AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0096960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0096960 MtrunA17_Chr1g0153171 98.305 236 4 0 1 236 1 236 1.06e-175 482
Msa0096960 MtrunA17_Chr8g0392991 45.255 137 69 1 22 152 22 158 2.24e-29 112
Msa0096960 MtrunA17_Chr7g0238431 71.642 67 19 0 86 152 4 70 5.78e-29 108
Msa0096960 MtrunA17_Chr4g0031341 36.932 176 81 4 90 235 25 200 1.81e-26 103
Msa0096960 MtrunA17_Chr5g0436661 65.152 66 23 0 90 155 33 98 8.85e-26 99.4
Msa0096960 MtrunA17_Chr4g0043891 68.750 64 20 0 89 152 7 70 1.13e-25 100
Msa0096960 MtrunA17_Chr5g0405351 64.062 64 22 1 89 151 29 92 1.20e-22 94.0
Msa0096960 MtrunA17_Chr3g0125111 40.146 137 60 2 22 152 22 142 6.47e-22 88.6
Msa0096960 MtrunA17_Chr8g0385441 76.923 52 12 0 101 152 93 144 2.76e-21 87.0
Msa0096960 MtrunA17_Chr2g0283141 35.577 104 55 1 49 152 26 117 3.87e-14 71.2
Msa0096960 MtrunA17_Chr7g0223471 46.774 62 33 0 90 151 47 108 1.68e-13 68.9
Msa0096960 MtrunA17_Chr3g0144021 39.474 76 46 0 90 165 82 157 3.20e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0096960 AT1G46480.1 52.896 259 91 9 1 236 1 251 1.63e-72 221
Msa0096960 AT1G46480.2 59.067 193 60 7 1 182 1 185 3.05e-63 196
Msa0096960 AT3G18010.1 42.857 147 74 2 6 152 2 138 6.13e-30 114
Msa0096960 AT3G11260.1 56.818 88 33 1 87 169 16 103 1.24e-27 104
Msa0096960 AT5G59340.1 61.333 75 29 0 78 152 2 76 5.75e-27 104
Msa0096960 AT2G28610.1 68.182 66 21 0 87 152 5 70 9.84e-27 103
Msa0096960 AT5G05770.1 63.077 65 24 0 90 154 29 93 1.72e-24 94.4
Msa0096960 AT2G17950.1 51.765 85 40 1 89 172 37 121 1.05e-23 96.7
Msa0096960 AT2G01500.1 66.667 63 19 1 84 144 53 115 2.07e-21 90.1
Msa0096960 AT2G33880.2 38.614 101 52 1 49 149 24 114 4.42e-14 71.2
Msa0096960 AT2G33880.1 38.614 101 52 1 49 149 24 114 4.70e-14 70.9
Msa0096960 AT1G20700.2 47.541 61 32 0 90 150 33 93 2.70e-13 65.9
Msa0096960 AT5G45980.1 46.774 62 33 0 90 151 55 116 4.97e-13 67.8
Msa0096960 AT1G20710.1 38.462 91 44 1 97 187 86 164 6.50e-13 65.9
Msa0096960 AT1G20700.3 47.541 61 32 0 90 150 95 155 9.15e-13 65.5
Msa0096960 AT1G20700.1 47.541 61 32 0 90 150 95 155 1.09e-12 65.5
Msa0096960 AT4G35550.1 47.541 61 32 0 90 150 99 159 1.15e-11 63.2

Find 71 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAATTCACACGTGGATTT+TGG 0.121803 1_3:-2700687 Msa0096960:CDS
ACAAATAAGTTTGGTTAATT+TGG 0.133382 1_3:+2699134 None:intergenic
AAGCAAATTAAAGCACATTT+TGG 0.180958 1_3:+2699266 None:intergenic
TCTCGCGTGCTTTGTGGTTT+TGG 0.203041 1_3:+2699852 None:intergenic
AAACTCCTTCATCTGCCTTC+TGG 0.237007 1_3:+2699318 None:intergenic
AACTCCTTCATCTGCCTTCT+GGG 0.285945 1_3:+2699319 None:intergenic
CAACTTTGGACACCCCAAAA+AGG 0.296385 1_3:-2699733 Msa0096960:CDS
ACTAATTCTAGGAGCACTTT+CGG 0.300053 1_3:+2700531 None:intergenic
TGTTCTATTTGTTGTGCATT+TGG 0.303503 1_3:+2699935 None:intergenic
CTAATTCTAGGAGCACTTTC+GGG 0.314910 1_3:+2700532 None:intergenic
ACAAGAACAAATAGGAATAT+TGG 0.328145 1_3:-2699987 Msa0096960:CDS
GAGGAAGAAAGACTAATTCT+AGG 0.342909 1_3:+2700520 None:intergenic
GGATTCCACCTTGTCCCTCC+TGG 0.366142 1_3:+2700013 None:intergenic
ATCTGCCTTCTGGGTGTAAT+GGG 0.376276 1_3:+2699328 None:intergenic
CTTTAGTACTATTACAACTT+TGG 0.384910 1_3:-2699747 Msa0096960:CDS
CATCTGCCTTCTGGGTGTAA+TGG 0.398760 1_3:+2699327 None:intergenic
ATTCCTATTTGTTCTTGTGT+TGG 0.415008 1_3:+2699992 None:intergenic
