AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0311690


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0311690 MtrunA17_Chr2g0280881 99.153 118 1 0 1 118 1 118 2.47e-82 236
Msa0311690 MtrunA17_Chr2g0323211 64.151 106 38 0 9 114 5 110 8.65e-44 139
Msa0311690 MtrunA17_Chr2g0318861 59.649 114 42 1 1 114 1 110 1.71e-40 130
Msa0311690 MtrunA17_Chr8g0361421 59.091 110 45 0 5 114 2 111 2.75e-39 127
Msa0311690 MtrunA17_Chr2g0318791 61.905 105 33 2 14 118 9 106 2.51e-38 125
Msa0311690 MtrunA17_Chr4g0023231 59.406 101 41 0 14 114 6 106 2.52e-38 125
Msa0311690 MtrunA17_Chr2g0318761 65.957 94 31 1 14 107 5 97 4.30e-38 124
Msa0311690 MtrunA17_Chr2g0318801 63.830 94 33 1 14 107 9 101 7.75e-36 119
Msa0311690 MtrunA17_Chr8g0346481 56.436 101 44 0 14 114 19 119 1.21e-35 118
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0311690 AT4G27280.1 58.824 102 41 1 14 114 19 120 8.55e-38 124
Msa0311690 AT5G54490.1 55.882 102 44 1 14 114 19 120 1.15e-35 119
Msa0311690 AT2G46600.1 52.475 101 48 0 14 114 22 122 4.54e-34 115

