Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0004930 | Msa0191040 | 0.801167 | 1.023038e-48 | -8.615850e-47 |
| Msa0004930 | Msa0233340 | 0.801369 | 9.303556e-49 | -8.615850e-47 |
| Msa0004930 | Msa0236440 | 0.803101 | 4.093978e-49 | -8.615850e-47 |
| Msa0004930 | Msa0311690 | 0.803298 | 3.726002e-49 | -8.615850e-47 |
| Msa0004930 | Msa0426330 | 0.808720 | 2.695552e-50 | -8.615850e-47 |
| Msa0004930 | Msa0631120 | 0.803221 | 3.866681e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0004930 | MtrunA17_Chr7g0233401 | 53.947 | 76 | 35 | 0 | 28 | 103 | 1 | 76 | 1.02e-21 | 83.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACACAACAAATCTAGCAAAT+TGG | 0.306026 | 1_1:-7372057 | Msa0004930:CDS |
| CATATGGTTTGGGATATAGA+TGG | 0.325146 | 1_1:-7372151 | Msa0004930:CDS |
| TAAACAGATTCATATGGTTT+GGG | 0.337353 | 1_1:-7372161 | Msa0004930:CDS |
| GTAAACAGATTCATATGGTT+TGG | 0.339415 | 1_1:-7372162 | Msa0004930:CDS |
| TGAAGATATGGGTGATAATC+AGG | 0.355872 | 1_1:-7371984 | Msa0004930:CDS |
| GTCTGCGCAAGATGAAGATA+TGG | 0.373665 | 1_1:-7371996 | Msa0004930:CDS |
| TCATCTTGCATTCCTCCTAT+AGG | 0.406756 | 1_1:+7371950 | Msa0004920:intron |
| GTGTCAGTTCTCAATGGATT+TGG | 0.416718 | 1_1:+7372076 | Msa0004920:intron |
| TTTGTTGTGTCAGTTCTCAA+TGG | 0.456390 | 1_1:+7372070 | Msa0004920:intron |
| TTATCTTGATTTAGATCCAC+AGG | 0.480924 | 1_1:-7372032 | Msa0004930:CDS |
| CAGGAGGAACAACAACCTAT+AGG | 0.482504 | 1_1:-7371965 | Msa0004930:CDS |
| ATATGGTTTGGGATATAGAT+GGG | 0.495254 | 1_1:-7372150 | Msa0004930:CDS |
| TCTGCGCAAGATGAAGATAT+GGG | 0.561786 | 1_1:-7371995 | Msa0004930:CDS |
| CCGAAGAAAATCACATTGAG+AGG | 0.609653 | 1_1:-7372210 | Msa0004930:CDS |
| GACTGCTCTTGAAATACCTG+TGG | 0.614927 | 1_1:+7372016 | Msa0004920:intron |
| GAGGAACAACAACCTATAGG+AGG | 0.638621 | 1_1:-7371962 | Msa0004930:CDS |
| AGATATGGGTGATAATCAGG+AGG | 0.694136 | 1_1:-7371981 | Msa0004930:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTACAAAAATCAAGATCAA+TGG | + | chr1_1:7371992-7372011 | Msa0004920:intron | 20.0% |
| !!! | TTTTTGTAAACAGATTCATA+TGG | - | chr1_1:7372002-7372021 | Msa0004930:CDS | 20.0% |
| ! | TAAACAGATTCATATGGTTT+GGG | - | chr1_1:7372008-7372027 | Msa0004930:CDS | 25.0% |
| GTAAACAGATTCATATGGTT+TGG | - | chr1_1:7372007-7372026 | Msa0004930:CDS | 30.0% | |
| ! | ATATGGTTTGGGATATAGAT+GGG | - | chr1_1:7372019-7372038 | Msa0004930:CDS | 30.0% |
| ACACAACAAATCTAGCAAAT+TGG | - | chr1_1:7372112-7372131 | Msa0004930:CDS | 30.0% | |
| TTATCTTGATTTAGATCCAC+AGG | - | chr1_1:7372137-7372156 | Msa0004930:CDS | 30.0% | |
| ! | CCTCTCAATGTGATTTTCTT+CGG | + | chr1_1:7371962-7371981 | Msa0004920:intron | 35.0% |
| ! | CATATGGTTTGGGATATAGA+TGG | - | chr1_1:7372018-7372037 | Msa0004930:CDS | 35.0% |
| ! | TTTGTTGTGTCAGTTCTCAA+TGG | + | chr1_1:7372102-7372121 | Msa0004920:intron | 35.0% |
| TGAAGATATGGGTGATAATC+AGG | - | chr1_1:7372185-7372204 | Msa0004930:CDS | 35.0% | |
| CCGAAGAAAATCACATTGAG+AGG | - | chr1_1:7371959-7371978 | Msa0004930:CDS | 40.0% | |
| ! | GTGTCAGTTCTCAATGGATT+TGG | + | chr1_1:7372096-7372115 | Msa0004920:intron | 40.0% |
| TCTGCGCAAGATGAAGATAT+GGG | - | chr1_1:7372174-7372193 | Msa0004930:CDS | 40.0% | |
| AGATATGGGTGATAATCAGG+AGG | - | chr1_1:7372188-7372207 | Msa0004930:CDS | 40.0% | |
| TCATCTTGCATTCCTCCTAT+AGG | + | chr1_1:7372222-7372241 | Msa0004920:intron | 40.0% | |
| GACTGCTCTTGAAATACCTG+TGG | + | chr1_1:7372156-7372175 | Msa0004920:intron | 45.0% | |
| GTCTGCGCAAGATGAAGATA+TGG | - | chr1_1:7372173-7372192 | Msa0004930:CDS | 45.0% | |
| CAGGAGGAACAACAACCTAT+AGG | - | chr1_1:7372204-7372223 | Msa0004930:CDS | 45.0% | |
| GAGGAACAACAACCTATAGG+AGG | - | chr1_1:7372207-7372226 | Msa0004930:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_1 | gene | 7371940 | 7372251 | 7371940 | ID=Msa0004930;Name=Msa0004930 |
| chr1_1 | mRNA | 7371940 | 7372251 | 7371940 | ID=Msa0004930-mRNA-1;Parent=Msa0004930;Name=Msa0004930-mRNA-1;_AED=0.34;_eAED=0.34;_QI=0|-1|0|1|-1|1|1|0|103 |
| chr1_1 | exon | 7371940 | 7372251 | 7371940 | ID=Msa0004930-mRNA-1:exon:2587;Parent=Msa0004930-mRNA-1 |
| chr1_1 | CDS | 7371940 | 7372251 | 7371940 | ID=Msa0004930-mRNA-1:cds;Parent=Msa0004930-mRNA-1 |
| Gene Sequence |
| Protein sequence |