TTTCTTCTTTCATTTACATT+GGG 0.415761 1_3:-2699233 Msa0096960:three_prime_UTR
AATCCAACACAAGAACAAAT+AGG 0.426122 1_3:-2699995 Msa0096960:CDS
GTAATGTTATTCCATGACTT+TGG 0.426731 1_3:-2699159 Msa0096960:three_prime_UTR
TTCTCTTCCAAGTACTCAAA+TGG 0.427494 1_3:+2699399 None:intergenic
ATTTCTTCTTTCATTTACAT+TGG 0.443688 1_3:-2699234 Msa0096960:three_prime_UTR
TTTGTCTCTCGCGTGCTTTG+TGG 0.448508 1_3:+2699846 None:intergenic
TAGGTTGAAACGCATATTCC+AGG 0.450427 1_3:-2700031 Msa0096960:CDS
TCCACCTTCACCGCAAAGCC+AGG 0.461999 1_3:-2700479 Msa0096960:intron
TTTGTTGATTTCAGCGTAGT+AGG 0.464270 1_3:-2699097 Msa0096960:three_prime_UTR
GAGTTGGCCATTTGAGTACT+TGG 0.464745 1_3:-2699406 Msa0096960:CDS
GGTGTTAGAAGTAACATTAT+CGG 0.465029 1_3:+2700591 None:intergenic
CAGCGTAGTAGGTGTGACTT+TGG 0.468785 1_3:-2699086 Msa0096960:three_prime_UTR
CACAGACACAAGTGTGGTGG+TGG 0.469900 1_3:+2699778 None:intergenic
CAAAGTTGTAATAGTACTAA+AGG 0.472738 1_3:+2699748 None:intergenic
AAGATAAGATTAGTAGAAGT+AGG 0.473815 1_3:+2698971 None:intergenic
AGCAAGTACGGTAAAATTGA+AGG 0.477134 1_3:-2699896 Msa0096960:CDS
CAACTCCTACTCTTCTTCAA+TGG 0.501838 1_3:+2699423 None:intergenic
AGTGTGGTGGTGGGAGTTCG+AGG 0.518160 1_3:+2699788 None:intergenic
CATGAACCTTCCCTCACACT+TGG 0.520457 1_3:-2700661 Msa0096960:CDS
TGAACCTGGCTTTGCGGTGA+AGG 0.521265 1_3:+2700475 None:intergenic
GTTGTAATAGTACTAAAGGT+GGG 0.528602 1_3:+2699752 None:intergenic
TGAGATCAAAAGAAAGAGAA+GGG 0.531578 1_3:+2700569 None:intergenic
TTAATTTGGCTCCAAAGTCA+TGG 0.533407 1_3:+2699148 None:intergenic
AGTATATGAACCTGGCTTTG+CGG 0.544738 1_3:+2700469 None:intergenic
ATAGTCCATTGAAGAAGAGT+AGG 0.545832 1_3:-2699428 Msa0096960:CDS
AAGCAGAAACGTAACAGTCT+TGG 0.547628 1_3:-2699824 Msa0096960:CDS
ATCTTTCTTGTTATCATCAG+AGG 0.556963 1_3:+2700501 None:intergenic
ACAGTTCAACTTAGCAAGTA+CGG 0.562866 1_3:-2699908 Msa0096960:CDS
CGCATATTCCAGGAGGGACA+AGG 0.565252 1_3:-2700021 Msa0096960:CDS
TACACCCAGAAGGCAGATGA+AGG 0.566613 1_3:-2699323 Msa0096960:exon
GTGGGAGTTCGAGGACTATG+AGG 0.567688 1_3:+2699797 None:intergenic
TTGGTCAATGTGCAAAGTAG+AGG 0.568050 1_3:-2699373 Msa0096960:CDS
TTGAGATCAAAAGAAAGAGA+AGG 0.574637 1_3:+2700568 None:intergenic
AGTTGTAATAGTACTAAAGG+TGG 0.581733 1_3:+2699751 None:intergenic
AACGCTTGCAACCAAGTGTG+AGG 0.582297 1_3:+2700650 None:intergenic
GCAAGTACGGTAAAATTGAA+GGG 0.588273 1_3:-2699895 Msa0096960:CDS
CATTGAAGAAGAGTAGGAGT+TGG 0.590517 1_3:-2699422 Msa0096960:CDS
ACCTGGCTTTGCGGTGAAGG+TGG 0.590968 1_3:+2700478 None:intergenic
TGTAATAGTACTAAAGGTGG+GGG 0.603492 1_3:+2699754 None:intergenic
TTGTAATAGTACTAAAGGTG+GGG 0.606911 1_3:+2699753 None:intergenic
TTGGAGATGTTATACAGAGG+TGG 0.611718 1_3:-2699968 Msa0096960:CDS
GAGTTCGAGGACTATGAGGA+AGG 0.633348 1_3:+2699801 None:intergenic
GATTAGTAGAAGTAGGTGCG+TGG 0.641260 1_3:+2698978 None:intergenic
TATTTCTTCATCAGACCGTG+TGG 0.650611 1_3:-2700747 Msa0096960:five_prime_UTR
ACGCTTGCAACCAAGTGTGA+GGG 0.656705 1_3:+2700651 None:intergenic
TTGAAACGCATATTCCAGGA+GGG 0.657126 1_3:-2700027 Msa0096960:CDS
ACAGACACAAGTGTGGTGGT+GGG 0.659851 1_3:+2699779 None:intergenic
GGACACAGACACAAGTGTGG+TGG 0.666759 1_3:+2699775 None:intergenic
GAGATCAAAAGAAAGAGAAG+GGG 0.671823 1_3:+2700570 None:intergenic
TTGCAACCAAGTGTGAGGGA+AGG 0.674259 1_3:+2700655 None:intergenic
GTTGAAACGCATATTCCAGG+AGG 0.708848 1_3:-2700028 Msa0096960:CDS
ATATTGGAGATGTTATACAG+AGG 0.712720 1_3:-2699971 Msa0096960:CDS