Find 26 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTTCAATGATGGAGAAAT+TGG 0.086263 2_4:+6593687 Msa0311690:CDS
AACCGATCCACCATTCTTCT+TGG 0.251298 2_4:-6593647 None:intergenic
GAGTGACTCTAAGCAACTTT+TGG 0.324838 2_4:+6593942 Msa0311690:CDS
CCCAATTTCTCCATCATTGA+AGG 0.334569 2_4:-6593688 None:intergenic
CCTTCAATGATGGAGAAATT+GGG 0.434103 2_4:+6593688 Msa0311690:CDS
AAACTCTGCATTGCTTGGGT+TGG 0.475429 2_4:+6593801 Msa0311690:CDS
CTAAGAGAAGGTGATATTGA+TGG 0.479725 2_4:+6593856 Msa0311690:CDS
GGGAAGTGAAGGGTTTATGA+AGG 0.496833 2_4:+6593708 Msa0311690:CDS
TCTGCATTGCTTGGGTTGGA+AGG 0.497566 2_4:+6593805 Msa0311690:CDS
GGACTACTTCCCTTCAATGA+TGG 0.499786 2_4:+6593678 Msa0311690:CDS
TTTATGAAGGAGTTGGCAAA+TGG 0.508232 2_4:+6593721 Msa0311690:CDS
TAAGAGAAGGTGATATTGAT+GGG 0.513310 2_4:+6593857 Msa0311690:CDS
GAAGGTGATATTGATGGGGA+TGG 0.523087 2_4:+6593862 Msa0311690:CDS
TGAAGGGTTTATGAAGGAGT+TGG 0.524979 2_4:+6593714 Msa0311690:CDS
CCAATTTCTCCATCATTGAA+GGG 0.539401 2_4:-6593687 None:intergenic
ATGGAGAAATTGGGAAGTGA+AGG 0.543176 2_4:+6593697 Msa0311690:CDS
GCGTCGAGTTCCAAGAAGAA+TGG 0.562292 2_4:+6593637 Msa0311690:CDS
GGATGGAGCTCTTAATGAGA+TGG 0.581359 2_4:+6593879 Msa0311690:CDS
TGGAGAAATTGGGAAGTGAA+GGG 0.582244 2_4:+6593698 Msa0311690:CDS
TTAGAGTCACTCATCAAAGC+AGG 0.587443 2_4:-6593931 None:intergenic
TTCCAAGAAGAATGGTGGAT+CGG 0.596507 2_4:+6593645 Msa0311690:CDS
CAGGACTCAACCTAAACATG+AGG 0.616612 2_4:-6593912 None:intergenic
ATTACTTGCATGCTAAGAGA+AGG 0.617118 2_4:+6593844 Msa0311690:CDS
TCGAGTTCCAAGAAGAATGG+TGG 0.624651 2_4:+6593640 Msa0311690:CDS
AAGAGAAGGTGATATTGATG+GGG 0.671155 2_4:+6593858 Msa0311690:CDS
AGGACTCAACCTAAACATGA+GGG 0.684724 2_4:-6593911 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATGGTTTTAATGTGTTGA+TGG + chr2_4:6593738-6593757 Msa0311690:CDS 25.0%
TAAGAGAAGGTGATATTGAT+GGG + chr2_4:6593857-6593876 Msa0311690:CDS 30.0%
CCAATTTCTCCATCATTGAA+GGG - chr2_4:6593690-6593709 None:intergenic 35.0%
! CCTTCAATGATGGAGAAATT+GGG + chr2_4:6593688-6593707 Msa0311690:CDS 35.0%
TTTATGAAGGAGTTGGCAAA+TGG + chr2_4:6593721-6593740 Msa0311690:CDS 35.0%
AAGAAAAACTCTGCATTGCT+TGG + chr2_4:6593796-6593815 Msa0311690:CDS 35.0%
AGAAAAACTCTGCATTGCTT+GGG + chr2_4:6593797-6593816 Msa0311690:CDS 35.0%
ATTACTTGCATGCTAAGAGA+AGG + chr2_4:6593844-6593863 Msa0311690:CDS 35.0%
CTAAGAGAAGGTGATATTGA+TGG + chr2_4:6593856-6593875 Msa0311690:CDS 35.0%
AAGAGAAGGTGATATTGATG+GGG + chr2_4:6593858-6593877 Msa0311690:CDS 35.0%
!! TTGGAAGAAGCTATTTTCAC+TGG + chr2_4:6593961-6593980 Msa0311690:CDS 35.0%
!! TTCCAAGAAGAATGGTGGAT+CGG + chr2_4:6593645-6593664 Msa0311690:CDS 40.0%
! CCCTTCAATGATGGAGAAAT+TGG + chr2_4:6593687-6593706 Msa0311690:CDS 40.0%
CCCAATTTCTCCATCATTGA+AGG - chr2_4:6593691-6593710 None:intergenic 40.0%
ATGGAGAAATTGGGAAGTGA+AGG + chr2_4:6593697-6593716 Msa0311690:CDS 40.0%
TGGAGAAATTGGGAAGTGAA+GGG + chr2_4:6593698-6593717 Msa0311690:CDS 40.0%
TGAAGGGTTTATGAAGGAGT+TGG + chr2_4:6593714-6593733 Msa0311690:CDS 40.0%
! AGTTTTGCACCCTCATGTTT+AGG + chr2_4:6593902-6593921 Msa0311690:CDS 40.0%
AGGACTCAACCTAAACATGA+GGG - chr2_4:6593914-6593933 None:intergenic 40.0%
TTAGAGTCACTCATCAAAGC+AGG - chr2_4:6593934-6593953 None:intergenic 40.0%
! GAGTGACTCTAAGCAACTTT+TGG + chr2_4:6593942-6593961 Msa0311690:CDS 40.0%
TCGAGTTCCAAGAAGAATGG+TGG + chr2_4:6593640-6593659 Msa0311690:CDS 45.0%
AACCGATCCACCATTCTTCT+TGG - chr2_4:6593650-6593669 None:intergenic 45.0%
!!! TGGTGGATCGGTTGTTTTTG+AGG + chr2_4:6593657-6593676 Msa0311690:CDS 45.0%
GGACTACTTCCCTTCAATGA+TGG + chr2_4:6593678-6593697 Msa0311690:CDS 45.0%
GGGAAGTGAAGGGTTTATGA+AGG + chr2_4:6593708-6593727 Msa0311690:CDS 45.0%
AAACTCTGCATTGCTTGGGT+TGG + chr2_4:6593801-6593820 Msa0311690:CDS 45.0%
GAAGGTGATATTGATGGGGA+TGG + chr2_4:6593862-6593881 Msa0311690:CDS 45.0%
GGATGGAGCTCTTAATGAGA+TGG + chr2_4:6593879-6593898 Msa0311690:CDS 45.0%
CAGGACTCAACCTAAACATG+AGG - chr2_4:6593915-6593934 None:intergenic 45.0%
GCGTCGAGTTCCAAGAAGAA+TGG + chr2_4:6593637-6593656 Msa0311690:CDS 50.0%
!! TCTGCATTGCTTGGGTTGGA+AGG + chr2_4:6593805-6593824 Msa0311690:CDS 50.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 6593634 6593990 6593634 ID=Msa0311690;Name=Msa0311690
chr2_4 mRNA 6593634 6593990 6593634 ID=Msa0311690-mRNA-1;Parent=Msa0311690;Name=Msa0311690-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|118
chr2_4 exon 6593634 6593990 6593634 ID=Msa0311690-mRNA-1:exon:2541;Parent=Msa0311690-mRNA-1
chr2_4 CDS 6593634 6593990 6593634 ID=Msa0311690-mRNA-1:cds;Parent=Msa0311690-mRNA-1
Gene Sequence

>Msa0311690

ATGGCGTCGAGTTCCAAGAAGAATGGTGGATCGGTTGTTTTTGAGGACTACTTCCCTTCAATGATGGAGAAATTGGGAAGTGAAGGGTTTATGAAGGAGTTGGCAAATGGTTTTAATGTGTTGATGGACAGAGAGAAGAAAGTGATAACATTTGAGAGCTTGAAGAAAAACTCTGCATTGCTTGGGTTGGAAGGAATGAACGATGATGAGATTACTTGCATGCTAAGAGAAGGTGATATTGATGGGGATGGAGCTCTTAATGAGATGGAGTTTTGCACCCTCATGTTTAGGTTGAGTCCTGCTTTGATGAGTGACTCTAAGCAACTTTTGGAAGAAGCTATTTTCACTGGCCATTAA

Protein sequence

>Msa0311690

MASSSKKNGGSVVFEDYFPSMMEKLGSEGFMKELANGFNVLMDREKKVITFESLKKNSALLGLEGMNDDEITCMLREGDIDGDGALNEMEFCTLMFRLSPALMSDSKQLLEEAIFTGH*