AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0004810

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0004810 MtrunA17_Chr1g0153171 98.305 236 4 0 1 236 1 236 1.06e-175 482
Msa0004810 MtrunA17_Chr8g0392991 45.255 137 69 1 22 152 22 158 2.24e-29 112
Msa0004810 MtrunA17_Chr7g0238431 71.642 67 19 0 86 152 4 70 5.78e-29 108
Msa0004810 MtrunA17_Chr4g0031341 36.932 176 81 4 90 235 25 200 1.81e-26 103
Msa0004810 MtrunA17_Chr5g0436661 65.152 66 23 0 90 155 33 98 8.85e-26 99.4
Msa0004810 MtrunA17_Chr4g0043891 68.750 64 20 0 89 152 7 70 1.13e-25 100
Msa0004810 MtrunA17_Chr5g0405351 64.062 64 22 1 89 151 29 92 1.20e-22 94.0
Msa0004810 MtrunA17_Chr3g0125111 40.146 137 60 2 22 152 22 142 6.47e-22 88.6
Msa0004810 MtrunA17_Chr8g0385441 76.923 52 12 0 101 152 93 144 2.76e-21 87.0
Msa0004810 MtrunA17_Chr2g0283141 35.577 104 55 1 49 152 26 117 3.87e-14 71.2
Msa0004810 MtrunA17_Chr7g0223471 46.774 62 33 0 90 151 47 108 1.68e-13 68.9
Msa0004810 MtrunA17_Chr3g0144021 39.474 76 46 0 90 165 82 157 3.20e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0004810 AT1G46480.1 52.896 259 91 9 1 236 1 251 1.63e-72 221
Msa0004810 AT1G46480.2 59.067 193 60 7 1 182 1 185 3.05e-63 196
Msa0004810 AT3G18010.1 42.857 147 74 2 6 152 2 138 6.13e-30 114
Msa0004810 AT3G11260.1 56.818 88 33 1 87 169 16 103 1.24e-27 104
Msa0004810 AT5G59340.1 61.333 75 29 0 78 152 2 76 5.75e-27 104
Msa0004810 AT2G28610.1 68.182 66 21 0 87 152 5 70 9.84e-27 103
Msa0004810 AT5G05770.1 63.077 65 24 0 90 154 29 93 1.72e-24 94.4
Msa0004810 AT2G17950.1 51.765 85 40 1 89 172 37 121 1.05e-23 96.7
Msa0004810 AT2G01500.1 66.667 63 19 1 84 144 53 115 2.07e-21 90.1
Msa0004810 AT2G33880.2 38.614 101 52 1 49 149 24 114 4.42e-14 71.2
Msa0004810 AT2G33880.1 38.614 101 52 1 49 149 24 114 4.70e-14 70.9
Msa0004810 AT1G20700.2 47.541 61 32 0 90 150 33 93 2.70e-13 65.9
Msa0004810 AT5G45980.1 46.774 62 33 0 90 151 55 116 4.97e-13 67.8
Msa0004810 AT1G20710.1 38.462 91 44 1 97 187 86 164 6.50e-13 65.9
Msa0004810 AT1G20700.3 47.541 61 32 0 90 150 95 155 9.15e-13 65.5
Msa0004810 AT1G20700.1 47.541 61 32 0 90 150 95 155 1.09e-12 65.5
Msa0004810 AT4G35550.1 47.541 61 32 0 90 150 99 159 1.15e-11 63.2

Find 71 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAATTCACACGTGGATTT+TGG 0.121803 1_1:-7267751 Msa0004810:CDS
ACAAATAAGTTTGGTTAATT+TGG 0.133382 1_1:+7266203 None:intergenic
AAGCAAATTAAAGCACATTT+TGG 0.180958 1_1:+7266335 None:intergenic
TCTCGCGTGCTTTGTGGTTT+TGG 0.203041 1_1:+7266922 None:intergenic
AAACTCCTTCATCTGCCTTC+TGG 0.237007 1_1:+7266387 None:intergenic
AACTCCTTCATCTGCCTTCT+GGG 0.285945 1_1:+7266388 None:intergenic
CAACTTTGGACACCCCAAAA+AGG 0.296385 1_1:-7266803 Msa0004810:CDS
ACTAATTCTAGGAGCACTTT+CGG 0.300053 1_1:+7267595 None:intergenic
TGTTCTATTTGTTGTGCATT+TGG 0.303503 1_1:+7267005 None:intergenic
CTAATTCTAGGAGCACTTTC+GGG 0.314910 1_1:+7267596 None:intergenic
ACAAGAACAAATAGGAATAT+TGG 0.328145 1_1:-7267057 Msa0004810:CDS
GAGGAAGAAAGACTAATTCT+AGG 0.342909 1_1:+7267584 None:intergenic
GGATTCCACCTTGTCCCTCC+TGG 0.366142 1_1:+7267083 None:intergenic
ATCTGCCTTCTGGGTGTAAT+GGG 0.376276 1_1:+7266397 None:intergenic
CTTTAGTACTATTACAACTT+TGG 0.384910 1_1:-7266817 Msa0004810:CDS
CATCTGCCTTCTGGGTGTAA+TGG 0.398760 1_1:+7266396 None:intergenic
ATTCCTATTTGTTCTTGTGT+TGG 0.415008 1_1:+7267062 None:intergenic
