AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0144990

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0144990 MtrunA17_Chr1g0153171 98.305 236 4 0 1 236 1 236 1.06e-175 482
Msa0144990 MtrunA17_Chr8g0392991 45.255 137 69 1 22 152 22 158 2.24e-29 112
Msa0144990 MtrunA17_Chr7g0238431 71.642 67 19 0 86 152 4 70 5.78e-29 108
Msa0144990 MtrunA17_Chr4g0031341 36.932 176 81 4 90 235 25 200 1.81e-26 103
Msa0144990 MtrunA17_Chr5g0436661 65.152 66 23 0 90 155 33 98 8.85e-26 99.4
Msa0144990 MtrunA17_Chr4g0043891 68.750 64 20 0 89 152 7 70 1.13e-25 100
Msa0144990 MtrunA17_Chr5g0405351 64.062 64 22 1 89 151 29 92 1.20e-22 94.0
Msa0144990 MtrunA17_Chr3g0125111 40.146 137 60 2 22 152 22 142 6.47e-22 88.6
Msa0144990 MtrunA17_Chr8g0385441 76.923 52 12 0 101 152 93 144 2.76e-21 87.0
Msa0144990 MtrunA17_Chr2g0283141 35.577 104 55 1 49 152 26 117 3.87e-14 71.2
Msa0144990 MtrunA17_Chr7g0223471 46.774 62 33 0 90 151 47 108 1.68e-13 68.9
Msa0144990 MtrunA17_Chr3g0144021 39.474 76 46 0 90 165 82 157 3.20e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0144990 AT1G46480.1 52.896 259 91 9 1 236 1 251 1.63e-72 221
Msa0144990 AT1G46480.2 59.067 193 60 7 1 182 1 185 3.05e-63 196
Msa0144990 AT3G18010.1 42.857 147 74 2 6 152 2 138 6.13e-30 114
Msa0144990 AT3G11260.1 56.818 88 33 1 87 169 16 103 1.24e-27 104
Msa0144990 AT5G59340.1 61.333 75 29 0 78 152 2 76 5.75e-27 104
Msa0144990 AT2G28610.1 68.182 66 21 0 87 152 5 70 9.84e-27 103
Msa0144990 AT5G05770.1 63.077 65 24 0 90 154 29 93 1.72e-24 94.4
Msa0144990 AT2G17950.1 51.765 85 40 1 89 172 37 121 1.05e-23 96.7
Msa0144990 AT2G01500.1 66.667 63 19 1 84 144 53 115 2.07e-21 90.1
Msa0144990 AT2G33880.2 38.614 101 52 1 49 149 24 114 4.42e-14 71.2
Msa0144990 AT2G33880.1 38.614 101 52 1 49 149 24 114 4.70e-14 70.9
Msa0144990 AT1G20700.2 47.541 61 32 0 90 150 33 93 2.70e-13 65.9
Msa0144990 AT5G45980.1 46.774 62 33 0 90 151 55 116 4.97e-13 67.8
Msa0144990 AT1G20710.1 38.462 91 44 1 97 187 86 164 6.50e-13 65.9
Msa0144990 AT1G20700.3 47.541 61 32 0 90 150 95 155 9.15e-13 65.5
Msa0144990 AT1G20700.1 47.541 61 32 0 90 150 95 155 1.09e-12 65.5
Msa0144990 AT4G35550.1 47.541 61 32 0 90 150 99 159 1.15e-11 63.2

Find 71 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAATTCACACGTGGATTT+TGG 0.121803 1_4:-4571406 Msa0144990:CDS
ACAAATAAGTTTGGTTAATT+TGG 0.133382 1_4:+4569854 None:intergenic
AAGCAAATTAAAGCACATTT+TGG 0.180958 1_4:+4569986 None:intergenic
TCTCGCGTGCTTTGTGGTTT+TGG 0.203041 1_4:+4570572 None:intergenic
AAACTCCTTCATCTGCCTTC+TGG 0.237007 1_4:+4570038 None:intergenic
AACTCCTTCATCTGCCTTCT+GGG 0.285945 1_4:+4570039 None:intergenic
CAACTTTGGACACCCCAAAA+AGG 0.296385 1_4:-4570453 Msa0144990:CDS
ACTAATTCTAGGAGCACTTT+CGG 0.300053 1_4:+4571250 None:intergenic
TGTTCTATTTGTTGTGCATT+TGG 0.303503 1_4:+4570655 None:intergenic
CTAATTCTAGGAGCACTTTC+GGG 0.314910 1_4:+4571251 None:intergenic
ACAAGAACAAATAGGAATAT+TGG 0.328145 1_4:-4570707 Msa0144990:CDS
GAGGAAGAAAGACTAATTCT+AGG 0.342909 1_4:+4571239 None:intergenic
GGATTCCACCTTGTCCCTCC+TGG 0.366142 1_4:+4570733 None:intergenic
ATCTGCCTTCTGGGTGTAAT+GGG 0.376276 1_4:+4570048 None:intergenic
CTTTAGTACTATTACAACTT+TGG 0.384910 1_4:-4570467 Msa0144990:CDS
CATCTGCCTTCTGGGTGTAA+TGG 0.398760 1_4:+4570047 None:intergenic
ATTCCTATTTGTTCTTGTGT+TGG 0.415008 1_4:+4570712 None:intergenic