AAAGTGCATCAATTCACACG+TGG 0.742454 1_3:-2700694 Msa0096960:CDS
ATATTCCAGGAGGGACAAGG+TGG 0.756182 1_3:-2700018 Msa0096960:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGACTTAATTAAAAAAAAAA+AGG + chr1_3:2699240-2699259 None:intergenic 10.0%
!!! ATAATACTAATAAAGTATTT+TGG - chr1_3:2699397-2699416 Msa0096960:CDS 10.0%
!! AGACTTAATTAAAAAAAAAA+AGG + chr1_3:2699240-2699259 None:intergenic 10.0%
!!! ATAATACTAATAAAGTATTT+TGG - chr1_3:2699397-2699416 Msa0096960:CDS 10.0%
!! AAATCAACAAATGAAAAAAA+AGG + chr1_3:2700556-2700575 None:intergenic 15.0%
!! AAATCAACAAATGAAAAAAA+AGG + chr1_3:2700556-2700575 None:intergenic 15.0%
!!! GTTTAATATAATTTTCCACA+CGG + chr1_3:2698933-2698952 None:intergenic 20.0%
!!! GTGTTATTAGAAGAAATTTT+AGG + chr1_3:2699052-2699071 None:intergenic 20.0%
!! ATTAACTTCATGTAACATAT+AGG - chr1_3:2699354-2699373 Msa0096960:CDS 20.0%
!! TTAACTTCATGTAACATATA+GGG - chr1_3:2699355-2699374 Msa0096960:CDS 20.0%
!! TCTATTATTTGTGTATGTAT+AGG - chr1_3:2699612-2699631 Msa0096960:intron 20.0%
!!! TTTTTGATTAAAAGGAAAGT+AGG - chr1_3:2700076-2700095 Msa0096960:intron 20.0%
!!! TTGTTCATGGTTTTAAATTA+CGG + chr1_3:2700103-2700122 None:intergenic 20.0%
!! AATTAGTATACTATTGTTCA+TGG + chr1_3:2700116-2700135 None:intergenic 20.0%
!! AATACTATAATCTTGTTACT+AGG - chr1_3:2700138-2700157 Msa0096960:intron 20.0%
!! ATTTCTTCTTTCATTTACAT+TGG - chr1_3:2700428-2700447 Msa0096960:intron 20.0%
!! TTTCTTCTTTCATTTACATT+GGG - chr1_3:2700429-2700448 Msa0096960:intron 20.0%
!! ACAAATAAGTTTGGTTAATT+TGG + chr1_3:2700531-2700550 None:intergenic 20.0%
!!! ATACTATACTCTCATATTTT+AGG - chr1_3:2700729-2700748 Msa0096960:five_prime_UTR 20.0%
!!! GTTTAATATAATTTTCCACA+CGG + chr1_3:2698933-2698952 None:intergenic 20.0%
!!! GTGTTATTAGAAGAAATTTT+AGG + chr1_3:2699052-2699071 None:intergenic 20.0%
!! ATTAACTTCATGTAACATAT+AGG - chr1_3:2699354-2699373 Msa0096960:CDS 20.0%
!! TTAACTTCATGTAACATATA+GGG - chr1_3:2699355-2699374 Msa0096960:CDS 20.0%
!! TCTATTATTTGTGTATGTAT+AGG - chr1_3:2699612-2699631 Msa0096960:intron 20.0%
!!! TTTTTGATTAAAAGGAAAGT+AGG - chr1_3:2700076-2700095 Msa0096960:intron 20.0%
!!! TTGTTCATGGTTTTAAATTA+CGG + chr1_3:2700103-2700122 None:intergenic 20.0%
!! AATTAGTATACTATTGTTCA+TGG + chr1_3:2700116-2700135 None:intergenic 20.0%
!! AATACTATAATCTTGTTACT+AGG - chr1_3:2700138-2700157 Msa0096960:intron 20.0%
!! ATTTCTTCTTTCATTTACAT+TGG - chr1_3:2700428-2700447 Msa0096960:intron 20.0%
!! TTTCTTCTTTCATTTACATT+GGG - chr1_3:2700429-2700448 Msa0096960:intron 20.0%
!! ACAAATAAGTTTGGTTAATT+TGG + chr1_3:2700531-2700550 None:intergenic 20.0%
!!! ATACTATACTCTCATATTTT+AGG - chr1_3:2700729-2700748 Msa0096960:five_prime_UTR 20.0%
!!! ACTAATAAAGTATTTTGGTC+CGG - chr1_3:2699402-2699421 Msa0096960:CDS 25.0%
! TACATCTGATAACTGAAATT+CGG + chr1_3:2699442-2699461 None:intergenic 25.0%
!!! TCGTTTAAAAGCTTTTGATT+TGG - chr1_3:2699562-2699581 Msa0096960:intron 25.0%
! ACAAGAACAAATAGGAATAT+TGG - chr1_3:2699675-2699694 Msa0096960:intron 25.0%
! CAAAGTTGTAATAGTACTAA+AGG + chr1_3:2699917-2699936 None:intergenic 25.0%
! CTTTAGTACTATTACAACTT+TGG - chr1_3:2699915-2699934 Msa0096960:CDS 25.0%
!! AATATGAACATACCCTTTTT+GGG + chr1_3:2699945-2699964 None:intergenic 25.0%
! TCAACTTAAGCTATATATTG+TGG - chr1_3:2699975-2699994 Msa0096960:CDS 25.0%
!! CGACAATATAAAGGATTTTA+AGG + chr1_3:2700023-2700042 None:intergenic 25.0%