TTTCTTCTTTCATTTACATT+GGG 0.415761 1_1:-7266302 Msa0004810:three_prime_UTR
AATCCAACACAAGAACAAAT+AGG 0.426122 1_1:-7267065 Msa0004810:CDS
GTAATGTTATTCCATGACTT+TGG 0.426731 1_1:-7266228 Msa0004810:three_prime_UTR
TTCTCTTCCAAGTACTCAAA+TGG 0.427494 1_1:+7266468 None:intergenic
ATTTCTTCTTTCATTTACAT+TGG 0.443688 1_1:-7266303 Msa0004810:three_prime_UTR
TTTGTCTCTCGCGTGCTTTG+TGG 0.448508 1_1:+7266916 None:intergenic
TAGGTTGAAACGCATATTCC+AGG 0.450427 1_1:-7267101 Msa0004810:CDS
TCCACCTTCACCGCAAAGCC+AGG 0.461999 1_1:-7267543 Msa0004810:intron
TTTGTTGATTTCAGCGTAGT+AGG 0.464270 1_1:-7266166 Msa0004810:three_prime_UTR
GAGTTGGCCATTTGAGTACT+TGG 0.464745 1_1:-7266475 Msa0004810:CDS
GGTGTTAGAAGTAACATTAT+CGG 0.465029 1_1:+7267655 None:intergenic
CAGCGTAGTAGGTGTGACTT+TGG 0.468785 1_1:-7266155 Msa0004810:three_prime_UTR
CACAGACACAAGTGTGGTGG+TGG 0.469900 1_1:+7266848 None:intergenic
CAAAGTTGTAATAGTACTAA+AGG 0.472738 1_1:+7266818 None:intergenic
AAGATAAGATTAGTAGAAGT+AGG 0.473815 1_1:+7266040 None:intergenic
AGCAAGTACGGTAAAATTGA+AGG 0.477134 1_1:-7266966 Msa0004810:CDS
CAACTCCTACTCTTCTTCAA+TGG 0.501838 1_1:+7266492 None:intergenic
AAGCAGAAGCGTAACAGTCT+TGG 0.515335 1_1:-7266894 Msa0004810:CDS
AGTGTGGTGGTGGGAGTTCG+AGG 0.518160 1_1:+7266858 None:intergenic
CATGAACCTTCCCTCACACT+TGG 0.520457 1_1:-7267725 Msa0004810:CDS
TGAACCTGGCTTTGCGGTGA+AGG 0.521265 1_1:+7267539 None:intergenic
GTTGTAATAGTACTAAAGGT+GGG 0.528602 1_1:+7266822 None:intergenic
TGAGATCAAAAGAAAGAGAA+GGG 0.531578 1_1:+7267633 None:intergenic
TTAATTTGGCTCCAAAGTCA+TGG 0.533407 1_1:+7266217 None:intergenic
AGTATATGAACCTGGCTTTG+CGG 0.544738 1_1:+7267533 None:intergenic
ATAGTCCATTGAAGAAGAGT+AGG 0.545832 1_1:-7266497 Msa0004810:CDS
ATCTTTCTTGTTATCATCAG+AGG 0.556963 1_1:+7267565 None:intergenic
ACAGTTCAACTTAGCAAGTA+CGG 0.562866 1_1:-7266978 Msa0004810:CDS
CGCATATTCCAGGAGGGACA+AGG 0.565252 1_1:-7267091 Msa0004810:CDS
TACACCCAGAAGGCAGATGA+AGG 0.566613 1_1:-7266392 Msa0004810:exon
GTGGGAGTTCGAGGACTATG+AGG 0.567688 1_1:+7266867 None:intergenic
TTGGTCAATGTGCAAAGTAG+AGG 0.568050 1_1:-7266442 Msa0004810:CDS
TTGAGATCAAAAGAAAGAGA+AGG 0.574637 1_1:+7267632 None:intergenic
AGTTGTAATAGTACTAAAGG+TGG 0.581733 1_1:+7266821 None:intergenic
AACGCTTGCAACCAAGTGTG+AGG 0.582297 1_1:+7267714 None:intergenic
GCAAGTACGGTAAAATTGAA+GGG 0.588273 1_1:-7266965 Msa0004810:CDS
CATTGAAGAAGAGTAGGAGT+TGG 0.590517 1_1:-7266491 Msa0004810:CDS
ACCTGGCTTTGCGGTGAAGG+TGG 0.590968 1_1:+7267542 None:intergenic
TGTAATAGTACTAAAGGTGG+GGG 0.603492 1_1:+7266824 None:intergenic
TTGTAATAGTACTAAAGGTG+GGG 0.606911 1_1:+7266823 None:intergenic
TTGGAGATGTTATACAGAGG+TGG 0.611718 1_1:-7267038 Msa0004810:CDS
GAGTTCGAGGACTATGAGGA+AGG 0.633348 1_1:+7266871 None:intergenic
GATTAGTAGAAGTAGGTGCG+TGG 0.641260 1_1:+7266047 None:intergenic
TATTTCTTCATCAGACCGTG+TGG 0.650611 1_1:-7267811 Msa0004810:five_prime_UTR
ACGCTTGCAACCAAGTGTGA+GGG 0.656705 1_1:+7267715 None:intergenic
TTGAAACGCATATTCCAGGA+GGG 0.657126 1_1:-7267097 Msa0004810:CDS
ACAGACACAAGTGTGGTGGT+GGG 0.659851 1_1:+7266849 None:intergenic
GGACACAGACACAAGTGTGG+TGG 0.666759 1_1:+7266845 None:intergenic
GAGATCAAAAGAAAGAGAAG+GGG 0.671823 1_1:+7267634 None:intergenic
TTGCAACCAAGTGTGAGGGA+AGG 0.674259 1_1:+7267719 None:intergenic
GTTGAAACGCATATTCCAGG+AGG 0.708848 1_1:-7267098 Msa0004810:CDS
ATATTGGAGATGTTATACAG+AGG 0.712720 1_1:-7267041 Msa0004810:CDS