TTTCTTCTTTCATTTACATT+GGG 0.415761 1_4:-4569953 Msa0144990:three_prime_UTR
AATCCAACACAAGAACAAAT+AGG 0.426122 1_4:-4570715 Msa0144990:CDS
GTAATGTTATTCCATGACTT+TGG 0.426731 1_4:-4569879 Msa0144990:three_prime_UTR
TTCTCTTCCAAGTACTCAAA+TGG 0.427494 1_4:+4570119 None:intergenic
ATTTCTTCTTTCATTTACAT+TGG 0.443688 1_4:-4569954 Msa0144990:three_prime_UTR
TTTGTCTCTCGCGTGCTTTG+TGG 0.448508 1_4:+4570566 None:intergenic
TAGGTTGAAACGCATATTCC+AGG 0.450427 1_4:-4570751 Msa0144990:CDS
TCCACCTTCACCGCAAAGCC+AGG 0.461999 1_4:-4571198 Msa0144990:intron
TTTGTTGATTTCAGCGTAGT+AGG 0.464270 1_4:-4569817 Msa0144990:three_prime_UTR
GAGTTGGCCATTTGAGTACT+TGG 0.464745 1_4:-4570126 Msa0144990:CDS
GGTGTTAGAAGTAACATTAT+CGG 0.465029 1_4:+4571310 None:intergenic
CAGCGTAGTAGGTGTGACTT+TGG 0.468785 1_4:-4569806 Msa0144990:three_prime_UTR
CACAGACACAAGTGTGGTGG+TGG 0.469900 1_4:+4570498 None:intergenic
CAAAGTTGTAATAGTACTAA+AGG 0.472738 1_4:+4570468 None:intergenic
AAGATAAGATTAGTAGAAGT+AGG 0.473815 1_4:+4569691 None:intergenic
AGCAAGTACGGTAAAATTGA+AGG 0.477134 1_4:-4570616 Msa0144990:CDS
CAACTCCTACTCTTCTTCAA+TGG 0.501838 1_4:+4570143 None:intergenic
AGTGTGGTGGTGGGAGTTCG+AGG 0.518160 1_4:+4570508 None:intergenic
CATGAACCTTCCCTCACACT+TGG 0.520457 1_4:-4571380 Msa0144990:CDS
TGAACCTGGCTTTGCGGTGA+AGG 0.521265 1_4:+4571194 None:intergenic
GTTGTAATAGTACTAAAGGT+GGG 0.528602 1_4:+4570472 None:intergenic
TGAGATCAAAAGAAAGAGAA+GGG 0.531578 1_4:+4571288 None:intergenic
TTAATTTGGCTCCAAAGTCA+TGG 0.533407 1_4:+4569868 None:intergenic
AGTATATGAACCTGGCTTTG+CGG 0.544738 1_4:+4571188 None:intergenic
ATAGTCCATTGAAGAAGAGT+AGG 0.545832 1_4:-4570148 Msa0144990:CDS
AAGCAGAAACGTAACAGTCT+TGG 0.547628 1_4:-4570544 Msa0144990:CDS
ATCTTTCTTGTTATCATCAG+AGG 0.556963 1_4:+4571220 None:intergenic
ACAGTTCAACTTAGCAAGTA+CGG 0.562866 1_4:-4570628 Msa0144990:CDS
CGCATATTCCAGGAGGGACA+AGG 0.565252 1_4:-4570741 Msa0144990:CDS
TACACCCAGAAGGCAGATGA+AGG 0.566613 1_4:-4570043 Msa0144990:exon
GTGGGAGTTCGAGGACTATG+AGG 0.567688 1_4:+4570517 None:intergenic
TTGGTCAATGTGCAAAGTAG+AGG 0.568050 1_4:-4570093 Msa0144990:CDS
TTGAGATCAAAAGAAAGAGA+AGG 0.574637 1_4:+4571287 None:intergenic
AGTTGTAATAGTACTAAAGG+TGG 0.581733 1_4:+4570471 None:intergenic
AACGCTTGCAACCAAGTGTG+AGG 0.582297 1_4:+4571369 None:intergenic
GCAAGTACGGTAAAATTGAA+GGG 0.588273 1_4:-4570615 Msa0144990:CDS
CATTGAAGAAGAGTAGGAGT+TGG 0.590517 1_4:-4570142 Msa0144990:CDS
ACCTGGCTTTGCGGTGAAGG+TGG 0.590968 1_4:+4571197 None:intergenic
TGTAATAGTACTAAAGGTGG+GGG 0.603492 1_4:+4570474 None:intergenic
TTGTAATAGTACTAAAGGTG+GGG 0.606911 1_4:+4570473 None:intergenic
TTGGAGATGTTATACAGAGG+TGG 0.611718 1_4:-4570688 Msa0144990:CDS
GAGTTCGAGGACTATGAGGA+AGG 0.633348 1_4:+4570521 None:intergenic
GATTAGTAGAAGTAGGTGCG+TGG 0.641260 1_4:+4569698 None:intergenic
TATTTCTTCATCAGACCGTG+TGG 0.650611 1_4:-4571466 Msa0144990:five_prime_UTR
ACGCTTGCAACCAAGTGTGA+GGG 0.656705 1_4:+4571370 None:intergenic
TTGAAACGCATATTCCAGGA+GGG 0.657126 1_4:-4570747 Msa0144990:CDS
ACAGACACAAGTGTGGTGGT+GGG 0.659851 1_4:+4570499 None:intergenic
GGACACAGACACAAGTGTGG+TGG 0.666759 1_4:+4570495 None:intergenic
GAGATCAAAAGAAAGAGAAG+GGG 0.671823 1_4:+4571289 None:intergenic
TTGCAACCAAGTGTGAGGGA+AGG 0.674259 1_4:+4571374 None:intergenic
GTTGAAACGCATATTCCAGG+AGG 0.708848 1_4:-4570748 Msa0144990:CDS
ATATTGGAGATGTTATACAG+AGG 0.712720 1_4:-4570691 Msa0144990:CDS