!!! TCGAATGTGTTACATTTTTT+TGG - chr1_3:2700179-2700198 Msa0096960:intron 25.0%
!! AAGCAAATTAAAGCACATTT+TGG + chr1_3:2700399-2700418 None:intergenic 25.0%
! AAAAAGGTGACAAATAAGTT+TGG + chr1_3:2700540-2700559 None:intergenic 25.0%
! AAGATAAGATTAGTAGAAGT+AGG + chr1_3:2700694-2700713 None:intergenic 25.0%
!!! ACTAATAAAGTATTTTGGTC+CGG - chr1_3:2699402-2699421 Msa0096960:CDS 25.0%
! TACATCTGATAACTGAAATT+CGG + chr1_3:2699442-2699461 None:intergenic 25.0%
!!! TCGTTTAAAAGCTTTTGATT+TGG - chr1_3:2699562-2699581 Msa0096960:intron 25.0%
! ACAAGAACAAATAGGAATAT+TGG - chr1_3:2699675-2699694 Msa0096960:intron 25.0%
! CAAAGTTGTAATAGTACTAA+AGG + chr1_3:2699917-2699936 None:intergenic 25.0%
! CTTTAGTACTATTACAACTT+TGG - chr1_3:2699915-2699934 Msa0096960:CDS 25.0%
!! AATATGAACATACCCTTTTT+GGG + chr1_3:2699945-2699964 None:intergenic 25.0%
! TCAACTTAAGCTATATATTG+TGG - chr1_3:2699975-2699994 Msa0096960:CDS 25.0%
!! CGACAATATAAAGGATTTTA+AGG + chr1_3:2700023-2700042 None:intergenic 25.0%
!!! TCGAATGTGTTACATTTTTT+TGG - chr1_3:2700179-2700198 Msa0096960:intron 25.0%
!! AAGCAAATTAAAGCACATTT+TGG + chr1_3:2700399-2700418 None:intergenic 25.0%
! AAAAAGGTGACAAATAAGTT+TGG + chr1_3:2700540-2700559 None:intergenic 25.0%
! AAGATAAGATTAGTAGAAGT+AGG + chr1_3:2700694-2700713 None:intergenic 25.0%
GGTGTTAGAAGTAACATTAT+CGG + chr1_3:2699074-2699093 None:intergenic 30.0%
TGAGATCAAAAGAAAGAGAA+GGG + chr1_3:2699096-2699115 None:intergenic 30.0%
TTGAGATCAAAAGAAAGAGA+AGG + chr1_3:2699097-2699116 None:intergenic 30.0%
! ATCTTTCTTGTTATCATCAG+AGG + chr1_3:2699164-2699183 None:intergenic 30.0%
!!! CTAATAAAGTATTTTGGTCC+GGG - chr1_3:2699403-2699422 Msa0096960:CDS 30.0%
AATCCAACACAAGAACAAAT+AGG - chr1_3:2699667-2699686 Msa0096960:intron 30.0%
ATTCCTATTTGTTCTTGTGT+TGG + chr1_3:2699673-2699692 None:intergenic 30.0%
ATATTGGAGATGTTATACAG+AGG - chr1_3:2699691-2699710 Msa0096960:intron 30.0%
!! TGTTCTATTTGTTGTGCATT+TGG + chr1_3:2699730-2699749 None:intergenic 30.0%
AAGGGAAAAATGTGTTCTAT+TGG - chr1_3:2699785-2699804 Msa0096960:CDS 30.0%
TTGTAATAGTACTAAAGGTG+GGG + chr1_3:2699912-2699931 None:intergenic 30.0%
GTTGTAATAGTACTAAAGGT+GGG + chr1_3:2699913-2699932 None:intergenic 30.0%
AGTTGTAATAGTACTAAAGG+TGG + chr1_3:2699914-2699933 None:intergenic 30.0%
! ATATGAACATACCCTTTTTG+GGG + chr1_3:2699944-2699963 None:intergenic 30.0%
! GAATATGAACATACCCTTTT+TGG + chr1_3:2699946-2699965 None:intergenic 30.0%
!!! TGTGTTACATTTTTTTGGAC+AGG - chr1_3:2700184-2700203 Msa0096960:intron 30.0%
AGTACTTGGAAGAGAAAAAT+TGG - chr1_3:2700270-2700289 Msa0096960:intron 30.0%
GTAATGTTATTCCATGACTT+TGG - chr1_3:2700503-2700522 Msa0096960:CDS 30.0%
AAGCTTATGTCGTCTAAAAA+AGG + chr1_3:2700613-2700632 None:intergenic 30.0%
!!! TCGAAAGCTTTTAGGTTAAA+AGG - chr1_3:2700638-2700657 Msa0096960:CDS 30.0%
GGTGTTAGAAGTAACATTAT+CGG + chr1_3:2699074-2699093 None:intergenic 30.0%
TGAGATCAAAAGAAAGAGAA+GGG + chr1_3:2699096-2699115 None:intergenic 30.0%
TTGAGATCAAAAGAAAGAGA+AGG + chr1_3:2699097-2699116 None:intergenic 30.0%
! ATCTTTCTTGTTATCATCAG+AGG + chr1_3:2699164-2699183 None:intergenic 30.0%
!!! CTAATAAAGTATTTTGGTCC+GGG - chr1_3:2699403-2699422 Msa0096960:CDS 30.0%
AATCCAACACAAGAACAAAT+AGG - chr1_3:2699667-2699686 Msa0096960:intron 30.0%
ATTCCTATTTGTTCTTGTGT+TGG + chr1_3:2699673-2699692 None:intergenic 30.0%
ATATTGGAGATGTTATACAG+AGG - chr1_3:2699691-2699710 Msa0096960:intron 30.0%