AAAGTGCATCAATTCACACG+TGG 0.742454 1_1:-7267758 Msa0004810:CDS
ATATTCCAGGAGGGACAAGG+TGG 0.756182 1_1:-7267088 Msa0004810:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATACTAATAAAGTATTT+TGG - chr1_1:7266465-7266484 Msa0004810:CDS 10.0%
!!! ATAATACTAATAAAGTATTT+TGG - chr1_1:7266465-7266484 Msa0004810:CDS 10.0%
!! CAGACTTAATTAAAAAAAAA+AGG + chr1_1:7266309-7266328 None:intergenic 15.0%
!!! TCTTTTAAAAACTTTTGATT+TGG - chr1_1:7266625-7266644 Msa0004810:intron 15.0%
!! AAATCAACAAATGAAAAAAA+AGG + chr1_1:7267620-7267639 None:intergenic 15.0%
!! CAGACTTAATTAAAAAAAAA+AGG + chr1_1:7266309-7266328 None:intergenic 15.0%
!!! TCTTTTAAAAACTTTTGATT+TGG - chr1_1:7266625-7266644 Msa0004810:intron 15.0%
!! AAATCAACAAATGAAAAAAA+AGG + chr1_1:7267620-7267639 None:intergenic 15.0%
!!! GTTTAATATAATTTTCCACA+CGG + chr1_1:7266002-7266021 None:intergenic 20.0%
!!! GTGTTATTAGAAGAAATTTT+AGG + chr1_1:7266121-7266140 None:intergenic 20.0%
!! ATTAACTTCATGTAACATAT+AGG - chr1_1:7266422-7266441 Msa0004810:CDS 20.0%
!! TTAACTTCATGTAACATATA+GGG - chr1_1:7266423-7266442 Msa0004810:CDS 20.0%
!!! ATATAGATACATCTGAAATT+CGG + chr1_1:7266510-7266529 None:intergenic 20.0%
!! TCTATTATTTGTGTATGTAT+AGG - chr1_1:7266675-7266694 Msa0004810:intron 20.0%
!!! TTTTTGATTAAAAGGAAAGT+AGG - chr1_1:7267140-7267159 Msa0004810:intron 20.0%
!!! TTGTTCATGGTTTTAAATTA+CGG + chr1_1:7267167-7267186 None:intergenic 20.0%
!! AATTAGTATACTATTGTTCA+TGG + chr1_1:7267180-7267199 None:intergenic 20.0%
!! AATACTATAATCTTGTTACT+AGG - chr1_1:7267202-7267221 Msa0004810:intron 20.0%
!!! TCGAATATGTTACATTTTTT+TGG - chr1_1:7267243-7267262 Msa0004810:intron 20.0%
!! ATTTCTTCTTTCATTTACAT+TGG - chr1_1:7267492-7267511 Msa0004810:intron 20.0%
!! TTTCTTCTTTCATTTACATT+GGG - chr1_1:7267493-7267512 Msa0004810:intron 20.0%
!! ACAAATAAGTTTGGTTAATT+TGG + chr1_1:7267595-7267614 None:intergenic 20.0%
!!! ATACTATACTCTCATATTTT+AGG - chr1_1:7267793-7267812 Msa0004810:five_prime_UTR 20.0%
!!! GTTTAATATAATTTTCCACA+CGG + chr1_1:7266002-7266021 None:intergenic 20.0%
!!! GTGTTATTAGAAGAAATTTT+AGG + chr1_1:7266121-7266140 None:intergenic 20.0%
!! ATTAACTTCATGTAACATAT+AGG - chr1_1:7266422-7266441 Msa0004810:CDS 20.0%
!! TTAACTTCATGTAACATATA+GGG - chr1_1:7266423-7266442 Msa0004810:CDS 20.0%
!!! ATATAGATACATCTGAAATT+CGG + chr1_1:7266510-7266529 None:intergenic 20.0%
!! TCTATTATTTGTGTATGTAT+AGG - chr1_1:7266675-7266694 Msa0004810:intron 20.0%
!!! TTTTTGATTAAAAGGAAAGT+AGG - chr1_1:7267140-7267159 Msa0004810:intron 20.0%
!!! TTGTTCATGGTTTTAAATTA+CGG + chr1_1:7267167-7267186 None:intergenic 20.0%
!! AATTAGTATACTATTGTTCA+TGG + chr1_1:7267180-7267199 None:intergenic 20.0%
!! AATACTATAATCTTGTTACT+AGG - chr1_1:7267202-7267221 Msa0004810:intron 20.0%
!!! TCGAATATGTTACATTTTTT+TGG - chr1_1:7267243-7267262 Msa0004810:intron 20.0%
!! ATTTCTTCTTTCATTTACAT+TGG - chr1_1:7267492-7267511 Msa0004810:intron 20.0%
!! TTTCTTCTTTCATTTACATT+GGG - chr1_1:7267493-7267512 Msa0004810:intron 20.0%
!! ACAAATAAGTTTGGTTAATT+TGG + chr1_1:7267595-7267614 None:intergenic 20.0%
!!! ATACTATACTCTCATATTTT+AGG - chr1_1:7267793-7267812 Msa0004810:five_prime_UTR 20.0%
!!! ACTAATAAAGTATTTTGGTC+CGG - chr1_1:7266470-7266489 Msa0004810:CDS 25.0%
! ACAAGAACAAATAGGAATAT+TGG - chr1_1:7266738-7266757 Msa0004810:intron 25.0%
! CAAAGTTGTAATAGTACTAA+AGG + chr1_1:7266980-7266999 None:intergenic 25.0%