AAAGTGCATCAATTCACACG+TGG 0.742454 1_4:-4571413 Msa0144990:CDS
ATATTCCAGGAGGGACAAGG+TGG 0.756182 1_4:-4570738 Msa0144990:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATACTAATAAAGTATTT+TGG - chr1_4:4570116-4570135 Msa0144990:CDS 10.0%
!!! ATAATACTAATAAAGTATTT+TGG - chr1_4:4570116-4570135 Msa0144990:CDS 10.0%
!! CAGACTTAATTAAAAAAAAA+AGG + chr1_4:4569960-4569979 None:intergenic 15.0%
!! AAATCAACAAATGAAAAAAA+AGG + chr1_4:4571275-4571294 None:intergenic 15.0%
!! CAGACTTAATTAAAAAAAAA+AGG + chr1_4:4569960-4569979 None:intergenic 15.0%
!! AAATCAACAAATGAAAAAAA+AGG + chr1_4:4571275-4571294 None:intergenic 15.0%
!!! GTTTAATATAATTTTCCACA+CGG + chr1_4:4569653-4569672 None:intergenic 20.0%
!!! GTGTTATTAGAAGAAATTTT+AGG + chr1_4:4569772-4569791 None:intergenic 20.0%
!! ATTAACTTCATGTAACATAT+AGG - chr1_4:4570073-4570092 Msa0144990:CDS 20.0%
!! TTAACTTCATGTAACATATA+GGG - chr1_4:4570074-4570093 Msa0144990:CDS 20.0%
!! TCTATTATTTGTGTATGTAT+AGG - chr1_4:4570331-4570350 Msa0144990:intron 20.0%
!!! TTTTTGATTAAAAGGAAAGT+AGG - chr1_4:4570795-4570814 Msa0144990:intron 20.0%
!!! TTGTTCATGGTTTTAAATTA+CGG + chr1_4:4570822-4570841 None:intergenic 20.0%
!! AATTAGTATACTATTGTTCA+TGG + chr1_4:4570835-4570854 None:intergenic 20.0%
!! AATACTATAATCTTGTTACT+AGG - chr1_4:4570857-4570876 Msa0144990:intron 20.0%
!! ATTTCTTCTTTCATTTACAT+TGG - chr1_4:4571147-4571166 Msa0144990:intron 20.0%
!! TTTCTTCTTTCATTTACATT+GGG - chr1_4:4571148-4571167 Msa0144990:intron 20.0%
!! ACAAATAAGTTTGGTTAATT+TGG + chr1_4:4571250-4571269 None:intergenic 20.0%
!!! ATACTATACTCTCATATTTT+AGG - chr1_4:4571448-4571467 Msa0144990:five_prime_UTR 20.0%
!!! GTTTAATATAATTTTCCACA+CGG + chr1_4:4569653-4569672 None:intergenic 20.0%
!!! GTGTTATTAGAAGAAATTTT+AGG + chr1_4:4569772-4569791 None:intergenic 20.0%
!! ATTAACTTCATGTAACATAT+AGG - chr1_4:4570073-4570092 Msa0144990:CDS 20.0%
!! TTAACTTCATGTAACATATA+GGG - chr1_4:4570074-4570093 Msa0144990:CDS 20.0%
!! TCTATTATTTGTGTATGTAT+AGG - chr1_4:4570331-4570350 Msa0144990:intron 20.0%
!!! TTTTTGATTAAAAGGAAAGT+AGG - chr1_4:4570795-4570814 Msa0144990:intron 20.0%
!!! TTGTTCATGGTTTTAAATTA+CGG + chr1_4:4570822-4570841 None:intergenic 20.0%
!! AATTAGTATACTATTGTTCA+TGG + chr1_4:4570835-4570854 None:intergenic 20.0%
!! AATACTATAATCTTGTTACT+AGG - chr1_4:4570857-4570876 Msa0144990:intron 20.0%
!! ATTTCTTCTTTCATTTACAT+TGG - chr1_4:4571147-4571166 Msa0144990:intron 20.0%
!! TTTCTTCTTTCATTTACATT+GGG - chr1_4:4571148-4571167 Msa0144990:intron 20.0%
!! ACAAATAAGTTTGGTTAATT+TGG + chr1_4:4571250-4571269 None:intergenic 20.0%
!!! ATACTATACTCTCATATTTT+AGG - chr1_4:4571448-4571467 Msa0144990:five_prime_UTR 20.0%
!!! ACTAATAAAGTATTTTGGTC+CGG - chr1_4:4570121-4570140 Msa0144990:CDS 25.0%
! TACATCTGATAACTGAAATT+CGG + chr1_4:4570161-4570180 None:intergenic 25.0%
!!! TCGTTTAAAAGCTTTTGATT+TGG - chr1_4:4570281-4570300 Msa0144990:intron 25.0%
! ACAAGAACAAATAGGAATAT+TGG - chr1_4:4570394-4570413 Msa0144990:intron 25.0%
! CAAAGTTGTAATAGTACTAA+AGG + chr1_4:4570636-4570655 None:intergenic 25.0%
! CTTTAGTACTATTACAACTT+TGG - chr1_4:4570634-4570653 Msa0144990:CDS 25.0%
!! AATATGAACATACCCTTTTT+GGG + chr1_4:4570664-4570683 None:intergenic 25.0%
! TCAACTTAAGCTATATATTG+TGG - chr1_4:4570694-4570713 Msa0144990:CDS 25.0%
!! CGACAATATAAAGGATTTTA+AGG + chr1_4:4570742-4570761 None:intergenic 25.0%