!! TGTTCTATTTGTTGTGCATT+TGG + chr1_3:2699730-2699749 None:intergenic 30.0%
AAGGGAAAAATGTGTTCTAT+TGG - chr1_3:2699785-2699804 Msa0096960:CDS 30.0%
TTGTAATAGTACTAAAGGTG+GGG + chr1_3:2699912-2699931 None:intergenic 30.0%
GTTGTAATAGTACTAAAGGT+GGG + chr1_3:2699913-2699932 None:intergenic 30.0%
AGTTGTAATAGTACTAAAGG+TGG + chr1_3:2699914-2699933 None:intergenic 30.0%
! ATATGAACATACCCTTTTTG+GGG + chr1_3:2699944-2699963 None:intergenic 30.0%
! GAATATGAACATACCCTTTT+TGG + chr1_3:2699946-2699965 None:intergenic 30.0%
!!! TGTGTTACATTTTTTTGGAC+AGG - chr1_3:2700184-2700203 Msa0096960:intron 30.0%
AGTACTTGGAAGAGAAAAAT+TGG - chr1_3:2700270-2700289 Msa0096960:intron 30.0%
GTAATGTTATTCCATGACTT+TGG - chr1_3:2700503-2700522 Msa0096960:CDS 30.0%
AAGCTTATGTCGTCTAAAAA+AGG + chr1_3:2700613-2700632 None:intergenic 30.0%
!!! TCGAAAGCTTTTAGGTTAAA+AGG - chr1_3:2700638-2700657 Msa0096960:CDS 30.0%
! ATCAATTCACACGTGGATTT+TGG - chr1_3:2698975-2698994 Msa0096960:three_prime_UTR 35.0%
! TCAATTCACACGTGGATTTT+GGG - chr1_3:2698976-2698995 Msa0096960:three_prime_UTR 35.0%
!! GAAGAAATTTTAGGTGCAAG+AGG + chr1_3:2699043-2699062 None:intergenic 35.0%
GAGATCAAAAGAAAGAGAAG+GGG + chr1_3:2699095-2699114 None:intergenic 35.0%
!! ACTAATTCTAGGAGCACTTT+CGG + chr1_3:2699134-2699153 None:intergenic 35.0%
GAGGAAGAAAGACTAATTCT+AGG + chr1_3:2699145-2699164 None:intergenic 35.0%
TAGAGCTTAGTATATGAACC+TGG + chr1_3:2699204-2699223 None:intergenic 35.0%
AATTATACCACTCTACTGCA+TGG + chr1_3:2699269-2699288 None:intergenic 35.0%
! TTCGGTTCAACAATTTTTCC+CGG + chr1_3:2699424-2699443 None:intergenic 35.0%
ACAGTTCAACTTAGCAAGTA+CGG - chr1_3:2699754-2699773 Msa0096960:CDS 35.0%
AGCAAGTACGGTAAAATTGA+AGG - chr1_3:2699766-2699785 Msa0096960:CDS 35.0%
GCAAGTACGGTAAAATTGAA+GGG - chr1_3:2699767-2699786 Msa0096960:CDS 35.0%
TGTAATAGTACTAAAGGTGG+GGG + chr1_3:2699911-2699930 None:intergenic 35.0%
! TAGTTGCGTCGACAATATAA+AGG + chr1_3:2700032-2700051 None:intergenic 35.0%
ATAGTCCATTGAAGAAGAGT+AGG - chr1_3:2700234-2700253 Msa0096960:intron 35.0%
TTCTCTTCCAAGTACTCAAA+TGG + chr1_3:2700266-2700285 None:intergenic 35.0%
! TTAATTTGGCTCCAAAGTCA+TGG + chr1_3:2700517-2700536 None:intergenic 35.0%
!! TTTGTTGATTTCAGCGTAGT+AGG - chr1_3:2700565-2700584 Msa0096960:CDS 35.0%
! CTTGTGAATCGAAAGCTTTT+AGG - chr1_3:2700630-2700649 Msa0096960:CDS 35.0%
! ATCAATTCACACGTGGATTT+TGG - chr1_3:2698975-2698994 Msa0096960:three_prime_UTR 35.0%
! TCAATTCACACGTGGATTTT+GGG - chr1_3:2698976-2698995 Msa0096960:three_prime_UTR 35.0%
!! GAAGAAATTTTAGGTGCAAG+AGG + chr1_3:2699043-2699062 None:intergenic 35.0%
GAGATCAAAAGAAAGAGAAG+GGG + chr1_3:2699095-2699114 None:intergenic 35.0%
!! ACTAATTCTAGGAGCACTTT+CGG + chr1_3:2699134-2699153 None:intergenic 35.0%
GAGGAAGAAAGACTAATTCT+AGG + chr1_3:2699145-2699164 None:intergenic 35.0%
TAGAGCTTAGTATATGAACC+TGG + chr1_3:2699204-2699223 None:intergenic 35.0%
AATTATACCACTCTACTGCA+TGG + chr1_3:2699269-2699288 None:intergenic 35.0%
! TTCGGTTCAACAATTTTTCC+CGG + chr1_3:2699424-2699443 None:intergenic 35.0%
ACAGTTCAACTTAGCAAGTA+CGG - chr1_3:2699754-2699773 Msa0096960:CDS 35.0%
AGCAAGTACGGTAAAATTGA+AGG - chr1_3:2699766-2699785 Msa0096960:CDS 35.0%
GCAAGTACGGTAAAATTGAA+GGG - chr1_3:2699767-2699786 Msa0096960:CDS 35.0%
TGTAATAGTACTAAAGGTGG+GGG + chr1_3:2699911-2699930 None:intergenic 35.0%
! TAGTTGCGTCGACAATATAA+AGG + chr1_3:2700032-2700051 None:intergenic 35.0%