! CTTTAGTACTATTACAACTT+TGG - chr1_1:7266978-7266997 Msa0004810:CDS 25.0%
!! AATATGAACATACCCTTTTT+GGG + chr1_1:7267008-7267027 None:intergenic 25.0%
! TCAACTTAAGCTATATATTG+TGG - chr1_1:7267038-7267057 Msa0004810:CDS 25.0%
!! CGACAATATAAAGGATTTTA+AGG + chr1_1:7267086-7267105 None:intergenic 25.0%
!!! TATGTTACATTTTTTTGGAC+AGG - chr1_1:7267248-7267267 Msa0004810:intron 25.0%
!! AAGCAAATTAAAGCACATTT+TGG + chr1_1:7267463-7267482 None:intergenic 25.0%
! AAAAAGGTGACAAATAAGTT+TGG + chr1_1:7267604-7267623 None:intergenic 25.0%
! AAGATAAGATTAGTAGAAGT+AGG + chr1_1:7267758-7267777 None:intergenic 25.0%
!!! ACTAATAAAGTATTTTGGTC+CGG - chr1_1:7266470-7266489 Msa0004810:CDS 25.0%
! ACAAGAACAAATAGGAATAT+TGG - chr1_1:7266738-7266757 Msa0004810:intron 25.0%
! CAAAGTTGTAATAGTACTAA+AGG + chr1_1:7266980-7266999 None:intergenic 25.0%
! CTTTAGTACTATTACAACTT+TGG - chr1_1:7266978-7266997 Msa0004810:CDS 25.0%
!! AATATGAACATACCCTTTTT+GGG + chr1_1:7267008-7267027 None:intergenic 25.0%
! TCAACTTAAGCTATATATTG+TGG - chr1_1:7267038-7267057 Msa0004810:CDS 25.0%
!! CGACAATATAAAGGATTTTA+AGG + chr1_1:7267086-7267105 None:intergenic 25.0%
!!! TATGTTACATTTTTTTGGAC+AGG - chr1_1:7267248-7267267 Msa0004810:intron 25.0%
!! AAGCAAATTAAAGCACATTT+TGG + chr1_1:7267463-7267482 None:intergenic 25.0%
! AAAAAGGTGACAAATAAGTT+TGG + chr1_1:7267604-7267623 None:intergenic 25.0%
! AAGATAAGATTAGTAGAAGT+AGG + chr1_1:7267758-7267777 None:intergenic 25.0%
GGTGTTAGAAGTAACATTAT+CGG + chr1_1:7266143-7266162 None:intergenic 30.0%
TGAGATCAAAAGAAAGAGAA+GGG + chr1_1:7266165-7266184 None:intergenic 30.0%
TTGAGATCAAAAGAAAGAGA+AGG + chr1_1:7266166-7266185 None:intergenic 30.0%
! ATCTTTCTTGTTATCATCAG+AGG + chr1_1:7266233-7266252 None:intergenic 30.0%
!!! CTAATAAAGTATTTTGGTCC+GGG - chr1_1:7266471-7266490 Msa0004810:CDS 30.0%
AATCCAACACAAGAACAAAT+AGG - chr1_1:7266730-7266749 Msa0004810:intron 30.0%
ATTCCTATTTGTTCTTGTGT+TGG + chr1_1:7266736-7266755 None:intergenic 30.0%
ATATTGGAGATGTTATACAG+AGG - chr1_1:7266754-7266773 Msa0004810:intron 30.0%
!! TGTTCTATTTGTTGTGCATT+TGG + chr1_1:7266793-7266812 None:intergenic 30.0%
AAGGGAAAAATGTGTTCTAT+TGG - chr1_1:7266848-7266867 Msa0004810:CDS 30.0%
TTGTAATAGTACTAAAGGTG+GGG + chr1_1:7266975-7266994 None:intergenic 30.0%
GTTGTAATAGTACTAAAGGT+GGG + chr1_1:7266976-7266995 None:intergenic 30.0%
AGTTGTAATAGTACTAAAGG+TGG + chr1_1:7266977-7266996 None:intergenic 30.0%
! ATATGAACATACCCTTTTTG+GGG + chr1_1:7267007-7267026 None:intergenic 30.0%
! GAATATGAACATACCCTTTT+TGG + chr1_1:7267009-7267028 None:intergenic 30.0%
AGTACTTGGAAGAGAAAAAT+TGG - chr1_1:7267334-7267353 Msa0004810:intron 30.0%
GTAATGTTATTCCATGACTT+TGG - chr1_1:7267567-7267586 Msa0004810:CDS 30.0%
AAGCTTATGTCGTCTAAAAA+AGG + chr1_1:7267677-7267696 None:intergenic 30.0%
!!! TCGAAAGCTTTTAGGTTAAA+AGG - chr1_1:7267702-7267721 Msa0004810:CDS 30.0%
GGTGTTAGAAGTAACATTAT+CGG + chr1_1:7266143-7266162 None:intergenic 30.0%
TGAGATCAAAAGAAAGAGAA+GGG + chr1_1:7266165-7266184 None:intergenic 30.0%
TTGAGATCAAAAGAAAGAGA+AGG + chr1_1:7266166-7266185 None:intergenic 30.0%
! ATCTTTCTTGTTATCATCAG+AGG + chr1_1:7266233-7266252 None:intergenic 30.0%
!!! CTAATAAAGTATTTTGGTCC+GGG - chr1_1:7266471-7266490 Msa0004810:CDS 30.0%
AATCCAACACAAGAACAAAT+AGG - chr1_1:7266730-7266749 Msa0004810:intron 30.0%
ATTCCTATTTGTTCTTGTGT+TGG + chr1_1:7266736-7266755 None:intergenic 30.0%