!!! TCGAATGTGTTACATTTTTT+TGG - chr1_4:4570898-4570917 Msa0144990:intron 25.0%
!! AAGCAAATTAAAGCACATTT+TGG + chr1_4:4571118-4571137 None:intergenic 25.0%
! AAAAAGGTGACAAATAAGTT+TGG + chr1_4:4571259-4571278 None:intergenic 25.0%
! AAGATAAGATTAGTAGAAGT+AGG + chr1_4:4571413-4571432 None:intergenic 25.0%
!!! ACTAATAAAGTATTTTGGTC+CGG - chr1_4:4570121-4570140 Msa0144990:CDS 25.0%
! TACATCTGATAACTGAAATT+CGG + chr1_4:4570161-4570180 None:intergenic 25.0%
!!! TCGTTTAAAAGCTTTTGATT+TGG - chr1_4:4570281-4570300 Msa0144990:intron 25.0%
! ACAAGAACAAATAGGAATAT+TGG - chr1_4:4570394-4570413 Msa0144990:intron 25.0%
! CAAAGTTGTAATAGTACTAA+AGG + chr1_4:4570636-4570655 None:intergenic 25.0%
! CTTTAGTACTATTACAACTT+TGG - chr1_4:4570634-4570653 Msa0144990:CDS 25.0%
!! AATATGAACATACCCTTTTT+GGG + chr1_4:4570664-4570683 None:intergenic 25.0%
! TCAACTTAAGCTATATATTG+TGG - chr1_4:4570694-4570713 Msa0144990:CDS 25.0%
!! CGACAATATAAAGGATTTTA+AGG + chr1_4:4570742-4570761 None:intergenic 25.0%
!!! TCGAATGTGTTACATTTTTT+TGG - chr1_4:4570898-4570917 Msa0144990:intron 25.0%
!! AAGCAAATTAAAGCACATTT+TGG + chr1_4:4571118-4571137 None:intergenic 25.0%
! AAAAAGGTGACAAATAAGTT+TGG + chr1_4:4571259-4571278 None:intergenic 25.0%
! AAGATAAGATTAGTAGAAGT+AGG + chr1_4:4571413-4571432 None:intergenic 25.0%
GGTGTTAGAAGTAACATTAT+CGG + chr1_4:4569794-4569813 None:intergenic 30.0%
TGAGATCAAAAGAAAGAGAA+GGG + chr1_4:4569816-4569835 None:intergenic 30.0%
TTGAGATCAAAAGAAAGAGA+AGG + chr1_4:4569817-4569836 None:intergenic 30.0%
! ATCTTTCTTGTTATCATCAG+AGG + chr1_4:4569884-4569903 None:intergenic 30.0%
!!! CTAATAAAGTATTTTGGTCC+GGG - chr1_4:4570122-4570141 Msa0144990:CDS 30.0%
AATCCAACACAAGAACAAAT+AGG - chr1_4:4570386-4570405 Msa0144990:intron 30.0%
ATTCCTATTTGTTCTTGTGT+TGG + chr1_4:4570392-4570411 None:intergenic 30.0%
ATATTGGAGATGTTATACAG+AGG - chr1_4:4570410-4570429 Msa0144990:intron 30.0%
!! TGTTCTATTTGTTGTGCATT+TGG + chr1_4:4570449-4570468 None:intergenic 30.0%
AAGGGAAAAATGTGTTCTAT+TGG - chr1_4:4570504-4570523 Msa0144990:CDS 30.0%
TTGTAATAGTACTAAAGGTG+GGG + chr1_4:4570631-4570650 None:intergenic 30.0%
GTTGTAATAGTACTAAAGGT+GGG + chr1_4:4570632-4570651 None:intergenic 30.0%
AGTTGTAATAGTACTAAAGG+TGG + chr1_4:4570633-4570652 None:intergenic 30.0%
! ATATGAACATACCCTTTTTG+GGG + chr1_4:4570663-4570682 None:intergenic 30.0%
! GAATATGAACATACCCTTTT+TGG + chr1_4:4570665-4570684 None:intergenic 30.0%
!!! TGTGTTACATTTTTTTGGAC+AGG - chr1_4:4570903-4570922 Msa0144990:intron 30.0%
AGTACTTGGAAGAGAAAAAT+TGG - chr1_4:4570989-4571008 Msa0144990:intron 30.0%
GTAATGTTATTCCATGACTT+TGG - chr1_4:4571222-4571241 Msa0144990:CDS 30.0%
AAGCTTATGTCGTCTAAAAA+AGG + chr1_4:4571332-4571351 None:intergenic 30.0%
!!! TCGAAAGCTTTTAGGTTAAA+AGG - chr1_4:4571357-4571376 Msa0144990:CDS 30.0%
GGTGTTAGAAGTAACATTAT+CGG + chr1_4:4569794-4569813 None:intergenic 30.0%
TGAGATCAAAAGAAAGAGAA+GGG + chr1_4:4569816-4569835 None:intergenic 30.0%
TTGAGATCAAAAGAAAGAGA+AGG + chr1_4:4569817-4569836 None:intergenic 30.0%
! ATCTTTCTTGTTATCATCAG+AGG + chr1_4:4569884-4569903 None:intergenic 30.0%
!!! CTAATAAAGTATTTTGGTCC+GGG - chr1_4:4570122-4570141 Msa0144990:CDS 30.0%
AATCCAACACAAGAACAAAT+AGG - chr1_4:4570386-4570405 Msa0144990:intron 30.0%
ATTCCTATTTGTTCTTGTGT+TGG + chr1_4:4570392-4570411 None:intergenic 30.0%
ATATTGGAGATGTTATACAG+AGG - chr1_4:4570410-4570429 Msa0144990:intron 30.0%