ATAGTCCATTGAAGAAGAGT+AGG - chr1_3:2700234-2700253 Msa0096960:intron 35.0%
TTCTCTTCCAAGTACTCAAA+TGG + chr1_3:2700266-2700285 None:intergenic 35.0%
! TTAATTTGGCTCCAAAGTCA+TGG + chr1_3:2700517-2700536 None:intergenic 35.0%
!! TTTGTTGATTTCAGCGTAGT+AGG - chr1_3:2700565-2700584 Msa0096960:CDS 35.0%
! CTTGTGAATCGAAAGCTTTT+AGG - chr1_3:2700630-2700649 Msa0096960:CDS 35.0%
TATTTCTTCATCAGACCGTG+TGG - chr1_3:2698915-2698934 Msa0096960:three_prime_UTR 40.0%
AAAGTGCATCAATTCACACG+TGG - chr1_3:2698968-2698987 Msa0096960:three_prime_UTR 40.0%
!! CTAATTCTAGGAGCACTTTC+GGG + chr1_3:2699133-2699152 None:intergenic 40.0%
!! AGTATATGAACCTGGCTTTG+CGG + chr1_3:2699196-2699215 None:intergenic 40.0%
CGAGTCTCATATGGAAAAAG+AGG + chr1_3:2699504-2699523 None:intergenic 40.0%
AGTAACTCTCGAGTCTCATA+TGG + chr1_3:2699513-2699532 None:intergenic 40.0%
! TAGGTTGAAACGCATATTCC+AGG - chr1_3:2699631-2699650 Msa0096960:intron 40.0%
TTGAAACGCATATTCCAGGA+GGG - chr1_3:2699635-2699654 Msa0096960:intron 40.0%
TTGGAGATGTTATACAGAGG+TGG - chr1_3:2699694-2699713 Msa0096960:intron 40.0%
AAGCAGAAACGTAACAGTCT+TGG - chr1_3:2699838-2699857 Msa0096960:CDS 40.0%
AACTTTGGACACCCCAAAAA+GGG - chr1_3:2699930-2699949 Msa0096960:CDS 40.0%
! TTTTGGACAGGTGCAAGTAA+TGG - chr1_3:2700196-2700215 Msa0096960:intron 40.0%
CAACTCCTACTCTTCTTCAA+TGG + chr1_3:2700242-2700261 None:intergenic 40.0%
CATTGAAGAAGAGTAGGAGT+TGG - chr1_3:2700240-2700259 Msa0096960:intron 40.0%
TTGGTCAATGTGCAAAGTAG+AGG - chr1_3:2700289-2700308 Msa0096960:intron 40.0%
TATTTCTTCATCAGACCGTG+TGG - chr1_3:2698915-2698934 Msa0096960:three_prime_UTR 40.0%
AAAGTGCATCAATTCACACG+TGG - chr1_3:2698968-2698987 Msa0096960:three_prime_UTR 40.0%
!! CTAATTCTAGGAGCACTTTC+GGG + chr1_3:2699133-2699152 None:intergenic 40.0%
!! AGTATATGAACCTGGCTTTG+CGG + chr1_3:2699196-2699215 None:intergenic 40.0%
CGAGTCTCATATGGAAAAAG+AGG + chr1_3:2699504-2699523 None:intergenic 40.0%
AGTAACTCTCGAGTCTCATA+TGG + chr1_3:2699513-2699532 None:intergenic 40.0%
! TAGGTTGAAACGCATATTCC+AGG - chr1_3:2699631-2699650 Msa0096960:intron 40.0%
TTGAAACGCATATTCCAGGA+GGG - chr1_3:2699635-2699654 Msa0096960:intron 40.0%
TTGGAGATGTTATACAGAGG+TGG - chr1_3:2699694-2699713 Msa0096960:intron 40.0%
AAGCAGAAACGTAACAGTCT+TGG - chr1_3:2699838-2699857 Msa0096960:CDS 40.0%
AACTTTGGACACCCCAAAAA+GGG - chr1_3:2699930-2699949 Msa0096960:CDS 40.0%
! TTTTGGACAGGTGCAAGTAA+TGG - chr1_3:2700196-2700215 Msa0096960:intron 40.0%
CAACTCCTACTCTTCTTCAA+TGG + chr1_3:2700242-2700261 None:intergenic 40.0%
CATTGAAGAAGAGTAGGAGT+TGG - chr1_3:2700240-2700259 Msa0096960:intron 40.0%
TTGGTCAATGTGCAAAGTAG+AGG - chr1_3:2700289-2700308 Msa0096960:intron 40.0%
ATACCACTCTACTGCATGGA+TGG + chr1_3:2699265-2699284 None:intergenic 45.0%
GTTGAAACGCATATTCCAGG+AGG - chr1_3:2699634-2699653 Msa0096960:intron 45.0%
CAACTTTGGACACCCCAAAA+AGG - chr1_3:2699929-2699948 Msa0096960:CDS 45.0%
TGTCGACGCAACTATAGTCT+TGG - chr1_3:2700038-2700057 Msa0096960:intron 45.0%
GAGTTGGCCATTTGAGTACT+TGG - chr1_3:2700256-2700275 Msa0096960:intron 45.0%
! CTTTTCCCATTACACCCAGA+AGG - chr1_3:2700329-2700348 Msa0096960:intron 45.0%
ATCTGCCTTCTGGGTGTAAT+GGG + chr1_3:2700337-2700356 None:intergenic 45.0%
AACTCCTTCATCTGCCTTCT+GGG + chr1_3:2700346-2700365 None:intergenic 45.0%
AAACTCCTTCATCTGCCTTC+TGG + chr1_3:2700347-2700366 None:intergenic 45.0%