ATATTGGAGATGTTATACAG+AGG - chr1_1:7266754-7266773 Msa0004810:intron 30.0%
!! TGTTCTATTTGTTGTGCATT+TGG + chr1_1:7266793-7266812 None:intergenic 30.0%
AAGGGAAAAATGTGTTCTAT+TGG - chr1_1:7266848-7266867 Msa0004810:CDS 30.0%
TTGTAATAGTACTAAAGGTG+GGG + chr1_1:7266975-7266994 None:intergenic 30.0%
GTTGTAATAGTACTAAAGGT+GGG + chr1_1:7266976-7266995 None:intergenic 30.0%
AGTTGTAATAGTACTAAAGG+TGG + chr1_1:7266977-7266996 None:intergenic 30.0%
! ATATGAACATACCCTTTTTG+GGG + chr1_1:7267007-7267026 None:intergenic 30.0%
! GAATATGAACATACCCTTTT+TGG + chr1_1:7267009-7267028 None:intergenic 30.0%
AGTACTTGGAAGAGAAAAAT+TGG - chr1_1:7267334-7267353 Msa0004810:intron 30.0%
GTAATGTTATTCCATGACTT+TGG - chr1_1:7267567-7267586 Msa0004810:CDS 30.0%
AAGCTTATGTCGTCTAAAAA+AGG + chr1_1:7267677-7267696 None:intergenic 30.0%
!!! TCGAAAGCTTTTAGGTTAAA+AGG - chr1_1:7267702-7267721 Msa0004810:CDS 30.0%
! ATCAATTCACACGTGGATTT+TGG - chr1_1:7266044-7266063 Msa0004810:three_prime_UTR 35.0%
! TCAATTCACACGTGGATTTT+GGG - chr1_1:7266045-7266064 Msa0004810:three_prime_UTR 35.0%
!! GAAGAAATTTTAGGTGCAAG+AGG + chr1_1:7266112-7266131 None:intergenic 35.0%
GAGATCAAAAGAAAGAGAAG+GGG + chr1_1:7266164-7266183 None:intergenic 35.0%
!! ACTAATTCTAGGAGCACTTT+CGG + chr1_1:7266203-7266222 None:intergenic 35.0%
GAGGAAGAAAGACTAATTCT+AGG + chr1_1:7266214-7266233 None:intergenic 35.0%
TAGAGCTTAGTATATGAACC+TGG + chr1_1:7266273-7266292 None:intergenic 35.0%
AATTATACCACTCTACTGCA+TGG + chr1_1:7266337-7266356 None:intergenic 35.0%
! TTCGGTTCAACAATTTTTCC+CGG + chr1_1:7266492-7266511 None:intergenic 35.0%
ACAGTTCAACTTAGCAAGTA+CGG - chr1_1:7266817-7266836 Msa0004810:CDS 35.0%
AGCAAGTACGGTAAAATTGA+AGG - chr1_1:7266829-7266848 Msa0004810:CDS 35.0%
GCAAGTACGGTAAAATTGAA+GGG - chr1_1:7266830-7266849 Msa0004810:CDS 35.0%
TGTAATAGTACTAAAGGTGG+GGG + chr1_1:7266974-7266993 None:intergenic 35.0%
! TAGTTGCGTCGACAATATAA+AGG + chr1_1:7267095-7267114 None:intergenic 35.0%
ATAGTCCATTGAAGAAGAGT+AGG - chr1_1:7267298-7267317 Msa0004810:intron 35.0%
TTCTCTTCCAAGTACTCAAA+TGG + chr1_1:7267330-7267349 None:intergenic 35.0%
! TTAATTTGGCTCCAAAGTCA+TGG + chr1_1:7267581-7267600 None:intergenic 35.0%
!! TTTGTTGATTTCAGCGTAGT+AGG - chr1_1:7267629-7267648 Msa0004810:CDS 35.0%
! CTTGTGAATCGAAAGCTTTT+AGG - chr1_1:7267694-7267713 Msa0004810:CDS 35.0%
! ATCAATTCACACGTGGATTT+TGG - chr1_1:7266044-7266063 Msa0004810:three_prime_UTR 35.0%
! TCAATTCACACGTGGATTTT+GGG - chr1_1:7266045-7266064 Msa0004810:three_prime_UTR 35.0%
!! GAAGAAATTTTAGGTGCAAG+AGG + chr1_1:7266112-7266131 None:intergenic 35.0%
GAGATCAAAAGAAAGAGAAG+GGG + chr1_1:7266164-7266183 None:intergenic 35.0%
!! ACTAATTCTAGGAGCACTTT+CGG + chr1_1:7266203-7266222 None:intergenic 35.0%
GAGGAAGAAAGACTAATTCT+AGG + chr1_1:7266214-7266233 None:intergenic 35.0%
TAGAGCTTAGTATATGAACC+TGG + chr1_1:7266273-7266292 None:intergenic 35.0%
AATTATACCACTCTACTGCA+TGG + chr1_1:7266337-7266356 None:intergenic 35.0%
! TTCGGTTCAACAATTTTTCC+CGG + chr1_1:7266492-7266511 None:intergenic 35.0%
ACAGTTCAACTTAGCAAGTA+CGG - chr1_1:7266817-7266836 Msa0004810:CDS 35.0%
AGCAAGTACGGTAAAATTGA+AGG - chr1_1:7266829-7266848 Msa0004810:CDS 35.0%
GCAAGTACGGTAAAATTGAA+GGG - chr1_1:7266830-7266849 Msa0004810:CDS 35.0%
TGTAATAGTACTAAAGGTGG+GGG + chr1_1:7266974-7266993 None:intergenic 35.0%
! TAGTTGCGTCGACAATATAA+AGG + chr1_1:7267095-7267114 None:intergenic 35.0%