!! TGTTCTATTTGTTGTGCATT+TGG + chr1_4:4570449-4570468 None:intergenic 30.0%
AAGGGAAAAATGTGTTCTAT+TGG - chr1_4:4570504-4570523 Msa0144990:CDS 30.0%
TTGTAATAGTACTAAAGGTG+GGG + chr1_4:4570631-4570650 None:intergenic 30.0%
GTTGTAATAGTACTAAAGGT+GGG + chr1_4:4570632-4570651 None:intergenic 30.0%
AGTTGTAATAGTACTAAAGG+TGG + chr1_4:4570633-4570652 None:intergenic 30.0%
! ATATGAACATACCCTTTTTG+GGG + chr1_4:4570663-4570682 None:intergenic 30.0%
! GAATATGAACATACCCTTTT+TGG + chr1_4:4570665-4570684 None:intergenic 30.0%
!!! TGTGTTACATTTTTTTGGAC+AGG - chr1_4:4570903-4570922 Msa0144990:intron 30.0%
AGTACTTGGAAGAGAAAAAT+TGG - chr1_4:4570989-4571008 Msa0144990:intron 30.0%
GTAATGTTATTCCATGACTT+TGG - chr1_4:4571222-4571241 Msa0144990:CDS 30.0%
AAGCTTATGTCGTCTAAAAA+AGG + chr1_4:4571332-4571351 None:intergenic 30.0%
!!! TCGAAAGCTTTTAGGTTAAA+AGG - chr1_4:4571357-4571376 Msa0144990:CDS 30.0%
! ATCAATTCACACGTGGATTT+TGG - chr1_4:4569695-4569714 Msa0144990:three_prime_UTR 35.0%
! TCAATTCACACGTGGATTTT+GGG - chr1_4:4569696-4569715 Msa0144990:three_prime_UTR 35.0%
!! GAAGAAATTTTAGGTGCAAG+AGG + chr1_4:4569763-4569782 None:intergenic 35.0%
GAGATCAAAAGAAAGAGAAG+GGG + chr1_4:4569815-4569834 None:intergenic 35.0%
!! ACTAATTCTAGGAGCACTTT+CGG + chr1_4:4569854-4569873 None:intergenic 35.0%
GAGGAAGAAAGACTAATTCT+AGG + chr1_4:4569865-4569884 None:intergenic 35.0%
TAGAGCTTAGTATATGAACC+TGG + chr1_4:4569924-4569943 None:intergenic 35.0%
AATTATACCACTCTACTGCA+TGG + chr1_4:4569988-4570007 None:intergenic 35.0%
! TTCGGTTCAACAATTTTTCC+CGG + chr1_4:4570143-4570162 None:intergenic 35.0%
ACAGTTCAACTTAGCAAGTA+CGG - chr1_4:4570473-4570492 Msa0144990:CDS 35.0%
AGCAAGTACGGTAAAATTGA+AGG - chr1_4:4570485-4570504 Msa0144990:CDS 35.0%
GCAAGTACGGTAAAATTGAA+GGG - chr1_4:4570486-4570505 Msa0144990:CDS 35.0%
TGTAATAGTACTAAAGGTGG+GGG + chr1_4:4570630-4570649 None:intergenic 35.0%
! TAGTTGCGTCGACAATATAA+AGG + chr1_4:4570751-4570770 None:intergenic 35.0%
ATAGTCCATTGAAGAAGAGT+AGG - chr1_4:4570953-4570972 Msa0144990:intron 35.0%
TTCTCTTCCAAGTACTCAAA+TGG + chr1_4:4570985-4571004 None:intergenic 35.0%
! TTAATTTGGCTCCAAAGTCA+TGG + chr1_4:4571236-4571255 None:intergenic 35.0%
!! TTTGTTGATTTCAGCGTAGT+AGG - chr1_4:4571284-4571303 Msa0144990:CDS 35.0%
! CTTGTGAATCGAAAGCTTTT+AGG - chr1_4:4571349-4571368 Msa0144990:CDS 35.0%
! ATCAATTCACACGTGGATTT+TGG - chr1_4:4569695-4569714 Msa0144990:three_prime_UTR 35.0%
! TCAATTCACACGTGGATTTT+GGG - chr1_4:4569696-4569715 Msa0144990:three_prime_UTR 35.0%
!! GAAGAAATTTTAGGTGCAAG+AGG + chr1_4:4569763-4569782 None:intergenic 35.0%
GAGATCAAAAGAAAGAGAAG+GGG + chr1_4:4569815-4569834 None:intergenic 35.0%
!! ACTAATTCTAGGAGCACTTT+CGG + chr1_4:4569854-4569873 None:intergenic 35.0%
GAGGAAGAAAGACTAATTCT+AGG + chr1_4:4569865-4569884 None:intergenic 35.0%
TAGAGCTTAGTATATGAACC+TGG + chr1_4:4569924-4569943 None:intergenic 35.0%
AATTATACCACTCTACTGCA+TGG + chr1_4:4569988-4570007 None:intergenic 35.0%
! TTCGGTTCAACAATTTTTCC+CGG + chr1_4:4570143-4570162 None:intergenic 35.0%
ACAGTTCAACTTAGCAAGTA+CGG - chr1_4:4570473-4570492 Msa0144990:CDS 35.0%
AGCAAGTACGGTAAAATTGA+AGG - chr1_4:4570485-4570504 Msa0144990:CDS 35.0%
GCAAGTACGGTAAAATTGAA+GGG - chr1_4:4570486-4570505 Msa0144990:CDS 35.0%
TGTAATAGTACTAAAGGTGG+GGG + chr1_4:4570630-4570649 None:intergenic 35.0%
! TAGTTGCGTCGACAATATAA+AGG + chr1_4:4570751-4570770 None:intergenic 35.0%
