1_1:7235351-7235370 None:intergenic 55.0%
TGTCCATCCATGCAGTAGGG+TGG - chr1_1:7235418-7235437 Msa0004780:three_prime_UTR 55.0%
CGCATATTCCAGGAGGGACA+AGG - chr1_1:7235793-7235812 Msa0004780:intron 55.0%
TTTGCCTCTCGCGTGCTTTG+TGG + chr1_1:7235971-7235990 None:intergenic 55.0%
GTGGGAGTTCGAGGACTATG+AGG + chr1_1:7236020-7236039 None:intergenic 55.0%
CACAGACACAAGTGTGGTGG+TGG + chr1_1:7236039-7236058 None:intergenic 55.0%
GGACACAGACACAAGTGTGG+TGG + chr1_1:7236042-7236061 None:intergenic 55.0%
!! TGAACCTGGCTTTGCGGTGA+AGG + chr1_1:7235351-7235370 None:intergenic 55.0%
TGTCCATCCATGCAGTAGGG+TGG - chr1_1:7235418-7235437 Msa0004780:three_prime_UTR 55.0%
CGCATATTCCAGGAGGGACA+AGG - chr1_1:7235793-7235812 Msa0004780:intron 55.0%
TTTGCCTCTCGCGTGCTTTG+TGG + chr1_1:7235971-7235990 None:intergenic 55.0%
GTGGGAGTTCGAGGACTATG+AGG + chr1_1:7236020-7236039 None:intergenic 55.0%
CACAGACACAAGTGTGGTGG+TGG + chr1_1:7236039-7236058 None:intergenic 55.0%
GGACACAGACACAAGTGTGG+TGG + chr1_1:7236042-7236061 None:intergenic 55.0%
TCCACCTTCACCGCAAAGCC+AGG - chr1_1:7235344-7235363 Msa0004780:three_prime_UTR 60.0%
!! ACCTGGCTTTGCGGTGAAGG+TGG + chr1_1:7235348-7235367 None:intergenic 60.0%
GGATTCCACCTTGTCCCTCC+TGG + chr1_1:7235804-7235823 None:intergenic 60.0%
!! AGTGTGGTGGTGGGAGTTCG+AGG + chr1_1:7236029-7236048 None:intergenic 60.0%
GGGGGACACAGACACAAGTG+TGG + chr1_1:7236045-7236064 None:intergenic 60.0%
TCCACCTTCACCGCAAAGCC+AGG - chr1_1:7235344-7235363 Msa0004780:three_prime_UTR 60.0%
!! ACCTGGCTTTGCGGTGAAGG+TGG + chr1_1:7235348-7235367 None:intergenic 60.0%
GGATTCCACCTTGTCCCTCC+TGG + chr1_1:7235804-7235823 None:intergenic 60.0%
!! AGTGTGGTGGTGGGAGTTCG+AGG + chr1_1:7236029-7236048 None:intergenic 60.0%
GGGGGACACAGACACAAGTG+TGG + chr1_1:7236045-7236064 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 7235076 7236920 7235076 ID=Msa0004780;Name=Msa0004780
chr1_1 mRNA 7235076 7236920 7235076 ID=Msa0004780-mRNA-1;Parent=Msa0004780;Name=Msa0004780-mRNA-1;_AED=0.06;_eAED=0.06;_QI=50|1|1|1|1|1|3|411|236
chr1_1 exon 7235076 7235639 7235076 ID=Msa0004780-mRNA-1:exon:2526;Parent=Msa0004780-mRNA-1
chr1_1 exon 7235893 7236210 7235893 ID=Msa0004780-mRNA-1:exon:2525;Parent=Msa0004780-mRNA-1
chr1_1 exon 7236631 7236920 7236631 ID=Msa0004780-mRNA-1:exon:2524;Parent=Msa0004780-mRNA-1
chr1_1 five_prime_UTR 7236871 7236920 7236871 ID=Msa0004780-mRNA-1:five_prime_utr;Parent=Msa0004780-mRNA-1
chr1_1 CDS 7236631 7236870 7236631 ID=Msa0004780-mRNA-1:cds;Parent=Msa0004780-mRNA-1
chr1_1 CDS 7235893 7236210 7235893 ID=Msa0004780-mRNA-1:cds;Parent=Msa0004780-mRNA-1
chr1_1 CDS 7235487 7235639 7235487 ID=Msa0004780-mRNA-1:cds;Parent=Msa0004780-mRNA-1
chr1_1 three_prime_UTR 7235076 7235486 7235076 ID=Msa0004780-mRNA-1:three_prime_utr;Parent=Msa0004780-mRNA-1
Gene Sequence

>Msa0004780

ATGAAAGTGCATCAATTCACACGTGGATTTTGGGAGCATGAACCTTCCCTCACACTTGGTTGCAAGCGTTTACGTCCTCTTGCACCTAAAATTTCTTCTAATAACACCGATAATGTTACTTCTAACACCCCTTCTCTTTCTTTTGATCTCAAGAGCTTCATTAGACCCGAAAGTGCTCCTAGAATTAGTCTTTCTTCCTCTGATGATAACAAGAAAGATCCACCTTCACCGCAAAGCCAGGTTGAAACGCATATTCCAGGAGGGACAAGGTGGAATCCAACACAAGAACAAATAGGAATATTGGAGATGTTATACAGAGGTGGAATGAGAACTCCAAATGCACAACAAATAGAACAAATCACAGTTCAACTTAGCAAGTACGGTAAAATTGAAGGGAAAAATGTGTTCTATTGGTTCCAAAACCACAAAGCACGCGAGAGGCAAAAGCAGAAACGTAACAGTCTTGGCCTTCCTCATAGTCCTCGAACTCCCACCACCACACTTGTGTCTGTGTCCCCCACCTTTAGTACTATTACAACTTTGGACACCCCAAAAAGGGTGCAAGTAATGGAAAGAGATCAAGAAGATAGTCCATTGAAGAAGAGTAGGAGTTGGCCATTTGAGTACTTGGAAGAGAAAAATTGGTCAATGTGCAAAGTAGAGGAACATAAAACTCTAGAGCTTTTCCCATTACACCCAGAAGGCAGATGA
Protein sequence

>Msa0004780

MKVHQFTRGFWEHEPSLTLGCKRLRPLAPKISSNNTDNVTSNTPSLSFDLKSFIRPESAPRISLSSSDDNKKDPPSPQSQVETHIPGGTRWNPTQEQIGILEMLYRGGMRTPNAQQIEQITVQLSKYGKIEGKNVFYWFQNHKARERQKQKRNSLGLPHSPRTPTTTLVSVSPTFSTITTLDTPKRVQVMERDQEDSPLKKSRSWPFEYLEEKNWSMCKVEEHKTLELFPLHPEGR*