ATAGTCCATTGAAGAAGAGT+AGG - chr1_1:7267298-7267317 Msa0004810:intron 35.0%
TTCTCTTCCAAGTACTCAAA+TGG + chr1_1:7267330-7267349 None:intergenic 35.0%
! TTAATTTGGCTCCAAAGTCA+TGG + chr1_1:7267581-7267600 None:intergenic 35.0%
!! TTTGTTGATTTCAGCGTAGT+AGG - chr1_1:7267629-7267648 Msa0004810:CDS 35.0%
! CTTGTGAATCGAAAGCTTTT+AGG - chr1_1:7267694-7267713 Msa0004810:CDS 35.0%
TATTTCTTCATCAGACCGTG+TGG - chr1_1:7265984-7266003 Msa0004810:three_prime_UTR 40.0%
AAAGTGCATCAATTCACACG+TGG - chr1_1:7266037-7266056 Msa0004810:three_prime_UTR 40.0%
!! CTAATTCTAGGAGCACTTTC+GGG + chr1_1:7266202-7266221 None:intergenic 40.0%
!! AGTATATGAACCTGGCTTTG+CGG + chr1_1:7266265-7266284 None:intergenic 40.0%
CGAGTCTCATATGGAAAAAG+AGG + chr1_1:7266565-7266584 None:intergenic 40.0%
AGTAACTCTCGAGTCTCATA+TGG + chr1_1:7266574-7266593 None:intergenic 40.0%
! TAGGTTGAAACGCATATTCC+AGG - chr1_1:7266694-7266713 Msa0004810:intron 40.0%
TTGAAACGCATATTCCAGGA+GGG - chr1_1:7266698-7266717 Msa0004810:intron 40.0%
TTGGAGATGTTATACAGAGG+TGG - chr1_1:7266757-7266776 Msa0004810:intron 40.0%
AACTTTGGACACCCCAAAAA+GGG - chr1_1:7266993-7267012 Msa0004810:CDS 40.0%
! TTTTGGACAGGTGCAAGTAA+TGG - chr1_1:7267260-7267279 Msa0004810:intron 40.0%
CAACTCCTACTCTTCTTCAA+TGG + chr1_1:7267306-7267325 None:intergenic 40.0%
CATTGAAGAAGAGTAGGAGT+TGG - chr1_1:7267304-7267323 Msa0004810:intron 40.0%
TTGGTCAATGTGCAAAGTAG+AGG - chr1_1:7267353-7267372 Msa0004810:intron 40.0%
TATTTCTTCATCAGACCGTG+TGG - chr1_1:7265984-7266003 Msa0004810:three_prime_UTR 40.0%
AAAGTGCATCAATTCACACG+TGG - chr1_1:7266037-7266056 Msa0004810:three_prime_UTR 40.0%
!! CTAATTCTAGGAGCACTTTC+GGG + chr1_1:7266202-7266221 None:intergenic 40.0%
!! AGTATATGAACCTGGCTTTG+CGG + chr1_1:7266265-7266284 None:intergenic 40.0%
CGAGTCTCATATGGAAAAAG+AGG + chr1_1:7266565-7266584 None:intergenic 40.0%
AGTAACTCTCGAGTCTCATA+TGG + chr1_1:7266574-7266593 None:intergenic 40.0%
! TAGGTTGAAACGCATATTCC+AGG - chr1_1:7266694-7266713 Msa0004810:intron 40.0%
TTGAAACGCATATTCCAGGA+GGG - chr1_1:7266698-7266717 Msa0004810:intron 40.0%
TTGGAGATGTTATACAGAGG+TGG - chr1_1:7266757-7266776 Msa0004810:intron 40.0%
AACTTTGGACACCCCAAAAA+GGG - chr1_1:7266993-7267012 Msa0004810:CDS 40.0%
! TTTTGGACAGGTGCAAGTAA+TGG - chr1_1:7267260-7267279 Msa0004810:intron 40.0%
CAACTCCTACTCTTCTTCAA+TGG + chr1_1:7267306-7267325 None:intergenic 40.0%
CATTGAAGAAGAGTAGGAGT+TGG - chr1_1:7267304-7267323 Msa0004810:intron 40.0%
TTGGTCAATGTGCAAAGTAG+AGG - chr1_1:7267353-7267372 Msa0004810:intron 40.0%
ATACCACTCTACTGCATGGA+TGG + chr1_1:7266333-7266352 None:intergenic 45.0%
GTTGAAACGCATATTCCAGG+AGG - chr1_1:7266697-7266716 Msa0004810:intron 45.0%
! AAGCAGAAGCGTAACAGTCT+TGG - chr1_1:7266901-7266920 Msa0004810:CDS 45.0%
CAACTTTGGACACCCCAAAA+AGG - chr1_1:7266992-7267011 Msa0004810:CDS 45.0%
AGGTCTCTGCAACGACATTT+CGG + chr1_1:7267066-7267085 None:intergenic 45.0%
TGTCGACGCAACTATAGTCT+TGG - chr1_1:7267101-7267120 Msa0004810:CDS 45.0%
GAGTTGGCCATTTGAGTACT+TGG - chr1_1:7267320-7267339 Msa0004810:intron 45.0%
! CTTTTCCCATTACACCCAGA+AGG - chr1_1:7267393-7267412 Msa0004810:intron 45.0%
ATCTGCCTTCTGGGTGTAAT+GGG + chr1_1:7267401-7267420 None:intergenic 45.0%
AACTCCTTCATCTGCCTTCT+GGG + chr1_1:7267410-7267429 None:intergenic 45.0%
AAACTCCTTCATCTGCCTTC+TGG + chr1_1:7267411-7267430 None:intergenic 45.0%