ATAGTCCATTGAAGAAGAGT+AGG - chr1_4:4570953-4570972 Msa0144990:intron 35.0%
TTCTCTTCCAAGTACTCAAA+TGG + chr1_4:4570985-4571004 None:intergenic 35.0%
! TTAATTTGGCTCCAAAGTCA+TGG + chr1_4:4571236-4571255 None:intergenic 35.0%
!! TTTGTTGATTTCAGCGTAGT+AGG - chr1_4:4571284-4571303 Msa0144990:CDS 35.0%
! CTTGTGAATCGAAAGCTTTT+AGG - chr1_4:4571349-4571368 Msa0144990:CDS 35.0%
TATTTCTTCATCAGACCGTG+TGG - chr1_4:4569635-4569654 Msa0144990:three_prime_UTR 40.0%
AAAGTGCATCAATTCACACG+TGG - chr1_4:4569688-4569707 Msa0144990:three_prime_UTR 40.0%
!! CTAATTCTAGGAGCACTTTC+GGG + chr1_4:4569853-4569872 None:intergenic 40.0%
!! AGTATATGAACCTGGCTTTG+CGG + chr1_4:4569916-4569935 None:intergenic 40.0%
CGAGTCTCATATGGAAAAAG+AGG + chr1_4:4570223-4570242 None:intergenic 40.0%
AGTAACTCTCGAGTCTCATA+TGG + chr1_4:4570232-4570251 None:intergenic 40.0%
! TAGGTTGAAACGCATATTCC+AGG - chr1_4:4570350-4570369 Msa0144990:intron 40.0%
TTGAAACGCATATTCCAGGA+GGG - chr1_4:4570354-4570373 Msa0144990:intron 40.0%
TTGGAGATGTTATACAGAGG+TGG - chr1_4:4570413-4570432 Msa0144990:intron 40.0%
AAGCAGAAACGTAACAGTCT+TGG - chr1_4:4570557-4570576 Msa0144990:CDS 40.0%
AACTTTGGACACCCCAAAAA+GGG - chr1_4:4570649-4570668 Msa0144990:CDS 40.0%
! TTTTGGACAGGTGCAAGTAA+TGG - chr1_4:4570915-4570934 Msa0144990:intron 40.0%
CAACTCCTACTCTTCTTCAA+TGG + chr1_4:4570961-4570980 None:intergenic 40.0%
CATTGAAGAAGAGTAGGAGT+TGG - chr1_4:4570959-4570978 Msa0144990:intron 40.0%
TTGGTCAATGTGCAAAGTAG+AGG - chr1_4:4571008-4571027 Msa0144990:intron 40.0%
TATTTCTTCATCAGACCGTG+TGG - chr1_4:4569635-4569654 Msa0144990:three_prime_UTR 40.0%
AAAGTGCATCAATTCACACG+TGG - chr1_4:4569688-4569707 Msa0144990:three_prime_UTR 40.0%
!! CTAATTCTAGGAGCACTTTC+GGG + chr1_4:4569853-4569872 None:intergenic 40.0%
!! AGTATATGAACCTGGCTTTG+CGG + chr1_4:4569916-4569935 None:intergenic 40.0%
CGAGTCTCATATGGAAAAAG+AGG + chr1_4:4570223-4570242 None:intergenic 40.0%
AGTAACTCTCGAGTCTCATA+TGG + chr1_4:4570232-4570251 None:intergenic 40.0%
! TAGGTTGAAACGCATATTCC+AGG - chr1_4:4570350-4570369 Msa0144990:intron 40.0%
TTGAAACGCATATTCCAGGA+GGG - chr1_4:4570354-4570373 Msa0144990:intron 40.0%
TTGGAGATGTTATACAGAGG+TGG - chr1_4:4570413-4570432 Msa0144990:intron 40.0%
AAGCAGAAACGTAACAGTCT+TGG - chr1_4:4570557-4570576 Msa0144990:CDS 40.0%
AACTTTGGACACCCCAAAAA+GGG - chr1_4:4570649-4570668 Msa0144990:CDS 40.0%
! TTTTGGACAGGTGCAAGTAA+TGG - chr1_4:4570915-4570934 Msa0144990:intron 40.0%
CAACTCCTACTCTTCTTCAA+TGG + chr1_4:4570961-4570980 None:intergenic 40.0%
CATTGAAGAAGAGTAGGAGT+TGG - chr1_4:4570959-4570978 Msa0144990:intron 40.0%
TTGGTCAATGTGCAAAGTAG+AGG - chr1_4:4571008-4571027 Msa0144990:intron 40.0%
ATACCACTCTACTGCATGGA+TGG + chr1_4:4569984-4570003 None:intergenic 45.0%
GTTGAAACGCATATTCCAGG+AGG - chr1_4:4570353-4570372 Msa0144990:intron 45.0%
CAACTTTGGACACCCCAAAA+AGG - chr1_4:4570648-4570667 Msa0144990:CDS 45.0%
TGTCGACGCAACTATAGTCT+TGG - chr1_4:4570757-4570776 Msa0144990:intron 45.0%
GAGTTGGCCATTTGAGTACT+TGG - chr1_4:4570975-4570994 Msa0144990:intron 45.0%
! CTTTTCCCATTACACCCAGA+AGG - chr1_4:4571048-4571067 Msa0144990:intron 45.0%
ATCTGCCTTCTGGGTGTAAT+GGG + chr1_4:4571056-4571075 None:intergenic 45.0%
AACTCCTTCATCTGCCTTCT+GGG + chr1_4:4571065-4571084 None:intergenic 45.0%
AAACTCCTTCATCTGCCTTC+TGG + chr1_4:4571066-4571085 None:intergenic 45.0%