GATTAGTAGAAGTAGGTGCG+TGG + chr1_3:2700687-2700706 None:intergenic 45.0%
ATACCACTCTACTGCATGGA+TGG + chr1_3:2699265-2699284 None:intergenic 45.0%
GTTGAAACGCATATTCCAGG+AGG - chr1_3:2699634-2699653 Msa0096960:intron 45.0%
CAACTTTGGACACCCCAAAA+AGG - chr1_3:2699929-2699948 Msa0096960:CDS 45.0%
TGTCGACGCAACTATAGTCT+TGG - chr1_3:2700038-2700057 Msa0096960:intron 45.0%
GAGTTGGCCATTTGAGTACT+TGG - chr1_3:2700256-2700275 Msa0096960:intron 45.0%
! CTTTTCCCATTACACCCAGA+AGG - chr1_3:2700329-2700348 Msa0096960:intron 45.0%
ATCTGCCTTCTGGGTGTAAT+GGG + chr1_3:2700337-2700356 None:intergenic 45.0%
AACTCCTTCATCTGCCTTCT+GGG + chr1_3:2700346-2700365 None:intergenic 45.0%
AAACTCCTTCATCTGCCTTC+TGG + chr1_3:2700347-2700366 None:intergenic 45.0%
GATTAGTAGAAGTAGGTGCG+TGG + chr1_3:2700687-2700706 None:intergenic 45.0%
!! AATCAAAAAAAAAAAAATTA+CGG + chr1_3:2700065-2700084 None:intergenic 5.0%
!!! TTTTTTTTTTTTTGATTAAA+AGG - chr1_3:2700068-2700087 Msa0096960:intron 5.0%
!! AATCAAAAAAAAAAAAATTA+CGG + chr1_3:2700065-2700084 None:intergenic 5.0%
!!! TTTTTTTTTTTTTGATTAAA+AGG - chr1_3:2700068-2700087 Msa0096960:intron 5.0%
CATGAACCTTCCCTCACACT+TGG - chr1_3:2699001-2699020 Msa0096960:three_prime_UTR 50.0%
TTGCAACCAAGTGTGAGGGA+AGG + chr1_3:2699010-2699029 None:intergenic 50.0%
ACGCTTGCAACCAAGTGTGA+GGG + chr1_3:2699014-2699033 None:intergenic 50.0%
AACGCTTGCAACCAAGTGTG+AGG + chr1_3:2699015-2699034 None:intergenic 50.0%
TGTCCATCCATGCAGTAGAG+TGG - chr1_3:2699259-2699278 Msa0096960:three_prime_UTR 50.0%
ATATTCCAGGAGGGACAAGG+TGG - chr1_3:2699644-2699663 Msa0096960:intron 50.0%
! TCTCGCGTGCTTTGTGGTTT+TGG + chr1_3:2699813-2699832 None:intergenic 50.0%
TTTGTCTCTCGCGTGCTTTG+TGG + chr1_3:2699819-2699838 None:intergenic 50.0%
GAGTTCGAGGACTATGAGGA+AGG + chr1_3:2699864-2699883 None:intergenic 50.0%
ACAGACACAAGTGTGGTGGT+GGG + chr1_3:2699886-2699905 None:intergenic 50.0%
AGGTCTCTGCAACGACGTTT+CGG + chr1_3:2700003-2700022 None:intergenic 50.0%
CATCTGCCTTCTGGGTGTAA+TGG + chr1_3:2700338-2700357 None:intergenic 50.0%
TACACCCAGAAGGCAGATGA+AGG - chr1_3:2700339-2700358 Msa0096960:intron 50.0%
CAGCGTAGTAGGTGTGACTT+TGG - chr1_3:2700576-2700595 Msa0096960:CDS 50.0%
CATGAACCTTCCCTCACACT+TGG - chr1_3:2699001-2699020 Msa0096960:three_prime_UTR 50.0%
TTGCAACCAAGTGTGAGGGA+AGG + chr1_3:2699010-2699029 None:intergenic 50.0%
ACGCTTGCAACCAAGTGTGA+GGG + chr1_3:2699014-2699033 None:intergenic 50.0%
AACGCTTGCAACCAAGTGTG+AGG + chr1_3:2699015-2699034 None:intergenic 50.0%
TGTCCATCCATGCAGTAGAG+TGG - chr1_3:2699259-2699278 Msa0096960:three_prime_UTR 50.0%
ATATTCCAGGAGGGACAAGG+TGG - chr1_3:2699644-2699663 Msa0096960:intron 50.0%
! TCTCGCGTGCTTTGTGGTTT+TGG + chr1_3:2699813-2699832 None:intergenic 50.0%
TTTGTCTCTCGCGTGCTTTG+TGG + chr1_3:2699819-2699838 None:intergenic 50.0%
GAGTTCGAGGACTATGAGGA+AGG + chr1_3:2699864-2699883 None:intergenic 50.0%
ACAGACACAAGTGTGGTGGT+GGG + chr1_3:2699886-2699905 None:intergenic 50.0%
AGGTCTCTGCAACGACGTTT+CGG + chr1_3:2700003-2700022 None:intergenic 50.0%
CATCTGCCTTCTGGGTGTAA+TGG + chr1_3:2700338-2700357 None:intergenic 50.0%
TACACCCAGAAGGCAGATGA+AGG - chr1_3:2700339-2700358 Msa0096960:intron 50.0%
CAGCGTAGTAGGTGTGACTT+TGG - chr1_3:2700576-2700595 Msa0096960:CDS 50.0%
!! TGAACCTGGCTTTGCGGTGA+AGG + chr1_3:2699190-2699209 None:intergenic 55.0%
CGCATATTCCAGGAGGGACA+AGG - chr1_3:2699641-2699660 Msa0096960:intron 55.0%