GATTAGTAGAAGTAGGTGCG+TGG + chr1_1:7267751-7267770 None:intergenic 45.0%
ATACCACTCTACTGCATGGA+TGG + chr1_1:7266333-7266352 None:intergenic 45.0%
GTTGAAACGCATATTCCAGG+AGG - chr1_1:7266697-7266716 Msa0004810:intron 45.0%
! AAGCAGAAGCGTAACAGTCT+TGG - chr1_1:7266901-7266920 Msa0004810:CDS 45.0%
CAACTTTGGACACCCCAAAA+AGG - chr1_1:7266992-7267011 Msa0004810:CDS 45.0%
AGGTCTCTGCAACGACATTT+CGG + chr1_1:7267066-7267085 None:intergenic 45.0%
TGTCGACGCAACTATAGTCT+TGG - chr1_1:7267101-7267120 Msa0004810:CDS 45.0%
GAGTTGGCCATTTGAGTACT+TGG - chr1_1:7267320-7267339 Msa0004810:intron 45.0%
! CTTTTCCCATTACACCCAGA+AGG - chr1_1:7267393-7267412 Msa0004810:intron 45.0%
ATCTGCCTTCTGGGTGTAAT+GGG + chr1_1:7267401-7267420 None:intergenic 45.0%
AACTCCTTCATCTGCCTTCT+GGG + chr1_1:7267410-7267429 None:intergenic 45.0%
AAACTCCTTCATCTGCCTTC+TGG + chr1_1:7267411-7267430 None:intergenic 45.0%
GATTAGTAGAAGTAGGTGCG+TGG + chr1_1:7267751-7267770 None:intergenic 45.0%
!! ATCAAAAAAAAAAAAAATTA+CGG + chr1_1:7267128-7267147 None:intergenic 5.0%
!!! TTTTTTTTTTTTTGATTAAA+AGG - chr1_1:7267132-7267151 Msa0004810:intron 5.0%
!! ATCAAAAAAAAAAAAAATTA+CGG + chr1_1:7267128-7267147 None:intergenic 5.0%
!!! TTTTTTTTTTTTTGATTAAA+AGG - chr1_1:7267132-7267151 Msa0004810:intron 5.0%
CATGAACCTTCCCTCACACT+TGG - chr1_1:7266070-7266089 Msa0004810:three_prime_UTR 50.0%
TTGCAACCAAGTGTGAGGGA+AGG + chr1_1:7266079-7266098 None:intergenic 50.0%
ACGCTTGCAACCAAGTGTGA+GGG + chr1_1:7266083-7266102 None:intergenic 50.0%
AACGCTTGCAACCAAGTGTG+AGG + chr1_1:7266084-7266103 None:intergenic 50.0%
TGTCCATCCATGCAGTAGAG+TGG - chr1_1:7266327-7266346 Msa0004810:three_prime_UTR 50.0%
ATATTCCAGGAGGGACAAGG+TGG - chr1_1:7266707-7266726 Msa0004810:intron 50.0%
! TCTCGCGTGCTTTGTGGTTT+TGG + chr1_1:7266876-7266895 None:intergenic 50.0%
TTTGTCTCTCGCGTGCTTTG+TGG + chr1_1:7266882-7266901 None:intergenic 50.0%
GAGTTCGAGGACTATGAGGA+AGG + chr1_1:7266927-7266946 None:intergenic 50.0%
ACAGACACAAGTGTGGTGGT+GGG + chr1_1:7266949-7266968 None:intergenic 50.0%
CATCTGCCTTCTGGGTGTAA+TGG + chr1_1:7267402-7267421 None:intergenic 50.0%
TACACCCAGAAGGCAGATGA+AGG - chr1_1:7267403-7267422 Msa0004810:intron 50.0%
CAGCGTAGTAGGTGTGACTT+TGG - chr1_1:7267640-7267659 Msa0004810:CDS 50.0%
CATGAACCTTCCCTCACACT+TGG - chr1_1:7266070-7266089 Msa0004810:three_prime_UTR 50.0%
TTGCAACCAAGTGTGAGGGA+AGG + chr1_1:7266079-7266098 None:intergenic 50.0%
ACGCTTGCAACCAAGTGTGA+GGG + chr1_1:7266083-7266102 None:intergenic 50.0%
AACGCTTGCAACCAAGTGTG+AGG + chr1_1:7266084-7266103 None:intergenic 50.0%
TGTCCATCCATGCAGTAGAG+TGG - chr1_1:7266327-7266346 Msa0004810:three_prime_UTR 50.0%
ATATTCCAGGAGGGACAAGG+TGG - chr1_1:7266707-7266726 Msa0004810:intron 50.0%
! TCTCGCGTGCTTTGTGGTTT+TGG + chr1_1:7266876-7266895 None:intergenic 50.0%
TTTGTCTCTCGCGTGCTTTG+TGG + chr1_1:7266882-7266901 None:intergenic 50.0%
GAGTTCGAGGACTATGAGGA+AGG + chr1_1:7266927-7266946 None:intergenic 50.0%
ACAGACACAAGTGTGGTGGT+GGG + chr1_1:7266949-7266968 None:intergenic 50.0%
CATCTGCCTTCTGGGTGTAA+TGG + chr1_1:7267402-7267421 None:intergenic 50.0%
TACACCCAGAAGGCAGATGA+AGG - chr1_1:7267403-7267422 Msa0004810:intron 50.0%
CAGCGTAGTAGGTGTGACTT+TGG - chr1_1:7267640-7267659 Msa0004810:CDS 50.0%
!! TGAACCTGGCTTTGCGGTGA+AGG + chr1_1:7266259-7266278 None:intergenic 55.0%
CGCATATTCCAGGAGGGACA+AGG - chr1_1:7266704-7266723 Msa0004810:intron 55.0%