GATTAGTAGAAGTAGGTGCG+TGG + chr1_4:4571406-4571425 None:intergenic 45.0%
ATACCACTCTACTGCATGGA+TGG + chr1_4:4569984-4570003 None:intergenic 45.0%
GTTGAAACGCATATTCCAGG+AGG - chr1_4:4570353-4570372 Msa0144990:intron 45.0%
CAACTTTGGACACCCCAAAA+AGG - chr1_4:4570648-4570667 Msa0144990:CDS 45.0%
TGTCGACGCAACTATAGTCT+TGG - chr1_4:4570757-4570776 Msa0144990:intron 45.0%
GAGTTGGCCATTTGAGTACT+TGG - chr1_4:4570975-4570994 Msa0144990:intron 45.0%
! CTTTTCCCATTACACCCAGA+AGG - chr1_4:4571048-4571067 Msa0144990:intron 45.0%
ATCTGCCTTCTGGGTGTAAT+GGG + chr1_4:4571056-4571075 None:intergenic 45.0%
AACTCCTTCATCTGCCTTCT+GGG + chr1_4:4571065-4571084 None:intergenic 45.0%
AAACTCCTTCATCTGCCTTC+TGG + chr1_4:4571066-4571085 None:intergenic 45.0%
GATTAGTAGAAGTAGGTGCG+TGG + chr1_4:4571406-4571425 None:intergenic 45.0%
!! AATCAAAAAAAAAAAAATTA+CGG + chr1_4:4570784-4570803 None:intergenic 5.0%
!!! TTTTTTTTTTTTTGATTAAA+AGG - chr1_4:4570787-4570806 Msa0144990:intron 5.0%
!! AATCAAAAAAAAAAAAATTA+CGG + chr1_4:4570784-4570803 None:intergenic 5.0%
!!! TTTTTTTTTTTTTGATTAAA+AGG - chr1_4:4570787-4570806 Msa0144990:intron 5.0%
CATGAACCTTCCCTCACACT+TGG - chr1_4:4569721-4569740 Msa0144990:three_prime_UTR 50.0%
TTGCAACCAAGTGTGAGGGA+AGG + chr1_4:4569730-4569749 None:intergenic 50.0%
ACGCTTGCAACCAAGTGTGA+GGG + chr1_4:4569734-4569753 None:intergenic 50.0%
AACGCTTGCAACCAAGTGTG+AGG + chr1_4:4569735-4569754 None:intergenic 50.0%
TGTCCATCCATGCAGTAGAG+TGG - chr1_4:4569978-4569997 Msa0144990:three_prime_UTR 50.0%
ATATTCCAGGAGGGACAAGG+TGG - chr1_4:4570363-4570382 Msa0144990:intron 50.0%
! TCTCGCGTGCTTTGTGGTTT+TGG + chr1_4:4570532-4570551 None:intergenic 50.0%
TTTGTCTCTCGCGTGCTTTG+TGG + chr1_4:4570538-4570557 None:intergenic 50.0%
GAGTTCGAGGACTATGAGGA+AGG + chr1_4:4570583-4570602 None:intergenic 50.0%
ACAGACACAAGTGTGGTGGT+GGG + chr1_4:4570605-4570624 None:intergenic 50.0%
AGGTCTCTGCAACGACGTTT+CGG + chr1_4:4570722-4570741 None:intergenic 50.0%
CATCTGCCTTCTGGGTGTAA+TGG + chr1_4:4571057-4571076 None:intergenic 50.0%
TACACCCAGAAGGCAGATGA+AGG - chr1_4:4571058-4571077 Msa0144990:intron 50.0%
CAGCGTAGTAGGTGTGACTT+TGG - chr1_4:4571295-4571314 Msa0144990:CDS 50.0%
CATGAACCTTCCCTCACACT+TGG - chr1_4:4569721-4569740 Msa0144990:three_prime_UTR 50.0%
TTGCAACCAAGTGTGAGGGA+AGG + chr1_4:4569730-4569749 None:intergenic 50.0%
ACGCTTGCAACCAAGTGTGA+GGG + chr1_4:4569734-4569753 None:intergenic 50.0%
AACGCTTGCAACCAAGTGTG+AGG + chr1_4:4569735-4569754 None:intergenic 50.0%
TGTCCATCCATGCAGTAGAG+TGG - chr1_4:4569978-4569997 Msa0144990:three_prime_UTR 50.0%
ATATTCCAGGAGGGACAAGG+TGG - chr1_4:4570363-4570382 Msa0144990:intron 50.0%
! TCTCGCGTGCTTTGTGGTTT+TGG + chr1_4:4570532-4570551 None:intergenic 50.0%
TTTGTCTCTCGCGTGCTTTG+TGG + chr1_4:4570538-4570557 None:intergenic 50.0%
GAGTTCGAGGACTATGAGGA+AGG + chr1_4:4570583-4570602 None:intergenic 50.0%
ACAGACACAAGTGTGGTGGT+GGG + chr1_4:4570605-4570624 None:intergenic 50.0%
AGGTCTCTGCAACGACGTTT+CGG + chr1_4:4570722-4570741 None:intergenic 50.0%
CATCTGCCTTCTGGGTGTAA+TGG + chr1_4:4571057-4571076 None:intergenic 50.0%
TACACCCAGAAGGCAGATGA+AGG - chr1_4:4571058-4571077 Msa0144990:intron 50.0%
CAGCGTAGTAGGTGTGACTT+TGG - chr1_4:4571295-4571314 Msa0144990:CDS 50.0%
!! TGAACCTGGCTTTGCGGTGA+AGG + chr1_4:4569910-4569929 None:intergenic 55.0%
CGCATATTCCAGGAGGGACA+AGG - chr1_4:4570360-4570379 Msa0144990:intron 55.0%