GTGGGAGTTCGAGGACTATG+AGG + chr1_3:2699868-2699887 None:intergenic 55.0%
CACAGACACAAGTGTGGTGG+TGG + chr1_3:2699887-2699906 None:intergenic 55.0%
GGACACAGACACAAGTGTGG+TGG + chr1_3:2699890-2699909 None:intergenic 55.0%
!! TGAACCTGGCTTTGCGGTGA+AGG + chr1_3:2699190-2699209 None:intergenic 55.0%
CGCATATTCCAGGAGGGACA+AGG - chr1_3:2699641-2699660 Msa0096960:intron 55.0%
GTGGGAGTTCGAGGACTATG+AGG + chr1_3:2699868-2699887 None:intergenic 55.0%
CACAGACACAAGTGTGGTGG+TGG + chr1_3:2699887-2699906 None:intergenic 55.0%
GGACACAGACACAAGTGTGG+TGG + chr1_3:2699890-2699909 None:intergenic 55.0%
TCCACCTTCACCGCAAAGCC+AGG - chr1_3:2699183-2699202 Msa0096960:three_prime_UTR 60.0%
!! ACCTGGCTTTGCGGTGAAGG+TGG + chr1_3:2699187-2699206 None:intergenic 60.0%
GGATTCCACCTTGTCCCTCC+TGG + chr1_3:2699652-2699671 None:intergenic 60.0%
!! AGTGTGGTGGTGGGAGTTCG+AGG + chr1_3:2699877-2699896 None:intergenic 60.0%
GGGGGACACAGACACAAGTG+TGG + chr1_3:2699893-2699912 None:intergenic 60.0%
TCCACCTTCACCGCAAAGCC+AGG - chr1_3:2699183-2699202 Msa0096960:three_prime_UTR 60.0%
!! ACCTGGCTTTGCGGTGAAGG+TGG + chr1_3:2699187-2699206 None:intergenic 60.0%
GGATTCCACCTTGTCCCTCC+TGG + chr1_3:2699652-2699671 None:intergenic 60.0%
!! AGTGTGGTGGTGGGAGTTCG+AGG + chr1_3:2699877-2699896 None:intergenic 60.0%
GGGGGACACAGACACAAGTG+TGG + chr1_3:2699893-2699912 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 2698915 2700769 2698915 ID=Msa0096960;Name=Msa0096960
chr1_3 mRNA 2698915 2700769 2698915 ID=Msa0096960-mRNA-1;Parent=Msa0096960;Name=Msa0096960-mRNA-1;_AED=0.05;_eAED=0.05;_QI=50|1|1|1|1|1|3|411|236
chr1_3 exon 2698915 2699478 2698915 ID=Msa0096960-mRNA-1:exon:770;Parent=Msa0096960-mRNA-1
chr1_3 exon 2699733 2700050 2699733 ID=Msa0096960-mRNA-1:exon:769;Parent=Msa0096960-mRNA-1
chr1_3 exon 2700480 2700769 2700480 ID=Msa0096960-mRNA-1:exon:768;Parent=Msa0096960-mRNA-1
chr1_3 five_prime_UTR 2700720 2700769 2700720 ID=Msa0096960-mRNA-1:five_prime_utr;Parent=Msa0096960-mRNA-1
chr1_3 CDS 2700480 2700719 2700480 ID=Msa0096960-mRNA-1:cds;Parent=Msa0096960-mRNA-1
chr1_3 CDS 2699733 2700050 2699733 ID=Msa0096960-mRNA-1:cds;Parent=Msa0096960-mRNA-1
chr1_3 CDS 2699326 2699478 2699326 ID=Msa0096960-mRNA-1:cds;Parent=Msa0096960-mRNA-1
chr1_3 three_prime_UTR 2698915 2699325 2698915 ID=Msa0096960-mRNA-1:three_prime_utr;Parent=Msa0096960-mRNA-1
Gene Sequence

>Msa0096960

ATGAAAGTGCATCAATTCACACGTGGATTTTGGGAGCATGAACCTTCCCTCACACTTGGTTGCAAGCGTTTACGTCCTCTTGCACCTAAAATTTCTTCTAATAACACCGATAATGTTACTTCTAACACCCCTTCTCTTTCTTTTGATCTCAAGAGCTTCATTAGACCCGAAAGTGCTCCTAGAATTAGTCTTTCTTCCTCTGATGATAACAAGAAAGATCCACCTTCACCGCAAAGCCAGGTTGAAACGCATATTCCAGGAGGGACAAGGTGGAATCCAACACAAGAACAAATAGGAATATTGGAGATGTTATACAGAGGTGGAATGAGAACTCCAAATGCACAACAAATAGAACAGATCACAGTTCAACTTAGCAAGTACGGTAAAATTGAAGGGAAAAATGTGTTCTATTGGTTCCAAAACCACAAAGCACGCGAGAGACAAAAGCAGAAACGTAACAGTCTTGGCCTTCCTCATAGTCCTCGAACTCCCACCACCACACTTGTGTCTGTGTCCCCCACCTTTAGTACTATTACAACTTTGGACACCCCAAAAAGGGTGCAAGTAATGGAAAGAGATCAAGAAGATAGTCCATTGAAGAAGAGTAGGAGTTGGCCATTTGAGTACTTGGAAGAGAAAAATTGGTCAATGTGCAAAGTAGAGGAACATAAAACTCTAGAGCTTTTCCCATTACACCCAGAAGGCAGATGA
Protein sequence

>Msa0096960

MKVHQFTRGFWEHEPSLTLGCKRLRPLAPKISSNNTDNVTSNTPSLSFDLKSFIRPESAPRISLSSSDDNKKDPPSPQSQVETHIPGGTRWNPTQEQIGILEMLYRGGMRTPNAQQIEQITVQLSKYGKIEGKNVFYWFQNHKARERQKQKRNSLGLPHSPRTPTTTLVSVSPTFSTITTLDTPKRVQVMERDQEDSPLKKSRSWPFEYLEEKNWSMCKVEEHKTLELFPLHPEGR*