GTGGGAGTTCGAGGACTATG+AGG + chr1_1:7266931-7266950 None:intergenic 55.0%
CACAGACACAAGTGTGGTGG+TGG + chr1_1:7266950-7266969 None:intergenic 55.0%
GGACACAGACACAAGTGTGG+TGG + chr1_1:7266953-7266972 None:intergenic 55.0%
!! TGAACCTGGCTTTGCGGTGA+AGG + chr1_1:7266259-7266278 None:intergenic 55.0%
CGCATATTCCAGGAGGGACA+AGG - chr1_1:7266704-7266723 Msa0004810:intron 55.0%
GTGGGAGTTCGAGGACTATG+AGG + chr1_1:7266931-7266950 None:intergenic 55.0%
CACAGACACAAGTGTGGTGG+TGG + chr1_1:7266950-7266969 None:intergenic 55.0%
GGACACAGACACAAGTGTGG+TGG + chr1_1:7266953-7266972 None:intergenic 55.0%
TCCACCTTCACCGCAAAGCC+AGG - chr1_1:7266252-7266271 Msa0004810:three_prime_UTR 60.0%
!! ACCTGGCTTTGCGGTGAAGG+TGG + chr1_1:7266256-7266275 None:intergenic 60.0%
GGATTCCACCTTGTCCCTCC+TGG + chr1_1:7266715-7266734 None:intergenic 60.0%
!! AGTGTGGTGGTGGGAGTTCG+AGG + chr1_1:7266940-7266959 None:intergenic 60.0%
GGGGGACACAGACACAAGTG+TGG + chr1_1:7266956-7266975 None:intergenic 60.0%
TCCACCTTCACCGCAAAGCC+AGG - chr1_1:7266252-7266271 Msa0004810:three_prime_UTR 60.0%
!! ACCTGGCTTTGCGGTGAAGG+TGG + chr1_1:7266256-7266275 None:intergenic 60.0%
GGATTCCACCTTGTCCCTCC+TGG + chr1_1:7266715-7266734 None:intergenic 60.0%
!! AGTGTGGTGGTGGGAGTTCG+AGG + chr1_1:7266940-7266959 None:intergenic 60.0%
GGGGGACACAGACACAAGTG+TGG + chr1_1:7266956-7266975 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 7265984 7267833 7265984 ID=Msa0004810;Name=Msa0004810
chr1_1 mRNA 7265984 7267833 7265984 ID=Msa0004810-mRNA-1;Parent=Msa0004810;Name=Msa0004810-mRNA-1;_AED=0.06;_eAED=0.06;_QI=50|1|1|1|1|1|3|411|236
chr1_1 exon 7265984 7266547 7265984 ID=Msa0004810-mRNA-1:exon:2529;Parent=Msa0004810-mRNA-1
chr1_1 exon 7266803 7267120 7266803 ID=Msa0004810-mRNA-1:exon:2528;Parent=Msa0004810-mRNA-1
chr1_1 exon 7267544 7267833 7267544 ID=Msa0004810-mRNA-1:exon:2527;Parent=Msa0004810-mRNA-1
chr1_1 five_prime_UTR 7267784 7267833 7267784 ID=Msa0004810-mRNA-1:five_prime_utr;Parent=Msa0004810-mRNA-1
chr1_1 CDS 7267544 7267783 7267544 ID=Msa0004810-mRNA-1:cds;Parent=Msa0004810-mRNA-1
chr1_1 CDS 7266803 7267120 7266803 ID=Msa0004810-mRNA-1:cds;Parent=Msa0004810-mRNA-1
chr1_1 CDS 7266395 7266547 7266395 ID=Msa0004810-mRNA-1:cds;Parent=Msa0004810-mRNA-1
chr1_1 three_prime_UTR 7265984 7266394 7265984 ID=Msa0004810-mRNA-1:three_prime_utr;Parent=Msa0004810-mRNA-1
Gene Sequence

>Msa0004810

ATGAAAGTGCATCAATTCACACGTGGATTTTGGGAGCATGAACCTTCCCTCACACTTGGTTGCAAGCGTTTACGTCCTCTTGCACCTAAAATTTCTTCTAATAACACCGATAATGTTACTTCTAACACCCCTTCTCTTTCTTTTGATCTCAAGAGCTTCATTAGACCCGAAAGTGCTCCTAGAATTAGTCTTTCTTCCTCTGATGATAACAAGAAAGATCCACCTTCACCGCAAAGCCAGGTTGAAACGCATATTCCAGGAGGGACAAGGTGGAATCCAACACAAGAACAAATAGGAATATTGGAGATGTTATACAGAGGTGGAATGAGAACTCCAAATGCACAACAAATAGAACAGATCACAGTTCAACTTAGCAAGTACGGTAAAATTGAAGGGAAAAATGTGTTCTATTGGTTCCAAAACCACAAAGCACGCGAGAGACAAAAGCAGAAGCGTAACAGTCTTGGCCTTCCTCATAGTCCTCGAACTCCCACCACCACACTTGTGTCTGTGTCCCCCACCTTTAGTACTATTACAACTTTGGACACCCCAAAAAGGGTGCAAGTAATGGAAAGAGATCAAGAAGATAGTCCATTGAAGAAGAGTAGGAGTTGGCCATTTGAGTACTTGGAAGAGAAAAATTGGTCAATGTGCAAAGTAGAGGAACATAAAACTCTAGAGCTTTTCCCATTACACCCAGAAGGCAGATGA
Protein sequence

>Msa0004810

MKVHQFTRGFWEHEPSLTLGCKRLRPLAPKISSNNTDNVTSNTPSLSFDLKSFIRPESAPRISLSSSDDNKKDPPSPQSQVETHIPGGTRWNPTQEQIGILEMLYRGGMRTPNAQQIEQITVQLSKYGKIEGKNVFYWFQNHKARERQKQKRNSLGLPHSPRTPTTTLVSVSPTFSTITTLDTPKRVQVMERDQEDSPLKKSRSWPFEYLEEKNWSMCKVEEHKTLELFPLHPEGR*