GTGGGAGTTCGAGGACTATG+AGG + chr1_4:4570587-4570606 None:intergenic 55.0%
CACAGACACAAGTGTGGTGG+TGG + chr1_4:4570606-4570625 None:intergenic 55.0%
GGACACAGACACAAGTGTGG+TGG + chr1_4:4570609-4570628 None:intergenic 55.0%
!! TGAACCTGGCTTTGCGGTGA+AGG + chr1_4:4569910-4569929 None:intergenic 55.0%
CGCATATTCCAGGAGGGACA+AGG - chr1_4:4570360-4570379 Msa0144990:intron 55.0%
GTGGGAGTTCGAGGACTATG+AGG + chr1_4:4570587-4570606 None:intergenic 55.0%
CACAGACACAAGTGTGGTGG+TGG + chr1_4:4570606-4570625 None:intergenic 55.0%
GGACACAGACACAAGTGTGG+TGG + chr1_4:4570609-4570628 None:intergenic 55.0%
TCCACCTTCACCGCAAAGCC+AGG - chr1_4:4569903-4569922 Msa0144990:three_prime_UTR 60.0%
!! ACCTGGCTTTGCGGTGAAGG+TGG + chr1_4:4569907-4569926 None:intergenic 60.0%
GGATTCCACCTTGTCCCTCC+TGG + chr1_4:4570371-4570390 None:intergenic 60.0%
!! AGTGTGGTGGTGGGAGTTCG+AGG + chr1_4:4570596-4570615 None:intergenic 60.0%
GGGGGACACAGACACAAGTG+TGG + chr1_4:4570612-4570631 None:intergenic 60.0%
TCCACCTTCACCGCAAAGCC+AGG - chr1_4:4569903-4569922 Msa0144990:three_prime_UTR 60.0%
!! ACCTGGCTTTGCGGTGAAGG+TGG + chr1_4:4569907-4569926 None:intergenic 60.0%
GGATTCCACCTTGTCCCTCC+TGG + chr1_4:4570371-4570390 None:intergenic 60.0%
!! AGTGTGGTGGTGGGAGTTCG+AGG + chr1_4:4570596-4570615 None:intergenic 60.0%
GGGGGACACAGACACAAGTG+TGG + chr1_4:4570612-4570631 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 4569635 4571488 4569635 ID=Msa0144990;Name=Msa0144990
chr1_4 mRNA 4569635 4571488 4569635 ID=Msa0144990-mRNA-1;Parent=Msa0144990;Name=Msa0144990-mRNA-1;_AED=0.07;_eAED=0.07;_QI=50|1|1|1|1|1|3|411|236
chr1_4 exon 4569635 4570198 4569635 ID=Msa0144990-mRNA-1:exon:1908;Parent=Msa0144990-mRNA-1
chr1_4 exon 4570453 4570770 4570453 ID=Msa0144990-mRNA-1:exon:1907;Parent=Msa0144990-mRNA-1
chr1_4 exon 4571199 4571488 4571199 ID=Msa0144990-mRNA-1:exon:1906;Parent=Msa0144990-mRNA-1
chr1_4 five_prime_UTR 4571439 4571488 4571439 ID=Msa0144990-mRNA-1:five_prime_utr;Parent=Msa0144990-mRNA-1
chr1_4 CDS 4571199 4571438 4571199 ID=Msa0144990-mRNA-1:cds;Parent=Msa0144990-mRNA-1
chr1_4 CDS 4570453 4570770 4570453 ID=Msa0144990-mRNA-1:cds;Parent=Msa0144990-mRNA-1
chr1_4 CDS 4570046 4570198 4570046 ID=Msa0144990-mRNA-1:cds;Parent=Msa0144990-mRNA-1
chr1_4 three_prime_UTR 4569635 4570045 4569635 ID=Msa0144990-mRNA-1:three_prime_utr;Parent=Msa0144990-mRNA-1
Gene Sequence

>Msa0144990

ATGAAAGTGCATCAATTCACACGTGGATTTTGGGAGCATGAACCTTCCCTCACACTTGGTTGCAAGCGTTTACGTCCTCTTGCACCTAAAATTTCTTCTAATAACACCGATAATGTTACTTCTAACACCCCTTCTCTTTCTTTTGATCTCAAGAGCTTCATTAGACCCGAAAGTGCTCCTAGAATTAGTCTTTCTTCCTCTGATGATAACAAGAAAGATCCACCTTCACCGCAAAGCCAGGTTGAAACGCATATTCCAGGAGGGACAAGGTGGAATCCAACACAAGAACAAATAGGAATATTGGAGATGTTATACAGAGGTGGAATGAGAACTCCAAATGCACAACAAATAGAACAGATCACAGTTCAACTTAGCAAGTACGGTAAAATTGAAGGGAAAAATGTGTTCTATTGGTTCCAAAACCACAAAGCACGCGAGAGACAAAAGCAGAAACGTAACAGTCTTGGCCTTCCTCATAGTCCTCGAACTCCCACCACCACACTTGTGTCTGTGTCCCCCACCTTTAGTACTATTACAACTTTGGACACCCCAAAAAGGGTGCAAGTAATGGAAAGAGATCAAGAAGATAGTCCATTGAAGAAGAGTAGGAGTTGGCCATTTGAGTACTTGGAAGAGAAAAATTGGTCAATGTGCAAAGTAGAGGAACATAAAACTCTAGAGCTTTTCCCATTACACCCAGAAGGCAGATGA
Protein sequence

>Msa0144990

MKVHQFTRGFWEHEPSLTLGCKRLRPLAPKISSNNTDNVTSNTPSLSFDLKSFIRPESAPRISLSSSDDNKKDPPSPQSQVETHIPGGTRWNPTQEQIGILEMLYRGGMRTPNAQQIEQITVQLSKYGKIEGKNVFYWFQNHKARERQKQKRNSLGLPHSPRTPTTTLVSVSPTFSTITTLDTPKRVQVMERDQEDSPLKKSRSWPFEYLEEKNWSMCKVEEHKTLELFPLHPEGR*